Patent Application
20100203055
The present invention relates to a hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia comprising a substance which inhibits the growth of hair follicles (synonymous with hair roots), and a method of screening for such substances.
It is known that a variety of polypeptide growth factors, including various members of the fibroblast growth factor (hereinafter, referred to as “FGF”) family, are expressed in skin tissue. In both mouse and human, FGFs are encoded by 22 distinct genes (Non-Patent Document 1). Among them, FGF1, FGF2, FGF5, FGF7, FGF10, FGF13 and FGF22 are reported to be expressed in dermal cells and hair follicular cells to regulate hair growth and skin regeneration (see Non-Patent Documents 2-17 and Patent Document 1).
Non-Patent Document 2-17 and other documents suggest that FGF plays an important role in the growth and differentiation of cutaneous cells. However, it is still unknown as to how the FGF group is involved in the effect of promoting the growth of hair follicles and the resultant hair growth promoting effect and hair regrowth promoting effect.
Under the above-described circumstances, the present inventors found that single administration of FGF18 to mouse skin with hair folliclles in telogen phase (resting phase) induces hair regrowth, and reported that FGF18 is a substance which induces onset of anagen phase (growth phase) in follicles to result in promotion of hair growth (Non-Patent Document 19 and Patent Document 2).
Non-Patent Document 1: Ornitz D M, Itoh N: Fibroblast growth factors. Genome Biol2: REVIE WS3005, 2001
Non-Patent Document 2: du Cros D L: Fibroblast growth factor and epidermal growth factor in hair development. J Invest Dermatol 101: 106S-113S. 1993
Non-Patent Document 3: du Cros D L, Isaacs K, Moore G P: Distribution of acidic and basic fibroblast growth factors in ovine skin during follicle morphogenesis. J Cell Sci 105: 667-674, 1993
Non-Patent Document 4: Herbert J M, Rosenquist T, Gotz J, Martin G R: FGF5 as a regulator of the hair growth cycle: Evidence from targeted and spontaneous mutations. Cell 78: 1017-1025, 1994
Non-Patent Document 5: Danilenko D M, Ring B D, Yanagihara D, Benson W, Wiemann B, StarnesCO, Pierce G F: Keratinocyte growth factor is an important endogenous mediator of hair follicle growth, development, and differentiation. American J Pathol 147: 145-154, 1995
Non-Patent Document 6: Marchese C, Chedid M, Dirsch O R, et al: Modulation of keratinocyte growth factor and its receptor in reepithelializing human skin. J Exp Med 182: 1369-1376, 1995
Non-Patent Document 7: Guo L, Degenstein L, Fuchs E: keratinocyte growth factor is required for hair development but not for wound healing. Genes Dev 10: 165-175, 1996
Non-Patent Document 8: Rosenquist T A, Martin G R: Fibroblast growth factor signalling in the hair growth cycle: Expression of the fibroblast growth factor receptor and ligand genes in the murine hair follicle. Developmental Dynamics 205:379-386, 1996
Non-Patent Document 9: Petho-Schramm A, Muller H J, Paus R: FGF5 and the murine hair cycle. Arch Dermatol Res 288: 264-266, 1996
Non-Patent Document 10: Mitsui S, Ohuchi A, Hotta M, Tsuboi R, Ogawa H: Genes for a range of growth factors and cyclin-dependent kinase inhibitors are expressed by isolated human hair follicles. Br J Dermatol 137:693-698, 1997
Non-Patent Document 11: Ortega S, Ittmann M, Tsang S H, Ehrlich M, Basilico C: Neuronaldefects and delayed wound healing in mice lacking fibroblast growth factor-2. Proc Natl Acad Sci USA 95: 5672-5677, 1998
Non-Patent Document 12: Suzuki S, Kato T, Takimoto H, et al: Localization of rat FGF-5 protein in skin macrophage-like cells and FGF-5S protein in hair follicle: Possible involvement of two Fgf-5 gene products in hair growth cycle regulation. J Invest Dermatol 111: 963-972, 1998
Non-Patent Document 13: Suzuki S, Ota Y, Ozawa K, Imamura T: Dual-mode regulation of hair growth cycle by two Fgf-5 gene products. J Invest Dermatol 114: 456-463, 2000
Non-Patent Document 14: Nakatake Y, Hoshikawa M, Asaki T, Kassai Y, Itoh N: Identification of a novel fibroblast growth factor, FGF-22, preferentially expressed in the inner root sheath of the hair follicle. Biochem Biophys Acta 1517: 460-463, 2001
Non-Patent Document 15: Stenn K S, Paus R: Controls of hair follicle cycling. Physiol Rev 81: 449-494, 2001
Non-Patent Document 16: Beyer T A, Werner S, Dickson C, Grose R: Fibroblast growth factor 22 and its potential role during skin development and repair. Exp Cell Res 287: 228-236 2003
Non-Patent Document 17: kawano M, Suzuki S, Suzuki M, Oki J, Imamura T: Bulge- and basal layer-specific expression of fibroblast growth factor 13(FHF-2) in mouse skin. J Invest Dermatol 122: 1084-1090, 2004
Non-Patent Document 18: Suzuki S, Ota Y, Ozawa K, Imamura T: Dual-mode regulation of hair growth cycle by two Fgf-5 gene products. J Invest Dermatol 114: 456-463, 2000
Non-Patent Document 19: Kawano M, Komi-Kuramochi A, Asada M, Suzuki M, Oki J, Jiang J, Imamura T: Comprehensive analysis of FGF and FGFR expression in skin: FGF18 is highly expressed in hair follicles and capable of inducing anagen from telogen stage hair follicles. J Invest Dermatol. 2005, 124(5): 877-885
Patent Document 1: Japanese Unexamined Patent Publication No. Hei 4-224522
Many people are suffering from thin hair or hair loss. However, there is no hair regrowth treatment agent effective for everyone. It is an object of the present invention to elucidate the mode of action of endogenous factors that regulate hair growth, to determine an endogenous factor that inhibits hair growth, to screen for substances that inhibit the activity or expression of the endogenous factor, and to provide a novel hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia.
It is another object of the present invention to provide a screening method for the above-described purpose.
