HIV REPORTER CELL LINE FOR SCREENING ANTI-SENSE AGENTS

Information

• Research Project
• 3489465

• ApplicationId

  3489465

• Core Project Number

  R43AI031315

• Full Project Number

  1R43AI031315-01

• Serial Number

  31315

• FOA Number

• Sub Project Id

• Project Start Date

  4/1/1991 - 33 years ago

• Project End Date

  9/30/1991 - 32 years ago

• Program Officer Name

• Budget Start Date

  4/1/1991 - 33 years ago

• Budget End Date

  9/30/1991 - 32 years ago

• Fiscal Year

  1991

• Support Year

  1

• Suffix

• Award Notice Date

  3/29/1991 - 33 years ago

Organizations

• Epoch Biosciences, Inc.

A secretable alkaline phosphatase (SPAP) reporter gene will be substituted for a portion of the env gene in an HIV proviral DNA clone. Expression of the reporter gene will be regulated by a complete complement of viral cis and trans acting control elements, such that the level of extracellular alkaline phosphatase will serve as an indicator of viral gag, pol, and env regions subject to normal regulation by the viral coded tat, rev, and nef gene products. The H9 cell line will be transfected with this construct and the transient expression of SPAP, p24, and reverse transcriptase characterized. The feasibility of using the SPAP as a marker to detect antiviral activity will be assessed by monitoring the effect of three antisense oligonucleotides (ODNs) targeted to critical sites on the HIV genome in the transfected H9 cells.

IC Name

• Activity

  R43

• Administering IC

  AI

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  856

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  SSS

• Study Section Name

• Organization Name

  EPOCH BIOSCIENCES, INC.

• Organization Department

• Organization DUNS

• Organization City

  BOTHELL

• Organization State

  WA

• Organization Country

  UNITED STATES

• Organization Zip Code

  980213906

• Organization District

  UNITED STATES