Patent Application
20150050399
The present invention relates to a heat-stable oil-in-water emulsion, more particularly to a heat-stable oil-in-water emulsion that contains non-modified egg yolk lecithin and water-insoluble cellulosic fibre.
Egg yolk is widely used as an emulsifying agent in the preparation of oil-in-water emulsions such as mayonnaise, dressings and sauces. However, emulsions stabilized by ordinary egg yolk will break up during heating, resulting in oil separation and surface burning. Furthermore, heat treatment induces an undesirable texture change in these emulsions by rendering them more ‘jelly’. Consequently, non-modified egg yolk is generally not used in oil-in-water emulsions that have to be heat-sterilized or that are designed for use in hot applications.
GB 1 525 929 describes water-in-oil emulsions with improved heat-stability which contain an egg yolk that has been modified with phospholipase A. By treating egg yolk with phospholipase A, phosphatides contained therein are hydrolysed to lysophosphatides and fatty acids. Lysophosphatides such as lysophosphatidyl choline are effective oil-in-water emulsifiers capable of stabilising oil-in-water emulsions against heat-induced emulsion break-up.
U.S. Pat. No. 5,314,706 describes a heat-stable oil-in-water emulsion that comprises 3-80 wt. % oil, water and acetic acid and 1-10 wt. % of an emulsification agent comprising a homogenate of egg yolk, of exogenous soybean lysophospholipid containing lysophosphatidylcholine, and of an egg yolk stabilizer substance selected from the group consisting of salt and sucrose.
U.S. Pat. No. 5,773,072 describes a process for preparing a heat-stable oil and water emulsion sauce which comprises:
The aforementioned methods for preparing egg-yolk based heat-stable oil-in-water emulsions have the disadvantage that they require enzymatic modification of the lecithin component or the use of a synthetic emulsifier.
EP-A 0 295 865 describes oil-in-water emulsions comprising a sufficient amount of parenchymal cell cellulose to stabilize the emulsion. Example 4 describes mayonnaise formulations that have not been subjected to heat treatment and that contain:
WO 2010/102920 describes a method for preparing an edible emulsion comprising oil, water and insoluble fibre. Example 1 describes a low fat mayonnaise containing 20 wt. % oil, 2.5 wt. % citrus fibre, 4.0 wt. % enzyme modified egg yolk (containing 10% NaCl), 2.4 wt. % vinegar (12%), 1.5 wt. % salt, 3.0 wt. % sugar, 0.01 wt. % EDTA, 0.1 wt. % K-sorbate and water. The preparation of this emulsion did not comprise any heat treatment.
US 2011/0020525 describes a method of minimizing phase separation in an emulsion, said method comprising:
Non-prepublished patent application WO 2012/046072 A1 discloses a method for making an edible emulsion containing egg yolk and cellulosic fibre from citrus fruit. No mentioning is made of heating the emulsion and heat stability of the emulsion upon sterilization and pasteurization.
U.S. Pat. No. 4,774,099 discloses baked goods containing a shortening, eggs and orange fibre as ingredients. A foodstuff wherein an emulsion is brought into contact with other ingredients (and not mixed) and then heated is not disclosed though. Moreover, an emulsion as such that is heated is not disclosed either.
WO 2005/039316 A1 discloses a salad dressing comprising egg yolk, citrus fibre and water-soluble polysaccharide thickener, pectin. The emulsion is pasteurized for 2 minutes at a temperature between 88 and 90° C. There is no disclosure of the preparation of a composite foodstuff containing a heat-stable emulsion and heating the food stuff. Moreover, there is no mentioning of sterilization of an emulsion.
The present invention enables the preparation of heat-stable oil-in-water emulsions without the use of enzyme modified egg yolk lecithin or synthetic emulsifiers. The inventors have unexpectedly discovered that the limited heat-stability provided by egg yolk can be improved dramatically by the introduction of water-insoluble cellulosic fibre. Thus, the combined use of egg yolk and water-insoluble cellulosic fibre enables the preparation of oil-in-water emulsions that can suitably be heated in an oven at a temperature of 180° C. or more for several minutes without breaking up and/or without developing undesirable textural changes.
