Claims
- 1. Dermatan sulphate consisting of repeating disaccharide units of formula ##STR2## wherein one mole of iduronic acid and one mole of N-acetyl galactosamine sulphate, is linked by a 1,3 glucoside bond, said dermatan sulphate having the following characteristics:
- single band under electrophoresis in various buffer systems
- molecular weight: 20,000-40,000
- organic sulphur: 6-8%
- uronic acids: 30-36%
- ratio of sulphate groups to carboxyl groups: 1.2-1.5
- ratio of uronic acids to galactosamine: 1:1
- iduronic acid: 80-90% of the total uronic acids
- [.alpha.].sub.D : -60.degree. to -65.degree.
- heparin activity: 10 U/mg USP.
- 2. Dermatan sulphate according to claim 1, having the following characteristics:
- M.W. 35,000
- [.alpha.].sub.D -65.degree.
- ratio of sulphate groups to carboxyl groups 1.25
- ratio of uronic acids to galactosamine 1:1
- single band under electrophoresis.
- 3. Dermatan sulphate according to claim 1, having the following characteristics:
- M.W. 40,000
- [.alpha.].sub.D -65.degree.
- ratio of sulphate groups to carboxyl groups 1.2
- ratio of uronic acids to galactosamine 1:1
- single band under electrophoresis.
- 4. Dermatan sulphate according to claim 1, having the following characteristics:
- M.W. 35,000
- [.alpha.].sub.D -60.degree.
- ratio of sulphate groups to carboxyl groups 1.5
- ratio of uronic acids to galactosamine 1:1
- single band under electrophoresis.
Priority Claims (1)
Number |
Date |
Country |
Kind |
19864 A/86 |
Mar 1986 |
ITX |
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Parent Case Info
This application is a continuation application of application Ser. No. 386,152 filed Jul. 28, 1989, now abandoned, which is a divisional application of U.S. Ser. No. 027,733 filed Mar. 19, 1987, now U.S. Pat. No. 4,870,166.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
3862003 |
Okuyama et al. |
Jan 1975 |
|
4783447 |
Del Bono et al. |
Nov 1988 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
0097625 |
Jan 1984 |
EPX |
Non-Patent Literature Citations (5)
Entry |
Buddecke, Eckhart and Rudolf Drzeniek (1962), Hoppe-Scyler's Zeitschrift f. physiol. Chemie, 327:49 (abstract). |
Dheu-Andries, Marie L. and Serge Perez (1983) Carbohydrate Research, 124: 324-332. |
Indian Journal of Biochemistry & Biophysics, vol. 12, Sep. 1975, pp. 269-272, V. N. Satakopan et al., "Extraction of rat skin proteglycans with inorganic salts solutions". |
The Methodology of Connective Tissue Research, Chapter 14, pp. 137-146; H. W. Stuhlsatz et al.: "The preparation of dermatan sulphate". |
Hook et al. (1974) Biochem. J. pp. 33-43. |
Divisions (1)
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Number |
Date |
Country |
Parent |
27733 |
Mar 1987 |
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Continuations (1)
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Number |
Date |
Country |
Parent |
386152 |
Jul 1989 |
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