Patent Application
20190062339
The present invention relates to hemi-synthetic trilobine analogues useful as DNA methyltransferase (DNMT) inhibitors, notably in the treatment of cancer and in neurological diseases.
Epigenetic modifications play an essential role in the establishment and regulation of differentiation programs that define when and where genes are expressed. Among these modifications, C5 methylation of deoxycytidines (dC) in the DNA was shown to play a key role in the epigenetic regulation in mammals. It is the most stable epigenetic mark and occurs at position 5 of the cytosine ring within mainly CpG dinucleotides. The CpG sites are not distributed randomly across the human genome: they can be regrouped in islands, which are essentially located in promoters, present as repeated sequences, or located in CpG island shores. Hypermethylation of CpG islands located in promoters induces gene silencing while hypomethylation induces gene expression. Most of the CpG sites in the repetitive sequences are methylated, thus preventing chromosomal instability by silencing transposable DNA elements. Furthermore, some CpG islands located in promoters become methylated in a sustainable manner during development, which results in turning gene expression off, a phenomenon observed in X-chromosome inactivation, imprinted genes and developmental genes. Methylation status of the promoters regulates dynamically gene expression through modification of the recognition by transcription factors and the proteins involved in chromatin remodeling.
DNA methyltransferases (DNMTs) catalyse the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to position 5 of the cytosine at the CpG site. Two families of catalytically active DNMTs have been identified: DNMT1, responsible for DNA methylation maintenance during replication, and DNMT3A and 3B, responsible for de novo DNA methylation.
Alterations of DNA methylation patterns lead to various diseases such as cancer and neurological diseases. Cancerous cells present aberrant DNA methylation. In particular, a specific hypermethylation of tumour suppressor genes is observed, inducing their silencing. Restoring their expression by specific inhibition of DNA methylation represents an attractive therapeutic strategy. In addition, it has been shown that demethylating agents prime cancer cells towards immunotherapy and sensitize them towards chemotherapy.
DNMT inhibitors can be divided into two families: nucleoside analogues and non-nucleosides. The first are the most active ones. Two of them are FDA approved: 5-azacytidine (Vidaza®) and 5-aza-2′-deoxycytidine (Dacogene™). Despite their efficiency, poor bioavailability, instability in physiologic media and little selectivity restrict their use. Non-nucleoside analogues present various structures and mechanisms of action. Many of them were shown to target the catalytic site but suffer from lack of specificity and weak activity.
There exists thus a need for novel DNMT inhibitors.
The inventors of the present invention have thus discovered that hemisynthetic bisbenzylisoquinoline alkaloids can be used as DNMT inhibitors. Indeed, in their search of new DNMT inhibitors, they discovered that in the natural extracts of Cocculus hirsutus were present potent inhibitors of DNA methylation able to express, in leukaemia KG-1 cells, the luciferase gene under the control of a methylated CMV promoter upon demethylation of the promoter and chromatin decompaction. By a phytochemical approach, the natural bisbenzylisoquinoline alkaloid trilobine and natural analogues were thus identified. The trilobine scaffold was chemically modulated, and the ability of the derivatives to inhibit the DNMTs, to demethylate gene promoters and to induce chromatin opening and gene reexpression, such as of tumour suppressor gene in cancer cells, was characterized.
The present invention concerns thus a compound of the following formula (I), preferably of the following formula (Ia), or a pharmaceutically acceptable salt or solvate thereof:
All stereoisomers, including optical isomers, of formula (I) belong to the invention, as well as the racemic forms.
The term “stereoisomers” used in this invention refers to configurational stereoisomers and includes geometric isomers and optical isomers.
The geometric isomers, also called E/Z isomers or cis-trans isomers, result from the different position of substituents on a double C═C bond which can have a Z or E configuration, also called cis or trans configuration.
The optical isomers result from the different position in space of substituents on an atom (such as a carbon atom) comprising four different substituents. This atom thus represents a chiral or asymmetric center. Optical isomers which are not mirror images of one another are thus designated as “diastereoisomers,” and optical isomers which are non-superimposable mirror images are designated as “enantiomers”.
An equimolar mixture of two enantiomers of a chiral compound is designated as racemate or racemic mixture.
The expression “(C1-C10)-alkyl” in the present invention means a linear or branched saturated hydrocarbon chain comprising from 1 to 10 carbon atoms. Examples of alkyl groups covered by the scope of the present invention are methyl, ethyl, propyl, butyl, tert-butyl, isopropyl, decyl groups, etc.
The expression “(C1-C6)-alkyl” in the present invention means a linear or branched saturated hydrocarbon chain comprising from 1 to 6 carbon atoms. Examples of alkyl groups covered by the scope of the present invention are methyl, ethyl, propyl, butyl, tert-butyl, isopropyl groups, etc.
The expression “(C3-C12)-cycloalkyl” means a saturated hydrocarbon ring comprising from 3 to 12 carbon atoms. Examples of cycloalkyl groups covered by the scope of the present invention are cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, etc.
The expression “(C2-C10)-alkenyl” in the present invention means a linear or branched hydrocarbon chain comprising from 2 to 10 carbon atoms containing at least one double bond, like vinyl and allyl groups.
The term “(C1-C6)alkylamino”, as used in the present invention, refers to an —NHAlk group with Alk representing a (C1-C6)alkyl group as defined above, including, but not limited to, methylamino, ethylamino, n-propylamino, iso-propylamino, n-butylamino, iso-butylamino, sec-butylamino, t-butylamino, n-pentylamino, n-hexylamino, and the like.
The term “di(C1-C6)alkylamino”, as used in the present invention, refers to an —NAlk1Alk2 group with Alk1 and Alk2 representing, independently of one another, a (C1-C6)alkyl group as defined above, including, but not limited to, dimethylamino, diethylamino, ethylmethylamino and the like.
The expression “aryl” in the present invention means a cyclic (mono- or polycyclic) aromatic hydrocarbon group comprising between 5 and 12 carbon atoms, preferably between 6 and 10 carbon atoms. Examples of aryl groups covered by the scope of the present invention are phenyl, naphtyl, etc.
The term “(C1-C10)-alkyl-(C5-C10)-aryl”, as used in the present invention, refers to a (C1-C10)-alkyl group as defined above bound to the molecule via an aryl group as defined above. In particular, it can be a tolyl group (CH3Ph).
The expression “(5-12)-membered-heterocycle group” in the present invention means saturated, unsaturated or aromatic hydrocarbon monocycle or bicycle (comprising fused, bridged or spiro rings, notably fused rings) each cycle having 5 or 6 members and 1 to 4, notably 1 or 2, carbon atoms which have been replaced with heteroatoms, such as nitrogen, oxygen or sulphur. Examples of heteroaryl groups covered by the scope of the present invention are pyridine, thiazole, imidazole, pyrazole, quinoline, indole, pyridazine, quinoxaline, dihydrobenzofuran, purine, furan, pyrrole, pyrroline (such as 3-pyrroline), thiophene, isothiazole, oxadiazole, imidazole, oxazole, isoxazole, pyridine, pyrimidine, pyridazine, pyrazine, triazine, quinoline, isoquinoline, quinoxaline, quinazoline, piperidine, piperazine, triazinane, morpholine, pyrrolidine, dihydropyridines, dihydropyrimidines (notably 1,2-dihydropyrimidine), dihydropyridazines, dihydropyrazines, dihydrotriazines, tetrahydropyridines, tetrahydropyrimidines, tetrahydropyridazines, tetrahydropyrazines, tetrahydrotriazines, benzofurane, benzothiophene, indoline, indolizine, benzothiazole, benzothionyle, benzopyranne, benzoxazole, benzo[1,3]dioxole, benzoisoxazole, benzimidazole, chromane, chromene, dihydrobenzofurane, dihydrobenzothiényle, dihydroisoxazole, isoquinoline, dihydrobenzo[1,4]dioxine, imidazo[1,2-a]pyridine, furo[2,3-c]pyridine, 2,3-dihydro-1H-indene, [1,3]dioxolo[4,5-c]pyridine, pyrrolo[1,2-c]pyrimidine, pyrrolo[1,2-a]pyrimidine, tetrahydronaphtalne, benzo[b][1,4]oxazine, etc.
The term “heteroaryl” as used in the present invention refers to an aromatic heterocycle as defined above. It can be more particularly an aromatic monocyclic or bicyclic heterocycle, each cycle comprising 5 or 6 members, such as a pyrrole, a furan, a thiophene, a thiazole, an isothiazole, an oxazole, an isoxazole, an imidazole, a pyrazole, a pyridine, a pyrimidine, a pyridazine, a pyrazine, an indole, a benzofurane, a benzothiophene, a benzothiazole, a benzoxazole, a benzimidazole, a purine, a quinoline, an isoquinoline, a quinazoline or a quinoxaline.
The term “(C1-C10)-alkyl-(5-12)-membered-heterocycle”, as used in the present invention, refers to a (C1-C10)-alkyl group as defined above bound to the molecule via a (5-12)-membered-heterocycle as defined above.
The expression “halogen” in the present invention means one of the following atoms: fluorine, chlorine, bromine or iodine.
For the purpose of the invention, the term “pharmaceutically acceptable” is intended to mean what is useful to the preparation of a pharmaceutical composition, and what is generally safe and non toxic, for a pharmaceutical use.
The term “pharmaceutically acceptable salt or solvate” is intended to mean, in the framework of the present invention, a salt or solvate of a compound which is pharmaceutically acceptable, as defined above, and which possesses the pharmacological activity of the corresponding compound.
The expression “pharmaceutically acceptable salts” in the present invention, means all the pharmaceutically acceptable salts of the compounds according to the invention are included within the scope of the invention, in particular the salts of weak acids and of weak bases.
The pharmaceutically acceptable salts comprise:
(1) acid addition salts formed with inorganic acids such as hydrochloric, hydrobromic, sulfuric, nitric and phosphoric acid and the like; or formed with organic acids such as acetic, benzenesulfonic, fumaric, glucoheptonic, gluconic, glutamic, glycolic, hydroxynaphtoic, 2-hydroxyethanesulfonic, lactic, maleic, malic, mandelic, methanesulfonic, muconic, 2-naphtalenesulfonic, propionic, succinic, dibenzoyl-L-tartaric, tartaric, p-toluenesulfonic, trimethylacetic, and trifluoroacetic acid and the like, and
(2) salts formed when an acid proton present in the compound is either replaced by a metal ion, such as an alkali metal ion, an alkaline-earth metal ion, or an aluminium ion; or coordinated with an organic or inorganic base. Acceptable organic bases comprise diethanolamine, ethanolamine, N-methylglucamine, triethanolamine, tromethamine and the like. Acceptable inorganic bases comprise aluminium hydroxide, calcium hydroxide, potassium hydroxide, sodium carbonate and sodium hydroxide.
In particular, it can be the hydrochloride, hydrobromide and trifluoroacetate salts.
Acceptable solvates for the therapeutic use of the compounds of the present invention include conventional solvates such as those formed during the last step of the preparation of the compounds of the invention due to the presence of solvents.
It can be for example an hydrate or an alcoholate such as an ethanolate.
The expression “pharmaceutical composition” in the present invention means any composition comprising an effective dose of a compound of the invention and at least one pharmaceutically acceptable excipient. Said excipients are selected, depending on the pharmaceutical form and the desired method of administration, from the usual excipients known by a person skilled in the art.
In one embodiment, the present invention concerns a compound of formula (Ia) or a pharmaceutically acceptable salt or solvate thereof, wherein:
In one embodiment, the compound according to the invention is a compound of formula (I), with the proviso that:
The compounds deleted with the above mentioned provisos are disclosed in the following documents:
According to a particular embodiment, R1 is hydrogen, (C1-C6)-alkyl, or SOOR9.
According to another particular embodiment, R1 is hydrogen, methyl, or SOOCF3.
According to yet another particular embodiment, R1 is (C1-C6)-alkyl, preferably methyl.
According to a particular embodiment, X1 and X2 are, independently of each other, H, (C1-C10)-alkyl, (C1-C10)-alkyl-R2, CONR3R4, CSNR3R4, COR3, COOR6, CN or SOOR7.
According to another particular embodiment, X1 and X2 are, independently of each other, H, (C1-C6)-alkyl, (C1-C6)-alkyl-R2, CONR3R4, CSNR3R4, COR3, COOR6, CN or SOOR7.
According to yet another particular embodiment, at least one of X1 and X2 is not H or (C1-C10)-alkyl, notably X2 is not H or (C1-C10)-alkyl.
According to still another particular embodiment, X1 and X2 are, independently of each other, H, (C1-C10)-alkyl, (C1-C10)-alkyl-R2, CONR3R4, CSNR3R4, COR3, COOR6, CN or SOOR7, preferably H, (C1-C6)-alkyl, (C1-C6)-alkyl-R2, CONR3R4, CSNR3R4, COR3, COOR6, CN or SOOR7, with the proviso that at least one of X1 and X2 is not H or (C1-C10)-alkyl, notably X2 is not H or (C1-C10)-alkyl.
In a preferred embodiment, at least one of X1 and X2, preferably X2, is CONR3R4 or CSNR3R4, preferably CONR3R4.
Advantageously, R2 is CN, NR4R5 or a (5-12)-membered-heterocycle optionally substituted by at least one group selected from NR4R5, OR5, (C1-C10)-alkyl, halogen and oxo (═O), preferably by NR4R5.
More advantageously, R2 is CN or a (5-12)-membered-heterocycle optionally substituted by at least one group selected from NR4R5, OR5, (C1-C6)-alkyl, halogen and oxo (═O), preferably by NR4R5. In this case, the (5-12)-membered-heterocycle can be more particularly a monocyclic or bicyclic heteroaryl containing 1 to 5, notably 1 to 4 nitrogen atoms, each cycle comprising 5 or 6 members, such as a pyrrole, an imidazole, a pyrazole, a pyridine, a pyrimidine, a pyridazine, a pyrazine, an indole, a benzimidazole, a purine, a quinoline, an isoquinoline, a cinnoline, a quinazoline or a quinoxaline.
Even more particularly, R2 is CN or a purine optionally substituted by at least one group selected from NR4R5, OR5, (C1-C10)-alkyl and halogen, preferably by NR4R5, such as by NH2.
In particular, R3 is H, or a group chosen among (C1-C10)-alkyl, (C3-C12)-cycloalkyl, (C2-C10)-alkenyl and (C6-C10)-aryl, said group being optionally substituted by at least one group selected from halogen, NR4R5, mono-(C1-C6)-alkyl-amino, di-(C1-C6)-alkyl-amino, OR4 and (C1-C10)-alkyl-R8, such as selected from NR4R5, di-(C1-C6)-alkyl-amino and (C1-C6)-alkyl-R8.
More particularly, R3 is H, or a group chosen among (C1-C6)-alkyl, (C3-C12)-cycloalkyl, and (C2-C10)-alkenyl, said group being optionally substituted by at least one group selected from halogen, NR4R5, mono-(C1-C6)-alkyl-amino, di-(C1-C6)-alkyl-amino, OR4 and (C1-C6)-alkyl-R8, such as selected from NR4R5, di-(C1-C6)-alkyl-amino and (C1-C6)-alkyl-R8.
Even more particularly, R3 is H, or a group chosen among methyl, ethyl, propyl, cyclohexyl, vinyl and propenyl, said group being optionally substituted by at least one group selected from halogen, NR4R5, mono-(C1-C6)-alkyl-amino, di-(C1-C6)-alkyl-amino, OR4 and (C1-C6)-alkyl-R8, such as selected from NR4R5, di-(C1-C6)-alkyl-amino and (C1-C6)-alkyl-R8.
Yet even more particularly, R3 is H, methyl, chloro-ethyl, diethylaminopropyl, (2,5-dioxo-3-pyrroline)-methyl-cyclohexyl, vinyl or dimethylaminopropenyl.
R4 can be H or (C1-C6)-alkyl, in particular R4 is H, methyl or ethyl, preferably R4 is H.
Preferably, R5 is H or (C1-C10)-alkyl, in particular, R5 is H or (C1-C6)-alkyl, and can be H, methyl, or ethyl.
In particular, R6 is (C1-C10)-alkyl-R2, such as (C1-C6)-alkyl-R2, for example (CH2)m—R2 with m=1-4, such as propyl-R2 or butyl-R2.
Advantageously, R7 is (C1-C10)-alkyl, such as (C1-C6)-alkyl, and can be methyl.
Preferably, R5 is a (5-12)-membered-heterocycle, optionally substituted by at least one group selected from NR4R5, OR5, (C1-C10)-alkyl, halogen and oxo, such as by at least one oxo.
In this case, the (5-12)-membered-heterocycle can be a saturated, unsaturated or aromatic hydrocarbon monocycle or bicycle, each cycle having 5 or 6 members and 1 to 4, notably 1 or 2, carbon atoms which have been replaced with heteroatoms selected from nitrogen, oxygen and sulfur atoms, such as nitrogen atom. In particular, the heterocycle can be a pyrroline, such as a 3-pyrroline. For example, R8 can be 2,5-dioxo-3-pyrroline.
In a first embodiment, X1 and X2 are, independently of each other, H, (C1-C10)-alkyl, (C1-C10)-alkyl-R2, CONR3R4, CSNR3R4, COR3, COOR6, CN, or SOOR7,
wherein:
R2 is CN or a (5-12)-membered-heterocycle, optionally substituted by at least one NR4R5, OR5, (C1-C10)-alkyl and halogen, preferably by NR4R5,
R3 is H, or a group chosen among (C1-C10)-alkyl, (C3-C12)-cycloalkyl and (C2-C10)-alkenyl, said group being optionally substituted by at least one group selected from halogen, NR4R5, mono-(C1-C6)-alkyl-amino, di-(C1-C6)-alkyl-amino, OR4 and (C1-C10)-alkyl-R8, preferably by halogen, NR4R5, di-(C1-C6)-alkyl-amino and (C1-C10)-alkyl-R8,
R4 is H or (C1-C10)-alkyl,
R5 is H or (C1-C10)-alkyl,
R6 is (C1-C10)-alkyl-R2,
R7 is (C1-C10)-alkyl, and
R8 is a (5-12)-membered-heterocycle, substituted by at least one group selected from NR4R5, OR5, (C1-C10)-alkyl, halogen and oxo, preferably by at least one oxo group.
In a second embodiment, X1 and X2 are, independently of each other, H, (C1-C10)-alkyl, (C1-C10)-alkyl-R2, CONR3R4, CSNR3R4, COR3, COOR6, CN, or SOOR7, and at least one of X1 and X2 is not H or (C1-C10)-alkyl, preferably X2 is not H or (C1-C10)-alkyl,
wherein:
R2 is CN or a (5-12)-membered-heterocycle, optionally substituted by at least one NR4R5, OR5, (C1-C10)-alkyl and halogen, preferably by NR4R5,
R3 is H, or a group chosen among (C1-C10)-alkyl, (C3-C12)-cycloalkyl and (C2-C10)-alkenyl, said group being optionally substituted by at least one group selected from halogen, NR4R5, mono-(C1-C6)-alkyl-amino, di-(C1-C6)-alkyl-amino, OR4 and (C1-C10)-alkyl-R8, preferably by halogen, NR4R5, di-(C1-C6)-alkyl-amino and (C1-C10)-alkyl-R8,
R4 is H or (C1-C10)-alkyl,
R5 is H or (C1-C10)-alkyl,
R6 is (C1-C10)-alkyl-R2,
R7 is (C1-C10)-alkyl, and
R8 is a (5-12)-membered-heterocycle, substituted by at least one group selected from NR4R5, OR5, (C1-C10)-alkyl, halogen and oxo, preferably by at least one oxo group,
with the proviso that X2 is not H or (C1-C10)-alkyl.