Accordingly, one aspect of the invention relates to a process of preparing a food product comprising 1-100 wt. % of a heat-stable oil-in-water emulsion, said process comprising heating the food product to a temperature in excess of 90° C. for at least 5 minutes, wherein the heat-stable emulsion contains:
A weight ratio PC/LPC of more than 2:1 is indicative of the fact that the egg yolk lecithin is substantially non-hydrolyzed. Heat-stabilized egg yolk typically has a PC/LPC ratio of less than 1:10, whereas non-modified egg yolk usually has a PC/LPC ratio of about 6:1.
The heat-stability of the present oil-in-water emulsion further enables the production of the emulsions in sterilized or pasteurized form. Consequently, a further aspect of the invention relates to a sterilized or pasteurized oil-in-water emulsion comprising:
One aspect of the present invention relates to a process of preparing a food product comprising 1-100 wt. % of a heat-stable oil-in-water emulsion, said process comprising heating the food product to a temperature in excess of 90° C. for at least 5 minutes, wherein the heat-stable emulsion contains:
The present process encompasses, for instance, the preparation of a composite foodstuff in which the heat-stable oil-in-water emulsion is combined with a food substrate and wherein the composite foodstuff so obtained is subsequently heated, e.g. under a grill or in an oven. The process also encompasses the preparation of a heat sterilized or heat pasteurized emulsion by subjecting a heat-stable oil-in-water emulsion to heat sterilization or heat pasteurization.
The term “oil” as used herein refers to lipids selected from triglycerides, diglycerides, monoglycerides and combinations thereof. The oil may be solid or liquid at ambient temperature.
The term “lecithin” as used herein refers to phospholipids selected from the group of phosphatidyl choline, phosphatidyl ethanolamine, lysophosphatidyl choline, lysophosphatidyl ethanolamine, sphingomyelin and combinations thereof.
Cellulose is found in plants as microfibrils, which typically have a diameter of 2-20 nm and a length of 100-40,000 nm. These microfibrils form the structurally strong framework in the cell walls of plant materials. Cellulose is a linear polymer of β-(1→4)-D-glucopyranose units. Cellulose molecules typically consist of 2,000-14,000 of such units and are completely insoluble in normal aqueous solutions. When dispersed in an aqueous solution insoluble cellulosic fibres typically bind considerable amounts of water. Cellulosic fibres may contain other fibrous components such as hemicelluloses, pectins and lignin.
Purified cellulose is used as a starting material for the manufacture of a number of water-soluble hydrocolloids, such as carboxymethyl cellulose (CMC). The manufacture of these cellulose derivatives involves chemical modification of the natural cellulose material. CMC, for instance, is synthesized by the alkali-catalyzed reaction of cellulose with chloroacetic acid. The polar (organic acid) carboxyl groups render the cellulose soluble and chemically reactive.
Typically, the water-insoluble cellulosic fibres of the present invention are substantially or completely underivatised. According to a particularly preferred embodiment of the invention the water-insoluble cellulosic fibres are natural cellulosic fibres which have not been chemically modified.
Unlike, for instance, microcrystalline cellulose, the cellulose molecules within the present water-insoluble cellulosic fibres are essentially non-hydrolysed. Typically, the cellulose molecules contained within the water-insoluble cellulosic fibres employed in accordance with the present invention contain at least 1,000, preferably at least 2,000 β-(l→4)-D-glucopyranose units.
It was found that water-insoluble cellulosic fibres originating from fruit yield particularly satisfactory results. According to a particularly preferred embodiment the water-insoluble cellulosic fibres employed originate from citrus fruit, tomatoes, peaches, pears, apples, plums or combinations thereof. Even more preferably, the insoluble cellulosic fibres are water-insoluble citrus fibres. Most preferably, the water-insoluble citrus fibres originate from the albedo and/or the flavedo of citrus fruits.
The water-insoluble cellulosic fibres employed in the oil-in-water emulsion of the present invention typically have a length of 1-200 μm. Preferably, the cellulosic fibres have an average length of 5-100 μm, most preferably of 10-80 μm.
The water-insoluble cellulosic fibre is preferably contained in the heat-stable oil-in-water emulsion in a concentration of 0.2-12%, more preferably of 0.5-10% and most preferably of 0.8-8% by weight of water. Expressed differently, the heat-stable emulsion preferably contains 0.4-4.5%, most preferably 0.6-4.0% of the water-insoluble cellulosic fibre by weight of the total emulsion.
