Claims
- 1. An isolated or purified polynucleotide comprising a sequence encoding a modified "a" determinant of HBsAg, wherein the modification is insertion of Asn and Thr between positions 122 and 123 of the HbsAg sequence.
- 2. The polynucleotide of claim 1, wherein the nucleotides encoding the insertion are AACACA.
- 3. The polynucleotide of claim 1, wherein the polynucleotide encodes the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4.
- 4. The polynucleotide of claim 1, wherein the polynucleotide comprises SEQ ID NO:i or SEQ ID NO: 2.
- 5. A recombinant vector comprising the polynucleotide of claim 1.
- 6. A recombinant expression vector comprising the polynucleotide of claim 1.
- 7. A transformed cell comprising the expression vector of claim 6.
- 8. A method for producing a polypeptide comprising a modified HBV "a" determinant, comprising incubating the host cells of claim 7 under conditions which allow expression of the coding sequence and production of the polypeptide.
- 9. A polynucleotide probe consisting of at least six bases of an HBV genomic sequence encoding a modified "a" determinant, wherein said six bases are AACACA, and wherein said modification is the insertion of AACACA between bases 366 and 367 of the HbsAg coding sequence; or the complement thereof.
- 10. The polynucleotide probe of claim 9, wherein the genomic sequence encoding a modified "a" determinant is selected from the group consisting of SEQ ID NO:1 and SEQ ID NO: 2.
- 11. A kit for determining the presence of mutant HBV polynucleotides, comprising the polynucleotide probe of claim 9 and a container.
- 12. A method for detecting mutant HBV nucleic acids in a test sample, comprising:
- a. reacting a test sample suspected of containing mutant HBV nucleic acids with the probe of claim 9, under conditions and for a time which allow formation of a complex between the probe and mutant HBV nucleic acids present in the sample; and
- b. detecting the complex which contains the probe.
Parent Case Info
This is a division of U.S. patent application Ser. No. 08/059,031 filed on May 7, 1993.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5279944 |
Fabrizi et al. |
Jan 1994 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
0511855 |
Nov 1992 |
EPX |
Divisions (1)
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Number |
Date |
Country |
Parent |
59031 |
May 1993 |
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