In a third embodiment, X1 is H, (C1-C10)-alkyl, (C1-C10)-alkyl-R2, CONR3R4, CSNR3R4, COR3, COOR6, CN, or SOOR7, and X2, is CONR3R4 or CSNR3R4, preferably CONR3R4,
wherein:
R2 is CN or a (5-12)-membered-heterocycle, optionally substituted by at least one NR4R5, OR5, (C1-C10)-alkyl and halogen, preferably by NR4R5,
R3 is H, or a group chosen among (C1-C10)-alkyl, (C3-C12)-cycloalkyl and (C2-C10)-alkenyl, said group being optionally substituted by at least one group selected from halogen, NR4R5, mono-(C1-C6)-alkyl-amino, di-(C1-C6)-alkyl-amino, OR4 and (C1-C10)-alkyl-R8, preferably by halogen, NR4R5, di-(C1-C6)-alkyl-amino and (C1-C10)-alkyl-R8,
R4 is H or (C1-C10)-alkyl,
R5 is H or (C1-C10)-alkyl,
R6 is (C1-C10)-alkyl-R2,
R7 is (C1-C10)-alkyl, and
R8 is a (5-12)-membered-heterocycle, substituted by at least one group selected from NR4R5, OR5, (C1-C10)-alkyl, halogen and oxo, preferably by at least one oxo group.
In the above first, second and third above mentioned embodiments, the (5-12)-membered-heterocycle is preferably:
According to another aspect of the present invention, the new hemi-synthetic bis(benzylisoquinoline) analogues of formula I correspond to those of formula I wherein:
According to yet another aspect of the present invention, the new hemi-synthetic bis(benzylisoquinoline) analogues of formula I correspond to those of formula I wherein:
The compounds of the present invention may be selected from the compounds 10-22, 24-28, 30-33, 41-51 and 53-54 and the pharmaceutically acceptable salts and solvates thereof.
The present invention relates also to a compound of formula (I) such as defined above, for use as a drug, notably intented for the treatment of cancer and of neurological diseases, in particular cancer, or intented for cell reprogramming, notably for the treatment of cancer.
In one embodiment, the compounds of formula (I) such as defined above are DNA methyl transferase (DNMT) inhibitors.
Consequently, the present invention relates also to a compound of formula (I) such as defined above, for use as a DNMT inhibitor.
The present invention relates also to a compound of formula (I) such as defined above, for use as a drug intented for inhibiting DNMT.
According to the invention, the expression “DNMT inhibitor” refers to molecules that are able to reduce the DNMT activity. Preferentially, the use of a DNMT inhibitor according to the invention makes it possible to inhibit the activity of said DNMT. The compounds showed also their properties of inhibitors of DNA methylation in cells. The DNMT inhibitory activity can be tested by measuring the expression of a CMV-Luc gene, notably as described in example II.3 of the present application, or by measuring the inhibition of hDNMT3A or hDNMT1 enzymes, notably as described in example II.1 and II.2 of the present application. DNMT inhibitors according to the invention are thus able to reprogramming cells towards less aggressive state.
Also, the present invention relates to a compound of formula (I) such as defined above, for use as a drug, as a DNMT inhibitor for use in a drug for treatment of cancer and neurological diseases, and cell reprogramming, notably intended for the treatment of cancer.
The present invention also relates to the use of a compound of formula (I) such as defined above, for the manufacture of a drug, notably intended for the treatment of cancer.
The present invention also relates to the use of a compound of formula (I) such as defined above, for the manufacture of a drug intended for inhibiting DNMT.
The present invention also relates to a method for the treatment of cancer, comprising the administration to a person in need thereof of an effective dose of a compound of formula (I) such as defined above.
In particular the compound relates to its use in combination to prime cells towards chemotherapy or immunotherapy.
The present invention also relates to a method for inhibiting DNMT, comprising the administration to a person in need thereof of an effective dose of a compound of formula (I) such as defined above.
The neurological disease according to the invention may be more particularly schizophrenia and neurodegenerative diseases.
The cancer according to the invention may be colon cancer; hepatocarcinoma; melanoma; breast cancer; ovarian cancer; kidney cancer; liver cancer; pancreatic cancer; prostate cancer; glioblastoma; lung cancer, such as non-small cell lung cancer; neuroblastoma; myofibroblastic tumor; lymphoma, such as B- and T-cell lymphoma or anaplastic large-cell lymphoma; leukemia, such as AML (acute myeloid leukemia), MDS (myelodysplastic syndrome), CMML (chronic myelomonocytic leukemia) and CML (chronic myeloid leukemia); and multiple myeloma.
The cancer may be more particularly colon cancer, hepatocarcinoma, melanoma, breast cancer, ovarian cancer, kidney cancer, liver cancer, pancreatic cancer, prostate cancer, glioblastoma, non-small cell lung cancer, neuroblastoma, myofibroblastic tumor, B- and T-cell lymphoma or anaplastic large-cell lymphoma, leukemia (AML, MDS, CMML, CML), multiple myeloma. The cancer can be in particular a DNMT-overexpressing cancer.
The present invention also relates to a pharmaceutical composition comprising at least one compound of formula (I) such as defined above, and at least one pharmaceutically acceptable excipient.
The pharmaceutical compositions according to the invention may be formulated notably for oral administration, topical administration or for injection, wherein said compositions are intended for mammals, including humans.
The active ingredient may be administered in unit dosage forms of administration, in mixture with standard pharmaceutical carriers, to animals or to humans. The compounds of the invention as active ingredients may be used in doses ranging between 0.01 mg and 1000 mg per day, given in a single dose once per day or administered in several doses throughout the day, for example twice a day in equal doses. It may be necessary to use doses outside these ranges as determined by the person skilled in the art.
The active ingredient may be administered for several cycles to keep the reprogramming of the cell.
The pharmaceutical compositions according to the invention may further comprise at least one other active ingredient, such as an anticancer agent.
The present invention relates also to a pharmaceutical composition comprising:
(i) at least one compound of formula (I) such as defined above, and
(ii) at least one other active ingredient, such as an anticancer agent, as a combination product for simultaneous, separate or sequential use in the treatment of cancer.
This combined active ingredient can be selected from the group consisting of:
The present invention also relates to a pharmaceutical composition such as defined above for use as a drug, notably intended for the treatment of cancer.
The present invention also relates to methods for the preparation of the compounds of formula (I) according to the invention.
The compounds according to the present invention can be obtained by chemical synthesis as by one of the methods described below.
The examples, which follow, illustrate the invention without limiting its scope in any way.
The following abbreviations have been used in the following examples.
To a solution of trilobine (1) (6 mg—10.68 μmol) in 300 μL of tetrahydrofuran (THF) was added ethyl formate (46 μL—570 μmol) and 1 drop of acetic acid. The mixture was stirred 24 h at RT in inert argon atmosphere. The reaction mixture was concentrated under reduced pressure and the residue was purified by semi-preparative X-Terra RP-18 HPLC, eluting with a linear gradient H2O/MeCN with 1% formic acid (95:5 to 50:50), to give 10 as a mixture of two rotamers in proportion 50/50 (0.4 mg, 0.678 μmol, 6%).
1H NMR (500 MHz, CDCl3) δ 8.51/8.24 (1H, s, H-1″), 8.00/7.43 (1H, dd, J=8.3 Hz, J=2.2 Hz, H-14′), 7.27/7.02 (1H, m, H-13′), 7.09 (m, H-10′), 6.97-6.87 (3H, dd, H-11′, H-13, H-14), 6.71/6.67 (1H, s, H-5), 6.62/6.55 (1H, d, J=1.9 Hz, H-10), 6.42/6.36 (1H, s, H-5′), 6.39-6.24 (1H, s, H-8), 5.76/4.95 (1H, br d, J=5.5 Hz, H-1′), 4.23 (1H, m, H-3′), 3.96/3.95 (3H, s, 12-OMe), 3.89/3.88 (3H, s, 6′-OMe), 3.74 (2H, m, H-3′), 3.69/3.61 (1H, br, H-1), 3.44 (1H, m, H-3′), 3.28-2.96 (4H, m, H-α, H-α′), 2.95/2.73 (6H, m, H-3, H-4, H-4′), 2.43/2.42 (3H, s, N2-Me).
13C NMR (125 MHz, CDCl3) δ 161.2/160.9 (C-1″), 154.6 (C-12′), 150.2 (C-11), 147.6 (C-12), 146.9 (C-6′), 139.7 (C-6 or C-7 or C-8′), 139.6 (C-6 or C-7 or C-8′), 139.2 (C-6 or C-7 or C-8′), 137.3 (C-9′), 133.2 (C-9), 132.2 (C-8a), 131.4 (C-10′), 130.9/129.2 (C-14′), 130.2 (C-7′), 130.0 (C-4a), 127.7 (C-4a′), 123.5/123.1 (C-13′), 121.7/121.9 (C-11′ and C-14), 118.0/118.9 (C-8a′), 117.8/117.4 (C-10), 116.2/116.0 (C-5), 114.7/114.5 (C-8), 113.0/112.5 (C-13), 107.3/107.0 (C-5′), 65.2/65.7 (C-1), 56.6 (C-6′-OMe), 56.4 (C-12-OMe), 55.5/49.2 (C-1′), 50.4/49.9 (C-3), 46.2/45.6 (C-α′), 41.1/36.4 (C-3′), 40.2 (C—N2-Me), 38.0/38.5 (C-α), 29.2/27.1 (C-4′), 26.4/26.2 (C-4).
HRMS-ESI (m/z) calculated for C36H35N2O6 [M+H]+: 591.2490; Found: 591.2431.
To a solution of trilobine (1) (6 mg—10.68 μmol) in 200 μL of dichloromethane (CH2Cl2) and 200 μL of propan-2-ol, was added isocyanatotrimethylsilane (2 mg—17.36 μmol). The mixture was stirred 16 h at RT in inert argon atmosphere. The reaction mixture was concentrated under reduced pressure and the residue was purified by semi-preparative X-Terra RP-18 HPLC, eluting with a linear gradient H2O/MeCN with 0.02% trimethylamine (80:20 to 0:100). The collected fractions were evaporated under reduced pressure, to give 11 (4.7 mg, 7.77 μmol, 73%).
1H NMR (500 MHz, CDCl3) δ 7.96 (1H, br d, J=7.7 Hz, H-14′), 7.19 (1H, dd, J=8.5 Hz, J=2.6 Hz, H-13′), 7.05 (1H, br dd, J=8.1 Hz, J=1.8 Hz, H-10′), 6.96 (1H, dd, J=8.1 Hz, J=2.6 Hz, H-11′), 6.90 (2H, s, H-13, H-14), 6.64 (1H, s, H-10), 6.62 (1H, s, H-5), 6.39 (1H, s, H-5′), 6.25 (1H, s, H-8), 5.57 (1H, br, H-1′), 4.60 (2H, s, 1″-NH2), 3.96 (3H, s, 12-OMe), 3.88 (3H, s, 6′-OMe), 3.71-3.57 (2H, m, H-3′), 3.32 (1H, d, J=14.2 Hz, H-α′), 3.27 (1H, br, H-1), 2.96-2.81 (6H, m, H-3, H-4′, H-α, H-α′), 2.78-2.71 (3H, m, H-3, H-4), 2.61-2.54 (1H, m, H-4), 2.34 (3H, s, N2-Me).
13C NMR (125 MHz, CDCl3) δ 157.4 (C-1″), 154.9 (C-12′), 150.1 (C-11), 147.5 (C-12), 146.7 (C-6′), 139.7 (C-6 or C-7), 139.3 (C-6 or C-7), 139.1 (C-8′), 137.6 (C-9′), 135.1 (C-9), 134.9 (C-8a), 130.9 (C-14′), 130.8 (C-4a), 130.6 (C-10′), 130.2 (C-7′), 128.5 (C-4a′), 123.1 (C-13′), 122.2 (C-14), 121.5 (C-11′), 120.1 (C-8a′), 117.7 (C-10), 115.9 (C-5), 114.5 (C-8), 112.3 (C-13), 106.6 (C-5′), 67.1 (C-1), 56.6 (C-6′-OMe), 56.4 (C-12-OMe), 52.2 (C-1′), 51.2 (C-3), 46.1 (C-α′), 41.8 (C—N2-Me), 41.1 (C-3′), 40.3 (C-α), 28.0 (C-4′), 27.4 (C-4).
HRMS-ESI (m/z) calculated for C36H33N3O6 [M+H]+: 606.2599; Found: 606.2598.
To a solution of trilobine (1) (6 mg—10.68 μmol) in 300 μL of THF was added N,N-diisopropylethylamine (2 μL—11.78 μmol) and N,N-diethyl-3-isothiocyanatopropan-1-amine (2 μL—11.07 μmol). The mixture was stirred 16 h at RT in inert argon atmosphere. The reaction mixture was concentrated under reduced pressure and the residue was purified by semi-preparative X-Terra RP-18 HPLC, eluting with a linear gradient H2O/MeCN with 1% formic acid (80:20 to 0:100), to give 12 (2 mg, 2.72 μmol, 26%).
1H NMR (500 MHz, CDCl3) δ 8.16 (1H, br, H-14′), 7.92 (1H, br, 2″-NH), 7.13 (1H, dd, J=8.3 Hz, J=2.3 Hz, H-13′), 6.96 (1H, dd, J=8.4 Hz, J=2.0 Hz, H-11′), 6.90-6.84 (3H, m, H-10′, H-13, H-14), 6.68 (1H, s, H-5), 6.66 (1H, d, J=1.9 Hz, H-10), 6.63 (1H, br, H-1′), 6.47 (1H, s, H-5′), 6.44 (1H, s, H-8), 3.93 (3H, s, 12-OMe), 3.90 (3H, s, 6′-OMe), 3.89-3.82 (6H, m, H-3′, H-3″, H-5″), 3.63 (1H, br, H-1), 3.43 (1H, d, J=12.7 Hz, H-α′), 3.17-2.85 (9H, m, H-4′, H-8″, H-9″, H-α, H-α′), 2.66-2.74 (2H, m, H-4), 2.65-2.58 (2H, m, H-3), 2.28 (3H, s, N2-Me), 2.15-2.02 (2H, br, H-4″), 1.20 (6H, br t, J=6.5 Hz, H-7″, H-10″).
13C NMR (125 MHz, CDCl3) δ 180.9 (C-1″), 154.8 (C-12′), 149.5 (C-11), 147.9 (C-12), 146.8 (C-6′), 140.7 (C-6 or C-7), 139.5 (C-6 or C-7), 139.2 (C-8′), 136.0 (C-9), 132.7 (C-9), 132.3 (C-14′), 131.7 (C-4a), 130.6 (C-8a), 130.4 (C-10′), 130.0 (C-4a′ and C-7′), 132.1 (C-11′), 122.7 (C-13′), 121.1 (C-14), 119.4 (C-8a′), 117.7 (C-10), 115.7 (C-5), 115.0 (C-8), 112.6 (C-13), 106.7 (C-5′), 64.3 (C-1), 56.6 (C-6′-OMe), 56.3 (C-12-OMe), 56.1 (C-1′), 51.4 (C-3), 45.9 (C-8″ and C-9″), 44.6 (C-α′), 44.2 (C-3′ and C-3″ and C-5″), 39.2 (C—N2-Me), 36.3 (C-α), 28.2 (C-4′), 26.4 (C-4), 23.9 (C-4″), 9.0 (C7″ and C-10″).
HRMS-ESI (m/z) calculated for C43H51N4O5S [M+H]+: 735.3575; Found: 735.3572.
To a solution of trilobine (1) (6 mg—10.68 μmol) in 400 μL of CH2Cl2 was added methanesulfonyl chlorure (2 μL—25.84 μmol) and N,N-diisopropylethylamine (4.5 μL—26.46 μmol). The mixture was stirred 12 h at RT in inert argon atmosphere. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (75:25 to 20:80), to give 13 (1.2 mg, 1.88 μmol, 18%).
1H NMR (500 MHz, CDCl3) δ 8.00 (1H, dd, J=8.5 Hz, J=2.2 Hz, H-14′), 7.27-7.25 (1H, br, H-13′), 7.12 (1H, dd, J=7.9 Hz, J=1.9 Hz, H-10′), 7.02 (1H, dd, J=8.3 Hz, J=2.6 Hz, H-11′), 6.87-6.90 (2H, m, H-13, H-14), 6.65 (1H, s, H-5), 6.60 (1H, d, J=1.7 Hz, H-10), 6.39 (1H, s, H-5′), 6.19 (1H, s, H-8), 5.29 (1H, br, H-1′), 4.04 (1H, m, H-3′), 3.99 (3H, s, 12-OMe), 3.90 (3H, s, 6′-OMe), 3.57-3.63 (1H, m, H-3′), 3.50 (1H, br d, J=14.9 Hz, H-α′), 3.27 (1H, br t, J=3.3 Hz, H-1), 3.04-2.75 (7H, m, H-3, H-4, H-4′, H-α, H-α′), 2.78 (3H, s, 1″-S-Me), 2.67-2.60 (2H, m, H-3, H-4), 2.41 (3H, s, N2-Me).
13C NMR (125 MHz, CDCl3) δ 154.8 (C-12′), 150.3 (C-11), 147.4 (C-12), 147.1 (C-6′), 139.5 (C-6 or C-7), 139.2 (C-6 or C-7), 139.1 (C-8′), 137.6 (C-9′), 137.8 (C-8a), 135.2 (C-9), 130.9 (C-10′), 130.8 (C-4a), 130.3 (C-7′), 129.8 (C-14′), 126.8 (C-4a′), 123.4 (13′), 122.0 (C-14), 121.8 (C-11′), 119.0 (C-8a′), 117.5 (C-10), 116.0 (C-5), 114.5 (C-8), 112.2 (C-13), 107.0 (C-5′), 67.6 (C-1), 56.6 (C-6′-OMe), 56.4 (C-12-OMe), 54.1 (C-1′), 50.7 (C-3), 45.8 (C-α′), 42.7 (C—N2-Me), 41.1 (C-α), 40.3 (C-3′), 39.7 (C1″-S-Me), 27.8 (C-4), 26.3 (C-4′).
HRMS-ESI (m/z) calculated for C36H37N2O7S [M+H]+: 641.2316; Found: 641.2332.
To a solution of trilobine (1) (6 mg—10.68 μmol) in 300 μL of THF was added methyl isothiocyanate (1 μL—14.62 μmol) and N,N-diisopropylethylamine (2 μL—11.76 μmol). The mixture was stirred 12 h at RT in inert argon atmosphere. The reaction mixture was concentrated under reduced pressure and the residue was purified by semi-preparative X-Terra RP-18 HPLC, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (50:50 to 0:100), to give 14 (3.8 mg, 5.98 μmol, 56%).
1H NMR (500 MHz, CDCl3) δ 8.04 (1H, br, H-14′), 7.14 (1H, dd, J=8.6 Hz, J=2.2 Hz, H-13′), 6.97-6.90 (4H, m, H-10′, H-11′, H-13, H-14), 6.74 (1H, d, J=1.8 Hz, H-10), 6.66 (1H, s, H-5), 6.46 (1H, s, H-5′), 6.39 (1H, s, H-8), 5.29 (1H, br, H-1′), 6.37 (1H, m, H-3′), 5.70 (1H, br, 2″-NH), 3.96 (3H, s, 12-OMe), 3.91 (3H, s, 6′-OMe), 3.84-3.89 (1H, m, H-3′), 3.50 (1H, br d, J=13.7 Hz, H-α′), 3.37 (1H, br d, J=5.5 Hz, H-1), 3.26 (3H, d, J=4.5 Hz, 3″-NH-Me), 3.10 (1H, m, H-4′), 3.06-2.92 (5H, m, H-3, H-4′, H-α, H-α′), 2.79-2.72 (2H, m, H-3, H-4), 2.56 (1H, m, H-4), □ 2.26 (3H, s, N2-Me).
