This application claims priority from Japanese Patent Application No. 2022-111426 filed in Japan on Jul. 11, 2022, and the entire disclosure of this application is hereby incorporated by reference.
The present disclosure relates to a hollow fiber membrane module suitable for use in the filtration process of culture or fermentation solutions to remove turbid components in water to be treated, for concentration and purification of target substances, or as a filter for ultra-pure water production, and a manufacturing method of the same.
When hollow fiber membranes are used in the process of manufacturing
peptide drugs, protein drugs, antibody drugs, and the like, utilizing E. coli, yeast, and animal cells, the batch culture method, in which unneeded components in the culture medium are removed by filtration after the culture is completed, and the continuous culture method, in which target substances are collected as needed during the culture, are used as appropriate. It is desired that these products be pre-sterilized and provided ready for use by the user with only minimal cleaning.
In order to suppress microbial growth in the product while maintaining filtration performance during storage, a glycerin solution, an alcohol solution, or a sodium hypochlorite solution has been used in typical hollow fiber membrane modules. In recent years, membrane modules filled with sterilized water are used for the purpose of reducing wastewater treatment of such storage liquids (see, for example, PTLs 1 and 2).
PTL 1: JP2018089614A
PTL 2: WO 2015/099015
Since the inside of the membrane module is filled with pure water in PTL 1, it is necessary to provide a mechanism to mitigate the pressure increase that occurs during the thermal treatment. In addition, since the inside of the membrane module is also filled with water in PTL 2, it is necessary to provide a mechanism to prevent the pressure from rising during the thermal treatment, and it is also necessary to dispose a closure member to prevent expanded water from coming into direct contact with aseptic connection members connected to the membrane module.
An object of the present disclosure is to solve the above problem and to provide a hollow fiber membrane module that can maintain filtration performance while preventing dryness of the hollow fiber membranes and can maintain asepticity inside a hollow fiber membrane module for a long period of time, and a manufacturing method of the same.
The present inventors have conducted intensive research and verification to satisfy the wide variety of requirements as described above. As a result, it was discovered that all requirements could be satisfied by sealing water and gas that have been sterilized in a certain ratio inside a hollow fiber membrane module, which led to the present disclosure. Specifically, the present disclosure is as follows:
[1]
A hollow fiber membrane module comprising:
a hollow fiber membrane accommodated in a module case, the hollow fiber being a plurality of hollow fiber membranes bundled together,
both end faces of the hollow fiber membrane bundle being integrated with the module case by a potting material,
wherein a storage liquid is contained in 90% or more of pores of the hollow fiber membrane bundle, the storage liquid being water free of viable bacteria,
a space portion inside the module case is filled with a gas free of viable bacteria and the storage liquid.
[2]
The hollow fiber membrane module according to [1], wherein
when a piping connection port present in the module case is sealed by a sealing member,
the following expression (1) is satisfied:
0.35Wall/Vall≤0.95 (1)
where Vfirst is a volume of a first space surrounded by an inner surface of a cap attached to the module case, an end face of the potting material, and inner surfaces and end faces at both ends of the hollow fiber membranes,
Vsecond is a volume of a second space surrounded by an inner surface of the module case, the end face of the potting material, and outer surfaces of the hollow fiber membranes,
Vpore is a volume of a third space formed by the pores of the hollow fiber membranes,
Vall is a total volume of Vfirst, Vsecond, and Vpore,
Wfirst is a volume of the storage liquid present in the first space,
Wsecond is a volume of the storage liquid present in the second space,
Wpore is a total volume of the storage liquid present in the third space, and
Wall is a total volume of Wfirst, Wsecond, and Wpore.
[3]
The hollow fiber membrane module according to [1] or [2], wherein
a ratio of Wfirst to Vfirst is equal to or less than a ratio of Wsecond to Vsecond.
[4]
The hollow fiber membrane module according to any one of [1] to [3], wherein
the ratio of Wsecond to Vsecond is 0.8 or more and 1 or less.
[5]
The hollow fiber membrane module according to any one of [1] to [4], further comprising
a pair of fixation portions made of a potting material that seals space between the outer surfaces of the hollow fiber membranes and between the outer surfaces and the inner surface of the tubular case at both ends of the hollow fiber membrane bundle inside the module case, and a pair of flow guide cylinders that are provided closer to a longitudinal center side of the module case than the pair of fixation portions, and are disposed so as to surround respective ends of the hollow fiber membrane bundle,
wherein a separation distance between one ends of the flow guide cylinders located on fixation portion sides and the fixation portions is 1 mm or more and 20 mm or less.
[6]
The hollow fiber membrane module according to any one of [1] to [5], wherein
TOC in the storage liquid contained in the module case is 1 ppm or more and 50 ppm or less.
[7]
The hollow fiber membrane module according to any one of [1] to [6], wherein
all piping connection ports provided to the module case are sealed by blind caps,
a linear expansion coefficient of a material used for the blind caps is between 0.95 and 1.05 times a linear expansion coefficient of the module case.
[8]
The hollow fiber membrane module according to [1],
wrapped by a wrapping film having a first portion, at least part of which has gas permeability.
[9]
The hollow fiber membrane module according to [8], wherein
the module case is cylindrical, and at least a part of the piping connection port provided to the module case protrudes in a direction perpendicular to a longitudinal direction of the module case,
the hollow fiber membrane module is wrapped by the wrapping film so that a second portion of the wrapping film, which has a smaller gas permeability than the first portion, is positioned within a range of 120° centered around a direction rotated by 90° relative to a direction of protrusion of the port or within a range of 60° centered around an opposite direction of the direction of protrusion, in a radial direction of an axis of the module case.
[10]
The hollow fiber membrane module according to any one of [1] to [9], wherein
an aseptic connector is attached to the piping connection port provided to the module case,
the aseptic connector comprises a sterile filter, and
one end face of the sterile filter is in direct contact with a space inside the hollow fiber membrane module.
[11]
A manufacturing method of a hollow fiber membrane module comprising:
a hollow fiber membrane inserted in a module case, the hollow fiber being a plurality of hollow fiber membranes bundled together,
both end faces of the hollow fiber membrane bundle being integrated with the module case by a potting material,
wherein a storage liquid is filled in 90% or more of pores of the hollow fiber membrane bundle, the storage liquid being water free of viable bacteria,
a space portion inside the module case is filled with a gas free of viable bacteria and the storage liquid, the method comprising:
when a connection piping portion present in the module case is sealed by a blind cap, adjusting Wall inside the hollow fiber membrane module so that the following expression (1) is satisfied:
0.35≤Wall/Vall≤0.95 (1),
where Vfirst is a volume of a first space surrounded by an inner surface of a cap attached to the module case, an end face of the potting material, and inner surfaces and end faces at both ends of the hollow fiber membranes, Vsecond is a volume of a second space surrounded by an inner surface of a pipe portion of the module case, the end face of the potting material, and outer surfaces of the hollow fiber membranes, Vpore is a volume of a third space formed by the pores of the hollow fiber membranes, Vall is a total volume of Vfirst, Vsecond, and Vpore, Wfirst is a volume of water present in the first space, Wsecond a volume of water present in the second space, Wpore second is a total volume of water present in the third space, and Wall is a total volume of Wfirst, Wsecond, and Wpore,
transition the hollow fiber membrane module to a state where no viable bacteria are present in water and a gas by thermally treating at 80° C. to 125° C. while a piping connection port is sealed.