As described above, it was already confirmed that single administration of FGF18 to skin with hair follicles in telogen phase promotes hair growth after several weeks of reaction period. Therefore, FGF18 was believed to be a substance that has a hair growth promoting effect.
However, the present inventors have obtained an unexpected, surprising finding when FGF18 was administered to mouse dorsal skin subcutaneously once a day for 8 days after compulsive induction of anagen phase in follicles of the dorsal skin in telogen phase by depilating, so that FGF18 was allowed to persist in hair follicles continuously for observing the state of growth of hair.
Briefly, hair growth progressed well and follicle enlargement occurred at day 9 in the control group which received phosphate-buffered physiological saline under similar conditions whereas hair growth was markedly inhibited the FGF18 administered group.
When administration of FGF18 was stopped after its continuous administration under the same conditions and mice were raised thereafter, growth of hair follicles occurred.
Further, the present inventors performed a detailed functional analysis of FGF18. As a result, it was found that the expression level of a growth factor VEGF (which is considered important for hair follicle growth) increased when dermal papilla cells (which are believed to be a control tower for hair follicle growth) were cultured in the presence of FGF18 and then the medium is deprived of FGF18.
Based on these findings, the previous results of animal experiments can be rather explained as having observed “the effect of decreased FGF18 concentration” that resulted from rapid degradation/invalidation of the singly administered FGF18 protein by the mouse body. Therefore, the present inventors assumed that the continuous presence of FGF18 acts inhibitory against hair growth, and this assumption has been examined experimentally. As a result, this assumption was demonstrated to be correct. Thus, the present invention has been achieved.
Considering that FGF18 is an endogenous factor present in hair follicles by nature, both a substance that inhibits the activity of FGF18 in hair follicles and a substance that inhibits the expression of FGF18 can be used as a hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia.
Substances which inhibit the expression of FGF18 may be screened for by monitoring whether a test substance promotes the expression of FGF18 or not in a cultured animal cell or an experimental animal.
By developing the above-described finding further, the present inventors assumed that a substance which works as an antagonist to FGF18 by inhibiting the binding of FGF18 to FGF receptors would inhibit the activity of FGF18 and thus such a substance would have a hair growth promoting effect. Further, the present inventors assumed that an antibody which binds to FGF18 to thereby inhibit the binding of FGF18 to FGF receptors would inhibit the activity of FGF18 and thus such an antibody would have a hair growth promoting effect. Since FGF18 reacts with at least four FGF receptor subclasses, i.e., FGFR1c, FGFR2c, FGFR3c and FGFR4 (Non-Patent Document 20), the present invertors particularly noted the above reactivity to develop a screening system for substances that inhibited the activity of FGF18.
As a result of extensive searches into substances derived from naturally occurring plants and other natural product-derived substances using FGFR3c and/or FGFR4 receptor as the screening system, the present invertors found several natural product-derived substances which would inhibit the activation of FGFR3c and/or FGFR4 receptor by FGF18. Thus, the present invention relating to the substance that inhibits the activity of FGF18 has also been achieved.
The present invention includes the following inventions.
(1) A hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia, comprising as an active ingredient(s) a substance that inhibits the activity of FGF18 and/or a substance that inhibits the expression of FGF18.
(2) The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to (1), wherein the substance that inhibits the activity of FGF18 is a partial peptide of FGF18.
(3) The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to (1), wherein the substance that inhibits the activity of FGF18 is an anti-FGF18 antibody.
(4) The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to (1), wherein the substance that inhibits the activity of FGF18 is a Momordica charantia extract.
(5) The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to (1), wherein the substance that inhibits the activity of FGF18 is an expression vector in which a cDNA encoding a partial peptide of FGF18 having an inhibitory activity against the activity of FGF18 has been integrated.
(6) The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to (1), wherein the substance that inhibits the expression of FGF18 is an expression vector in which an siRNA having an inhibitory activity against the expression of FGF18 has been integrated.
(7) The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to any one of (1) to (6), further comprising an other hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia, respectively.
(8) A method of screening for the substance that inhibits the activity of FGF18 according to any one of (1) to (4) to thereby obtain candidates for the hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia, the method comprising the following steps (a) to (c):
(a) compulsively expressing at least one FGF receptor gene selected from FGFR1c, FGFR2c, FGFR3c and FGFR4 on the surface of a cell by means of genetic engineering and culturing the cell,
(b) bringing, together with FGF18, a test substance into contact with the cell system obtained in step (a) having the FGF receptor on cell surfaces; and
(c) selecting those test substances which exhibited an inhibitory activity against the cell growth promoting activity of FGF18 in step (b).
(9) The method of (8), wherein the FGF receptor is FGFR3c.
(10) The method of (8), wherein the FGF receptor is FGFR4.
(11) The method of any one of (8) to (10), wherein the cell on whose surface the FGF receptor of (8) is compulsively expressed is mouse IL-3-dependent Ba/F3 cell strain.
(12) A method of screening for the substance that inhibits the expression of FGF18 according to (1) to thereby obtain candidates for the hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia, the method comprising the following steps (a) to (d):
(a) preparing a cultured animal cell or an experimental animal capable of expressing FGF18 to an observable extent,
(b) bringing a test substance into contact with the cultured animal cell of (a), or bringing a test substance into contact with or administering the same to the experimental animal of (a);
(c) monitoring the expression of FGF18 in the cultured animal cell or the experimental animal after step (b), and
(d) selecting those test substances which have a function of inhibiting the expression of FGF18.
(13) The method of (12), wherein the expression of FGF18 is monitored in step (c) by extracting mRNA from the experimental animal or the cultured animal cell after step (b) and then analyzing the mRNA level of expressed FGF18; and those test substances which have a function of inhibiting the expression of FGF18 are selected in step (d) by selecting systems that exhibited lower levels of FGF18 mRNA than when the test substance was not allowed to act.
The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia as defined in (1) to (7) above may further comprise other protein growth factors and/or hair growth promoting agents.
Examples of other protein growth factors include epidermal growth factor, platelet-derived growth factor, factors other than FGF18 belonging to the FGF family, transforming growth factor-α, transforming growth factor-β, factors belonging to the transforming growth factor β superfamily, insulin-like growth factor-I and insulin-like growth factor-II. The agent or therapeutic of (1) to (7) above may comprise one or several of the above-listed protein growth factors. It should be noted that other protein growth factors are not limited to those listed above.