13C NMR (125 MHz, CDCl3) δ 182.0 (C-1″), 155.6 (C-12′), 149.6 (C-11), 147.9 (C-12), 147.0 (C-6′), 139.9 (C-6 or C-7 or C-8′), 139.4 (C-6 or C-7 or C-8′), 139.1 (C-6 or C-7 or C-8′), 135.8 (C-9′), 134.7 (C-9), 134.0 (C-8a), 131.8 (C-4a), 131.6 (C-14), 130.4 (C-10′), 130.3 (C-7′), 129.2 (C-4a′), 122.8 (C-13′), 122.5 (C-14), 121.2 (C-11′), 119.1 (C-8a′), 118.3 (C-10), 115.8 (C-5), 114.5 (C-8), 112.5 (C-13), 106.6 (C-5′), 66.0 (C-1), 56.7 (C-6′-OMe), 56.4 (C-12-OMe), 56.1 (C-1′), 51.8 (C-3), 44.9 (C-α′), 44.2 (C-3′), 40.4 (C—N2-Me), 38.9 (C-α), 33.4 (C3″-NH-Me), 28.0 (C-4′), 26.7 (C-4).
HRMS-ESI (m/z) calculated for C37H38N3O5S [M+H]+: 636.2527; Found: 636.2540.
To a solution of trilobine (1) (6 mg—10.68 μmol) in 300 μL of THF was added cyanogen bromide (7 μL—15.42 μmol) and N,N-diisopropylethylamine (3 μL—17.64 μmol). The mixture was stirred 24 h at RT in inert argon atmosphere. The reaction mixture was concentrated under reduced pressure and the residue was purified by semi-preparative X-Terra RP-18 HPLC, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (50:50 to 0:100), to give 15 (0.8 mg, 1.36 μmol, 13%).
1H NMR (500 MHz, CDCl3) δ 7.73 (1H, dd, J=8.4 Hz, J=1.9 Hz, H-14′), 7.30 (1H, dd, J=8.3 Hz, J=2.6 Hz, H-13′), 7.17 (1H, dd, J=8.2 Hz, J=1.9 Hz, H-10′), 7.03 (1H, dd, J=8.3 Hz, J=2.3 Hz, H-11′), 6.90-6.86 (2H, m, H-13, H-14), 6.64 (1H, s, H-5), 6.56 (1H, d, J=1.5 Hz, H-10), 6.35 (1H, s, H-5′), 6.17 (1H, s, H-8), 4.78 (1H, t, J=3.5 Hz, H-1′), 3.98 (3H, s, 12-OMe), 3.88 (3H, s, 6′-OMe), 3.57 (2H, m, H-3′), 3.48 (1H, dd, J=15.7 Hz, J=2.7 Hz, H-α′), 3.28 (1H, br, H-1), 3.07 (1H, m, H-4′), 3.06-2.92 (2H, m, H-α, H-α′), 2.86 (1H, m, H-3), 2.78-2.70 (3H, m, H-4, H-4′, H-α), 2.67-2.61 (2H, m, H-3, H-4), 2.43 (3H, s, N2-Me).
13C NMR (125 MHz, CDCl3) δ 154.9 (C-12′), 150.2 (C-11), 147.4 (C-12), 147.2 (C-6′), 139.2 (C-6 or C-7), 139.1 (C-6 or C-7), 137.3 (C-9′), 135.9 (C-8′), 135.4 (C-9), 134.1 (C-8a), 131.3 (C-10′), 130.6 (C-4a and C-7′), 129.3 (C-14′), 126.3 (C-4a′), 123.3 (C-13′), 122.1 (C-11′ and C-14), 117.8 (C-1″ and C-8a′), 117.4 (C-10), 116.1 (C-5), 114.4 (C-8), 112.4 (C-13), 106.8 (C-5′), 67.7 (C-1), 58.9 (C-1′), 56.6 (C-6′-OMe), 56.4 (C-12-OMe), 50.0 (C-3), 45.1 (C-α′), 42.7 (C-3′), 42.9 (C—N2-Me), 41.2 (C-α), 27.8 (C-4), 27.0 (C-4′).
HRMS-ESI (m/z) calculated for C36H34N3O5 [M+H]+: 588.2500; Found: 588.2493.
To a solution of trilobine 1 (6 mg—10.68 μmol) in 300 μL of ethanol was added 2 μL acrylonitrile (133.17 μmol) and 3 μL of N-ethyl-N-isopropylpropan-2-amine (11.78 μmol). The mixture was stirred 24 h at RT in inert argon atmosphere. The reaction mixture was concentrated under reduced pressure and the residue was purified by semi-preparative X-Terra RP-18 HPLC, eluting with a linear gradient H2O/MeCN with 1% formic acid (95:5 to 50:50), to give 16 (1. mg, 1.95 μmol, 18%).
1H NMR (500 MHz, MeOD) δ 7.66 (1H, dd, J=8.5 Hz, J=2.1 Hz, H-14′), 7.18 (1H, dd, J=8.3 Hz, J=2.6 Hz, H-13′), 7.14 (1H, dd, J=8.3 Hz, J=2.4 Hz, H-10′), 7.02 (1H, d, J=8.3 Hz, H-13), 6.96 (1H, dd, J=8.3 Hz, J=2.1 Hz, H-14), 6.88 (1H, dd, J=8.3 Hz, J=2.6 Hz, H-11′), 6.67 (1H, s, H-5), 6.65 (1H, d, J=2.0 Hz, H-10), 6.50 (1H, s, H-5′), 6.32 (1H, s, H-8), 4.06 (1H, br, H-1′), 3.93 (3H, s, 12-OMe), 3.83 (3H, s, 6′-OMe), 3.61 (1H, br, H-1), 3.38 (2H, m, H-3′, H-α′), 3.11 (1H, m, H-3′), 3.05-2.72 (12H, m, H-1″, H-2″, H-3, H-4, H-4′, H-α, H-α′), 2.57 (1H, m, H-4′), 2.53 (3H, s, N2-Me).
13C NMR (125 MHz, MeOD) δ 156.3 (C-12′), 151.6 (C-11), 149.6 (C-12), 148.1 (C-6′), 142.3 (C-6 or C-7), 141.5 (C-6 or C-7), 140.9 (C-9′), 138.2 (C-8′), 134.4 (C-9), 132.7 (C-8a), 132.5 (C-10′), 131.5 (C-4a), 130.8 (C-7′), 130.4 (C-14′), 129.7 (C-4a′), 123.6 (C-13′), 123.5 (C-14), 122.4 (C-11′), 121.0 (C-8a′), 120.7 (C-3″), 118.9 (C-10), 116.9 (C-5), 115.6 (C-8), 114.5 (C-13), 108.9 (C-5′), 67.8 (C-1), 61.3 (C-1′), 57.7 (C-6′-OMe and C-12-OMe), 52.0 (C-3), 50.2 (C-1″), 44.9 (C-α′), 43.6 (C-3′), 41.3 (C—N2-Me), 39.8 (C-α), 27.0 (C-4), 23.6 (C-4′), 18.1 (C-2″).
HRMS-ESI (m/z) calculated for C38H38N3O5 [M+H]+: 616.2806; Found: 616.2799.
To a solution of trilobine 1 (6 mg, 10.68 μmol) in 2 mL of DMF was added 2-bromoacetonitrile (1 μL—14.35 μmol), potassium iodide (0.5 mg—3.01 μmol) and potassium carbonate (2 mg—14.47 μmol). The mixture was stirred 24 h at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by semi-preparative X-Terra RP-18 HPLC, eluting with a linear gradient H2O/MeCN with 1% formic acid (80:20 to 0:100), to give 17 (2.3 mg, 3.82 μmol, 36%).
1H NMR (500 MHz, MeOD) δ 7.68 (1H, dd, J=8.6 Hz, J=2.2 Hz, H-14′), 7.20 (1H, dd, J=8.3 Hz, J=2.5 Hz, H-13′), 7.13 (1H, dd, J=8.3 Hz, J=2.2 Hz, H-10′), 7.01 (1H, d, J=8.2 Hz, H-13), 6.95 (1H, dd, J=8.2 Hz, J=2.2 Hz, H-14), 6.88 (1H, dd, J=8.3 Hz, J=2.6 Hz, H-11′), 6.67 (1H, s, H-5), 6.60 (1H, d, J=2.0 Hz, H-10), 6.51 (1H, s, H-5′), 6.25 (1H, s, H-8), 4.06 (1H, br, H-1′), 3.92 (3H, s, 12-OMe), 3.86 (1H, m, H-1″), 3.84 (3H, s, 6′-OMe), 3.86 (1H, d, J=17.0 Hz, H-1″), 3.62 (1H, br, H-1), 3.35 (2H, m, H-3′, H-α′), 3.09 (1H, br m, H-3′), 3.05-2.98 (3H, m, H-3, H-α), 2.94-2.71 (5H, m, H-4, H-4′, H-α, H-α′), 2.66 (1H, m, H-4′), 2.52 (3H, s, N2-Me).
13C NMR (125 MHz, MeOD) δ 156.3 (C-12′), 151.7 (C-Hz), 149.4 (C-12), 148.2 (C-6′), 142.3 (C-7), 141.3 (C-6), 140.5 (C-8′), 140.3 (C-9′), 134.4 (C-9), 133.2 (C-8a), 132.5 (C-10′), 131.9 (C-4a), 131.0 (C-7′), 130.3 (C-14′), 129.4 (C-4a′), 123.7 (C-11′), 123.6 (C-14), 122.7 (C-13′), 120.0 (C-8a′), 118.8 (C-2″ and C-10), 117.0 (C-5), 115.6 (C-8), 114.5 (C-13), 108.7 (C-5′), 67.7 (C-1), 60.3 (C-1′), 56.9 (C-6′-OMe and C-12-OMe), 51.7 (C-3), 45.1 (C-3′), 44.8 (C-α′), 42.6 (C-1″), 41.3 (C—N2-Me), 39.6 (C-α), 27.0 (C-4), 24.5 (C-4′).
HRMS-ESI (m/z) calculated for C37H36N3O5 [M+H]+: 602.2649; Found: 602.2647.
To a solution of trilobine 1 (6 mg—10.68 μmol) in 2 mL of DMF was added 9-(4-chlorobutyl)-9H-purin-6-amine (3 mg—13.29 μmol), potassium iodide (0.5 mg—3.01 μmol) and potassium carbonate (5 mg—36.18 μmol). The mixture was stirred 24 hours at 70° C. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (80.20 to 0:100), to give 18 as a mixture of two rotamers in proportion 60/40 (1.8 mg, 2.26 μmol, 21%).
1H NMR (500 MHz, MeOD) δ 8.19/8.02 (1H, s, H-14″), 8.15/8.00 (1H, s, H-8″), 7.79/7.58 (1H, br d, J=7.7 Hz, H-14′), 7.08 (1H, m, H-13′), 7.03 (1H, d, J=8.2 Hz, H-13), 6.97 (2H, m, H-10′, H-14), 6.81 (1H, br d, J=7.3 Hz, H-11′), 6.74 (1H, s, H-5), 6.66/6.54 (1H, br, H-10), 6.63 (1H, s, H-5′), 6.44/6.21 (1H, s, H-8), (1H, br d, J=7.3 Hz, H-1′), 4.37-4.20 (4H, H-3″, H-6″), 3.91 (3H, s, 12-OMe), 3.87 (3H, s, 6′-OMe), 3.80 (1H, br, H-1), 3.68-3.56 (2H, m, H-3′), 3.33 (1H, m, H-3), 3.18 (1H, m, H-α′), 3.02-2.80 (8H, H-3, H-4, H-4′, H-α, H-α′), 2.52 (3H, s, N2-Me), 2.04 (2H, m, H-5″), 1.73 (2H, m, H-4″).
13C NMR (125 MHz, MeOD) δ 157.5 (C-1″ or C-11″), 157.3 (C-1″ or C-11″), 156.7/156.5 (C-12′), 153.7/153.8 (C-14″), 151.3/150.8 (C-11), 150.8/150.7 (C-16″), 149.8/149.7 (C-12), 148.4/148.5 (C-6′), 142.9/142.6 (C-8″), 142.4 (C-6 or C-7), 141.1 (C-6 or C-7), 139.6 (C-8′), 138.5/138.3 (C-9′), 133.2 (C-9), 132.5 (C-4a), 132.3/131.5 (C-14′), 132.0/131.8 (C-10′), 131.7 (C-4a′), 130.8 (C-7′ and C-8a), 124.1/124.0 (C-14), 123.6/123.4 (C-13′), 122.3/122.2 (C-11′), 120.3 (C-10″), 119.9 (C-8a′), 119.0/118.9 (C-10), 117.0/117.1 (C-5), 115.9/115.4 (C-8), 114.6/114.5 (C-13), 108.9/108.7 (C-5′), 66.4 (C-1), 67.0/66.2 (C-3″), 57.0 (C-6′-OMe and C-12-OMe), 53.8/53.9 (C-1′), 52.6/52.1 (C-3), 46.9/46.7 (C-α′), 44.8/44.9 (C-6″), 42.0/42.1 (C-3′), 40.0 (C—N2-Me), 37.5 (C-α), 29.1 (C-4′), 27.8 (C-5″), 27.5 (C-4″), 26.4 (C-4).
HRMS-ESI (m/z) calculated for C45H46N7O7 [M+H]+: 796.3453; Found: 796.3442.
To a solution of trilobine 1 (6 mg—10.68 μmol) in 2 mL of DMF was added 9-(3-chloropropyl)-9H-purin-6-amine (3 mg—14.17 μmol), potassium iodide (0.5 mg—3.01 μmol) and potassium carbonate (5 mg—36.18 μmol). The mixture was stirred 24 hours at 70° C. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (80.20 to 0:100), to give 19 as a mixture of two rotamers in proportion 80/20 (1.8 mg, 2.30 μmol, 22%).
1H NMR (500 MHz, MeOD) δ 8.19/8.07 (1H, s, H-7″), 8.14/7.98 (1H, s, H-13″), 7.79/7.64 (1H, br d, J=8.3 Hz, H-14′), 7.09 (1H, dd, J=8.1 Hz, J=2.3 Hz, H-13′), 7.02 (1H, d, J=8.3 Hz, H-13), 6.98 (2H, m, H-10′, H-14), 6.83 (1H, dd, J=8.2 Hz, J=2.1 Hz, H-11′), 6.72 (1H, s, H-5), 6.65 (1H, br, H-10), 6.62 (1H, s, H-5′), 6.42/6.35 (1H, s, H-8), 5.34 (1H, br d, J=7.3 Hz, H-1′), 4.44-4.36 (2H, m, H-5″), 4.32-4.17 (2H, m, H-3″), 3.91 (3H, s, 12-OMe), 3.87 (3H, s, 6′-OMe), 3.74 (1H, br, H-1), 3.66 (1H, m, H-3′), 3.45 (1H, m, H-3′), 3.17-2.77 (10H, H-3, H-4, H-4′, H-α, H-α′), 2.50/2.45 (3H, s, N2-Me), 2.35 (2H, m, H-4″).
13C NMR (125 MHz, MeOD) δ 157.4 (C-1″ or C-10″), 156.9 (C-1″ or C-10″), 156.5 (C-12′), 153.9 (C-13″), 151.4 (C-11), 151.1 (C-15″), 149.7 (C-12), 148.4 (C-6′), 143.0 (C-7″), 142.5 (C-6 or C-7), 141.1 (C-6 or C-7), 139.6 (C-8′), 138.5 (C-9′), 133.5 (C-9), 132.5 (C-4a), 132.3 (C-14′), 131.9 (C-10′), 131.6 (C-4a′), 130.8 (C-7′ and C-8a), 124.0 (C-14), 123.6 (C-13′), 122.2 (C-11′), 120.2 (C-9″), 119.1 (C-10), 119.0 (C-8a′), 117.0 (C-5), 115.7/115.9 (C-8), 114.5/114.6 (C-13), 108.8/108.6 (C-5′), 66.7 (C-1), 64.7/64.4 (C-3″), 57.0 (C-6′-OMe and C-12-OMe), 53.7 (C-1′), 52.6 (C-3), 47.0 (C-α′), 42.9 (C-5″), 41.8 (C-3′), 40.2 (C—N2-Me), 37.5 (C-α), 30.6/30.4 (C-4″), 29.1/28.7 (C-4′), 26.5 (C-4).
HRMS-ESI (m/z) calculated for C44H44N7O7 [M+H]+: 782.3297; Found: 782.3296.
To a solution of O-methylcocsoline 3 (6 mg—10.68 μmol) in 2 mL of DMF was added 2-bromoacetonitrile (3 mg—25.01 μmol), potassium iodide (0.5 mg—3.01 μmol) and potassium carbonate (5 mg—36.18 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (95:5 to 0:100), to give 20 (0.2 mg, 0.33 μmol, 3.1%) and 21 (1.4 mg, 2.08 μmol, 19%).
1H NMR (500 MHz, MeOD) δ 7.66 (1H, dd, J=8.2 Hz, J=2.4 Hz, H-14′), 7.22 (1H, dd, J=8.0 Hz, J=2.2 Hz, H-10′), 7.17 (1H, dd, J=8.5 Hz, J=2.6 Hz, H-13′), 7.02-7.05 (2H, m, H-11′, H-13), 6.96 (1H, dd, J=8.3 Hz, J=2.2 Hz, H-14), 6.62 (1H, s, H-5), 6.58 (1H, d, J=2.1 Hz, H-10), 6.53 (1H, s, H-5′), 6.16 (1H, s, H-8), 4.13 (1H, br t, J=3.3 Hz, H-1′), 4.00 (1H, d, J=17.4 Hz, H-1″), 3.97 (3H, s, 12-OMe), 3.87 (3H, s, 6′-OMe), 3.78 (1H, d, J=17.4 Hz, H-1″), 3.46 (1H, br t, H-1), 3.41-3.19 (2H, m, H-3′), 3.00-2.61 (10H, m, H-3, H-4, H-4′, H-α, H-α′), 2.58 (3H, s, N2′-Me).
13C NMR (125 MHz, MeOD) δ 155.7 (C-12′), 152.0 (C-11), 149.0 (C-12), 148.1 (C-6′), 141.4 (C-6 or C-7 or C-8′ or C-9′), 141.2 (C-6 or C-7 or C-8′ or C-9′), 141.0 (C-6 or C-7 or C-8′ or C-9′), 140.5 (C-6 or C-7 or C-8′ or C-9′), 135.6 (C-8a), 135.4 (C-9), 132.6 (C-10′), 131.6 (C-4a), 131.1 (C-7′), 129.9 (C-14′), 128.8 (C-4a′), 123.8 (C-13′), 123.2 (C-11′), 122.8 (C-14), 121.1 (C-8a′), 118.0 (C-10), 116.8 (C-5), 116.7 (C-2″), 115.3 (C-8), 114.2 (C-13), 108.5 (C-5′), 66.2 (C-1), 61.4 (C-1′), 56.9 (C-6′-OMe and C-12-OMe), 50.3 (C-3), 46.0 (C-3′), 43.5 (C-α), 43.2 (C-α′), 42.5 (C-1″), 42.0 (C—N2′-Me), 28.9 (C-4), 24.6 (C-4′).