[12]
The manufacturing method of a hollow fiber membrane module according to [11], wherein
a relative humidity inside the module case is maintained to 85% or higher in the step of thermally treating the hollow fiber membrane module in which the pure water and the gas are sealed.
[13]
The manufacturing method of a hollow fiber membrane module according to or [12], comprising
heating an outside of the hollow fiber membrane module by dry air in the step of thermally treating the hollow fiber membrane module in which the pure water and the gas are sealed.
[14]
The manufacturing method of a hollow fiber membrane module according to any one of to [13], wherein
in the hollow fiber membrane module in which an aseptic connector is attached to the piping connection port provided to the module case, a pressure difference between a pressure inside the module case and a pressure outside the module case at 20° C. before start of the thermal treatment is 0 kPa, and
the pressure difference between the pressure inside the module case and the pressure outside the module case separated by a sterile filter of the aseptic connector during the thermal treatment is 20 kPa or less.
[15]
The manufacturing method of a hollow fiber membrane module according to any one of to [14], wherein
in the step of thermally treating the module case having the water and the gas sealed therein,
the thermal treatment is carried out while the piping connection port in the module case is sealed by a blind cap.
[16]
The manufacturing method of a hollow fiber membrane module according to any one of to [15], wherein
the thermal treatment is carried out while the hollow fiber membrane module is wrapped by a wrapping film having a first portion, at least part of which has gas permeability.
[17]
The manufacturing method of a hollow fiber membrane module according to [16], wherein
the module case is cylindrical, and at least a part of the piping connection port provided to the module case protrudes in a direction perpendicular to a longitudinal direction of the module case,
in the step of thermally treating the hollow fiber membrane module having the water and the gas sealed therein, the hollow fiber membrane module is wrapped by the wrapping film so that a second portion of the wrapping film, which has a smaller gas permeability than the first portion, is positioned within a range of 120° centered around a direction rotated by 90° relative to a direction of protrusion of the port or within a range of 60° centered around an opposite direction of the direction of protrusion, in a radial direction of an axis of the module case.
[18]
A cleaning method of a hollow fiber membrane module, wherein
when an inside of a hollow fiber membrane module manufactured by the manufacturing method according to any one of [11] to [17] is cleaned by filtration cleaning,
an TOC of filtrated water becomes 500 ppb or less by filtrating pure water at 20 L/m2 or less per membrane area of the hollow fiber membranes.
According to the present disclosure, because water free of viable bacteria as the storage liquid for the membrane module to maintain filtration performance and a gas free of viable bacteria are contained at an appropriate ratio, the hollow fiber membrane module in a sterilized state can be provided while maintaining the filtration performance of the membranes.
Gamma ray sterilization or electron beam irradiation sterilization can be selected as a treatment for the hollow fiber membrane module prior to use. However, even in such cases, the environment inside the hollow fiber membrane module prior to irradiation can be maintained in a constant state, there is therefore no need to adjust the irradiation dose each time and sterilization can be achieved with a minimum amount of irradiation.
In addition, after the filtration module is attached in an apparatus, filtration operation can be started after minimum rinsing.
In the accompanying drawings:
Hereinafter, a hollow fiber membrane module employing one embodiment of a hollow fiber membrane module of the present disclosure will be described with reference to the drawings.
A hollow fiber membrane module of the present embodiment can be used for cell separation after culture in biopharmaceutical production, and for purification and concentration of biopharmaceuticals. The hollow fiber membrane module of the present embodiment can be used for internal pressure filtration. Hollow fiber membrane modules are required to have high filtration performance in order to reduce the sizes of facilities, and the hollow fiber membrane module of the present embodiment can be a hollow fiber membrane module that allows for a higher filtration flow rate per unit volume.
As illustrated in
Piping connection caps 10 and 11 are provided at the openings at both ends of the case 5, and conduits 10a and 11a to which piping are to be connected are formed to the caps 10 and 11. The piping connection caps 10 and 11 are fixedly attached to the case 5 by nuts 13. The nuts 13 are screwed into male threads formed in the side surfaces of the both ends of the case 5. By tightening the nuts 13, O-rings 12 placed in grooves in the caps 10, 11 seal between the both ends of the case 5 and the caps 10, 11.
Upper and lower nozzles 5a and 5b are formed at the both end portions of the case 5, through which fluid is to flow. The upper and lower nozzles 5a and 5b are provided so as to protrude in the direction perpendicular to the longitudinal direction of the case 5.
At both end faces of the hollow fiber membrane bundle 3, each hollow fiber membrane 3a is open. At the both end faces, the hollow fiber membranes 3a are bonded together by a potting material to form fixation portions 14. The both ends of the hollow fiber membrane bundle 3 are integrated by the potting material.
In the internal pressure filtration technique, for example, a liquid is introduced through the cap 11, the liquid flows into hollow portions of the opened hollow fiber membranes, the liquid permeates from the inner surface of each hollow fiber membrane 3a between the fixation portions 14 at the both ends, and the liquid that has passed to the outer surface of each hollow fiber membrane flows out through the upper nozzle 5a, for example. In external pressure filtration, for example, a liquid is introduced through the lower nozzle the liquid permeates from the outer surface of each hollow fiber membrane 3a between the fixation portions 14 at the both ends, and the liquid that has passed through the hollow portion of each hollow fiber membrane 3a flows out of through conduits 10a, 11a of the cap 10, 11.
Microfiltration membranes, ultrafiltration membranes, or the like can be used as the hollow fiber membranes 3a . The material of the hollow fiber membranes is not limited. Example thereof include polysulfone, polyethersulfone, polyacrylonitrile, polyimide, polyetherimide, polyamide, polyetherketone, polyetheretherketone, polyethylene, polypropylene, poly(4-methylpentene), ethylene-vinyl alcohol copolymer, cellulose, cellulose acetate, polyvinylidene fluoride, and ethylene-tetrafluoroethylene copolymer, and polytetrafluoroethylene, and composite materials of these can also be used.
The inner diameter of the hollow fiber membranes 3a is 50 μm to 3000 μm, preferably 50 μm to 2000 μm. The inner diameter of the hollow fiber membranes 3a is preferably 50 μm or more because a small inner diameter increases the pressure loss and adversely affects filtration. In addition, the inner diameter is preferably 3000 μm or less because it is difficult to maintain the shape of the membrane during the manufacturing of the fibers in a configuration with a large inner diameter.
Polymeric materials, such as epoxy resins, vinylester resins, urethane resins, unsaturated polyester resins, olefinic polymers, silicone resins, and fluorine-containing resins are preferred as the potting material, and any of these polymeric materials may be used or a combination of multiple polymeric materials may be used.