In the present process the food product comprising the heat-stable oil-in-water emulsion is typically heated by introducing it into an oven or a grill which is kept at a temperature of at least 150° C., more preferably of 180-300° C. Typically, the food product comprising the emulsion is heated under these conditions for at least 5 minutes, more preferably of at least 8 minutes and most preferably 10-50 minutes.
Examples of oil-in-water emulsions encompassed by the present invention include mayonnaise, dressings and sauces. Preferably, the oil-in-water emulsion is a mayonnaise or a sauce, most preferably a mayonnaise.
According to another preferred embodiment, the heat-stable emulsion has a pH of 3.0-4.5, most preferably a pH of 3.3-3.7. Even more preferably, the emulsion has been acidified with a food grade acid. Most preferably, the emulsion contains 0.05-1.0 wt. % of a food acid selected from acetic acid, citric acid, lactic acid, phosphoric acid and combinations thereof.
Egg yolk is a good source of phospholipids. Typically, phospholipids represent approximately 10% of the wet weight of egg yolk, which is equivalent to about 27% of the total egg yolk lipids. The main components of egg-yolk lecithin are phosphatidylcholine (PC, 80%) and phosphatidylethanolamine (PE, 12%). Egg-yolk lecithin also contains lysophosphatidylcholine (LPC), sphingomyelin (SM), and neutral lipids in minor quantities.
The present oil-in-water emulsion preferably contains 0.15-0.9 wt. %, more preferably 0.2-0.85 wt. % and most preferably 0.25-0.8 wt. % of egg yolk lecithin.
As explained herein before, the present invention offers the advantage that it achieves heat-stability without enzymatic modification of the egg yolk contained therein. Accordingly, the heat-stable emulsion advantageously contains 0.2-5 wt. %, more preferably 0.4-4.5 wt, % and most preferably 0.5-4 wt. % of non-modified egg yolk solids.
The weight ratio PC/LPC of the egg yolk lecithin comprised in the heat-stable emulsion preferably exceeds 3:1, most preferably it exceeds 4:1.
The dispersed oil phase of the present oil-in-water emulsion typically has a volume weighted geometric mean diameter (D3,3) in the range of 0.3-15 μm, most preferably of 0.5-10 μm. This mean diameter may suitably be determined using the method described by Goudappel et al. (Measurement of Oil Droplet Size Distributions in Food Oil/Water Emulsions by Time Domain Pulsed Field Gradient NMR, Journal of Colloid and Interface Science 239, 535-542 (2001)). This article describes a method for measuring oil droplet size distributions by means of a benchtop pulsed field gradient NMR spectrometer operating in the time domain. The continuous water phase is successfully suppressed by gradient pulses in order to measure the dispersed oil phase. The article also explains how the mean diameter D3,3 of the dispersed oil phase of an oil-in-water emulsion may be determined.
The present invention offers the advantage that it enables the manufacture of a heat-stable emulsion without the use of water-soluble polysaccharide thickeners. Consequently, in a particularly preferred embodiment the present emulsion contains no water-soluble polysaccharide thickener.
In accordance with one advantageous embodiment of the present process the food product is a composite foodstuff comprising a food substrate and the heat-stable oil-in-water emulsion in a weight ratio of 80:20 to 99:1. The food substrate may be selected from, for example, meat, fish, shellfish, shrimps, pies, vegetables and combinations thereof.
The composite foodstuff is suitably prepared by applying the oil-in-water emulsion onto the food substrate before the heating, e.g. by pouring the emulsion over the food substrate or by spreading it onto said substrate. Hence for the purpose of the present invention the composite foodstuff comprises a heat-stable emulsion which still is in the form of an emulsion. The emulsion suitably is not mixed with the food substrate, preferably it is only brought into contact with the food substrate.
The composite food product is preferably heated by grilling or by heating in an oven or a microwave. Most preferably, the composite food product is heated by grilling or in an oven.
In accordance with another advantageous embodiment, the food product is heated to sterilize or pasteurize the emulsion. Most preferably, the food product is pasteurized or sterilized by heating the food product to a temperature of at least 95° C., most preferably of at least 100° C.