HRMS-ESI (m/z) calculated for C37H36N3O5 [M+H]+: 602.2649; Found: 602.2647.
1H NMR (500 MHz, MeOD) δ 7.75 (1H, dd, J=8.5 Hz, J=2.2 Hz, H-14′), 7.21 (1H, dd, J=8.2 Hz, J=2.4 Hz, H-10′), 7.17 (1H, dd, J=8.4 Hz, J=2.7 Hz, H-13′), 7.00-6.98 (2H, m, H-11′, H-13), 6.92 (1H, dd, J=8.2 Hz, J=2.2 Hz, H-14), 6.57 (1H, s, H-5), 6.54 (1H, d, J=2.2 Hz, H-10), 6.47 (1H, s, H-5′), 6.12 (1H, s, H-8), 4.24 (1H, br, J=3.3 Hz, H-1′), 3.98 (1H, d, J=17.5 Hz, H-1″), 3.94 (3H, s, 12-OMe), 3.88 (1H, m, H-1′″), 3.84 (3H, s, 6′-OMe), 3.76 (2H, m, H-1″ and H-1′″), 3.44 (1H, br, H-1), 3.18-2.56 (12H, m, H-3, H-3′, H-4, H-4′, H-α, H-α′).
13C NMR (125 MHz, MeOD) δ 155.7 (C-12′), 152.0 (C-11), 149.0 (C-12), 148.2 (C-6′), 141.3 (C-6 or C-7 or C-8′ or C-9′), 141.0 (C-6 or C-7 or C-8′ or C-9′), 140.7 (C-6 or C-7 or C-8′ or C-9′), 135.8 (C-8a), 135.5 (C-9), 132.5 (C-10′), 131.5 (C-4a), 131.1 (C-7′), 129.7 (C-14′), 129.0 (C-4a′), 123.8 (C-13′), 123.2 (C-11′), 122.8 (C-14), 119.9 (C-8a′), 119.0 (C-2′″), 118.1 (C-10), 116.8 (C-5), 116.7 (C-2″), 115.3 (C-8), 114.3 (C-13), 108.4 (C-5′), 66.3 (C-1), 60.3 (C-1′), 56.9 (C-6′-OMe and C-12-OMe), 49.6 (C-3), 45.2 (C-1′″ or C-3′), 44.9 (C-1′″ or C-3′), 43.6 (C-α or C-α′), 43.5 (C-α or C-α′), 42.5 (C-1″), 28.9 (C-4), 24.0 (C-4′).
HRMS-ESI (m/z) calculated for C38H34N4NaO5 [M+Na]+: 649.2421; Found: 649.2415.
To a solution of O-methylcocsoline 3 (6 mg—10.68 μmol) in 2 mL of ethanol was added acrylonitrile (3 mg—56.60 μmol) and N-ethyl-N-isopropylpropan-2-amine (0.5 mg—3.87 μmol). The mixture was stirred 24 hours in inert argon atmosphere at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.1% formic acid (95:5 to 0:100), to give 22 (2.2 mg, 3.58 μmol, 33%).
1H NMR (500 MHz, MeOD) δ 7.65 (1H, dd, J=8.5 Hz, J=2.1 Hz, H-14′), 7.20 (2H, m, H-10′, H-13′), 6.98 (2H, m, H-11′, H-13), 6.90 (1H, dd, J=8.1 Hz, J=2.0 Hz, H-14), 6.58 (1H, s, H-5), 6.56 (1H, d, J=2.0 Hz, H-10), 6.54 (1H, s, H-5′), 6.08 (1H, s, H-8), 4.29 (1H, br, H-1′), 3.93 (3H, s, 12-OMe), 3.84 (3H, s, 6′-OMe), 3.46 (1H, br t, J=3.4 Hz, H-1), 3.42-3.34 (2H, m, H-3′, H-α′), 3.14 (1H, m, H-3′), 3.05 (1H, m, H-4′), 2.97-2.92 (2H, m, H-3, H-α), 2.87-2.81 (2H, m, H-3, H-α′), 2.75-2.70 (5H, m, H-3, H-4′, H-1″, H-α), 2.74 (3H, s, N2′-Me), 2.67-2.57 (2H, m, H-4), 2.48 (2H, m, H-2″).
13C NMR (125 MHz, MeOD) δ 156.3 (C-12′), 151.8 (C-11), 149.0 (C-12), 148.6 (C-6′), 141.3 (C-8′), 140.8 (C-6 or C-7), 140.6 (C-6 or C-7), 139.4 (C-9′), 136.6 (C-9), 136.3 (C-8a), 132.8 (C-10′), 132.2 (C-4a), 131.4 (C-7′), 129.9 (C-14′), 127.8 (C-4a′), 123.9 (C-13′), 123.1 (C-11′ or C-14), 123.0 (C-11′ or C-14), 120.6 (C-3″), 118.6 (C-8a′), 118.4 (C-10), 116.9 (C-5), 115.3 (C-8), 114.3 (C-13), 108.4 (C-5′), 66.4 (C-1), 61.9 (C-1′), 57.0 (C-6′-OMe and C-12-OMe), 49.4 (C-1″), 47.0 (C-3), 46.3 (C-3′), 42.7 (C-α), 42.2 (C-α′), 41.4 (C—N2′-Me), 27.3 (C-4), 24.0 (C-4′), 17.4 (C-2″).
HRMS-ESI (m/z) calculated for C38H38N3O5 [M+H]+: 616.2806; Found: 616.2807.
To a solution of O-methylcocsoline 3 (3 mg—5.34 μmol) in 200 μL of DMF was added 9-(3-chloropropyl)-9H-purin-6-amine (13 mg—61.42 μmol), potassium iodide (0.5 mg—3.01 μmol) and potassium carbonate (8.5 mg—36.18 μmol). The mixture was stirred 24 hours at 70° C. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (95.5 to 50:50), to give 24 (1.2 mg, 1.63 μmol, 30%).
1H NMR (500 MHz, MeOD) δ 8.19 (1H, s, H-11″), 8.05 (1H, s, H-5″), 7.66 (1H, dd, J=8.5 Hz, J=2.1 Hz, H-14′), 7.25 (1H, dd, J=8.4 Hz, J=2.3 Hz, H-10′), 7.18 (1H, dd, J=8.5 Hz, J=2.6 Hz, H-13′), 7.00 (1H, dd, J=8.2 Hz, J=2.5 Hz, H-11′), 6.80 (1H, d, J=8.3 Hz, H-13), 6.56-6.52 (3H, m, H-5, H-5′, H-14), 6.49 (1H, d, J=2.1 Hz, H-10), 6.00 (1H, s, H-8), 4.24-4.28 (3H, m, H-1′, H-3″), 3.95 (3H, s, 12-OMe), 3.88 (3H, s, 6′-OMe), 3.44 (1H, br d, J=16.4 Hz, H-α′), 3.38-3.34 (2H, m, H-1, H-3′), 3.09 (1H, m, H-3′), 2.96-2.75 (6H, m, H-3, H-4′, H-α, H-α′), 2.74 (3H, s, N2′-Me), 2.67-2.58 (4H, m, H-1″, H-4, H-α), 2.49 (1H, m, H-1″), 2.02 (2H, m, H-2″).
13C NMR (125 MHz, MeOD) δ 157.4 (C-8″), 156.0 (C-12′), 153.8 (C-11″), 151.8 (C-11), 150.9 (C-13″), 148.8 (C-12), 148.6 (C-6′), 143.0 (C-5″), 141.3 (C-6 or C-7 or C-8′), 140.8 (C-6 or C-7 or C-8′), 140.7 (C-6 or C-7 or C-8′), 139.8 (C-9′), 136.4 (C-8a and C-9), 132.8 (C-10′), 131.9 (C-4a), 131.3 (C-7′), 129.8 (C-14′), 128.0 (C-4a′), 123.9 (C-13′), 123.1 (C-11′), 122.9 (C-14), 120.2 (C-7″), 119.0 (C-8a′), 118.1 (C-10), 116.8 (C-5), 115.2 (C-8), 113.9 (C-13), 108.3 (C-5′), 66.6 (C-1), 61.8 (C-1′), 57.0 (C-6′-OMe or C-12-OMe), 56.9 (C-6′-OMe or C-12-OMe), 51.3 (C-1″), 46.8 (C-3), 46.4 (C-3′), 43.2 (C-3″), 42.2 (C-α′), 41.5 (C—N2′-Me), 41.3 (C-α), 28.2 (C-2″), 27.9 (C-4), 24.2 (C-4′).
HRMS-ESI (m/z) calculated for C43H44N7O5 [M+H]+: 738.3398; Found: 738.3383.
To a solution of O-methylcocsoline 3 (3 mg—5.34 μmol) in 200 μL of DMF was added 9-(4-chlorobutyl)-9H-purin-6-amine (14 mg—62.04 μmol), potassium iodide (0.5 mg—3.01 μmol) and potassium carbonate (8.5 mg—36.18 μmol). The mixture was stirred 24 hours at 70° C. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (95.5 to 50:50), to give 25 (2.1 mg, 2.80 μmol, 52%).
1H NMR (500 MHz, MeOD) δ 8.18 (1H, s, H-12″), 8.08 (1H, s, H-6″), 7.63 (1H, dd, J=8.2 Hz, J=2.4 Hz, H-14′), 7.19 (2H, m, H-10′, H-13′), 6.96 (1H, d, J=8.4 Hz, H-13), 6.90 (2H, m, H-11′, H-14), 6.65 (1H, s, H-5), 6.57 (2H, m, H-5′, H-10), 6.17 (1H, s, H-8), 4.28 (1H, br d, J=4.1 Hz, H-1′), 4.21 (2H, t, J=7.0 Hz, H-4″), 3.92 (3H, s, 12-OMe), 3.85 (3H, s, 6′-OMe), 3.66 (1H, br, H-1), 3.40 (2H, m, H-3′, H-α′), 3.12 (1H, m, H-3′), 3.09-2.95 (3H, m, H-3, H-α), 2.87-2.79 (4H, m, H-4′, H-α, H-α′), 2.73 (3H, s, N2′-Me), 2.69 (2H, m, H-4), 2.59 (2H, m, H-1″), 1.87 (2H, m, H-3″), 1.48 (2H, m, H-2″).
13C NMR (125 MHz, MeOD) δ 157.4 (C-9″), 156.4 (C-12′), 153.8 (C-12″), 151.6 (C-11), 150.9 (C-14″), 149.3 (C-12), 148.7 (C-6′), 142.8 (C-6″), 141.9 (C-6 or C-7 or C-8′), 140.9 (C-6 or C-7 or C-8′), 140.5 (C-6 or C-7 or C-8′), 139.0 (C-9′), 134.9 (C-9), 133.8 (C8a), 132.7 (C-10′), 132.2 (C-4a), 131.2 (C-7′), 130.3 (C-14′), 128.1 (C-4a′), 123.9 (C-13′), 123.5 (C-14), 122.8 (C-11′), 120.1 (C-8″), 118.6 (C-8a′ and C-10), 117.0 (C-5), 115.4 (C-8), 114.3 (C-13), 108.7 (C-5′), 66.0 (C-1), 61.7 (C-1′), 56.9 (C-6′-OMe and C-12-OMe), 52.6 (C-1″), 47.7 (C-3), 46.2 (C-3′), 44.6 (C-4″), 41.8 (C-α′), 41.5 (C—N2′-Me), 39.8 (C-α), 28.9 (C-3″), 26.6 (C-4), 24.5 (C-2″), 24.2 (C-4′).
HRMS-ESI (m/z) calculated for C44H46N7O5 [M+H]+: 752.3555; Found: 752.3544.
To a solution of trilobine 1 (3 mg—5.34 μmol) in 200 μL of MeCN was added 9-(4-chlorobutyl)-9H-purin-6-amine (10 mg—44.31 μmol), potassium iodide (0.5 mg—3.01 μmol) and potassium carbonate (8.5 mg—36.18 μmol). The mixture was stirred 24 hours at 70° C. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (95.5 to 50:50), to give 27 (1.1 mg, 1.46 μmol, 27%).
1H NMR (500 MHz, MeOD) δ 8.16 (1H, s, H-12″), 8.13 (1H, s, H-6″), 7.42 (1H, dd, J=8.3 Hz, J=2.1 Hz, H-14′), 7.12 (1H, dd, J=8.3 Hz, J=2.7 Hz, H-13′), 7.09 (1H, dd, J=8.3 Hz, J=2.2 Hz, H-10′), 7.04 (1H, d, J=8.4 Hz, H-13), 6.99 (1H, dd, J=8.2 Hz, J=2.1 Hz, H-14), 6.85 (1H, dd, J=8.3 Hz, J=2.7 Hz, H-11′), 6.71 (1H, s, H-5), 6.64 (1H, d, J=1.9 Hz, H-10), 6.52 (1H, s, H-5′), 6.33 (1H, s, H-8), 4.30 (2H, t, J=6.8 Hz, H-4″), 4.12 (1H, d, J=5.9 Hz, H-1′), 3.93 (3H, s, 12-OMe), 3.85 (3H, s, 6′-OMe), 3.72 (1H, br, H-1), 3.31-3.27 (2H, m, H-3′, H-α′), 3.21 (1H, m, H-3), 3.12-2.99 (3H, m, H-3, H-α, H-α′), 2.94-2.86 (2H, m, H-4, H-4′), 2.82-2.65 (5H, m, H-1″, H-3, H-4, H-4′), 2.54 (3H, s, N2-Me), 2.00 (2H, m, H-3″), 1.70 (2H, br, H-2″).
13C NMR (125 MHz, MeOD) δ 157.5 (C-9″), 156.4 (C-12′), 153.8 (C-12″), 151.5 (C-11), 150.9 (C-14″), 149.6 (C-12), 148.3 (C-6′), 142.9 (C-6″), 142.4 (C-6 or C-7 or C-8′), 141.2 (C-6 or C-7 or C-8′), 140.3 (C-6 or C-7 or C-8′), 139.6 (C-9′), 133.8 (C-9), 132.5 (C-10′), 131.9 (C-4a), 131.4 (C-8a), 130.7 (C-7′), 130.5 (C-14′), 129.3 (C-4a′), 123.9 (C-14), 123.7 (C-13′), 122.6 (C-11′), 120.1 (C-8″), 119.8 (C-8a′), 119.1 (C-10), 117.0 (C-5), 115.6 (C-8), 114.6 (C-13), 109.0 (C-5′), 67.2 (C-1), 59.8 (C-1′), 57.0 (C-6′-OMe and C-12-OMe), 53.6 (C-1″), 52.4 (C-3), 44.7 (C-4″), 44.1 (C-α′), 43.0 (C-3′), 40.6 (C—N2-Me), 38.6 (C-α), 29.1 (C-3″), 26.7 (C-4), 25.5 (C-2″), 23.9 (C-4′).
HRMS-ESI (m/z) calculated for C44H46N7O5 [M+H]+: 752.3555; Found: 752.3541.
To a solution of trilobine 1 (3 mg—5.34 μmol) in 200 μL of DMF was added 9-(3-chloropropyl)-9H-purin-6-amine (10 mg—47.25 μmol), potassium iodide (0.5 mg—3.01 μmol) and triethylamine (10 μL—74.10 μmol). The mixture was stirred 24 hours at 70° C. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (95.5 to 50:50), to give 28 (1.7 mg, 2.31 μmol, 43%).
1H NMR (500 MHz, MeOD) δ 8.11 (1H, s, H-5″), 8.09 (1H, s, H-11″), 7.38 (1H, dd, J=8.3 Hz, J=2.1 Hz, H-14′), 7.10 (1H, dd, J=8.3 Hz, J=2.7 Hz, H-13′), 7.06 (1H, dd, J=8.3, J=2.1 Hz, H-10′), 7.03 (1H, d, J=8.7 Hz, H-13), 6.99 (1H, dd, J=8.3 Hz, J=2.0 Hz, H-14), 6.81 (1H, dd, J=8.2 Hz, J=2.5 Hz, H-11′), 6.71 (1H, s, H-5), 6.64 (1H, d, J=2.1 Hz, H-10), 6.46 (1H, s, H-5′), 6.30 (1H, s, H-8), 4.37 (2H, m, H-3″), 3.94 (1H, br, H-1′), 3.92 (3H, s, 12-OMe), 3.83 (3H, s, 6′-OMe), 3.77 (1H, br, H-1), 3.28-2.95 (7H, m, H-3, H-3′, H-α, H-α′), 2.94-2.60 (7H, m, H-1″, H-3′, H-4, H-4′), 2.57 (3H, s, N2-Me), 2.22 (2H, m, H-2″).
13C NMR (125 MHz, MeOD) δ 157.4 (C-8″), 156.3 (C-12′), 153.8 (C-11″), 151.5 (C-11), 150.9 (C-13″), 149.6 (C-12), 148.0 (C-6′), 142.6 (C-5″), 142.6 (C-6 or C-7 or C-8′), 141.4 (C-6 or C-7 or C-8′), 140.0 (C-6 or C-7 or C-8′ and C-9′), 133.5 (C-9), 132.5 (C-10′), 131.7 (C-4a), 130.6 (C-8a and C-14′), 130.4 (C-7′), 129.7 (C-4a′), 123.9 (C-14), 123.6 (C-13′), 122.4 (C-11′), 120.9 (C-8a′), 120.2 (C-7″), 119.2 (C-10), 117.0 (C-5), 115.7 (C-8), 114.6 (C-13), 108.9 (C-5′), 67.0 (C-1), 59.7 (C-1′), 57.0 (C-6′-OMe or C-12-OMe), 56.9 (C-6′-OMe or C-12-OMe), 52.5 (C-3), 51.7 (C-1″), 43.8 (C-3′), 43.5 (C-3″), 42.5 (C-α′), 40.6 (C—N2-Me), 38.1 (C-α), 28.4 (C-2″), 26.5 (C-4), 24.8 (C-4′).
HRMS-ESI (m/z) calculated for C43H44N7O5 [M+H]+: 738.3398; Found: 738.3387.
To a solution of trilobine 1 (3 mg—5.34 μmol) in 250 μL of DMF was added 2,5-dimethylenepyrrolidin-1-yl-4-((2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)cyclohexanecarboxylate (6 mg—18.16 μmol) and triethylamine (10 μL—74.10 μmol). The reaction mixture was stirred 18 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (95.5 to 50:50), to give 30 (2.1 mg, 2.69 μmol, 50%).
1H NMR (500 MHz, MeOD) δ 7.87 (1H, dd, J=8.6 Hz, J=2.0 Hz, H-14′), 7.07 (1H, dd, J=8.6 Hz, J=2.5 Hz, H-13′), 7.00 (1H, d, J=8.1 Hz, H-13), 6.97 (2H, m, H-10′, H-14), 6.82 (1H, m, H-11′), 6.80 (2H, s, H-11″, H-12″), 6.71 (1H, s, H-5), 6.65 (1H, d, J=2.2 Hz, H-10), 6.64 (1H, s, H-5′), 6.38 (1H, s, H-8), 5.79 (1H, br d, J=7.2 Hz, H-1′), 3.91 (3H, s, 12-OMe), 3.87 (3H, s, 6′-OMe), 3.87-3.73 (2H, m, H-3′), 3.69 (1H, br, H-1), 3.38 (2H, m, H-8″), 3.14 (1H, d, J=13.7 Hz, H-α′), 3.10-2.85 (7H, m, H-3, H-4′, H-α, H-α′), 2.82-2.77 (2H, m, H-4), 2.67 (1H, m, H-2″), 2.48 (3H, s, N2-Me), 1.98-1.93 (2H, m, H-3″ or H-4″, or H-6″ or H-7″), 1.82-1.71 (3H, m, H-3″ or H-4″, or H-6″ or H-7″ and H-5″), 1.59-1.50 (2H, m, (2H, m, H-3″ or H-4″, or H-6″ or H-7″), 1.19-1.05 (2H, m, H-3″ or H-4″, or H-6″ or H-7″).
