Claims
- 1. A conjugate having the following structure: ##STR16## wherein a and a' when taken alone are 1 to 4 groups independently selected from the group consisting of: hydrogen, C.sub.1 -C.sub.10 -alkyl, C.sub.1 -C.sub.10 -alkoxy, C.sub.1 -C.sub.10 -alkylthio, halo-C.sub.1 -C.sub.10 -alkyl, C.sub.1 -C.sub.10 -alkylamino, di-(C.sub.1 -C.sub.10 -alkyl)amino, aryl-C.sub.1 -C.sub.10 -alkyl, optionally substituted aryl, halogen, amino, carboxy, carboxamido, hydroxy, mercapto, nitro, nitroso, sulfo, phospho and protected forms thereof; or
- G is selected from S, O, and NR wherein R is hydrogen, C.sub.1 -C.sub.10 -alkyl, optionally substituted aryl, optionally substituted sulfonyl, thiophenyl, carboxy, carboxamido, and protected forms thereof;
- A is a linking moiety of the formula -L-y, wherein y is a functional group that can react directly or after activation with functional groups in a second molecule and L is spacer group consisting of from 1 to about 50 atoms; and
- Q is an oligonucleotide.
- 2. The conjugate according to claim 1 wherein a is hydrogen and a' is amino, halogen, hydroxy, nitro, and protected forms thereof.
- 3. The conjugate according to claim 1 wherein L is C.sub.1 -C.sub.10 -alkyl and y is selected from a group consisting of is chosen from a group consisting of hydroxyl (--OH), thiol (--SH), carboxy (--C(.dbd.O)OH), amino (--NH.sub.2), aldehyde (--CH(.dbd.O)), leaving group, Michael acceptor, phosphoramidite, phosphonate and protected forms of these functional groups.
- 4. The conjugate according to claim 3 wherein y is a phosphoramidite or a phosphonate.
- 5. The conjugate according to claim 4 wherein a is hydrogen and a' is selected from the group consisting of hydrogen, amino, halogen, hydroxy, nitro, and protected forms thereof.
- 6. The conjugate of claim 1, wherein said oligonucleotide is a deoxyribonucleotide from about 10 to about 100 bases in length.
- 7. A method of detecting a target nucleic acid, comprising:
- a. mixing a hapten:oligonucleotide conjugate according to claim 1 with the target nucleic acid under conditions promoting hybridization, said conjugate having an oligonucleotide component, Q, which is complementary to the target nucleic acid;
- b. separating unhybridized conjugate from hybridized conjugate; and
- c. detecting the amount of hybridized hapten:oligonucleotide conjugate;
- wherein at least one of said separating and detecting steps is performed using a specific binding member for said hapten.
- 8. The method according to claim 7 wherein separation is performed using a solid phase to which is immobilized said specific binding member for said hapten.
- 9. The method according to claim 7 wherein detection is performed using a conjugate of a detectable label and said specific binding member for said hapten.
- 10. The method according to claim 7, wherein the target nucleic acid is amplified prior to said separating step.
Parent Case Info
This application is a continuation-in-part of U.S. Ser. No. 07/808,839 filed Dec. 17, 1991, now abandoned, the whole of which is incorporated by reference.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5204374 |
Muller et al. |
Apr 1994 |
|
Non-Patent Literature Citations (1)
Entry |
Lindquist et al. Carcinogenesis 10(12): 2187-95. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
808839 |
Dec 1991 |
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