Note that, in the manufacturing process of biopharmaceuticals and the production of ultra-pure water for semiconductor applications, the components are required to have high heat resistance to hot water during cleaning and to have low elution characteristic. Therefore, the material of the hollow fiber membranes 3a is preferably a material with low elution, such as polyvinylidene fluoride, polysulfone, polyethersulfone, and polyphenylsulfone. The material of the case 5 is also preferably a material with low elution, such as polysulfone-based materials. For the same reason, an epoxy resin is preferably used as the potting material.
In addition, in the hollow fiber membrane module 1 of the present embodiment, an appropriate amount of water free of viable bacteria serving as a storage liquid is sealed in the space inside the case. Specifically, the storage liquid is contained in 90% or more of the pores of the hollow fiber membrane bundle 3 in the hollow fiber membrane module 1. The storage liquid is a liquid for maintaining the filtration performance of the hollow fiber membranes 3a. Water free of viable bacteria is water defined as follows. A sample is collected from the hollow fiber membrane module and incubated for 48 to 72 hours at 25 to 35° C. in a total count sampler available from Merck Millipore, Inc. If no colony is observed visually and under a magnifying microscope at a magnification of about 20×, the water is confirmed to be water free of viable bacteria. The internal space other than the space occupied by the storage liquid is occupied by a gas free of viable bacteria. Therefore, the hollow fiber membrane module is turned ten times up and down in the longitudinal direction of the hollow fiber membrane module while the hollow fiber membrane module is sealed with blind caps, and a sampled water is incubated with a total count sampler available from Merck Millipore for 48 to 72 hours at 25 to 35° C. If no colony is observed visually and under a magnifying microscope at a magnification of about 20×, the water and the gas are confirmed to be free of viable bacteria. It is desirable to use a gas from which particulates have been removed in advance by a HEPA filter or other means. Air may be used as the gas, or a nitrogen atmosphere may be used to further reduce oxidative degradation of the components used in the hollow fiber membrane module during thermal sterilization. After the storage liquid and the gas are filled, the water and the gas may then be sterilized by thermal sterilization.
Here, the space that is present inside the hollow fiber membrane module will be described. The space inside the hollow fiber membrane module 1 is classified into the following three areas, when the piping connection ports are sealed by sealing members. Specifically, the piping connection ports are the upper nozzle 5a, the lower nozzle 5b, and the conduits 10a, 11a. Specifically, the sealing members are blind caps, which will be discussed below. As illustrated in
Here, Wall/Vall preferably 0.35 or more and 0.95 or less. If Wall/Vall is smaller than 0.35, the storage liquid contained in the pores of the hollow fiber membranes 3a may volatilize during thermal sterilization, and the expected filtration performance may not be achieved. In addition, if Wall/Vall is smaller than 0.35, bubbles may be generated during a leak test conducted prior to filtration operation, which may be misinterpreted as the occurrence of a leak in the hollow fiber membranes. If Wall /Vall is greater than 0.95, the internal pressure of the case 5 during thermal sterilization will be increased, which may affect the durability of the product.
When the water content ratio in the pores of the hollow fiber membranes 3a is defined as Wpore/Vpore, Wpore/Vporeis 0.9 or more, preferably 0.98 or more even after thermal sterilization.
Furthermore, when the water content ratio on the primary side of the hollow fiber membrane module is defined as Wfirst/Vfirst and the water content ratio on the secondary side is defined as Wsecond/Vsecond, the relationship between the water content amount preferably satisfies: Wfirst/Vfirst≤Wsecond/Vsecond. If Wfirst/Vfirst≤Wsecond/Vsecond is satisfied, a larger proportion of water is contained in the space Vsecond which is preferentially heated during the thermal sterilization treatment. Accordingly, this process can be performed while preventing dryness of the hollow fiber membranes. On the other hand, When Wfirst/Vfirst is kept to be smaller than Wsecond/Vsecond, water expanded by the thermal sterilization treatment moves from the outer surface side to the inner surface side through the pores of the hollow fiber membranes 3a, which mitigates the internal pressure increase in the case 5. In addition, by keeping Wfirst/Vfirst smaller, the hollow fiber membrane module 1 after filling with the storage liquid can be made lighter, which is advantageous from the viewpoint of transportation.
Furthermore, the water content ratio Wsecond/Vsecond of the second space sp2 is preferably 0.8 or more and 1 or less. If the water content ratio Wsecond/Vsecond is 0.8 or more, the temporary decrease in the relative humidity of the second space sp2 during the temperature increase in the thermal sterilization treatment can be suppressed and the process time can be shortened. Furthermore, although it depends on the filling ratio of hollow fiber membranes 3a relative to the inner diameter of the case 5 before the thermal sterilization treatment, if the water content ratio Wsecond/Vsecond is 0.8 or more, the outer surface of the case 5 can be made to be immersed with water free of viable bacteria when the case is placed horizontally in the axial direction. Therefore, the occurrence of drying during thermal sterilization can be further reduced. If the water content ratio Wsecond/Vsecond is 0.8 or more, vibration of the hollow fiber membranes 3a caused by vibration of bubbles during transportation of the hollow fiber membrane module 1 is suppressed, and the possibility of damage can be reduced.
In the hollow fiber membrane module 1, respective flow guide cylinders 19a, 19b are disposed between the upper and lower nozzles 5a and 5b and the hollow fiber membranes 3a. The flow guide cylinders 19a, 19b may be cylinders coaxial with the inner circumference of the case 5. One ends of the flow guide cylinders 19a, 19b and the fixation portions 14 are preferably separated from each other. There are two types of the hollow fiber membrane module 1 used in biopharmaceutical manufacturing applications: single-use type modules that are disposed of after one-time use, and modules that are repeatedly used after a cleaning operation. In the case of the repeated use type, the hollow fiber membrane module 1 is subjected to a history of thermal cycles due to sterilization by means of hot water or steam at 80° C. or higher, and the configuration in which parts of the flow guide cylinders 19a, 19b are embedded in the fixation portions 14, a crack starting from the fixation portions 14 may be generated. On the other hand, when the flow guide cylinders 19a, 19b are separated from the fixation portions 14 as in the above configuration, the product can be used for a longer period of time while being remained in good condition. The separation distance in the axial direction of the case 5 is preferably between 1 mm and 20 mm, although it varies depending on the location. If the separation distance is less than 1 mm, there is a possibility that the flow guide cylinders 19a, 19b and the fixation portions 14 may contact in a part of areas, depending on the method to apply an adhesive. In contrast, in the configuration where the separation distance is 20 mm or more, a filtrate may flow in or a cleaning solution used during cleaning may flow in due to backwash cleaning from the separated areas, which increases the local flow rate and imparts a load on the roots of the hollow fiber membranes 3a, in other words, the area near the fixation portions 14.
