Claims
- 1. A glycoprotein of human immunodeficiency virus type 2 (HIV-2), wherein
- (a) said glycoprotein is a dimeric form of the transmembrane glycoprotein of HIV-2;
- (b) said glycoprotein has an apparent molecular weight of about 80 kDa (gp80) as indicated by gel electrophoresis; and
- (c) said glycoprotein is isolated from other HIV-2 proteins and glycoproteins from HIV-2 infected cells.
- 2. A non-glycosylated dimeric form of the transmembrane protein of human immunodeficiency virus type 2 (HIV-2), wherein
- (a) the glycosylated form of said protein has an apparent molecular weight of about 80 kDa as indicated by gel electrophoresis; and
- (b) said protein is isolated from other HIV-2 proteins and glycoproteins from HIV-2 infected cells.
- 3. A labeled antigen comprising a glycoprotein of human immunodeficiency virus type 2 (HIV-2), wherein
- (a) said glycoprotein is a dimeric form of the transmembrane glycoprotein of HIV-2;
- (b) said glycoprotein has an apparent molecular weight of about 80 kDa (gp80) as indicated by gel electrophoresis;
- (c) said glycoprotein is isolated from other HIV-2 proteins and glycoproteins from HIV-2 infected cells; and
- (d) said antigen is labeled with a label selected from the group consisting of a radioisotope, an enzyme, a fluorescent label, a chemiluminescent label and a chromophore label.
- 4. A labeled antigen comprising the non-glycosylated dimeric transmembrane protein of claim 2, wherein said antigen is labeled with a label selected from the group consisting of a radioisotope, an enzyme, a fluorescent label, a chemiluminescent label and a chromophore label.
- 5. An in vitro diagnostic method for the detection of the presence or absence of an antibody capable of binding to an antigen of HIV-2, said method comprising:
- contacting a biological sample with the glycoprotein of claim 1 for a time and under conditions sufficient for said glycoprotein and said antibody to form an antigen-antibody complex; and
- detecting the formation of said complex.
- 6. The method of claim 5, wherein said detection step further comprises measuring the amount of said antigen-antibody complex.
- 7. A diagnostic kit for the detection of the presence or absence of an anti-HIV-2 antibody capable of binding to an antigen of HIV-2, said kit comprising the protein of claim 1 in an amount sufficient to perform said detection.
- 8. The diagnostic kit of claim 7, wherein said protein is detectably labeled with a label selected from the group consisting of a radioisotope, an enzyme, a fluorescent label, a chemiluminescent label and a chromophore label.
- 9. The diagnostic kit of claim 7, further comprising an anti-immunoglobulin antibody capable of binding to said anti-HIV-2 antibody, wherein said anti-immunoglobulin antibody is present in an amount sufficient to perform said detection.
- 10. The diagnostic kit of claim 9, wherein said anti-immunoglobulin antibody is detectably labeled with a label selected from the group consisting of a radioisotope, an enzyme, a fluorescent label, a chemiluminescent label and a chromophore label.
CROSS-REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of application Ser. No. 204,346, filed Jun. 9, 1988, the entire disclosure of which is relied upon and incorporated by reference herein.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4839288 |
Montagmier et al. |
Jun 1989 |
|
Non-Patent Literature Citations (1)
Entry |
Veronese et al, "Identification of Simian Immunodeficiency Virus SIV.sub.MAC env Gene Products", J. Virology, vol. 63(3):1416-1419 (1989). |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
204346 |
Jun 1988 |
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