BACKGROUND OF THE INVENTION
M. tuberculosis infects approximately one third of the world's population, resulting in 3 million deaths annually. Cegielski, J. P., et al. Infect Dis Clin North Am 16:1-58 (2002). Soon after inhalation of tubercle bacilli, the organisms are phagocytosed by alveolar macrophages, resulting in potent cell-mediated immune responses and the formation of granulomas, which consist primarily of T cells and M. tuberculosis-infected macrophages. Flynn, J. L., and J. Chan. Infect Immun 69:4195-4201 (2001); Kaplan, G., et al. Infect Immun 71:7099-7108 (2003). Six to eight weeks after infection in humans, and coincident with the development of a delayed-type hypersensitivity response manifested by tuberculin skin test positivity, these granulomas undergo caseous necrosis, resulting in the death of the majority of tubercle bacilli and destruction of surrounding host tissue. Grosset, J. Antimicrob Agents Chemother 47:833-836 (2003). The small proportion of surviving bacilli are thought to exist in a nonreplicating hypometabolic state, as an adaptation to the unfavorable milieu in the solid caseous material. Id. This altered physiologic state, termed latent tuberculosis infection, can endure for the lifetime of the infected individual, but in approximately 10% of cases, through unknown mechanisms, these dormant bacilli reactivate many years to decades later to produce disease.
Efforts to gain insight into the adaptive mechanisms by which M. tuberculosis persists in the host have been impeded by the inability to recover sufficient quantities of M. tuberculosis RNA from host lesions consistent with contained latent tuberculosis infection. Talaat, A. M., et al. Proc Natl Acad Sci USA 101:4602-4607 (2004). Consequently, several groups have turned to in vitro models which may reflect the persistent state, and have defined the gene expression profile of M. tuberculosis under conditions of hypoxia (Sherman, D. R., et al. Proc Natl Acad Sci USA 98:7534-7539 (2001), Rosenkrands, I., et al. J Bacteriol 184:3485-3491 (2002)), nutrient starvation (Betts, J. C., et al. Mol Microbiol 43:717-731 (2002)), low pH (Fisher, M. A., et al. J Bacteriol 184:4025-4032 (2002)), low concentrations of nitric oxide (Voskuil, M. I., et al. J Exp Med 198:705-713 (2003)), and in the phagosomal compartment of murine macrophages (Schnappinger, D., et al. J Exp Med 198:693-704 (2003)). Current work has focused on the role of the two-component response regulator dormancy survival regulator (dosR), which initially was found to be the primary mediator of the hypoxic response in M. tuberculosis (Sherman, supra, Park, H. D., et al. Mol Microbiol 48:833-843 (2003)). Bacilli exposed to low, nontoxic concentrations of nitric oxide in vitro enter a nonreplicating persistent state marked by the induction of a 48-gene regulon under the control of dosR, suggesting that the dosR regulon may mediate the transition of these bacilli into dormancy. Voskuil, M. I., et al. J Exp Med 198:705-713 (2003). Consistent with these findings, several dosR regulon genes, including acr (Rv2031c), Rv2623c, and Rv2626, are upregulated in infected mouse tissues after the onset of Th1 immunity. Voskuil, supra, Shi, L., et al. Proc Natl Acad Sci USA 100:241-246 (2003).
Because evaluation of cellular changes in vivo is typically difficult, if not impossible, the results of the studies of latent tuberculosis infection discussed above have been limited to in vitro models. Thus, there remains a need for a fast, effective in vivo technique to investigate changes in defined populations of prokaryotic and eukaryotic cells within an animal.
SUMMARY OF THE INVENTION
The present invention is directed to a method of using hollow fibers to evaluate cellular changes in vivo. The hollow fiber technique can be used to study the behavior of microorganisms or other cells of interest under various conditions in animals, such as, for example, in response to a specific drug or drugs of interest. Thus, a method for evaluating cellular changes in vivo in response to administration of a drug or drugs of interest is provided. In another embodiment, the hollow fiber technique is used to evaluate cellular changes in a microorganism in vivo. In one embodiment, the technique is used to evaluate cellular changes in a microorganism in vivo in response to administration of a drug or drugs of interest. A vaccine and a method of vaccinating an animal are also provided.
Other systems, methods, features, and advantages of the present invention will be or become apparent to one with skill in the art upon examination of the following drawings and detailed description. It is intended that all such additional systems, methods, features, and advantages be included within this description and be within the scope of the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS
Many aspects of the invention can be better understood with reference to the following drawings.
FIG. 1 is a photograph of an SKH1 mouse with a subcutaneously-implanted hollow fiber containing M. tuberculosis.
FIG. 2 is a graph of the reduced growth of bacilli within hollow fibers in vivo. Colony-forming unit (CFU) counts per fiber of hollow fiber-encapsulated M. tuberculosis implanted into mice (HF in vivo) are compared to those of hollow fiber-encapsulated M. tuberculosis incubated in vitro (HF in vitro).
FIG. 3 is a photograph of hollow-fiber encapsulated bacilli that remain viable in vivo. As a control, in vitro-grown cultures of M. tuberculosis H37Rv-lux were treated with 70% ethanol for 3 hours in order to promote bacillary death (FIG. 3a). Live bacilli exhibit green fluorescence while dead bacilli fluoresce red. Approximately half of all in vivo hollow fiber-encapsulated organisms on days 21 (FIG. 3b) and 28 (FIG. 3c) after fiber implantation were determined to be viable based on their staining properties.
FIG. 4 is a graph of the reduced metabolic activity of encapsulated bacilli in vivo. FIG. 4a is a graph of the relationship of relative light units (RLU) to colony-forming units (CFU) in mid-log phase M. tuberculosis H37Rv-lux grown in vitro. FIG. 4b is a graph of luciferase activity of hollow fiber-encapsulated M. tuberculosis implanted into mice (HF in vivo) vs. hollow fiber-encapsulated M. tuberculosis incubated in vitro (HF in vitro).
FIG. 5 is a graph demonstrating that hollow fiber-encapsulated bacilli are more susceptible to rifampin than to isoniazid. The activities of isoniazid 0.05% in the diet (INH) and rifampin 0.02% in the diet (RIF) against hollow fiber-encapsulated bacilli in vivo are compared to no treatment (Control).
FIG. 6 is a photograph of the formation of granuloma-like lesions surrounding M. tuberculosis-containing hollow fibers. Gross skin lesions surrounding hollow fibers containing liquid broth alone at day 1 (FIG. 6a), day 14 (FIG. 6b), and day 28 (FIG. 6c), and those containing M. tuberculosis H37Rv-lux at day 1 (FIG. 6d), day 14 (FIG. 6e), and day 28 (FIG. 6f), following hollow fiber implantation. Histopathology of tissues surrounding hollow fibers containing liquid broth is in FIGS. 6g-6i and those containing M. tuberculosis H37Rv-lux is shown in FIGS. 6j-6l) 28 days after hollow fiber implantation (hematoxylin-eosin stain). Arrows indicate hollow fiber membrane.
FIG. 7 is a graph demonstrating that containment of intra-fiber bacillary growth in vivo is immune-mediated and IFNγ-dependent. Mice implanted with hollow fibers containing M. tuberculosis (HF+M. tb) developed enlarged spleens as early as 14 days after implantation, as compared to mice implanted with fibers containing media (HF control) (FIG. 7a). Wild type Balb-C/J (WT) mice were able to contain the growth of hollow fiber-encapsulated M. tuberculosis to a greater extent than isogenic IFNγ-deficient (IFNg−/−) mice 28 days after hollow fiber implantation (FIG. 7b).
FIG. 8 is a graph of the absence of relMtb-deficient mutant by PCR from a pool of mutants after 21 days of cultivation within mouse granulomas. PCR amplification of the transposon insertion junction reveals presence of the relMtb::Tn mutant in both input (Day 1) and output (Day 21) pools in hollow fibers incubated in vitro (FIG. 8a) but absence of the mutant in the output pool (Day 21) in mouse-implanted hollow fibers (FIG. 8b), suggesting reduced survival of this mutant in vivo. 1=Rv1347 (hypothetical transcriptional regulator); 2 =Rv0250 (miscellaneous oxidoreductase); 3=Rv2583 (relMtb); 4=Rv1069 (pra). Hollow fiber-encapsulated wild-type M. tuberculosis CDC 1551 (WT) and relMtb::Tn mutant (RelMtb) grow equally when incubated in vitro (FIG. 8c), but the latter strain demonstrates significantly reduced survival when hollow fibers are implanted into mice (FIG. 8d).
FIG. 9 is a photograph of the colony size of hollow fiber-encapsulated M. tuberculosis incubated in vitro (left) versus implanted in mice (right) for 21 days. Photograph was obtained 17 days after plating.
FIG. 10 is a graph of the activity of moxifloxacin (MXF) against hollow fiber-encapsulated bacilli implanted into mice, as compared with no treatment (control).
DETAILED DESCRIPTION
The present invention is directed to a method of using hollow fibers to evaluate cellular changes in vivo. The hollow fiber technique involving the use of semi-diffusible hollow fibers can be used to study the behavior of encapsulated microorganisms or other cells of interest under various conditions in animals.
The hollow fiber technique provides a unique method to study the behavior of a pure population of prokaryotic or eukaryotic cells in response to various conditions in an animal. In one embodiment, this technique is used to evaluate cellular changes in vivo in response to administration of a drug or drugs of interest. In one embodiment, the cells employed are eukaryotic cells such as human cells that are evaluated for potential toxicity or activity in response to administration of a drug or drugs of interest. In another embodiment, the hollow fiber technique is used to evaluate cellular changes in microorganisms in vivo, such as, e.g., during latency. In one embodiment, the technique is used to evaluate cellular changes in a microorganism in vivo in response to administration of a drug or drugs of interest. In another embodiment cellular changes of defined populations of prokaryotic or eukaryotic cells in the conditions described above including latency, exposure to drugs, exposure to immunomodulators, or exposure to gene therapy vectors may be monitored in animals that are genetically engineered with deficiencies or altered gene expression of specific animal genes. Thus, this hollow fiber technique can be used to evaluate such cellular changes as those developed during latent tuberculosis infection and can be used to characterize the human cellular pharmacogenomic expression profiles of various drugs against different human cell types.
In one embodiment, this technique is used to evaluate cellular changes in vivo in response to administration of a drug or drugs of interest. In one embodiment, the cells are evaluated for potential toxicity or activity in response to administration of a drug or drugs of interest. In one embodiment, this assay is used to screen novel drugs for desired activity or undesired toxicity.
Any suitable cells or cell lines can be evaluated, including, but not limited to, differentiated human cells. In one embodiment, the cells are non-transformed cells. In another embodiment, the cells are non-transformed cells isolated from a human. For example, in one embodiment, a drug or drugs are screened for potential toxicity against human cell types, for example, stem cells, peripheral blood cells (including peripheral blood mononuclear cells), lymphoid cells, hepatocytes, bone marrow-derived cells, skin biopsies, broncho-alveolar lavage washings, breast tissue cells, kidney cells, oral, urethral, vaginal, cervical, or gastric, or intestinal mucosal cells or mucosal biopsies, reproductive cells (ova or spermatocytes), adipose cells, nerve or stromal cells, bone or synovial cells, or other suitable human cell types, by studying the transcriptional profiles of these cells. It is envisioned that both normal and malignant cells from the tissues mentioned will be amenable to hollow-fiber testing. Global gene expression patterns of various human cell types with the use of microarrays, reverse transcriptase-polymerase chain reaction (RT-PCR), or other gene expression methods are correlated with specific cellular toxicity profiles. In one embodiment, different profiles are associated with cells isolated from different patients, which could allow for individualized medicine. In another embodiment, novel chemotherapeutic agents are tested for activity against specific human cancer cell lines, by quantification of cells prior to and following administration of the drug. In addition, the same drugs are then screened using the hollow fiber technique for potential toxicity against other human cell types. Therefore, the hollow fiber technique provides a rapid, inexpensive in vivo assay with which to screen promising new drugs for human activity and toxicity parameters prior to the investment of significant resources in human clinical trials.
In one embodiment, a method for evaluating cellular changes in vivo in response to administration of a drug or drugs of interest is provided. The method comprises encapsulating cells of interest in a hollow fiber; implanting the hollow fiber into an animal; administering the drug or drugs of interest to the animal; isolating the cells from the hollow fiber; and evaluating the transcriptional profiles of the cells. The animal can be any suitable animal, such as, for example, a mouse, a rat, a guinea pig, a rabbit, a sheep, a pig, a cow, a chicken, or a dog, or genetically engineered variant animals of the aforementioned species. Of course, more than one animal can be utilized. The cells can be any suitable cells, such as those described herein, such as, e.g., cells isolated from a human. The transcriptional profiles can be evaluated in any suitable manner, such as those known in the art, for example, by using microarray analysis. In one embodiment, the transcriptional profiles are correlated with specific cellular toxicity or activation profiles. In this embodiment, the correlation allows a determination of the effects of the administration of the drug or drugs on the cells.
The drug or drugs administered can be any suitable drug or combination of drugs, including, e.g., novel drugs and known drugs. The drug can be administered in any suitable manner or using any suitable dosing schedule, such as, e.g., once a day, twice a day, three times a day, bi-weekly, three times a week, four times a week, or any other suitable dosing schedule. If more than one drug is administered, they can be administered concurrently or sequentially or at different times or even different days altogether. In one embodiment, the drug or drugs is selected from the group consisting of: analgesics, anesthetics, anti-acne agents, antibiotics, anticholinergics, anticoagulants, anticonvulsants, antidiabetic agents, antidyskinetics, antifibrotic agents, antifungal agents, anti-glaucoma agents, anti-infectives, anti-inflammatory compounds, antimicrobial compounds, antineoplastics, antiParkinsonian agents, antirheumatic agents, antiosteoporotics, antiseptics, antisporatics, antithrombotics, antivirals, appetitite stimulants, bacteriostatics, biologicals, blood modifiers, bone metabolism regulators, calcium regulators, cardioprotective agents, cardiovascular agents, central nervous system stimulants, cholinesterase inhibitors, contraceptives, cystic fibrosis agents, deodorants, detoxifying agnets, diagnostics, disinfectants, dietary supplements, dopamine receptor agonists, enzymes, erectile dysfunction agents, fertility agents, gastrointestinal agents, gout agents, hormones, hypnotics, immunomodulators, immunosuppressives, keratolytics, mast cell stabilizers, migraine agents, motion sickness agents, multiple sclerosis treatments, muscle relaxants, nasal preparations, nucleoside analogs, obesity agents, opthalmic agents, osteoporosis agents, parasympatholytics, parasympathomimetics, prostaglandins, psychotherapeutic agents, respiratory agents, sclerosing and anti-sclerosing agents, sedatives, skin and mucous membrane agents, smoking cessation agents, sympatholytics, ultraviolet screening agents, urinary tract agents, vaginal agents, vasodilators, or combinations thereof. Suitable classes of drugs are described in, e.g., Physicians' Desk Reference, 56th Ed., Medical Economics Company, Inc., Montvale, N.J., pages 201-202 (2002). In one embodiment, the drug is a novel drug with unknown or uncertain activity and/or toxicity.
The drug or drugs can be administered for any period of time suitable to give the desired result. In one embodiment, the drug or drugs is administered for a period of from about 1 hour to about 30 days, such as, e.g., about 1 hour, about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 7 hours, about 8 hours, about 9 hours, about 10 hours, about 11 hours, about 12 hours, about 13 hours, about 14 hours, about 15 hours, about 16 hours, about 17 hours, about 18 hours, about 19 hours, about 20 hours, about 21 hours, about 22 hours, about 23 hours, about 1 day, about 2 days, about 3 days, about 4 days, about 5 days, about 6 days, about 7 days, about 8 days, about 9 days, about 10 days, about 11 days, about 12 days, about 13 days, about 14 days, about 15 days, about 16 days, about 17 days, about 18 days, about 19 days, about 20 days, about 21 days, about 22 days, about 23 days, about 24 days, about 25 days, about 26 days, about 27 days, about 28 days, about 29 days, and about 30 days. Of course, a suitable amount of time could also include more than about 30 days where appropriate.
In another embodiment, the hollow fiber technique is used to evaluate cellular changes in a microorganism in vivo, such as, e.g., during latency. In one embodiment, a method for evaluating cellular changes in a microorganism in vivo is provided. The method comprises: encapsulating one or more microorganisms in a hollow fiber; implanting the hollow fiber into an animal; isolating the microorganisms from the hollow fiber; and evaluating the transcriptional profiles of the microorganisms. Any suitable animal can be employed, such as, e.g., those described herein. The microorganisms can isolated from the hollow fiber after any period of time suitable for evaluating the cellular changes, such as, e.g., from about 1 hour to about 30 days, such as, e.g., about 1 hour, about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 7 hours, about 8 hours, about 9 hours, about 10 hours, about 11 hours, about 12 hours, about 13 hours, about 14 hours, about 15 hours, about 16 hours, about 17 hours, about 18 hours, about 19 hours, about 20 hours, about 21 hours, about 22 hours, about 23 hours, about 1 day, about 2 days, about 3 days, about 4 days, about 5 days, about 6 days, about 7 days, about 8 days, about 9 days, about 10 days, about 11 days, about 12 days, about 13 days, about 14 days, about 15 days, about 16 days, about 17 days, about 18 days, about 19 days, about 20 days, about 21 days, about 22 days, about 23 days, about 24 days, about 25 days, about 26 days, about 27 days, about 28 days, about 29 days, and about 30 days. Of course, a suitable amount of time could also include more than about 30 days where appropriate.
The transcriptional profiles can be evaluated in any suitable manner, such as those known in the art, for example, by using microarray analysis. In one embodiment, the transcriptional profiles are used to design drugs that specifically target the microorganism. For example, in one embodiment, the method allows identification of genes required for persistence that can be specifically targeted in the design of drugs or vaccines. In one embodiment, such drugs or vaccines can be screened against the microorganism using the hollow fiber technique. In one embodiment, the microorganism is deficient in a specific gene, such as, e.g., a gene required for persistence.
Any suitable microorganism can be evaluated. In one embodiment, the microorganism is selected from the group consisting of: Mycobacterium species including M. tuberculosis, Staphylococcal species including Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, other pathogenic Streptococcal species, including Enterococci, Haemophilus species, Moraxella species, Neisseria species, Legionella species, Listeria species, Chlamydia species, Mycoplasma species, Pseudomonas species, Escherichia coli, Klebsiella species, Enterobacter species, Serratia species, Acinetobacter species, Xanthomonas species, Stenotrophomonas, Borrelia species, Treponemal species, Nocardia species, Actinomycete species, Bacteroides species, Clostridial species including C. difficile, Peptostreptococci, Bacillus species, Francisella species, Yersinia species, Candida species, including Candida albicans, Histoplasma species, Cryptococcus species, Aspergillus species, Blastomycosis species, viruses within appropriate cellular carriers including HIV, smallpox virus (Variola), hepatitis A, B, C, D, and E viruses, influenza viruses, rhinoviruses, adenoviruses, coxsackie viruses, parainfluenza viruses, poliovirus, measles virus, Varicella virus, Herpesviruses including HSV-1 and HSV-2, Cytomegalovirus (CMV), Noroviruses, and parasitic species including Plasmodia species, Giardia species, Toxoplasma species, Schistosoma species, Trypanosoma species, and Leishmania species. In one embodiment, the hollow fiber technique is used to evaluate microorganisms other than M. tuberculosis, such as, e.g., aerobic and anaerobic bacteria, fungi and yeasts, parasites, and virus carried within appropriate defined cells.
In one embodiment, the technique is used to evaluate cellular changes in a microorganism in vivo in response to administration of a drug or drugs of interest. In one embodiment, a method for evaluating cellular changes in a microorganism in vivo in response to administration of a drug or drugs of interest, comprising: encapsulating one or more microorganisms in a hollow fiber; implanting the hollow fiber into an animal; administering the drug or drugs of interest to the animal; isolating the microorganisms from the hollow fiber; and evaluating the transcriptional profiles of the microorganisms. The animal can be any suitable animal, such as, for example, those described herein. The microorganism can be any suitable microorganism, such as those described herein. The transcriptional profiles can be evaluated in any suitable manner, such as those known in the art, for example, by using microarray analysis. In one embodiment, the transcriptional profiles are correlated with specific cellular toxicity or activation profiles. In this embodiment, the correlation allows a determination of the effects of the administration of the drug or drugs on the microorganism. In one embodiment, the microorganism is a microorganism other than M. tuberculosis, such as, e.g., such as, e.g., aerobic and anaerobic bacteria, fungi and yeasts, parasites, and virus carried within appropriate defined cells.
The drug or drugs administered can be any suitable drug or combination of drugs, such as, e.g, those described herein. In one embodiment, the drug or drugs can be an antibacterial antibiotic, an antiviral agent, antiparasitic agent, or anti-fungal agent or combination thereof.
The drug or drugs can be administered for any period of time suitable to give the desired result. In one embodiment, the drug or drugs is administered for a period of from about 1 hour to about 30 days, such as, e.g., about 1 hour, about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 7 hours, about 8 hours, about 9 hours, about 10 hours, about 11 hours, about 12 hours, about 13 hours, about 14 hours, about 15 hours, about 16 hours, about 17 hours, about 18 hours, about 19 hours, about 20 hours, about 21 hours, about 22 hours, about 23 hours, about 1 day, about 2 days, about 3 days, about 4 days, about 5 days, about 6 days, about 7 days, about 8 days, about 9 days, about 10 days, about 11 days, about 12 days, about 13 days, about 14 days, about 15 days, about 16 days, about 17 days, about 18 days, about 19 days, about 20 days, about 21 days, about 22 days, about 23 days, about 24 days, about 25 days, about 26 days, about 27 days, about 28 days, about 29 days, and about 30 days. Of course, a suitable amount of time could also include more than about 30 days where appropriate.
In one embodiment, this technique is used to study the transcriptional profile of Mycobacterium tuberculosis during latency using microarray analysis, in order to identify upregulated genes whose products may be involved in metabolic pathways critical for mycobacterial survival and persistence within the host. This information provides the basis for rational design of drugs to specifically target the persistent stage of tuberculosis infection. M. tuberculosis mutants deficient in specific genes are tested individually or as members of a pool for the ability to persist in the hollow fiber mouse model. Genes required for persistence are specifically targeted in the rational design of attenuated vaccine strains. Finally, promising new anti-TB drugs are screened in the hollow fiber model of latent TB infection for activity against slowly-replicating, metabolically hypoactive (i.e., dormant) M. tuberculosis.
The hollow fiber technique is additionally useful for: screening drugs as antibiotics against M. tuberculosis; screening drugs as antibiotics against other bacteria, such as, for example, bacteria that cause osteomyelitis, or foreign-body (e.g., catheters, prostheses, etc.) infections, e.g., Staph. aureus, Enterococci, fungi, such as, e.g., Candida albicans; assessing the transcriptional and proteomic profile of bacteria within the fibers (“latent-like”) bacteria, a novel approach to vaccination (the bacteria appear to secrete small molecules outside of the fibers which elicit immune responses); or screening encapsulated human cells implanted in mice for transcriptional signatures associated with drug efficacy or drug toxicity.
Therefore, in one embodiment, a vaccine is provided. The vaccine comprises at least one hollow fiber comprising at least one microorganism. Any suitable microorganism can be utilized, such as, e.g., those set forth herein. In one embodiment, the microorganism is attenuated. In one embodiment, the microorganism is Mycobacterium tuberculosis. In another embodiment, the microorganism is mutant M. tuberculosis. Any suitable attenuated microorganism can be used, such as, e.g., microorganisms deficient in genes for persistence.
A method of vaccinating an animal is also provided. The method comprises encapsulating one or more microorganisms in a hollow fiber and implanting the hollow fiber into the animal. Any suitable animal can be vaccinated, such as, e.g., a human. In one embodiment, the microorganism is attenuated. In one embodiment, the microorganism is Mycobacterium tuberculosis. In another embodiment, the microorganism is mutant M. tuberculosis. Any suitable attenuated microorganism can be used, such as, e.g., microorganisms deficient in genes for persistence.
In one embodiment, the vaccine is prepared by encapsulating one or more microorganisms into at least one hollow fiber. The vaccine can be prepared using any suitable microorganism, including vaccines that are attenuated and/or mutated. Attenuation and/or mutation of the microorganism can be accomplished using any suitable techniques, such as, e.g., those described herein or otherwise known in the art.
The hollow fiber technique provides a rapid screening tool to test the activity of novel chemotherapeutic agents against encapsulated organisms or other cells of interest in animals. This technique can also be used to study the global transcriptional profile of encapsulated organisms or other cells of interest within animals. In addition, the hollow fiber technique can be used to study the ability of various bacterial mutants to persist within animals, thereby allowing the identification of virulence genes, or genes required for in vivo bacterial survival. Finally, the hollow fiber technique permits evaluation of the effects of soluble factors secreted by encapsulated organisms or other cells of interest on host immune response and can be used as a vaccination strategy.
In one embodiment, the hollow fiber encapsulation/implantation technique is used to study the behavior of microorganisms in the extracellular compartment of an animal. In one embodiment, the hollow fiber encapsulation/implanatation technique is used to encapsulate bacilli in semi-diffusible hollow fibers which are implanted subcutaneously into mice, creating an in vivo model of tuberculosis. This embodiment grants a unique opportunity to study the behavior of extracellular Mycobacterium tuberculosis in animals. Specifically, this embodiment provides insight into the adaptive mechanisms employed by M. tuberculosis during persistence in the host through global gene expression analysis, and evaluation of specific gene-deficient mutants for the ability to persist. In one embodiment, this model can be used to study the activity of various drugs against dormant bacilli in vivo.
Mycobacterium tuberculosis residing within pulmonary granulomas and cavities represents an important reservoir of persistent organisms during human latent tuberculosis infection. Granulomatous lesions develop around these hollow fibers, and in this microenvironment, the organisms demonstrate an altered physiologic state characterized by stationary-state colony-forning unit counts and decreased metabolic activity. Moreover, these organisms show an antimicrobial susceptibility pattern similar to persistent bacilli in current models of tuberculosis chemotherapy in that they are more susceptible to the sterilizing drug rifampin, than to the bactericidal drug isoniazid. This model of extracellular persistence within host granulomas can be used to study both gene expression patterns and mutant survival patterns.
In one embodiment the hollow-fiber method is used to cultivate microbial species which either are impossible or difficult to cultivate in vitro or in non-hollow-fiber animal models. These microbes could be cultivated in relatively large quantities within hollow fibers implanted with an appropriate animal host for research purposes, for purposes of developing anti-microbials, for the purposes of clinical diagnosis, for the purpose of deriving pure microbial products in relatively large quantities, for the purpose of developing improved diagnostics, for the purpose of developing vaccines, or for other suitable purposes. Such microbes include but are not limited to: Mycobacterium leprae (agent of leprosy), Treponema pallidum (agent of syphilis), and hepatitis C virus with appropriate carrier eukaryotic cells, or other suitable microbes described herein.
In one embodiment, the hollow fiber technique employs semi-diffusible fibers. In one embodiment, the fibers are commercially available fibers, such as, e.g., PVDF hollow fibers with 500 kDa molecular weight cutoff available from Spectrum Laboratories, Inc., 18617 Broadwick Street, Rancho Dominguez, Calif. 90220 USA. In one embodiment, the physical parameters of the hollow fibers can be altered to optimize their utility. For example, the fibers are composed of various synthetic compounds in order to reduce immunogenicity. The internal diameter of the fibers can be increased to accommodate a great volume of cells of interest. In addition, the pore size of the fiber membranes can be varied, according to the specific cell types or secreted factors studied. In one embodiment, the hollow fibers are implanted in the subcutaneous or intraperitoneal spaces of experimental mice, rats, guinea pigs, and rabbits. In one embodiment, for the study of lung-tropic pathogens, fibers may be deposited bronchoscopically in the airways of rabbits.
The present invention is further illustrated by the following examples, which should not be construed as limiting in any way. The practice of the present invention will employ, unless otherwise indicated, conventional techniques that are within the skill of the art.
EXAMPLES
Example 1
This example demonstrates the physical containment of extracellular M. tuberculosis within mice as a means of comparing in vivo-cultivated persistent bacteria with in vitro latency models. This example demonstrates the encapsulation of tubercle bacilli in polyvinylidene fluoride (PVDF) hollow fibers (Hollingshead, M. G., et al. Life Sci 57:131-141 (1995), Xu, Z. Q., et al. Bioorg Med Chem Lett 9:133-138 (1999)) and the implantation of these fibers into the subcutaneous space of mice (FIG. 1). The PVDF fibers have a molecular weight cutoff of 500 kDa, which allows for the diffusion of small soluble molecules, but prevents the entry of host immune cells and the exit of intact bacilli. The prevention of direct host-pathogen interactions imposed by the physical properties of the hollow fibers provides a unique opportunity to study the behavior of extracellular M. tuberculosis in the host.
Materials and Methods
Strains: M. tuberculosis H37Rv expressing firefly luciferase (H37Rv-lux) was passaged twice through mice and used for all infections. The organisms were grown in plastic roller bottles at 37° C. in Middlebrook 7H9 liquid broth (Difco Laboratories) supplemented with 10% OADC (Becton Dickinson), 0.5% glycerol and 0.05% Tween-80.
Hollow fiber assay: Infection with M. tuberculosis was achieved in 6-8 week old female hairless immunocompetent SKH1 mice (Charles River Laboratories) using the hollow fiber encapsulation/ implantation technique as described previously. Hollingshead, M. G., et al. Life Sci 57:131-141 (1995), Xu, Z. Q., et al. Bioorg Med Chem Lett 9:133-138 (1999). Briefly, liquid cultures of M. tuberculosis were inoculated into the lumen of polyvinylidene fluoride (PVDF) hollow fibers (molecular weight cutoff 500 kDa, Spectrum Laboratories) with a syringe and 20-gauge needle. The ends of the hollow fibers were heat-sealed and individual fibers were prepared by heat-sealing at 2-cm intervals. Mice were anesthetized by intraperitoneal injection of Avertin (2,2,2-tribromoethanol) 240 mg/kg (Sigma-Aldrich) and the dorsal skin surface was sterilized with 70% ethanol. A small incision was made at the nape of the neck and one fiber was deposited into the subcutaneous space of each flank with a tumor trocar (2 fibers/mouse). Incisions were closed with a small surgical clip (FIG. 1). For immunology studies, Balb-C/J and IFNγ−/−(B6-IFNgtmITSIJ) were used (Jackson Labs). For experiments involving hollow fiber-encapsulated M. tuberculosis incubated in vitro, hollow fibers were incubated at 37° C. in 50-ml conical tubes containing 20 ml Middlebrook 7H9 liquid broth (Difco) supplemented with 10% OADC (Becton Dickinson), 0.5% glycerol and 0.05% Tween-80. For determination of colony-forming unit (CFU) counts, hollow fibers were recovered from mice at the time of sacrifice and their contents plated on 7H10 agar plates (Fischer Scientific). Log-transformed CFU values were used to calculate averages and standard errors for graphing purposes.
Luciferase assay: The luciferase reaction was initiated by the addition of 150 μl of luciferin (1 mM in 0.1 M Na citrate; Promega) to 50 μl of each undiluted sample. Luminescence was detected 20 seconds after the addition of substrate by a TD-20/20 luminometer (Turner Designs). Three successive measurements were made and the average relative light unit (RLU) values recorded. The log10 of average RLU values multiplied by 1000 was represented graphically.
Microarrays: Hollow fibers each containing 106 bacilli were implanted into 15-20 SKH1 mice (2 hollow fibers/mouse). Hollow fibers were retrieved from mice 10 days after hollow fiber encapsulation and fiber contents were recovered and snap-frozen. Pooled samples were suspended in TriZOL reagent (GIBCO/BRL), and M. tuberculosis membranes were disrupted using zirconia/silica beads in a bead beater. M. tuberculosis RNA was recovered by centrifugation, chloroform extraction, and isopropyl alcohol precipitation, and purified using RNeasy column (Qiagen) as previously described. Sherman, supra, Betts, J. C., et al. Mol Microbiol 43:717-731 (2002). The same steps were followed to extract and purify RNA from mid-logarithmic phase (A600, 0.600-0.850) M. tuberculosis H37Rv-lux cultures grown in plastic roller bottles at 37° C. in supplemented liquid broth. Fluorescently labeled cDNA was generated using Powerscript (Clontech), using fluorescent dyes Cy3 and Cy5. These cDNA were competitively hybridized on microarray slides containing commercially available (Operon) M. tuberculosis 70-mer oligo-nucleotides representing all opening reading frames annotated in the H37Rv genome sequencing project (Cole, S. T., et al. Nature 393:537-544 (1998)), and fluorescence intensity data were collected with a GenePix 4000 scanner (Axon Instruments) with GenePix Pro 4.0 software. Data were normalized based on total intensity of good-quality spots above background for each channel, and ratios of in vivo hollow fiber to in vitro cDNA were calculated based on normalized data. In this assay, the ratio of the signal from in vivo hollow fiber samples to that of in vitro control samples for a given open reading frame (ORF) should represent the relative abundance of the transcripts of that ORF under the two conditions. Three biological replicates were performed, and microarray samples were reverse-labeled in one experiment. Significant differential regulation of genes was defined by ≧2-fold change in gene expression as compared to control samples and p<0.01.
Quantitative real-time RT-PCR: RNA samples were treated with DNA-free kit (Ambion) according to the instructions of the manufacturer, and DNA contamination was excluded by PCR amplification using primers for Rv2031 (acr) and absence of PCR product on gel electrophoresis. Reverse transcription of RNA samples (˜0.5 μg RNA/sample) was accomplished using random hexamer primers (Invitrogen; 0.5 μg/reaction) and Powerscript Reverse Transcriptase (Clontech). Real-time PCR was performed on cDNA using the SYBR-green assay and Premix D (Epicentre) for all samples, and fluorescence was measured by iCycler (Biorad). Gene expression was compared to that of sigA, a M. tuberculosis housekeeping gene.