13C NMR (125 MHz, MeOD) δ 177.1 (C-1″), 173.0 (C-10″ and C-13″), 156.5 (C-12′), 151.4 (C-11), 149.6 (C-12), 148.4 (C-6′), 142.4 (C-6 or C-7 or C-8′), 141.1 (C-6 or C-7 or C-8′), 140.0 (C-6 or C-7 or C-8′), 138.5 (C-9′), 135.5 (C-11″ and C-12″), 133.8 (C-9), 132.4 (C-4a and C-10′), 131.8 (C-14′), 131.5 (C-4a′ and C-8a), 131.0 (C-7′), 123.9 (C-14), 123.6 (C-13′), 122.1 (C-11′), 120.0 (C-8a′), 119.0 (C-10), 116.9 (C-5), 115.8 (C-8), 114.5 (C-13), 108.5 (C-5′), 66.7 (C-1), 57.0 (C-6′-OMe or C-12-OMe), 56.9 (C-6′-OMe or C-12-OMe), 52.5 (C-3), 51.8 (C-1′), 47.1 (C-α′), 44.7 (C-8″), 43.2 (C-3′), 42.2 (C-2″), 40.4 (C—N2-Me), 38.2 (C-5″), 37.7 (C-α), 31.1 (C-4″ or C-6″), 31.0 (C-4″ or C-6″), 30.0 (C-4′ or C-3″ or C-7″), 29.9 (C-4′ or C-3″ or C-7″), 26.6 (C-4).
HRMS-ESI (m/z) calculated for C47H48N3O8 [M+H]+: 782.3436; Found: 782.3436.
To a solution of acryloyl chloride (150 μL—1.85 mmol) and de N-hydroxysuccinimide (212 mg—1.85 mmol) in 250 μL of DMF was added trilobine 1 (3 mg—5.34 μmol) and triethylamine (10 μL—74.10 μmol). The mixture was stirred 18 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (95.5 to 50:50), to give 31 (2.6 mg, 4.22 μmol, 79%).
1H NMR (500 MHz, DMSO-d6) δ 8.04 (1H, dd, J=8.6 Hz, 1.7 Hz, H-14′), 7.16 (2H, m, H-10′, H-13′), 7.02 (1H, dd, J=8.0 Hz, J=2.5 Hz, H-11′), 6.99 (1H, d, J=8.3 Hz, H-13), 6.94-6.88 (2H, m, H-2″, H-14), 6.66 (1H, s, H-5), 6.63 (1H, s, H-5′), 6.47 (1H, d, J=1.9 Hz, H-10), 6.25 (1H, dd, J=16.6 Hz, J=2.2 Hz, H-3″), 6.06 (1H, s, H-8), 5.88 (1H, br, H-1′), 5.77 (1H, dd, J=10.5 Hz, J=2.2 Hz, H-3″), 4.09 (1H, m, H-3′), 3.86 (3H, s, 12-OMe), 3.79 (3H, s, 6′-OMe), 3.66 (1H, m, H-3′), 3.32 (1H, br d, J=16.0 Hz, H-α′), 3.07 (1H, br, H-1), 2.96 (1H, dd, J=15.7 Hz, J=5.5 Hz, H-α′), 2.88-2.70 (5H, m, H-3, H-4′, H-α), 2.63-2.56 (2H, m, H-4), 2.46 (1H, m, H-3), 2.25 (3H, s, N2-Me).
13C NMR (125 MHz, DMSO-d6) δ 164.1 (C-1″), 153.7 (C12′), 149.7 (C-11), 146.7 (C-12), 146.2 (C-6′), 138.8 (C-6 or C-7 or C-8′), 138.6 (C-6 or C-7 or C-8′), 138.2 (C-6 or C-7 or C-8′), 137.9 (C-9′), 135.5 (C-8a), 134.7 (C-9), 130.7 (C-4a and C-10′), 130.3 (C-14′), 128.9 (C-7′), 128.5 (C2″ and C-4a′), 128.1 (C-3″), 122.3 (C-13′), 121.8 (C-14), 121.4 (C-11′), 119.1 (C-8a′), 116.4 (C-10), 115.4 (C-5), 113.5 (C-8), 112.6 (C-13), 107.2 (C-5′), 67.2 (C-1), 56.0 (C-6′-OMe or C-12-OMe), 55.7 (C-6′-OMe or C-12-OMe), 49.9 (C-3), 49.7 (C-1′), 45.1 (C-α′), 42.4 (C—N2-Me), 40.4 (C-α or C-3′), 40.3 (C-α or C-3′), 28.2 (C-4′), 27.3 (C-4).
HRMS-ESI (m/z) calculated for C38H37N2O6 [M+H]+: 617.2646; Found: 617.2654.
To a solution of 3-chloropropanoyl chloride (100 μL—1.04 mmol) and N-hydroxysuccinimide (12 mg—107.97 μmol) in 250 μL of DMF was added trilobine 1 (3 mg—5.34 μmol) and trimethylamine (10 μL—74.10 μmol). The mixture was stirred 48 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (95.5 to 50:50), to give 32 (0.7 mg, 1.07 μmol, 20%).
1H NMR (500 MHz, MeOD) δ 7.91 (1H, dd, J=8.2 Hz, 2.0 Hz, H-14′), 7.10 (H, dd, J=8.4 Hz, J=2.6 Hz, H-13′), 7.02 (2H, m, H-10′, H-13), 6.97 (1H, dd, J=8.1 Hz, J=2.0 Hz, H-14), 6.86 (1H, dd, J=8.1 Hz, J=2.5 Hz, H-11′), 6.70 (1H, s, H-5), 6.66 (1H, d, J=2.1 Hz, H-10), 6.65 (1H, s, H-5′), 6.38 (1H, s, H-8), 5.83 (1H, br dd, J=7.6 Hz, J=1.7 Hz, H-1′), 3.92 (3H, s, 12-OMe), 3.91-3.88 (2H, m, H-3′ or H-3″), 3.87 (3H, s, 6′-OMe), 3.72 (2H, m, H-3′ or H-3″), 3.60 (1H, br, H-1), 3.22 (1H, br d, J=14.1 Hz, H-α′), 3.11-2.71 (11H, m, H-2″, H-3, H-4, H-4′, H-α, H-α′), 2.43 (3H, s, N2-Me).
13C NMR (125 MHz, MeOD) δ 171.1 (C-1″), 156.6 (C12′), 151.4 (C-11), 149.5 (C-12), 148.5 (C-6′), 142.2 (C-6 or C-7 or C-8′), 141.0 (C-6 or C-7 or C-8′), 140.0 (C-6 or C-7 or C-8′), 138.5 (C-9′), 134.1 (C-9), 132.5 (C-4a and C-14′), 132.2 (C-8a), 131.8 (C-7′ and C-10′), 131.4 (C-4a′), 123.9 (C-14), 123.6 (C-13′), 122.2 (C-11′), 119.9 (C-8a′), 119.0 (C-10), 116.9 (C-5), 115.7 (C-8), 114.4 (C-13), 108.5 (C-5′), 66.9 (C-1), 57.0 (C-6′-OMe and C-12-OMe), 52.5 (C-3), 52.0 (C-1′), 46.9 (C-α′), 43.4 (C-3′ or C-3″), 41.0 (C-3′ or C-3″), 40.6 (C—N2-Me), 38.3 (C-α), 37.7 (C-2″), 29.5 (C-4′), 26.9 (C-4).
HRMS-ESI (m/z) calculated for C38H38ClN2O6[M+H]+: 653.2398; Found: 653.2413.
To a solution of N,N-dicyclohexylcarbodiimide (100 μL—460 μmol) and N-hydroxysuccinimide (12 mg—107.97 μmol) in 250 μL of DMF was added trilobine 1 (3 mg—5.34 μmol) and triethylamine (10 μL—74.10 μmol). The mixture was stirred 48 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (95.5 to 50:50), to give 33 (0.4 mg, 0.59 μmol, 11%).
1H NMR (500 MHz, MeOD) δ 8.01 (1H, dd, J=8.6 Hz, J=2.2 Hz, H-14′), 7.95 (2H, m, H-10′), 7.00 (1H, d, J=8.3 Hz), 6.95-6.86 (3H, m), 6.72 (1H, m), 6.64 (1H, s), 6.61 (1H, s), 6.27 (1H, s, H-8), 5.92 (1H, dd, J=6.6 Hz, J=1.8 Hz, H-1′), 3.93 (3H, s, 12-OMe), 3.86 (3H, s, 6′-OMe), 3.26 (2H, m), 3.02-2.64, 10H, m), 2.35-6-2.34 (9H, m, N2-Me, N5″-Me).
13C NMR (125 MHz, MeOD) δ 167.0, 156.6, 149.3, 148.5, 142.7, 140.9, 140.2, 132.3 (C-14′), 131.8 (C-10′), 125.6, 123.6, 122.5, 119.0, 116.8, 115.4 (C-8), 114.3, 108.4 (C-5′), 68.0, 61.4, 57.0 (C-6′-OMe and C-12-O-Me), 52.3 (C-1′), 52.2, 49.9, 45.4 (C—N5″-Me), 43.3, 41.4 (C—N2-Me), 33.3, 29.7, 27.6.
HRMS-ESI (m/z) calculated for C41H44N3O6 [M+H]+: 674.3225; Found: 674.3204.
To an ice-cooled solution of product 34 (48.5 mg—79.77 μmol) in 800 μL of methanol was added iron sulphate heptahydrate (82 mg—294.95 μmol). The reaction was stirred 48 hours at RT. Then, the reaction mixture was concentrated under reduced pressure. The residue was dissolved with EDTA (10 mL—0.1M) and adjusted at pH=10 with ammoniac. The solution was extracted by a liquid-liquid extraction with dichloromethane. The organic phase was dried with sulfate magnesium and concentrated under reduced pressure. The residue was purified by silica gel column with a linear gradient of dichloromethane-methanol 1% NH3 (100:0 to 90:10), to give nortrilobine 7 (6.4 mg, 19.08 μmol, 15%), trilobine 1 (5.6 mg, 9.96 μmol, 12%), isotrilobine 2 (3.9 mg, 6.77 μmol, 9%) and O-methylcocsoline 3 (4 mg, 7.12 μmol, 9%).
1H NMR (500 MHz, MeOD) δ 7.64 (1H, dd, J=8.3 Hz, J=1.9 Hz, H-14′), 7.15 (2H, m, H-10′, H-13′), 6.96 (1H, d, J=8.3 Hz, H-13), 6.93 (1H, dd, J=8.3 Hz, J=2.7 Hz, H-11′), 6.87 (1H, dd, J=8.1 Hz, J=2.0 Hz, H-14), 6.52 (1H, s, H-5), 6.49 (1H, d, J=2.0, H-10), 6.43 (1H, s, H-5′), 6.07 (1H, s, H-8), 4.27 (1H, t, J=3.3 Hz, H-1′), 3.92 (3H, s, 12-OMe), 3.78 (3H, s, 6′-OMe), 3.27 (1H, m, H-α′), 3.22 (1H, m, H-1), 3.15-3.10 (2H, m, H-3′), 2.92-2.56 (9H, m, H-3, H-4, H-4′, H-α, H-α′), 2.39 (3H, s, N2-Me).
13C NMR (125 MHz, MeOD) δ 155.7 (C-12′), 151.8 (C-11), 148.8 (C-12), 147.8 (C-6′), 141.2 (C-6 or C-7 or C-9′), 141.0 (C-6 or C-7 or C-9′), 140.1 (C-8′), 136.4 (C-9), 136.2 (C-8a), 132.5 (C-10′), 131.0 (C-4a), 130.8 (C-7′), 129.8 (C-4a′), 129.6 (C-14′), 123.5 (C-13′), 123.2 (C-14), 122.8 (C-11′), 122.4 (C-8a′), 118.3 (C-10), 116.7 (C-5), 115.3 (C-8), 114.1 (C-13), 108.4 (C-5′), 68.8 (C-1), 56.9 (C-6′-OMe), 56.8 (C-12-OMe), 54.7 (C-1′), 50.5 (C-3), 45.6 (C-α′), 42.9 (C—N2-Me), 41.9 (C-α′), 39.3 (C-3′), 28.7 (C-4′), 28.1 (C-4).
HRMS-ESI (m/z) calculated for C35H35N2O5 [M+H]+: 563.2540; Found: 563.2541.
1H NMR (500 MHz, MeOD) δ 7.63 (1H, dd, J=8.4 Hz, J=2.0 Hz, H-14′), 7.18 (1H, dd, J=7.8 Hz, J=2.0 Hz, H-10′), 7.16 (1H, dd, J=8.2 Hz, J=2.5 Hz, H-13′), 6.98 (1H, d, J=8.3 Hz, H-13), 6.95 (1H, dd, J=8.2 Hz, J=2.8 Hz, H-11′), 6.91 (1H, dd, J=8.3 Hz, J=2.0 Hz, H-14), 6.58 (1H, s, H-5), 6.56 (1H, d, J=1.9, H-10), 6.49 (1H, s, H-5′), 6.13 (1H, s, H-8), 4.10 (1H, m, H-1′), 3.93 (3H, s, 12-OMe), 3.83 (3H, s, 6′-OMe), 3.35 (1H, m, H-α′), 3.21 (1H, m, H-1), 3.17 (H, m, H-3′), 3.00-2.88 (4H, m, H-3, H-3′, H-4′, H-α), 2.81-2.60 (5H, H-3, H-4, H-4′, H-α, H-α′), 2.57 (3H, s, N2′-Me), 2.37 (3H, s, N2-Me).
13C NMR (125 MHz, MeOD) δ 156.0 (C-12′), 151.9 (C-11), 148.9 (C-12), 148.0 (C-6′), 141.4 (C-6 or C-7 or C-8′ or C-9′), 141.0 (C-6 or C-7 or C-8′ or C-9′), 140.8 (C-6 or C-7 or C-8′ or C-9′), 140.5 (C-6 or C-7 or C-8′ or C-9′), 136.2 (C-9), 135.8 (C-8a), 132.6 (C-10′), 131.5 (C-4a) 130.9 (C-7′), 130.0 (C-14′), 128.8 (C-4a′), 123.8 (C-13′), 123.3 (C-13), 122.9 (C-11′), 121.1 (C-8a′), 118.4 (C-1), 116.8 (C-5), 115.2 (C-8), 114.2, C-13), 108.4 (C-5′), 68.9 (C-1), 61.5 (C-1′), 56.9 (C-6′-OMe and C-12-OMe), 51.8 (C-3), 46.0 (C-3′), 42.6 (C—N2-Me), 42.4 (C-α′), 42.0 (C—N2′-Me), 41.6 (C-α), 28.1 (C-4), 24.6 (C-4′).
HRMS-ESI (m/z) calculated for C36H37N2O5 [M+H]+: 577.2697; Found: 577.2698.
1H NMR (500 MHz, CDCl3) δ 7.55 (1H, dd, J=8.3 Hz, J=2.1 Hz, H-14′), 7.25 (1H, dd, J=8.3 Hz, J=2.7 Hz, H-13′), 7.14 (1H, dd, J=8.3 Hz, J=2.1 Hz, H-10′), 6.91 (3H, m, H-11′, H-13, H-14), 6.65 (1H, s, H-5), 6.64 (1H, d, J=1.9 Hz, H-10), 6.35 (H, s, H-5′), 6.21 (1H, s, H-8), 4.00 (1H, m, H-1′), 3.99 (3H, s, 12-OMe), 3.88 (3H, s, 6′-OMe), 3.62 (1H, d, J=4.6 Hz, H-1), 3.34 (1H, d, J=14.5 Hz, H-α′), 3.20-3.11 (2H, m, H-3, H-3′), 3.03-2.90 (4H, H-3, H-3′, H-4′, H-α), 2.78-2.72 (2H, m, H-4, H-α), 2.66 (1H, m, H-α′), 2.63 (3H, s, N2-Me), 2.59-2.50 (2H, m, H-4, H-4′).
13C NMR (125 MHz, CDCl3) δ 154.9 (C-12′), 150.2 (C-6), 147.7 (C-11), 146.2 (C-6′), 139.9 (C-6 or C-7 or C-8′ or C-9′), 139.6 (C-6 or C-7 or C-8′ or C-9′), 139.5 (C-6 or C-7 or C-8′ or C-9′), 139.3 (C-6 or C-7 or C-8′ or C-9′), 136.4 (C-8a), 134.7 (C-9), 131.7 (C-10′), 131.2 (C-4a), 129.7 (C-7′), 128.9 (C-14′), 127.6 (C4a′), 122.6 (C-13′), 122.1 (C-14), 121.5 (C-11′), 121.1 (C-8a′), 117.9 (C-10), 116.5 (C-5), 113.2 (C-8), 112.7 (C-13), 106.9 (C-5′), 60.7 (C-1 and C-1′), 56.5 (C-6′-OMe and C-12-OMe), 45.1 (C-3′), 44.1 (C-α), 43.2 (C-3), 42.4 (C—N2-Me), 41.7 (C-α′), 29.0 (C-4), 24.3 (C-4′).
HRMS-ESI (m/z) calculated for C35H35N2O5 [M+H]+: 563.2540; Found: 563.2544.
1H NMR (500 MHz, MeOD) δ 7.58 (1H, dd, J=8.4 Hz, J=2.2 Hz, H-14′), 7.20 (1H, dd, J=8.3 Hz, J=2.6 Hz, H-13′), 7.07 (1H, dd, J=8.3 Hz, J=2.2 Hz, H-10′), 7.04 (1H, d, J=8.4 Hz, H-13), 7.02 (1H, dd, J=8.3 Hz, J=2.0 Hz, H-14), 6.74 (1H, dd, J=8.4 Hz, J=2.5 Hz, H-11′), 6.62 (1H, s, H-5), 6.61 (1H, d, J=1.8 Hz, H-10), 6.57 (1H, s, H-5′), 6.27 (1H, s, H-8), 4.37 (1H, dd, J=5.8 Hz, J=1.5 Hz, H-1′), 3.91 (3H, s, 12-OMe), 3.80 (1H, m, H-1), 3.79 (3H, s, 6′-OMe), 3.45-3.34 (3H, m, H3′, H-α′), 3.19 (1H, m, H-3), 3.12-2.98 (H-3, H-4′, Ha), 2.93-2.88 (3H, m, H-4′, H-α, H-α′), 2.83 (1H, m, H-4), 2.71 (1H, m, H-4).
13C NMR (125 MHz, MeOD) δ 157.3 (C-12′), 151.3 (C-11), 149.8 (C-12), 148.7 (C-6′), 142.0 (C-6 or C-7), 141.0 (C6 or C-7), 139.9 (C-8′), 138.4 (C-9′), 133.8 (C-9), 133.5 (C-8a), 132.7 (C-4a and C-10′), 130.9 (C-7′), 130.5 (C-14′), 128.6 (C-4a′), 124.2 (C-14), 123.6 (C-13′), 122.3 (C-11′), 119.6 (C-10), 119.2 (C-8a′), 117.2 (C-5), 114.8 (C-13), 114.6 (C-8), 108.9 (C-5′), 59.7 (C-1), 57.0 (C-6′-OMe or C-12-OMe), 56.9 (C-6′-OMe or C-12-OMe), 54.3 (C-1′), 44.2 (C-α′), 42.6 (C-3), 40.8 (C-α), 38.2 (C-3′), 27.6 (C-4), 26.9 (C-4′).