Examples of other hair growth promoting agents include, but are not limited to, minoxidil, minoxidil analogues, minoxidil derivatives, antiandrogenic agents and a 5α-reductase inhibitor finasteride (Propecia). The agent or therapeutic of (1) to (7) above may comprise one or several of the above-listed hair growth promoting agents.
According to the present invention, it is possible to provide a hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia that act by suppressing an endogenous factor that inhibits the growth of hair follicles. With the hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to the present invention, hair growth, promotion of hair regrowth and treatment of alopecia in bald or thin-haired head can be achieved more effectively.
Further, according to the screening method of the present invention, it is possible to screen for substances that are effective as hair growth promoting agents, hair regrowth promoting agents or therapeutics for alopecia.
A: Test sample was added in 1 μg/ml. <Control> Column 1: No sample added, aside from FGF18. <R3c> Column 2: (d4). Column 3: (d16). Column 4: (d18). Column 5: (d22). Column 6: (d37). Column 7: (d48). Column 8: (d77). Column 9: (d95). <R4> Column 10: (d22). Column 11: (d37). Column 12: (d48). Column 13: (d67). Column 14: (d77). Column 15: (d95).
B: Test sample was added in 100 ng/ml. <Control> Column 1: No sample added, aside from FGF18. <R3c> Column 2: (d4). Column 3: (d12). Column 4: (d16). Column 5: (d18). Column 6: (d37). Column 7: (d48). Column 8: (d67). Column 9: (d95). <R4> Column 10: (d37). Column 11: (d67).
FGFR4/BaF3 cells were cultured in the presence of FGF18 with the addition of Momordica charantia hot water extract. Inhibitory effect of Momordica charantia hot water extract against the FGFR4/BaF3 cell growth promoting effect of FGF18 is shown for the case where the extract was added at 0.083%, 0.83% and 8.3%.
Ethanol solution of Momordica charantia hot water extract (containing 49.5% ethanol and 1% glycerol) was applied to the dorsal skin of C3H/He mice in telogen phase of the hair cycle daily for 11 days (except for day 5 and day 6). The state of hair regrowth in the mouse dorsal skin on days 10, 14, 17, 21 and 24 is shown in hair regrowth scores.
Hereinbelow, the present invention will be described in detail.
By applying the present invention, it is possible to provide a novel hair growth promoting agent, hair regrowth promoting agent and therapeutic for alopecia. These agents and therapeutics share the common feature that they inhibit the activity or expression of FGF18 and that they utilize the action of sustained high concentrations of FGF18 for inhibiting the growth of hair follicles (sometimes called “hair roots”).
The hair follicle is an organ that produces hair. The hair follicle growth cycle consists of a growth phase (anagen), a recessing phase (catagen) and a resting phase (telogen) which follows the recessing phase. After the resting phase, the growth phase begins. Generally, in mouse experimental systems, anagen phase is a period from day 1 to day 19 after depilation; and catagen phase is a period from day 20 to day 21 after depilation. It is also known that the hair follicle cycle enters telogen phase at day 21-22 after depilation. During the anagen phase, the growth (elongation) of new hair is activated. Simultaneously, the growth of hair follicles is activated inside the skin, and the bottom part of hair follicles reaches the vicinity of the skin bottom. On the other hand, during the telogen phase, hair follicles stay shallow in the skin in small sizes. Further, the thickness of the skin is completely different between anagen phase and telogen phase. In mice with a colored body hair, melanin is synthesized at the beginning of the anagen phase and a blue skin is visible. Therefore, it is also possible to evaluate the progress of hair follicle growth cycle by observing the blue color from the outside of the skin. Further, when the skin is dissected during the anagen phase and observed from the reverse side, the reverse side of the skin can be seen black because hair follicles with abundant melanin are standing in lines at a high density. To the contrary, during the telogen phase, the reverse side of the skin can be seen remaining white. For example, in mouse experimental systems, the entire dorsal hair of 7 to 8 week-old mice is in telogen phase; by depilating the grown hair, the hair cycle is synchronized and anagen phase begins.
1. Substances that Inhibit the Activity of FGF18
The substance that inhibits the activity of FGF18 and which is contained in the novel hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to the present invention may, for example, be a partial fragment of FGF18.
FGF18 is synthesized in the cytoplasm of FGF18 producing cells as a polypeptide of 207 amino acids in human and mouse. When this polypeptide is secreted to the outside of cells, its N-terminal signal peptide is cleaved to generate a secreted polypeptide of 181 amino acids which exerts a physiological action. This polypeptide reacts with at least four out of the seven FGF receptor subclasses (FGFR1c, FGFR1b, FGFR2c, FGFR2b, FGFR3c, FGFR3b and FGFR4) and they are FGFR1c, FGFR2c, FGFR3c and FGFR4.
The term “FGF18” as used in the present invention refers not only to human-derived FGF18. Other FGF18 polypeptides derived from, for example, other mammals may also be used. Specific examples of other mammals include, but are not limited to, mouse, rat, chicken, turkey, cattle, pig, sheep and rabbit. For example, it is possible to isolate a gene encoding FGF18 from a non-human mammal by designing a probe based on the nucleotide sequence of a human-derived FGF18 as shown in SEQ ID NO: 1 and using the probe according to conventional methods.
The nucleotide sequence and the amino acid sequence of the signal sequence-deleted human-derived FGF18 are shown in SEQ ID NOS: 1 and 2, respectively. The nucleotide sequence and the amino acid sequence of the signal sequence-deleted mouse-derived FGF18 are shown in SEQ ID NOS: 3 and 14, respectively. As comparison of the amino acid sequences as shown in SEQ ID NOS: 2 and 14 reveals, FGF18 polypeptides have a very high homology in mammals. Thus, it is understood that the function of FGF18 is almost the same among mammals.