Transposon mutants: Random insertion mutagenesis of M. tuberculosis CDC 1551 strain was performed using the Himar1 transposon as previously described. Rubin, E. J., et al. Proc Natl Acad Sci USA 96:1645-1650 (1999), Lamichhane, G., M. et al. Proc Natl Acad Sci USA 100:7213-7218 (2003). Transposon (Tn) insertion sites were identified by sequencing the insertion junction as previously described (Lamichhane, supra). One hundred different mutants, each with Tn insertion within the proximal 80% or proximal to the distal 100 base pairs of the ORF, were randomly selected from the library of mutants. Each of the selected mutants was separately grown in 37° C. in supplemented Middlebrook 7H9 liquid broth to an A600 of 0.8-1.0, and 2 master pools, each consisting of 50 mutants, were prepared by mixing an equal volume of culture of each mutant. This mixture was diluted to an A600 of 0.1 and the latter was used to inoculate the hollow fibers. In separate experiments, the relMtb-disrupted Tn insertion mutant was grown separately in vitro using the conditions described above to an A600 of ˜1.0, and diluted 1:10. The diluted culture was then added in a 1:1 ratio to a similarly grown and diluted culture of wild-type CDC1551. The ΔrelMtb/wild-type culture suspension was then encapsulated in hollow fibers as described above and either implanted subcutaneously into mice or incubated in vitro. Recovered hollow fiber samples were plated on Middlebrook 7H10 plates (Difco) and on 7H10 plates containing kanamycin 20 μg/ml (the transposon insertion contains a kanamycin resistance gene. Lamichhane, supra).
Viability assay: The LIVE/DEAD BacLight Bacterial Viability Kit (Molecular Probes) was used to assay for mycobacterial viability after removal from hollow fibers. Briefly, Component A (SYTO 9 green-fluorescent nucleic acid stain, 3.34 mM) and Component B (propidium iodide, 20 mM) were mixed in equal volumes. An equivalent of 3 μ1 of this 1:1 nucleic acid stain mixture was added to each 1 ml of sample, and samples were incubated in the dark for ≧15 min. As a control, in vitro-grown M. tuberculosis H37Rv-lux was treated for 3 hours with 70% ethanol in order to promote mycobacterial death and highlight differences in fluorescence staining between live and dead bacilli (FIG. 3a). Samples were treated with 20 μl of 4% paraformaldehyde (which does not alter cell permeability characteristics) for ≧15 min and samples were observed using epifluorescence microscopy (Nikon Eclipse E800). Images were obtained using a Nikon Digital Camera DXM1200 and processed using Spot Version 3.4 software.
Antibiotic studies: Mice received a powdered diet containing 1% sugar with either 0.02% (by weight) rifampin (Sigma) or 0.05% isoniazid (Sigma), beginning 14 days after implantation of hollow fibers. Untreated control mice received powdered diet containing 1% powdered sugar alone. In separate experiments, mice received diet containing 2.5% sugar alone (untreated controls) or with 0.25% moxifloxacin beginning on day 1 after hollow fiber implantation. Mouse dietary consumption was measured and recorded for all groups on a daily basis. At the time of sacrifice, blood was obtained from antibiotic-treated mice by cardiac puncture, mouse serum was separated, and serum samples were stored at −70° C. until the time of analysis. Mouse serum samples were evaluated for determination of serum antibiotic concentrations.
A complete list of all transposon (Tn) mutants used, including information on the gene mutated and the exact coordinate of Tn insertion, is presented in Table 2. DosR genes which fulfilled one but not both criteria for significant upregulation are also presented in Table 2. The complete lists of genes found to be significantly upregulated or downregulated by microarray analysis in hollow fiber-encapsulated bacilli in vivo, as well as all genes not found to be differentially regulated, are also presented in Table 2.
Results
Inhibition of Growth of Encapsulated Tubercle Bacilli In Vivo (FIG. 2)
M. tuberculosis H37Rv expressing firefly luciferase (H37Rv-lux) was encapsulated in hollow fibers and implanted into the subcutaneous space of mice or incubated at 37° in supplemented Middlebrook 7H9 broth. Whereas hollow fiber-encapsulated bacilli incubated in vitro multiply exponentially for 14-21 days before reaching a plateau, bacilli encapsulated in hollow fibers and implanted into mice rapidly achieve stationary-state colony-forming unit (CFU) counts (FIG. 2). The continued growth of in vitro hollow fiber-encapsulated bacilli implies that the failure of in vivo hollow fiber-encapsulated bacilli to multiply is not simply a result of physical containment imposed by the internal dimensions of the hollow fibers.
Assessment of Bacillary Viability and Metabolic Activity
In order to further investigate the CFU equilibrium in hollow fiber-encapsulated bacilli in vivo, the bacilli recovered from fibers was examined using a bacterial viability assay in which live M. tuberculosis bacilli emit green fluorescence, while dead bacilli fluoresce red, based on differences in cell membrane permeability to the two nucleic acid stains used in the assay (FIG. 3). If the stationary-state CFU counts observed in hollow fiber-encapsulated bacilli in vivo represented an equilibrium between bacillary multiplication and death, one would expect that live-staining bacilli would comprise a very small proportion of all staining bacilli, due to the vast accumulation of dead bacilli over time. However, approximately half of all staining organisms on days 21 (FIG. 3b) and 28 (FIG. 3c) after hollow fiber implantation were determined to be alive by their staining properties, consistent with the hypothesis that these organisms are in a slowly replicating or non-replicating persistent state. In addition, a significant lag time in the appearance of colonies after plating the in vivo-cultivated bacilli was detected, as compared to hollow fiber-encapsulated bacilli incubated in vitro. At the day 21 time point, microcolonies were detected 10-11 days after plating in the in vitro hollow fiber samples, whereas these were not detectable in the in vivo hollow fiber samples until 14 days after plating. Furthermore, average colony size for in vivo hollow fiber samples was significantly smaller than corresponding in vitro hollow fiber samples when examined 17 days after plating (FIG. 9). These data are consistent with the hypothesis that hollow fiber-encapsulated M. tuberculosis implanted in mice rapidly enters a state of decreased replication.
Because the oxidation of luciferin to oxyluciferin and photons (which can be detected by a luminometer at ˜560 nm) is an ATP-dependent process, the luciferase assay may be used as an indirect measure of the ATP content of cells. In log-phase in vitro cultures of M. tuberculosis (i.e., not encapsulated in fibers), the relationship between CFU and relative light units (RLU) is linear (FIG. 4a). Hollow fiber-encapsulated M. tuberculosis grown in vitro maintains baseline metabolic activity, as reflected by RLU values that closely parallel CFU counts at the corresponding time points (FIG. 4b). On the contrary, RLU values of in vivo-cultivated hollow fiber-encapsulated bacilli on days 14 and 21 after implantation (FIG. 4b) were 0.8 log10 and 0.93 log10, respectively, less than predicted based on the corresponding CFU values (FIG. 2) and the proportionality of CFU:RLU (FIG. 4a), suggesting reduced metabolic activity of these organisms, as measured by the luciferase assay. These data suggest that in vivo hollow fiber-encapsulated M. tuberculosis bacilli rapidly enter a state of decreased metabolic activity.
Antibiotic Susceptibility of Hollow Fiber-encapsulated Bacilli
Animal and human chemotherapy studies have pointed to several populations of M. tuberculosis in the mammalian host, including rapidly-multiplying, and non-replicating or sporadically-replicating persistent bacilli. Mitchison, D. A. J Antimicrob Chemother 29:477-493 (1992), McKinney, J. D. Nat Med 6:1330-1333 (2000). In keeping with this mixed bacterial population model, drug treatment studies have demonstrated that the sterilizing drug rifampin is more active against sporadically-multiplying and persistent bacilli than the bactericidal drug isoniazid, the former drug requiring a significantly shorter duration of therapy in order to achieve acceptable relapse rates. Lecoeur, H. F., et al. Am Rev Respir Dis 140:1189-1193 (1989); Am Rev Respir Dis 145:36-41 (1992); Dickinson, J. M., and D. A. Mitchison. Am Rev Respir Dis 123:367-371 (1981); Jasmer, R. M., et al. N Engl J Med 347:1860-1866 (2002); Fox, W., and D. A. Mitchison. Am Rev Respir Dis 111:325-353 (1975). Thus, rifampin likewise should be more effective than isoniazid against hollow fiber-encapsulated M. tuberculosis implanted into mice if these bacilli were truly in a slowly-replicating, metabolically quiescent state. In order to allow bacilli to enter this altered physiologic state, antibiotics were not initiated until 14 days after hollow fiber implantation. Experimental groups of mice received either powdered diet containing isoniazid 0.05% or rifampin 0.02% (by weight), while untreated mice received antibiotic-free diet. Serum concentrations of each antibiotic were measured in sacrificed mice at each timepoint, and were found to be at least 10 times greater than their respective MIC90 (Table 1).
TABLE 1
|
|
Serum isoniazid and rifampin concentrations in mice
|
Isoniazid
SKH1 mice (0.02% diet):Humans (5 mg/kg):
Cmean (mg/L)AUC (mg h/L)Cmax (mg/L)AUC (mg h/L)
|
0.77 ± 0.2118.5 ± 5.05.4 ± 2.0a19.9 ± 6.1a
7.1 ± 1.9b48.2 ± 1.5b
|
Rifampin
SKH1 mice (0.05% diet):Humans (10 mg/kg):
Cmean (mg/L)AUC (mg h/L)Cmax (mg/L)AUC (mg h/L)
|
3.09 ± 0.4174.2 ± 9.814.91117.93
|
a= rapid acetylators
|
b= slow acetylators
|
Daily area under the serum concentration-time curve (AUC) properties of each dietary regimen in mice were found to be similar to the AUC in humans after standard daily doses of each drug. Kim, Y. G., Clin Pharmacol Ther 54:612-620 (1993), Kenny, M. T., and B. Strates. Drug Metab Rev 12:159-218 (1981). Although isoniazid was somewhat effective against bacilli in the hollow fiber model in vivo, the activity of rifampin was more consistent and the magnitude of its effect greater than that of isoniazid at each time point, resulting in a 3-log kill by 21 days after initiation of therapy (FIG. 5). When mice were treated with the new 8-methoxyfluoroquinolone moxifloxacin, which has potent bactericidal activity against M. tuberculosis in mice (Nuermberger, E. L., et al. Am J Respir Crit Care Med. (2003)), beginning on day 1 after hollow fiber implantation, fibers were culture-negative by day 14 after daily therapy (FIG. 10). These data suggest that by 14 days after hollow fiber implantation, hollow fiber-encapsulated M. tuberculosis has entered an altered physiologic state in which bacilli are more resistant to the activity of anti-TB drugs, but more sensitive to rifampin than to isoniazid, consistent with the antibiotic susceptibility of persistent bacilli in human latent TB infection. Lecoeur, supra; Am Rev Respir Dis 145:36-41 (1992); Dickinson, J. M., supra; Jasmer, R. M., supra; Fox, W., supra.
Granuloma Formation Surrounding Bacilli-containing Hollow Fibers in Mice
Interestingly, the progressive formation of thick, granuloma-like lesions encasing hollow fibers containing M. tuberculosis H37Rv-lux was observed (FIG. 6d-f), but such formation was not observed in those containing liquid broth alone (FIG. 6a-c). By day 28 after hollow fiber implantation, histologic analysis revealed much greater cellular infiltration of macrophages, lymphocytes, and fibroblasts in the tissues surrounding hollow fibers continuing tubercle bacilli (FIG. 6j-k), as compared to those containing liquid broth (FIG. 6g-i). Acid-fast staining of the surrounding tissue revealed no detectable bacilli, and inflammatory cells were not detected by microscopy within the hollow fibers. These results suggest the possibility that M. tuberculosis secretes soluble factors that diffuse from the fibers, leading to recruitment of host inflammatory cells. The formation of granulomatous lesions encasing hollow fibers may create a hostile microenvironment in which bacilli are forced to reduce their replication and metabolic activity, consequently becoming more susceptible to rifampin than to isoniazid.
Containment of Intra-fiber Bacillary Growth In Vivo is Immune-mediated and IFN-γ-dependent
In addition to local granuloma formation, significant spleen enlargement was detected as early as 14 days after hollow fiber implantation in mice which had implanted fibers containing M. tuberculosis as compared to those which had implanted fibers containing media (FIG. 7a), suggesting a systemic immune response in the former group of mice. In order to further investigate the phenomena of granuloma formation and splenomegaly and to exclude the possibility that intra-fiber bacillary growth containment in vivo is mediated exclusively by nutrient starvation, the growth of hollow fiber-encapsulated M. tuberculosis in interferon gamma (IFNγ)-deficient mice was studied. Briefly, approximately 500 bacilli were encapsulated in each hollow fiber, and fibers were implanted subcutaneously either into wild type Balb-C/J mice or Balb-C/J IFNγ-deficient mice, and colony counts were measured 28 days after implantation. Interestingly, although there was some growth of bacilli in wild type mice over 28 days, colony-forming unit counts of hollow fiber-encapsulated M. tuberculosis in IFNγ-deficient mice were 1.3 log10 higher at 28 days than those in wild type mice, suggesting that IFNγ plays a role in containment of intra-fiber bacillary growth in vivo (FIG. 7b).
Reduced Survival of relMtb-deficient M. tuberculosis in Hollow Fibers Implanted into Mice
As proof-of-principle to determine whether this model could predict genes essential for extracellular persistence within granulomas, a library of mutants was generated by transposon mutagenesis of M. tuberculosis CDC 1551 strain using the Himar1 transposon as previously described. Lamichhane, supra. One hundred genetically-defined mutants, each deficient in a specific gene (as defined by transposon insertion within the proximal 80% or proximal to the distal 100 base pairs of the open reading frame of each particular gene (Id)), were selected from the library and divided into 2 master pools, each consisting of 50 mutants (Table 2).
TABLE 2
|
|
Transposon Insertion Mutant Pool 1
MT No.Rv No.Open reading frame (ORF) descriptionORF sizePOI
|
18501801PPE family protein (PPE)1271210
15151469cation transporting ATPase, E1-E2 family ctpD1973838
524503cyclopropane-fatty-acyl-phospholipid synthase 2965746
(cmaA2)
20361983PE_PGRS167660
26602583GTP pyrophosphokinase (relMtb)2381322
20051956DNA binding protein, putative449319
15511502HP899632
39363828IS1537, resolvase611418
30682990HP86062
154146conserved HP941126
33953296ATP-dependent helicase, putative (1 hr)4541631
115106conserved HP119617
364349HP54276
33993300RNA pseudouridylate synthase family91730
30112941acyl-CoA synthase, FadD28174274
19871937Ferredoxin reductase, electron transfer2519459
38483740HP13461144
344329MitM-related protein626462
34723364Conserved HP392253
27942721Conserved HP2099279
35033395HP641169
24142349Phospholipase C (plcC)1526476
27872714Conserved HP974240
315302transcriptional regulator, TetR family632336
31533068Phosphoglucomutase (pgmA)164376
262248FAD flavoprotein oxidase1940859
267254Cobinamide/cobinamide phosphate52485
guanylyltransferase (cobU)
19621911Phosphatidylethanolamine binding protein (lppC)61765
953926HP1076829
917893Conserved HP97756
17801738HP (b/w Rv1737/1738)461129
738711arylsulfatase (atsA)22711895
183174virulence factor mce protein1547321
34333330penicillin binding protein1217682
16281592Conserved HP13671072
7771Conserved HP716310
35803476sugar transporter family protein (kgtP)1349103
35693463HP857624
34973390lipoprotein, putative (lpqD)710399
28292759Conservative HP395111
4035AMP-binding family protein (fadD34)1688103
24102345Conserved HP1982282
38063703Conservative HP12771116
15941542protozoan/cianobacterial globin family protein410125
(glbN)
204194ABC transporter, ATP-binding protein (HP)37251738
35103402Degt/DnrJ/EryC1 family protein (HP)123877
35833479HP32271514
10991069HP18111063
25452469HNH endonuclease family protein66856
23152254HP455226
|
Transposon Insertion Mutant Pool 2
MT No.Rv No.ORF descriptionORF sizePOI
|
24832410Conserved HP977598
35573451serine exterase, cutinase family743586
38383733HP500350
19791927Conserved HP774218
15011454Quinone oxidoreductase, putative (qor)1007172
1533.21488SPFH domain/Band 7 family protein1145877
14021358transcriptional regulator, LuxR family3479896
27852712HP1058727
29562888amidase family protein (amiC)14211123
16711635HP16971108
676648glycosyl hydrolase4196794
11091078proline-rich antigen (pra)722312
618589virulence factor (mce2)1214867
23292267HP1166531
34583350PPE113557007
18611813HP431153
19561905D-amino acid oxidase96264
17651724HP419300
27492675Conserved HP752564
20862027GAF family protein1721273
2801.12730HP476239
16081557membrane protein, mmpL family (mmpL6)1193921
871848cysteine synthase, putative (cysM3)1213415
652624Conserved HP395112
29822914Ser/Thr protein kinase (pknI)181143
11721139Conserved HP374235
28312761Type I restriction system specificity protein109466
4842Transcriptional regulator, MarR family626427
7973ABC transporter, ATP binding protein99236
27802707Conserved HP974748
1172.11139HP (b/w Rv1139/1140)218154
787762HP545123
11841147IS Mt 1 transposase (HP)927387
15561508HP1799488
22802222glutamine synthase (glnA2)13401093
202192Conserved HP1100787
16251590Conserved HP23950
30052935polyketide synthase (ppsE)4464566
25632488Transcriptional regulator, LuxR family (HP)3341919
28832816Conserved HP34167
24072342HP293123
22392184Conserved HP1142894
34383334transcriptional regulator, MerR family438339
33373239Drug transporter31972589
10596PPE1391715
31763091HP1691187
18341785P450 heme-thiolate protein1280400
18681820TPP requiring enzyme ilvG (acetoactate164363
synthase)
26742599HP431333
12231186HP1616586
|
Other Up-regulated dosR Regulon Genes in the Hollow Fiber Model In Vivo
Rv No.GeneProtein FunctionFold upregulationP-value
|
79HP2.10.01
·81Transcriptional regulator1.50.003
573cCHP1.90.007
574cCHP1.80.002
2003cCHP1.70.005
2028cCHP-USPA motif1.50.006
2032acgCHP5.50.01
2624cCHP-USPA motif2.30.04
2625cCHP1.80.01
2630HP2.30.02
3127CHP9.50.02
3129CHP2.10.04
|
Rv No.GeneFoldP value
|
Complete List of Significantly Up-regulated M. tuberculosis Genes in the Hollow
Fiber Model In Vivo
Rv0003recF3.30.006
Rv0008cHP2.30.002
Rv0028HP2.20.005
Rv0035fadD342.30.009
Rv0064HP4.40.001
Rv0080HP2.90.0009
Rv0099fadD103.00.003
Rv0101Nrp3.30.008
Rv0102HP4.70.004
Rv0105cHP2.40.0003
Rv0116cHP2.60.004
Rv0131cfadE12.40.009
Rv0136HP2.00.008
Rv0188HP2.70.0002
Rv0197HP3.10.006
Rv0204cHP2.80.002
Rv0211pckA2.61.90E−06
Rv0216HP2.20.005
Rv0251cHsp3.90.004
Rv0255ccobQ2.29.40E−08
Rv0260cHP2.20.006
Rv0261cnarK32.20.007
Rv0282HP2.20.001
Rv0284HP2.00.0008
Rv0287HP2.20.0004
Rv0288HP2.90.004
Rv0309HP2.10.004
Rv0324HP2.40.006
Rv0336HP2.40.009
Rv0355cPPE11.60.008
Rv0367cHP2.10.002
Rv0387cHP8.10.003
Rv0388cPPE3.50.006
Rv0399clpqK2.60.006
Rv0406cHP2.20.008
Rv0412cHP2.52.70E−05
Rv0419lpqM3.30.004
Rv0440groEL23.60.0005
Rv0441cHP2.90.0009
Rv0465cHP3.60.005
Rv0483HP2.10.005
Rv0489gpm2.40.0003
Rv0515HP3.80.006
Rv0537cHP2.60.002
Rv0538HP2.70.002
Rv0549cHP2.10.001
Rv0569HP4.90.007
Rv0572cHP2.20.002
Rv0588HP2.30.003
Rv0592HP4.80.002
Rv0623HP2.50.006
Rv0648HP3.10.008
Rv0687HP2.40.004
Rv0727cfucA2.60.008
Rv0772purD2.60.003
Rv0782ptrBa2.00.009
Rv0790cHP4.00.008
Rv0791cHP2.30.002
Rv0794clpdB2.30.008
Rv0828cHP3.70.003
Rv0836cHP6.70.004
Rv0846cHP2.30.007
Rv0873fadE102.40.007
Rv0879cHP2.20.003
Rv0885HP3.84.80E−06
Rv0887cHP2.10.007
Rv0892HP2.90.002
Rv0897cHP2.10.001
Rv0923cHP2.40.004
Rv0925cHP3.00.007
Rv0942HP3.30.007
Rv0962cHP2.10.0001
Rv0966cHP4.60.002
Rv0967HP2.50.006
Rv0970HP22.70.009
Rv0978cPE_PGRS5.10.0005
Rv0980cPE_PGRS3.30.009
Rv0982HP6.40.008
Rv0988HP7.00.004
Rv1018cglmU8.00.002
Rv1029kdpA4.60.0009
Rv1031kdpC2.60.005
Rv1032cHP3.00.002
Rv1039cPPE3.20.004
Rv1073HP2.69.70E−07
Rv1075cHP3.00.009
Rv1128cHP3.40.003
Rv1131gltA14.50.004
Rv1139cHP3.90.003
Rv1169cPE2.80.004
Rv1204cHP4.20.005
Rv1277HP2.10.007
Rv1302rfe2.51.50E−07
Rv1367cHP2.40.002
Rv1375HP2.30.0003
Rv1393cHP3.50.006
Rv1394cHP2.40.0006
Rv1405cHP2.90.0009
Rv1406fmt3.00.004
Rv1493mutB2.40.001
Rv1499HP2.70.003
Rv1513HP2.00.009
Rv1517HP2.40.004
Rv1538cansA3.30.003
Rv1556HP2.40.007
Rv1572cHP2.90.009
Rv1592cHP2.40.0007
Rv1638uvrA2.22.10E−08
Rv1702cHP2.70.002
Rv1730cHP3.50.003
Rv1733cHP3.00.004
Rv1752HP5.70.004
Rv1753cPPE7.30.005
Rv1759cPE_PGRS3.00.006
Rv1782HP2.30.0002
Rv1801PPE2.80.005
Rv1808PPE2.80.003
Rv1809PPE2.40.001
Rv1825HP3.80.002
Rv1907cHP5.60.004
Rv1909cfurA2.80.004
Rv1930cHP2.10.0008
Rv1936HP3.20.006
Rv1954cHP2.90.007
Rv1956HP2.30.0009
Rv1970lprM3.10.006
Rv1972HP3.10.001
Rv1981cnrdF3.50.006
Rv1984cHP3.70.009
Rv1986HP3.80.003
Rv1988HP2.90.005
Rv1990cHP2.70.0001
Rv1992cctpG2.31.70E−07
Rv1995HP7.10.009
Rv1997ctpF13.20.007
Rv2005cHP2.20.004
Rv2015cHP2.28.30E−06
Rv2018HP2.31.30E−06
Rv2026cHP3.50.002
Rv2030cHP7.83.30E−05
Rv2031chspX25.00.009
Rv2055crpsR22.60.003
Rv2056crpsN24.39.90E−07
Rv2057crpmG5.90.001
Rv2059HP2.60.0003
Rv2068cblaC2.00.001
Rv2069sigC2.20.004
Rv2072ccobL2.10.004
Rv2089cpepE4.40.005
Rv2113HP2.10.003
Rv2114HP2.10.003
Rv2122chisI3.40.003
Rv2131ccysQ2.40.006
Rv2166cHP3.00.003
Rv2170HP3.90.003
Rv2173idsA23.90.003
Rv2225panB2.12.80E−05
Rv2227HP2.50.004
Rv2269cHP3.10.003
Rv2273HP5.10.009
Rv2306cHP3.30.002
Rv2322crocD12.10.009
Rv2330clppP0.00.004
Rv2343cdnaG2.70.0007
Rv2370cHP4.00.005
Rv2378cmbtG2.10.001
Rv2380cmbtE4.00.007
Rv2411cHP2.50.002
Rv2429ahpD3.50.002
Rv2437HP2.20.001
Rv2455cHP2.41.60E−10
Rv2497cpdhA2.00.0008
Rv2512cHP2.30.0005
Rv2524cFas4.50.007
Rv2531cAdi5.30.005
Rv2566HP2.40.003
Rv2577HP3.40.008
Rv2601speE2.40.001
Rv2617cHP3.10.0007
Rv2621cHP2.30.009
Rv2623HP25.80.0002
Rv2626cHP14.60.0001
Rv2627cHP8.00.008
Rv2628HP8.40.004
Rv2631HP2.10.001
Rv2643arsC3.50.001
Rv2657cHP2.80.008
Rv2659cHP3.30.003
Rv2660cHP3.30.006
Rv2700HP2.20.009
Rv2706cHP2.20.001
Rv2707HP3.10.003
Rv2710sigB4.77.40E−06
Rv2712cHP2.40.006
Rv2713HP2.70.008
Rv2725chflX2.00.007
Rv2744c35kd_ag3.30.0001
Rv2757cHP3.10.009
Rv2780Ald2.40.001
Rv2787HP2.20.008
Rv2833cugpB3.80.007
Rv2835cugpA2.40.003
Rv2843HP2.20.004
Rv2863HP2.00.008
Rv2887HP2.80.009
Rv2932ppsB2.30.008
Rv2963HP3.50.008
Rv2979cHP2.80.001
Rv3021cPPE2.40.003
Rv3047cHP2.00.004
Rv3048cnrdG2.90.007
Rv3074HP2.90.006
Rv3076HP4.00.003
Rv3080cpknK2.50.0002
Rv3082cvirS3.40.004
Rv3088HP2.10.0009
Rv3105cprfB2.90.001
Rv3126cHP2.20.0001
Rv3128cHP3.20.004
Rv3130cHP15.40.0004
Rv3131HP11.40.0008
Rv3132cHP4.80.001
Rv3133cHP2.10.007
Rv3134cHP7.10.007
Rv3139fadE245.80.004
Rv3189HP2.20.004
Rv3223csigH3.40.004
Rv3270ctpC2.40.0008
Rv3287crsbW4.60.0005
Rv3290clat5.60.009
Rv3334HP3.30.0002
Rv3335cHP3.00.009
Rv3340metC2.80.0007
Rv3343cPPE3.40.005
Rv3468crmlB33.20.003
Rv3515cfadD193.10.009
Rv3521HP2.90.009
Rv3531cHP2.40.003
Rv3538ufaA22.10.003
Rv3539PE7.60.006
Rv3650PE4.60.008
Rv3651HP3.20.004
Rv3660cHP2.00.0004
Rv3669HP3.00.004
Rv3698HP2.60.008
Rv3811csp2.00.005
Rv3839HP2.60.002
Rv3848HP2.90.002
Rv3862cHP5.30.0003
Rv3894cHP5.20.002
Rv3896cHP2.20.007
Rv3898cHP2.20.0007
Rv3900cHP2.00.003
Rv3912HP2.70.003
Significantly Down-regulated M. Tuberculosis Genes in the Hollow Fiber
Model In Vivo
Rv0172HP−2.5 2.0E−04
Rv0682rpsL−2.2 6.6E−05
Rv0683rpsG−2.5 1.2E−07
Rv0692HP−5.4 1.5E−09
Rv0693pqqE−2.2 4.3E−05
Rv0694lldD1−2.1 4.2E−05
Rv0696HP−2.3 5.0E−04
Rv0700rpsJ−2.8 4.5E−06
Rv0701rplC−2.2 1.5E−05
Rv0703rplW−2.4 1.7E−07
Rv0704rplB−2.6 2.0E−04
Rv0706rplV−2.3 3.5E−05
Rv0708rplP−2.4 4.0E−04
Rv0710rpsQ−2.1 9.0E−04
Rv0716rplE−2.4 1.3E−10
Rv0722rpmD−2.2 3.5E−05
Rv1037cHP−2.5 3.9E−11
Rv1038cHP−2.5 1.4E−08
Rv1196PPE−2.6 1.5E−06
Rv1197HP−2.1 3.9E−08
Rv1198HP−2.4 2.1E−06
Rv1304atpB−3.3 4.8E−07
Rv1306atpF−3.3 9.5E−10
Rv1308atpA−2.2 6.6E−05
Rv1310atpD−3.8 1.1E−10
Rv1311atpC−2.7 6.5E−07
Rv1312HP−2.1 2.7E−06
Rv1448ctal−2.9 1.1E−06
Rv1871cHP−2.2 2.0E−04
Rv1872clldD2−2.5 5.4E−08
Rv1886cfbpB−2.3 5.2E−05
Rv2347cHP−2.0 6.8E−07
Rv2391nirA−2.9 4.0E−06
Rv2392cysH−2.2 2.0E−03
Rv2393HP−2.1 5.0E−03
Rv2986chupB−2.3 5.0E−07
Rv3148nuoD−2.2 3.2E−05
Rv3308pmmB−2.2 3.1E−08
Rv3456crplQ−2.3 3.0E−10
Rv3477PE−4.1 5.2E−09
Rv3478PPE−2.9 2.3E−11
Rv3584lpqE−2.4 2.0E−06
Rv3619cHP−2.1 7.9E−08
Rv3620cHP−3.3 7.1E−07
Rv3648ccspA−7.7 1.5E−09
Rv3774echA21−2.2 6.2E−06
Rv3804cfbpA−3.5 9.8E−08
Rv3841bfrB−2.4 9.9E−10
Rv3874HP−2.5 1.6E−05
Rv3922cHP−2.0 3.9E−05
Complete Gene Expression Profile of M. tuberculosis in the Hollow Fiber
Model In Vivo
Rv0001dnaA7.620.01408
Rv0002dnaN1.510.04068
Rv0003recF3.260.00577
Rv0004hypothetical protein Rv00041.980.00205
Rv0005gyrB0.740.00000
Rv0006gyrA2.340.05837
Rv0007hypothetical protein Rv00071.050.38482
Rv0008chypothetical protein Rv0008c2.260.00151
Rv0009ppiA0.870.22407
Rv0010chypothetical protein Rv0010c1.590.00510
Rv0011chypothetical protein Rv0011c1.560.07362
Rv0012hypothetical protein Rv00121.310.00139
Rv0013pabA1.440.12174
Rv0014cpknB1.320.11038
Rv0015cpknA0.740.02424
Rv0016cpbpA1.740.00266
Rv0017crodA0.660.00142
Rv0018cppp0.870.07012
Rv0019chypothetical protein Rv0019c0.800.00889
Rv0020chypothetical protein Rv0020c1.110.22439
Rv0021chypothetical protein Rv0021c1.430.06305
Rv0022chypothetical protein Rv0022c1.690.00366
Rv0023hypothetical protein Rv00232.490.03514
Rv0024hypothetical protein Rv00242.170.02775
Rv0025hypothetical protein Rv00251.200.03772
Rv0026hypothetical protein Rv00262.090.01115
Rv0027hypothetical protein Rv00271.710.00524
Rv0028hypothetical protein Rv00282.220.00536
Rv0029hypothetical protein Rv00290.860.05482
Rv0030hypothetical protein Rv00301.100.20629
Rv0031hypothetical protein Rv00311.430.00627
Rv0032bioF21.100.26952
Rv0033hypothetical protein Rv00331.580.03617
Rv0034hypothetical protein Rv00341.070.26452
Rv0035fadD342.270.00921
Rv0036chypothetical protein Rv0036c0.790.00146
Rv0037chypothetical protein Rv0037c1.690.01294
Rv0038hypothetical protein Rv00380.770.00599
Rv0039chypothetical protein Rv0039c1.380.16834
Rv0040chypothetical protein Rv0040c1.160.33581
Rv0041leuS3.940.03657
Rv0042chypothetical protein Rv0042c1.010.49169