In addition, in the hollow fiber membrane module 1 of the present embodiment, the content of organic matter in the storage liquid as TOC (Total Organic Carbon) is preferably 1 ppm or more and less than 50 ppm. If the TOC is 1 ppm or more, the organic matter in the water is preferentially oxidized during thermal sterilization of water to be described below, and the oxidative degradation of the hollow fiber membranes 3a due to heating can be suppressed. Note that the TOC is more preferably 5 ppm or more.
If the TOC is less than 50 ppm, it is possible to quickly reduce the concentration of organic matter in the storage liquid at the start of use of the hollow fiber membrane module 1, as well as reducing the time required for rinsing before starting operation and the amount of a rinsing solution.
Furthermore, in the hollow fiber membrane module 1 of the present embodiment, the concentration of metal ions in contained the storage liquid is preferably 10 ppb or more and less than 100 ppb. In the biopharmaceutical process, contamination with metal ions that adversely affect the pharmaceuticals produced should be avoided, and a lower content is thus more preferred. On the other hand, when sterilization is taken into consideration, metal ions are known to exhibit sterilizing effects. Thus, it is possible to improve the sterilizing effects by heating through inclusion of metal ions to the extent that they do not adversely affect the manufacturing of pharmaceuticals and to the extent that rinsing can be performed easily.
The hollow fiber membrane module 1 of the present embodiment described above can suppress the growth of bacteria during storage while providing filtration performance that satisfies the requirements when the hollow fiber membrane module 1 is used in biopharmaceutical applications. Note that the hollow fiber membrane module 1 of the present embodiment can be used as a final filter for ultra-pure water production used in semiconductor manufacturing applications, enabling rapid reduction of the concentration of organic matter in the storage liquid while also achieving filtration performance.
All of the upper nozzle 5a, the lower nozzle 5b, and the conduits 10a, 11a, which are piping connection ports provided in the case 5, are preferably sealed by blind caps 15, 16, 17, and 18, respectively, as illustrated in
Aseptic connectors 20 may be used as blind caps for sealing the nozzle portions of the case 5, as illustrated in
The hollow fiber membrane module 1 can be wrapped in a wrapping film made of a plastic, and stored and transported to prevent water evaporation during storage. Low-density polyethylene, high-density polyethylene, polypropylene, polyvinylidene chloride, and the like are suitably used as the film material for the wrapping film. Or, when a thermal sterilization treatment using an autoclave is performed on the hollow fiber membrane module 1 which has been wrapped, the wrapping film may be a sterile bag. A sterile bag is one that has a porous first portion that prevents bacteria from entering from the outside to the inside of the bag while allowing water vapor to permeate freely. The first portion has gas permeability. Alternatively, the wrapping film may be formed by bonding the outer edges of a film having a first portion and a film having a second portion made of high-density polyethylene, polypropylene, or the like. The second portion has smaller gas permeability than the first portion.
The total weight of the hollow fiber membrane module 1 of the present disclosure, including the storage liquid, can be 15 kg or more. In addition, the hollow fiber membrane module 1 has portions, such as the corners of the nuts 13, where loads tend to concentrate when the hollow fiber membrane module 1 is placed horizontally in the longitudinal direction. If the first portion of the wrapping film is disposed between such a portion and the floor surface during installation, the pores of the first portion may be crushed, which may compromise the durability of the wrapping film. Therefore, it is preferable to provide the first portion outside the area of the wrapping film that is in contact with the area of the hollow fiber membrane module 1 where the load is likely to be concentrated. In other words, it is preferable to provide a second portion in the area of the wrapping film that is in contact with the area of the hollow fiber membrane module 1 where the load is likely to be concentrated.
As illustrated in
Alternatively, the hollow fiber membrane module 1 wrapped with the wrapping film 23 may be wrapped so that the porous portion 22 are sandwiched between the hollow fiber membrane module 1 and the stationary portion 24, as long as the compressive strength generated in the porous portion 22 is 10 kPa or less when the hollow fiber membrane module 1 is placed on the stationary portion 24. For example, the compressive strength to the porous portion 22 can be reduced by placing the hollow fiber membrane module 1 so that the load is not concentrated on the porous portion 22, but is distributed to other areas.
The hollow fiber membrane module 1 of the present embodiment described above can prevent the thick portions of the hollow fiber membranes 3a from drying during the thermal sterilization treatment, prevent bacteria growth during storage, and prevent damage to the hollow fiber membranes 3a due to vibration during transportation, while satisfying the water quality requirements required in the biomedical field.
Next, a manufacturing method of the hollow fiber membrane module 1 of the present embodiment will be described.
In a manufacturing method of the hollow fiber membrane module 1 of the present embodiment, water sterilized by filtration and a gas are first introduced in the case 5 of the hollow fiber membrane module 1 in the state illustrated in
Here, pure water in the present disclosure refers to water from which ionic components are reduced, and which has an electrical conductivity of 1 μS/cm or less and has been filtrated through a reverse osmosis membrane or ultrafiltration membrane. The number of particles of 50 nm or larger contained in pure water is preferably 10 particles/L or more and 200 particles/L or less.
In addition, it is preferable that water having the organic content expressed by TOC of 1 ppm or more and 50 ppm or less in the pure water is used. Furthermore, the concentration of metal ions contained in pure water is preferably 10 ppb or more and less than 100 ppb, and the concentration of chloride ions contained in pure water is preferably 25 ppb or more and less than 250 ppb.
In the manufacturing method of the hollow fiber membrane module 1 of the present embodiment, the hollow fiber membrane module 1 (case 5), to which water sterilized by filtration is sealed, is then thermally treated at 80° C. or higher and 125° C. or lower. Thus, the hollow fiber membrane module 1 can be transitioned to a state where the water and the gas inside are free of viable bacteria by further heat sterilizing the water which has been sterilized by filtration along with the gas.
Although addition of a chemical agent is one method of sterilizing water, in the case of hollow fiber membrane modules used in the biopharmaceutical field, it is preferable to sterilize water by heating without adding any unnecessary component because the cleaning operation before filtration operation requires time and pure water for cleaning, and the wastewater from the cleaning process also increases.
When water is sterilized by heating, as in the present embodiment, even if the temperature is below 80° C., a lot of viable bacteria are killed by carrying out heating over a long period of time. However, in view of long-term storage of three months or longer, it is desirable to heat the water to 80° C. or higher to sufficiently kill bacteria. On the other hand, if the heating temperature is 125°C. or higher, components may be damaged due to the difference in thermal expansion coefficients of the components. In view of these, the temperature of the thermal treatment is preferably 80° C. or higher and 125° C. or lower, as in the present embodiment. In addition, when the hollow fiber membrane module 1 of the present embodiment is further sterilized by gamma rays after the thermal treatment, the inside of the hollow fiber membrane module 1 can always be kept in the same condition by performing a thermal sterilization treatment at 85° C. or higher to 95° C. or lower before gamma ray irradiation, and sterilization can be achieved with a minimum gamma ray irradiation.