Generally, the term “antagonist” refers to a substance that binds to a receptor but, unlike a biological substance (ligand) that stimulates the receptor, does not provoke a biological response or provokes only a relatively weak biological response; alternatively, it is a substance that inhibits the binding between the receptor and its endogenous binding partner to thereby block or dampen biological responses. Since FGF18 reacts with at least four FGF receptor subclasses, FGFR1c, FGFR2c, FGFR3c and FGFR4, among the seven subclasses as described above, the term “FGF18 antagonist effect” as used herein means the effect of a substance which blocks or dampens the response by FGF18 in cells that express any of the following receptors: FGFR1c, FGFR2c, FGFR3c and FGFR4. An FGF18 antagonist inhibits the binding of FGF18 to its receptor, which suppresses the hair growth inhibiting effect resulting from FGF18. Consequently, it can be said that the FGF18 antagonist has a hair growth promoting effect.
With respect to partial peptides of FGF18 having an EGF18 antagonist effect, take human FGF18 as an example; the region spanning from positions 32 to 151 of the amino acid sequence shown in SEQ ID NO: 2 is a region called the core domain which is highly common to the FGF family, and a three-dimensional structure that is solely constructed of this region is believed to have a basic ability to bind to the receptor but not a complete activity; hence, partial peptides comprising the amino acid sequence spanning the region corresponding to the core domain are believed to have an FGF18 antagonist effect. If FGF18 partial peptides comprising the amino acid sequence corresponding to the core domain have a sufficient ability to bind to receptors such as FGFR4 but do not have a sufficient length to provoke FGF18 response, such partial peptides have an EGF18 antagonist effect.
Actually, according to the results confirmed in experiments and shown in
Antibodies that bind to FGF18 or FGF18 reactive receptors (i.e., anti-FGF18 antibody or anti-FGFR3c antibody, anti-FGFR4 antibody, etc.) also inhibit the binding of FGF18 to its receptors. Thus, these antibodies inhibit the hair follicle growth inhibiting effect of FGF18.
Thus, in the present invention, substances that inhibit the receptor-mediated effect of FGF18 (such as substances that inhibit the binding of FGF18 to its receptors, including the above-described FGF18 antagonists) are referred to as the “substance that inhibits the activity of FGF18”.
Such substances having an inhibitory effect against FGF18 activity may be those selected from a group of substances that have nothing to do with FGF18. By applying the screening method described in subsection 2 below, substances having an inhibitory effect against FGF18 activity can be obtained easily. In Examples of the present invention, a Momordica charantia extract having an inhibitory effect against FGF18 activity was selected by the screening method, and it was confirmed that the Momordica charantia extract inhibits the hair follicle growth inhibitory effect of FGF18 and has a hair growth effect. Thus, the substance that inhibits the activity of FGF18 according to the present invention inhibits the binding of FGF18 to its receptors to thereby inhibit the hair follicle growth inhibitory effect of FGF18. Consequently, that substance has a hair growth promoting effect.
When the substance that inhibits the activity of FGF18 is a peptide, protein or glycoprotein, the substance may be of course administered in the form of a preparation containing such a peptide or the like. Alternatively, that substance may be administered as an expression vector in which a DNA encoding the peptide or the like has been integrated. For example, a preparation whose active ingredient is an expression vector in which an FGF18 partial peptide-encoding DNA has been integrated may be used as a hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia.
These substances that inhibit the activity of FGF18 may be used alone or in combination as a hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia.
2. Screening Method for Substances that Inhibit the Activity of FGF18
A method of screening for substances that inhibit the activity of FGF18 to give candidates for the hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia comprises specifically the following steps (a) to (c).
(a) compulsively expressing at least one FGF receptor gene selected from FGFR1c, FGFR2c, FGFR3c and FGFR4 on the surface of a cell by means of genetic engineering and culturing the cell,
(b) bringing, together with FGF18, a test substance into contact with the cell system obtained in step (a) having the FGF receptor on cell surfaces; and
(c) selecting those test substances which exhibited an inhibitory activity against the cell growth promoting activity of FGF18 in step (b).
As the FGF receptor gene, at least one of the FGF receptor genes FGFR1c, FGFR2c, FGFR3c and FGFR4 is used; it has been confirmed that FGF18 binds to these receptors and that FGF18 exerts cell growth effect upon cells having these receptors on their surfaces. Preferably, FGFR3c or FGFR4 is used. To bring a test substance into contact with a cell in step (b), it is typically directly added to the cell culture broth, but in a particular case where the test substance is a protein, a gene encoding the test substance can be transferred into an FGF receptor-expressing cell.
The cell to be used for compulsive expression of an FGF receptor on its surface may be any cell as long as it can be cultured. Preferably, mouse IL-3-dependent Ba/F3 cell strain is used.
In the screening, cells are cultured for 48 hr or more (e.g., about 72 hr) and then those substances which decreased the proliferation capacity of FGF receptor expressing cells by 5% or more, preferably by 10% or more, compared to the addition of FGF18 alone may be selected.
The parent cell with no FGF receptor gene transferred thereinto may be used in control test. It is preferable to provide a step in which the same procedure as in step (b) is performed, except that FGF18 added together with a test substance is replaced by IL-3, to confirm that the test substance does not inhibit the cell growth promoting activity of IL-3 in the parent cell.
3. Substances that Inhibit the Expression of FGF18 and Screening Method for such Substances
Since FGF18 is an endogenous factor present in hair follicles, a substance that inhibits the transcription and translation of FGF18 in hair follicle cells (i.e., substance that decreases the expression of FGF18) is also capable of decreasing the concentration of FGF18 in hair follicle cells to thereby induce the hair growth promoting effect of FGF18. Thus, such a substance has a hair growth promoting effect and a hair regrowth promoting effect.
Such substances that inhibit the expression of FGF18 may be designed as an siRNA against FGF18 or its expression vector by known methods. The activity of such substances may be confirmed by monitoring whether or not a test substance inhibits the expression of FGF18 in cultured animal cells or experimental animals.
Alternatively, such substances that inhibit the expression of FGF18 may be screened for by monitoring whether or not a test substance inhibits the expression of FGF18 in cultured animal cells or experimental animals.
Specifically, first, cultured animal cells or experimental animals capable of expressing FGF18 to an observable extent are prepared. Then, a test substance is brought into contact with or administered to the experimental animal, or a test substance is brought into contact with the cultured animal cell. Experimental animals refer to non-human animals such as mouse, rat, chicken, turkey, cattle, pig, sheep and rabbit. Examples of test substances include, but are not limited to, plant extracts, peptides, proteins, nonpeptidic compounds, low molecular weight compounds, synthetic compounds, fermentation products, cell extracts and animal tissue extracts. These substances may be either novel substances or known substances. To bring it into contact with a cell or experimental animal, the test substance is typically directly added to the cell culture broth or administered to the animal but in a particular case where the test substance is a protein, a gene encoding the test substance can be transferred into an FGF receptor-expressing cell.