Rv0043chypothetical protein Rv0043c1.190.09607
Rv0044chypothetical protein Rv0044c1.090.21109
Rv0045chypothetical protein Rv0045c1.960.04308
Rv0046chypothetical protein Rv0046c0.920.38568
Rv0047chypothetical protein Rv0047c0.840.17734
Rv0048chypothetical protein Rv0048c1.320.10253
Rv0049hypothetical protein Rv00491.000.49535
Rv0050ponA0.930.12565
Rv0051hypothetical protein Rv00511.580.06821
Rv0052hypothetical protein Rv00521.530.00015
Rv0053rpsF0.670.00010
Rv0054ssb1.080.13777
Rv0055rpsR0.840.00071
Rv0056rplI1.110.00179
Rv0057hypothetical protein Rv00570.760.00029
Rv0058dnaB1.460.01688
Rv0059hypothetical protein Rv00590.660.00027
Rv0060hypothetical protein Rv00601.510.00114
Rv0061hypothetical protein Rv00611.400.09329
Rv0062celA1.220.15083
Rv0063hypothetical protein Rv00631.690.05013
Rv0064hypothetical protein Rv00644.380.00147
Rv0065hypothetical protein Rv00652.720.05184
Rv0066cicd20.780.00001
Rv0067chypothetical protein Rv0067c2.600.10434
Rv0068hypothetical protein Rv00681.230.13320
Rv0069csdaA1.150.29172
Rv0070cglyA21.820.00943
Rv0071hypothetical protein Rv00711.410.00732
Rv0072hypothetical protein Rv00720.880.22075
Rv0073hypothetical protein Rv00731.110.20733
Rv0074hypothetical protein Rv00741.210.09829
Rv0075hypothetical protein Rv00751.640.04670
Rv0076chypothetical protein Rv0076c1.450.05273
Rv0077chypothetical protein Rv0077c1.550.01544
Rv0078hypothetical protein Rv00781.040.35604
Rv0079hypothetical protein Rv00792.090.01241
Rv0080hypothetical protein Rv00802.920.00092
Rv0081hypothetical protein Rv00811.520.00302
Rv0082hypothetical protein Rv00820.980.44245
Rv0083hypothetical protein Rv00831.000.49823
Rv0084hycD1.340.00281
Rv0085hycP1.060.13935
Rv0086hycQ1.030.41002
Rv0087hycE1.370.04652
Rv0088hypothetical protein Rv00881.170.11596
Rv0089hypothetical protein Rv00892.370.02677
Rv0090hypothetical protein Rv00901.560.00374
Rv0091hypothetical protein Rv00912.300.01655
Rv0092ctpA1.550.14462
Rv0093chypothetical protein Rv0093c1.060.21870
Rv0094chypothetical protein Rv0094c1.290.19103
Rv0095chypothetical protein Rv0095c1.870.01271
Rv0096PPE0.970.35955
Rv0097hypothetical protein Rv00971.130.03765
Rv0098hypothetical protein Rv00981.810.00000
Rv0099fadD102.990.00304
Rv0100hypothetical protein Rv01001.530.00000
Rv0101nrp3.300.00776
Rv0102hypothetical protein Rv01024.740.00406
Rv0103cctpB1.480.00639
Rv0104hypothetical protein Rv01044.610.01093
Rv0105crpmB2.370.00038
Rv0106hypothetical protein Rv010611.760.01758
Rv0107cctpI1.930.00515
Rv0108chypothetical protein Rv0108c0.540.00003
Rv0109PE_PGRS1.650.06724
Rv0110hypothetical protein Rv01100.850.03892
Rv0111hypothetical protein Rv01111.010.46257
Rv0112gca0.760.00839
Rv0113gmhA0.760.01189
Rv0114hypothetical protein Rv01140.700.00071
Rv0115hypothetical protein Rv01150.610.00101
Rv0116chypothetical protein Rv0116c2.560.00355
Rv0117oxyS2.910.02590
Rv0118coxcA1.010.46822
Rv0119fadD72.950.02603
Rv0120cfusA20.720.00489
Rv0121chypothetical protein Rv0121c1.510.12846
Rv0122hypothetical protein Rv01220.910.20153
Rv0123hypothetical protein Rv01231.190.14990
Rv0124PE_PGRS0.890.23289
Rv0125pepA1.100.23952
Rv0126hypothetical protein Rv01262.540.04694
Rv0127hypothetical protein Rv01271.250.01959
Rv0128hypothetical protein Rv01280.730.00247
Rv0129cfbpC21.770.04255
Rv0130hypothetical protein Rv01300.680.15876
Rv0131cfadE12.380.00940
Rv0132chypothetical protein Rv0132c1.350.39242
Rv0133hypothetical protein Rv01331.790.14979
Rv0134ephF0.980.36570
Rv0135chypothetical protein Rv0135c1.110.09391
Rv0136hypothetical protein Rv01362.030.00761
Rv0137chypothetical protein Rv0137c3.080.02914
Rv0138hypothetical protein Rv01381.860.06308
Rv0139hypothetical protein Rv01391.170.31042
Rv0140hypothetical protein Rv01401.200.17407
Rv0141chypothetical protein Rv0141c0.660.00003
Rv0142hypothetical protein Rv01420.960.22149
Rv0143chypothetical protein Rv0143c0.900.09759
Rv0144hypothetical protein Rv01440.550.00001
Rv0145hypothetical protein Rv01451.740.03616
Rv0146hypothetical protein Rv01461.400.01320
Rv0147hypothetical protein Rv01471.400.00144
Rv0148hypothetical protein Rv01481.290.02580
Rv0149hypothetical protein Rv01491.490.02632
Rv0150chypothetical protein Rv0150c1.730.01893
Rv0151cPE2.650.06380
Rv0152cPE3.890.01010
Rv0153chypothetical protein Rv0153c1.800.00454
Rv0154cfadE21.140.09008
Rv0155pntAA1.690.00132
Rv0156pntAB4.660.02338
Rv0157pntB2.070.03314
Rv0158hypothetical protein Rv01580.800.00007
Rv0159cPE1.330.04383
Rv0161hypothetical protein Rv01611.410.12934
Rv0162cadhE0.830.06726
Rv0163hypothetical protein Rv01630.960.36198
Rv0164hypothetical protein Rv01640.810.07576
Rv0165chypothetical protein Rv0165c2.560.01736
Rv0166fadD50.640.00238
Rv0167hypothetical protein Rv01670.770.02507
Rv0168hypothetical protein Rv01680.900.08838
Rv0169mce10.760.03396
Rv0170hypothetical protein Rv01700.640.02296
Rv0171hypothetical protein Rv017121.170.16935
Rv0172hypothetical protein Rv01720.400.00018
Rv0173lprK1.140.09858
Rv0174hypothetical protein Rv017433.360.16958
Rv0175hypothetical protein Rv01750.790.00423
Rv0176hypothetical protein Rv01760.660.00173
Rv0177hypothetical protein Rv01771.340.19182
Rv0178hypothetical protein Rv01781.160.33622
Rv0179clprO1.870.06484
Rv0180chypothetical protein Rv0180c3.410.06454
Rv0181chypothetical protein Rv0181c1.220.10798
Rv0182csigG0.670.00000
Rv0183hypothetical protein Rv01830.890.05013
Rv0184hypothetical protein Rv01842.000.07437
Rv0185hypothetical protein Rv01851.730.01289
Rv0186bglS1.070.23834
Rv0187hypothetical protein Rv01870.780.00100
Rv0188hypothetical protein Rv01882.650.00023
Rv0189cilvD0.900.02650
Rv0190hypothetical protein Rv01901.020.46542
Rv0191hypothetical protein Rv01911.040.20226
Rv0192hypothetical protein Rv01921.210.20679
Rv0193chypothetical protein Rv0193c1.440.10067
Rv0194hypothetical protein Rv01941.040.40536
Rv0195hypothetical protein Rv01951.270.24536
Rv0196hypothetical protein Rv01961.070.23444
Rv0197hypothetical protein Rv01973.140.00644
Rv0198chypothetical protein Rv0198c1.220.02295
Rv0199hypothetical protein Rv01991.500.14779
Rv0200hypothetical protein Rv02001.120.24937
Rv0201chypothetical protein Rv0201c1.060.35582
Rv0202cmmpL110.870.20441
Rv0203hypothetical protein Rv02031.070.26790
Rv0204chypothetical protein Rv0204c2.790.00217
Rv0205hypothetical protein Rv02051.440.02955
Rv0206cmmpL31.960.08076
Rv0207chypothetical protein Rv0207c0.930.31878
Rv0208chypothetical protein Rv0208c1.060.41338
Rv0209hypothetical protein Rv02090.970.36805
Rv0210hypothetical protein Rv02101.480.02479
Rv0211pckA2.580.00000
Rv0212cnadR0.870.21020
Rv0213chypothetical protein Rv0213c1.620.05259
Rv0214fadD41.380.07466
Rv0215cfadE30.790.01473
Rv0216hypothetical protein Rv02162.250.00465
Rv0217clipW2.690.04463
Rv0218hypothetical protein Rv02181.390.00816
Rv0219hypothetical protein Rv02192.360.01607
Rv0220lipC0.860.14786
Rv0221hypothetical protein Rv02211.060.31824
Rv0222echA11.320.16352
Rv0223chypothetical protein Rv0223c3.830.01541
Rv0224chypothetical protein Rv0224c0.920.10697
Rv0225hypothetical protein Rv02252.680.06529
Rv0226chypothetical protein Rv0226c1.240.07132
Rv0227chypothetical protein Rv0227c1.130.25885
Rv0228hypothetical protein Rv02283.550.03895
Rv0229chypothetical protein Rv0229c2.450.27827
Rv0230chypothetical protein Rv0230c1.260.23287
Rv0231fadE41.880.04610
Rv0232hypothetical protein Rv02321.740.18288
Rv0233nrdB1.050.42057
Rv0234cgabD23.580.09771
Rv0235chypothetical protein Rv0235c2.540.06671
Rv0236chypothetical protein Rv0236c0.750.04156
Rv0237lpqI1.990.09071
Rv0238hypothetical protein Rv02382.180.01391
Rv0239hypothetical protein Rv02390.960.43430
Rv0240hypothetical protein Rv02401.390.19817
Rv0241chypothetical protein Rv0241c0.750.00973
Rv0242cfabG40.870.10981
Rv0243fadA20.660.00002
Rv0244cfadE51.520.01495
Rv0245hypothetical protein Rv02451.070.29100
Rv0246hypothetical protein Rv02461.560.03617
Rv0247chypothetical protein Rv0247c1.100.31282
Rv0248chypothetical protein Rv0248c0.810.10219
Rv0249chypothetical protein Rv0249c1.440.10930
Rv0250chypothetical protein Rv0250c1.540.02343
Rv0251chsp3.850.00392
Rv0252nirB1.000.49233
Rv0253nirD0.830.17040
Rv0254ccobU0.730.00125
Rv0255ccobQ2.240.00000
Rv0256cPPE1.880.04945
Rv0257chypothetical protein Rv0257c2.330.01242
Rv0258chypothetical protein Rv0258c2.020.02580
Rv0259chypothetical protein Rv0259c2.470.01552
Rv0260chypothetical protein Rv0260c2.450.00602
Rv0261cnarK32.150.00747
Rv0262chypothetical protein Rv0262c2.220.01359
Rv0263chypothetical protein Rv0263c1.680.01253
Rv0264chypothetical protein Rv0264c1.730.00913
Rv0265cfecB22.330.01438
Rv0266chypothetical protein Rv0266c0.960.34521
Rv0267narU1.560.00017
Rv0268chypothetical protein Rv0268c1.000.49561
Rv0269chypothetical protein Rv0269c2.210.01347
Rv0270fadD20.800.10075
Rv0271cfadE61.200.09660
Rv0272chypothetical protein Rv0272c1.130.09933
Rv0273chypothetical protein Rv0273c1.760.02046
Rv0274hypothetical protein Rv02741.110.25855
Rv0275cfadD272.480.00227
Rv0276hypothetical protein Rv02761.170.19934
Rv0277chypothetical protein Rv0277c1.290.07920
Rv0278cPE_PGRS1.740.08196
Rv0279cPE_PGRS6.240.04026
Rv0280PPE1.550.33659
Rv0281hypothetical protein Rv02811.540.03886
Rv0282hypothetical protein Rv02822.240.00127
Rv0283hypothetical protein Rv02831.150.09714
Rv0284hypothetical protein Rv02842.010.00079
Rv0285PE1.570.00294
Rv0286PPE3.090.03992
Rv0287hypothetical protein Rv02872.200.00042
Rv0288hypothetical protein Rv02882.850.00359
Rv0289hypothetical protein Rv02891.320.00829
Rv0290hypothetical protein Rv02901.220.09494
Rv0291hypothetical protein Rv02910.830.00000
Rv0292hypothetical protein Rv02921.320.06654
Rv0293chypothetical protein Rv0293c1.200.01285
Rv0294hypothetical protein Rv02940.800.01812
Rv0295chypothetical protein Rv0295c1.430.09367
Rv0296catsG1.630.06785
Rv0297PE_PGRS1.100.17676
Rv0298hypothetical protein Rv02980.630.00013
Rv0299hypothetical protein Rv02990.930.18312
Rv0300hypothetical protein Rv03001.010.48617
Rv0301hypothetical protein Rv03011.180.11050
Rv0302hypothetical protein Rv03020.600.00000
Rv0303hypothetical protein Rv03032.040.04198
Rv0304cPPE4.840.02911
Rv0305cPPE5.900.06182
Rv0306hypothetical protein Rv03061.620.05164
Rv0307chypothetical protein Rv0307c1.230.00424
Rv0308hypothetical protein Rv03081.230.03538
Rv0309hypothetical protein Rv03092.130.00430
Rv0310chypothetical protein Rv0310c1.010.43982
Rv0311hypothetical protein Rv03111.870.02787
Rv0312hypothetical protein Rv03121.290.15379
Rv0313hypothetical protein Rv03130.980.33951
Rv0314chypothetical protein Rv0314c0.540.00006
Rv0315hypothetical protein Rv03151.600.06993
Rv0316hypothetical protein Rv03161.880.04886
Rv0317cglpQ21.610.00336
Rv0318chypothetical protein Rv031830.760.00536
Rv0319pcp2.260.01617
Rv0320hypothetical protein Rv03200.630.00251
Rv0321dcd1.880.01377
Rv0322udgA1.020.41133
Rv0323chypothetical protein Rv0323c0.930.34427
Rv0324hypothetical protein Rv03242.410.00592
Rv0325hypothetical protein Rv03252.250.11709
Rv0326hypothetical protein Rv03261.050.41048
Rv0327chypothetical protein Rv0327c0.940.32114
Rv0328hypothetical protein Rv03280.750.00054
Rv0329chypothetical protein Rv0329c1.350.15773
Rv0330chypothetical protein Rv0330c2.640.02259
Rv0331hypothetical protein Rv03310.980.44778
Rv0332hypothetical protein Rv03320.990.45237
Rv0333hypothetical protein Rv03331.400.07186
Rv0334rmlA2.170.03880
Rv0335cPE1.330.12482
Rv0336hypothetical protein Rv03362.420.00956
Rv0337caspC1.150.01907
Rv0338chypothetical protein Rv0338c1.980.08029
Rv0339chypothetical protein Rv0339c2.300.03348
Rv0340hypothetical protein Rv03401.580.02116
Rv0341hypothetical protein Rv03410.880.01978
Rv0342hypothetical protein Rv03421.040.39340
Rv0343hypothetical protein Rv03431.220.06952
Rv0344clpqJ3.690.02948
Rv0345hypothetical protein Rv03450.860.12102
Rv0346caroP20.730.00058
Rv0347hypothetical protein Rv03471.760.02655
Rv0348hypothetical protein Rv03480.900.28542
Rv0349hypothetical protein Rv03491.430.02056
Rv0350dnaK1.620.00285
Rv0351grpE1.310.02918
Rv0352dnaJ0.930.06574
Rv0353hspR1.530.04134
Rv0354cPPE4.680.04355
Rv0355cPPE11.630.00847
Rv0356chypothetical protein Rv0356c0.840.03224
Rv0357cpurA1.630.01636
Rv0358hypothetical protein Rv03581.590.03809
Rv0359hypothetical protein Rv03591.700.00969
Rv0360chypothetical protein Rv0360c0.810.04112
Rv0361hypothetical protein Rv03615.320.02577
Rv0362mgtE0.700.00000
Rv0363cfba1.420.03549
Rv0364hypothetical protein Rv03641.060.35874
Rv0365chypothetical protein Rv0365c1.170.09675
Rv0366chypothetical protein Rv0366c2.050.02023
Rv0367chypothetical protein Rv0367c2.050.00227
Rv0368chypothetical protein Rv0368c1.700.08883
Rv0369chypothetical protein Rv0369c1.220.03243
Rv0370chypothetical protein Rv0370c0.610.00001
Rv0372chypothetical protein Rv0372c1.290.02082
Rv0373chypothetical protein Rv0373c0.810.05198
Rv0374chypothetical protein Rv0374c1.630.01124
Rv0375chypothetical protein Rv0375c1.120.19646
Rv0377hypothetical protein Rv03772.740.03920
Rv0379sec1.220.28895
Rv0381chypothetical protein Rv0381c1.030.40442
Rv0383chypothetical protein Rv0383c0.820.11270
Rv0384cclpB2.590.28260
Rv0385hypothetical protein Rv03851.670.00493
Rv0386hypothetical protein Rv03860.970.37845
Rv0387chypothetical protein Rv0387c8.090.00316
Rv0388cPPE3.500.00551
Rv0389purT1.960.00520
Rv0390hypothetical protein Rv03901.050.31849
Rv0391metZ1.180.08861
Rv0392chypothetical protein Rv0392c1.570.00059
Rv0393hypothetical protein Rv03931.200.03221
Rv0394chypothetical protein Rv0394c1.740.00239
Rv0395hypothetical protein Rv03952.920.03912
Rv0396hypothetical protein Rv03961.840.01146
Rv0397hypothetical protein Rv03971.820.01544
Rv0398chypothetical protein Rv0398c1.250.02453
Rv0399clpqK2.630.00613
Rv0400cfadE71.070.06665
Rv0401hypothetical protein Rv04010.950.33983
Rv0402cmmpL11.690.00454
Rv0403cmmpS13.340.02117
Rv0404fadD300.600.00000
Rv0405pks60.880.03039
Rv0406chypothetical protein Rv0406c2.220.00812
Rv0407hypothetical protein Rv04072.600.02748
Rv0408pta1.110.22268
Rv0409ackA0.910.14080
Rv0410cpknG1.690.00000
Rv0411cglnH1.810.00893
Rv0412chypothetical protein Rv0412c2.490.00003
Rv0413mutT30.820.11063
Rv0414cthiE1.860.00683
Rv0415hypothetical protein Rv04150.850.09021
Rv0416hypothetical protein Rv04161.130.00763
Rv0417thiG1.060.29521
Rv0418lpqL1.150.20553
Rv0419lpqM3.330.00422
Rv0420chypothetical protein Rv0420c1.610.01670
Rv0421chypothetical protein Rv0421c1.870.08628
Rv0422cthiD1.410.13995
Rv0423cthiC0.810.00443
Rv0424chypothetical protein Rv0424c0.630.00119
Rv0425cctpH0.890.00033
Rv0426chypothetical protein Rv0426c1.210.09791
Rv0427cxthA1.230.21266
Rv0428chypothetical protein Rv0428c5.370.03284
Rv0429cdef1.710.02653
Rv0430hypothetical protein Rv04300.780.06340
Rv0431hypothetical protein Rv04312.450.02703
Rv0432sodC0.890.32758
Rv0433hypothetical protein Rv04331.020.41288
Rv0434hypothetical protein Rv04341.080.19574
Rv0435chypothetical protein Rv0435c1.110.14439
Rv0436cpssA3.920.01146
Rv0437cpsd1.130.14594
Rv0438cmoaA31.850.00754
Rv0439chypothetical protein Rv0439c1.110.00538
Rv0440groEL23.570.00051
Rv0441chypothetical protein Rv0441c2.930.00087
Rv0442cPPE2.560.01579
Rv0443hypothetical protein Rv04431.060.28757
Rv0444chypothetical protein Rv0444c1.520.09453
Rv0445csigK0.810.03537
Rv0446chypothetical protein Rv0446c1.640.02923
Rv0447cufaA10.940.26571
Rv0448chypothetical protein Rv0448c1.240.00649
Rv0449chypothetical protein Rv0449c0.860.00087
Rv0450cmmpL41.290.04406
Rv0451cmmpS41.350.05462
Rv0452hypothetical protein Rv04521.300.10641
Rv0453PPE1.770.06300
Rv0454hypothetical protein Rv04541.970.01627
Rv0455chypothetical protein Rv0455c0.990.47987
Rv0456cechA20.820.09675
Rv0457chypothetical protein Rv0457c2.510.06141
Rv0458hypothetical protein Rv04581.640.11962
Rv0459hypothetical protein Rv04591.010.45005
Rv0460hypothetical protein Rv04601.260.03249
Rv0461hypothetical protein Rv04611.190.04873
Rv0462hypothetical protein Rv04620.980.45599
Rv0463hypothetical protein Rv04631.410.00547
Rv0464chypothetical protein Rv0464c1.560.04183
Rv0465chypothetical protein Rv0465c3.600.00528
Rv0466hypothetical protein Rv04660.940.28593
Rv0467aceA1.520.00428
Rv0468fadB20.850.00925
Rv0469umaA11.500.08759
Rv0470cumaA21.870.10337
Rv0471chypothetical protein Rv0471c3.680.03200
Rv0472chypothetical protein Rv0472c2.110.08836
Rv0473hypothetical protein Rv04732.050.01862
Rv0474hypothetical protein Rv04741.040.44613
Rv0475hypothetical protein Rv04750.820.00632
Rv0476hypothetical protein Rv04760.920.22391
Rv0477hypothetical protein Rv04771.000.47248
Rv0478deoC0.920.12372
Rv0479chypothetical protein Rv0479c0.910.38367
Rv0480chypothetical protein Rv0480c0.790.01570
Rv0481chypothetical protein Rv0481c3.580.01507
Rv0482murB1.440.00020
Rv0483hypothetical protein Rv04832.090.00509
Rv0484chypothetical protein Rv0484c1.480.00653
Rv0485hypothetical protein Rv04851.410.00000
Rv0486hypothetical protein Rv04860.990.39639
Rv0487hypothetical protein Rv04871.000.49272
Rv0488hypothetical protein Rv04881.510.01040
Rv0489gpm2.390.00038
Rv0490senX31.060.14565
Rv0491regX31.710.02021
Rv0492chypothetical protein Rv0492c1.710.00131
Rv0493chypothetical protein Rv0493c0.660.00348
Rv0494hypothetical protein Rv04943.110.01890
Rv0495chypothetical protein Rv0495c0.760.02044
Rv0496hypothetical protein Rv04960.780.00000
Rv0497hypothetical protein Rv04971.230.03663
Rv0498hypothetical protein Rv04981.180.26358
Rv0499hypothetical protein Rv04990.930.18791
Rv0500proC0.910.19831
Rv0501galE10.920.19813
Rv0502hypothetical protein Rv05021.310.23169
Rv0503ccmaA22.040.04420
Rv0504chypothetical protein Rv0504c1.920.02445
Rv0505cserB2.540.01103
Rv0506mmpS21.720.06144
Rv0507mmpL22.150.02694
Rv0508hypothetical protein Rv05080.870.28710
Rv0509hemA0.790.04491
Rv0510hemC0.550.00740
Rv0511cysG2.020.02601
Rv0512hemB0.750.02125
Rv0513hypothetical protein Rv05130.920.32960
Rv0514hypothetical protein Rv05141.060.33463
Rv0515hypothetical protein Rv05153.790.00602
Rv0516chypothetical protein Rv0516c0.880.02097
Rv0517hypothetical protein Rv05170.890.05898
Rv0518hypothetical protein Rv05181.120.18740
Rv0519chypothetical protein Rv0519c2.370.03368
Rv0520hypothetical protein Rv05201.660.00455
Rv0521chypothetical protein Rv0521c0.720.00007
Rv0522gabP1.530.02282
Rv0523chypothetical protein Rv0523c1.590.08362
Rv0524hemL0.600.00012
Rv0525hypothetical protein Rv05250.960.36495
Rv0526hypothetical protein Rv05261.580.17058
Rv0527ccsA0.790.00086
Rv0528hypothetical protein Rv05280.830.05421
Rv0529ccsB1.080.22810
Rv0530hypothetical protein Rv05301.440.00223
Rv0531hypothetical protein Rv05311.220.16656
Rv0532PE_PGRS1.320.11756
Rv0533cfabH1.410.01893
Rv0534cmenA1.180.27854
Rv0535pnp2.290.04354
Rv0536galE21.350.00401
Rv0537chypothetical protein Rv0537c2.630.00160
Rv0538hypothetical protein Rv05382.690.00167
Rv0539hypothetical protein Rv05391.220.00486
Rv0540hypothetical protein Rv05401.750.00001
Rv0541chypothetical protein Rv0541c1.050.32493
Rv0542cmenE0.830.23413
Rv0543chypothetical protein Rv0543c0.990.46086
Rv0544chypothetical protein Rv0544c0.970.41609
Rv0545cpitA0.710.01103
Rv0546chypothetical protein Rv0546c1.250.23549
Rv0547chypothetical protein Rv0547c1.840.02729
Rv0548cmenB2.910.02124
Rv0549chypothetical protein Rv0549c2.070.00096
Rv0550chypothetical protein Rv0550c1.070.26538
Rv0551cfadD81.190.09898
Rv0552hypothetical protein Rv05521.200.06050
Rv0553menC0.920.23410
Rv0554bpoC0.680.01874
Rv0555menD1.830.03192
Rv0556hypothetical protein Rv05560.900.31135
Rv0557hypothetical protein Rv05571.010.44915
Rv0558ubiE0.810.00077
Rv0559chypothetical protein Rv0559c1.060.16921
Rv0560chypothetical protein Rv0560c2.650.02135
Rv0561chypothetical protein Rv0561c1.540.00176
Rv0562grcC10.820.08827
Rv0563htpX1.010.47742
Rv0564cgpdA10.710.00000
Rv0565chypothetical protein Rv0565c2.730.04709
Rv0566chypothetical protein Rv0566c1.660.08557
Rv0567hypothetical protein Rv05671.850.02441
Rv0568hypothetical protein Rv05680.690.00006
Rv0569hypothetical protein Rv05694.940.00729
Rv0570nrdZ1.960.00000
Rv0571chypothetical protein Rv0571c0.930.16249
Rv0572chypothetical protein Rv0572c2.170.00163
Rv0573chypothetical protein Rv0573c1.930.00743
Rv0574chypothetical protein Rv0574c1.850.00203
Rv0575chypothetical protein Rv0575c1.490.01878
Rv0576hypothetical protein Rv05761.210.17056
Rv0577hypothetical protein Rv05771.240.09758
Rv0578cPE_PGRS0.910.15960
Rv0579hypothetical protein Rv05790.930.16288
Rv0580chypothetical protein Rv0580c0.920.21143
Rv0581hypothetical protein Rv05811.130.13672
Rv0582hypothetical protein Rv05821.090.25178
Rv0583clpqN1.090.30931
Rv0584hypothetical protein Rv05841.160.23259
Rv0585chypothetical protein Rv0585c0.840.04254
Rv0586hypothetical protein Rv05861.320.02539
Rv0587hypothetical protein Rv05871.250.12852
Rv0588hypothetical protein Rv05882.320.00343
Rv0589mce21.720.02870
Rv0590hypothetical protein Rv05901.080.27477
Rv0591hypothetical protein Rv05912.930.02030
Rv0592hypothetical protein Rv05924.760.00164
Rv0593lprL1.040.28300
Rv0594hypothetical protein Rv05941.460.02538
Rv0595chypothetical protein Rv0595c1.490.01260
Rv0596chypothetical protein Rv0596c0.990.44744
Rv0597chypothetical protein Rv0597c1.410.12338
Rv0598chypothetical protein Rv0598c2.040.02385
Rv0599chypothetical protein Rv0599c1.420.02601
Rv0600chypothetical protein Rv0600c1.690.01625
Rv0601chypothetical protein Rv0601c4.140.02187
Rv0602ctcrA1.590.02480
Rv0603hypothetical protein Rv06031.210.03274
Rv0604lpqO1.020.35319
Rv0605hypothetical protein Rv06051.250.06168
Rv0606hypothetical protein Rv06060.920.22309
Rv0607hypothetical protein Rv06071.630.00186
Rv0608hypothetical protein Rv06081.620.04827
Rv0609hypothetical protein Rv06091.670.00273
Rv0610chypothetical protein Rv0610c1.240.16771
Rv0611chypothetical protein Rv0611c1.020.41560
Rv0612hypothetical protein Rv06120.990.46182
Rv0613chypothetical protein Rv0613c1.780.14770
Rv0614hypothetical protein Rv06140.990.46783
Rv0615hypothetical protein Rv06151.390.06441
Rv0616chypothetical protein Rv0616c1.700.00321
Rv0617hypothetical protein Rv06174.130.08337
Rv0618galT′1.580.17496
Rv0619galT0.870.04564
Rv0620galK1.860.09685
Rv0621hypothetical protein Rv06212.220.02435
Rv0622hypothetical protein Rv06222.420.02745
Rv0623hypothetical protein Rv06232.510.00560
Rv0624hypothetical protein Rv06242.170.03266
Rv0625chypothetical protein Rv0625c1.470.09723
Rv0626hypothetical protein Rv06261.350.01001
Rv0627hypothetical protein Rv06271.120.23594
Rv0628chypothetical protein Rv0628c1.870.00249
Rv0629crecD0.990.47070
Rv0630crecB1.720.00811
Rv0631crecC1.870.07661
Rv0632cechA31.140.05891
Rv0633chypothetical protein Rv0633c5.800.09837
Rv0634chypothetical protein Rv0634c1.010.42720
Rv0635hypothetical protein Rv06350.710.00001
Rv0636hypothetical protein Rv06360.720.00096
Rv0637hypothetical protein Rv06370.980.44924
Rv0638secE1.050.35305
Rv0639nusG1.150.19532
Rv0640rplK1.010.43122
Rv0641rplA0.850.01986
Rv0642cmmaA41.090.40058
Rv0643cmmaA31.870.04225
Rv0644cmmaA20.660.00051
Rv0645cmmaA11.270.02866
Rv0646clipG0.840.01422
Rv0647chypothetical protein Rv0647c1.030.38837
Rv0648hypothetical protein Rv06483.080.00768
Rv0649fabD21.740.00787
Rv0650hypothetical protein Rv06501.080.04081
Rv0651rplJ1.240.07950
Rv0652rplL0.750.05830
Rv0653chypothetical protein Rv0653c2.520.01477
Rv0654hypothetical protein Rv06541.220.02754
Rv0655hypothetical protein Rv06550.780.17899
Rv0656chypothetical protein Rv0656c1.000.48461
Rv0657chypothetical protein Rv0657c1.430.05504
Rv0658chypothetical protein Rv0658c1.440.14414
Rv0659chypothetical protein Rv0659c1.180.20351
Rv0660chypothetical protein Rv0660c0.990.47866
Rv0661chypothetical protein Rv0661c2.280.05818
Rv0662chypothetical protein Rv0662c3.150.07117
Rv0663atsD1.950.08954
Rv0664hypothetical protein Rv06640.670.00050
Rv0665hypothetical protein Rv06651.240.26561
Rv0666hypothetical protein Rv06660.860.19477
Rv0667rpoB0.780.07167
Rv0668rpoC0.600.00017
Rv0669chypothetical protein Rv0669c1.460.16201
Rv0670end2.440.09477
Rv0671lpqP2.460.06178
Rv0672fadE81.600.12684
Rv0673echA40.880.08679
Rv0674hypothetical protein Rv06740.930.35229
Rv0675echA51.420.07488
Rv0676cmmpL52.190.02616
Rv0677cmmpS50.950.38635
Rv0678hypothetical protein Rv06781.870.00798
Rv0679chypothetical protein Rv0679c1.550.00988
Rv0680chypothetical protein Rv0680c0.910.34220
Rv0681hypothetical protein Rv06811.100.05407
Rv0682rpsL0.450.00007
Rv0683rpsG0.410.00000
Rv0684fusA0.570.00125
Rv0685tuf1.280.21090
Rv0686hypothetical protein Rv06861.020.45035
Rv0687hypothetical protein Rv06872.430.00365
Rv0688hypothetical protein Rv06880.920.12749
Rv0689chypothetical protein Rv0689c1.240.00873
Rv0690chypothetical protein Rv0690c1.020.46873
Rv0691chypothetical protein Rv0691c2.900.02105
Rv0692hypothetical protein Rv06920.180.00000
Rv0693pqqE0.450.00004
Rv0694lldD10.470.00004
Rv0695hypothetical protein Rv06956.870.13644
Rv0696hypothetical protein Rv06960.440.00051
Rv0697hypothetical protein Rv06971.430.08397
Rv0698hypothetical protein Rv06983.220.01617
Rv0699hypothetical protein Rv06991.070.32749
Rv0700rpsJ0.350.00000
Rv0701rplC0.450.00001
Rv0702rplD0.530.00391
Rv0703rplW0.410.00000
Rv0704rplB0.390.00021
Rv0705rpsS0.720.08317
Rv0706rplV0.430.00003
Rv0707rpsC0.780.00000
Rv0708rplP0.420.00039
Rv0709rpmC0.660.13699
Rv0710rpsQ0.470.00095
Rv0711atsA1.050.42694
Rv0712hypothetical protein Rv07122.030.06708
Rv0713hypothetical protein Rv07130.720.00130
Rv0714rplN3.010.06161
Rv0715rplX0.680.08267
Rv0716rplE0.420.00000
Rv0717rpsN0.860.34926
Rv0719rplF0.860.33463
Rv0720rplR1.520.08874
Rv0721rpsE0.840.04743
Rv0722rpmD0.450.00004
Rv0723rplO0.570.00011
Rv0724sppA0.980.42392
Rv0725chypothetical protein Rv0725c1.730.07006
Rv0726chypothetical protein Rv0726c1.180.01539
Rv0727cfucA2.600.00824
Rv0728chypothetical protein Rv0728c1.880.06336
Rv0729xylB0.980.43673
Rv0730hypothetical protein Rv07301.110.33131
Rv0731chypothetical protein Rv0731c1.960.03987
Rv0732secY1.700.18136
Rv0733adk0.620.00017
Rv0734map′0.500.00001
Rv0735sigL1.310.04305
Rv0736hypothetical protein Rv07360.800.08557
Rv0737hypothetical protein Rv07372.030.02063
Rv0738hypothetical protein Rv07380.680.00406
Rv0739hypothetical protein Rv07391.320.05574
Rv0740hypothetical protein Rv07401.030.42070
Rv0741hypothetical protein Rv07411.540.07662
Rv0742PE_PGRS0.920.26700
Rv0743chypothetical protein Rv0743c1.080.00331
Rv0744chypothetical protein Rv0744c0.660.00542
Rv0745hypothetical protein Rv07450.800.04147
Rv0746PE_PGRS3.450.04764
Rv0747PE_PGRS7.330.02625
Rv0748hypothetical protein Rv07480.730.00051
Rv0749hypothetical protein Rv07491.120.23418
Rv0750hypothetical protein Rv07501.270.04241
Rv0751cmmsB1.120.12286