In cases where the hollow fiber membrane module 1 is thermally treated as described above, if the space inside the case 5 is full of water (in other words, if Vall=Wall), the thermal expansion of the water causes a pressure increase inside the module, which might damage the hollow fiber membranes 3a and the case 5. One method to alleviate this pressure increase is to make the inside and outside of the module communicate to each other. However, in this case, water that has expanded in volume due to the thermal treatment would overflow to the outside, and the volume contraction upon cooling might cause the intake of outside air, which might lead to the introduction of bacteria from the atmosphere into the module. In addition, after cooling, blind caps for the piping connection ports of the hollow fiber membrane module 1 must be replaced with new ones for final storage and transportation purposes, which would require a cumbersome operation.
To avoid these issues, in the manufacturing method of the hollow fiber membrane module 1 of the present embodiment, a gas is preferably contained in at least one of the first space sp1, the second space sp2, and the third space sp3 of the hollow fiber membrane module 1 at a certain ratio so that the pressure increase can be mitigated even if the water filled as the storage liquid expands due to heating.
In addition, in the step of thermally treating the hollow fiber membrane module 1 to which water and the gas have been sealed, the relative humidity of the gas inside the hollow fiber membrane module 1 is constantly preferably 85% or more, more preferably 90% or more to prevent dryness of the hollow fiber membranes 3a. If the relative humidity is below 85%, the hollow fiber membrane 3a may become dry depending on the total time during which the relative humidity is below the limit. In particular, dryness may occur in the hollow fiber membranes 3a located at the outermost periphery of the hollow fiber membrane bundle 3. As a manufacturing method in which the relative humidity is kept to a certain level or higher, heating may be divided into multiple stages and the temperature may be increased in stages. Or, since the temperature of the second space sp2 inside the hollow fiber membrane module 1 rises first upon heating, it is desirable that the water content ratio in the second space sp2 is high. Or, the outermost hollow fiber membranes 3a which are relatively distant from the cluster of the hollow fiber membrane bundle 3 tend to become dry more easily. As such, the hollow fiber membrane bundle 3 may be constrained with a net-like constraint member to prevent a part of the hollow fiber membranes 3a from separating from the cluster of the other hollow fiber membranes 3a.
The method to heat the hollow fiber membrane module 1 can be selected as appropriate, but the hollow fiber membrane module 1 can be heated from outside thereof with dry air, moist heat, or pressurized steam. Microwaves may also be used. During heating, the hollow fiber membrane module 1 may be placed horizontally in the longitudinal direction during heating, or it may be held approximately perpendicularly to the ground, or thermal treatment may be performed while the hollow fiber membrane module 1 is rotated around the longitudinal axis of the hollow fiber membrane module 1.
It is also preferable that blinds caps are attached to the piping connection ports in the hollow fiber membrane module 1 prior to the start of the thermal treatment, in other words, the thermal sterilization treatment. Alternatively, aseptic connectors 20 may be attached. Sterile filters 21 that are attached to the aseptic connectors 20 for preventing the influx of bacteria, so that the connection ports are sealed. To prevent the sterile filters 21 from peeling off due to an increase in the internal pressure of the hollow fiber membrane module 1, the pressure difference between the inside and outside of the hollow fiber membrane module 1 before the start of the thermal treatment is preferably 0 kPa at an external temperature of 20° C., and the pressure difference between the inside and outside of the hollow fiber membrane module 1 during the thermal treatment is preferably within 20 kPa, more preferably within 10 kPa. The thermal treatment described above is preferably performed while the piping connection ports are sealed by blind caps.
Next, one example of an aspect in which the hollow fiber membrane module 1 of the present embodiment is installed in a filtration apparatus 100 for filtration for biopharmaceuticals will be described with reference to FIG. 10, and a rinsing method and a filtration method using the hollow fiber membrane module 1 of the present embodiment will be further explained. It is assumed that the cross-flow filtration technique by means of internal pressure filtration is used in this filtration treatment apparatus 100 for filtration for biopharmaceuticals.
As illustrated in
In the filtration apparatus 100, the water to be treated is supplied from the treatment water tank 106 through the supply piping 104 and the lower conduit 11a to the hollow portion, i.e., the insides of the hollow fiber membranes 3a. The supplied water to be treated is filtrated to the outer surface sides of the hollow fiber membranes 3a, and the filtrate is collected from the upper nozzle 5a while the lower nozzle 5b of the case 5 is closed. For example, the lower nozzle 5b is closed by closing a valve 102a, which is connected to the lower nozzle 5b via the second filtrated water collection piping 102, which will be described below. Most of the supplied water to be treated is discharged as circulating water from the upper conduit 10a the hollow fiber membrane bundle 3 and returned to the treatment water tank 106 through the circulation piping 105.
When the hollow fiber membrane module 1 is installed in the filtration apparatus 100 described above, the blind caps 15, 16, 17, and 18 for sealing the hollow fiber membrane module 1, which are one of the embodiments, are opened, and the storage liquid sealed inside the hollow fiber membrane module 1 is then discharged to the piping outside the water treatment apparatus 100. The hollow membrane module 1 is then attached to the piping of the filtration apparatus 100. Upon installation, the hollow fiber membrane module 1 may be installed to the water treatment apparatus 100 after the storage liquid in the hollow fiber membrane module 1 is discharged outside the system.
Alternatively, the aseptic connectors 20 for sealing the hollow fiber membrane module 1, which are another embodiment, may be connected to aseptic connectors attached to the piping of the filtration apparatus 100 and the sterile filters 21 are peeled off, which allows for connection while maintaining the asepticity inside the hollow fiber membrane module 100 and the piping of the filtration apparatus 100.
Here, a cleaning method of the hollow fiber membrane module 1 before it is used in a filtration operation will be described. After the hollow fiber membrane module 1 is attached to the filtration apparatus 100, pure water is introduced into the hollow portion (primary side) of the hollow fiber membranes 3a of the hollow fiber membrane module 1 at a predetermined pressure from the supply piping 104 through the lower conduit 11a. In the hollow portion, most of the pure water is filtrated through the hollow fiber membranes 3a and moves to the outer surface side (secondary side) of the hollow fiber membranes 3a. The filtrated pure water is then drained from the upper nozzle 5a of the hollow fiber membrane module 1. Alternatively, the drained water (filtrated pure water) may be drained from the lower nozzle 5b of the hollow fiber membrane module. In addition, after 5 L/m2 or 10 L/m2 of water per membrane area of the hollow fiber membrane module 1 has been filtrated, all pure water present on the outer surface side of the hollow fibers (secondary side) may be drained through the lower nozzle 5b of the hollow fiber membrane module, and then pure water is introduced again from the lower cap 11a. In this manner, the inside of the hollow fiber membrane module 1 can be cleaned more efficiently. The TOC on the filtrated water side can be kept 500 ppb or lower after filtration is performed with 20 L/m2 or less of pure water per membrane area of the hollow fiber membrane module 1.
The present embodiment will now be described in more details with reference to examples and comparative examples, yet it is noted that the present embodiment is not limited to these examples.
In the following examples and comparative examples, hollow fiber membrane modules were used. The characteristics thereof and measurement methods are as follows.