Subsequently, the expression of FGF18 in the cultured animal cell or experimental animal is monitored. The expression of FGF18 in the cultured animal cell or experimental animal may be monitored, for example, by analysis with conventional methods such as ELISA using FGF18 antibody or by analyzing the FGF18 mRNA level in the cell or experimental animal by quantitative reverse transcription PCR or Northern blotting.
If, as a result of analysis by any of the above-listed methods, the expression level of FGF18 in the cultured animal cell or experimental animal cultured in the presence of a test substance is found to be lower than that level in the animal cell cultured in the absence of the test substance, the test substance may be judged to have a potential function of hair growth promotion or hair regrowth. Specifically, if the mRNA level decreased to 0.8-fold or less, preferably to 0.7-fold or less, more preferably to 0.5-fold or less, compared to the level for the case whether the test substance was not allowed to act, the latter can positively be regarded as a substance that inhibits the expression of FGF18. The expression levels of FGF18 mRNA in cultured keratinocytes, cultured dermal cells or cultured dermal papilla cells vary considerably depending on culture conditions or the type of the cells. So they may be measured individually by the above-mentioned methods or the like and those test substances that 0.8-fold or less decreases in measured values may be used as a criterion for screening.
The FGF18 expression inhibiting substance screened through the above-described steps may be used either alone or in combination as a hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia.
The substances described in above subsections “1. Substances that Inhibit the Activity of FGF18” and/or “3. Substances that Inhibit the Expression of FGF18” are formulated into preparations adapted for skin application (e.g., solutions, creams, ointments, gels, lotions, shampoos or aerosols) and are supplied as hair growth promoting agents, hair regrowth promoting agents or therapeutics for alopecia.
In particular, the hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia is administered in the form of a pharmaceutical composition comprising a substance that inhibits the activity of FGF18 and/or a substance that inhibits the expression of FGF18 together with a pharmacologically acceptable carrier adapted for local application. The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia comprising a substance that inhibits the activity of FGF18 and/or a substance that inhibits the expression of FGF18 contains the active compound(s) in a pharmacologically acceptable carrier usually at about 0.01 to about 100 μg/day/cm2, preferably about 0.1 to about 10 μg/day/cm2. This means that the concentration of FGF18 is usually about 0.01 to about 100 μg/day/cm2, preferably about 0.1 to about 10 μg/day/cm2 of the active compound in a pharmacologically acceptable carrier.
Further, the pharmacologically acceptable carrier adapted for local application is not particularly limited. Specific examples include, but are not limited to, ointments such as hydrophilic vaseline or polyethylene glycol ointment; pastes such as gum (e.g., xanthan gum); solutions such as alcoholic, aqueous or buffer solution; gels such as aluminum hydroxide or sodium alginate gel; albumins such as human or animal albumin; collagens such as human or animal collagen; celluloses such as alkyl cellulose, hydroxyalkyl cellulose or alkylhydroxyalkyl cellulose; methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, hydroxypropylmethyl cellulose and hydroxypropyl cellulose; polymers such as Pluronic™ polyol as exemplified by Pluronic F-127; tetronics such as Tetronic 1508; and alginates such as sodium alginate.
As the substance that inhibits the activity of FGF18 according to the present invention, an expression vector may be used in which a DNA encoding an FGF18 activity inhibitory protein or peptide (such as a partial peptide of FGF18) has been integrated. In that case, the expression vector may be supplied in such a form that it is used in a conventional gene therapy. The expression vector is provided with sequences such as promoter to drive the expression of a partial peptide of FGF18 or the like in animal cells, but is not particularly limited. Examples of expression vectors which may be used in the present invention include, but are not limited to, plasmid vectors and virus vectors.
The hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to the present invention may comprise a substance that inhibits the expression of FGF18 as an active ingredient. Briefly, the hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia according to the present invention may be supplied as in gene therapy using a vector for expression of siRNA against FGF18. The expression vector is provided with sequences such as promoter to drive the expression of siRNA in animal cells, but is not particularly limited. Examples of expression vectors which may be used in the present invention include, but are not limited to, plasmid vectors and virus vectors. It should be noted here that a substance which inhibits the expression of FGF18 is not limited to the vector for expression of siRNA against FGF18.
As a method of introducing the hair growth promoting agent, hair regrowth promoting agent or therapeutic for alopecia for use in gene therapy into cells, both a gene transfer method using a virus vector and a non-viral, gene transfer method [Nikkei Science, 1994 April issue, pp. 20-45; Experimental Medicine Extra Issue 12(15)(1994); Experimental Medicine Separate Volume “Basic Techniques for Gene Therapy”, Yodo-sha (1996)] may be used.
As examples of gene transfer method using a virus vector, methods may be mentioned in which a DNA encoding TR4 or mutant TR4 is integrated in DNA or RNA viruses such as retrovirus, adenovirus, adeno-associated virus, herpes virus, vaccinia virus, poxvirus, poliovirus and sinbis virus. Among these, methods using retrovirus, adenovirus, adeno-associated virus or vaccinia virus are especially preferred.
Examples of non-viral gene-transfer methods include a method in which an expression plasmid is directly administered into muscle (DNA vaccine method), the liposome method, lipofection, microinjection, the calcium phosphate method, electroporation and so on. The DNA vaccine method and the liposome method are especially preferred.
It is possible to construct an in vitro hair regeneration system in a regenerated skin tissue using a substance that inhibits the activity of FGF18 and/or a substance that inhibits the expression of FGF18. The term “regenerated skin tissue” as used herein means a tissue consisting of various types of skin cells obtainable by culturing isolated skin stem cells. Various types of skin cells are not particularly limited. They include, for example, epithelial cell in the epidermis, cells in the epithelium basal layer, various cells constituting the hair follicle, dermal cells, adipocytes, and so on. Cells used for regenerating a skin tissue may be any of the following cells: heterologous cells, allogeneic cells and autologous cells.