Rv0752cfadE90.760.07426
Rv0753cmmsA1.040.38964
Rv0754PE_PGRS1.050.34685
Rv0755cPPE1.090.19960
Rv0756chypothetical protein Rv0756c0.640.00033
Rv0757phoP0.870.02137
Rv0759chypothetical protein Rv0759c4.380.04744
Rv0761cadhB1.830.14165
Rv0763chypothetical protein Rv0763c1.630.17092
Rv0764chypothetical protein Rv0764c0.770.02008
Rv0765chypothetical protein Rv0765c1.100.28745
Rv0766chypothetical protein Rv0766c1.510.22874
Rv0767chypothetical protein Rv0767c0.990.47086
Rv0769hypothetical protein Rv07691.350.05923
Rv0770hypothetical protein Rv07701.280.05356
Rv0771hypothetical protein Rv07711.540.02230
Rv0772purD2.610.00338
Rv0773cggtA0.910.12548
Rv0774chypothetical protein Rv0774c1.750.02445
Rv0775hypothetical protein Rv07752.110.02125
Rv0776chypothetical protein Rv0776c1.340.00004
Rv0777purB0.910.02740
Rv0778hypothetical protein Rv07781.120.12886
Rv0779chypothetical protein Rv0779c1.080.04739
Rv0780purC1.080.29474
Rv0781ptrBb2.190.04833
Rv0782ptrBa2.000.00882
Rv0783chypothetical protein Rv0783c1.020.46985
Rv0784hypothetical protein Rv07842.100.02079
Rv0785hypothetical protein Rv07851.160.13423
Rv0786chypothetical protein Rv0786c1.160.24176
Rv0787hypothetical protein Rv07870.620.01198
Rv0788purQ0.930.23557
Rv0789chypothetical protein Rv0789c2.830.03030
Rv0790chypothetical protein Rv0790c3.990.00784
Rv0791chypothetical protein Rv0791c2.290.00199
Rv0792chypothetical protein Rv0792c1.850.00288
Rv0793hypothetical protein Rv07932.020.02183
Rv0794clpdB2.280.00781
Rv0795hypothetical protein Rv07951.500.10087
Rv0796hypothetical protein Rv07962.330.02857
Rv0797hypothetical protein Rv07973.010.01738
Rv0798chypothetical protein Rv0798c0.890.13472
Rv0799chypothetical protein Rv0799c1.490.10722
Rv0800pepC0.730.00999
Rv0801hypothetical protein Rv08011.120.17970
Rv0802chypothetical protein Rv0802c1.150.19254
Rv0803purL0.980.43052
Rv0804hypothetical protein Rv08040.890.20418
Rv0805hypothetical protein Rv08051.280.14958
Rv0806ccpsY1.370.00390
Rv0807hypothetical protein Rv08070.980.37674
Rv0808purF0.990.48634
Rv0809purM0.990.45253
Rv0810chypothetical protein Rv0810c0.990.46943
Rv0811chypothetical protein Rv0811c1.250.02402
Rv0812pabC1.430.15050
Rv0813chypothetical protein Rv0813c0.880.02638
Rv0814csseC20.630.00116
Rv0815ccysA20.910.24675
Rv0816cthiX2.240.02116
Rv0817chypothetical protein Rv0817c0.700.00001
Rv0818hypothetical protein Rv08181.240.11793
Rv0819hypothetical protein Rv08190.920.09184
Rv0820phoT1.920.01033
Rv0821cphoY20.770.00167
Rv0822chypothetical protein Rv0822c1.320.08487
Rv0823chypothetical protein Rv0823c3.080.02703
Rv0824cdesA11.370.00725
Rv0825chypothetical protein Rv0825c0.640.00000
Rv0826hypothetical protein Rv08261.410.00080
Rv0827chypothetical protein Rv0827c1.230.10806
Rv0828chypothetical protein Rv0828c3.690.00251
Rv0829hypothetical protein Rv08291.720.00547
Rv0830hypothetical protein Rv08301.850.01080
Rv0831chypothetical protein Rv0831c1.050.34167
Rv0832PE_PGRS1.310.02592
Rv0833PE_PGRS2.820.01917
Rv0834cPE_PGRS2.630.02795
Rv0835lpqQ0.900.18314
Rv0836chypothetical protein Rv0836c6.740.00420
Rv0837chypothetical protein Rv0837c9.840.02515
Rv0838lpqR2.100.01254
Rv0839hypothetical protein Rv08394.670.01997
Rv0840chypothetical protein Rv0840c1.170.22365
Rv0841chypothetical protein Rv0841c1.720.02504
Rv0842hypothetical protein Rv08421.480.02957
Rv0843hypothetical protein Rv08431.720.02709
Rv0844cnarL1.560.07729
Rv0845hypothetical protein Rv08452.100.03001
Rv0846chypothetical protein Rv0846c2.320.00750
Rv0847lpqS1.400.04277
Rv0848cysM33.340.04213
Rv0849hypothetical protein Rv08492.670.07392
Rv0850hypothetical protein Rv08503.570.01719
Rv0851chypothetical protein Rv0851c1.560.02427
Rv0852fadD160.970.40894
Rv0853cpdc1.040.13693
Rv0854hypothetical protein Rv08540.850.00549
Rv0855far0.840.00346
Rv0856hypothetical protein Rv08561.010.47509
Rv0857hypothetical protein Rv08571.190.17231
Rv0858chypothetical protein Rv0858c1.850.08286
Rv0859fadA0.810.06593
Rv0860fadB0.700.04421
Rv0861chypothetical protein Rv0861c1.020.39616
Rv0862chypothetical protein Rv0862c0.680.00003
Rv0863hypothetical protein Rv08631.000.49309
Rv0864moaC20.550.00000
Rv0865mog1.070.25703
Rv0866moaE21.030.36493
Rv0867chypothetical protein Rv0867c1.050.33107
Rv0868cmoaD21.220.16089
Rv0869cmoaA21.250.01537
Rv0870chypothetical protein Rv0870c1.300.00001
Rv0871cspB1.460.10784
Rv0872cPE_PGRS1.290.11193
Rv0873fadE102.390.00705
Rv0874chypothetical protein Rv0874c1.430.00117
Rv0875chypothetical protein Rv0875c1.170.03838
Rv0876chypothetical protein Rv0876c1.350.00320
Rv0877hypothetical protein Rv08770.710.00021
Rv0878cPPE0.890.07391
Rv0879chypothetical protein Rv0879c2.170.00275
Rv0880hypothetical protein Rv08800.720.00352
Rv0881hypothetical protein Rv08811.750.00279
Rv0882hypothetical protein Rv08820.750.00629
Rv0883chypothetical protein Rv0883c2.310.03460
Rv0884cserC1.380.00980
Rv0885hypothetical protein Rv08853.830.00000
Rv0886fprB1.350.00114
Rv0887chypothetical protein Rv0887c2.070.00739
Rv0888hypothetical protein Rv08881.490.11778
Rv0889ccitA0.850.05160
Rv0890chypothetical protein Rv0890c1.050.19575
Rv0891chypothetical protein Rv0891c1.100.15599
Rv0892hypothetical protein Rv08922.870.00238
Rv0893chypothetical protein Rv0893c1.240.03573
Rv0894hypothetical protein Rv08942.040.02818
Rv0895hypothetical protein Rv08951.560.02756
Rv0896gltA20.580.00000
Rv0897chypothetical protein Rv0897c2.130.00098
Rv0898chypothetical protein Rv0898c0.780.00553
Rv0899ompA1.620.01324
Rv0900hypothetical protein Rv09001.090.28061
Rv0901hypothetical protein Rv09011.470.13657
Rv0902chypothetical protein Rv0902c1.380.04965
Rv0903chypothetical protein Rv0903c1.270.06684
Rv0904caccD32.390.01308
Rv0905echA61.090.20956
Rv0906hypothetical protein Rv09061.150.12609
Rv0907hypothetical protein Rv09070.680.00203
Rv0908ctpE0.590.00255
Rv0909hypothetical protein Rv09090.630.00010
Rv0910hypothetical protein Rv09100.780.09756
Rv0911hypothetical protein Rv09111.160.30033
Rv0912hypothetical protein Rv09121.460.15219
Rv0913chypothetical protein Rv0913c1.230.00412
Rv0914chypothetical protein Rv0914c1.190.20338
Rv0915cPPE2.720.02457
Rv0916cPE1.350.04300
Rv0917betP1.430.09174
Rv0918hypothetical protein Rv09181.540.00565
Rv0919hypothetical protein Rv09190.880.16915
Rv0920chypothetical protein Rv0920c2.100.06212
Rv0921hypothetical protein Rv09210.700.00765
Rv0922hypothetical protein Rv09221.110.30667
Rv0923chypothetical protein Rv0923c2.410.00355
Rv0924cnramp1.220.09940
Rv0925chypothetical protein Rv0925c3.030.00707
Rv0926chypothetical protein Rv0926c0.730.00015
Rv0927chypothetical protein Rv0927c1.640.03314
Rv0928phoS21.150.17691
Rv0929pstC21.890.04650
Rv0930pstA11.430.06777
Rv0931cpknD1.140.27394
Rv0932cpstS0.610.00010
Rv0933pstB1.060.30190
Rv0934phoS10.880.31029
Rv0935pstC1.130.25955
Rv0936pstA21.840.09977
Rv0937chypothetical protein Rv0937c0.950.30151
Rv0938hypothetical protein Rv09380.940.34580
Rv0939hypothetical protein Rv09391.380.03800
Rv0940chypothetical protein Rv0940c0.860.01062
Rv0941chypothetical protein Rv0941c1.050.35071
Rv0942hypothetical protein Rv09423.270.00683
Rv0943chypothetical protein Rv0943c2.180.06148
Rv0944hypothetical protein Rv09440.820.06073
Rv0945hypothetical protein Rv09451.000.48054
Rv0946cpgi1.180.14165
Rv0947chypothetical protein Rv0947c1.670.02110
Rv0948chypothetical protein Rv0948c0.610.00540
Rv0949uvrD1.080.20092
Rv0950chypothetical protein Rv0950c1.090.22888
Rv0951sucC0.740.00211
Rv0952sucD0.680.00065
Rv0953chypothetical protein Rv0953c0.710.00047
Rv0954hypothetical protein Rv09540.920.27339
Rv0955hypothetical protein Rv09551.280.16423
Rv0956purN1.390.07753
Rv0957purH1.690.01060
Rv0958hypothetical protein Rv09581.200.14256
Rv0959hypothetical protein Rv09590.670.00134
Rv0960hypothetical protein Rv09600.660.00002
Rv0961hypothetical protein Rv09612.570.01257
Rv0962chypothetical protein Rv0962c2.100.00013
Rv0963chypothetical protein Rv0963c5.160.01500
Rv0964chypothetical protein Rv0964c1.610.00177
Rv0965chypothetical protein Rv0965c1.970.00226
Rv0966chypothetical protein Rv0966c4.560.00169
Rv0967hypothetical protein Rv09672.490.00646
Rv0968hypothetical protein Rv096826.610.04204
Rv0969ctpV2.450.01023
Rv0970hypothetical protein Rv097022.750.00880
Rv0971cechA71.560.00093
Rv0973caccA21.670.00683
Rv0974caccD22.680.03225
Rv0975cfadE135.020.03182
Rv0976chypothetical protein Rv0976c1.190.17643
Rv0977PE_PGRS3.990.01847
Rv0978cPE_PGRS5.120.00047
Rv0979chypothetical protein Rv0979c1.190.05179
Rv0980cPE_PGRS3.350.00934
Rv0981hypothetical protein Rv098136.330.01248
Rv0982hypothetical protein Rv09826.440.00833
Rv0983hypothetical protein Rv09834.580.04662
Rv0984moaB220.310.01409
Rv0985cmscL1.020.46125
Rv0986hypothetical protein Rv09861.840.04955
Rv0987hypothetical protein Rv09873.760.02001
Rv0988hypothetical protein Rv09886.970.00409
Rv0989cgrcC219.630.01630
Rv0990chypothetical protein Rv0990c2.220.04391
Rv0991chypothetical protein Rv0991c3.200.01170
Rv0992chypothetical protein Rv0992c5.350.08165
Rv0993galU1.030.39319
Rv0994moeA0.870.05246
Rv0995rimJ1.170.17350
Rv0996hypothetical protein Rv09961.490.00036
Rv0997hypothetical protein Rv09971.200.14410
Rv0998hypothetical protein Rv09980.850.00061
Rv0999hypothetical protein Rv09991.700.07768
Rv1000hypothetical protein Rv10000.960.29679
Rv1001arcA1.290.01722
Rv1002chypothetical protein Rv1002c1.400.23097
Rv1003hypothetical protein Rv10031.130.27889
Rv1004chypothetical protein Rv1004c0.750.00000
Rv1005cpabB0.980.44325
Rv1006hypothetical protein Rv10060.810.03776
Rv1007cmetS1.130.27356
Rv1008hypothetical protein Rv10081.100.31141
Rv1009hypothetical protein Rv10090.790.00006
Rv1010ksgA0.880.22534
Rv1011hypothetical protein Rv10111.560.01903
Rv1012hypothetical protein Rv10120.790.02015
Rv1013pks161.510.06001
Rv1014cpth1.430.00007
Rv1015crplY1.470.05258
Rv1016clpqT1.160.06412
Rv1017cprsA0.750.00000
Rv1018cglmU7.970.00196
Rv1019hypothetical protein Rv10190.850.03630
Rv1020mfd1.480.00410
Rv1021hypothetical protein Rv10211.640.08465
Rv1022lpqU1.120.30432
Rv1023eno1.240.12574
Rv1024hypothetical protein Rv10242.030.05660
Rv1025hypothetical protein Rv10251.120.25845
Rv1026hypothetical protein Rv10261.960.05906
Rv1027ckdpE0.950.25394
Rv1028ckdpD1.580.00266
Rv1029kdpA4.560.00093
Rv1030kdpB1.260.13577
Rv1031kdpC2.600.00529
Rv1032chypothetical protein Rv1032c2.960.00175
Rv1033chypothetical protein Rv1033c3.040.01341
Rv1034chypothetical protein Rv1034c1.580.14088
Rv1035chypothetical protein Rv1035c1.180.05436
Rv1036chypothetical protein Rv1036c1.540.15984
Rv1037chypothetical protein Rv1037c0.400.00000
Rv1038chypothetical protein Rv1038c0.410.00000
Rv1039cPPE5.410.01891
Rv1040cPE2.320.08717
Rv1041chypothetical protein Rv1041c3.050.01947
Rv1042chypothetical protein Rv1042c1.060.33500
Rv1043chypothetical protein Rv1043c1.910.04380
Rv1044hypothetical protein Rv10442.350.03435
Rv1045hypothetical protein Rv10450.710.00003
Rv1046chypothetical protein Rv1046c1.180.24543
Rv1047hypothetical protein Rv10472.100.03443
Rv1048chypothetical protein Rv1048c1.100.31782
Rv1049hypothetical protein Rv10494.170.01166
Rv1050hypothetical protein Rv10500.810.00590
Rv1051chypothetical protein Rv1051c2.050.04175
Rv1052hypothetical protein Rv10520.960.14745
Rv1053chypothetical protein Rv1053c1.950.06211
Rv1054hypothetical protein Rv10541.270.22304
Rv1055hypothetical protein Rv10551.410.20520
Rv1056hypothetical protein Rv10562.580.04409
Rv1057hypothetical protein Rv10570.900.17607
Rv1058fadD142.240.02797
Rv1059hypothetical protein Rv10590.920.14302
Rv1060hypothetical protein Rv10601.090.32719
Rv1061hypothetical protein Rv10611.570.08810
Rv1062hypothetical protein Rv10621.480.00011
Rv1063chypothetical protein Rv1063c1.330.06210
Rv1064clpqV1.320.16695
Rv1065hypothetical protein Rv10650.700.00106
Rv1066hypothetical protein Rv10661.470.03102
Rv1067cPE_PGRS1.310.00400
Rv1068cPE_PGRS1.050.37683
Rv1069chypothetical protein Rv1069c1.240.00937
Rv1070cechA82.110.08960
Rv1071cechA91.000.49670
Rv1072hypothetical protein Rv10720.900.31109
Rv1073hypothetical protein Rv10732.570.00000
Rv1074cfadA31.700.16498
Rv1075chypothetical protein Rv1075c3.040.00889
Rv1076lipU1.030.39071
Rv1077cysM20.910.21210
Rv1078pra0.520.00000
Rv1079metB0.760.00959
Rv1080cgreA0.660.00337
Rv1081chypothetical protein Rv1081c3.340.03267
Rv1082hypothetical protein Rv10821.210.16714
Rv1083hypothetical protein Rv10831.840.06768
Rv1084hypothetical protein Rv10840.710.00491
Rv1085chypothetical protein Rv1085c2.670.01000
Rv1086hypothetical protein Rv10860.660.00107
Rv1087PE_PGRS1.750.02267
Rv1088PE0.970.38865
Rv1089PE1.170.32466
Rv1090hypothetical protein Rv10900.760.01283
Rv1091PE_PGRS1.480.00927
Rv1092ccoaA2.010.03113
Rv1093glyA1.810.17215
Rv1094desA22.490.22808
Rv1095phoH20.880.18537
Rv1097chypothetical protein Rv1097c1.130.35097
Rv1098cfum5.420.13753
Rv1099chypothetical protein Rv1099c2.760.06091
Rv1100hypothetical protein Rv11001.470.10802
Rv1101chypothetical protein Rv1101c1.350.13170
Rv1102chypothetical protein Rv1102c2.070.24745
Rv1103chypothetical protein Rv1103c1.660.07487
Rv1104hypothetical protein Rv11041.480.09307
Rv1105hypothetical protein Rv11051.230.00353
Rv1106chypothetical protein Rv1106c1.300.17897
Rv1107cxseB0.870.14427
Rv1108cxseA0.830.14244
Rv1109chypothetical protein Rv1109c1.120.30060
Rv1110lytB′0.650.01082
Rv1111chypothetical protein Rv1111c2.300.05081
Rv1112hypothetical protein Rv11120.820.13545
Rv1113hypothetical protein Rv11130.770.01472
Rv1114hypothetical protein Rv11141.570.05439
Rv1115hypothetical protein Rv11151.410.00087
Rv1116hypothetical protein Rv11161.280.08533
Rv1117hypothetical protein Rv11171.050.15858
Rv1118chypothetical protein Rv1118c1.190.18449
Rv1119chypothetical protein Rv1119c1.150.10672
Rv1120chypothetical protein Rv1120c0.700.00174
Rv1121zwf1.330.04588
Rv1122gnd20.670.01574
Rv1123cbpoB3.010.02140
Rv1124ephC0.950.38794
Rv1125hypothetical protein Rv11251.720.01284
Rv1126chypothetical protein Rv1126c2.000.03184
Rv1127cppdK0.990.48109
Rv1128chypothetical protein Rv1128c3.400.00264
Rv1129chypothetical protein Rv1129c1.750.02576
Rv1130hypothetical protein Rv11305.760.01903
Rv1131gltA14.460.00449
Rv1132hypothetical protein Rv11321.660.07321
Rv1133cmetE0.570.00000
Rv1134hypothetical protein Rv11340.540.00002
Rv1135cPPE1.240.02337
Rv1136hypothetical protein Rv11360.700.00061
Rv1137chypothetical protein Rv1137c3.320.02465
Rv1138chypothetical protein Rv1138c2.010.06195
Rv1139chypothetical protein Rv1139c3.940.00254
Rv1140hypothetical protein Rv11402.490.06003
Rv1141cechA111.790.04200
Rv1143mcr1.690.11368
Rv1145hypothetical protein Rv11451.220.15221
Rv1147hypothetical protein Rv11470.720.00003
Rv1149hypothetical protein Rv11491.320.09472
Rv1150hypothetical protein Rv11500.960.36701
Rv1151chypothetical protein Rv1151c2.940.06813
Rv1152hypothetical protein Rv11520.280.29003
Rv1153comt1.630.04357
Rv1154chypothetical protein Rv1154c1.620.00029
Rv1155hypothetical protein Rv11551.440.08357
Rv1156hypothetical protein Rv11561.000.49335
Rv1157chypothetical protein Rv1157c1.330.00877
Rv1158chypothetical protein Rv1158c1.730.03002
Rv1159hypothetical protein Rv11590.970.38022
Rv1160mutT21.470.00049
Rv1161narG1.190.00647
Rv1162narH1.110.24754
Rv1163narJ1.280.00227
Rv1164narI1.550.02080
Rv1165hypothetical protein Rv11651.510.03391
Rv1166lpqW1.210.17597
Rv1167chypothetical protein Rv1167c1.820.00601
Rv1168cPPE1.360.05962
Rv1169cPE2.820.00373
Rv1170hypothetical protein Rv11704.490.01103
Rv1171hypothetical protein Rv11711.270.09437
Rv1172cPE1.470.02067
Rv1173hypothetical protein Rv11730.690.00000
Rv1174chypothetical protein Rv1174c0.740.00000
Rv1175cfadH1.180.09299
Rv1176chypothetical protein Rv1176c1.210.07698
Rv1177fdxC1.040.34657
Rv1178hypothetical protein Rv11780.920.32601
Rv1179chypothetical protein Rv1179c2.300.01039
Rv1180pks30.660.00000
Rv1181pks41.180.17140
Rv1182papA30.850.14339
Rv1183mmpL102.140.01295
Rv1184chypothetical protein Rv1184c1.000.49361
Rv1185cfadD210.830.09483
Rv1186chypothetical protein Rv1186c0.990.40699
Rv1187rocA4.670.01807
Rv1188hypothetical protein Rv11881.330.04688
Rv1189sigI1.560.03997
Rv1190hypothetical protein Rv11901.960.01655
Rv1191hypothetical protein Rv11911.200.02625
Rv1192hypothetical protein Rv11922.360.02835
Rv1193fadD361.000.48571
Rv1194chypothetical protein Rv1194c0.900.07512
Rv1195PE0.740.04791
Rv1196PPE0.530.00023
Rv1197hypothetical protein Rv11970.470.00000
Rv1198hypothetical protein Rv11980.410.00000
Rv1199chypothetical protein Rv1199c2.050.01200
Rv1200hypothetical protein Rv12000.860.10216
Rv1201chypothetical protein Rv1201c0.850.00295
Rv1202dapE1.640.00574
Rv1203chypothetical protein Rv1203c1.420.04351
Rv1204chypothetical protein Rv1204c4.220.00519
Rv1205hypothetical protein Rv12050.870.00676
Rv1206fadD61.320.00251
Rv1207folP20.820.00957
Rv1208hypothetical protein Rv12081.180.11495
Rv1209hypothetical protein Rv12091.140.22783
Rv1210tagA1.400.05103
Rv1211hypothetical protein Rv12110.720.00000
Rv1212chypothetical protein Rv1212c1.050.15968
Rv1213glgC1.570.00927
Rv1214cPE1.790.00808
Rv1215chypothetical protein Rv1215c1.640.01316
Rv1216chypothetical protein Rv1216c1.050.42413
Rv1217chypothetical protein Rv1217c0.930.31566
Rv1218chypothetical protein Rv1218c1.860.00245
Rv1219chypothetical protein Rv1219c1.120.06240
Rv1220chypothetical protein Rv1220c1.150.11186
Rv1221sigE1.680.00275
Rv1222hypothetical protein Rv12221.450.00326
Rv1223htrA1.010.47247
Rv1224hypothetical protein Rv12241.090.11084
Rv1225chypothetical protein Rv1225c2.540.02950
Rv1226chypothetical protein Rv1226c1.300.18667
Rv1227chypothetical protein Rv1227c2.310.02707
Rv1228lpqX1.010.45531
Rv1229cmrp2.760.02849
Rv1230chypothetical protein Rv1230c1.110.18723
Rv1231chypothetical protein Rv1231c1.580.00735
Rv1232chypothetical protein Rv1232c1.230.06505
Rv1233chypothetical protein Rv1233c1.070.37875
Rv1234hypothetical protein Rv12340.980.25551
Rv1235lpqY1.260.02925
Rv1236sugA1.150.18038
Rv1237sugB1.270.19436
Rv1238sugC0.620.00000
Rv1239ccorA1.050.14669
Rv1240mdh1.060.38997
Rv1241hypothetical protein Rv12411.110.27651
Rv1242hypothetical protein Rv12421.140.20161
Rv1243cPE_PGRS0.970.27560
Rv1244lpqZ1.010.47731
Rv1245chypothetical protein Rv1245c1.680.00556
Rv1246chypothetical protein Rv1246c1.200.05708
Rv1247chypothetical protein Rv1247c1.340.01653
Rv1248csucA1.140.27861
Rv1249chypothetical protein Rv1249c1.170.08872
Rv1250hypothetical protein Rv12500.870.19155
Rv1251chypothetical protein Rv1251c1.720.00000
Rv1252clprE1.160.06028
Rv1253deaD1.130.15285
Rv1254hypothetical protein Rv12540.910.10389
Rv1255chypothetical protein Rv1255c1.440.00089
Rv1256chypothetical protein Rv1256c1.010.46500
Rv1257chypothetical protein Rv1257c1.380.00046
Rv1258chypothetical protein Rv1258c1.470.00003
Rv1259hypothetical protein Rv12591.300.00045
Rv1260hypothetical protein Rv12601.420.05211
Rv1261chypothetical protein Rv1261c1.060.27062
Rv1262chypothetical protein Rv1262c0.950.31631
Rv1263amiB21.130.04430
Rv1264hypothetical protein Rv12641.100.24502
Rv1265hypothetical protein Rv12651.290.06979
Rv1266cpknH0.610.00053
Rv1267cembR1.170.04495
Rv1268chypothetical protein Rv1268c1.320.03004
Rv1269chypothetical protein Rv1269c1.000.48181
Rv1270clprA1.180.05115
Rv1271chypothetical protein Rv1271c1.500.00208
Rv1272chypothetical protein Rv1272c1.010.47595
Rv1273chypothetical protein Rv1273c1.440.06701
Rv1274lprB0.810.06916
Rv1275lprC1.230.08088
Rv1276chypothetical protein Rv1276c0.920.01917
Rv1277hypothetical protein Rv12772.090.00743
Rv1278hypothetical protein Rv12781.550.00072
Rv1279hypothetical protein Rv12790.940.34059
Rv1280coppA4.130.09223
Rv1281coppD1.320.14671
Rv1282coppC1.270.00402
Rv1283coppB1.520.08792
Rv1285cysD1.300.07983
Rv1286cysN1.960.01479
Rv1287hypothetical protein Rv12871.460.01965
Rv1288hypothetical protein Rv12881.720.02510
Rv1289hypothetical protein Rv12891.130.26606
Rv1290chypothetical protein Rv1290c2.180.02815
Rv1291chypothetical protein Rv1291c2.280.01389
Rv1292argS1.300.16101
Rv1293lysA1.230.19155
Rv1294thrA1.010.46399
Rv1295thrC1.430.13946
Rv1296thrB1.080.31812
Rv1297rho0.500.00088
Rv1298rpmE0.670.02560
Rv1299prfA0.690.21114
Rv1300hemK0.880.13282
Rv1301hypothetical protein Rv13011.270.08049
Rv1302rfe2.520.00000
Rv1303hypothetical protein Rv13030.840.00231
Rv1304atpB0.300.00000
Rv1305atpE0.580.00000
Rv1306atpF0.300.00000
Rv1307atpH0.750.05475
Rv1308atpA0.450.00007
Rv1309atpG0.740.00000
Rv1310atpD0.260.00000
Rv1311atpC0.360.00000
Rv1312hypothetical protein Rv13120.490.00000
Rv1313chypothetical protein Rv1313c2.950.01266
Rv1314chypothetical protein Rv1314c0.930.14013
Rv1315murA1.160.18391
Rv1316cogt1.430.00668
Rv1317calkA3.540.02047
Rv1318chypothetical protein Rv1318c0.730.00922
Rv1319chypothetical protein Rv1319c1.900.04069
Rv1320chypothetical protein Rv1320c0.970.36934
Rv1321hypothetical protein Rv13211.030.37147
Rv1323fadA40.890.05672
Rv1324hypothetical protein Rv13240.750.17967
Rv1325cPE_PGRS1.200.10731
Rv1326cglgB1.220.06709
Rv1327chypothetical protein Rv1327c1.140.05132
Rv1329cdinG2.220.05022
Rv1330chypothetical protein Rv1330c1.340.08264
Rv1331hypothetical protein Rv13310.940.27341
Rv1332hypothetical protein Rv13321.240.16027
Rv1333hypothetical protein Rv13330.910.16613
Rv1334hypothetical protein Rv13341.050.40203
Rv1335hypothetical protein Rv13351.070.31108
Rv1336cysM1.250.21789
Rv1337hypothetical protein Rv13371.070.01368
Rv1338murI0.970.43099
Rv1339hypothetical protein Rv13390.990.48134
Rv1340rphA0.810.02853
Rv1341hypothetical protein Rv13410.930.32458
Rv1342cpks141.150.10015
Rv1343chypothetical protein Rv1343c1.810.00018
Rv1344hypothetical protein Rv13441.050.26597
Rv1345fadD330.830.00082
Rv1346fadE141.250.02857
Rv1347chypothetical protein Rv1347c1.160.06139
Rv1348hypothetical protein Rv13481.160.06511
Rv1349hypothetical protein Rv13491.070.30428
Rv1350fabG21.400.00094
Rv1351hypothetical protein Rv13510.990.43174
Rv1352hypothetical protein Rv13521.470.07423
Rv1353chypothetical protein Rv1353c1.760.11361
Rv1354chypothetical protein Rv1354c1.240.03439
Rv1355cmoeY1.430.10703
Rv1356chypothetical protein Rv1356c1.530.01321
Rv1357chypothetical protein Rv1357c1.410.03337
Rv1358hypothetical protein Rv13581.040.43616
Rv1359hypothetical protein Rv13591.110.23799
Rv1360hypothetical protein Rv13600.790.08190
Rv1361cPPE1.010.49071
Rv1362chypothetical protein Rv1362c0.820.00038
Rv1363chypothetical protein Rv1363c0.970.36222
Rv1364crsbU1.120.06520
Rv1365chypothetical protein Rv1365c1.640.00945
Rv1366hypothetical protein Rv13661.390.01767
Rv1367chypothetical protein Rv1367c2.360.00200
Rv1368lprF1.090.15258
Rv1369chypothetical protein Rv1369c1.930.01780
Rv1370chypothetical protein Rv1370c1.490.10347
Rv1371hypothetical protein Rv13711.410.02361
Rv1372pks181.750.01549
Rv1373hypothetical protein Rv13731.760.01780
Rv1374chypothetical protein Rv1374c1.140.29068
Rv1375hypothetical protein Rv13752.300.00037
Rv1376hypothetical protein Rv13761.220.13627
Rv1377chypothetical protein Rv1377c1.090.21699
Rv1378chypothetical protein Rv1378c1.410.09196
Rv1379pyrR1.060.24224
Rv1380pyrB0.880.07050
Rv1382hypothetical protein Rv13820.720.00562
Rv1383carA2.560.01031
Rv1384carB0.740.00491
Rv1385pyrF0.950.15507
Rv1386PE0.950.38321
Rv1387PPE1.040.45197
Rv1388mIHF0.720.00414
Rv1389gmk1.290.07955
Rv1390hypothetical protein Rv13900.770.00195
Rv1392metK0.960.41771
Rv1393chypothetical protein Rv1393c3.530.00615
Rv1394chypothetical protein Rv1394c2.380.00061
Rv1395hypothetical protein Rv13951.870.05974
Rv1396cPE_PGRS1.030.44535
Rv1397chypothetical protein Rv1397c1.840.00257
Rv1398chypothetical protein Rv1398c1.060.40189
Rv1399clipH0.950.19086
Rv1400clipI1.440.12702
Rv1401hypothetical protein Rv14010.920.17814
Rv1402priA1.050.20788
Rv1403chypothetical protein Rv1403c0.860.04401
Rv1404hypothetical protein Rv14040.990.47058
Rv1405chypothetical protein Rv1405c2.900.00092
Rv1406fmt2.960.00365
Rv1407fmu1.370.00116
Rv1408rpe5.940.03224
Rv1409ribG1.340.00805
Rv1410chypothetical protein Rv1410c0.930.35595
Rv1411clprG0.890.06531
Rv1412ribC0.940.30926
Rv1413hypothetical protein Rv14131.200.09545
Rv1414hypothetical protein Rv14142.100.07565
Rv1415ribA20.980.32606
Rv1416ribH0.930.35412
Rv1417hypothetical protein Rv14171.170.19517
Rv1418lprH0.810.01369
Rv1419hypothetical protein Rv14192.850.01007
Rv1420uvrC1.740.09725
Rv1421hypothetical protein Rv14211.070.06854
Rv1422hypothetical protein Rv14221.580.12463
Rv1423hypothetical protein Rv14231.030.38849
Rv1424chypothetical protein Rv1424c1.120.24556
Rv1425hypothetical protein Rv14251.930.11914
Rv1426clipO0.760.00775
Rv1427cfadD121.350.03093
Rv1428chypothetical protein Rv1428c2.710.09460
Rv1429hypothetical protein Rv14291.950.06563
Rv1430PE1.240.06888
Rv1431hypothetical protein Rv14311.600.14703
Rv1432hypothetical protein Rv14324.530.06103
Rv1433hypothetical protein Rv14331.280.13343
Rv1434hypothetical protein Rv14341.570.00499
Rv1435chypothetical protein Rv1435c1.100.39899
Rv1436gap1.170.36478
Rv1437pgk0.840.22806
Rv1438tpi0.630.00108
Rv1439chypothetical protein Rv1439c1.410.08386
Rv1440secG0.880.22239
Rv1441cPE_PGRS1.300.04780
Rv1442bisC1.070.33727
Rv1443chypothetical protein Rv1443c1.310.02496
Rv1444chypothetical protein Rv1444c0.710.00054
Rv1445cdevB1.200.00567
Rv1446copcA0.960.36465
Rv1447czwf21.130.01429
Rv1448ctal0.350.00000
Rv1449ctkt1.020.43725
Rv1450cPE_PGRS0.950.38156
Rv1451ctaB0.780.00015
Rv1452cPE_PGRS1.760.17083
Rv1453hypothetical protein Rv14531.060.25315
Rv1454cqor0.670.00017
Rv1455hypothetical protein Rv14551.340.00155
Rv1456chypothetical protein Rv1456c1.010.47327
Rv1457chypothetical protein Rv1457c1.170.07969
Rv1458chypothetical protein Rv1458c0.890.05392
Rv1459chypothetical protein Rv1459c1.620.00064
Rv1460hypothetical protein Rv14601.870.00000
Rv1461hypothetical protein Rv14611.280.00738
Rv1462hypothetical protein Rv14621.760.00003
Rv1463hypothetical protein Rv14631.090.12217
Rv1464hypothetical protein Rv14641.540.00016
Rv1465hypothetical protein Rv14651.430.00655
Rv1466hypothetical protein Rv14660.800.01458
Rv1467cfadE151.300.01125
Rv1468cPE_PGRS0.820.07307
Rv1469ctpD1.600.00323
Rv1470trxA0.920.00542
Rv1471trxB2.700.01803
Rv1472echA120.830.00431
Rv1473hypothetical protein Rv14731.790.02413
Rv1474chypothetical protein Rv1474c0.640.00002
Rv1475cacn2.270.05720
Rv1476hypothetical protein Rv14761.020.46786
Rv1477hypothetical protein Rv14771.330.20057
Rv1479moxR1.180.29454
Rv1480hypothetical protein Rv14801.590.34899
Rv1481hypothetical protein Rv14811.050.40667
Rv1483fabG11.510.13917
Rv1485hemZ1.950.00820
Rv1487hypothetical protein Rv14870.920.19144
Rv1488hypothetical protein Rv14882.330.06373
