Claims
- 1. A chimeric carbamate hydrolase gene comprising regulatory regions which are functional in plant cells, operably linked to a carbamate hydrolase gene having the activity of a carbamate hydrolase from Arthrobacter oxidans.
- 2. A chimeric carbamate hydrolase gene according to claim 1, wherein the carbamate hydrolase gene comprises the sequence of FIG. 7, identified as SEQ ID NO: 6.
- 3. A plant that contains the chimeric carbamate hydrolase gene according to claim 2, characterized in that the plant expresses carbamate hydrolase activity.
- 4. A plant according to claim 3, wherein the plant is tobacco.
- 5. The chimeric carbamate hydrolase gene according to claim 1 , wherein the Arthrobacter oxidans is P 16/4/B (DSM 4038).
- 6. The chimeric carbamate hydrolase gene according to claim 1, wherein the Arthrobacter oxidans is P 67 (DSM 4039).
- 7. The chimeric carbamate hydrolase gene according to claim 1, wherein the Arthrobacter oxidans is P 75 (DSM 4040).
- 8. The chimeric carbamate hydrolase gene according to claim 1, wherein the Arthrobacter oxidans is P 11/1/-b (DSM 4041).
- 9. The chimeric carbamate hydrolase gene according to claim 1, wherein the Arthrobacter oxidans is P 15/4/A (DSM 4045).
- 10. The chimeric carbamate hydrolase gene according to claim 1, wherein the Arthrobacter oxidans is P 21/2 (DSM 4046).
- 11. The chimeric carbamate hydrolase gene according to claim 1, wherein the carbamate hydrolase shows a pH optimum of 6.8, a molecular weight in the range of 50-60 kd and an isoelectric point of pI=6.2.
- 12. A method of transforming a plant comprising
- transforming plant cells with a chimeric carbamate hydrolase gene to produce transformants, wherein the chimeric carbamate hydrolase gene comprises regulatory regions which are functional in plant cells, operably linked to a carbamate hydrolase gene having the activity of a carbamate hydrolase gene from Arthrobacter oxidans;
- selecting for transformants expressing the chimeric carbamate hydrolase gene; and
- growing the transformants expressing the chimeric carbamate hydrolase gene to produce plants.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3817384 |
May 1988 |
DEX |
|
Parent Case Info
This application is a continuation-in-part of U.S Ser. No. 07/353,871, filed on May 18, 1989 abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4810648 |
Stalker |
Mar 1989 |
|
Non-Patent Literature Citations (2)
Entry |
Radin, et al (1978) Genet Res. (Camb.) 32:85-89. |
Knowles, et al. (1981) Bull. Environ. Contam. Toxicol. 27:529-533. |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
353871 |
May 1989 |
|