According to a particularly preferred embodiment, the food product is selected from a mayonnaise, a sauce, a salad, a dressing and a whole meal. Hence for the purpose of the present invention preferably the food product is a mayonnaise or dressing or a sauce which is heated as such. This distinguishes from the composite food stuff in that the emulsion is not brought into contact with a food substrate before the heating. Most preferably, the food product is a mayonnaise.
Preferably, the mayonnaise comprises 10-85 wt. % oil, 0.2-5 wt. % egg yolk solids and at least 5 wt. % of an acidifier selected from vinegar, lemon juice and a combination thereof. The mayonnaise may suitably contain further ingredients, such as herbs, spices and mustard.
Hence preferably the present invention relates to a process of preparing a food product, said food product being a composite foodstuff comprising a food substrate and the heat-stable oil-in-water emulsion in a weight ratio of 80:20 to 99:1 or a foodstuff selected from the group consisting of a mayonnaise, a dressing and a sauce, said process comprising heating the food product to a temperature in excess of 90° C. for at least 5 minutes, wherein the heat-stable emulsion contains:
Another aspect of the invention relates to the use of a heat-stable oil-in-water emulsion as defined herein before for preparing a composite foodstuff, said use comprising heating a combination of a food substrate and the heat-stable emulsion to a temperature in excess of 100° C. for at least 5 minutes.
Yet another aspect of the present invention relates to a sterilized or pasteurized oil-in-water emulsion, said oil-in-water emulsion having a pH of 3.0-4.5 and comprising:
According to a particularly preferred embodiment the sterilized or pasteurized oil-in-water emulsion is a pasteurized or sterilized heat-stable oil-in-water emulsion as defined herein before. Most preferably, the emulsion is a pasteurised emulsion.
Preferably, the sterilized or pasteurized oil-in-water emulsion is selected from the group of a mayonnaise, a dressing and a sauce. Most preferably, the emulsion is a mayonnaise. According to an especially preferred embodiment, the mayonnaise is packaged in a container that carries information indicating that the emulsion can be used in hot applications, notably hot applications that involve heating of the emulsion to a temperature in excess of 90° C.
The invention is further illustrated by the following non-limiting examples.
Mayonnaises were produced on the basis of the recipes shown in Table 1.
1 Liquid egg yolk, from Bouwhuis Enthoven, the Netherlands (PC/LPC ratio of 6:1)
2 Stabilised egg yolk (enzyme modified), from Bouwhuis Enthoven, the Netherlands (PC/LPC ratio of 1:10)
3 Citrus Fibre AQ Plus Type N, from Herbafood
Mayonnaise A was prepared using the following procedure:
Mayonnaises 1 and B were prepared using the following procedure:
The heat stability of the mayonnaises described in Example 1 was assessed by subjecting the products to the following test procedure:
The spreading test showed that heat treated mayonnaise A suffered from oil separation. Furthermore, the rheology of mayonnaise A had changed as a result of the heat treatment in that it had become more ‘jelly’ and in that it tended to fracture upon compression.
The results of the oil droplet size measurements are shown in Table 2 (D3,3 is the volume weighted average oil droplet size).
CSLM showed that the heat treatment had caused substantial oil droplet aggregation in mayonnaise A. The CSLM images of mayonnaises 1 and B after heat treatment were very similar to those of the same mayonnaises before the heat treatment.
The heat stability of the mayonnaises described in Example 1 was assessed by subjecting them to the following grilling test.
After the grilling mayonnaise A showed signs of oil exudation. Furthermore, mayonnaise A was found to have developed a pudding-like texture. Mayonnaises 1 and B did not show any signs of oil exudation and the texture after grilling was similar to the texture of the same product before grilling.
The results of the oil droplet size measurements are shown in Table 3 (D3,3 is the volume weighted average oil droplet size)
The mayonnaises described in Example 1 were sterilized by heating them to 121° C. for 15 minutes. Oil droplet size was measured before and after sterilization. The results are shown in Table 4.
| Number | Date | Country | Kind |
|---|---|---|---|
| 12159619.1 | Mar 2012 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2013/053058 | 2/15/2013 | WO | 00 |