Rv1489chypothetical protein Rv1489c2.450.07586
Rv1490hypothetical protein Rv14903.090.01141
Rv1491chypothetical protein Rv1491c1.390.03761
Rv1492mutA1.640.04838
Rv1493mutB2.380.00126
Rv1494hypothetical protein Rv14940.980.34713
Rv1495hypothetical protein Rv14951.520.03408
Rv1496hypothetical protein Rv14961.450.06861
Rv1497lipL1.560.10899
Rv1498chypothetical protein Rv1498c1.410.05947
Rv1499hypothetical protein Rv14992.720.00337
Rv1500hypothetical protein Rv15001.390.09154
Rv1501hypothetical protein Rv15011.520.03859
Rv1502hypothetical protein Rv15020.740.00006
Rv1503chypothetical protein Rv1503c1.030.40129
Rv1504chypothetical protein Rv1504c1.090.25132
Rv1505chypothetical protein Rv1505c1.050.18422
Rv1506chypothetical protein Rv1506c1.050.36955
Rv1507chypothetical protein Rv1507c1.100.27823
Rv1508chypothetical protein Rv1508c1.110.17632
Rv1509hypothetical protein Rv15091.240.10388
Rv1510hypothetical protein Rv15100.800.00004
Rv1511gmdA1.120.05207
Rv1512epiA0.990.48572
Rv1513hypothetical protein Rv15132.040.00987
Rv1514chypothetical protein Rv1514c0.630.00016
Rv1515chypothetical protein Rv1515c2.900.01081
Rv1516chypothetical protein Rv1516c1.800.03344
Rv1517hypothetical protein Rv15172.410.00441
Rv1518hypothetical protein Rv15180.930.19993
Rv1519hypothetical protein Rv15191.650.00684
Rv1520hypothetical protein Rv15200.720.00001
Rv1521fadD251.970.06446
Rv1522cmmpL121.590.10291
Rv1523hypothetical protein Rv15231.700.09471
Rv1524hypothetical protein Rv15242.020.03413
Rv1525wbbl20.770.00335
Rv1526chypothetical protein Rv1526c2.130.05358
Rv1527cpks51.960.01846
Rv1528cpapA43.200.29593
Rv1529fadD241.680.00347
Rv1530adh0.660.00065
Rv1531hypothetical protein Rv15310.810.03967
Rv1532chypothetical protein Rv1532c14.250.03868
Rv1533hypothetical protein Rv15331.100.24718
Rv1534hypothetical protein Rv15340.660.00051
Rv1535hypothetical protein Rv15350.700.00278
Rv1536ileS0.660.01394
Rv1537dinX0.960.39509
Rv1538cansA3.330.00319
Rv1539lspA1.090.22576
Rv1540hypothetical protein Rv15401.900.00068
Rv1541clprI0.800.00016
Rv1542cglbN1.170.04215
Rv1543hypothetical protein Rv15430.920.05148
Rv1544hypothetical protein Rv15440.970.38289
Rv1545hypothetical protein Rv15450.980.32444
Rv1546hypothetical protein Rv15460.960.35012
Rv1547dnaE11.200.18489
Rv1548cPPE1.320.00097
Rv1549fadD11′1.650.00969
Rv1550fadD111.060.31705
Rv1551plsB10.720.00368
Rv1552frdA2.510.02553
Rv1553frdB1.000.49240
Rv1554frdC1.610.01975
Rv1555frdD1.010.45856
Rv1556hypothetical protein Rv15562.400.00747
Rv1557mmpL61.000.48533
Rv1558hypothetical protein Rv15581.320.04460
Rv1559ilvA1.510.03460
Rv1560hypothetical protein Rv15601.450.03285
Rv1561hypothetical protein Rv15612.320.02333
Rv1562cglgZ1.600.03181
Rv1563cglgY1.400.10954
Rv1564cglgX1.310.06004
Rv1565chypothetical protein Rv1565c3.060.01050
Rv1566chypothetical protein Rv1566c0.970.39000
Rv1567chypothetical protein Rv1567c1.000.48824
Rv1568bioA1.330.03251
Rv1569bioF1.670.06271
Rv1570bioD1.870.00145
Rv1571hypothetical protein Rv15711.070.27120
Rv1572chypothetical protein Rv1572c2.940.00927
Rv1573hypothetical protein Rv15731.400.15465
Rv1574hypothetical protein Rv15741.380.07829
Rv1575hypothetical protein Rv15753.370.01664
Rv1576chypothetical protein Rv1576c0.750.04077
Rv1577chypothetical protein Rv1577c2.870.02350
Rv1578chypothetical protein Rv1578c1.530.08781
Rv1579chypothetical protein Rv1579c1.080.10273
Rv1580chypothetical protein Rv1580c1.530.10338
Rv1581chypothetical protein Rv1581c4.280.03061
Rv1582chypothetical protein Rv1582c3.000.02166
Rv1583chypothetical protein Rv1583c0.850.00371
Rv1584chypothetical protein Rv1584c1.160.23402
Rv1585chypothetical protein Rv1585c1.830.00988
Rv1586chypothetical protein Rv1586c1.870.02238
Rv1587chypothetical protein Rv1587c8.460.01030
Rv1588chypothetical protein Rv1588c1.760.00222
Rv1589bioB0.800.00245
Rv1590hypothetical protein Rv15901.130.07794
Rv1591hypothetical protein Rv15911.540.04048
Rv1592chypothetical protein Rv1592c2.390.00072
Rv1593chypothetical protein Rv1593c1.100.31019
Rv1594nadA1.030.43886
Rv1595nadB0.740.00352
Rv1596nadC0.880.17879
Rv1597hypothetical protein Rv15971.000.49264
Rv1598chypothetical protein Rv1598c0.920.21420
Rv1599hisD1.250.02885
Rv1600hisC1.790.00001
Rv1601hisB1.320.06025
Rv1602hisH1.480.02802
Rv1603hisA0.980.44595
Rv1604impA0.970.44701
Rv1605hisF1.410.08652
Rv1606hisI20.760.05123
Rv1607chaA0.780.00389
Rv1608cbcpB1.660.01811
Rv1609trpE1.010.45955
Rv1610hypothetical protein Rv16101.010.45364
Rv1611trpC0.820.10784
Rv1612trpB1.510.15330
Rv1613trpA1.030.45141
Rv1614lgt0.700.01885
Rv1615hypothetical protein Rv16151.010.48417
Rv1616hypothetical protein Rv16161.510.08484
Rv1617pykA1.150.27259
Rv1618tesB10.790.07842
Rv1619hypothetical protein Rv16191.470.08159
Rv1620ccydC1.850.12167
Rv1621ccydD2.000.05786
Rv1622ccydB2.030.07699
Rv1623cappC1.920.02903
Rv1624chypothetical protein Rv1624c2.670.01634
Rv1625chypothetical protein Rv1625c1.510.14636
Rv1626hypothetical protein Rv16260.570.00001
Rv1627chypothetical protein Rv1627c1.610.09058
Rv1628chypothetical protein Rv1628c0.900.33297
Rv1629polA1.210.16223
Rv1630rpsA0.690.03663
Rv1631hypothetical protein Rv16310.630.00000
Rv1632chypothetical protein Rv1632c1.810.09798
Rv1633uvrB1.660.00683
Rv1634hypothetical protein Rv16341.220.00981
Rv1635chypothetical protein Rv1635c1.080.15147
Rv1636hypothetical protein Rv16361.540.00004
Rv1637chypothetical protein Rv1637c1.270.00018
Rv1638uvrA2.240.00000
Rv1639chypothetical protein Rv1639c0.810.02847
Rv1640clysX1.510.09300
Rv1641infC0.740.00007
Rv1642rpmI0.570.05666
Rv1643rplT0.610.00778
Rv1644tsnR0.780.01645
Rv1645chypothetical protein Rv1645c1.180.24164
Rv1646PE1.270.27730
Rv1647hypothetical protein Rv16471.130.15421
Rv1648hypothetical protein Rv16480.830.16213
Rv1649pheS1.840.00194
Rv1650pheT0.940.37187
Rv1651cPE_PGRS1.940.01701
Rv1652argC0.830.07586
Rv1653argJ1.140.13383
Rv1654argB1.020.35719
Rv1655argD1.460.00414
Rv1656argF1.400.00287
Rv1657argR3.040.02277
Rv1658argG5.070.01503
Rv1659argH2.010.05138
Rv1660pks104.300.03167
Rv1661pks70.680.00501
Rv1662pks81.030.44321
Rv1663pks171.150.03195
Rv1664pks91.550.01526
Rv1665pks111.660.08933
Rv1666chypothetical protein Rv1666c3.580.13938
Rv1667chypothetical protein Rv1667c0.970.39557
Rv1669hypothetical protein Rv16690.870.04204
Rv1670hypothetical protein Rv16701.580.06119
Rv1671hypothetical protein Rv16710.970.28377
Rv1672chypothetical protein Rv1672c1.250.04618
Rv1673chypothetical protein Rv1673c2.660.03339
Rv1674chypothetical protein Rv1674c1.610.00289
Rv1675chypothetical protein Rv1675c5.900.04301
Rv1676hypothetical protein Rv16760.580.00000
Rv1677dsbF1.220.32562
Rv1678hypothetical protein Rv16780.850.16465
Rv1679fadE161.300.24356
Rv1680hypothetical protein Rv16801.250.18781
Rv1681moeX3.650.01577
Rv1682hypothetical protein Rv16820.900.29064
Rv1683hypothetical protein Rv16831.440.01226
Rv1684hypothetical protein Rv16841.320.16142
Rv1685chypothetical protein Rv1685c1.650.01999
Rv1686chypothetical protein Rv1686c0.840.21445
Rv1687chypothetical protein Rv1687c1.990.00002
Rv1688hypothetical protein Rv16881.650.04256
Rv1689tyrS1.060.28292
Rv1690lprJ1.030.45452
Rv1691hypothetical protein Rv16911.150.04402
Rv1692hypothetical protein Rv16921.030.36762
Rv1693hypothetical protein Rv16931.130.12706
Rv1694tlyA0.900.19435
Rv1695hypothetical protein Rv16951.110.28026
Rv1696recN0.650.00029
Rv1697hypothetical protein Rv16972.280.02200
Rv1698hypothetical protein Rv16980.950.40141
Rv1699pyrG1.570.00434
Rv1700hypothetical protein Rv17000.670.00114
Rv1701hypothetical protein Rv17011.800.00100
Rv1702chypothetical protein Rv1702c2.680.00226
Rv1703chypothetical protein Rv1703c1.560.16497
Rv1704ccycA0.750.02002
Rv1705cPPE1.780.01907
Rv1706cPPE0.590.00274
Rv1707hypothetical protein Rv17071.730.03950
Rv1708hypothetical protein Rv17080.820.11542
Rv1709hypothetical protein Rv17091.520.06575
Rv1710hypothetical protein Rv17101.610.11954
Rv1711hypothetical protein Rv17110.950.08753
Rv1712cmk0.970.45943
Rv1713hypothetical protein Rv17130.990.46990
Rv1714hypothetical protein Rv17141.940.20206
Rv1715fadB31.360.01365
Rv1716hypothetical protein Rv17160.650.00049
Rv1717hypothetical protein Rv17171.340.02055
Rv1718hypothetical protein Rv17180.820.01172
Rv1719hypothetical protein Rv17191.090.23389
Rv1720chypothetical protein Rv1720c0.990.43602
Rv1721chypothetical protein Rv1721c1.220.23990
Rv1722hypothetical protein Rv17223.030.01070
Rv1723hypothetical protein Rv17233.190.06086
Rv1724chypothetical protein Rv1724c1.730.05508
Rv1725chypothetical protein Rv1725c2.110.02778
Rv1726hypothetical protein Rv17261.350.03454
Rv1727hypothetical protein Rv17271.120.08564
Rv1728chypothetical protein Rv1728c1.080.31481
Rv1729chypothetical protein Rv1729c1.600.12238
Rv1730chypothetical protein Rv1730c3.540.00274
Rv1731gabD10.800.00159
Rv1732chypothetical protein Rv1732c1.400.00000
Rv1733chypothetical protein Rv1733c3.030.00426
Rv1734chypothetical protein Rv1734c0.950.38479
Rv1735chypothetical protein Rv1735c1.010.44572
Rv1736cnarX1.230.11838
Rv1737cnarK21.030.40641
Rv1738hypothetical protein Rv17381.280.18728
Rv1739chypothetical protein Rv1739c1.230.16163
Rv1740hypothetical protein Rv17401.080.29251
Rv1741hypothetical protein Rv17411.300.01158
Rv1742hypothetical protein Rv17425.420.03402
Rv1743pknE1.310.06227
Rv1744chypothetical protein Rv1744c0.980.46906
Rv1745chypothetical protein Rv1745c2.020.02135
Rv1746pknF1.290.00312
Rv1747hypothetical protein Rv17470.970.43449
Rv1748hypothetical protein Rv17481.200.03823
Rv1749chypothetical protein Rv1749c1.150.00036
Rv1750cfadD11.320.00909
Rv1751hypothetical protein Rv17510.870.01325
Rv1752hypothetical protein Rv17525.710.00428
Rv1753cPPE7.300.00541
Rv1754chypothetical protein Rv1754c1.510.18247
Rv1755cplcD1.040.22674
Rv1756chypothetical protein Rv1756c2.280.09355
Rv1757chypothetical protein Rv1757c3.140.05771
Rv1758hypothetical protein Rv17580.760.00431
Rv1759cPE_PGRS(wag22)3.030.00598
Rv1760hypothetical protein Rv17601.160.14968
Rv1761chypothetical protein Rv1761c1.550.03119
Rv1762chypothetical protein Rv1762c1.120.32369
Rv1763hypothetical protein Rv17632.030.10003
Rv1764hypothetical protein Rv17641.420.23067
Rv1765chypothetical protein Rv1765c2.010.28177
Rv1766hypothetical protein Rv17660.750.00008
Rv1767hypothetical protein Rv17674.450.08185
Rv1768PE_PGRS1.560.10717
Rv1770hypothetical protein Rv17700.860.03927
Rv1771hypothetical protein Rv17711.450.21240
Rv1772hypothetical protein Rv17720.500.00007
Rv1774hypothetical protein Rv17741.840.03025
Rv1775hypothetical protein Rv17751.680.16443
Rv1776chypothetical protein Rv1776c0.750.04044
Rv1777hypothetical protein Rv17772.860.03024
Rv1778chypothetical protein Rv1778c1.490.00977
Rv1779chypothetical protein Rv1779c1.680.03426
Rv1780hypothetical protein Rv17801.510.05890
Rv1781chypothetical protein Rv1781c0.930.26262
Rv1782hypothetical protein Rv17822.320.00023
Rv1783hypothetical protein Rv17831.680.00249
Rv1784hypothetical protein Rv17841.160.20960
Rv1785chypothetical protein Rv1785c1.060.37190
Rv1786hypothetical protein Rv17861.180.18164
Rv1787PPE5.900.10000
Rv1788PE2.600.09177
Rv1789PPE2.010.05061
Rv1790PPE1.600.00689
Rv1791PE1.640.04554
Rv1792hypothetical protein Rv17920.500.00000
Rv1793hypothetical protein Rv17930.620.01066
Rv1794hypothetical protein Rv17940.760.01059
Rv1795hypothetical protein Rv17951.090.26501
Rv1796hypothetical protein Rv17961.510.01291
Rv1797hypothetical protein Rv17970.860.14025
Rv1798hypothetical protein Rv17980.960.41037
Rv1799lppT1.330.04441
Rv1800PPE3.020.04637
Rv1801PPE2.790.00524
Rv1802PPE1.940.06717
Rv1803cPE_PGRS1.400.08648
Rv1804chypothetical protein Rv1804c1.260.10120
Rv1805chypothetical protein Rv1805c2.220.04238
Rv1806PE3.240.01184
Rv1807PPE1.950.00514
Rv1808PPE2.850.00340
Rv1809PPE2.940.00516
Rv1810hypothetical protein Rv18102.000.03038
Rv1811mgtC3.020.02923
Rv1812chypothetical protein Rv1812c0.970.43417
Rv1814hypothetical protein Rv18142.490.09120
Rv1815hypothetical protein Rv18153.130.07426
Rv1816hypothetical protein Rv18161.020.45055
Rv1818cPE_PGRS0.740.01793
Rv1819chypothetical protein Rv1819c1.780.03700
Rv1820ilvG0.880.07671
Rv1821secA21.140.43425
Rv1822pgsA21.490.02925
Rv1823hypothetical protein Rv18233.200.12384
Rv1824hypothetical protein Rv18241.530.04701
Rv1825hypothetical protein Rv18253.850.00250
Rv1826gcvH0.790.00590
Rv1827hypothetical protein Rv18270.790.06946
Rv1828hypothetical protein Rv18280.860.07848
Rv1829hypothetical protein Rv18291.300.15301
Rv1830hypothetical protein Rv18301.030.45623
Rv1831hypothetical protein Rv18311.710.00635
Rv1832gcvB1.620.00709
Rv1833chypothetical protein Rv1833c1.130.22309
Rv1834hypothetical protein Rv18341.030.37847
Rv1835chypothetical protein Rv1835c1.450.02225
Rv1836chypothetical protein Rv1836c0.550.00000
Rv1837cglcB1.140.15116
Rv1838chypothetical protein Rv1838c0.690.00033
Rv1839chypothetical protein Rv1839c1.340.02154
Rv1840cPE_PGRS1.200.17434
Rv1841chypothetical protein Rv1841c1.340.04746
Rv1842chypothetical protein Rv1842c0.630.00016
Rv1843cguaB10.780.00005
Rv1844cgnd0.980.46663
Rv1845chypothetical protein Rv1845c1.110.13530
Rv1846chypothetical protein Rv1846c0.810.17315
Rv1847hypothetical protein Rv18471.130.15175
Rv1848ureA0.870.08625
Rv1849ureB1.340.11255
Rv1850ureC1.320.23137
Rv1851ureF1.240.03955
Rv1852ureG1.030.23952
Rv1853ureD1.090.06865
Rv1854cndh1.530.05141
Rv1855chypothetical protein Rv1855c2.520.01409
Rv1856chypothetical protein Rv1856c1.610.22693
Rv1857modA1.040.38319
Rv1858modB0.920.09089
Rv1859modC1.510.02802
Rv1860modD0.720.00852
Rv1861hypothetical protein Rv18610.910.14070
Rv1862adhA1.480.43981
Rv1863chypothetical protein Rv1863c1.380.16088
Rv1865chypothetical protein Rv1865c1.400.19903
Rv1867hypothetical protein Rv18671.960.08716
Rv1868hypothetical protein Rv18681.360.43810
Rv1869chypothetical protein Rv1869c1.210.30395
Rv1870chypothetical protein Rv1870c1.020.48150
Rv1871chypothetical protein Rv1871c0.450.00018
Rv1872clldD20.410.00000
Rv1873hypothetical protein Rv18731.990.00876
Rv1874hypothetical protein Rv18740.810.02937
Rv1875hypothetical protein Rv18751.360.01282
Rv1876bfrA2.970.05670
Rv1877hypothetical protein Rv18771.870.05747
Rv1878glnA30.910.22621
Rv1879hypothetical protein Rv18790.820.02111
Rv1880chypothetical protein Rv1880c0.730.00045
Rv1881clppE1.690.08253
Rv1882chypothetical protein Rv1882c1.330.20662
Rv1883chypothetical protein Rv1883c1.080.33208
Rv1884chypothetical protein Rv1884c0.520.00000
Rv1885chypothetical protein Rv1885c1.110.24029
Rv1886cfbpB0.440.00005
Rv1887hypothetical protein Rv18870.760.04701
Rv1888chypothetical protein Rv1888c1.430.05233
Rv1889chypothetical protein Rv1889c1.420.00457
Rv1890chypothetical protein Rv1890c0.750.03183
Rv1891hypothetical protein Rv18911.080.27583
Rv1892hypothetical protein Rv18920.780.14377
Rv1893hypothetical protein Rv18931.490.00035
Rv1894chypothetical protein Rv1894c2.570.02893
Rv1895hypothetical protein Rv18951.110.22002
Rv1896chypothetical protein Rv1896c1.540.11087
Rv1897chypothetical protein Rv1897c1.970.00413
Rv1898hypothetical protein Rv18980.630.00226
Rv1899clppD1.900.00929
Rv1900clipJ1.420.16436
Rv1901cinA2.770.01932
Rv1902cnanT1.140.22817
Rv1903hypothetical protein Rv19031.990.01177
Rv1904hypothetical protein Rv19040.720.03529
Rv1905caao1.700.00147
Rv1906chypothetical protein Rv1906c0.670.00734
Rv1907chypothetical protein Rv1907c5.640.00357
Rv1908ckatG3.170.02620
Rv1909cfurA2.800.00351
Rv1910chypothetical protein Rv1910c1.250.06789
Rv1911clppC1.320.13293
Rv1912cfadB51.210.03685
Rv1913hypothetical protein Rv19131.210.14228
Rv1914chypothetical protein Rv1914c0.570.00008
Rv1915aceAa0.850.03945
Rv1916aceAb0.740.03592
Rv1917cPPE1.910.02588
Rv1918cPPE1.700.05546
Rv1919chypothetical protein Rv1919c1.010.47858
Rv1920hypothetical protein Rv19200.990.47475
Rv1921clppF1.310.10504
Rv1922hypothetical protein Rv19221.870.02998
Rv1923lipD1.640.01848
Rv1924chypothetical protein Rv1924c1.730.00044
Rv1925fadD310.750.00053
Rv1926chypothetical protein Rv1926c1.220.06210
Rv1927hypothetical protein Rv19270.830.14015
Rv1928chypothetical protein Rv1928c1.320.00527
Rv1929chypothetical protein Rv1929c1.080.08757
Rv1930chypothetical protein Rv1930c2.040.00077
Rv1931chypothetical protein Rv1931c1.550.05625
Rv1932tpx1.030.38933
Rv1933cfadE181.400.01154
Rv1934cfadE171.280.17671
Rv1935cechA131.410.03903
Rv1936hypothetical protein Rv19363.230.00639
Rv1937hypothetical protein Rv19371.050.28489
Rv1938ephB1.520.01770
Rv1939hypothetical protein Rv19391.640.01204
Rv1940ribA0.860.21619
Rv1941hypothetical protein Rv19411.720.01180
Rv1942chypothetical protein Rv1942c3.050.02583
Rv1943chypothetical protein Rv1943c1.180.26553
Rv1944chypothetical protein Rv1944c1.170.08913
Rv1945hypothetical protein Rv19455.830.02865
Rv1946clppG0.960.37767
Rv1947hypothetical protein Rv19471.800.07874
Rv1948chypothetical protein Rv1948c0.950.35248
Rv1949chypothetical protein Rv1949c1.990.02128
Rv1950chypothetical protein Rv1950c1.190.18547
Rv1951chypothetical protein Rv1951c1.880.02981
Rv1952hypothetical protein Rv19521.300.16240
Rv1953hypothetical protein Rv19532.410.02054
Rv1954chypothetical protein Rv1954c2.930.00651
Rv1955hypothetical protein Rv19552.920.02145
Rv1956hypothetical protein Rv19562.260.00089
Rv1957hypothetical protein Rv19571.220.16863
Rv1958chypothetical protein Rv1958c0.680.00016
Rv1959chypothetical protein Rv1959c1.080.34005
Rv1960chypothetical protein Rv1960c1.120.18595
Rv1961hypothetical protein Rv19611.490.10692
Rv1962chypothetical protein Rv1962c0.700.00000
Rv1963chypothetical protein Rv1963c0.950.39421
Rv1964hypothetical protein Rv19640.810.00697
Rv1965hypothetical protein Rv19651.330.15855
Rv1966mce31.710.05221
Rv1967hypothetical protein Rv19671.710.09890
Rv1968hypothetical protein Rv19681.820.07973
Rv1969hypothetical protein Rv19692.100.02570
Rv1970lprM3.140.00621
Rv1971hypothetical protein Rv19712.060.05069
Rv1972hypothetical protein Rv19723.150.00108
Rv1973hypothetical protein Rv19731.220.00674
Rv1974hypothetical protein Rv19742.910.02246
Rv1975hypothetical protein Rv19753.710.01994
Rv1976chypothetical protein Rv1976c1.770.02485
Rv1977hypothetical protein Rv19770.850.03215
Rv1978hypothetical protein Rv19781.570.01344
Rv1979chypothetical protein Rv1979c0.940.30727
Rv1980cmpt640.950.35869
Rv1981cnrdF3.470.00570
Rv1982chypothetical protein Rv1982c1.410.03835
Rv1983PE_PGRS3.620.02044
Rv1984chypothetical protein Rv1984c3.670.00897
Rv1985chypothetical protein Rv1985c1.590.01003
Rv1986hypothetical protein Rv19863.820.00338
Rv1987hypothetical protein Rv19872.220.02092
Rv1988hypothetical protein Rv19882.860.00518
Rv1989chypothetical protein Rv1989c1.500.00343
Rv1990chypothetical protein Rv1990c2.720.00014
Rv1991chypothetical protein Rv1991c1.300.05558
Rv1992cctpG2.310.00000
Rv1993chypothetical protein Rv1993c1.660.00047
Rv1994chypothetical protein Rv1994c0.970.39773
Rv1995hypothetical protein Rv19957.140.00904
Rv1996hypothetical protein Rv19961.600.07980
Rv1997ctpF13.160.00700
Rv1998chypothetical protein Rv1998c1.310.02228
Rv1999chypothetical protein Rv1999c4.420.01590
Rv2000hypothetical protein Rv20001.100.07829
Rv2001hypothetical protein Rv20013.480.01670
Rv2002fabG31.040.36926
Rv2003chypothetical protein Rv2003c1.730.00464
Rv2004chypothetical protein Rv2004c1.410.02812
Rv2005chypothetical protein Rv2005c2.220.00366
Rv2006otsB1.730.03839
Rv2007cfdxA3.870.06076
Rv2008chypothetical protein Rv2008c1.500.02231
Rv2009hypothetical protein Rv20091.450.04126
Rv2010hypothetical protein Rv20100.670.00049
Rv2011chypothetical protein Rv2011c1.420.09925
Rv2012hypothetical protein Rv20121.100.27344
Rv2013hypothetical protein Rv20131.250.01183
Rv2014hypothetical protein Rv20140.770.00096
Rv2015chypothetical protein Rv2015c2.210.00001
Rv2016hypothetical protein Rv20161.130.13223
Rv2017hypothetical protein Rv20174.920.01282
Rv2018hypothetical protein Rv20182.300.00000
Rv2019hypothetical protein Rv20191.360.03636
Rv2020chypothetical protein Rv2020c1.400.01749
Rv2021chypothetical protein Rv2021c1.990.00452
Rv2022chypothetical protein Rv2022c1.120.02442
Rv2023chypothetical protein Rv2023c1.930.04334
Rv2024chypothetical protein Rv2024c2.440.10083
Rv2025chypothetical protein Rv2025c1.060.11663
Rv2026chypothetical protein Rv2026c3.450.00170
Rv2027chypothetical protein Rv2027c3.110.02450
Rv2028chypothetical protein Rv2028c1.300.06884
Rv2029cpfkB1.540.00627
Rv2030chypothetical protein Rv2030c7.790.00003
Rv2031chspX24.960.00885
Rv2032hypothetical protein Rv20325.460.01284
Rv2033chypothetical protein Rv2033c1.810.00285
Rv2034hypothetical protein Rv20341.730.00021
Rv2035hypothetical protein Rv20351.170.11416
Rv2036hypothetical protein Rv20360.880.17905
Rv2037chypothetical protein Rv2037c1.020.43047
Rv2038chypothetical protein Rv2038c0.640.00000
Rv2039chypothetical protein Rv2039c1.390.00814
Rv2040chypothetical protein Rv2040c1.440.02980
Rv2041chypothetical protein Rv2041c1.380.02204
Rv2042chypothetical protein Rv2042c1.330.05580
Rv2043cpncA1.540.00005
Rv2044chypothetical protein Rv2044c0.990.47669
Rv2045clipT1.160.24791
Rv2046lppI0.910.24654
Rv2047chypothetical protein Rv2047c1.260.00251
Rv2048cpks120.770.03189
Rv2049chypothetical protein Rv2049c1.190.04441
Rv2050hypothetical protein Rv20501.770.01998
Rv2051chypothetical protein Rv2051c1.320.00002
Rv2052chypothetical protein Rv2052c1.050.34550
Rv2053chypothetical protein Rv2053c0.920.18854
Rv2054hypothetical protein Rv20540.850.13340
Rv2055crpsR22.580.00272
Rv2056crpsN24.290.00000
Rv2057crpmG5.920.00130
Rv2058crpmB21.950.02776
Rv2059hypothetical protein Rv20592.620.00032
Rv2060hypothetical protein Rv20601.130.10411
Rv2061chypothetical protein Rv2061c0.710.02102
Rv2062ccobN1.990.08279
Rv2063chypothetical protein Rv2063c1.450.02477
Rv2064cobG1.030.38870
Rv2065cobH1.130.01515
Rv2066cobI1.700.00109
Rv2067chypothetical protein Rv2067c2.180.04175
Rv2068cblaC2.000.00127
Rv2069sigC2.230.00382
Rv2070ccobK0.930.39080
Rv2071ccobM1.360.00580
Rv2072ccobL2.110.00442
Rv2073chypothetical protein Rv2073c1.090.28462
Rv2074hypothetical protein Rv20740.540.00001
Rv2075chypothetical protein Rv2075c1.720.00273
Rv2076chypothetical protein Rv2076c0.850.13633
Rv2077chypothetical protein Rv2077c0.990.46615
Rv2078hypothetical protein Rv20780.890.10992
Rv2079hypothetical protein Rv20791.160.12628
Rv2080lppJ3.280.03239
Rv2081chypothetical protein Rv2081c1.360.02098
Rv2082hypothetical protein Rv20820.800.17407
Rv2083hypothetical protein Rv20831.100.17312
Rv2084hypothetical protein Rv20840.890.04539
Rv2085hypothetical protein Rv20851.040.37481
Rv2086hypothetical protein Rv20861.180.21983
Rv2087hypothetical protein Rv20871.510.02896
Rv2088pknJ1.030.37533
Rv2089cpepE4.390.00486
Rv2090hypothetical protein Rv20900.830.04229
Rv2091chypothetical protein Rv2091c1.020.45201
Rv2092chelY0.950.33445
Rv2093chypothetical protein Rv2093c1.820.04539
Rv2094chypothetical protein Rv2094c0.630.00072
Rv2095chypothetical protein Rv2095c2.100.05750
Rv2096chypothetical protein Rv2096c0.620.00000
Rv2097chypothetical protein Rv2097c1.730.07454
Rv2098cPE_PGRS1.160.25685
Rv2099cPE2.140.01290
Rv2100hypothetical protein Rv21001.330.01697
Rv2101helZ1.300.26045
Rv2102hypothetical protein Rv21020.790.02918
Rv2103chypothetical protein Rv2103c2.120.09583
Rv2104chypothetical protein Rv2104c1.060.25429
Rv2105hypothetical protein Rv21051.000.49971
Rv2106hypothetical protein Rv21062.120.09068
Rv2107PE1.290.10681
Rv2108PPE1.060.37623
Rv2109cprcA1.610.20944
Rv2110cprcB0.590.00007
Rv2111chypothetical protein Rv2111c1.210.28126
Rv2112chypothetical protein Rv2112c0.700.00124
Rv2113hypothetical protein Rv21132.060.00327
Rv2114hypothetical protein Rv21142.060.00298
Rv2115chypothetical protein Rv2115c1.460.00432
Rv2116lppK1.500.00927
Rv2117hypothetical protein Rv21171.110.03952
Rv2118chypothetical protein Rv2118c0.850.13389
Rv2119hypothetical protein Rv21191.250.01859
Rv2120chypothetical protein Rv2120c0.950.34665
Rv2121chisG1.730.07733
Rv2122chisI3.390.00297
Rv2123PPE10.480.02159
Rv2124cmetH1.380.10374
Rv2125hypothetical protein Rv21251.430.06030
Rv2126cPE_PGRS1.240.00004
Rv2127ansP0.770.01430
Rv2128hypothetical protein Rv21280.850.01835
Rv2129chypothetical protein Rv2129c1.840.00636
Rv2130ccysS21.940.05262
Rv2131ccysQ2.430.00606
Rv2132hypothetical protein Rv21321.270.00663
Rv2133chypothetical protein Rv2133c1.650.02642
Rv2134chypothetical protein Rv2134c1.140.15478
Rv2135chypothetical protein Rv2135c1.430.06341
Rv2136chypothetical protein Rv2136c2.060.01659
Rv2137chypothetical protein Rv2137c0.770.16604
Rv2138lppL1.080.40877
Rv2139pyrD1.340.11151
Rv2140chypothetical protein Rv2140c1.340.12137
Rv2141cdapE21.930.00837
Rv2142chypothetical protein Rv2142c4.800.02637
Rv2143hypothetical protein Rv21432.760.01108
Rv2144chypothetical protein Rv2144c0.710.00108
Rv2146chypothetical protein Rv2146c1.650.02026
Rv2147chypothetical protein Rv2147c1.610.02241
Rv2148chypothetical protein Rv2148c0.910.22570
Rv2149cyfiH3.710.05042
Rv2150cftsZ1.870.16620
Rv2151cftsQ1.740.16848
Rv2152cmurC0.850.20588
Rv2153cmurG1.000.49542
Rv2154cftsW1.240.28155
Rv2155cmurD2.120.05573
Rv2156cmurX0.760.00194
Rv2158cmurE1.450.05226
Rv2159chypothetical protein Rv2159c1.120.30984
Rv2160chypothetical protein Rv2160c0.840.30152
Rv2161chypothetical protein Rv2161c1.070.35411
Rv2162cPE_PGRS1.550.03271
Rv2163cpbpB1.260.04704
Rv2164chypothetical protein Rv2164c1.160.25628
Rv2165chypothetical protein Rv2165c1.320.07172
Rv2166chypothetical protein Rv2166c3.000.00290
Rv2167chypothetical protein Rv2167c3.260.05412
Rv2168chypothetical protein Rv2168c1.580.10968
Rv2169chypothetical protein Rv2169c1.010.48696
Rv2170hypothetical protein Rv21703.910.00324
Rv2171lppM0.760.00129
Rv2172chypothetical protein Rv2172c1.180.13342
Rv2173idsA23.880.00346
Rv2174hypothetical protein Rv21741.420.04928
Rv2175chypothetical protein Rv2175c1.830.00962
Rv2176pknL1.820.00256
Rv2177chypothetical protein Rv2177c2.900.03053
Rv2178caroG1.170.07775
Rv2179chypothetical protein Rv2179c1.470.13985
Rv2180chypothetical protein Rv2180c1.910.00023
Rv2181hypothetical protein Rv21812.030.05073
Rv2182chypothetical protein Rv2182c1.600.00007
Rv2183chypothetical protein Rv2183c1.220.04687
Rv2184chypothetical protein Rv2184c0.810.09024
Rv2185chypothetical protein Rv2185c1.810.04347
Rv2186chypothetical protein Rv2186c1.550.09628
Rv2187fadD151.470.05234
Rv2188chypothetical protein Rv2188c0.740.00198
Rv2189chypothetical protein Rv2189c0.870.00011
Rv2190chypothetical protein Rv2190c0.510.00013
Rv2191hypothetical protein Rv21911.010.46720
Rv2192ctrpD1.490.16577
Rv2193ctaE0.770.00253
Rv2194qcrC1.970.15118