Water sealed in a hollow fiber membrane module was sampled and whether or not viable bacteria were present or not was determined using a HPC total count sampler manufactured by Millipore (model: MHPC10025).
The TOC in the storage liquid was analyzed using the following instrument.
Linear expansion coefficients of module cases and blind caps were measured according to the thermo-mechanical analysis method of JIS K7197-1991.
A temperature sensor was inserted in the center of a hollow fiber membrane module in the longitudinal direction. The temperature sensor was disposed so that the temperature at the center of the hollow fiber membrane bundle was measured. The measurement apparatus used was TR-7wb manufactured by T&D Corporation, and a stainless steel protection tube sensor (TR-1220) was used as the temperature sensor element.
A thermo-hygrometer (LR8514) manufactured by HIOKI E. E. CORPORATION was used for measurements of the relative humidity. Holes for wiring were drilled in advance in blind caps for sealing the nozzles and blind caps for sealing the conduits. After the wiring was set, the module was sealed with an epoxy resin and the relative humidity inside the hollow fiber membrane module was measured.
After completion of the sterilization treatment and storage for a specified period of time, whether leak was present or not and dryness was present or not were determine by the following procedure. First, after the water inside the hollow fiber membrane module was drained, the conduits located on the primary side of the hollow fiber membrane module and one nozzle located on the secondary side of the hollow fiber membrane module were sealed with blind caps. Air of up to 0.2 MPa was then pumped from the other nozzle on the secondary side to check whether or not air bubbles were generated from the openings of the hollow fiber membranes at the end faces in the fixation portion. Samples without bubbles at this point were determined to be free of both leak and dryness. On the other hand, if bubbles were observed, the same leak test was performed again after hydrophilization treatment was performed with alcohol. If bubbles were no longer generated at this time, it was determined that dryness had occurred. If bubbles continued to be observed after the hydrophilization treatment, the hollow fiber membrane was determined to have a leak.
The water permeation amount through the hollow fiber membrane module at 25° C. and 0.1 MPa was calculated by introducing pure water from the inner surface side of the hollow fiber membranes, i.e., the primary side of the module, and measuring the amount of pure water permeating through to the outer surface of the hollow fiber membranes, i.e., the secondary side. The retention ratio of water permeation amount through the module was calculated from the ratio of the water permeation amounts through the hollow fiber membrane module before thermal sterilization and through the hollow fiber membrane module stored according to the three-month storage test after a thermal sterilization treatment described below.
After the water in the hydrophilized hollow fiber membrane module was completely drained, the weight of the hollow fiber membrane module was measured. The hollow fiber membrane module was dried in an environment of 50° C. until there was no weight loss, and weighed again. The water content amount and the water content ratio of the pores of the hollow fiber membranes were calculated from the difference between the two.
After the inside of the hydrophilic hollow fiber membrane module was filled with pure water, the water present in the primary and secondary sides was drained. The maximum water content amounts were calculated, which was used as the respective volumes of spaces. The water content amount in each example was determined as the water content amount in the first space and the second space by feeding metered amounts of water into each space. The water content ratio was calculated as the ratio of the maximum water content amount to the water fed into each space. Here, the primary side of the hollow fiber membrane module refers to the first space, and the secondary side of the hollow fiber membrane module refers to the second space. If water has been already contained in the primary and secondary sides of the hollow fiber membrane module, the respective water content amount can be calculated by the following procedure. To measure the water content amount in the primary side, all ports (top and bottom nozzles, and top and bottom conduits) are sealed with blind caps in advance. While the hollow fiber membrane module is placed so that the longitudinal direction thereof is substantially vertical to the ground, the blind caps on the conduits on the upper and lower sides of the primary side are opened. After the module is allowed to stand for 5 minutes, the water drained from the lower side of the conduit is collected. The water content amount of the primary side, in other words, the first space, can be determined from the volume of water drained at that time. To measure the water content amount in the secondary side, all ports (upper and lower nozzles, and upper and lower conduits) are sealed with blind caps in advance. While the hollow fiber membrane module is placed so that the longitudinal direction thereof is substantially vertical to the ground, the blind caps of the upper and lower nozzles are opened. Water drained from the lower nozzle is collected for 5 minutes. The water content amount of the secondary side space can be determined from the volume of water drained at that time.
A porous hollow fiber membrane was thinly sliced in a cross section perpendicular to the longitudinal direction using a razor and the outer and inner diameters were measured with a magnifying glass of a magnification of 100×.
For one sample, measurements were made at 60 cross-sections at 30 mm intervals in the length direction, and the average values were taken as the outer and inner diameters of the hollow fiber membrane.
Hollow fiber membrane modules after thermal sterilization were stored in an environment of room temperature for three months. As used therein, room temperature refers to temperatures 18° C. or higher and 25° C. or lower. Thereafter, the method of determining the presence or absence of leak and presence or absence of dryness of hollow fiber membranes described above were performed on the hollow fiber membrane modules after the storage period to determine the presence or absence of leak and dryness. The above-mentioned method for checking the sterilization effect was performed on hollow fiber membrane modules after the storage period to determine whether or not viable bacteria grew.
Hollow fiber membrane modules after thermal sterilization treatment were placed horizontally in the longitudinal direction inside a dryer. After the hollow fiber membrane modules were heated under the setting of 50° C. for 48 hours, they were left exposed to the atmosphere for 24 hours and allowed to stand at room temperature (20 to 24° C.). This temperature cycle was repeated the required number of times. The above method to determine presence or absence of dryness of hollow fiber membranes was performed on hollow fiber membrane modules subjected to 10, 40, and 70 cycles of temperature cycling to determine presence or absence of dryness of hollow fiber membranes.
The following test were conducted on hollow fiber membrane modules packed in a cardboard box used for packing. The corrugated cardboard box was placed on a test platform and a packing box having a weight of 100 kg was stacked on the top of the cardboard box. Vibrations were applied while the frequency was varied in the frequency range of 1 Hz to 200 Hz and the power spectral density (PSD) was changed as needed from 0.000004 g2/Hz to 0.02 g2/Hz, such that the total vibration load applied was 0.53 overall Grms. Here, PSD is a numerical value representing the intensities of waves per frequency in random vibration. In this random vibration test, the hollow fiber membrane modules were subjected to vibration loading according to the random vibration test procedure (Over the Road Trailer Spectrum) of ISTA 3A. Furthermore, the packing box containing the hollow fiber membrane module was subjected to a vibration test history where no load was applied from the top of the packing box. Vibrations were varied in the frequency range of 1 Hz to 200 Hz and the PSD level was changed between 0.0005 g2/Hz and 0.035 g2/Hz. The test was conducted in accordance with the random vibration test procedure (Pick-up and Delivery Vehicle Spectrum) of ISTA 3A and a vibration load applied amounted to a total PSD of 0.46 overall Grms. In all tests, a test apparatus (model: G9250-L) manufactured by Shinken Co. Ltd. was used. All of the tests were carried out in an environment of 20 to 24° C. The leak determination method for hollow fiber membranes described above was applied to the hollow fiber membrane modules subjected to the vibration test to determine the presence or absence of leak.
