Claims
- 1. A method for determining the presence in a physiological sample of a first antigen in the potential presence of a second antigen, where said first and second antigens are characterized by being members of a polymorphic group of alleles which share numerous epitopes, wherein a combination of monoclonal antibodies is used, a first antibody which is cross-reactive to both antigens, but not other common members of said group of alleles, and a second antibody which binds to only said second antigen of said first and second antigens, said method comprising:
- combining said sample with said second antibody bound to a solid support to form a liquid assay medium and depleting said second antigen by separating said liquid assay medium from said solid support;
- combining said second antigen depleted sample with three different antibodies bound separately to three different solid supports; a first support having an anti-first species antibody of a second species; a second support having said first antibody; and a third support having said second antibody;
- adding to said first, second and third supports an enzyme conjugate of a first species antibody binding to an epitope common to all members of said polymorphic group of alleles with said first and second antigens, followed by adding a substrate producing a detectable product; and
- determining the presence of enzyme as to each of said first, second and third supports by means of said detectable product,
- wherein a negative value of said fast support indicates the assay method failed;
- a positive value of said second support as compared to said third support indicates the presence of said first antigen.
- 2. A method according to claim 1, wherein said first antigen is HLA-B27 and said second antigen is HLA-B7.
- 3. A method according to claim 2, wherein said enzyme conjugate is a conjugate of horse radish peroxidase and anti-.beta..sub.2 -microglobulin.
- 4. A method according to claim 2, wherein said first, second and third supports comprise a porous membrane to which said antibodies are bound.
- 5. A method for determining the presence in a physiological sample of HLA-B27 antigen in the potential presence of HLA-B7 antigen, wherein a combination of monoclonal antibodies is used, a first antibody which is cross-reactive to both antigens, but not other common alleles of HLA antigens and a second antibody which binds to only said HLA-B7 of said HLA-B7 and B27 antigens, said method comprising:
- combining said sample with said second antibody bound to a solid support to form a liquid assay medium and depleting said HLA-B7 by separating said liquid assay medium from said solid support;
- combining said HLA-B7 depleted sample with three different monoclonal antibodies bound separately to three different solid supports comprising a porous membrane; a first support having an anti-first species antibody of a second species; a second support having said first antibody; and a third support having said second antibody;
- adding to said first, second and third supports a horse radish peroxidase enzyme conjugate of a first species antibody binding to HLA antigens, washing said supports, followed by adding a substrate producing a detectable product; and
- determining the presence of enzyme as to each of said first, second and third supports by means of said detectable product;
- wherein a negative value of said first support indicates the assay method failed;
- a positive value of said second support as compared to said third support indicates the presence of said HLA-B27 antigens.
- 6. A method for determining the presence in a physiological sample of a first antigen in the potential presence of a second antigen, where said first and second antigens are characterized by being members of a polymorphic group of alleles which share numerous epitopes, wherein a combination of monoclonal antibodies is used, a first antibody which is cross-reactive to both antigens, but not other common members of said group of alleles and a second antibody, which binds to only said second antigen of said first and second antigens, said method comprising:
- combining said sample, a negative control free of said members, and a positive control comprising said second antigen with first and second solid supports in separate assay media, said first support comprising said first antibody and said second support comprising said second antibody;
- washing said supports free of non-specifically bound components of said sample;
- adding an enzyme conjugate of an antibody binding to an epitope common to all members of said group of alleles with said first and second antigens, followed by addition of a substrate producing a detectable product;
- determining the amount of enzyme as to each of said assay media by means of said detectable product;
- wherein a value for said sample less than the value obtained for the negative control with the cross-reactive antibody plus a predetermined amount equal to about 10 to 30% of the value obtained for the positive control is negative for said first antigen;
- a value for said sample at least twice the quotient of the value for the positive control with said first antibody divided by the value for the positive control with said second antibody is positive for said first antigen.
- 7. A method according to claim 6, wherein said first antigens is HLA-B27 and said second antigen is HLA-B7.
- 8. A method according to claim 7, wherein said enzyme conjugate is a horse radish peroxidase conjugate with anti-.beta..sub.2 -microglobulin.
- 9. A membrane comprising at least three regions: a first region to which is bound a first monoclonal antibody cross-reactive with HLA-B7 and HLA-B27, but substantially not cross-reactive with other HLA antigens; a second region to which is bound a second monoclonal antibody reactive with HLA-B7, but not HLA-B27; and a third region to which is bound antibody specific for immunoglobulin from a predetermined species.
- 10. A kit comprising the membrane according to claim 9 and an enzyme conjugate of an antibody cross-reactive with HLA-B7 and HLA-B27.
- 11. A kit comprising an enzyme-monoclonal antibody conjugate binding to a common Class I HLA epitope; and
- a plurality of wells, one set or wells coated with a first monoclonal antibody binding to HLA-B27 and HLA-B7, but not other HLA alleles, a second set of wells coated with a second monoclonal antibody binding to HLA-B7, but not HLA-B27.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of application Ser. No. 07/745,163, filed Aug. 15, 1991 and now abandoned, which is a continuation-in-part of Ser. No. 07/698,319, filed May 10, 1991 now U.S. Pat. No. 5,256,543, issued Oct. 26, 1993.
US Referenced Citations (10)
Foreign Referenced Citations (2)
Number |
Date |
Country |
1684683 |
Oct 1991 |
SUX |
8705398 |
Sep 1987 |
WOX |
Non-Patent Literature Citations (4)
Entry |
Ferreira et al., Clin. Chim. Acta., 174:207-211 (1988). |
Sakaguchi et al., Human Immunol., 21:193-207 (1988). |
Trapani et al., Immunol. Cell Biol., 66:215-219 (1988). |
Villar et al., Eur. J. Immunol., 19:1835-1839 (1989). |
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
745163 |
Aug 1991 |
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Parent |
698319 |
May 1991 |
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