Claims
- 1. A method of screening for a polynucleotide encoding an activity of interest, comprising:
(a) obtaining polynucleotides from an environmental sample; (b) normalizing the polynucleotides obtained from the sample; (c) generating a library from the normalized polynucleotides; (d) contacting the library with a plurality of oligonucleotide probes comprising a fluorescent marker and at least a portion of a polynucleotide sequence encoding a polypeptide of interest having a specified activity to select library clones positive for a sequence of interest; and (e) selecting clones with a fluorescent analyzer that detects fluorescence.
- 2. The method of claim 1, further comprising:
(a) contacting the selected clones with a reporter system that identifies a polynucleotide encoding the activity of interest; and (b) identifying clones capable of modulating expression or activity of the reporter system thereby identifying a polynucleotide of interest; wherein the positive clones contain a polynucleotide sequence encoding an activity of interest which is capable of catalyzing the bioactive substrate.
- 3. A method for screening polynucleotides, comprising:
(a) contacting a library of polynucleotides wherein the polynucleotides are derived from a mixed population of organism with a probe oligonucleotide labeled with a fluorescence molecule, which fluoresce upon binding of the probe to a target polynucleotide of the library, to select library polynucleotides positive for a sequence of interest; (b) separating library members that are positive for the sequence of interest with a fluorescent analyzer that detects fluorescence; and (c) expressing the selected polynucleotides to obtain polypeptides.
- 4. The method of claim 3, further comprising:
(a) contacting the polypeptides with a reporter system; and (b) identifying polynucleotides encoding polypeptides capable of modulating expression or activity of the reporter system.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. patent application Ser. No. 09/685,432, filed Oct. 10, 2000, which is a continuation-in-part of U.S. patent application Ser. No. 09/444,112, filed Nov. 22, 1999 (pending), which is a continuation-in-part of U.S. patent application Ser. No. 09/098,206, filed Jun. 16, 1998, now U.S. Pat. No. 6,174,673, which is a continuation-in-part of U.S. patent application Ser. No. 08/876,276, filed Jun. 16, 1997 (pending), the contents of which are all incorporated by reference in their entirety herein.
Divisions (1)
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Number |
Date |
Country |
Parent |
09685432 |
Oct 2000 |
US |
Child |
10145280 |
May 2002 |
US |
Continuation in Parts (3)
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Number |
Date |
Country |
Parent |
09444112 |
Nov 1999 |
US |
Child |
09685432 |
Oct 2000 |
US |
Parent |
09098206 |
Jun 1998 |
US |
Child |
09444112 |
Nov 1999 |
US |
Parent |
08876276 |
Jun 1997 |
US |
Child |
09098206 |
Jun 1998 |
US |