After a hollow fiber membrane module which had been subjected to the thermal sterilization treatment was installed in the apparatus, cold water (maximum 25° C.) and hot water (maximum 75° C.) were introduced to the module alternately every 30 minutes. After completion of the target number of cycles, the membrane module was inspected to determine absence or presence of leak using the leak detection method described above.
After the hollow fiber membrane module which had been subjected to the thermal sterilization treatment was installed in the filtration process apparatus, pure water was introduced from the lower side of the hollow fiber membrane module cap. The rinse operation was performed by adjusting the circulation flow rate to 1 L/min and the filtration flow rate to 10 L/min. Every minutes after the start of rinsing, pure water was sampled from the upper side of the cap of the hollow fiber membrane module and from the filtrated water side. The TOC of the sampled pure water was analyzed using the same apparatus used for analyses of the storage liquid. The elution amount from the hollow fiber membrane module was measured by measuring the TOC. The filtration amount when the TOC of pure water on the filtration water side became less than 500 ppb as the target value of filtration water quality was measured.
Deionized water that had been filtrated through an ultrafiltration membrane in advance was filled from the primary side to the secondary side of a hollow fiber membrane module provided with a microfiltration membrane made of PVDF, and then the hollow fiber membrane module was allowed to stand still in the longitudinal direction perpendicular to the ground for 5 minutes. After the water inside was completely drained off, a predetermined amount of pure water was fed to the primary and secondary sides of the hollow fiber membrane module so that the water content ratios in the first space and the second space were adjusted to the water content ratios listed in Table 1. All piping connection ports were then sealed by clamping using blind caps made of polysulfone. The module was then placed horizontally in a dryer and thermal treatment was carried out at 90° C. for 24 hours, followed by slow cooling so that the sterilized water and gas were sealed inside the hollow fiber membrane module. Various storage and durability tests were conducted on a hollow fiber membrane module that had been sterilized. The results are as summarized in Table 1. After three months of storage at room temperature, a leak test was performed and it was confirmed that neither leak nor dryness of membranes occurred. The module water permeation amount was measured, and the retention rate compared to that before thermal sterilization was 99%, indicating that the product remained in good condition. Deionized water was collected from inside the hollow fiber membrane module and the presence or absence of viable bacteria was examined, and it was confirmed that no viable bacteria were present. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 16 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., no dryness of the hollow fiber membranes occurred after completion of cycles, but dryness of the hollow fiber membranes was confirmed after completion of 40 cycles. The location of dryness was at the outermost periphery of the hollow fiber membrane bundle. In addition, after the thermal sterilization was completed, a leak inspection was performed after a hollow fiber membrane module was subjected to vibration. It was confirmed that no leak occurred and the hollow fiber membranes were not damaged. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 1, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.05 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.81. The hollow fiber membrane module in Example 2, in which a heat sterilized product was stored at room temperature for three months, was confirmed to have neither dryness nor leak of the hollow fiber membranes. In addition, the module water permeability retention rate was 101%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 15 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the thermal sterilization was completed, a leak inspection was performed after a hollow fiber membrane module was subjected to vibration. It was confirmed that no leak occurred and the hollow fiber membranes were not damaged. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 2, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.07 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.9. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 100%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 15 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the thermal sterilization was completed, a leak inspection was performed after a hollow fiber membrane module was subjected to vibration. It was confirmed that no leak occurred and the hollow fiber membranes were not damaged. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 3, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.28 and the pure water content ratio in the second space was set to Wsecond/Vsecond =0.98. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 103%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 15 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the thermal sterilization was completed, a leak inspection was performed after a hollow fiber membrane module was subjected to vibration. It was confirmed that no leak occurred and the hollow fiber membranes were not damaged. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 4, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.65 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.96. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 99%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 14 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the thermal sterilization was completed, a leak inspection was performed after a hollow fiber membrane module was subjected to vibration. It was confirmed that no leak occurred and the hollow fiber membranes were not damaged. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 5, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.92 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.95. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 99%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 14 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the thermal sterilization was completed, a leak inspection was performed after a hollow fiber membrane module was subjected to vibration. It was confirmed that no leak occurred and the hollow fiber membranes were not damaged. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 6, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.07 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.08. The heat sterilized product was stored at room temperature for 3 months, and bubbles were observed to be generated from one location of the hollow fiber membranes. After the hydrophilization treatment was applied again, a leak test was performed and no bubbles were observed, indicating that the hollow fiber membranes had dryness. In addition, the module water permeability retention rate was 98%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 18 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C. a leak test was carried out after completion of 10 cycles, and bubbles were observed to be generated from the hollow fiber membranes in three locations. Then, after the hydrophilization treatment was applied again, a leak test was performed and no bubbles were observed, indicating that the hollow fiber membranes had dryness.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The difference was as follows: hollow fiber membrane modules were subjected to a three-month storage test at room temperature without a history of thermal sterilization. After three months of storage at room temperature, a leak test was performed and no bubbles were observed. In addition, the module water permeability retention rate was 99%, which was a good result. The presence or absence of viable bacteria was inspected and the presence of bacteria was confirmed. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 18 L/m2 of pure water per membrane area.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=1.00 and the pure water content ratio in the second space was set to Wsecond/Vsecond=1.00. When the piping connection ports were sealed with blind caps and heat treatment was performed at a set temperature of 90° C., the internal pressure of the hollow fiber membrane module increased to 560 kPa, causing the housing to be subjected to a relatively high pressure hi story.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.47 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.53. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 97%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 16 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. On the other hand, leak inspection was conducted after vibration was applied to the hollow fiber membrane module after the thermal sterilization had been completed, and occurrence of leakages from two locations were confirmed. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 7, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.28 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.31. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 99%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 17 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. After the completion of thermal sterilization, a leak inspection was performed after the hollow fiber membrane module was subjected to vibration, and no bubbles were observed, confirming that no leaks had occurred. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 8, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.98 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.08. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 98%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 16 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., leak inspection was conducted after completion of 10 cycles and no bubbles were observed and no leakage occurred. However, after 40 and 70 cycles were completed, it was confirmed that dryness had occurred in the hollow fiber membranes when leak inspection was conducted. After the completion of thermal sterilization, a leak inspection was performed after the hollow fiber membrane module was subjected to vibration, and no bubbles were observed, confirming that no leaks had occurred. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 9, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.98 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.49. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 100%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 16 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., a leak test was carried out after 10, 40, and 70 cycles were completed, and no bubbles were generated from the hollow fiber membranes, confirming that no leaks occurred. On the other hand, after applying vibration to the hollow fiber membrane module after the thermal sterilization had been completed, a leak inspection was conducted and confirmed that bubbles were generated from the hollow fiber membrane at one location, indicating that a leak had occurred. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 10, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
Deionized water that had been filtrated through an ultrafiltration membrane in advance was filled from the primary side to the secondary side of a hollow fiber membrane module provided with a microfiltration membrane made of polysulfone, and then the hollow fiber membrane module was allowed to stand still in the longitudinal direction perpendicular to the ground for 5 minutes. After the water inside was completely drained off, a predetermined amount of pure water was fed to the primary side of the hollow fiber membrane module so that the pure water content ratio in the first space was set to Wfirst/Vfirst=0.07 and the pure water content ratio in the secondary side space was set to Wsecond/Vsecond=0.99. All piping connection ports were then sealed by clamping using blind caps made of polysulfone. The module was then placed horizontally in a dryer and thermal treatment was carried out at 90° C. for 24 hours, followed by slow cooling so that the sterilized water and gas were sealed inside the hollow fiber membrane module Various storage and durability tests were conducted on a hollow fiber membrane module that had been sterilized. The results are as summarized in Table 3. After three months of storage at room temperature, a leak test was performed and it was confirmed that neither leak nor dryness of membranes occurred. The module water permeation amount was measured, and the retention ratio compared to that before the thermal sterilization was 102%, indicating that the product remained in good condition. Deionized water was collected from inside the hollow fiber membrane module and the presence or absence of viable bacteria was examined, and it was confirmed that no viable bacteria were present. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 15 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the membrane module was subjected to vibration after thermal sterilization was completed, a leak test was conducted. It was confirmed that no leakage occurred and that the hollow fiber membrane was not damaged. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. At the completion of 500 cycles, there were no leaks or other defects. However, a leak inspection was conducted after 700 cycles, and a leak was confirmed at one location. After the module was disassembled to check the leak location, the starting point was found to be where the flow guide cylinder was buried in the fixation portion.