HRMS-ESI (m/z) calculated for C34H33N2O5 [M+H]+: 549.2384; Found: 549.2388.
To a solution of nortrilobine 7 (7.5 mg—13.69 μmol) in 500 μL of tetrahydrofuran was added 3-(diethylamino)propylisothiocyanate (5 μL—29.02 μmol) and N-ethyl-N-isopropylpropan-2-amine (5 μL—29.40 μmol). The mixture was stirred 24 hours at RT in inert argon atmosphere. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (80:20 to 0:100), to give 41 (6.2 mg, 6.95 μmol, 51%).
1H NMR (500 MHz, MeOD) δ 8.35 (1H, br, H-14′), 7.12 (1H, br, H-10′), 7.07 (1H, br, H-13′), 7.00 (1H, br, H-11′), 6.97 (1H, d, J=8.2 Hz, H-13), 6.80 (1H, br d, J=8.3 Hz, H-14), 6.75 (1H, br, H-10), 6.60 (1H, br s, H-5), 6.50 (1H, br s, H-5′), 6.03 (1H, br s, H-8), 3.94 (3H, s, 12-OMe), 3.84 (3H, s, 6′-OMe), 3.80 (2H, m, H-3″ or H-3′″), 3.71-3.60 (4H, m, H-3′, H-3″ or H-3′″), 3.47 (1H, br d, J=15.9 Hz, H-α′), 3.14-3.05 (8H, m, H-7″, H-7′″, H-9″, H-9′″), 3.03-2.87 (8H, m, H-4′, H-5″, H-5′″, H-α, H-α′), 2.79-2.68 (2H, m, H-4, H-4′), 2.58 (1H, br d, J=16.3 Hz, H-4), 2.06 (2H, br, H-4″ or H-4′″), 1.91 (2H, br, H-4″ or H-4′″), 1.24 (12H, br t, J=7.1 Hz, H-8″, H-10″).
13C NMR (125 MHz, MeOD) δ 182.1 (C-1″ or C-1′″), 182.0 (C-1″ or C-1′″), 156.7 (C12′), 151.7 (C-11), 149.5 (C-12), 148.2 (C-6′), 141.6 (C-6 or C-7), 141.1 (C-6 or C-7), 140.4 (C-8′), 139.4 (C-9′), 135.7 (C-4a), 135.2 (C-9), 132.2 (C-10′ and C-14′), 131.4 (C-8a), 130.9 (C-7′), 129.9 (C-4a′), 124.2 (C-14), 123.3 (C-13′), 122.5 (C-11′), 121.4 (C-8a′), 118.7 (C-10), 117.1 (C-5), 115.0 (C-8), 114.1 (C-13), 108.5 (C-5′), 62.7 (C-1), 59.1 (C-1′), 57.0 (C-6′-OMe and C-12-OMe), 50.6 (C-5″ or C-5′″), 50.5 (C-5″ or C-5′″), 48.2 (C-7″ or C-7′″ or C-9″ or C-9′″), 48.0 (C-7″ or C-7′″ or C-9″ or C-9′″), 46.2 (C-α′), 45.9 (C-α), 44.1 (C3″ or C3″″), 43.9 (C3″ or C3″″), 42.8 (C-3′), 28.7 (C-4), 27.9 (C-4′), 25.7 (C-4″ and C-4′″), 9.6 (C-8″ or C-8′″ or C-10″ or C-10′″), 9.5 (C-8″ or C-8′″ or C-10″ or C-10′″).
HRMS-ESI (m/z) calculated for C50H64N6O5S2[M+H]+: 893.4452; Found: 893.4450.
To a solution of O-methylcosoline 3 (6.3 mg—11.21 μmol) in 300 μL of tetrahydrofuran was added N,N-diethyl-3-isothiocyanatopropan-1-amine (3 μL—11.07 μmol) and triethylamine (3 μL—22.22 μmol). The mixture was stirred 20 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 1% formic acid (80:20 to 0:100), to give 42 (6 mg, 8.17 μmol, 73%).
1H NMR (500 MHz, MeOD) δ 7.66 (1H, br dd, J=8.4 Hz, J=1.6 Hz, H-14′), 7.21 (2H, dd, J=8.5 Hz, J=2.3 Hz, H-10′, H-13′), 7.09 (1H, br dd, J=8.3 Hz, J=2.5 Hz, H-11′), 6.94 (1H, d, J=8.1 Hz, H-13), 6.78-6.74 (2H, m, H-10, H14), 6.60 (1H, s, H-5), 6.50 (1H, s, H-5′), 5.98 (1H, s, H-8), 4.20 (1H, br t, J=3.2 Hz, H-1′), 3.94 (3H, s, 12-OMe), 3.82 (3H, s, 6′-OMe), 3.69 (2H, m, H-3″), 3.39 (1H, br d, J=15.6 Hz, H-α′), 3.28 (1H, m, H-3′), 3.20 (4H, q, J=7.3 Hz, H-7″, H-9″), 3.12-3.06 (3H, m, H-3′, H-α), 3.12-3.06 (3H, m, H-3′, H-5″), 2.99 (1H, m, H-4′), 2.92-2.87 (2H, m, H-α), 2.86-2.74 (3H, m, H-4, H-4′, H-α′), 2.67 (3H, s, N2′-Me), 2.63 (1H, m, H-4), 1.97 (2H, br, H-4″), 1.28 (6H, br t, J=7.5 Hz, H-8″, H-10″).
13C NMR (125 MHz, MeOD) δ 182.3 (C-1″), 156.7 (C12′), 151.7 (C-11), 149.5 (C-12), 148.4 (C-6′), 141.6 (C-6 or C-7 or C-8′), 141.2 (C-6 or C-7 or C-8′), 140.8 (C-6 or C-7 or C-8′), 140.2 (C-9′), 135.8 (C-8a), 135.1 (C-9), 132.6 (C-10′), 131.4 (C-4a or C-7′), 131.2 (C-4a or C-7′), 129.5 (C-14′), 128.3 (C-4a′), 124.2 (C-14), 123.8 (C-13′), 122.9 (C-11′), 119.0 (C-8a′), 118.5 (C-10), 117.0 (C-5), 115.0 (C-8), 114.0 (C-13), 108.2 (C-5′), 62.8 (C-1), 62.0 (C-1′), 57.0 (C-6′-OMe or C-12-OMe), 56.9 (C-6′-OMe or C-12-OMe), 50.3 (C-5″), 48.1 (C-7″ and C-9″), 46.5 (C-3′), 46.1 (C-α), 43.6 (C-3″), 42.5 (C-α′), 41.5 (C—N2′-Me), 28.6 (C-4), 25.5 (C-4″), 24.0 (C-4′), 9.2 (C-8″ and C-10″).
HRMS-ESI (m/z) calculated for C43H51N4O5S [M+H]+: 735.3575; Found: 735.3559.
To a solution of 1,1-carbonyldiimidazole (2 g—12.33 mmol) in a mixture of DMF-MeCN (8 mL, (2:6)) was added MeNH3Cl (0.690 g—10.30 mmol). The reaction mixture was stirred 2 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by silica gel column with a linear gradient of dichloromethane-methanol (100:0 to 96:4), to give N-methyl-carbamoylimidazole (808 mg, 6.46 mmol, 68%)
To a solution of nortrilobine 7 (5 mg—9.12 μmol) in 500 μL of dicholoromethane was added N-methyl-carbamoylimidazole (3 mg—23.98 μmol) and triethylamine (4 μL—29.63 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (80:20 to 0:100), to give 43 (3.3 mg, 4.98 μmol, 55%).
1H NMR (500 MHz, CDCl3) δ 8.13 (1H, br dd, J=8.5 Hz, J=1.6 Hz, H-14′), 7.30 (1H, dd, J=8.5 Hz, J=2.7 Hz, H-13′), 7.16 (1H, dd, J=8.2 Hz, J=2.2 Hz, H-10′), 7.06 (1H, dd, J=8.2 Hz, J=2.7 Hz, H-11′), 6.91 (1H, d, J=8.1 Hz, H-13), 6.80 (1H, dd, J=8.2 Hz, J=2.2 Hz, H-14), 6.65 (1H, d, J=2.1 Hz, H-10), 6.64 (1H, s, H-5), 6.33 (1H, s, H-5′), 6.13 (1H, s, H-8), 5.73 (1H, br d, H-1′), 4.58 (1H, q, J=4.5 Hz, 2″-NH), 4.43 (1H, dd, J=13.8 Hz, J=5.0 Hz, H-3), 4.34 (1H, br d, H-1), 4.06 (1H, q, J=4.6 Hz, 2′″-NH), 4.00 (3H, s, 12-OMe), 3.86 (3H, s, 6′-OMe), 3.77 (1H, br, H-3′), 3.49 (1H, m, H-3′), 3.65 (1H, br d, J=15.0 Hz, H-α′), 3.11 (1H, dd, J=14.9 Hz, J=3.4 Hz, H-α), 2.97-2.91 (3H, m, H-3, H-4′, H-α′), 2.90 (3H, d, J=4.7 Hz, 3′″-NHMe), 2.82 (1H, dd, J=14.9 Hz, J=3.2 Hz, H-α), 2.77-2.68 (2H, m, H-4, H-4′), 2.65 (3H, d, J=4.6 Hz, 3″-NHMe), 2.50 (1H, br d, J=15.4 Hz, H-4).
13C NMR (125 MHz, CDCl3) δ 157.7 (C-1″), 157.5 (C-1′″), 154.0 (C12′), 151.0 (C-11), 147.9 (C-12), 146.6 (C-6′), 140.1 (C-6 or C-7), 139.8 (C-6 or C-7), 139.2 (C-8′ or C-9′), 139.1 (C-8′ or C-9′), 134.5 (C-9), 133.6 (C-8a), 131.1 (C-10′), 130.8 (C-4a), 130.4 (C-14′), 130.0 (C-7′), 128.2 (C-4a′), 123.4 (C-13′), 121.5 (C-11′ and C-14), 120.8 (C-8a′), 116.6 (C-10), 116.2 (C-5), 114.0 (C-8), 112.1 (C-13), 106.7 (C-5′), 59.3 (C-1), 56.6 (C-6′-OMe), 56.4 C-12-OMe), 52.5 (C-1′), 46.4 (C-α), 45.7 (C-α′), 39.7 (C-3′), 37.6 (C-3), 28.4 (C-4), 28.0 (C-3″), 27.6 (C-3′″), 27.3 (C-4′).
HRMS-ESI (m/z) calculated for C38H38N4NaO7 [M+Na]+: 685.2633; Found: 685.2616.
To a solution of trilobine 1 (12 mg—21.35 μmol) in 500 μL of dicholoromethane was added N-methyl-carbamoylimidazole (2.7 mg—21.58 μmol) and triethylamine (4 μL—29.63 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (55:45 to 40:60), to give 44 (4.7 mg, 7.59 μmol, 36%).
1H NMR (500 MHz, MeOD) δ 7.96 (1H, br dd, J=8.5 Hz, J=1.7 Hz, H-14′), 7.06 (1H, dd, J=8.4 Hz, J=2.5 Hz, H-13′), 7.00 (1H, m, H-10′), 6.99 (1H, d, J=8.3 Hz, H-13), 6.92 (1H, dd, J=8.3 Hz, J=2.0 Hz, H-14), 6.87 (1H, dd, J=8.1 Hz, J=2.4 Hz, H-11′), 6.62 (1H, d, J=1.8 Hz, H-10), 6.58 (1H, s, H-5), 6.56 (1H, s, H-5′), 6.22 (1H, s, H-8), 5.66 (1H, br d, J=6.3 Hz, H-1′), 3.91 (3H, s, 12-OMe), 3.84 (3H, s, 6′-OMe), 3.56 (2H, m, H-3′), 3.25 (2H, m, H-1, H-α′), 2.94-2.82 (6H, m, H-3, H-4′, H-α, H-α′), 2.80 (3H, s, 3″-NHMe), 2.76 (2H, m, H-3, H-4), 2.62 (1H, m, H-4), 2.31 (3H, s, N2-Me).
13C NMR (125 MHz, MeOD) δ 160.3 (C-1″), 156.6 (C12′), 151.6 (C-11), 149.3 (C-12), 148.2 (C-6′), 141.4 (C-6 or C-7), 140.8 (C-6 or C-7), 140.0 (C-8′), 139.3 (C-9′), 136.0 (C-9), 135.1 (C-8a), 132.1 (C-4a and C-14′), 131.7 (C-10′), 131.1 (C-4a′ or C-7′), 131.0 (C-4a′ or C-7′), 123.6 (C-14), 123.4 (C-13′), 122.2 (C-11′), 121.2 (C-8a′), 119.0 (C-10), 116.8 (C-5), 115.3 (C-8), 114.3 (C-13), 108.3 (C-5′), 68.1 (C-1), 57.0 (C-6′-OMe and C-12-OMe), 53.0 (C-1′), 52.2 (C-3), 47.1 (C-α′), 41.5 (C—N2-Me and C-3′), 40.3 (C-α), 28.7 (C-4′), 28.0 (C-3″), 27.6 (C-4).
HRMS-ESI (m/z) calculated for C37H38N3O6 [M+H]+: 685.2755; Found: 620.2753.
To a solution of O-methylcocsoline 3 (12 mg—21.35 μmol) in 500 μL of dicholoromethane was added N-methyl-carbamoylimidazole (2.7 mg—21.58 μmol) and triethylamine (4 μL—29.63 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (55:45 to 40:60), to give 45 (3.8 mg, 6.14 μmol, 29%).
1H NMR (500 MHz, MeOD) δ 7.63 (1H, br dd, J=8.7 Hz, J=1.3 Hz, H-14′), 7.18 (2H, m, H-10′, H-13′), 7.01 (1H, dd, J=8.3 Hz, J=2.5 Hz, H-11′), 6.95 (1H, d, J=8.3 Hz, H-13), 6.75 (1H, dd, J=8.0 Hz, J=1.7 Hz, H-14), 6.61 (1H, d, J=1.7 Hz, H-10), 6.53 (1H, s, H-5), 6.45 (1H, s, H-5′), 6.05 (1H, s, H-8), 4.84 (1H, br, H-1), 4.0 (2H, m, H-1′, H-3), 3.93 (3H, s, 12-OMe), 3.81 (3H, s, 6′-OMe), 3.29 (1H, m, H-α′), 3.14 (1H, m, H-3′), 3.05 (1H, m, H-3), 2.94-2.88 (2H, m, H-3′, H-4′), 2.82 (1H, m, H-α), 2.76-2.66 (3H, m, H-4, H-α, H-α′), 2.64 (3H, s, 3″-NHMe), 2.55 (3H, s, N2′-Me), 2.49 (1H, m, H-4).
13C NMR (125 MHz, MeOD) δ 160.3 (C-1″), 156.6 (C12′), 152.0 (C-11), 149.5 (C-12), 148.3 (C-6′), 141.8 (C-6 or C-7 or C-8′ or C-9′), 141.6 (C-6 or C-7 or C-8′ or C-9′), 141.5 (C-6 or C-7 or C-8′ or C-9′), 141.2 (C-6 or C-7 or C-8′ or C-9′), 136.5 (C-8a or C-9), 136.4 (C-8a or C-9), 133.2 (C-10′), 131.7 (C-4a), 131.3 (C-7′), 129.7 (C-14′), 129.3 (C-4a′), 123.9 (C-13′ and C-14), 123.1 (C-11′), 121.0 (C-8a′), 118.5 (C-10), 117.4 (C-5), 115.3 (C-8), 114.6 (C-13), 108.5 (C-5′), 62.4 (C-1′), 59.1 (C-1), 57.3 (C-6′-OMe or C-12-OMe), 57.2 (C-6′-OMe or C-12-OMe), 47.7 (C-α), 46.7 (C-3′), 43.5 (C-α′), 42.2 (C—N2′-Me), 39.7 (C-3), 29.2 (C-4), 28.2 (C-3″), 24.5 (C-4′).
HRMS-ESI (m/z) calculated for C37H38N3O6 [M+H]+: 685.2755; Found: 620.2749.
To a solution of O-methylcocsoline 3 (10 mg—17.79 μmol) in 500 μL of THF was added methyl isothiocyanate (2.5 μL—36.55 μmol) and N,N-diisopropylethylamine (5 μL—29.40 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (80:20 to 0:100), to give 46 (7.2 mg, 11.34 μmol, 64%).
1H NMR (500 MHz, CDCl3) δ 7.66 (1H, dd, J=8.3 Hz, J=2.0 Hz, H-14′), 7.26 (1H, dd, J=8.3 Hz, J=2.7 Hz, H-13′), 7.21 (1H, dd, J=8.3 Hz, J=2.1 Hz, H-10′), 7.08 (1H, dd, J=8.4 Hz, J=2.7 Hz, H-11′), 6.91 (1H, d, J=8.3 Hz, H-13), 6.78 (1H, dd, J=8.0 Hz, J=2.1 Hz, H-14), 6.67 (2H, m, H-5, H-10), 6.34 (1H, s, H-5′), 6.04 (1H, s, H-8), 5.42 (1H, br d, J=11.3 Hz, H-3), 5.24 (1H, q, J=4.2 Hz, 2″-NH), 4.54 (1H, br, H-1), 4.07 (1H, br t, J=3.0 Hz, H-1′), 4.01 (3H, s, 12-OMe), 3.87 (3H, s, 6′-OMe), 3.36 (1H, br dd, J=15.9 Hz, J=2.6 Hz, H-α′), 3.19-3.12 (3H, m, H-3, H-3′, H-α), 2.94 (3H, d, J=4.3 Hz, 3″-NHMe), 2.92-2.86 (3H, m, H-3′, H-4, H-4′), 2.82 (1H, dd, J=15.2 Hz, J=3.6 Hz, H-α), 2.74 (1H, dd, J=16.0 Hz, J=3.7 Hz, H-α′), 2.62 (3H, s, N2′-Me), 2.57-2.49 (2H, m, H-4, H-4′).
13C NMR (125 MHz, CDCl3) δ 182.2 (C-1″), 153.4 (C12′), 151.4 (C-11), 148.2 (C-12), 146.3 (C-6′), 140.9 (C-9′), 140.5 (C-6 or C-7), 140.2 (C-6 or C-7), 139.3 (C-8′), 133.4 (C-9), 132.4 (C-8a), 131.6 (C-10′), 133.7 (C-4a), 129.6 (C-7′), 128.7 (C-14′), 128.13 (C-4a′), 123.0 (C-13′), 121.8 (C-11′), 121.5 (C-14), 120.7 (C-8a′), 116.3 (C-5 and C-10), 113.6 (C-8), 112.1 (C-13), 106.8 (C-5′), 61.7 (C-1), 60.5 (C-1′), 56.5 (C-6′-OMe), 56.4 (C-12-OMe), 45.6 (C-3′ or C-α), 45.5 (C-3′ or C-α), 44.1 (C-3), 42.2 (C-α′ and C—N2′-Me), 32.8 (C-3″), 27.8 (C-4), 24.0 (C-4′).
HRMS-ESI (m/z) calculated for C37H38N3O5S [M+H]+: 636.2527; Found: 636.2524.
To a solution of nortrilobine 7 (5 mg—9.12 μmol) in 500 μL of THF was added methyl isothiocyanate (2 μL—29.24 μmol) and N,N-diisopropylethylamine (3 μL—17.64 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (80:20 to 0:100), to give 47 (3.2 mg, 4.61 μmol, 51%).