Rv2195qcrA0.510.00001
Rv2196qcrB0.570.01581
Rv2197chypothetical protein Rv2197c0.840.10335
Rv2198cmmpS30.780.09545
Rv2199chypothetical protein Rv2199c1.090.41037
Rv2200cctaC0.700.05223
Rv2201asnB1.490.12230
Rv2202ccbhK0.840.00216
Rv2203hypothetical protein Rv22031.550.18089
Rv2204chypothetical protein Rv2204c0.580.00252
Rv2205chypothetical protein Rv2205c1.280.12324
Rv2206hypothetical protein Rv22060.730.01190
Rv2207cobT0.980.47511
Rv2208cobS2.510.11411
Rv2209hypothetical protein Rv22091.000.49853
Rv2210cilvE0.750.00497
Rv2211cgcvT0.690.00009
Rv2212hypothetical protein Rv22121.880.01167
Rv2213pepB1.670.01534
Rv2214cephD0.650.00018
Rv2215sucB2.090.01180
Rv2216hypothetical protein Rv22160.920.33884
Rv2217lipB1.590.06818
Rv2218lipA1.080.28106
Rv2219hypothetical protein Rv22190.870.00341
Rv2220glnA10.610.00022
Rv2221cglnE1.230.08313
Rv2222cglnA20.720.01469
Rv2223chypothetical protein Rv2223c2.380.01647
Rv2224chypothetical protein Rv2224c0.640.00091
Rv2225panB2.100.00003
Rv2226hypothetical protein Rv22260.890.10499
Rv2227hypothetical protein Rv22272.540.00390
Rv2228chypothetical protein Rv2228c0.900.32675
Rv2229chypothetical protein Rv2229c1.640.04398
Rv2230chypothetical protein Rv2230c1.000.49350
Rv2231ccobC1.180.05200
Rv2232hypothetical protein Rv22322.660.03427
Rv2233hypothetical protein Rv22331.420.08668
Rv2234ptpA1.230.17460
Rv2235hypothetical protein Rv22352.900.03888
Rv2236ccobD0.890.04695
Rv2237hypothetical protein Rv22370.850.03769
Rv2238cahpE0.900.30819
Rv2239chypothetical protein Rv2239c1.090.21265
Rv2240chypothetical protein Rv2240c1.250.10737
Rv2241aceE0.880.08244
Rv2242hypothetical protein Rv22421.280.21860
Rv2243fabD1.120.17272
Rv2244acpM0.810.24418
Rv2245kasA0.960.43917
Rv2246kasB0.670.01915
Rv2247accD61.980.06107
Rv2248hypothetical protein Rv22482.840.17042
Rv2249cglpD11.640.00080
Rv2250chypothetical protein Rv2250c0.820.08353
Rv2251hypothetical protein Rv22510.810.00138
Rv2252hypothetical protein Rv22521.000.49595
Rv2253hypothetical protein Rv22531.210.22088
Rv2254chypothetical protein Rv2254c0.900.15366
Rv2255chypothetical protein Rv2255c1.000.49233
Rv2256chypothetical protein Rv2256c1.340.11402
Rv2257chypothetical protein Rv2257c1.970.05799
Rv2258chypothetical protein Rv2258c1.230.26464
Rv2259adhE20.990.46523
Rv2260hypothetical protein Rv22600.680.00035
Rv2261chypothetical protein Rv2261c0.950.31390
Rv2262chypothetical protein Rv2262c0.900.17111
Rv2263hypothetical protein Rv22630.930.32922
Rv2264chypothetical protein Rv2264c1.280.07714
Rv2265hypothetical protein Rv22651.560.05317
Rv2266hypothetical protein Rv22661.420.18137
Rv2267chypothetical protein Rv2267c0.940.33382
Rv2268chypothetical protein Rv2268c1.340.05949
Rv2269chypothetical protein Rv2269c3.130.00298
Rv2270lppN0.610.00129
Rv2271hypothetical protein Rv22710.850.09975
Rv2272hypothetical protein Rv22721.320.19302
Rv2273hypothetical protein Rv22735.050.00943
Rv2274chypothetical protein Rv2274c1.510.13481
Rv2275hypothetical protein Rv22754.270.01797
Rv2276hypothetical protein Rv22760.780.00703
Rv2277chypothetical protein Rv2277c1.390.00685
Rv2278hypothetical protein Rv22781.060.41954
Rv2279hypothetical protein Rv22792.430.01660
Rv2280hypothetical protein Rv22800.520.00010
Rv2281pitB1.300.00000
Rv2282chypothetical protein Rv2282c0.980.41009
Rv2283hypothetical protein Rv22831.130.00252
Rv2284lipM1.910.05263
Rv2285hypothetical protein Rv22851.360.00317
Rv2286chypothetical protein Rv2286c0.660.00070
Rv2287yjcE1.290.00542
Rv2288hypothetical protein Rv22880.930.27581
Rv2289cdh1.770.04718
Rv2290lppO4.630.03736
Rv2291sseB0.940.35218
Rv2292chypothetical protein Rv2292c2.190.03059
Rv2293chypothetical protein Rv2293c3.660.02575
Rv2294hypothetical protein Rv22940.800.15275
Rv2295hypothetical protein Rv22958.170.05320
Rv2296hypothetical protein Rv22960.610.00008
Rv2297hypothetical protein Rv22971.710.15563
Rv2298hypothetical protein Rv22980.750.02024
Rv2299chtpG3.200.05972
Rv2300chypothetical protein Rv2300c0.870.22693
Rv2301hypothetical protein Rv23011.160.35753
Rv2302hypothetical protein Rv23020.800.15004
Rv2303chypothetical protein Rv2303c2.660.03136
Rv2304chypothetical protein Rv2304c1.090.32832
Rv2305hypothetical protein Rv23051.230.04246
Rv2306chypothetical protein Rv2306c3.330.00169
Rv2307chypothetical protein Rv2307c1.170.13350
Rv2308hypothetical protein Rv23081.420.01864
Rv2309chypothetical protein Rv2309c1.180.20067
Rv2310hypothetical protein Rv23101.480.00001
Rv2311hypothetical protein Rv23110.810.01061
Rv2312hypothetical protein Rv23121.180.08105
Rv2313chypothetical protein Rv2313c0.780.00283
Rv2314chypothetical protein Rv2314c1.610.00972
Rv2315chypothetical protein Rv2315c0.740.01168
Rv2316uspA2.220.01590
Rv2317uspE0.760.02787
Rv2318uspC1.970.01498
Rv2319chypothetical protein Rv2319c1.740.08537
Rv2320crocE1.030.39593
Rv2321crocD21.170.01397
Rv2322crocD12.120.00958
Rv2323chypothetical protein Rv2323c4.780.03378
Rv2324hypothetical protein Rv23241.080.31364
Rv2325chypothetical protein Rv2325c0.880.09908
Rv2326chypothetical protein Rv2326c2.000.00796
Rv2327hypothetical protein Rv23271.840.01394
Rv2328PE2.700.04551
Rv2329cnarK11.890.04617
Rv2330clppP2.020.00449
Rv2331hypothetical protein Rv23310.990.46469
Rv2332mez1.040.29871
Rv2333chypothetical protein Rv2333c1.400.12314
Rv2334cysK1.120.38225
Rv2335cysE1.320.17968
Rv2336hypothetical protein Rv23361.310.12011
Rv2337chypothetical protein Rv2337c2.010.02652
Rv2338cmoeW2.000.04685
Rv2339mmpL91.420.10401
Rv2340cPE0.930.22982
Rv2341lppQ1.140.26260
Rv2342hypothetical protein Rv23421.290.11778
Rv2343cdnaG2.740.00073
Rv2344cdgt1.490.21042
Rv2345hypothetical protein Rv23452.160.08295
Rv2346chypothetical protein Rv2346c0.510.00000
Rv2347chypothetical protein Rv2347c0.490.00000
Rv2348chypothetical protein Rv2348c0.510.00068
Rv2349cplcC2.170.08134
Rv2350cplcB0.850.08433
Rv2351cplcA3.540.02280
Rv2352cPPE0.760.01017
Rv2353cPPE1.750.08025
Rv2354hypothetical protein Rv23541.570.10415
Rv2355hypothetical protein Rv23552.480.09685
Rv2356cPPE0.840.12558
Rv2357cglyS0.900.18071
Rv2358hypothetical protein Rv23581.840.01134
Rv2359furB0.910.03397
Rv2360chypothetical protein Rv2360c1.200.00271
Rv2361chypothetical protein Rv2361c1.350.08310
Rv2362chypothetical protein Rv2362c1.200.03519
Rv2363amiA21.690.13858
Rv2364cbex0.990.41490
Rv2365chypothetical protein Rv2365c1.100.01891
Rv2366chypothetical protein Rv2366c0.790.04180
Rv2367chypothetical protein Rv2367c1.190.15605
Rv2368cphoH1.320.01032
Rv2369chypothetical protein Rv2369c1.370.00409
Rv2370chypothetical protein Rv2370c3.950.00516
Rv2371PE0.900.02037
Rv2372chypothetical protein Rv2372c1.560.00838
Rv2373cdnaJ21.870.00143
Rv2374chrcA1.230.08721
Rv2375hypothetical protein Rv23751.850.01592
Rv2376chypothetical protein Rv2376c0.520.00002
Rv2377cmbtH2.120.03560
Rv2378cmbtG2.060.00147
Rv2379cmbtF1.510.00028
Rv2380cmbtE3.990.00720
Rv2381cmbtD3.150.05137
Rv2382cmbtC4.270.01205
Rv2383cmbtB5.040.01804
Rv2384mbtA1.680.02517
Rv2385lipK3.530.04556
Rv2386ctrpE21.180.10213
Rv2387hypothetical protein Rv23871.260.04676
Rv2388chemN0.630.00000
Rv2389chypothetical protein Rv2389c1.260.19292
Rv2390chypothetical protein Rv2390c0.860.00305
Rv2391nirA0.340.00000
Rv2392cysH0.460.00156
Rv2393hypothetical protein Rv23930.490.00512
Rv2394ggtB0.710.06190
Rv2395hypothetical protein Rv23951.040.42273
Rv2396PE_PGRS0.680.00058
Rv2397ccysA1.700.12695
Rv2398ccysW0.710.00210
Rv2399ccysT3.750.05881
Rv2400csubI1.230.14022
Rv2401hypothetical protein Rv24011.330.03746
Rv2402hypothetical protein Rv24021.040.38583
Rv2403clppR1.120.00500
Rv2404clepA1.580.02695
Rv2405hypothetical protein Rv24051.100.23855
Rv2406chypothetical protein Rv2406c0.980.44630
Rv2407hypothetical protein Rv24073.690.10612
Rv2408PE1.270.00000
Rv2409chypothetical protein Rv2409c1.100.20552
Rv2410chypothetical protein Rv2410c1.130.18418
Rv2411chypothetical protein Rv2411c2.460.00174
Rv2412rpsT1.010.47209
Rv2413chypothetical protein Rv2413c1.020.29491
Rv2414chypothetical protein Rv2414c0.950.32546
Rv2415chypothetical protein Rv2415c2.460.03389
Rv2416chypothetical protein Rv2416c1.490.00002
Rv2417chypothetical protein Rv2417c0.880.12899
Rv2418chypothetical protein Rv2418c1.010.47714
Rv2419chypothetical protein Rv2419c0.980.41793
Rv2420chypothetical protein Rv2420c0.670.00120
Rv2421chypothetical protein Rv2421c4.660.01912
Rv2422hypothetical protein Rv24220.900.06529
Rv2423hypothetical protein Rv24230.740.01182
Rv2424chypothetical protein Rv2424c1.950.02106
Rv2425chypothetical protein Rv2425c0.910.20372
Rv2426chypothetical protein Rv2426c0.820.00654
Rv2427cproA1.870.05161
Rv2428ahpC1.110.31339
Rv2429ahpD3.460.00157
Rv2430cPPE1.840.14131
Rv2431cPE0.800.00434
Rv2432chypothetical protein Rv2432c0.620.00028
Rv2433chypothetical protein Rv2433c1.030.37910
Rv2434chypothetical protein Rv2434c1.610.05569
Rv2435chypothetical protein Rv2435c1.030.40852
Rv2436rbsK1.310.06404
Rv2437hypothetical protein Rv24372.180.00133
Rv2438chypothetical protein Rv2438c1.060.39475
Rv2439cproB1.480.22648
Rv2440cobg0.590.00008
Rv2441crpmA0.830.16086
Rv2442crplU0.630.00288
Rv2443dctA1.020.44358
Rv2444crne0.630.00264
Rv2445cndkA0.830.25375
Rv2446chypothetical protein Rv2446c0.680.00096
Rv2447cfolC0.810.06509
Rv2448cvalS1.090.15899
Rv2449chypothetical protein Rv2449c0.970.30339
Rv2450chypothetical protein Rv2450c1.240.19442
Rv2451hypothetical protein Rv24512.980.01746
Rv2452chypothetical protein Rv2452c2.340.02241
Rv2453chypothetical protein Rv2453c1.350.01570
Rv2454chypothetical protein Rv2454c1.680.00003
Rv2455chypothetical protein Rv2455c2.380.00000
Rv2456chypothetical protein Rv2456c0.780.06663
Rv2457cclpX0.780.08205
Rv2458hypothetical protein Rv24582.450.03179
Rv2459hypothetical protein Rv24590.920.29479
Rv2460cclpP21.100.36087
Rv2461cclpP1.080.07240
Rv2462ctig0.740.00913
Rv2463lipP1.030.40134
Rv2464chypothetical protein Rv2464c1.210.06317
Rv2465crpi1.010.44818
Rv2466chypothetical protein Rv2466c0.660.00213
Rv2467pepD1.850.02781
Rv2468chypothetical protein Rv2468c0.980.46265
Rv2469chypothetical protein Rv2469c0.920.26635
Rv2470glbO0.790.00010
Rv2471hypothetical protein Rv24711.500.00057
Rv2472hypothetical protein Rv24721.240.26904
Rv2473hypothetical protein Rv24733.010.02369
Rv2474chypothetical protein Rv2474c0.660.00026
Rv2475chypothetical protein Rv2475c2.410.04336
Rv2476chypothetical protein Rv2476c1.070.33697
Rv2477chypothetical protein Rv2477c1.030.44447
Rv2478chypothetical protein Rv2478c0.810.00146
Rv2479chypothetical protein Rv2479c2.260.05002
Rv2480chypothetical protein Rv2480c1.010.46523
Rv2481chypothetical protein Rv2481c2.290.05958
Rv2482cplsB21.050.42399
Rv2483chypothetical protein Rv2483c1.680.02160
Rv2484chypothetical protein Rv2484c0.770.04231
Rv2485clipQ0.970.45586
Rv2486echA141.090.32693
Rv2487cPE_PGRS1.120.14068
Rv2488chypothetical protein Rv2488c1.210.15385
Rv2489chypothetical protein Rv2489c1.510.07173
Rv2490cPE_PGRS0.650.00508
Rv2491hypothetical protein Rv24912.700.05925
Rv2492hypothetical protein Rv24920.730.00001
Rv2493hypothetical protein Rv24931.520.11098
Rv2494hypothetical protein Rv24942.330.03838
Rv2495cpdhC1.010.48192
Rv2496cpdhB1.280.02409
Rv2497cpdhA2.040.00079
Rv2498ccitE1.190.07162
Rv2499chypothetical protein Rv2499c1.550.00371
Rv2500cfadE191.320.00009
Rv2501caccA10.940.36257
Rv2502caccD11.240.03456
Rv2503cscoB1.150.00225
Rv2504cscoA1.750.01215
Rv2505cfadD351.320.04651
Rv2506hypothetical protein Rv25061.280.13916
Rv2507hypothetical protein Rv25070.940.20747
Rv2508chypothetical protein Rv2508c0.990.48723
Rv2509hypothetical protein Rv25091.030.41555
Rv2510chypothetical protein Rv2510c0.950.36428
Rv2511hypothetical protein Rv25112.550.01770
Rv2512chypothetical protein Rv2512c2.260.00053
Rv2513hypothetical protein Rv25130.870.00268
Rv2514chypothetical protein Rv2514c1.070.33966
Rv2515chypothetical protein Rv2515c4.260.03532
Rv2516chypothetical protein Rv2516c1.120.12309
Rv2517chypothetical protein Rv2517c1.890.00006
Rv2518clppS1.070.31373
Rv2519PE2.540.06324
Rv2520chypothetical protein Rv2520c1.700.01040
Rv2521bcp0.880.11628
Rv2522chypothetical protein Rv2522c0.630.00000
Rv2523cacpS1.440.00631
Rv2524cfas4.500.00737
Rv2525chypothetical protein Rv2525c4.150.02559
Rv2526hypothetical protein Rv25261.310.01486
Rv2527hypothetical protein Rv25273.040.02814
Rv2528cmrr0.850.19141
Rv2529hypothetical protein Rv25293.070.03856
Rv2530chypothetical protein Rv2530c1.160.15802
Rv2531cadi5.340.00500
Rv2532chypothetical protein Rv2532c0.900.14199
Rv2533cnusB4.010.22260
Rv2534cefp1.040.42008
Rv2536hypothetical protein Rv25361.070.38716
Rv2538caroB0.820.02476
Rv2539caroK1.110.48058
Rv2540caroF1.560.11445
Rv2541hypothetical protein Rv25410.140.37531
Rv2542hypothetical protein Rv25420.740.00185
Rv2543lppA2.460.05829
Rv2544lppB1.390.17298
Rv2545hypothetical protein Rv25451.950.05670
Rv2546hypothetical protein Rv25461.320.02845
Rv2547hypothetical protein Rv25471.730.01097
Rv2548hypothetical protein Rv25481.220.09934
Rv2549chypothetical protein Rv2549c1.340.13585
Rv2550chypothetical protein Rv2550c1.010.46317
Rv2551chypothetical protein Rv2551c0.700.00005
Rv2552caroE1.170.19946
Rv2553chypothetical protein Rv2553c1.740.06274
Rv2554chypothetical protein Rv2554c0.990.47562
Rv2555calaS0.960.33294
Rv2556chypothetical protein Rv2556c0.740.04046
Rv2557hypothetical protein Rv25571.160.20327
Rv2558hypothetical protein Rv25580.870.24229
Rv2559chypothetical protein Rv2559c1.150.07933
Rv2560hypothetical protein Rv25601.120.22725
Rv2561hypothetical protein Rv25613.380.02275
Rv2562hypothetical protein Rv25621.120.30580
Rv2563hypothetical protein Rv25632.520.01629
Rv2564glnQ0.890.11184
Rv2565hypothetical protein Rv25651.140.10470
Rv2566hypothetical protein Rv25662.390.00290
Rv2567hypothetical protein Rv25672.290.05235
Rv2568chypothetical protein Rv2568c1.860.04863
Rv2569chypothetical protein Rv2569c2.020.04431
Rv2570hypothetical protein Rv25701.300.05926
Rv2571chypothetical protein Rv2571c1.830.00807
Rv2572caspS0.910.37332
Rv2573hypothetical protein Rv25731.190.03423
Rv2574hypothetical protein Rv25741.000.49967
Rv2575hypothetical protein Rv25750.970.37682
Rv2576chypothetical protein Rv2576c0.880.23992
Rv2577hypothetical protein Rv25773.410.00799
Rv2578chypothetical protein Rv2578c1.660.06933
Rv2579linB2.140.01950
Rv2580chisS0.870.19858
Rv2581chypothetical protein Rv2581c0.950.27868
Rv2582ppiB0.960.42187
Rv2583crelA1.890.01739
Rv2584capt0.580.00007
Rv2585chypothetical protein Rv2585c2.280.07308
Rv2586csecF0.750.04265
Rv2588chypothetical protein Rv2588c0.990.49151
Rv2589gabT2.190.07390
Rv2590fadD90.790.04982
Rv2591PE_PGRS1.380.18442
Rv2592cruvB0.680.00136
Rv2593cruvA1.010.47946
Rv2594cruvC1.080.11605
Rv2595hypothetical protein Rv25951.010.44767
Rv2596hypothetical protein Rv25962.140.04759
Rv2597hypothetical protein Rv25970.850.00318
Rv2598hypothetical protein Rv25981.150.05166
Rv2599hypothetical protein Rv25991.710.01203
Rv2600hypothetical protein Rv26001.100.33636
Rv2601speE2.370.00130
Rv2602hypothetical protein Rv26020.820.05292
Rv2603chypothetical protein Rv2603c1.430.01820
Rv2604chypothetical protein Rv2604c0.640.00000
Rv2605ctesB20.970.39860
Rv2606chypothetical protein Rv2606c1.140.07462
Rv2607pdxH1.110.13425
Rv2608PPE1.500.08568
Rv2609chypothetical protein Rv2609c1.220.00444
Rv2610chypothetical protein Rv2610c1.070.37386
Rv2611chypothetical protein Rv2611c0.920.19731
Rv2612cpgsA0.760.04050
Rv2613chypothetical protein Rv2613c1.300.09674
Rv2614cthrS0.610.00174
Rv2615cPE_PGRS3.250.01623
Rv2616hypothetical protein Rv26160.710.00089
Rv2617chypothetical protein Rv2617c3.100.00068
Rv2618hypothetical protein Rv26180.940.15990
Rv2619chypothetical protein Rv2619c1.650.00041
Rv2620chypothetical protein Rv2620c0.710.03213
Rv2621chypothetical protein Rv2621c2.290.00882
Rv2622hypothetical protein Rv26221.470.01244
Rv2623hypothetical protein Rv262325.840.00024
Rv2624chypothetical protein Rv2624c2.290.04052
Rv2625chypothetical protein Rv2625c1.760.01152
Rv2626chypothetical protein Rv2626c14.580.00012
Rv2627chypothetical protein Rv2627c7.970.00799
Rv2628hypothetical protein Rv26288.360.00407
Rv2629hypothetical protein Rv26291.180.21118
Rv2630hypothetical protein Rv26302.340.02136
Rv2631hypothetical protein Rv26312.080.00128
Rv2632chypothetical protein Rv2632c0.740.01290
Rv2633chypothetical protein Rv2633c1.470.12951
Rv2634cPE_PGRS0.890.21565
Rv2635hypothetical protein Rv26350.890.04697
Rv2636hypothetical protein Rv26360.910.09629
Rv2637dedA0.700.00018
Rv2638hypothetical protein Rv26380.940.35381
Rv2639chypothetical protein Rv2639c1.000.45208
Rv2640chypothetical protein Rv2640c2.210.02820
Rv2641hypothetical protein Rv26411.430.00027
Rv2642hypothetical protein Rv26421.270.08078
Rv2643arsC3.460.00125
Rv2644chypothetical protein Rv2644c1.200.22872
Rv2645hypothetical protein Rv26451.370.00000
Rv2646hypothetical protein Rv26461.820.04165
Rv2647hypothetical protein Rv26470.930.08640
Rv2648hypothetical protein Rv26481.800.15314
Rv2649hypothetical protein Rv26492.710.05367
Rv2650chypothetical protein Rv2650c2.570.01063
Rv2651chypothetical protein Rv2651c1.900.00020
Rv2652chypothetical protein Rv2652c1.840.03276
Rv2653chypothetical protein Rv2653c2.050.02308
Rv2654chypothetical protein Rv2654c0.820.01710
Rv2655chypothetical protein Rv2655c1.740.00618
Rv2656chypothetical protein Rv2656c0.940.03462
Rv2657chypothetical protein Rv2657c2.800.00818
Rv2658chypothetical protein Rv2658c1.240.00571
Rv2659chypothetical protein Rv2659c3.280.00297
Rv2660chypothetical protein Rv2660c3.340.00552
Rv2661chypothetical protein Rv2661c1.320.00658
Rv2662hypothetical protein Rv26623.860.04735
Rv2663hypothetical protein Rv26632.340.01569
Rv2664hypothetical protein Rv26640.950.13426
Rv2665hypothetical protein Rv26651.070.23519
Rv2666hypothetical protein Rv26661.290.16892
Rv2667clpX′0.920.21150
Rv2668hypothetical protein Rv26680.930.37019
Rv2669hypothetical protein Rv26691.900.00877
Rv2670chypothetical protein Rv2670c0.860.08957
Rv2671ribD1.620.00021
Rv2672hypothetical protein Rv26721.410.09911
Rv2673hypothetical protein Rv26731.130.01703
Rv2674hypothetical protein Rv26741.220.23894
Rv2675chypothetical protein Rv2675c0.840.01330
Rv2676chypothetical protein Rv2676c1.290.12083
Rv2677chemY′0.790.02954
Rv2678chemE0.690.00322
Rv2679echA150.880.08346
Rv2680hypothetical protein Rv26800.700.00974
Rv2681hypothetical protein Rv26811.030.46070
Rv2682cdxs0.790.07711
Rv2683hypothetical protein Rv26830.900.17132
Rv2684arsA0.910.35179
Rv2685arsB1.150.17026
Rv2686chypothetical protein Rv2686c0.910.24549
Rv2687chypothetical protein Rv2687c0.770.02458
Rv2688chypothetical protein Rv2688c1.730.03285
Rv2689chypothetical protein Rv2689c1.620.07349
Rv2690chypothetical protein Rv2690c1.240.09009
Rv2691trkA1.120.12281
Rv2692trkB1.400.02287
Rv2693chypothetical protein Rv2693c0.870.11034
Rv2694chypothetical protein Rv2694c1.480.02250
Rv2695hypothetical protein Rv26951.310.00005
Rv2696chypothetical protein Rv2696c0.830.06041
Rv2697cdut1.190.00390
Rv2698hypothetical protein Rv26981.600.00039
Rv2699chypothetical protein Rv2699c1.570.00158
Rv2700hypothetical protein Rv27002.160.00881
Rv2701csuhB1.080.26093
Rv2702ppgK1.230.10523
Rv2703sigA0.910.18600
Rv2704hypothetical protein Rv27040.950.25417
Rv2705chypothetical protein Rv2705c1.970.04122
Rv2706chypothetical protein Rv2706c2.230.00106
Rv2707hypothetical protein Rv27073.100.00283
Rv2708chypothetical protein Rv2708c0.780.01348
Rv2709hypothetical protein Rv27091.400.05816
Rv2710sigB4.700.00001
Rv2711ideR1.780.00193
Rv2712chypothetical protein Rv2712c2.390.00592
Rv2713hypothetical protein Rv27132.670.00817
Rv2714hypothetical protein Rv27141.680.00788
Rv2715hypothetical protein Rv27151.530.05209
Rv2716hypothetical protein Rv27162.810.04132
Rv2717chypothetical protein Rv2717c1.660.05810
Rv2718chypothetical protein Rv2718c1.250.14938
Rv2719chypothetical protein Rv2719c1.660.06060
Rv2720lexA1.210.21206
Rv2721chypothetical protein Rv2721c1.370.16370
Rv2722hypothetical protein Rv27221.560.03751
Rv2723hypothetical protein Rv27232.040.03459
Rv2724cfadE200.820.02523
Rv2725chflX2.050.00697
Rv2726cdapF0.690.00000
Rv2727cmiaA2.980.04376
Rv2728chypothetical protein Rv2728c0.860.11802
Rv2729chypothetical protein Rv2729c2.450.01198
Rv2730hypothetical protein Rv27301.260.17416
Rv2731hypothetical protein Rv27310.800.00194
Rv2732chypothetical protein Rv2732c0.770.05528
Rv2733chypothetical protein Rv2733c1.200.19110
Rv2734hypothetical protein Rv27341.090.04926
Rv2735chypothetical protein Rv2735c3.820.05487
Rv2736crecX1.200.05063
Rv2737crecA1.220.08417
Rv2738chypothetical protein Rv2738c1.110.23716
Rv2739chypothetical protein Rv2739c1.520.02031
Rv2740hypothetical protein Rv27400.960.41676
Rv2741PE_PGRS1.460.00056
Rv2742chypothetical protein Rv2742c1.560.00000
Rv2743chypothetical protein Rv2743c1.430.00001
Rv2744c35kd_ag3.330.00012
Rv2745chypothetical protein Rv2745c1.120.22706
Rv2746cpgsA30.910.10923
Rv2747hypothetical protein Rv27471.170.07512
Rv2748cftsK0.930.31136
Rv2749hypothetical protein Rv27491.840.01074
Rv2750hypothetical protein Rv27501.540.04071
Rv2751hypothetical protein Rv27511.040.35378
Rv2752chypothetical protein Rv2752c0.900.15851
Rv2753cdapA1.360.10906
Rv2754chypothetical protein Rv2754c1.260.11460
Rv2755chsdS′2.780.02927
Rv2756chsdM1.280.10720
Rv2757chypothetical protein Rv2757c3.100.00981
Rv2758chypothetical protein Rv2758c1.000.49506
Rv2759chypothetical protein Rv2759c1.870.01170
Rv2760chypothetical protein Rv2760c1.100.22730
Rv2761chypothetical protein Rv2761c1.340.07453
Rv2762chypothetical protein Rv2762c0.930.13938
Rv2763cdfrA1.320.14250
Rv2764cthyA1.220.16879
Rv2765hypothetical protein Rv27653.190.01922
Rv2766cfabG51.160.23026
Rv2767chypothetical protein Rv2767c2.880.05569
Rv2768cPPE0.850.03167
Rv2769cPE3.060.04184
Rv2770cPPE1.800.12298
Rv2771chypothetical protein Rv2771c0.920.19053
Rv2772chypothetical protein Rv2772c0.690.00381
Rv2773cdapB0.970.41073
Rv2774chypothetical protein Rv2774c0.730.00199
Rv2775hypothetical protein Rv27751.020.45759
Rv2776chypothetical protein Rv2776c0.850.09592
Rv2777chypothetical protein Rv2777c2.470.02311
Rv2778chypothetical protein Rv2778c0.620.00029
Rv2779chypothetical protein Rv2779c1.560.11593
Rv2780ald2.430.00147
Rv2781chypothetical protein Rv2781c1.000.45641
Rv2782cpepR0.920.41579
Rv2783cgpsI0.580.00062
Rv2784clppU1.210.20832
Rv2785crpsO0.760.03661
Rv2786cribF1.080.22086
Rv2787hypothetical protein Rv27872.240.00825
Rv2788sirR1.020.44998
Rv2789cfadE211.250.02695
Rv2790cltp12.550.03996
Rv2791chypothetical protein Rv2791c1.450.00001
Rv2792chypothetical protein Rv2792c1.510.01051
Rv2793ctruB1.360.14355
Rv2794chypothetical protein Rv2794c0.980.46795
Rv2795chypothetical protein Rv2795c1.630.06238
Rv2796clppV1.040.40070
Rv2797chypothetical protein Rv2797c0.820.04301
Rv2798chypothetical protein Rv2798c0.620.00042
Rv2799hypothetical protein Rv27991.520.03443
Rv2800hypothetical protein Rv28001.080.16426
Rv2801chypothetical protein Rv2801c1.310.04550
Rv2802chypothetical protein Rv2802c0.970.41322
Rv2803chypothetical protein Rv2803c0.890.18622
Rv2804chypothetical protein Rv2804c1.510.02500
Rv2805hypothetical protein Rv28051.490.03138
Rv2806hypothetical protein Rv28061.180.20381
Rv2807hypothetical protein Rv28071.990.04213
Rv2808hypothetical protein Rv28081.700.14601
Rv2809hypothetical protein Rv28091.020.34900
Rv2810chypothetical protein Rv2810c2.260.01307
Rv2811hypothetical protein Rv28111.230.00000
Rv2812hypothetical protein Rv28120.850.07788
Rv2813hypothetical protein Rv28132.560.01615
Rv2814chypothetical protein Rv2814c1.850.12120
Rv2815chypothetical protein Rv2815c2.180.05931
Rv2816chypothetical protein Rv2816c0.620.00022
Rv2817chypothetical protein Rv2817c1.540.06478
Rv2818chypothetical protein Rv2818c0.800.08176
Rv2819chypothetical protein Rv2819c1.560.05724
Rv2820chypothetical protein Rv2820c2.350.06612
Rv2821chypothetical protein Rv2821c0.980.44563
Rv2822chypothetical protein Rv2822c0.990.47564
Rv2823chypothetical protein Rv2823c1.180.26622
Rv2824chypothetical protein Rv2824c0.730.01090
Rv2825chypothetical protein Rv2825c1.270.14124
Rv2826chypothetical protein Rv2826c1.430.13033
Rv2827chypothetical protein Rv2827c1.880.04334
Rv2828chypothetical protein Rv2828c1.160.23712
Rv2829chypothetical protein Rv2829c3.050.05554
Rv2830chypothetical protein Rv2830c0.710.00576
Rv2831echA161.380.19407
Rv2832cugpC0.940.15426
Rv2833cugpB3.790.00664
Rv2834cugpE2.150.03886
Rv2835cugpA2.420.00299
Rv2836cdinF0.810.13231
Rv2837chypothetical protein Rv2837c1.310.06924
Rv2838crbfA1.530.11850
Rv2839cinfB1.420.00025
Rv2840chypothetical protein Rv2840c0.570.00452
Rv2841cnusA1.550.00994
Rv2842chypothetical protein Rv2842c1.550.01074
Rv2843hypothetical protein Rv28432.170.00430
Rv2844hypothetical protein Rv28441.400.17267
Rv2845cproS1.890.00775
Rv2846cefpA0.540.00000
Rv2847ccysG21.140.19560
Rv2848ccobB1.080.28867
Rv2849ccobA0.850.15270
Rv2850chypothetical protein Rv2850c0.580.00103
Rv2851chypothetical protein Rv2851c1.550.06739
Rv2852chypothetical protein Rv2852c0.570.00007
Rv2853PE_PGRS0.980.45544
Rv2854hypothetical protein Rv28541.710.02749
Rv2855gorA1.330.19985
Rv2856nicT1.510.00958
Rv2857chypothetical protein Rv2857c1.300.09789
Rv2858caldC0.940.38765
Rv2859chypothetical protein Rv2859c0.960.40136
Rv2860cglnA41.310.16975
Rv2861cmap1.180.19417
Rv2862chypothetical protein Rv2862c0.610.00000
Rv2863hypothetical protein Rv28632.020.00791
Rv2864chypothetical protein Rv2864c0.760.00810
Rv2865hypothetical protein Rv28651.590.00505
Rv2866hypothetical protein Rv28660.610.00000
Rv2867chypothetical protein Rv2867c1.110.18368
Rv2868cgcpE0.710.00226
Rv2869chypothetical protein Rv2869c2.010.07171
Rv2870chypothetical protein Rv2870c1.370.12454
Rv2871hypothetical protein Rv28711.240.00339
Rv2872hypothetical protein Rv28721.610.06172
Rv2873mpt831.820.00939
Rv2874hypothetical protein Rv28740.780.01449
Rv2875mpt701.930.08411
Rv2876hypothetical protein Rv28760.940.27901
Rv2877chypothetical protein Rv2877c2.730.07693
Rv2878cmpt530.780.00399
Rv2879chypothetical protein Rv2879c0.610.00019
Rv2880chypothetical protein Rv2880c0.860.10168
Rv2881ccdsA0.900.15601
Rv2882cfrr0.900.27609
Rv2883cpyrH1.190.25302
Rv2884hypothetical protein Rv28841.100.18425
Rv2885chypothetical protein Rv2885c0.900.02155
Rv2886chypothetical protein Rv2886c1.190.28009
Rv2887hypothetical protein Rv28872.820.00877
Rv2888camiC0.950.30218
Rv2889ctsf1.070.32403
Rv2890crpsB0.980.43303
Rv2891hypothetical protein Rv28910.930.12329
Rv2892cPPE1.590.12745
Rv2893hypothetical protein Rv28932.300.01855
Rv2894cxerC1.590.07031
Rv2895cviuB0.840.07260
