Claims
- 1. A growth medium comprising a complex at a concentration effective to enhance B cell growth, the complex consisting essentially of prealbumin, retinol-binding protein, and a retinoid which binds to retinol-binding protein, wherein the concentration of retinoid in the complex is from 2.times.10.sup.-5 M to 10.sup.-5 M and the concentration of prealbumin and retinol-binding protein is sufficient to maintain the retinoid concentration from 2.times.10.sup.-5 M to 10.sup.-10 M.
- 2. A method of enhancing the growth of B cells in culture which comprises culturing the B cells in the growth medium of claim 1.
- 3. The method of claim 2, wherein the B cells are human B cells.
- 4. The growth medium of claim 1, wherein the molar ratio of prealbumin, retinol-binding protein, and retinoid is about 1:1:1.
- 5. The growth medium of claim 1, wherein the retinoid is all-trans-retinol.
- 6. The growth medium of claim 1, wherein the retinoid is all-trans-retinal.
- 7. The growth medium of claim 1, wherein the retinoid is 13-cis-retinol.
- 8. The growth medium of claim 1, wherein the retinoid is 13-cis-retinal.
- 9. The growth medium of claim 1, wherein the prealbumin is mammal prealbumin.
- 10. The growth medium of claim 9, wherein the mammal prealbumin is human prealbumin.
- 11. The growth medium of claim 9, wherein the mammal prealbumin is bovine prealbumin.
Parent Case Info
This application is a continuation of 08/283,993 filed Aug. 1, 1994, now abandoned, which is a continuation of 08/007,807 filed Jan. 22, 1993, now abandoned, which is a continuation of 07/387,013 filed Jul. 28, 1989, now abandoned, which is a continuation-in-pan of 07/206,569 filed Jun. 14, 1988, now abandoned.
Government Interests
This invention was made with government support under Grant Number IM-480 from the American Cancer Society. Accordingly, the U.S. Government has certain rights in the invention.
Non-Patent Literature Citations (7)
Entry |
Ambrus et al., "Identification of a cDNA for a Human High-Molecular-Weight B-cell Growth Factor", Proc. Natl. Acad. Sci. USA 90:6330-6334, Jul. 1993. |
Buck et al., "Differences in the Action and Metabolism Between Retinol and Retinoic Acid in B Lymphocytes," J. Cell Biology 115(3):851-859. |
Goodman, D. S., "Plasma Retinol-Binding Protein," in The Reintoids, vol. 2, Academic Press, pp. 41-88. |
Buck et al., "Purification and Biochemical Characterization of a Human Autocrine Growth Factor Produced by Epstein-Barr Virus Transformed B Cells", Journal of Immunology, v. 138, 2923-2928, May 1, 1987. |
Ambrus et al. (A), "Human B Rymphoma cell line producing B cell growth factor", Journal of Clinical Investigation, v. 75, 732-739, 1985. |
Ambrus et al. (B), "Purification to homogeneieth of a high molecular weight human B cell growth factors", J. Exp. Medicine, v. 162, 1319-1335, 1985. |
Campbell, Laboratory Techniques: Monoclonal Antibody Technology, pp. 104.105, 1984. |
Continuations (3)
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Number |
Date |
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Parent |
283993 |
Aug 1994 |
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Parent |
7807 |
Jan 1993 |
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Parent |
387013 |
Jul 1989 |
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Continuation in Parts (1)
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Date |
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Parent |
206569 |
Jun 1988 |
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