Claims
- 1. An isolated haloperoxidase obtained from a Curvularia verruculosa strain.
- 2. A haloperoxidase according to claim 1 which is obtained from Curvularia verruculosa CBS 147.63 or a mutant strain thereof.
- 3. A haloperoxidase according to claim 1 which is obtained from Curvularia verruculosa CBS 444.70 or a mutant strain thereof.
- 4. A haloperoxidase according to claim 1 which retains at least 50% activity after one hour incubation at pH 7.0 and 60.degree. C. in the presence of 0.1% H.sub.2 O.sub.2.
- 5. A haloperoxidase according to claim 1 which retains at least 75% activity after one hour incubation at pH 7.0 and 60.degree. C. in the presence of 0.1% H.sub.2 O.sub.2.
- 6. A haloperoxidase according to claim 1 which retains at least 50% activity at 30.degree. C. over a pH range of between about 4 and about 11.
- 7. A haloperoxidase according to claim 1 which retains at least 80% activity at 30.degree. C. over a pH range of between about 4 and about 11.
- 8. A haloperoxidase according to claim 1 which has a temperature optimum in the range of 50.degree.-70.degree. C.
- 9. A haloperoxidase according to claim 1 which has a temperature optimum in the range of 55.degree.-65.degree. C.
- 10. A haloperoxidase according to claim 9 which has a temperature optimum of about 60.degree. C. at pH 5.5.
- 11. A haloperoxidase according to claim 1 which has a pH optimum in the range of about 5.25 to about 6.25.
- 12. A haloperoxidase according to claim 11 which has a pH optimum of about 5.75 at 30.degree. C.
- 13. A haloperoxidase according to claim 1 which prefers Br.sup.- over Cl.sup.- as a substrate.
- 14. A haloperoxidase according to claim 1 which contains the partial peptide sequence set forth in SEQ ID NO:3.
- 15. A haloperoxidase according to claim 1 which contains the partial peptide sequence set forth in SEQ ID NO:4.
- 16. A haloperoxidase according to claim 1 which contains the partial peptide sequence set forth in SEQ. ID NO:5.
- 17. A haloperoxidase according to claim 1 which contains the partial peptide sequence set forth in SEQ ID NO:6.
- 18. A haloperoxidase according to claim 1 which contains the partial peptide sequence set forth in SEQ ID NO:7.
- 19. A haloperoxidase according to claim 1 which contains the partial peptide sequence set forth in SEQ ID NO:8.
- 20. A haloperoxidase according to claim 1 which contains the partial peptide sequence set forth in SEQ ID NO:9.
- 21. A haloperoxidase according to claim 1 which contains the partial peptide sequence set forth in SEQ ID NO:10.
- 22. A haloperoxidase according to claim 1 which has an amino acid sequence set forth in SEQ ID NO:2.
- 23. An isolated haloperoxidase which is encoded by the coding region of the nucleic acid sequence contained in the plasmid pHAP4A.1 of E. coli DH10B, NRRL B-21519.
- 24. A method for producing the haloperoxidase of claim 1 comprising
- (a) fermenting a Curvularia verruculosa strain to produce a supernatant comprising the haloperoxidase; and
- (b) recovering the haloperoxidase.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of U.S. Ser. No. 08/603,534 filed Jul. 11, 1996 now abandoned, which claims the benefit of U.S. Ser. No. 60/001,194 filed Jul. 14, 1995 now abandoned.
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4707446 |
Geigert et al. |
Nov 1987 |
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4707447 |
Geigert et al. |
Nov 1987 |
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Non-Patent Literature Citations (4)
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Van Schijndel, et al., Biochim. Biophys. Acta, 1161 (1993) 249-256. |
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E.G.M. Vollenbroek et al., "Vanadium Chloroperoxidases Occur Widely in Nature", Biochemical Soc. Trans. 23(2): 267-271, 1995. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
603534 |
Jul 1996 |
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