1H NMR (500 MHz, CDCl3) δ 8.31 (1H, br, H-14′), 7.25 (1H, dd, J=8.3 Hz, J=2.4 Hz, H-13′), 7.14 (1H, br dd, J=8.3 Hz, J=2.3 Hz, H-10′), 7.05 (1H, dd, J=8.1 Hz, J=2.6 Hz, H-11′), 6.93 (1H, d, J=8.3 Hz, H-13), 6.81 (1H, dd, J=8.3 Hz, J=1.8 Hz, H-14), 6.77 (1H, d, J=1.9 Hz, H-10), 6.69 (1H, s, H-5), 6.57 (1H, br, H-1′), 6.37 (1H, s, H-5′), 6.17 (1H, s, H-8), 5.80 (1H, q, J=4.4 Hz, 2″-NH), 5.45 (1H, br d, J=12.0 Hz, H-3), 5.20 (1H, q, J=4.3 Hz, 2″-NH), 4.53 (1H, br, H-1), 4.26 (1H, br, H-3′), 4.01 (3H, s, 12-OMe), 3.87 (3H, s, 6′-OMe), 3.70 (1H, m, H-3′), 3.42 (1H, br d, J=14.5 Hz, H-α′), 3.28 (1H, m, H-α), 3.24 (3H, d, J=4.4 Hz, 3″-NHMe), 3.15 (1H, td, J=12.8 Hz, J=2.6 Hz, H-3), 3.05 (1H, dd, J=14.9 Hz, J=5.3 Hz, H-α′), 2.99 (1H, m, H-4′), 2.93 (3H, d, J=4.5 Hz, 3″-NHMe), 2.90 (1H, m, H-4), 2.87-2.79 (2H, m, H-4′, H-α), 2.57 (1H, br d, J=16.4 Hz, H-4).
13C NMR (125 MHz, CDCl3) δ 182.2 (C-1′″), 182.0 (C-1″), 154.3 (C12′), 151.1 (C-11), 148.4 (C-12), 146.9 (C-6′), 140.5 (C-6 or C-7), 140.0 (C-6 or C-7), 139.2 (C-8′), 137.8 (C-9′), 133.3 (C-9), 132.5 (C-8a), 131.5 (C-14′), 133.3 (C-4a), 130.9 (C-10′), 130.1 (C-7′), 128.3 (C-4a′), 123.0 (C-13′), 121.8 (C-14), 121.5 (C-11′), 120.0 (C-8a′), 116.9 (C-10), 116.3 (C-5), 113.8 (C-8), 112.2 (C-13), 106.9 (C-5′), 61.8 (C-1), 57.0 (C-1′), 56.6 (C-6′-OMe), 56.4 (C-12-OMe), 45.6 (C-α′), 44.8 (C-α), 44.1 (C-3), 44.2 (C-3), 42.5 (C-3′), 33.4 (C-3″), 32.8 (C-3′″), 28.2 (C-4), 27.2 (C-4′).
HRMS-ESI (m/z) calculated for C38H38N4NaO5S [M+Na]+: 717.2156; Found: 717.2156.
To a solution of 1,1-carbonyldimidazole (200 mg—1.23 mmol) in a mixture of DMF-MeCN (8 mL, (2:6)) was added 3-(diethylamino)propylamine (194 μL—1.23 mmol). The reaction mixture was stirred 2 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by silica gel column with a linear gradient of dichloromethane-methanol (100:0 to 85:15), to give N-(3-(diethylamino)propyl)-1H-imidazole-1-carboxamide (60 mg, 0.267 mmol, 22%).
To a solution of nortrilobine 7 (6.3 mg—11.49 μmol) in 500 μL of dicholoromethane was added N-(3-(diethylamino)propyl)-1H-imidazole-1-carboxamide (10.32 mg—46.00 μmol) and triethylamine (6 μL—44.44 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (80:20 to 0:100), to give 48 (3.1 mg, 3.60 μmol, 31%).
1H NMR (500 MHz, CDCl3) δ 8.16 (1H, br d, J=8.5 Hz, H-14′), 7.30 (1H, dd, J=8.4 Hz, J=2.7 Hz, H-13′), 7.16 (1H, br dd, J=8.2 Hz, J=1.9 Hz, H-10′), 7.05 (1H, dd, J=8.2 Hz, J=2.5 Hz, H-11′), 6.90 (1H, d, J=8.3 Hz, H-13), 6.79 (1H, dd, J=8.3 Hz, J=1.8 Hz, H-14), 6.62 (1H, d, J=2.0 Hz, H-10), 6.61 (1H, s, H-5), 6.32 (1H, s, H-5′), 6.11 (1H, s, H-8), 5.80 (1H, br, H-1′), 4.65 (1H, br, H-1), 4.26 (1H, br d, J=12.0 Hz, H-3), 4.01 (1H, m, H-3′), 3.98 (3H, s, 12-OMe), 3.85 (3H, s, 6′-OMe), 3.44 (4H, m, H-3″, H-3′″), 3.33 (1H, br d, J=15.5 Hz, H-α′), 3.19 (1H, m, H-3′), 3.06-2.92 (5H, m, H-3, H-4′, H-α, H-α′), 2.89-2.76 (8H, m, H-7″, H-7′″, H-9″, H-9′″), 2.73 (1H, m, H-4), 2.69-2.61 (5H, m, H-4′, H-5″, H-5′″), 2.51 (1H, br d, J=16.3 Hz, H-4), 1.92 (2H, m, H-4″ or H-4′″), 1.73 (2H, m, H-4″ or H-4′″), 1.16 (12H, m, H-8″, H-8′″, H-10″, H-10′″).
13C NMR (125 MHz, CDCl3) δ 157.3 (C-1″ or C-1′″), 157.2 (C-1″ or C-1′″), 154.2 (C12′), 150.8 (C-11), 147.7 (C-12), 146.4 (C-6′), 139.9 (C-6 and C-7), 139.3 (C-8′ or C-9′), 139.2 (C-8′ or C-9′), 134.6 (C8a and C-9), 131.1 (C-10′), 130.2 (C-4a and C-14′), 129.8 (C-7′), 128.5 (C-4a′), 123.2 (C-13′), 121.8 (C-14), 121.5 (C-11′), 121.2 (C-8a′), 116.8 (C-10), 116.1 (C-5), 114.2 (C-8), 112.3 (C-13), 106.7 (C-5′), 58.4 (C-1), 56.6 (C-6′-OMe), 56.3 (C-12-OMe), 52.2 (C-1′), 50.0 (C-5″ and C-5′″), 46.5 (C-α), 46.0 (C7″ and C7′″ and C-9″ and C-9′″), 45.4 (C-α′), 39.2 (C-3′ and C-3″ and C-3′″), 38.1 (C-3), 28.2 (C-4), 27.4 (C-4′), 25.5 (C-4″ or C-4′″), 24.8 (C-4″ or C-4′″), 9.8 (C-8″ or C-8′″ or C-10″ or C-10′″), 9.4 (C-8″ or C-8′″ or C-10″ or C-10′″).
HRMS-ESI (m/z) calculated for C50H65N6O7 [M+H]+: 861.4909; Found: 861.4892.
To a solution of trilobine 1 (10.5 mg—18.68 μmol) in 500 μL of dicholoromethane was added N-(3-(diethylamino)propyl)-1H-imidazole-1-carboxamide (8.4 mg—37.44 μmol) and 65 trimethylamine (5 μL—37.03 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.1% formic acid (85:15 to 70:30), to give 49 (8 mg, 11.14 μmol, 59%).
1H NMR (500 MHz, CDCl3) δ 7.96 (1H, br d, J=7.4 Hz, H-14′), 7.19 (1H, br d, J=7.4 Hz, H-13′), 6.97 (2H, br m, H-10′, H-14), 6.88 (2H, br m, H-11′, H-13), 6.67 (1H, s, H-5), 6.60 (1H, br, H-10), 6.43 (1H, s, H-5′), 6.35 (1H, s, H-8), 5.67 (1H, br d, J=5.8 Hz, H-1′), 3.94 (3H, s, 12-OMe), 3.88 (3H, s, 6′-OMe), 3.69-3.59 (3H, m, H-1, H-3′), 3.49-3.40 (2H, m, H-3″), 3.28 (2H, m, H-3, H-α′), 3.11 (1H, br d, J=14.4 Hz, H-α), 3.04-2.97 (6H, m, H-5″, H-7″, H-9″), 2.94-2.83 (4H, m, H-4′, H-α, H-α′), 2.74-2.66 (3H, m, H-3, H-4), 2.35 (3H, s, N2-Me), 2.01 (2H, m, H-4″), 1.25 (6H, t, J=7.0 Hz, H-8″, H-10″).
13C NMR (125 MHz, CDCl3) δ 157.8 (C-1″), 154.4 (C12′), 149.8 (C-11), 147.7 (C-12), 146.5 (C-6′), 140.6 (C-6 or C-7), 139.7 (C-6 or C-7), 138.7 (C-8′), 137.6 (C-9′), 132.7 (C-9), 131.3 (C-14′), 131.0 (C-8a), 130.8 (C-4a), 130.6 (C-10′), 129.9 (C-4a′), 129.7 (C-7′), 123.1 (C-13′), 122.8 (C-14), 121.3 (C-11′), 120.3 (C-8a′), 117.4 (C-10), 115.8 (C-5), 114.8 (C-8), 112.5 (C-13), 106.8 (C-5′), 64.8 (C-1), 56.6 (C-6′-OMe), 56.3 (C-12-OMe), 51.4 (C-1′), 50.9 (C-3), 49.1 (C-5″), 49.2 (C-α′), 45.6 (C-7″ and C-9″), 40.9 (C-3′), 39.5 (C—N2-Me), 38.1 (C-3″), 37.3 (C-α), 28.3 (C-4′), 26.3 (C-4), 24.6 (C-4″), 8.6 (C-8″ and C-10″).
HRMS-ESI (m/z) calculated for C43H51N4O6 [M+H]+: 719.3803; Found: 719.3794.
To a solution of O-methylcocsoline 3 (10.5 mg—18.68 μmol) in 500 μl of THF was added N-(3-(diethylamino)propyl)-1H-imidazole-1-carboxamide (2.5 μL—36.55 μmol) and triethylamine (5 μL—37.03 μmol). The mixture was stirred 24 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (85:15 to 70:30), to give 50 (12 mg, 16.71 μmol, 89%).
1H NMR (500 MHz, CDCl3) δ 7.66 (1H, br d, J=7.8 Hz, H-14′), 7.28 (1H, br, H-13′), 7.17 (1H, br d, J=7.9 Hz, H-10′), 7.11 (1H, br d, J=7.9 Hz, H-11′), 6.91 (1H, d, J=8.1 Hz, H-13), 6.78 (1H, br d, J=7.9 Hz, H-14), 6.67 (1H, br, H-10), 6.63 (1H, s, H-5), 6.33 (1H, s, H-5′), 6.12 (1H, s, H-8), 4.77 (1H, br, H-1), 4.26 (1H, br d, J=11.6 Hz, H-3), 4.09 (1H, br, H-1′), 3.98 (3H, s, 12-OMe), 3.87 (3H, s, 6′-OMe), 3.41 (1H, br d, J=15.3 Hz, H-α′), 3.29-3.18 (3H, m, H-3′, H-3″), 3.08-2.94 (8H, m, H-3, H-3′, H-4′, H-7″, H-9″, H-α), 2.88 (2H, m, H-5″), 2.82-2.73 (3H, m, H-4, H-α, H-α′), 2.63 (3H, s, N2′-Me), 2.54 (2H, m, H-4, H-4′), 1.87 (2H, m, H-4″), 1.24 (6H, t, J=7.1 Hz, H-8″, H-10″).
13C NMR (125 MHz, CDCl3) δ 157.2 (C-1″), 154.4 (C12′), 150.5 (C-11), 147.8 (C-12), 146.5 (C-6′), 140.0 (C-6 or C-7 or C-8′ or C-9′), 139.8 (C-6 or C-7 or C-8′ or C-9′), 139.7 (C-6 or C-7 or C-8′ or C-9′), 134.6 (C-8a and C-9), 131.5 (C-10′), 130.2 (C-4a), 129.8 (C-7′), 128.4 (C-14′), 127.3 (C-4a′), 122.8 (C-13′), 122.0 (C-14), 121.8 (C-11′), 119.5 (C-8a′), 116.9 (C-10), 116.2 (C-5), 114.1 (C-8), 112.5 (C-13), 106.6 (C-5′), 61.0 (C-1′), 58.0 (C-1), 56.5 (C-6′-OMe), 56.4 (C-12-OMe), 48.9 (C-5″), 46.5 (C-α), 45.6 (C-7″ and C-9″), 45.2 (C-3′), 42.1 (C-α′), 41.5 (C—N2′-Me), 38.2 (C-3 or C-3″), 38.1 (C-3 or C-3″), 28.2 (C-4), 24.7 (C-4″), 23.3 (C-4′), 8.5 (C-8″ and C-10″).
HRMS-ESI (m/z) calculated for C43H51N4O6 [M+H]+: 719.3803; Found: 719.37940.
Step 1: Treatment of Product 44 with Hydrogen Peroxide
To an ice-cooled mixture of product 44 (8.6 mg—13.89 μmol) in H2O/1,4-dioxane (1:2, 450 μL) and sodium tungstate (2.5 mg—7.58 μmol) was added drop-wise hydrogen peroxide (18 μL—332.3 μmol). The reaction was stirred 1.5 hours at RT. Then manganese dioxide was added until a black precipitate. The reaction mixture was concentrated under reduced pressure and the residue was purified by silica gel column with an isocratic dichloromethane-methanol 1% NH3 (70:30).
Step 2: Treatment with Iron Sulphate Heptahydrate:
To an ice-cooled solution of product of step 1 (6.8 mg—10.71 μmol) in 400 μl of methanol was added iron sulphate heptahydrate (13 mg—46.76 μmol). The reaction was stirred 24 hours at RT. Then, the reaction mixture was concentrated under reduced pressure. The residue was dissolved with EDTA (10 mL—0.1M) and adjusted at pH=10 with ammoniac. The solution was extracted by a liquid/liquid extraction with dichloromethane. The organic phase was dried with sulfate magnesium and concentrated under reduced pressure. The residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (60:40 to 30:70), to give 51 (1.2 mg, 1.98 μmol, 14%).
1H NMR (500 MHz, MeOD) δ 7.83 (1H, dd, J=8.6 Hz, J=2.0 Hz, H-14′), 7.05 (1H, br dd, J=8.3 Hz, J=2.3 Hz, H-13′), 7.01 (1H, br d, J=8.5 Hz, H-13), 6.97 (1H, br, H-14), 6.93 (1H, br, H-10′), 6.80 (1H, br, H-11′), 6.70 (1H, br, H-10), 6.63 (2H, br s, H-5, H-5′), 6.32 (1H, br s, H-8), 5.57 (1H, br d, J=8.6 Hz, H-1′), 3.91 (3H, s, 12-OMe), 3.86 (3H, s, 6′-OMe), 3.66 (1H, m, H-3′), 3.48 (1H, br, H-1), 3.38 (1H, m, H-3′), 3.22 (1H, br d, J=13.3 Hz, H-α′), 3.07-2.83 (6H, m, H-3, H-4 or H-4′, H-α, H-α′), 2.81 (3H, br, NH-3″Me), 2.76 (2H, m, H-4 or H4′, H-α), 2.64 (1H, m, H-4 or H-4′).
13C NMR (125 MHz, MeOD) δ 160.5 (C-1″), 157.5 (C12′), 151.1 (C-11), 149.7 (C-12), 148.3 (C-6′), 141.4 (C-6 or C-7 or C-8′), 140.4 (C-6 or C-7 or C-8′), 139.8 (C-6 or C-7 or C-8′), 138.3 (C-9′), 137.0 (C-8a), 135.4 (C-9), 134.0 (C-4a), 132.5 (C-14′), 131.6 (C-10′), 131.5 (C-4a′), 131.2 (C-7′), 124.4 (C-14), 123.1 (C-13′), 121.6 (C-11′), 120.9 (C-8a′), 119.8 (C-10), 116.8 (C-5), 114.5 (C-8), 114.0 (C-13), 108.3 (C-5′), 60.2 (C-1), 57.0 (C-6′-OMe and C-12-OMe), 52.9 (C-1′), 47.3 (C-α′), 43.6 (C-3), 42.2 (C-3′), 41.1 (C-α), 29.0 (C-4 or C-4′), 28.9 (C-4 or C-4′), 28.0 (C-3″).
HRMS-ESI (m/z) calculated for C36H36N3O6 [M+H]+: 606.2599; Found: 606.2602.
To a solution of 2′-norcocsuline 5 (6 mg—10.95 μmol) in 250 μL of dicholoromethane was added N-methylcarbamoylimidazole (5.5 mg—44 μmol) and triethylamine (2 μL—14.84 μmol). The mixture was stirred 16 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (70:30 to 35:65), to give 53 (2.1 mg, 3.47 μmol, 32%).
1H NMR (500 MHz, DMSO-d6) δ 7.99 (1H, dd, J=8.5 Hz, J=1.8 Hz, H-14′), 7.12 (2H, m, H-10′, H-13′), 6.99 (1H, dd, J=8.2 Hz, J=2.6 Hz, H-11′), 6.79 (1H, d, J=8.0 Hz, H-13), 6.74 (1H, dd, J=8.2 Hz, J=1.9 Hz, H-14), 6.65 (1H, s, H-5), 6.60 (1H, s, H-5′), 6.54 (1H, q, J=4.2 Hz, 2″-NH), 6.42 (1H, d, J=1.9 Hz, H-10), 6.07 (1H, s, H-8), 5.64 (1H, m, H-1′), 3.79 (3H, s, 6′-OMe), 3.72 (1H, m, H-3′), 3.41 (1H, m, H-3′), 3.18 (1H, br, H-α′), 3.07 (1H, br t, J=3.4 Hz, H-1), 2.85-2.65 (7H, m, H-3, H-4, H-4′, H-α, H-α′), 2.64 (3H, d, J=4.4 Hz, NH-3″Me) 2.55 (1H, m, H-4), 2.46 (1H, m, H-3), 2.24 (3H, s, N2-Me).
13C NMR (125 MHz, DMSO-d6) δ 157.2 (C-1″), 154.1 (C12′), 148.7 (C-11), 145.8 (C-6′), 144.8 (C-12), 138.8 (C-6 or C-7), 138.7 (C-6 or C-7), 138.2 (C-9′), 138.0 (C-8′), 135.5 (C-8a), 132.9 (C-9), 130.6 (C-4a), 130.5 (C-10′), 130.1 (C-14′), 129.0 (C-4a′), 128.7 (C-7′), 122.2 (C-13′ or C-14), 122.1 (C-13′ or C-14), 121.2 (C-11′), 120.2 (C-8a′), 116.9 (C-10), 116.5 (C-13), 115.4 (C-5), 113.5 (C-8), 107.2 (C-5′), 67.2 (C-1), 55.9 (C-6′-OMe), 50.7 (C-1′), 50.1 (C-3), 45.1 (C-α′), 42.3 (C—N2-Me), 40.3 (C-α), 38.9 (C-3′), 27.3 (C-3″), 27.2 (C-4), 26.7 (C-4′).
HRMS-ESI (m/z) calculated for C36H36N3O6 [M+H]+: 606.2599; Found: 606.2596.
