Claims
- 1. A chromatographic material comprising:
a porous metal oxide particle having a diameter of about 15 to 100 microns, a surface area of about 10 to 50 m.sup.2/g, and a pore size of about 200 to 600 Å; and a multi-Lewis base moiety incorporated onto the surface of the particle, wherein the multi-Lewis base moiety is an amount sufficient enough to impart cation-exchange characteristics to the particle for separations.
- 2. The chromatographic material of claim 1, wherein the porous metal oxide comprises a material selected from the group consisting of zirconia, titania, and alumina.
- 3. The chromatographic material of claim 1, wherein the multi-Lewis base moiety is a phosphate.
- 4. The chromatographic material of claim 3, wherein the phosphate comprises about 0.5 to 15% by weight of the chromatographic material.
- 5. The chromatographic material of claim 3, wherein the phosphate is derived from phosphoric acid.
- 6. The chromatographic material of claim 3, wherein the phosphate is an inorganic phosphate.
- 7. The chromatographic material of claim 1, wherein the multi-Lewis base moiety is ethylenediamine-N,N-tetra(methylenephosphonic acid).
- 8. The chromatographic material of claim 1, wherein the chromatographic material is substantially stable in an aqueous solution of a pH of about 1 to 14.
- 9. The chromatographic material of claim 1, wherein the porous metal oxide particle is a spherule.
- 10. A chromatographic column comprising:
a first end for receiving a mobile phase containing a sample; a second end opposite the first end for discharging an eluent; a body positioned between the first end and second end; and a chromatographic material disposed within the column, the chromatographic material comprising:
a porous metal oxide particle having a diameter of about 15 to 100 microns, a surface area of about 10 to 50 m.sup.2/g, and a pore size of about 200 to 600 Å; and a multi-Lewis base moiety incorporated onto the surface of the particle, wherein the multi-Lewis base moiety is an amount sufficient enough to impart cation-exchange characteristics to the particle for separations.
- 11. The chromatographic column of claim 10, wherein the porous metal oxide comprises a material selected from the group consisting of zirconia, titania, and alumina.
- 12. The chromatographic column of claim 10, wherein the multi-Lewis base moiety is a phosphate.
- 13. The chromatographic column of claim 12, wherein the phosphate comprises about 0.5 to 15% by weight of the chromatographic material.
- 14. The chromatographic column of claim 12, wherein the phosphate is derived from phosphoric acid.
- 15. The chromatographic material of claim 12, wherein the phosphate is an inorganic phosphate.
- 16. The chromatographic column of claim 10, wherein the multi-Lewis base moiety is ethylenediamine-N,N-tetra(methylenephosphonic acid).
- 17. The chromatographic column of claim 10, wherein the chromatographic material is substantially stable in an aqueous solution of a pH of about 1 to 14.
- 18. The chromatographic column of claim 10, wherein the porous metal oxide particle is a spherule.
- 19. A method for the separation of immunoproteins, the method comprising the steps of:
providing a chromatographic material, the chromatographic material comprising:
a porous metal oxide particle having a diameter of about 15 to 100 microns, a surface area of about 10 to 50 m.sup.2/g, and a pore size of about 200 to 600 Å, and a multi-Lewis base moiety incorporated onto the surface of the particle, wherein the multi-Lewis base moiety is an amount sufficient enough to impart cation-exchange characteristics to the particle for separations; and interacting an aqueous solution comprising a mobile phase and an immunoprotein sample with the chromatographic material.
- 20. The chromatographic material of claim 19, wherein the porous metal oxide comprises a material selected from the group consisting of zirconia, titania, and alumina.
- 21. The chromatographic material of claim 19, wherein the multi-Lewis base moiety is a phosphate.
- 22. The chromatographic material of claim 21, wherein the phosphate comprises about 0.5 to 15% by weight of the chromatographic material.
- 23. The chromatographic material of claim 21, wherein the phosphate is derived from phosphoric acid.
- 24. The chromatographic material of claim 21, wherein the phosphate is an inorganic phosphate.
- 25. The chromatographic material of claim 19, wherein the multi-Lewis base moiety is ethylenediamine-N,N-tetra(methylenephosphonic acid).
- 26. The chromatographic material of claim 19, wherein the chromatographic material is substantially stable in an aqueous solution of a pH of about 1 to 14.
- 27. The chromatographic material of claim 19, wherein the porous metal oxide particle is a spherule.
- 28. The method of claim 19, wherein the immunoproteins are monoclonal antibodies.
- 29. The method of claim 28, further comprising the step of purifying the monoclonal antibodies from a cell supernatant.
Parent Case Info
[0001] This application claims priority from provisional application serial No. 60/377,451, filed May 3, 2002, and which is incorporated herein by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60377451 |
May 2002 |
US |