First, there is no established technology that can be used as the method of regulating the differentiation from skin stem cells into various types of skin cells. Therefore, the method is not particularly limited in the present invention. For example, growth factor receptors that exhibit different expressions at the stage of spontaneous differentiation may be used in such a way that ligand growth factors corresponding to the respective receptors are added into a medium to thereby achieve selective amplification of cells having different directions of differentiation. After selective amplification of the cells having different directions of differentiation, a skin tissue may be prepared.
Since there is no established technology for preparing artificial skin tissues, the method of preparation is not particularly limited in the present invention. Various methods may be used and examples include: a method in which epithelial cells alone are cultured and layered; a method in which the dermal layer is formed with dermis-constituting cells (such as fibroblast cells) and then epithelial cells are over-layered to form an integral sheet; a method in which the surface of the over-layered epithelial cells is exposed to the air to promote the formation of epidermis; or a method in which a layered film formed of a biodegradable component is used instead of the dermal layer. In the present invention, a skin tissue preparation method used in “regenerative therapy” may also be applied; e.g., skin stem cells are isolated from a skin tissue collected from a human, and a new skin tissue is prepared from the resultant skin stem cells. At this time, the new skin tissue may be prepared on the assumption that it is to be returned to the human donor for the purpose of treatment, or on the assumption that it is to be transplanted to a human different from the human donor for the purpose of treatment.
In such a skin tissue preparation method, if a substance that inhibits the activity of FGF18 and/or a substance that inhibits the expression of FGF18 is added to the medium at an appropriate time, it is possible to promote hair follicle growth in the regenerated skin tissue and to thereby promote hair growth or regrowth in the skin tissue. Further, in a skin tissue preparation method, if a substance that inhibits the activity of FGF18 and/or a substance that inhibits the expression of FGF18 is added to the medium at an appropriate time, it is possible to promote the growth of skin cells in the regenerated skin tissue and to thereby increase the volume of the entire skin tissue.
6. Momordica charantia Extract
One of the substances that inhibit the activity of FGF18 obtained by the screening method of the present invention described in subsection 2. above was an Momordica charantia extract. As shown in Examples described later, the regrowth promoting effect of the extract has been confirmed.
Momordica charantia, the raw material of this extract is a plant belonging to the genus Momordica, the family Cucurbitaceae and is also called Goya, bitter melon or Tsuru-Reishi.
In obtaining an extract from Momordica charantia, the entire part of this plant may be used; individual parts may be used either independently or in an appropriate combination. Further, the plant may be used whether it is in a dry or non-dry state.
For obtaining an extract to be used in the present invention from Momordica charantia, the raw material may be shredded into pieces or crushed and then extracted with an appropriate solvent by conventional extraction methods. The solvent used is not particularly limited. For example, water or anhydrous or hydrous organic solvents may be enumerated. Examples of anhydrous or hydrous organic solvents include one or more substances selected from the group consisting of monohydric alcohols, polyhydric alcohols or derivatives thereof, ketones, esters, ethers, petroleum ether, aliphatic hydrocarbons, halogen compounds and aromatic hydrocarbons. Specific examples of the solvent include, but are not limited to, water, methanol, ethanol, butanol, acetone and ethyl acetate ester. They may be used either alone or in combination. Among these, use of water or a monohydric alcohol such as methanol or ethanol is especially preferable. For extracting purposes, the amounts of the above-listed solvents are not particularly limited. The amount of the solvent may be 0.1-1000 times, preferably 1-100 times, more preferably 2-50 times by weight, the amount of the raw material Momordica charantia.
The extraction method using the above-listed solvents may be performed according to conventional procedures. For example, as regards the extraction temperature, extraction may be performed at around room temperature or at a temperature around the boiling point of the solvent used. As regards the extraction operation, a dried and crushed or a simply crushed Momordica charantia may be soaked in a solvent at room temperature for 1-30 days or may be extracted under reflux at a temperature around the boiling point of the solvent.
As will be described later in one Example, the Momordica charantia extract has an FGF18 inhibitory activity (i.e., inhibits the growth promotion by FGF18) in FGFR4-expressing cells. In the Example, when a cell which had been engineered to express FGFR4 (one of FGF receptors) compulsively on its surface was used, the addition of Momordica charantia extract in the presence of FGF18 was shown to inhibit the FGF18-caused cell growth in a concentration-dependent manner. This effect of Momordica charantia extract was not observed on cell growth in the absence of FGF18 in an simultaneously performed experiment. Therefore, it was indicated that the effect of Momordica charantia extract is not a cytotoxic effect but an FGF18 activity inhibitory effect.
Accordingly, the Momordica charantia extract of the present invention is an FGF18 activity inhibitory substance that inhibits the cell growth by FGF18.
Hereinbelow, the present invention will be described with reference to the following Examples. However, the technical scope of the present invention is not limited to these Examples.
In this Example, in order to evaluate the function of FGF18 on hair growth, the effect of FGF18 on gene expression was examined in dermal papilla cells which are believed to be a control tower for hair follicle growth.
Cultured human dermal papilla cells (HFDP; from adult human scalp; Toyobo) were subcultured in HFDP growth medium (20% fetal bovine serum-containing basal medium for dermal papilla cell; Toyobo) and used in experiments within two passages. The cells were treated with trypsin and seeded in collagen-coated dishes (Sumilon; 6 cm in diameter) at 4×105 cells/dish. The cells were maintained at 37° C. in HFDP medium. On the next day, the medium was exchanged for HEK medium (EpiLife™, 0.06 mM CaCl2, 10 μg/ml insulin, 0.1 ng/ml hEGF, 0.5 μg/ml hydrocortisone, 0.4% BPE), and then FGF18 was added to the medium. After the cells were cultured for 24 hr, the medium was exchanged for FGF18 free medium in the test sample group (“-FGF18”). The control group (“Control”) was cultured continuously in the FGF18-containing medium. Subsequently, the cells were harvested and the mRNA was extracted and purified. The expression levels of mRNA from VEGF (known as a hair follicle growth promoting factor) and noggin (known as a hair growth inhibiting factor) contained in the resultant mRNA were analyzed.
The results are shown in
In this Example, in order to examine the effect of FGF18 in vivo on hair follicle growth, the effect of FGF18 administration was tested in hair follicle anagen phase-induced C3H/HeN mice.
