Claims
- 1. A method, comprising:
a) providing i) biotin covalently attached to a solid support, said solid support comprising silicon dioxide, and ii) streptavidin; and b) non-covalently attaching said streptavidin to said biotin, thereby attaching said streptavidin to said solid support.
- 2. The method of claim 1, further comprises the step c) binding a biotin-linked biomolecule to said streptavidin attached to said solid support.
- 3. The method of claim 2, wherein said biotin-linked biomolecule is selected from the group consisting of biotin-linked proteins and biotin-linked nucleic acids.
- 4. A method, comprising:
a) providing i) photoactivatible biotin, ii) a solid support, said solid support comprising silicon dioxide, and iii) a source of light; and b) bring said photoactivatible biotin in contact with said solid support in the presence of light from said light source, under conditions such that biotin is covalently attach to said solid support.
- 5. The method of claim 4, further comprising the step c) non-covalently attaching streptavidin to said biotin attached to said solid support to create bound streptavidin.
- 6. The method of claim 5, further comprises the step d) binding a biotin-linked biomolecule to said bound streptavidin.
- 7. The method of claim 6, wherein said biotin-linked biomolecule is selected from the group consisting of biotin-linked proteins and biotin-linked nucleic acids.
- 8. A composition, comprising biotin covalently attached to a solid support, said solid support comprising silicon dioxide and said biotin non-covalently attached to streptavidin.
Government Interests
[0001] This application for patent under 35 U.S.C. 111(a) claims priority to Provisional Application Ser. No. 60/050,662, filed Jun. 24, 1997 under 35 U.S.C. 111(b). This invention was made with Government Support under Contract Number DE-FG02-93ER61656 awarded by the Department of Energy. The government has certain rights in the invention.
Provisional Applications (1)
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Number |
Date |
Country |
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60050662 |
Jun 1997 |
US |