To an ice-cooled (−50° C.) mixture of cocsuline 4 (6 mg—10.68 μmol) in 600 μL of dichloromethane (600 μL) and triethylamine (2 μL—14.84 μmol) was added trifluoromethanesulfonic anhydride (2 μL—12.2 μmol). The mixture was stirred 2 hours at −50° C. and then 16 hours at RT. The reaction mixture was concentrated under reduced pressure and the residue was purified by HPLC semi-preparative X-Terra RP-18, eluting with a linear gradient H2O/MeCN with 0.02% triethylamine (80:20 to 0:100), to give 54 (4.9 mg, 7.06 μmol, 66%).
1H NMR (500 MHz, CDCl3) δ 7.63 (1H, dd, J=8.4 Hz, J=2.1, H-14′), 7.23 (1H, dd, J=8.5 Hz, J=2.6 Hz, H-13′), 7.21 (2H, m, H-10′, H-13), 7.00 (1H, dd, J=8.4 Hz, J=2.7 Hz, H-11′), 6.93 (1H, dd, J=8.3 Hz, J=1.9 Hz, H-14), 6.73 (1H, d, J=1.9 Hz, H-10), 6.63 (1H, s, H-5), 6.33 (1H, s, H-5′), 6.03 (1H, s, H-8), 4.02 (1H, br t, J=3.2 Hz, H-1′), 3.87 (3H, s, 6′-OMe), 3.36 (1H, br dd, J=15.4 Hz, J=2.5 Hz, H-α′), 3.23 (1H, br t, J=3.1 Hz, H-1), 3.17 (1H, m, H-3′), 3.00-2.81 (5H, m, H-3, H-3′, H-4′, H-α), 2.76-2.69 (2H, m, H-4, H-α′), 2.64-2.61 (2H, m, H-3, H-4), 2.60 (3H, s, N2′-Me), 2.52 (1H, m, H-4′), 2.39 (3H, s, N2-Me).
13C NMR (125 MHz, CDCl3) δ 153.3 (C12′), 152.5 (C-11), 146.3 (C-6′), 144.3 (C-12), 141.1 (C-9′), 140.0 (C-6 or C-7 or C-8′), 139.7 (C-6 or C-7 or C-8′), 139.5 (C-6 or C-7 or C-8′), 136.9 (C-9), 134.9 (C-8a), 131.6 (C-10′), 130.2 (C-4a), 129.7 (C-7′), 128.9 (C-14′), 127.8 (C-4a′), 123.0 (C-13), 122.5 (C-13′), 122.4 (C-14), 121.5 (C-11′), 120.6 (C-8a′), 119.0 (d, J=2.6 Hz, C-2″), 118.8 (C-10), 116.1 (C-5), 113.9 (C-8), 106.8 (C-5′), 67.3 (C-1), 60.6 (C-1′), 56.5 (C-6′-OMe), 50.3 (C-3), 45.3 (C-3′), 42.8 (C—N2-Me), 42.3 (C-α′), 42.1 (C—N2′-Me), 41.9 (C-α), 27.6 (C-4), 23.7 (C-4′).
HRMS-ESI (m/z) calculated for C36H34F3N2O7S [M+H]+: 695.2033; Found: 695.2117.
II. 1 hDNMT3A Assay
hDNMT3A enzyme inhibition was adapted from the restriction-based fluorescence assay protocol as described in the literature (Ceccaldi et al. ChemBioChem 2011, 12, 1337-1345). Briefly, a 5′-labelled biotin oligonucleotide is hybridized to its complementary strand labelled with 6-carboxyfluorescein at the 3′-end into a 384 well microplate (black Optiplates; Perkin Elmer) pre-coated with avidin. The duplex contains a unique CpG site overlapping with a restriction site of a methylation sensitive restriction enzyme. The human C-terminal DNMT3A (623-908a.a.), produced as described in (Gros et al. Nucl. Acids Res. 2013, 41(19):e185), was added in each well (200 ng/well) and mixed with the chemical compounds at desired concentrations and freshly prepared SAM (20 μM final concentration) to start the reaction in a total volume of 50 μL. After 1 hour incubation at 37° C. each well were washed three times with PBS, Tween-20 0.05%, NaCl (500 mM) and three more times with PBST. Specific fluorescent signals were detected with the methylation-sensitive restriction enzyme HpyCH4IV (NEB) as described and measured on a Perkin Elmer Envision detector. The percentage of inhibition is reported. The formula used to calculate the percentage of inhibition is [(X−Y)/X]×100, where X is the signal determined in the absence of the inhibitor and Y is the signal obtained in the presence of the inhibitor. The concentration at which 50% of efficacy of inhibition is observed (EC50) was determined by analysis of a concentration range of the tested compound in triplicates. The non-linear regression fittings with sigmoidal dose-response (variable slope) were performed with GraphPad Prism 4.03 (GraphPad Software).
Compounds of the invention inhibit human DNMT3A enzyme as reported in the table 1.
II.2 hDNMT1 Assay.
His-DNMT1 (182 kDa, human) was cloned, expressed and purified as described (Halby et al. ChemBioChem 2012, 13, 157-165). The reaction was performed in a 10 μL total reaction volume in low volume NBS™ 384-well microplates (Corning), containing the tested compound (up to 1% DMSO), 1 μM of a SAM/[methyl-3H] SAM (3TBq/mmol, PerkinElmer) mix in a ratio of 3-to-1 (isotopic dilution 1*:3), 0.3 μM of biotinylated hemimethylated DNA duplex (5′-GATmCGCmCGATGmCGmCGAATmCGmCGATmCGATGmCGAT-3′ and BIOT-5′-ATCGCATCGATCGCGATTCGCGCATCGGCGATC-3′), and 90 nM of DNMT1 in methylation buffer (20 mM HEPES pH 7.2, 1 mM EDTA, 50 mM KCl, 25 μg/mL BSA). The reaction was incubated at 37° C. for 2 hours. 8 μL are then transferred into a streptavidin 96-well scintillant coated Flashplate™ (PerkinElmer) containing 190 μL of 20 μM SAH in 50 mM Tris-HCl pH 7.4. The Flashplate™ was agitated at room temperature for 1 hour and read in 200 μL of 50 mM Tris-HCl pH 7.4 on TopCount NXT (PerkinElmer). The percentage of inhibition is reported. The formula used to calculate the percentage of inhibition is [(X−Y)/X]×100, where X is the signal determined in the absence of the inhibitor and Y is the signal obtained in the presence of the inhibitor.
Compounds of the invention inhibit human DNMT1 enzyme as reported in the table 2.
Since the compounds are inhibitors of DNMTs, their ability to induce the luciferase expression under the control of a methylated CMV promoter was evaluated.
KG-1 cells were stably transfected with the firefly luciferase reporter gene (luc+ from pGL3; Promega) under the control of the cytomegalovirus (CMV) promoter (from pEGFP-N1; Clontech Laboratories Inc.) partially methylated (50%). 20,000 cells per well were seeded in a 96-well plate. After 24 h incubation in the presence of the test compound or solvant (DMSO), the induction of the promoter was measured by quantification of the luciferase signal with the Brite-lite assay system (PerkinElmer) according to the manufacturer's protocol. The luminescence was measured on an EnVision multilabel plate reader (PerkinElmer), and the data are expressed as the fold induction as compared with the DMSO control. The mean of three experiments and the standard error is reported.
Compounds of the invention induced expression of the luciferase gene under the control of the methylated CMV promoter (table 3).
Their ability to demethylate the CMV promoter in the cancer cells and to open the chromatin on such promoter was also measured.
II.4 CMV Promoter Analysis
Using the Nucleosome Occupancy and Methylome Sequencing (NOMe-Seq) technique, the methylation status of the CMV promoter and the position of the nucleosomes on such promoter were both analysed.
NOMe-seq is a modified version of the methylation-dependent single promoter assay described by Miranda et al. (Curr Protoc Mol Biol. 2010 21.17.1-16) and the methylase-based DNA assay was performed as previously described with minor modifications. After nuclei extraction, GpC DNA methyltransferase M.CviPI (New England Biolabs) reactions were done in M.CviPI reaction buffer. GpC methyltransferase treatment was followed by DNA extraction, sodium bisulfite conversion, PCR amplification of the region of interest, cloning, and sequencing of individual clones to reveal the structure of single replicas as functional units. After treatment with the compounds or with DMSO, KG-1-Luc cells were centrifuged for 5 min at 500×g. Cell pellets were washed in ice-cold PBS, resuspended in 1 mL of ice-cold nuclei buffer [10 mM Tris (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1 mM EDTA, and 0.5% Nonidet P-40, plus protease inhibitors] per 2×106 cells, and incubated on ice for 10 min. Nuclei were recovered by centrifugation at 900×g for 5 min, washed twice in nuclei wash buffer [10 mM Tris (pH 7.4), 10 mM NaCl, mM MgCl2, and 0.1 mM EDTA containing protease inhibitors], and resuspended with 200 μL in 1×M.CviPI reaction buffer supplemented with 0.3 M sucrose, 160 μM SAM (New England Biolabs). 100 μL of purified genomic DNA were treated with 100 U of M.CviPI for 15 min at 37° C. in 200 μL final volume. The other part of 100 μL of purified genomic DNA were not treated with 100 U of M.CviPI but just incubated for 15 min at 37° C. to obtain CpG methylation profile. Reactions were stopped by the addition of an equal volume of stop solution [20 mM Tris.HCl (pH 7.9), 600 mM NaCl, 1% SDS, 10 mM EDTA, and 400 μg/mL Proteinase K] and incubated at 55° C. overnight. DNA was purified by phenol/chloroform extraction and ethanol precipitation. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research). Bisulfited DNA was eluted with 12 μL of water and 8 μL were used for CMV amplification.
PCR Amplification of Bisulfite-Treated DNA
The CMV promoter DNA amplification was carried on 8.3 μL eluted bisulfited DNA in 20 μL PCR reaction containing 1×KAPA2G Buffer A, 2.0 mM MgCl2, 200 μM dNTPs, 125 nM each primer and 1.0 units KAPA2G™ Robust HotStart DNA Polymerase (KapaBiosystems) on C1000 Touch™ thermal cycler 95° C. 3 min following by 95° C. for 20 sec, 55° C. for 30 sec, 72° C. for 30 sec×40 cycles and final extension at 72° C. 1 min. PCR fragments were quality controlled by agarose gel electrophoresis. Oligonucleotides used are listed in table below.
Steps of cloning and sequencing were as those described previously except that M13 PCR was used to amplify the cloned sequence in 20 μL PCR reaction volume containing 1×KAPA2G Buffer B, 2.0 mM MgCl2, 200 μM dNTPs, 125 nM each primer and 1.0 U KAPA2G™ Robust HotStart DNA Polymerase (KapaBiosystems) on C1000 Touch™ thermal cycler 95° C. 3 min following by 95° C. for 20 sec, 55° C. for 30 sec, 72° C. for 30 sec×40 cycles and final extension at 72° C. 1 min.
Sequence Alignment and Analysis of CG and GC Methylation Levels
Genomic alignment and bisulfite sequence analysis was performed largely as previously described (Berman et al. Nat Genet 2012, 44, 40-6).
BISMA (Bisulfite Sequencing DNA Methylation Analysis) software was used for CpG methylation analysis of primary bisulfite sequencing data from subcloned individual molecules (Rohde et al. BMC Bioinformatics 2010, 11, 230). Methyl Viewer (Pardo et al. Nucleic Acids Res 2011, 39, e5) was used to obtain GC methylation results and nucleosome occupancy. The ratio of methylation is representative of the chromatin accessibility by M.CviPI and nucleosome occupancy. The combined methylation status for each clone by CpG (BISMA) or GpC (Methyl Viewer) site has been assembled. The methylation status of cytosines in CpG and GpC contexts at a given position within the sequence, given as ratio of methylation, can be calculated as follows: R=[MCT/MCNT], where MCT is the percentage of Methylated Cytosines analysed DNA sequence in the Treated sample, and MCNT is the percentage of Methylated Cytosines on the same DNA sequence in a Not Treated sample. The chromatin accessibility is represented in the table 5 as ration of GpC methylation. The DNA demethylation is reported as percentage of methylated cytosine in the treated sample compared the non treated sample (Table 5).
C5 DNA Methylation Analysis at Endogenous Promoters
Cancer cell lines (colon HCT116 or leukaemia KG1) were treated with the compounds as indicated in the Data Tables and then analysed as following.
DNA Extraction and Bisulphite Treatment
DNA was isolated from cultured cells using the DNeasy Blood and Tissue Kit according to the manufacturer's specifications (Qiagen). DNA bisulphite conversion was performed on 2 μg of DNA using the EZ DNA Methylation-Gold Kit according to the manufacturer's specifications (Zymo Research) and bisulfited DNA was eluted with 10 μL of water.
Two different techniques were used to analyse the DNA methylation profile after bisulfite conversion of genomic DNA: (A) cloning and sequencing or (B) COBRA. The primers for each promoter are specified in Table 4.
A/ Cloning and Sequencing
Sequencing of bisulfite-treated DNA allowing resolution of the methylation state of every cytosine in the target sequence, at single molecule resolution, is considered as the “gold standard” for DNA methylation analysis.
CDKN2B (P15) and P73 Promoter PCR Amplification of Bisulfite-Treated DNA
Bisulfite-specific primers with a minimum length of 18 bp were designed using Primer 3 program. The target sequence of the designed primers contained no CpGs allowing amplification of both un- and hypermethylated DNAs. All primers were tested for their ability to yield high quality sequences and primers that gave rise to an amplicon of the expected size using non-bisulfite treated DNA as a template were discarded, thus ensuring the specificity for bisulfite-converted DNAs. Primers used in this study are listed in the table above. The targeted DNA amplification was set up with 2.5 μL eluted bisulfited DNA in 50 μL PCR reaction volume containing 1×PCR buffer, 1.5 mM MgCl2, 200 μM dNTPs, 200 nM each primer and 1.0 units Platinum® Taq DNA Polymerase (Invitrogen) on C1000 Touch™ thermal cycler 94° C. 2 min following by 94° C. for 30 sec, 55° C. for 30 sec, 72° C. for 1 min×35 cycles and final extension at 72° C. 6 min. PCR fragments were quality controlled by agarose gel electrophoresis.
Cloning and Sequencing
PCR amplicons were cloned with the StrataClone Ultra Blunt PCR Cloning Kit (Agilent Technologies), according to the manufacturers' instructions and up to 24 clones were picked for sequencing. First, M13 PCR was performed to amplify cloned sequence: each colony was resuspended in 50 μL water and 1 μL of each bacterial suspension was used to a 20 μL PCR reaction volume containing 1×PCR buffer, 2.5 mM MgCl2, 2 mM dNTPs, 3.2 μM each primer that anneals to sites flanking the insertion/ligation plasmid site, and 1.25 units AmpliTaq Gold DNA polymerase (Applied Biosystems) on C1000 Touch™ thermal cycler 95° C. 10 min following by 95° C. for 45 sec, 55° C. for 45 sec, 72° C. for 1 min×35 cycles and final extension at 72° C. 10 min. PCR amplicons were cleaned up using Bio-Gel-P100 (BioRad Laboratories) to remove any excess nucleotides and primers. Final sequencing was performed using a M13 primer on ABI 3100 capillary sequencers using 1/8 dilution of ABI Prism BigDye terminator V3.1 sequencing chemistry after hotstart 96° C. for 60 sec and thermocycling 96° C. for 10 sec, 50° C. for 10 sec, 60° C. for 4 min×25 cycles. Sequencing reactions were cleaned up using Sephadex® G-50 (GE Healthcare) to remove any excess nucleotides and dyes. Electrophoregram files and methylation signals at a given CpG site were quantified using the BISMA software as described above.
B/ Combined Bisulfite Restriction Analysis (COBRA)
This technique is a variation of bisulfite sequencing and combines bisulfite conversion based polymerase chain reaction with restriction digestion. DNA methylation levels are easily and quickly measured at fewer CpG sites than bisulfite-cloning-sequencing.
CDKN2A (P16) Promoter PCR Amplification of Bisulfite-Treated DNA
DNA amplification was set up with 100 ng eluted bisulfited DNA in 50 μL PCR reaction containing 1×PCR buffer, 2.5 mM MgCl2, 0.3 mM dNTPs, 400 nM each primer and 1.25 units EpiTaq HS (Takara) on C1000 Touch™ thermal cycler by 98° C. for 10 sec, 55° C. for 30 sec, 72° C. for 30 sec×40 cycles. 20 μL of PCR amplicons were digested in 30 μL by 2 units of BsiEI at 60° C. for 90 min. DNA fragments were migrated by 2% agarose gel electrophoresis and each band was quantified by Image Lab Software v2.0 (BioRad Laboratories).
Compounds of the invention are able to demethylate the promoter of several tumor suppressor genes in several cancer cells (data reported in the table here below) and to demethyate the CMV promoter, open the chromatin on such promoter and reactivate the luciferase signal (as reported in table 5).
In addition the compounds were able to demethylate tumour suppressor genes promoters in cancer cells such as P16 in colon cancer cell line HCT116 and P15 in leukemia KG1.
II.5 Anti-Proliferative Activity
Human cancer cells were obtained from the ATCC (USA) and cultivated in medium suggested by the supplier supplemented with foetal calf serum (Lonza, France), at 37° C. and under 5% CO2. To measure the anti-proliferative properties of tested molecules, 2×104 cells are seeded at day 0 in a 96 wells plate. The compounds to be tested, stored at −20° C. as 10−2 M stock solution in 100% DMSO, are freshly diluted on day 1 in the cell medium, before adding a dose range of 3.2 nM to 10 μM to the cells. Cells are treated everyday and cell viability assessed after 3 days or 7 days of treatment. The cell viability is assessed using the ATPLite kit from Perkin (ATPlite 1 Step Luminescence Assay System, ref 3016739), following the provider instructions. The raw data are analyzed with GraphPad Prism software (v4.03) to generate IC50 values corresponding to the compound concentrations giving 50% reduction in cell viability. The values presented are the mean results of at least two independent experiments. The standard errors are indicated.
Compounds of the invention present a potential anti-cancer activity on a panel of cancer cell lines (table 6).
To further test the effect of compounds on DNA methylation and gene reexpression we analysed its effect on the invasiveness capacity of metastatic melanoma WM266 cell line in a 3D model.
II.6 Spheroid Formation and Invasion Assay
After treatment with compound 1 or 42 at concentrations at which the compound is not cytotoxic, metastatic melanoma cells WM-266-4 GFP (3000 cells/condition) were allowed to form spheroids for 2 days on agarose 1% (Sigma-Aldrich, #A95-39) coated in 96-well plates, leading to ˜300 μm diameter spheroids. Then six different spheroids for each condition were individually embedded in EMEM media (Lonza, #BE12-684F) containing 1% of Bovin Collagen I (BD, #354231) and 2% SVF. The initial spheroid size and the 24 h invasion area were measured by fluorescent microscopy, using an Axiovert 200M device (5× Plan-Neofluor objective, Carl Zeiss, Germany). Fluorescent invasion areas were quantified using Image J (NIH) software on the sum of six Z-stacks images (20 μm interval) for each spheroid. Invasion Index was obtained by normalising the invasion area at 24 h by the initial spheroid area. Six individual spheroids were quantified for each condition and three independent experiments were performed. The results are illustrated in annexed
Compounds of the invention decreased the invasion index in metastatic melanoma cells.
In conclusion, the compounds inhibit DNA methylation, in vitro and in cancer cells. By their action, the compounds are able to reprogram the cancer cells towards less aggressive state and could be used to sensitize towards immunotherapies and chemotherapies.
| Number | Date | Country | Kind |
|---|---|---|---|
| 15306556.0 | Oct 2015 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2016/073581 | 10/3/2016 | WO | 00 |