In order to examine the effect of FGF18 in vivo on hair follicle growth, FGF18 protein dissolved in phosphate buffered physiological saline (PBS) was administered to hair follicle anagen phase-induced mice.
Briefly, dorsal hair of 50 day-old C3H/HeN male mice in telogen phase was depilated gently with fingers to thereby induce the start of hair follicle anagen phase. Then, FGF18 solution was injected into the dorsal skin subcutaneously from the vicinity of the tail (1 μg of FGF18 per mouse). The mice were maintained on a diet and water ad libitum. Starting from this day, FGF18 solution was injected subcutaneously into the dorsal skin every day at about the same time for consecutive 8 days. The control group received injection of PBS instead of FGF18. Nine days after the initial injection, the mice were euthanized under anesthesia. Full thickness skin samples were excised from the dorsal part and embedded in paraffin. The thus embedded skin samples were sliced into 4 μm thick sections with a microtome, stained with hematoxylin and observed under microscope.
The results are shown in
As seen from photomicrograph A, natural growth of hair follicles was observed at day 9 in the PBS-administered mouse. Hair follicles had grown long and reached the lower layer of the skin.
On the other hand, in the FGF18 solution-administered mouse in photomicrograph B, hair follicles are short, suggesting that hair growth is strongly inhibited.
From these results, it was demonstrated that continuous administration of FGF18 inhibits hair growth. Conversely, this suggests that hair follicle growth, if it is being inhibited by continuous presence of endogenous FGF18, can be promoted by inhibiting the activity of FGF18.
As FGF18 partial peptides, partial polypeptides were prepared based on SEQ ID NO: 14 which corresponds to the amino acid sequence of mouse FGF18. Briefly, from up to position 4 to up to position 95 amino acids as counted from the N-terminus (excluding the methionine introduced for initiation of translation) were deleted to prepare partial peptides d4-d95 [indicating the number of amino acids deleted from N-terminal (excluding methionine)].
The amino acid sequences (nucleotide sequences) of the individual partial peptides correspond to the following SEQ ID NOS.
d4: SEQ ID NO: 15 (SEQ ID NO: 4)
d12: SEQ ID NO: 16 (SEQ ID NO: 5)
d16: SEQ ID NO: 17 (SEQ ID NO: 6)
d18: SEQ ID NO: 18 (SEQ ID NO: 7)
d22: SEQ ID NO: 19 (SEQ ID NO: 8)
d37: SEQ ID NO: 20 (SEQ ID NO: 9)
d48: SEQ ID NO: 21 (SEQ ID NO: 10)
d67: SEQ ID NO: 22 (SEQ ID NO: 11)
d77: SEQ ID NO: 23 (SEQ ID NO: 12)
d95: SEQ ID NO: 24 (SEQ ID NO: 13)
FGF18 stimulates the four FGF receptors FGFR1c, FGFR2c, FGFR3c and FGFR4, but the intensity of stimulation varies depending on the receptor. It is believed that summation of stimulations on these receptors results in inhibition of hair growth.
According to teachings disclosed in literature, the FGF receptor genes FGFR1c, FGFR2c, FGFR3c and FGFR4 were respectively introduced by means of genetic engineering into Ba/F3 cell strain (obtained from RIKEN BRC) which is mouse IL-3-dependent proB cell to thereby prepare cells on whose surface FGFR was compulsively expressed (Ornitz, D M., Xu, J., Colvin, J S., McEwen, D G, MacArthur, C A., Coulier, E, Gao, G. and Goldfarb, M., 1996. Receptor specificity of the fibroblast growth factor family. J. Biol. Chem. 271(25):15292-15297; Yoneda, A., Asada, M., Oda, Y, Suzuki, M. and Imamura, T., 2000. Engineering of an FGF-proteoglycan fusion protein with heparin-independent, mitogenic activity. Nat. Biotec. 18(6):641-644).
Using these cells, the antagonist activity of test samples [whether or not test samples inhibit the DNA synthesis of FGF18-stimulated cell (this is taken as 100%)] was examined while stimulating each receptor with 30 ng/ml FGF18, in the co-presence of each partial peptide of FGF18 at 1 μg/ml (
(
(
From
From
By using these partial polypeptides of FGF18 which act as FGF18 activity inhibiting substances, eliminated the hair growth inhibiting effect of FGF18 in the regulation of hair growth can be, thus, achieving promoted hair growth.
Further, partial polypeptides were prepared in the same manner based on SEQ ID NO: 14 which corresponds to the amino acid sequence of mouse FGF18 by deleting amino acids from up to position 25 to up to position 125 counted from the C-terminus Partial peptides dc25-dc125 (indicating the number of amino acids deleted from C-terminal) were prepared.
The amino acid sequences (nucleotide sequences) of individual partial peptides correspond to the following SEQ ID NOS.
dc25: SEQ ID NO: 34 (SEQ ID NO: 25)
dc43: SEQ ID NO: 35 (SEQ ID NO: 26)
dc57: SEQ ID NO: 36 (SEQ ID NO: 27)
dc67: SEQ ID NO: 37 (SEQ ID NO: 28)
dc82: SEQ ID NO: 38 (SEQ ID NO: 29)
dc94: SEQ ID NO: 39 (SEQ ID NO: 30)
dc108: SEQ ID NO: 40 (SEQ ID NO: 31)
dc113: SEQ ID NO: 41 (SEQ ID NO: 32)
dc125: SEQ ID NO: 42 (SEQ ID NO: 33)
All these partial polypeptide of FGF18 except dc25 have an inhibitory effect against FGF18 activity similar to the effect of the above-listed N-terminal deleted partial peptides. Therefore, these partial polypeptide are capable of eliminating the hair growth inhibiting effect of FGF18 and promoting hair growth.
The cell on whose surface FGR4 is compulsively expressed (R4/Ba/F3 cell) prepared above was cultured using various plant extracts as test substances in the presence of FGF18. As a positive control, a commercial FGF18 protein was used. The cell count after culturing for a specific time period was determined with Cell Counting Kit-8 (manufactured by Dojindo Laboratories and sold by Wako Pure Chemical Industries) by measuring the coloring at 450 nm which was proportionate to the yield of WST-8 formazan.
