Claims
- 1. A method of manufacturing an array, comprising:providing an oriented polymeric substrate; applying a coating comprising linking agents on said polymeric substrate; affixing reactants thereto to form binding sites; and relaxing the substrate, wherein the coating has a topographical surface area and a projected surface area and said topographical surface area is greater than said projected surface area.
- 2. The method of claim 1 wherein said coating forms an undulated surface.
- 3. The method of claim 1 wherein said reactants are selected from the group consisting of nucleic acids, proteins and carboxylates.
- 4. A method of manufacturing a blank array, comprising:a. stretching an elastomeric material having at least one major surface with a surface area; and b. functionalizing the major surface, thereby creating linking agents on said substrate for ultimate affixation of a reactant.
- 5. The method of claim 4, further comprising the step of allowing said stretched elastomeric material to relax, thereby reducing the surface area of said major surface and increasing the density of linking agents on the substrate.
- 6. The method of claim 4 wherein the elastomeric substrate is selected from the group consisting of a polyisoprene, a nitrite, a polyurethane, and a silicone.
- 7. The method of claim 4 wherein the linking agents comprise an azlactone moiety.
- 8. The method of claim 4 wherein the major surface is functionalized by reacting the major surface with a carboxylic acid, an amine, an epoxide, an azide and derivatives thereof.
- 9. The method of claim 4 further comprising affixing a backing to said substrate after reduction of the surface area.
Parent Case Info
This application is a divisional of U.S. patent application Ser. No. 09/287,379, filed Apr. 7, 1999, now U.S. Pat. No. 6,376,619 which is a continuation-in-part of U.S. patent application Ser. No. 09/059,427 filed Apr. 13, 1998, now abandoned.
GOVERNMENT NOTICE
This invention was made with government support under Project Number 95-08-0006 awarded by the National Institute of Standards and Technology. The government has certain rights in the invention.
US Referenced Citations (50)
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Entry |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09/059427 |
Apr 1998 |
US |
Child |
09/287379 |
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US |