Hollow fiber membrane modules the same as hollow fiber membrane modules in Example 11 were used. The difference was as follows: the pure water content ratio in the first space of the hollow fiber membrane module is set to Wfirst/Vfirst=0.53. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 101%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 14 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the membrane module was subjected to vibration after thermal sterilization was completed, a leak test was conducted. It was confirmed that no leakage occurred and that the hollow fiber membrane was not damaged.
Hollow fiber membrane modules the same as hollow fiber membrane modules in Example 11 were used. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.88 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.95. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 99%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 13 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the membrane module was subjected to vibration after thermal sterilization was completed, a leak test was conducted. It was confirmed that no leakage occurred and that the hollow fiber membrane was not damaged.
Hollow fiber membrane modules the same as hollow fiber membrane modules in Example 11 were used. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.97 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.07. After a thermally sterilized product was stored at room temperature for three months, it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred. In addition, the module water permeability retention rate was 97%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 17 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., no leaks were observed when leak inspection was conducted after completion of 10 cycles. However, when leak inspections were conducted after and 70 cycles were completed, it was confirmed that the hollow fiber membranes had dryness. In addition, after the membrane module was subjected to vibration after thermal sterilization was completed, a leak test was conducted. It was confirmed that no leakage occurred and that the hollow fiber membrane was not damaged.
Hollow fiber membrane modules the same as hollow fiber membrane modules in Example 11 were used. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.07 and the pure water content ratio in the second space was set to Wsecond/Vsecond=0.06. After a thermally sterilized product was stored at room temperature for three months, the hollow fiber membranes were confirmed to have dryness. In addition, the module water permeability retention ratio was 90%, which was lower than that before the thermal sterilization by 10%. Viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 18 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., a leak test was carried out after 10, 40, and 70 cycles were completed, and the hollow fiber membranes were confirmed to have dryness in the all tests.
Hollow fiber membrane modules the same as hollow fiber membrane modules in Example 11 were used. The difference was as follows: the storage liquid for the hollow fiber membrane module was changed from pure water to a 65 wt % glycerin solution. The content ratio of the glycerin solution in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.05, and the content ratio of the glycerin solution in the second space was set to Wsecond/Vsecond=0.04. After a thermally sterilized product was stored at room temperature for three months, the module water permeability retention ratio was 101%, which was within a good range. No viable organisms were observed. A rinse test was conducted after installation to a filtration apparatus, and 200 L/m2 of pure water per membrane area was required before the TOC on the filtrated water side fell below 500 ppb.
Hollow fiber membrane modules having the same configuration as in Example 1 were used for the tests. The differences were as follows: the pure water content ratio in the first space of the hollow fiber membrane module was set to Wfirst/Vfirst=0.12, the pure water content ratio in the second space was set to Wsecond/Vsecond=0.18, and aseptic connectors were used. Aseptic connectors (AQS33012HT) manufactured by CPC were used as aseptic connectors. In addition, the hollow fiber membrane modules in Example 15 were packed in sterile bags (product name: cleanpeak Easy-Tear Bag) prior to thermal treatment. One side of the sterile bag was made of a non-woven fabric (Tybek 1073b) with excellent water vapor permeability, and the opposite side was made of a high-density polyethylene film. The module was placed in the thermal treatment apparatus so that the high-density polyethylene film was placed in a portion sandwiched between the hollow fiber membrane module and the floor of the thermal treatment apparatus and the nonwoven fabric surface was placed above the hollow fiber membrane module. This was done to prevent excessive compressive force on the nonwoven fabric surface and prevent collapse of the pores in the nonwoven fabric. Steam sterilization treatment at 125° C. was then performed. The treated product was stored at room temperature for three months and it was confirmed that neither dryness nor leak of the hollow fiber membrane occurred In addition, the module water permeability retention rate was 98%, which was a good result. No viable bacteria were found. A rinse test was conducted after installation to a filtration apparatus, and the TOC on the filtrated water side reached below 500 ppb with the use of only 17 L/m2 of pure water per membrane area. For a hollow fiber membrane module stored in an environment of temperature cycle of 20° C. to 50° C., it was confirmed that no dryness of the hollow fiber membranes occurred after completion of any of 10, 40, or 70 cycles. In addition, after the membrane module was subjected to vibration after thermal sterilization was completed, a leak test was conducted. It was confirmed that no leakage occurred and that the hollow fiber membrane was not damaged. In addition, a cool and heat cycle test was conducted on a hollow fiber membrane module after the thermal sterilization had been completed. In Example 15, hollow fiber membrane modules having a configuration in which the fixation portions and flow guide cylinders were separated were also used. After 500 cycles were completed, a leak test was performed and no leakage was observed.
1 Hollow fiber membrane module
3 Hollow fiber membrane bundle
3
a Hollow fiber membrane
5 Module case
5
a Upper nozzle
5
b Lower nozzle
10, 11 Cap
10
a,11aConduit
12 O-ring
13 Nut
14 Fixation portion
15,16,17,18 Blind cap
19
a,19b Flow guide cylinder
20 Aseptic connector
21 Sterile filter
22 Second portion
23 Wrapping film
24 First portion
25 Stationary portion
100 Filtration apparatus
101 First filtrated water collection pipe
102 Second filtrated water collection pipe
101
a First valve
102
a Second valve
104 Feed piping
105 Circulation piping
106 Treatment water tank
sp1 First space
sp2 Second space
sp3 Third space
YY First blind cap
ZZ Second blind cap
Number | Date | Country | Kind |
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2022-111426 | Jul 2022 | JP | national |