As a result, it was found that Momordica charantia extract acts as an FGF18 activity inhibiting substance and inhibits the growth of FGFR4/BaF3 cell due to FGF18.
To 1.5 g of dried Momordica charantia, 30 ml of distilled water was added and the resultant mixture was boiled for 15 min. The thus obtained extract was filtered with a filter paper. The filtrate was collected to obtain a Momordica charantia hot water extract. To 1.5 g of dried Momordica charantia, 30 ml of 70% ethanol aqueous solution was added, followed by soaking extraction at room temperature for 7 days. The thus obtained extract was filtered with a filter paper to give a 70% ethanol extract of Momordica charantia.
In the same manner as in Example 4, the activity of the FGF18 activity inhibiting substance of the present invention was measured using FGFR4/Ba/F3 cell. Specifically, measurement was performed as described below. Briefly, RPMI1640 medium containing 10% FBS and 1% Antibiotic G-418 Sulfate (Promega; V7983) was added to each well of 96-well cell culture plates (50 μl/well). Subsequently, various concentrations of test solutions prepared by diluting samples with water were added (10 μl/well), followed by addition of 50 μl of cell suspension in which 5×104 R4/Ba/F3 cells were suspended in RPMI1640 medium containing 10% FBS and 1% Antibiotic G-418 Sulfate. The resultant mixture was stirred lightly. Further, 10 μl of heparin/10% FBS/1% Antibiotic G-418 Sulfate/RPMI1640 medium (final heparin concentration: 5 μg/ml) and 10 μl of FGF18 (PeproTech; 100-28) solution (final concentration of FGF18: 3 ng/ml) were added thereto. Then, the cell was cultured in a carbon dioxide incubator at 37° C. under 5% CO2 for 72 hr. To determine the growth of FGFR4/Ba/F3 cell, 10 μl of Cell Counting Kit-8 (manufactured by Dojindo Laboratories and sold by Wako Pure Chemical Industries)/PBS solution was added to each well after 72-hour culture, followed by culturing for another 3 hrs, and the coloring at 450 nm which was proportionate to the yield of WST-8 formazan was measured.
In the measurement, 10 μl of FGF18 (PeproTech; 100-28) solution (final FGF18 concentration: 3 ng/ml) was used as a positive control. As a negative control, 10 μl of water or ethanol solution (final concentration of ethanol was adjusted to 1% or less) used for preparing test solutions was used. Cell growth inhibition rate (%) was calculated by formula (A) described below.
Cell growth inhibition rate(%)=100×{(Absorbance upon addition of FGF18 and water or ethanol solution)−(Absorbance upon addition of FGF18 and test sample)}/Absorbance upon addition of FGF18 and water or ethanol solution−Absorbance upon addition of water or ethanol solution) [Formula (A)]
The results on Momordica charantia hot water extract are shown in
In order to examine the in vivo effect of the FGF18 activity inhibiting substance, a test was performed on C3H/He mice in telogen phase of the hair cycle.
Fifteen ml of distilled water was added to 1.1 g of dried Momordica charantia. The resultant mixture was boiled for 20 min. After the extract returned to room temperature, it was filtered with a filter paper. By adding an equal volume of ethanol to the filtrate, 50% ethanol solution of Momordica charantia hot water extract was prepared. This solution was filtered through a 0.45 μm Millex HV sterilization filter (Millipore). Then, glycerol was added to the filtrate to give a concentration of 1% to thereby prepare a test solution. The thus prepared Momordica charantia hot water extract (containing 49.5% ethanol and 1% glycerol) was applied to the dorsal skin of C3H/He mice in telogen phase of the hair cycle. After anesthetizing, dorsal hair of 7 week-old C3H/He male mice was shaved gently with a hair clipper. After shaving, 200 μl of the Momordica charantia extract as a test solution (containing 49.5% ethanol and 1% glycerol) or water-ethanol-glycerol solution (containing 49.5% water, 49.5% ethanol and 1% glycerol) was applied to the dorsal skin of 5 mice per group (day 0). In a similar manner, application was performed daily for 11 days (excluding day 5 and day 6). The state of hair regrowth in the shaved area in the back of mice was observed with eyes at day 10, day 14, day 17, day 21 and day 24, to thereby give hair regrowth scores. Each state was scored as follows: 1) pigmentation: 1 point; 2) short hair: 2 points; 3) normal hair: 3 points. The ratio of the area of each hair regrowth state to the total shaved area was determined in %. Then, hair regrowth score was calculated by the formula described below. According to this calculation method, the score is 100 when the total shaved area has been recovered to normal hair state.
Hair regrowth score=[ratio of pigmentation area(%)×1+ratio of short hair area(%)×2+ratio of normal hair area(%)×3]/3
The state of hair regrowth in the shaved area in the back of mice was observed at day 10, day 14, day 17, day 21 and day 24 after the first application. As a result, as shown in
As described so far, it was demonstrated that Momordica charantia hot water extract, an FGF18 activity inhibiting substance, has an effect as a hair regrowth promoting agent.
A formulation of a hair shampoo comprising the Momordica charantia hot water extract of the present invention and a method of preparing the shampoo are described below.
A hair shampoo was prepared according to the following formulation and preparation method.
The components listed above were heated to 80° C., mixed by stirring and then cooled under stirring. Thus, the shampoo of the present invention was prepared.
A formulation of a hair liquid comprising a Momordica charantia hot water extract of the present invention and a method of preparing the hair liquid are described below.
A hair liquid was prepared according to the following formulation and preparation method.
The components listed above other than purified water were dissolved by stirring and then purified water was added. Thus, the hair liquid of the present invention was prepared.
A formulation of a hair cream comprising a Momordica charantia hot water extract of the present invention and a method of preparing the hair cream are described below.
A hair cream was prepared according to the following formulation and preparation method.
The components listed above were mixed by stiffing to thereby prepare the hair cream of the present invention.
According to the present invention, effective hair growth promoting agents, hair regrowth promoting agents and therapeutics for alopecia are provided. By incorporating these agents, it is possible to provide shampoos and hair liquids with hair growth promoting activity, as well as pharmaceutical compositions for treating alopecia.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2007-038223 | Feb 2007 | JP | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/JP2008/052781 | 2/19/2008 | WO | 00 | 8/18/2009 |