Rv2896chypothetical protein Rv2896c1.520.00767
Rv2897chypothetical protein Rv2897c1.320.01111
Rv2898chypothetical protein Rv2898c0.910.15658
Rv2899cfdhD1.690.01986
Rv2900cfdhF0.700.04645
Rv2901chypothetical protein Rv2901c0.780.06429
Rv2902crnhB0.730.03273
Rv2903clepB1.020.45146
Rv2904crplS0.790.05684
Rv2905lppW1.280.19870
Rv2906ctrmD0.660.00076
Rv2907crimM2.580.02823
Rv2908chypothetical protein Rv2908c0.880.25865
Rv2909crpsP0.970.37422
Rv2910chypothetical protein Rv2910c0.880.23535
Rv2911dacB1.840.01735
Rv2912chypothetical protein Rv2912c0.880.23840
Rv2913chypothetical protein Rv2913c1.530.00577
Rv2914cpknI1.960.00829
Rv2915chypothetical protein Rv2915c2.080.02931
Rv2916cffh1.130.23057
Rv2917hypothetical protein Rv29174.410.06488
Rv2918cglnD0.720.00736
Rv2919cglnB0.750.00082
Rv2920camt0.690.01380
Rv2921cftsY1.230.27260
Rv2922csmc0.730.13845
Rv2923chypothetical protein Rv2923c1.250.28096
Rv2924cfpg0.920.22400
Rv2925crnc1.190.23938
Rv2926chypothetical protein Rv2926c1.440.14914
Rv2927chypothetical protein Rv2927c1.020.45406
Rv2928tesA1.880.14310
Rv2929hypothetical protein Rv29291.710.13655
Rv2930fadD261.950.00748
Rv2931ppsA3.670.02082
Rv2932ppsB2.330.00823
Rv2933ppsC3.090.02651
Rv2934ppsD0.990.46482
Rv2935ppsE1.490.04733
Rv2936drrA0.960.29835
Rv2937drrB2.380.04492
Rv2938drrC1.340.11321
Rv2939papA50.950.29644
Rv2940cmas0.860.07588
Rv2941fadD281.670.01862
Rv2942mmpL71.280.19932
Rv2943hypothetical protein Rv29430.900.12105
Rv2944hypothetical protein Rv29442.090.02764
Rv2945clppX1.760.05843
Rv2946cpks10.940.38253
Rv2947cpks151.250.16500
Rv2948cfadD220.980.45365
Rv2949chypothetical protein Rv2949c1.020.46775
Rv2950cfadD291.210.24613
Rv2951chypothetical protein Rv2951c1.010.49143
Rv2952hypothetical protein Rv29520.790.01388
Rv2953hypothetical protein Rv29530.980.43151
Rv2954chypothetical protein Rv2954c0.770.08123
Rv2955chypothetical protein Rv2955c0.920.21665
Rv2956hypothetical protein Rv29560.690.00086
Rv2957hypothetical protein Rv29571.180.08778
Rv2958chypothetical protein Rv2958c4.230.03139
Rv2959chypothetical protein Rv2959c2.390.06953
Rv2960chypothetical protein Rv2960c1.510.14371
Rv2961hypothetical protein Rv29611.830.03294
Rv2962chypothetical protein Rv2962c4.250.02576
Rv2963hypothetical protein Rv29633.500.00842
Rv2964purU1.010.42498
Rv2965ckdtB2.680.08690
Rv2966chypothetical protein Rv2966c0.930.36524
Rv2967cpca1.630.15393
Rv2968chypothetical protein Rv2968c1.550.14923
Rv2969chypothetical protein Rv2969c0.910.32370
Rv2970clipN0.940.43046
Rv2971hypothetical protein Rv29712.690.07165
Rv2972chypothetical protein Rv2972c0.890.07015
Rv2973crecG2.200.10229
Rv2974chypothetical protein Rv2974c0.850.07257
Rv2975chypothetical protein Rv2975c1.280.20301
Rv2976cung0.870.11130
Rv2977cthiL0.830.12441
Rv2978chypothetical protein Rv2978c1.040.38454
Rv2979chypothetical protein Rv2979c2.850.00135
Rv2980hypothetical protein Rv29801.370.10657
Rv2981cddlA1.090.13693
Rv2982cgpdA20.980.45988
Rv2983hypothetical protein Rv29831.870.00436
Rv2984ppk1.160.28773
Rv2985mutT13.000.02448
Rv2986chupB0.440.00000
Rv2987cleuD1.090.35772
Rv2988cleuC0.750.03309
Rv2989hypothetical protein Rv29890.740.01923
Rv2990chypothetical protein Rv2990c1.130.28565
Rv2991hypothetical protein Rv29911.220.03607
Rv2992cgltS1.310.20774
Rv2993chypothetical protein Rv2993c1.140.21598
Rv2994hypothetical protein Rv29940.790.05130
Rv2995cleuB1.630.05390
Rv2996cserA0.970.40744
Rv2997hypothetical protein Rv29971.780.01605
Rv2998hypothetical protein Rv29980.740.03967
Rv2999lppY2.780.01537
Rv3000hypothetical protein Rv30001.090.27597
Rv3001cilvC0.860.09312
Rv3002cilvN0.530.00000
Rv3003cilvB0.910.28990
Rv3004hypothetical protein Rv30040.880.11012
Rv3005chypothetical protein Rv3005c2.120.06439
Rv3006lppZ0.720.00859
Rv3007chypothetical protein Rv3007c1.210.01678
Rv3008hypothetical protein Rv30081.480.09143
Rv3009cgatB3.750.01614
Rv3010cpfkA1.820.09395
Rv3011cgatA1.540.16158
Rv3012cgatC1.090.31638
Rv3013hypothetical protein Rv30131.820.12998
Rv3014cligA0.790.04331
Rv3015chypothetical protein Rv3015c0.660.00027
Rv3016lpqA1.860.01411
Rv3017chypothetical protein Rv3017c1.230.25383
Rv3018cPPE1.880.03773
Rv3019chypothetical protein Rv3019c1.560.05829
Rv3020cPE1.100.19035
Rv3021cPPE2.430.00313
Rv3022cPPE0.800.07372
Rv3023chypothetical protein Rv3023c3.280.04450
Rv3024chypothetical protein Rv3024c0.920.32289
Rv3025chypothetical protein Rv3025c0.890.00403
Rv3026chypothetical protein Rv3026c0.870.20926
Rv3027chypothetical protein Rv3027c1.100.28932
Rv3028cfixB0.660.01470
Rv3029cfixA1.230.18982
Rv3030hypothetical protein Rv30300.570.00001
Rv3031hypothetical protein Rv30311.190.23868
Rv3032hypothetical protein Rv30320.770.01324
Rv3033hypothetical protein Rv30331.210.01755
Rv3034chypothetical protein Rv3034c1.400.12390
Rv3035hypothetical protein Rv30351.140.11637
Rv3036chypothetical protein Rv3036c2.340.01882
Rv3037chypothetical protein Rv3037c1.230.06564
Rv3038chypothetical protein Rv3038c1.300.25881
Rv3039cechA171.330.01724
Rv3040chypothetical protein Rv3040c0.780.04927
Rv3041chypothetical protein Rv3041c1.870.04990
Rv3042cserB20.720.01309
Rv3043cctaD1.280.00482
Rv3044fecB0.690.00215
Rv3045adhC1.010.46223
Rv3046chypothetical protein Rv3046c0.820.08426
Rv3047chypothetical protein Rv3047c2.040.00430
Rv3048cnrdG2.900.00654
Rv3049chypothetical protein Rv3049c1.170.16164
Rv3050chypothetical protein Rv3050c1.170.29772
Rv3051cnrdE0.790.10716
Rv3052cnrdI0.970.36992
Rv3053cnrdH1.170.12744
Rv3054chypothetical protein Rv3054c1.230.02114
Rv3055hypothetical protein Rv30552.130.02698
Rv3056dinP2.310.04737
Rv3057chypothetical protein Rv3057c0.890.12510
Rv3058chypothetical protein Rv3058c1.640.03907
Rv3059hypothetical protein Rv30591.900.11130
Rv3060chypothetical protein Rv3060c0.640.00000
Rv3061cfadE221.120.26670
Rv3062ligB1.150.25663
Rv3063cstA0.660.00000
Rv3064chypothetical protein Rv3064c1.110.33230
Rv3065emrE0.730.00091
Rv3066hypothetical protein Rv30662.080.08153
Rv3067hypothetical protein Rv30671.830.12702
Rv3068cpgmA0.770.06616
Rv3069hypothetical protein Rv30692.290.07630
Rv3070hypothetical protein Rv30700.670.00004
Rv3071hypothetical protein Rv30711.240.08371
Rv3073chypothetical protein Rv3073c1.110.13040
Rv3074hypothetical protein Rv30742.920.00570
Rv3075chypothetical protein Rv3075c0.940.27367
Rv3076hypothetical protein Rv30763.960.00300
Rv3077atsF1.120.21159
Rv3078hypothetical protein Rv30781.690.00198
Rv3079chypothetical protein Rv3079c0.950.17004
Rv3080cpknK2.520.00015
Rv3081hypothetical protein Rv30810.890.05345
Rv3082cvirS3.390.00380
Rv3083hypothetical protein Rv30831.040.18967
Rv3084lipR1.310.00421
Rv3085hypothetical protein Rv30851.490.03231
Rv3086adhD0.890.13343
Rv3087hypothetical protein Rv30871.070.23857
Rv3088hypothetical protein Rv30882.140.00087
Rv3089fadD131.240.04200
Rv3090hypothetical protein Rv30901.930.02318
Rv3091hypothetical protein Rv30911.860.02699
Rv3092chypothetical protein Rv3092c1.190.13738
Rv3093chypothetical protein Rv3093c0.990.41246
Rv3094chypothetical protein Rv3094c1.070.33902
Rv3095hypothetical protein Rv30951.020.40385
Rv3096hypothetical protein Rv30961.220.22713
Rv3097cPE4.930.06727
Rv3098chypothetical protein Rv3098c1.170.17963
Rv3099chypothetical protein Rv3099c1.430.02539
Rv3100csmpB1.260.16847
Rv3101cftsX2.240.03022
Rv3102cftsE1.220.01337
Rv3103chypothetical protein Rv3103c1.150.00028
Rv3104chypothetical protein Rv3104c1.640.03335
Rv3105cprfB2.930.00136
Rv3106fprA1.220.14203
Rv3107chypothetical protein Rv3107c1.790.00389
Rv3108hypothetical protein Rv31081.620.02983
Rv3109moaA0.810.00032
Rv3110moaB3.370.05826
Rv3111moaC0.960.42261
Rv3112moaD1.230.27165
Rv3113hypothetical protein Rv31132.070.10127
Rv3114hypothetical protein Rv31141.410.10431
Rv3115hypothetical protein Rv31153.320.04543
Rv3116moeB0.830.17244
Rv3117cysA30.850.22256
Rv3118sseC0.510.00011
Rv3119moaE1.080.40147
Rv3120hypothetical protein Rv31202.360.09363
Rv3121hypothetical protein Rv31212.210.03634
Rv3122hypothetical protein Rv31220.870.00090
Rv3123hypothetical protein Rv31231.880.05108
Rv3124hypothetical protein Rv31241.920.01620
Rv3125cPPE1.600.01793
Rv3126chypothetical protein Rv3126c2.160.00012
Rv3127hypothetical protein Rv31279.490.02020
Rv3128chypothetical protein Rv3128c3.180.00420
Rv3129hypothetical protein Rv31292.120.04078
Rv3130chypothetical protein Rv3130c15.360.00037
Rv3131hypothetical protein Rv313111.360.00080
Rv3132chypothetical protein Rv3132c4.830.00134
Rv3133chypothetical protein Rv3133c2.130.00748
Rv3134chypothetical protein Rv3134c7.060.00688
Rv3135PPE1.490.06951
Rv3136PPE0.860.12183
Rv3137hypothetical protein Rv31372.090.01570
Rv3138pflA1.930.00899
Rv3139fadE245.800.00421
Rv3140fadE233.540.01414
Rv3141fadB41.090.17330
Rv3142chypothetical protein Rv3142c1.130.20360
Rv3143hypothetical protein Rv31431.880.02475
Rv3144cPPE1.050.19138
Rv3145nuoA1.130.31117
Rv3146nuoB0.530.00273
Rv3147nuoC1.620.13189
Rv3148nuoD0.450.00003
Rv3149nuoE1.430.11004
Rv3150nuoF0.780.21139
Rv3151nuoG1.090.32037
Rv3152nuoH1.790.09022
Rv3153nuoI0.880.28299
Rv3154nuoJ0.620.00001
Rv3155nuoK1.180.26070
Rv3156nuoL0.660.00191
Rv3157nuoM2.230.13427
Rv3158nuoN0.510.00000
Rv3159cPPE0.910.15166
Rv3160chypothetical protein Rv3160c1.120.30677
Rv3161chypothetical protein Rv3161c1.950.05539
Rv3162chypothetical protein Rv3162c0.770.06955
Rv3163chypothetical protein Rv3163c2.420.03986
Rv3164cmoxR31.300.01173
Rv3165chypothetical protein Rv3165c3.240.06883
Rv3166chypothetical protein Rv3166c1.080.00332
Rv3167chypothetical protein Rv3167c1.080.34129
Rv3168hypothetical protein Rv31680.710.00000
Rv3169hypothetical protein Rv31691.020.37200
Rv3170hypothetical protein Rv31701.790.04150
Rv3171chpx0.850.03078
Rv3172chypothetical protein Rv3172c1.670.07897
Rv3173chypothetical protein Rv3173c1.850.04692
Rv3174hypothetical protein Rv31741.180.23323
Rv3175hypothetical protein Rv31751.700.00516
Rv3176clipS1.510.11063
Rv3177hypothetical protein Rv31771.730.04845
Rv3178hypothetical protein Rv31783.450.02179
Rv3179hypothetical protein Rv31791.250.01069
Rv3180chypothetical protein Rv3180c3.290.01208
Rv3181chypothetical protein Rv3181c1.530.00068
Rv3182hypothetical protein Rv31822.740.06192
Rv3183hypothetical protein Rv31831.620.04730
Rv3184hypothetical protein Rv31841.360.24449
Rv3185hypothetical protein Rv31852.780.05989
Rv3186hypothetical protein Rv31861.380.20911
Rv3187hypothetical protein Rv31872.640.05540
Rv3188hypothetical protein Rv31881.490.00984
Rv3189hypothetical protein Rv31892.180.00400
Rv3190chypothetical protein Rv3190c1.130.30350
Rv3191chypothetical protein Rv3191c4.750.02392
Rv3192hypothetical protein Rv31921.470.01042
Rv3193chypothetical protein Rv3193c1.400.05735
Rv3194chypothetical protein Rv3194c1.580.09728
Rv3195hypothetical protein Rv31952.940.03687
Rv3196hypothetical protein Rv31960.790.05091
Rv3197hypothetical protein Rv31971.510.05102
Rv3198cuvrD20.810.07929
Rv3199chypothetical protein Rv3199c1.720.04179
Rv3200chypothetical protein Rv3200c0.810.10355
Rv3201chypothetical protein Rv3201c1.050.28054
Rv3202chypothetical protein Rv3202c0.670.01692
Rv3203lipV1.290.01124
Rv3204hypothetical protein Rv32041.640.09539
Rv3205chypothetical protein Rv3205c0.990.46895
Rv3206cmoeZ0.980.44412
Rv3207chypothetical protein Rv3207c4.000.08772
Rv3208hypothetical protein Rv32080.950.36123
Rv3209hypothetical protein Rv32090.990.47169
Rv3210chypothetical protein Rv3210c1.040.43912
Rv3211rhlE0.700.04024
Rv3212hypothetical protein Rv32120.680.03008
Rv3213chypothetical protein Rv3213c1.490.19765
Rv3214entD0.890.20269
Rv3215entC1.570.13877
Rv3216hypothetical protein Rv32160.740.00472
Rv3217chypothetical protein Rv3217c1.070.28830
Rv3218hypothetical protein Rv32181.520.01068
Rv3219whiB10.910.02113
Rv3220chypothetical protein Rv3220c0.650.00339
Rv3221chypothetical protein Rv3221c0.820.01296
Rv3222chypothetical protein Rv3222c1.540.00029
Rv3223csigH3.410.00429
Rv3224hypothetical protein Rv32241.150.21521
Rv3225chypothetical protein Rv3225c1.640.04300
Rv3226chypothetical protein Rv3226c1.430.00087
Rv3227aroA2.780.02827
Rv3228hypothetical protein Rv32281.790.06263
Rv3229cdesA31.280.18746
Rv3230chypothetical protein Rv3230c1.900.04881
Rv3231chypothetical protein Rv3231c3.600.01542
Rv3232cpvdS0.710.01664
Rv3233chypothetical protein Rv3233c1.270.08863
Rv3234chypothetical protein Rv3234c1.130.37662
Rv3235hypothetical protein Rv32354.080.02502
Rv3236ckefB1.040.39997
Rv3237chypothetical protein Rv3237c0.900.11136
Rv3238chypothetical protein Rv3238c0.610.00087
Rv3239chypothetical protein Rv3239c1.210.13929
Rv3240csecA0.830.09786
Rv3241chypothetical protein Rv3241c1.790.01956
Rv3242chypothetical protein Rv3242c1.200.19643
Rv3243chypothetical protein Rv3243c1.260.05160
Rv3244clpqB1.050.39932
Rv3245cmtrB3.560.03339
Rv3246cmtrA1.110.30173
Rv3247ctmk1.250.04148
Rv3248csahH1.070.37392
Rv3249chypothetical protein Rv3249c1.550.10356
Rv3250crubB0.880.00721
Rv3251crubA1.480.07865
Rv3252chypothetical protein Rv3252c2.780.06568
Rv3253chypothetical protein Rv3253c0.950.38700
Rv3254hypothetical protein Rv32540.880.25035
Rv3255cmanA1.110.31437
Rv3256chypothetical protein Rv3256c0.760.07567
Rv3257cpmmA0.860.00053
Rv3258chypothetical protein Rv3258c0.690.00884
Rv3259hypothetical protein Rv32591.040.42999
Rv3260cwhiB20.620.00049
Rv3261hypothetical protein Rv32611.950.14297
Rv3262hypothetical protein Rv32620.830.03386
Rv3263hypothetical protein Rv32631.510.07391
Rv3264crmlA20.640.01531
Rv3265cwbbL2.120.02831
Rv3266crmlD1.730.03624
Rv3267hypothetical protein Rv32671.930.07271
Rv3268hypothetical protein Rv32682.060.01737
Rv3269hypothetical protein Rv32691.270.11489
Rv3270ctpC2.350.00081
Rv3271chypothetical protein Rv3271c1.470.04208
Rv3272hypothetical protein Rv32721.720.08938
Rv3273hypothetical protein Rv32731.180.29007
Rv3274cfadE251.160.09228
Rv3275cpurE1.400.04458
Rv3276cpurK2.110.04597
Rv3277hypothetical protein Rv32771.380.02454
Rv3278chypothetical protein Rv3278c0.940.40517
Rv3279cbirA3.070.04098
Rv3280accD51.950.07278
Rv3281hypothetical protein Rv32810.570.00192
Rv3282hypothetical protein Rv32822.230.02989
Rv3283sseA0.920.23093
Rv3284hypothetical protein Rv32841.150.18177
Rv3285accA31.250.14719
Rv3286csigF1.020.45768
Rv3287crsbW4.580.00051
Rv3288chypothetical protein Rv3288c1.830.01907
Rv3289chypothetical protein Rv3289c1.940.03139
Rv3290clat5.590.00864
Rv3291chypothetical protein Rv3291c1.700.00502
Rv3292hypothetical protein Rv32921.570.00251
Rv3293aldB1.170.00186
Rv3294hypothetical protein Rv32940.700.00000
Rv3295hypothetical protein Rv32950.970.45574
Rv3296lhr1.740.00218
Rv3297nei2.540.01338
Rv3298clpqC1.040.37722
Rv3299catsB1.060.33981
Rv3300chypothetical protein Rv3300c1.520.16456
Rv3301cphoY11.820.14106
Rv3302cglpD20.820.16515
Rv3303clpdA2.340.08355
Rv3304hypothetical protein Rv33040.710.00048
Rv3305camiA3.600.08888
Rv3306camiB0.530.00000
Rv3307deoD1.620.20954
Rv3308pmmB0.450.00000
Rv3309cupp0.900.31359
Rv3310hypothetical protein Rv33101.560.15190
Rv3311hypothetical protein Rv33111.120.33212
Rv3312chypothetical protein Rv3312c0.770.06475
Rv3313cadd1.700.00390
Rv3314cdeoA1.730.03139
Rv3315ccdd0.820.08961
Rv3316sdhC1.250.17277
Rv3317sdhD0.720.00012
Rv3318sdhA1.540.01649
Rv3319sdhB1.580.04031
Rv3320chypothetical protein Rv3320c2.920.01743
Rv3321chypothetical protein Rv3321c0.910.27034
Rv3322cmoaE34.890.02068
Rv3323cgphA1.400.15141
Rv3324cmoaC31.850.01741
Rv3325hypothetical protein Rv33251.950.09245
Rv3326hypothetical protein Rv33262.070.02164
Rv3327hypothetical protein Rv33270.790.01080
Rv3328csigJ1.210.27673
Rv3329hypothetical protein Rv33291.320.13551
Rv3330hypothetical protein Rv33302.380.04316
Rv3331sugI1.660.01716
Rv3332nagA1.770.00217
Rv3333chypothetical protein Rv3333c4.950.01583
Rv3334hypothetical protein Rv33343.310.00023
Rv3335chypothetical protein Rv3335c3.010.00970
Rv3336ctrpS0.800.00518
Rv3337hypothetical protein Rv33371.520.00128
Rv3338hypothetical protein Rv33380.870.22290
Rv3339cicd10.840.02092
Rv3340metC2.770.00067
Rv3341metA1.140.23739
Rv3342hypothetical protein Rv33421.750.08017
Rv3343cPPE8.090.05291
Rv3344cPE_PGRS2.160.14594
Rv3345cPE_PGRS5.180.09996
Rv3346chypothetical protein Rv3346c1.150.25223
Rv3347cPPE13.090.01320
Rv3348hypothetical protein Rv33481.060.37443
Rv3349chypothetical protein Rv3349c13.960.03784
Rv3350cPPE1.530.11423
Rv3351chypothetical protein Rv3351c2.950.08169
Rv3352chypothetical protein Rv3352c0.960.32326
Rv3353chypothetical protein Rv3353c1.740.05641
Rv3354hypothetical protein Rv33540.980.43085
Rv3355chypothetical protein Rv3355c2.830.09095
Rv3356cfolD1.630.11369
Rv3357hypothetical protein Rv33571.800.11759
Rv3358hypothetical protein Rv33581.540.08361
Rv3359hypothetical protein Rv33591.360.17407
Rv3360hypothetical protein Rv33601.300.05231
Rv3361chypothetical protein Rv3361c1.400.12656
Rv3362chypothetical protein Rv3362c0.950.25962
Rv3363chypothetical protein Rv3363c2.080.05007
Rv3364chypothetical protein Rv3364c0.780.03620
Rv3365chypothetical protein Rv3365c1.540.13777
Rv3366spoU0.740.00015
Rv3367PE_PGRS1.020.30467
Rv3368chypothetical protein Rv3368c1.200.25124
Rv3369hypothetical protein Rv33690.940.23774
Rv3370cdnaE23.560.01869
Rv3371hypothetical protein Rv33711.890.02063
Rv3372otsB21.110.34111
Rv3373echA182.020.03648
Rv3374echA18′0.970.44299
Rv3375amiD1.730.00352
Rv3376hypothetical protein Rv33760.630.00262
Rv3377chypothetical protein Rv3377c2.290.01137
Rv3378chypothetical protein Rv3378c0.970.45047
Rv3379chypothetical protein Rv3379c1.440.02013
Rv3380chypothetical protein Rv3380c1.860.10723
Rv3381chypothetical protein Rv3381c1.510.14524
Rv3382clytB0.830.02359
Rv3383cidsB1.200.09936
Rv3384chypothetical protein Rv3384c2.240.05665
Rv3385chypothetical protein Rv3385c1.420.00014
Rv3386hypothetical protein Rv33860.760.11586
Rv3387hypothetical protein Rv33872.690.03112
Rv3388PE_PGRS0.840.07700
Rv3389chypothetical protein Rv3389c1.910.04137
Rv3390lpqD1.380.20881
Rv3391acrA11.910.06617
Rv3392ccmaA11.510.12682
Rv3393iunH1.390.08824
Rv3394chypothetical protein Rv3394c0.820.09902
Rv3395chypothetical protein Rv3395c1.390.05278
Rv3396cguaA1.460.05905
Rv3397cphyA1.380.07370
Rv3398cidsA5.250.13070
Rv3399hypothetical protein Rv33991.100.20706
Rv3401hypothetical protein Rv34011.900.18283
Rv3402chypothetical protein Rv3402c1.860.29686
Rv3403chypothetical protein Rv3403c2.500.05817
Rv3404chypothetical protein Rv3404c0.000.14325
Rv3405chypothetical protein Rv3405c1.180.30598
Rv3406hypothetical protein Rv34062.570.15256
Rv3407hypothetical protein Rv34070.560.01913
Rv3408hypothetical protein Rv34080.700.03316
Rv3409cchoD1.030.41306
Rv3410cguaB31.410.12393
Rv3411cguaB21.160.10913
Rv3412hypothetical protein Rv34121.260.11522
Rv3413chypothetical protein Rv3413c1.640.09733
Rv3414csigD0.880.03625
Rv3415chypothetical protein Rv3415c3.750.02823
Rv3416whiB31.100.25742
Rv3417cgroEL11.150.21246
Rv3418cgroES1.680.00423
Rv3419cgcp0.990.43361
Rv3420crimI1.430.10245
Rv3421chypothetical protein Rv3421c1.100.20211
Rv3422chypothetical protein Rv3422c2.670.08006
Rv3423calr1.080.25623
Rv3424chypothetical protein Rv3424c1.430.06883
Rv3425PPE1.450.09891
Rv3426PPE1.700.11033
Rv3427chypothetical protein Rv3427c1.260.00633
Rv3428chypothetical protein Rv3428c1.670.01236
Rv3429PPE1.800.03086
Rv3430chypothetical protein Rv3430c1.950.03363
Rv3431chypothetical protein Rv3431c1.110.05691
Rv3432cgadB0.830.08441
Rv3433chypothetical protein Rv3433c1.900.03983
Rv3434chypothetical protein Rv3434c0.770.00000
Rv3435chypothetical protein Rv3435c1.600.16144
Rv3436cglmS0.800.01351
Rv3437hypothetical protein Rv34370.970.42478
Rv3438hypothetical protein Rv34382.530.05972
Rv3439chypothetical protein Rv3439c0.990.41805
Rv3440chypothetical protein Rv3440c0.650.00038
Rv3441cmrsA1.320.22656
Rv3442crpsI0.660.01022
Rv3443crplM0.840.20824
Rv3444chypothetical protein Rv3444c0.790.00019
Rv3445chypothetical protein Rv3445c1.310.26960
Rv3446chypothetical protein Rv3446c1.170.19650
Rv3447chypothetical protein Rv3447c1.260.15794
Rv3448hypothetical protein Rv34481.650.09235
Rv3449hypothetical protein Rv34492.470.08011
Rv3450chypothetical protein Rv3450c0.710.00004
Rv3451hypothetical protein Rv34512.430.04513
Rv3452hypothetical protein Rv34520.780.03341
Rv3453hypothetical protein Rv34532.380.05151
Rv3454hypothetical protein Rv34541.390.07555
Rv3455ctruA1.450.08907
Rv3456crplQ0.430.00000
Rv3457crpoA0.670.00000
Rv3458crpsD0.650.00082
Rv3459crpsK0.710.00019
Rv3460crpsM0.950.21113
Rv3461crpmJ0.560.00000
Rv3462cinfA0.510.00003
Rv3463hypothetical protein Rv34631.580.01285
Rv3464rmlB2.770.03653
Rv3465rmlC0.910.28413
Rv3466hypothetical protein Rv34661.490.00003
Rv3467hypothetical protein Rv34671.580.06779
Rv3468crmlB33.220.00320
Rv3469cmhpE3.290.03261
Rv3470cilvB22.840.03006
Rv3471chypothetical protein Rv3471c3.210.01319
Rv3472hypothetical protein Rv34720.920.28306
Rv3473cbpoA1.670.00568
Rv3474hypothetical protein Rv34741.390.09240
Rv3475hypothetical protein Rv34753.490.04337
Rv3476ckgtP2.010.05901
Rv3477PE0.660.07147
Rv3478PPE0.580.00266
Rv3479hypothetical protein Rv34791.310.14416
Rv3480chypothetical protein Rv3480c1.090.25646
Rv3481chypothetical protein Rv3481c1.390.11569
Rv3482chypothetical protein Rv3482c0.630.00014
Rv3483chypothetical protein Rv3483c0.980.40316
Rv3484cpsA0.750.00362
Rv3485chypothetical protein Rv3485c1.400.04493
Rv3486hypothetical protein Rv34860.680.00007
Rv3487clipF0.960.44290
Rv3488hypothetical protein Rv34881.180.30826
Rv3489hypothetical protein Rv34890.770.02537
Rv3490otsA1.850.06368
Rv3491hypothetical protein Rv34911.280.17228
Rv3492chypothetical protein Rv3492c0.740.02637
Rv3493chypothetical protein Rv3493c1.530.18149
Rv3494chypothetical protein Rv3494c0.780.08215
Rv3495clprN1.990.12899
Rv3496chypothetical protein Rv3496c3.120.08886
Rv3497chypothetical protein Rv3497c2.470.05149
Rv3498chypothetical protein Rv3498c0.980.43550
Rv3499cmce41.240.25204
Rv3500chypothetical protein Rv3500c1.270.25918
Rv3501chypothetical protein Rv3501c1.370.21523
Rv3502chypothetical protein Rv3502c0.880.06191
Rv3503cfdxD1.870.04600
Rv3504fadE261.630.00109
Rv3505fadE271.320.05415
Rv3506fadD171.450.02500
Rv3507PE_PGRS1.070.37104
Rv3508PE_PGRS0.970.39733
Rv3509cilvX0.910.08815
Rv3510chypothetical protein Rv3510c1.190.17412
Rv3511PE_PGRS1.180.01052
Rv3512PE_PGRS1.150.00312
Rv3513cfadD181.680.01578
Rv3514PE_PGRS0.990.47621
Rv3515cfadD193.120.00910
Rv3516echA191.490.06493
Rv3517hypothetical protein Rv35171.140.01839
Rv3518chypothetical protein Rv3518c1.410.02979
Rv3519hypothetical protein Rv35191.150.16900
Rv3520chypothetical protein Rv3520c0.950.32820
Rv3521hypothetical protein Rv35212.880.00874
Rv3522hypothetical protein Rv35221.170.11270
Rv3523hypothetical protein Rv35231.230.11106
Rv3524hypothetical protein Rv35241.710.02327
Rv3525chypothetical protein Rv3525c1.250.05067
Rv3526hypothetical protein Rv35261.240.00376
Rv3527hypothetical protein Rv35272.480.05851
Rv3528chypothetical protein Rv3528c1.090.31711
Rv3529chypothetical protein Rv3529c1.520.00916
Rv3530chypothetical protein Rv3530c2.270.02883
Rv3531chypothetical protein Rv3531c2.390.00294
Rv3532PPE2.940.01497
Rv3533cPPE1.570.08755
Rv3534chypothetical protein Rv3534c1.540.00177
Rv3535chypothetical protein Rv3535c1.300.01288
Rv3536chypothetical protein Rv3536c0.760.02299
Rv3537hypothetical protein Rv35371.750.00060
Rv3538ufaA22.080.00280
Rv3539PE7.610.00599
Rv3540cltp21.050.40774
Rv3541chypothetical protein Rv3541c1.190.19158
Rv3542chypothetical protein Rv3542c2.260.04832
Rv3543cfadE292.400.06398
Rv3544cfadE282.630.06079
Rv3545chypothetical protein Rv3545c1.120.05221
Rv3546fadA51.490.16829
Rv3547hypothetical protein Rv35471.110.20915
Rv3548chypothetical protein Rv3548c0.850.01333
Rv3549chypothetical protein Rv3549c2.350.03833
Rv3550echA200.940.27249
Rv3551hypothetical protein Rv35512.100.08295
Rv3552hypothetical protein Rv35521.180.27238
Rv3553hypothetical protein Rv35531.390.06305
Rv3554fdxB0.930.28609
Rv3555chypothetical protein Rv3555c1.530.04160
Rv3556cfadA60.920.29713
Rv3557chypothetical protein Rv3557c1.860.01990
Rv3558PPE1.610.07843
Rv3559chypothetical protein Rv3559c1.630.02191
Rv3560cfadE302.070.05771
Rv3561fadD31.680.08003
Rv3562fadE311.060.20402
Rv3563fadE321.170.09331
Rv3564fadE331.400.07759
Rv3565aspB1.020.43176
Rv3566cnhoA1.160.33618
Rv3567chypothetical protein Rv3567c2.220.04362
Rv3568chypothetical protein Rv3568c1.330.14612
Rv3569chypothetical protein Rv3569c0.910.24673
Rv3570chypothetical protein Rv3570c0.690.00009
Rv3571hypothetical protein Rv35712.190.01085
Rv3572hypothetical protein Rv35722.840.04517
Rv3573cfadE341.450.06980
Rv3574hypothetical protein Rv35741.030.39857
Rv3575chypothetical protein Rv3575c3.190.01175
Rv3576pknM0.660.00027
Rv3577hypothetical protein Rv35771.480.09430
Rv3578arsB20.710.00056
Rv3579chypothetical protein Rv3579c0.950.37384
Rv3580ccysS1.170.10740
Rv3581chypothetical protein Rv3581c1.030.37106
Rv3582chypothetical protein Rv3582c1.180.16538
Rv3583chypothetical protein Rv3583c0.610.00001
Rv3584lpqE0.420.00000
Rv3585radA1.050.24700
Rv3586hypothetical protein Rv35860.910.20970
Rv3587chypothetical protein Rv3587c2.200.02322
Rv3588chypothetical protein Rv3588c0.810.03836
Rv3589mutY1.750.11301
Rv3590cPE_PGRS3.590.15730
Rv3591chypothetical protein Rv3591c1.790.05653
Rv3592hypothetical protein Rv35920.520.00000
Rv3593lpqF0.630.00000
Rv3594hypothetical protein Rv35940.750.00221
Rv3595cPE_PGRS1.280.13962
Rv3596cclpC0.660.01833
Rv3597clsr21.310.11830
Rv3598clysS1.160.23713
Rv3599chypothetical protein Rv3599c3.260.07093
Rv3600chypothetical protein Rv3600c1.010.45779
Rv3601cpanD1.770.00105
Rv3602cpanC1.720.02963
Rv3603chypothetical protein Rv3603c1.760.00002
Rv3604chypothetical protein Rv3604c1.890.00952
Rv3605chypothetical protein Rv3605c0.960.35437
Rv3606cfolK1.050.40125
Rv3607cfolX1.080.14688
Rv3608cfolP1.390.03600
Rv3609cfolE0.920.31578
Rv3610cftsH1.400.02413
Rv3611hypothetical protein Rv36111.590.02714
Rv3612chypothetical protein Rv3612c0.760.09531
Rv3613chypothetical protein Rv3613c1.830.01818
Rv3614chypothetical protein Rv3614c0.990.48969
Rv3615chypothetical protein Rv3615c2.020.15176
Rv3616chypothetical protein Rv3616c0.940.45148
Rv3617ephA1.680.00189
Rv3618hypothetical protein Rv36180.510.00061
Rv3619chypothetical protein Rv3619c0.480.00000
Rv3620chypothetical protein Rv3620c0.300.00000
Rv3621cPPE1.060.40476
Rv3622cPE1.870.06139
Rv3623lpqG1.320.05084
Rv3624chpt0.620.00074
Rv3625cmesJ1.100.22637
Rv3626chypothetical protein Rv3626c1.320.15538
Rv3627chypothetical protein Rv3627c1.680.00193
Rv3628ppa0.890.27729
Rv3629chypothetical protein Rv3629c1.210.16293
Rv3630hypothetical protein Rv36300.830.15263
