Claims
- 1. A method of detecting the presence of a grapevine leafroll virus in a sample, said method comprising contacting said sample with an antibody or binding portion thereof that specifically binds a polypeptide selected from the group consisting of a helicase, an RNA-dependent RNA polymerase, an hsp70 polypeptide, and an hsp90 polypeptide, and assaying for binding of said antibody or antibody portion thereof to said polypeptide, such binding indicating that said virus is present in said sample.
- 2. The method of claim 1, wherein the antibody or binding portion thereof specifically binds a helicase of the grapevine leafroll virus.
- 3. The method of claim 1, wherein the antibody or a binding portion thereof specifically binds an RNA-dependent RNA polymerase of the grapevine leafroll virus.
- 4. The method of claim 1, wherein the antibody or a binding portion thereof specifically binds an hsp70 polypeptide of the grapevine leafroll virus.
- 5. The method of claim 1, wherein the antibody or a binding portion thereof specifically binds an hsp90 polypeptide of the grapevine leafroll virus.
- 6. The method of claim 1, wherein the antibody is a monoclonal antibody.
- 7. The method of claim 1, wherein the antibody or is a polyclonal antibody.
- 8. A method of detecting the presence of a grapevine leafroll virus in a sample, said method comprising analyzing a nucleic acid of said sample, said analyzing comprising contacting said nucleic acid with a nucleic acid probe that specifically hybridizes to nucleic acid encoding a coat protein, a helicase, or an hsp70 gene of the grapevine leafroll virus and assaying for specific hybridization of said probe to said nucleic acid of said sample, such hybridizing indicating that said virus is present in said sample.
- 9. The method of claim 8, wherein said probe is at least a portion of a coat protein gene of the grapevine leafroll virus.
- 10. The method of claim 8, wherein said probe is at least a portion of a helicase gene of the grapevine leafroll virus.
- 11. The method of claim 8, wherein said probe is at least a portion of an hsp70 gene of the grapevine leafroll virus.
- 12. A method of detecting the presence of a grapevine leafroll virus in a sample, said method comprising analyzing a nucleic acid of said sample, said analyzing comprising contacting said nucleic acid with at least one nucleic acid primer that specifically hybridizes to at least a portion of a coat protein, a helicase, or an hsp70 gene of the grapevine leafroll virus and performing a gene amplification detection procedure on said nucleic acid of said sample, said amplification indicating that said virus is present in said sample.
- 13. The method of claim 12, wherein said primer is at least a portion of a coat protein gene of the grapevine leafroll virus.
- 14. The method of claim 12, wherein said primer is at least a portion of a helicase gene of the grapevine leafroll virus.
- 15. The method of claim 12, wherein said primer is at least a portion of an hsp7o gene of the grapevine leafroll virus.
- 16. The method of claim 12, wherein said gene amplification detection procedure comprises a polymerase chain reaction procedure.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Patent Application Serial No. 60/009,008, filed Dec. 21, 1995.
Government Interests
[0002] This work was supported by U.S.-Israel Binational Agricultural Research and Development Fund Grant No. US-1737-89 and by the U.S. Department of Agriculture Cooperative Agreement No. 58-2349-9-01. The Federal Government may have certain rights in the invention.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60009008 |
Dec 1995 |
US |
Divisions (1)
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Number |
Date |
Country |
Parent |
08770544 |
Dec 1996 |
US |
Child |
09224898 |
Dec 1998 |
US |
Continuations (3)
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Number |
Date |
Country |
Parent |
09650324 |
Aug 2000 |
US |
Child |
10039112 |
Dec 2001 |
US |
Parent |
09579259 |
May 2000 |
US |
Child |
09650324 |
Aug 2000 |
US |
Parent |
09224898 |
Dec 1998 |
US |
Child |
09579259 |
May 2000 |
US |