Rv3631hypothetical protein Rv36310.900.13164
Rv3632hypothetical protein Rv36321.190.32258
Rv3633hypothetical protein Rv36331.070.32767
Rv3634crmlB20.810.00737
Rv3635hypothetical protein Rv36351.480.04311
Rv3636hypothetical protein Rv36360.650.00014
Rv3637hypothetical protein Rv36373.610.04899
Rv3638hypothetical protein Rv36381.180.23884
Rv3639chypothetical protein Rv3639c5.060.01837
Rv3640chypothetical protein Rv3640c0.690.00002
Rv3641cfic1.090.35147
Rv3642chypothetical protein Rv3642c1.070.35853
Rv3643hypothetical protein Rv36433.580.03715
Rv3644chypothetical protein Rv3644c1.530.14032
Rv3645hypothetical protein Rv36450.930.25380
Rv3646ctopA1.350.19349
Rv3647chypothetical protein Rv3647c0.820.05926
Rv3648ccspA0.130.00000
Rv3649hypothetical protein Rv36491.730.02063
Rv3650PE4.610.00783
Rv3651hypothetical protein Rv36513.170.00384
Rv3652PE_PGRS1.740.00051
Rv3653PE_PGRS1.220.00002
Rv3654chypothetical protein Rv3654c1.070.27791
Rv3655chypothetical protein Rv3655c3.640.03178
Rv3656chypothetical protein Rv3656c1.060.26561
Rv3657chypothetical protein Rv3657c1.120.08130
Rv3658chypothetical protein Rv3658c1.380.05158
Rv3659ctrbB1.000.49922
Rv3660chypothetical protein Rv3660c2.030.00035
Rv3661hypothetical protein Rv36612.320.02382
Rv3662chypothetical protein Rv3662c1.420.00002
Rv3663cdppD2.950.05715
Rv3664cdppC1.490.04136
Rv3665cdppB3.390.01285
Rv3666cdppA0.890.07753
Rv3667acs2.020.02356
Rv3668chypothetical protein Rv3668c1.710.00422
Rv3669hypothetical protein Rv36693.040.00450
Rv3670ephE2.720.06882
Rv3671chypothetical protein Rv3671c1.900.04611
Rv3672chypothetical protein Rv3672c1.030.39211
Rv3673chypothetical protein Rv3673c1.510.09442
Rv3674cnth1.120.21819
Rv3675hypothetical protein Rv36751.310.04044
Rv3676hypothetical protein Rv36761.180.29455
Rv3677chypothetical protein Rv3677c0.720.00374
Rv3678chypothetical protein Rv3678c0.570.00044
Rv3679hypothetical protein Rv36791.500.10581
Rv3680hypothetical protein Rv36800.740.03538
Rv3681cwhiB41.920.02947
Rv3682ponA′1.210.33829
Rv3683hypothetical protein Rv36831.350.05818
Rv3684hypothetical protein Rv36840.870.20142
Rv3685chypothetical protein Rv3685c0.860.05680
Rv3686chypothetical protein Rv3686c0.560.01248
Rv3687chypothetical protein Rv3687c1.760.14789
Rv3688chypothetical protein Rv3688c0.530.00000
Rv3689hypothetical protein Rv36892.740.07155
Rv3690hypothetical protein Rv36900.870.21707
Rv3691hypothetical protein Rv36911.030.43509
Rv3692moxR21.070.40076
Rv3693hypothetical protein Rv36930.960.43273
Rv3694chypothetical protein Rv3694c0.760.00513
Rv3695hypothetical protein Rv36952.190.09410
Rv3696cglpK0.700.00927
Rv3697chypothetical protein Rv3697c3.270.04006
Rv3698hypothetical protein Rv36982.620.00835
Rv3699hypothetical protein Rv36991.850.01924
Rv3700chypothetical protein Rv3700c0.860.11903
Rv3701chypothetical protein Rv3701c1.110.25300
Rv3702chypothetical protein Rv3702c2.310.01081
Rv3703chypothetical protein Rv3703c0.890.06791
Rv3704cgshA1.670.00713
Rv3705chypothetical protein Rv3705c1.200.20596
Rv3706chypothetical protein Rv3706c6.860.01995
Rv3707chypothetical protein Rv3707c1.290.07234
Rv3708casd1.460.10380
Rv3709cask1.420.04555
Rv3710leuA1.230.15218
Rv3711cdnaQ0.950.37342
Rv3712hypothetical protein Rv37121.550.01620
Rv3713cobQ21.710.06423
Rv3714chypothetical protein Rv3714c1.300.00000
Rv3715crecR1.050.30667
Rv3716chypothetical protein Rv3716c1.250.10267
Rv3717hypothetical protein Rv37171.350.11525
Rv3718chypothetical protein Rv3718c0.910.27036
Rv3719hypothetical protein Rv37191.110.27339
Rv3720hypothetical protein Rv37201.400.08770
Rv3721cdnaZX3.140.02265
Rv3722chypothetical protein Rv3722c0.620.00015
Rv3723hypothetical protein Rv37231.470.02267
Rv3724hypothetical protein Rv37241.230.18655
Rv3725hypothetical protein Rv37250.980.38003
Rv3726hypothetical protein Rv37260.730.00451
Rv3727hypothetical protein Rv37271.090.30819
Rv3728hypothetical protein Rv37281.160.23619
Rv3729hypothetical protein Rv37291.600.00009
Rv3730chypothetical protein Rv3730c1.100.11314
Rv3731ligC1.970.03330
Rv3732hypothetical protein Rv37320.960.38275
Rv3733chypothetical protein Rv3733c2.890.09991
Rv3734chypothetical protein Rv3734c0.570.00009
Rv3735hypothetical protein Rv37351.380.20893
Rv3736hypothetical protein Rv37360.980.45520
Rv3737hypothetical protein Rv37374.660.06929
Rv3738cPPE2.510.10593
Rv3739cPPE1.830.14086
Rv3740chypothetical protein Rv3740c1.180.31662
Rv3741chypothetical protein Rv3741c1.840.05691
Rv3742chypothetical protein Rv3742c0.830.07679
Rv3743chypothetical protein Rv3743c1.350.18649
Rv3744hypothetical protein Rv37441.100.33378
Rv3745chypothetical protein Rv3745c1.940.02418
Rv3746cPE2.030.06670
Rv3747hypothetical protein Rv37471.640.01591
Rv3748hypothetical protein Rv37480.550.00021
Rv3749chypothetical protein Rv3749c0.850.16329
Rv3750chypothetical protein Rv3750c0.910.31656
Rv3751hypothetical protein Rv37511.280.05386
Rv3752chypothetical protein Rv3752c1.440.07038
Rv3753chypothetical protein Rv3753c1.650.10543
Rv3754tyrA1.580.02878
Rv3755chypothetical protein Rv3755c1.120.30586
Rv3756cproZ1.000.49466
Rv3757cproW1.100.13266
Rv3758cproV0.700.02999
Rv3759cproX1.030.39772
Rv3760hypothetical protein Rv37601.920.03904
Rv3761cfadE360.970.25071
Rv3762chypothetical protein Rv3762c0.670.00016
Rv3763lpqH0.990.42272
Rv3764chypothetical protein Rv3764c0.840.19358
Rv3765chypothetical protein Rv3765c1.240.01218
Rv3766hypothetical protein Rv37660.630.00698
Rv3767chypothetical protein Rv3767c1.200.12487
Rv3768hypothetical protein Rv37681.130.13500
Rv3769hypothetical protein Rv37691.260.25175
Rv3770chypothetical protein Rv3770c1.820.10614
Rv3771chypothetical protein Rv3771c1.410.11011
Rv3772hisC21.180.21807
Rv3773chypothetical protein Rv3773c0.690.00037
Rv3774echA210.450.00001
Rv3775lipE1.740.05544
Rv3776hypothetical protein Rv37760.670.00182
Rv3777hypothetical protein Rv37771.070.17981
Rv3778chypothetical protein Rv3778c1.240.12034
Rv3779hypothetical protein Rv37791.890.05477
Rv3780hypothetical protein Rv37801.340.23921
Rv3781hypothetical protein Rv37810.860.29741
Rv3782rfbE1.130.37191
Rv3783hypothetical protein Rv37832.230.09856
Rv3784epiB1.010.48809
Rv3785hypothetical protein Rv37851.780.08031
Rv3786chypothetical protein Rv3786c1.070.38296
Rv3787chypothetical protein Rv3787c3.090.06985
Rv3788hypothetical protein Rv37881.840.09341
Rv3789hypothetical protein Rv37896.830.04745
Rv3790hypothetical protein Rv37900.920.34925
Rv3791hypothetical protein Rv37910.960.45374
Rv3792hypothetical protein Rv37921.020.46276
Rv3793embC0.830.10456
Rv3794embA1.090.34581
Rv3795embB1.080.23968
Rv3796atsH2.150.01247
Rv3797fadE351.380.02072
Rv3798hypothetical protein Rv37981.640.10882
Rv3799caccD40.690.00000
Rv3800cpks131.100.28087
Rv3801cfadD321.230.03936
Rv3802chypothetical protein Rv3802c1.340.09435
Rv3803cfbpC11.500.02447
Rv3804cfbpA0.280.00000
Rv3805chypothetical protein Rv3805c1.120.13868
Rv3806chypothetical protein Rv3806c2.530.08033
Rv3807chypothetical protein Rv3807c1.280.11475
Rv3808chypothetical protein Rv3808c1.170.35706
Rv3809cglf1.200.13034
Rv3810pirG0.890.32587
Rv3811csp2.030.00508
Rv3812PE_PGRS1.190.21438
Rv3813chypothetical protein Rv3813c1.350.08188
Rv3814chypothetical protein Rv3814c0.710.02389
Rv3815chypothetical protein Rv3815c1.230.08452
Rv3816chypothetical protein Rv3816c1.950.07802
Rv3817hypothetical protein Rv38171.540.09835
Rv3818hypothetical protein Rv38180.710.00001
Rv3819hypothetical protein Rv38194.710.03191
Rv3820cpapA23.070.05938
Rv3821hypothetical protein Rv38210.850.00799
Rv3822hypothetical protein Rv38222.470.11809
Rv3823cmmpL80.790.00802
Rv3824cpapA10.960.43143
Rv3825cpks21.740.13457
Rv3826fadD231.140.37656
Rv3827chypothetical protein Rv3827c1.300.06918
Rv3828chypothetical protein Rv3828c0.650.00324
Rv3829chypothetical protein Rv3829c3.490.02860
Rv3830chypothetical protein Rv3830c0.770.04417
Rv3831hypothetical protein Rv38312.930.05827
Rv3832chypothetical protein Rv3832c1.130.34945
Rv3833hypothetical protein Rv38332.460.05261
Rv3834cserS0.810.10145
Rv3835hypothetical protein Rv38351.250.24312
Rv3836hypothetical protein Rv38361.660.13811
Rv3837chypothetical protein Rv3837c1.140.27424
Rv3838cpheA2.240.08402
Rv3839hypothetical protein Rv38392.640.00157
Rv3840hypothetical protein Rv38402.510.05356
Rv3841bfrB0.420.00000
Rv3842cglpQ11.500.02774
Rv3843chypothetical protein Rv3843c0.790.04803
Rv3844hypothetical protein Rv38441.300.06297
Rv3845hypothetical protein Rv38455.030.02550
Rv3846sodA1.630.00106
Rv3847hypothetical protein Rv38471.190.20626
Rv3848hypothetical protein Rv38482.900.00173
Rv3849hypothetical protein Rv38490.580.00000
Rv3850hypothetical protein Rv38501.040.40426
Rv3851hypothetical protein Rv38510.820.00094
Rv3852hns0.870.11172
Rv3853menG0.810.01107
Rv3854chypothetical protein Rv3854c1.930.01334
Rv3855hypothetical protein Rv38551.470.00918
Rv3856chypothetical protein Rv3856c1.280.15202
Rv3857chypothetical protein Rv3857c1.560.03614
Rv3858cgltD1.030.43810
Rv3859cgltB1.090.13995
Rv3860hypothetical protein Rv38600.960.13322
Rv3861hypothetical protein Rv38612.250.03422
Rv3862chypothetical protein Rv3862c5.310.00028
Rv3863hypothetical protein Rv38631.600.01024
Rv3864hypothetical protein Rv38641.130.22402
Rv3865hypothetical protein Rv38651.000.47226
Rv3866hypothetical protein Rv38660.870.00351
Rv3867hypothetical protein Rv38670.970.35508
Rv3868hypothetical protein Rv38681.100.38791
Rv3869hypothetical protein Rv38691.810.04253
Rv3870hypothetical protein Rv38700.950.06023
Rv3871hypothetical protein Rv38711.220.07421
Rv3872PE1.050.24763
Rv3873PPE1.990.00982
Rv3874hypothetical protein Rv38740.400.00002
Rv3875esat60.600.00598
Rv3876hypothetical protein Rv38760.890.29202
Rv3877hypothetical protein Rv38771.720.11673
Rv3878hypothetical protein Rv38781.910.02637
Rv3879chypothetical protein Rv3879c2.210.05255
Rv3880chypothetical protein Rv3880c1.350.22837
Rv3881chypothetical protein Rv3881c0.720.01638
Rv3882chypothetical protein Rv3882c1.070.41036
Rv3883chypothetical protein Rv3883c0.830.24373
Rv3884chypothetical protein Rv3884c0.660.00021
Rv3885chypothetical protein Rv3885c0.850.05166
Rv3886chypothetical protein Rv3886c3.060.04876
Rv3887chypothetical protein Rv3887c1.300.19037
Rv3888chypothetical protein Rv3888c0.580.00001
Rv3889chypothetical protein Rv3889c0.940.11708
Rv3890chypothetical protein Rv3890c1.320.00049
Rv3891chypothetical protein Rv3891c0.850.00616
Rv3892cPPE1.210.20402
Rv3893cPE1.460.01413
Rv3894chypothetical protein Rv3894c5.200.00170
Rv3895chypothetical protein Rv3895c1.460.06505
Rv3896chypothetical protein Rv3896c2.180.00674
Rv3897chypothetical protein Rv3897c1.060.30032
Rv3898chypothetical protein Rv3898c2.180.00067
Rv3899chypothetical protein Rv3899c1.430.07891
Rv3900chypothetical protein Rv3900c2.050.00275
Rv3901chypothetical protein Rv3901c0.810.01548
Rv3902chypothetical protein Rv3902c3.630.22916
Rv3903chypothetical protein Rv3903c0.830.07736
Rv3904chypothetical protein Rv3904c1.230.16778
Rv3905chypothetical protein Rv3905c1.060.33299
Rv3906chypothetical protein Rv3906c0.980.43633
Rv3907cpcnA1.560.02738
Rv3908hypothetical protein Rv39081.270.11454
Rv3909hypothetical protein Rv39091.360.07030
Rv3910hypothetical protein Rv39100.920.18306
Rv3911sigM2.490.03446
Rv3912hypothetical protein Rv39122.680.00309
Rv3913trxB21.720.04598
Rv3914trxC1.800.10738
Rv3915cwlM0.990.47181
Rv3916chypothetical protein Rv3916c0.760.05702
Rv3917cparA1.640.00630
Rv3918cparB3.240.09025
Rv3919cgid1.050.43638
Rv3920chypothetical protein Rv3920c0.650.00038
Rv3921chypothetical protein Rv3921c0.790.14341
Rv3922chypothetical protein Rv3922c0.490.00004
Rv3923crnpA1.010.48559
Rv3924crpmH0.660.00954
|
Each pool was generated by mixing an equal volume of pure culture from each mutant to ensure equal representation. Individual pools were then encapsulated into separate hollow fibers and these were either implanted subcutaneously into mice or incubated in vitro. Hollow fiber contents were recovered on day 1 (input pool) and day 21 (output pool) after infection. PCR amplification of the junction sites between the transposons and the adjacent chromosomal DNA in the pooled genomic DNA from recovered mutants was performed at each time point to determine the presence or absence of each mutant. Of the 100 mutants tested, only that containing a transposon insertion in gene MT2660 (Rv2583c,relMtb) was present in the in vivo input pool, but absent in the in vivo output pool (FIG. 8b). In contrast, this transposon mutant was readily detectable in both input and output pools in vitro (FIG. 8a). Mice were also infected intravenously with pools representing the same 100 transposon mutants. In concordance with findings from the hollow fiber model in vivo, only the relMtb-deficient mutant was found to have significantly decreased survival.
M. tuberculosis deficient in RelMtb, an enzyme responsible for the synthesis and hydrolysis of hyperphosphorylated guanine nucleotides involved in the stringent response, has been shown to be significantly attenuated compared to wild-type in the tissues of mice 38 weeks after aerosol infection. Dahl, J. L., et al. Proc Natl Acad Sci USA 100: 10026-10031 (2003). The growth phenotype of the relMtb transposon insertion mutant (relMtb::Tn), which carries a kanamycin resistance marker, was evaluated in the hollow fiber model in vivo. In order to ensure that each strain was exposed to identical microenvironment conditions, a 1:1 mixture of relMtb::Tn and wild-type strain M. tuberculosis CDC 1551 was encapsulated in hollow fibers and either incubated in liquid broth in vitro or implanted subcutaneously in mice. Twenty-one days later, hollow fibers were recovered and their contents were diluted and plated on antibiotic-free and kanamycin-containing plates to assess CFU counts for each hollow, fiber-encapsulated strain. There was no growth deficit of relMtb::Tn when incubated in vitro, as both hollow fiber-encapsulated wild-type and relMtb::Tn bacilli grew equally after 21 days of incubation (FIG. 8c). However, whereas hollow-fiber encapsulated wild-type bacilli implanted into mice demonstrated reduced growth as compared to those incubated in vitro after 21 days (growth ˜1.5 log for in vivo wild-type vs. ˜3 log10 for in vitro wild-type), hollow fiber-encapsulated relMtb::Tn showed markedly reduced survival as compared to wild-type bacilli implanted in vivo (FIG. 8d). Significantly, the decreased survival of the relMtb-deficient mutant became apparent as early as 21 days after hollow fiber implantation, as compared to several months using the standard murine aerosol infection model. Dahl, J. L., supra. No significant change in survival compared to wild-type in a M. tuberculosis mutant containing a transposon-insertion in an unrelated gene (MT2749, Rv2675) was observed, suggesting that the presence of the transposon insertion alone does not confer a survival disadvantage in the hollow fiber model in vivo.
Gene Expression of Extracellular M. tuberculosis within Granulomas in Mice
The adaptive response of tubercle bacilli in granulomatous lesions in vivo was determined by studying their global gene expression (for complete gene expression profile, see Table 2). Significantly, the exclusion of host cells and containment of bacilli by the hollow fibers renders whole genome microarray analysis feasible. Hollow fibers containing M. tuberculosis were implanted subcutaneously into mice and retrieved 10 days later. Hollow fiber contents were recovered, immediately snap-frozen, and bacilli from 30-40 implanted fibers were pooled to yield sufficient RNA for analysis. Hollow fiber-encapsulated M. tuberculosis gene expression was compared to that of log-phase in vitro-grown M. tuberculosis. Significant differential gene regulation was defined by both a (i) ≧2-fold change in gene expression as compared to control samples and (ii) p-value <0.01.
In vivo hollow fiber-encapsulated M. tuberculosis demonstrates significant induction of ˜260 genes, including several key regulatory genes (see Table 2). Using the strict criteria outlined above, significant upregulation of Rv3133c (dosR) and 20 other members of the recently described dosR regulon (Voskuil, supra), including hspX, Rv2623c, and Rv2626 (Table 3), was observed, suggesting that the adaptive response of hollow fiber-encapsulated M. tuberculosis in vivo is similar to that of bacilli exposed to low concentrations of nitric oxide in vitro.
TABLE 3
|
|
Significantly upregulated dosR regulon genes in the hollow fiber
model in vivo by microarray analysis
P-
Rv No.GeneProtein FunctionFold upregulationvalue
|
80HP2.90.0009
569CHP4.90.007
570nrdZRibonucleotide26.17E−07
reductase cl. II
572cHP2.20.002
1733cCHP30.004
1997ctpFCation transport ATPase13.20.007
2005cCHP-USPA motif2.20.003
2030cCHP7.83.30E−05
2031cacrα-crystallin250.009
2623CHP-USPA motif25.80.0002
2626cCHP14.60.0001
2627cCHP80.008
2628HP8.40.004
2631HP2.10.001
3126cHP2.20.0001
3128cCHP3.20.004
3130cCHP15.40.0003
3131CHP11.40.0008
3132cSensor histidine kinase4.80.001
3133cdosR2-comp. response reg.2.10.007
3134cCHP-USPA motif7.10.007
|
HP = hypothetical protein
|
CHP = conserved hypothetical protein
|
The M. tuberculosis hspX gene encodes α-crystallin, a member of the small heat shock protein family with chaperone activity (Yuan, Y., et al. J Bacteriol 178:4484-4492 (1996)), which is powerfully induced under hypoxic conditions (Sherman, supra), and in lung specimens obtained from patients with active tuberculosis disease. Timm, J., et al. Proc Natl Acad Sci USA 100:14321-14326 (2003). Significantly increased expression of hspX, Rv2623c, and Rv2626 has been shown by real time RT-PCR after the onset of Th1-mediated immunity in the mouse model of tuberculosis (Shi, supra) and in various models of latency in vitro. Sherman, supra, Betts, supra, Voskuil, supra, Shi, supra. In addition, upregulation of 12 additional genes of the dosR regulon which fulfilled one, but not both criteria listed above was also observed (see Table 2). Hollow fiber-encapsulated M. tuberculosis in vivo also demonstrated significant upregulation of sigB, sigC, and sigH, which belong to a family of alternative RNA polymerase sigma factors shown to coordinate gene regulation in response to environmental conditions in M. tuberculosis and other bacterial species. Haldenwang, W. G. Tuber Lung Dis 78:175-183 (1995), Gomez, et al., Tuber Lung Dis, 78:175-183 (1997). Significant upregulation of dnaE2, which encodes a DNA polymerase in M. tuberculosis and in other organisms, and which has been shown to be upregulated by several DNA damaging agents and during infection of mice, was detected, contributing to in vivo survival and the emergence of drug resistance. Boshoff, H. I., et al. Cell 113:183-193 (2003). In addition, hollow fiber-encapsulated M. tuberculosis in mice demonstrated significant induction of many other genes recently found to be upregulated in the multibacillary model of murine tuberculosis (Talaat, supra), including RV0967, Rv0970, Rv0978c (PE-PGRS 17), Rv0980c (PE-PGRS 18), Rv982 (mprB), and Rv0988.
Consistent with evidence that M. tuberculosis might switch to C2 carbon sources such as fatty acids during in vivo persistence (McKinney, J. D., et al. Nature 406:735-738 (2000)), significant upregulation of several genes encoding enzymes involved in lipid degradation, including fadD10 (Rv0099), fadD19 (Rv3515c), fadD34 (Rv0035), fadE1 (Rv013 1 c), fadE10 (Rv0873), and fadE24 (Rv3139) was also detected. In addition, significantly increased expression of pckA (Rv0211) was observed, suggesting limited availability of glucose within artificial granulomas in mice. De novo synthesis of glucose-6-phosphate in mycobacteria is achieved through the action of phosphoenolpyruvate carboxykinase (PckA), which converts oxaloacetate to phosphoenolpyruvate, thus diverting carbon derived from β-oxidation of fatty acids into gluconeogenesis. Tuckman, D., et al. J Bacteriol 179:2724-2730 (1997). Increased gene expression of pckA has been reported in the mouse model of tuberculosis (Timm, supra), and disruption of pckA in M. bovis produced attenuated strains in animal models of infection. Liu, K., et al. Microbiology 149:1829-1835 (2003), Collins, D. M., et al. Microbiology 148:3019-3027 (2002). In addition, a 1.5-fold induction of Rv0467 (aceA) (p=0.004) was found, which encodes isocitrate lyase, the first enzyme of the glyoxylate cycle, which is strongly induced in mouse lungs (Timm, supra) and is required for long-term persistence of M. tuberculosis in mice. McKinney, supra.
Consistent with the hypothesis that hollow fiber-encapsulated bacilli in vivo display a reduced replication rate, significantly decreased expression of certain genes encoding ribosomal RNA binding proteins, including Rv0701 (rplC), Rv0703 (rplW), Rv0704 (rplB), Rv0706 (rplV), Rv0708 (rplP), Rv0710 (rplQ), and Rv0716 (rplE) was observed. In agreement with the luciferase assay data suggesting that these bacilli have decreased intracellular energy stores (FIG. 4b), significant downregulation of several genes encoding components of ATP synthase, including Rv1304 (atpB), Rv1306 (atpF), Rv1308 (atpA), Rv1310 (atpD), and Rv1311 (atpC) was found. A complete list of significantly downregulated genes in hollow fiber-encapsulated M. tuberculosis implanted into mice can be found in Table 2. Differential regulation of a subset of 13 genes identified by microarray analysis was confirmed using quantitative real-time RT-PCR (Table 4).
TABLE 4
|
|
Confirmation of differential regulation of a subset of genes by
quantitative real-time RT-PCR
Rv No.GeneProtein FunctionFold induction
|
1997HP32
2031cacrα-crystallin294.1
2623CHP-USPA motif512
2626cCHP68.6
2710sigBRNA polymerase sigma42.2
factor
2744c35 kd_Ag4.3
3130cCHP168.9
3131CHP274.3
3133cdosR2-comp. response regulator78.8
3223csigHRNA polymerase sigma17.1
factor
3370cdnaE2DNA polymerase III9.8
3477PE−4.3
3648ccspAprob. cold shock protein−7
|
HP = hypothetical protein
|
CHP = conserved hypothetical protein
|
Discussion
The hollow fiber encapsulation/implantation technique provides a means to establish a paucibacillary infection with M. tuberculosis in which the bacilli are readily recoverable from infected animals for further analysis. In this model of infection, the tubercle bacilli rapidly enter an altered physiologic state characterized by stationary-state CFU counts and decreased metabolic activity. These bacilli are more susceptible to the antituberculous drug rifampin than they are to isoniazid, consistent with the antibiotic susceptibility profile of persistent bacilli in animal chemotherapy models (Lecoeur, supra, Dickinson, supra) and in human latent tuberculosis infection. Am Rev Respir Dis 145:36-41 (1992), Jasmer, supra, Fox, supra. In addition, expression of relMtb, a gene which is essential for long-term persistence in the mouse model of chronic tuberculosis (Dahl, supra), is also necessary for short-term bacillary persistence in the hollow fiber granuloma model in vivo, suggesting a common adaptive strategy of M. tuberculosis in these two models of infection. These results demonstrate induction of dosR (Rv3133c) and 20 other members of the dosR regulon believed to mediate the transition into dormancy, and that relMtb is required for M. tuberculosis survival during extracellular persistence within host granulomas. Interestingly, the dormancy phenotype of extracellular M. tuberculosis within host granulomas appears to be immune-mediated and interferon-gamma-dependent.
In this example, the whole-genome transcriptional profile of extracellular M. tuberculosis within granulomas in mice is presented. In vivo hollow fiber-encapsulated M. tuberculosis demonstrates significant induction of several key regulatory genes, including Rv3133c (dosR), as well as 20 other genes of the recently described dosR regulon (Voskuil, supra), the sigma factor genes sigB, sigC, and sigH, the DNA polymerase-encoding dnaE2, and many other genes which were found to be significantly upregulated in the mouse model of pulmonary tuberculosis. Talaat, supra. Increased expression of genes involved in lipid metabolism suggests depletion of glucose and the importance of fatty acids as a primary carbon source for extracellular M. tuberculosis in artificial granulomas in mice, which corroborates previous data in the mouse model of chronic tuberculosis. McKinney, supra. Consistent with the hypothesis that these organisms demonstrate an altered physiologic state characterized by reduced replication and metabolism, significant downregulation of genes encoding key ribosomal RNA binding proteins, and ATP synthase, respectively, was detected. A subset of 13 genes identified by microarray analysis was confirmed to be significantly differentially regulated by quantitative real-time RT-PCR. The common transcriptional profile observed lends further support to the hypothesis that the altered physiologic state of hollow fiber-encapsulated bacilli in vivo is similar to that of persistent bacilli in other models of latency.
It is important to note that although the artificial subcutaneous granulomas surrounding hollow fibers containing M. tuberculosis resemble those formed in mouse lungs after aerosol infection with the same pathogen, granulomas in mice differ significantly from those in humans. In human infection, the granuloma is composed of a central core of macrophages, including multinucleated giant cells, surrounded by macrophages and lymphocytes, including CD4 and CD8 T cells, and B cells. Randhawa, P. S. Pathology 22:153-155 (1990). Although individual components of mouse granulomas are similar to those in humans, the architecture of the mouse granuloma is better characterized as a loose collection of activated and epithelioid macrophages and lymphocytic clusters. Flynn, J. L., and J. Chan. Animal models of tuberculosis. In Tuberculosis. G. S M, editor. Lippicott Williams & Wilkins, Philadelphia. 237-250 (2004). Unlike in human granulomas, multinucleated giant cells are absent in mouse granulomas, and necrosis and caseation are rarely observed. Id. Despite structural differences between granulomas in mice and humans, however, their function is likely to be similar, with respect to containment of infection and creation of a localized environment for the immune response to kill organisms.
Animal models of tuberculosis have shown that M. tuberculosis is an intracellular pathogen, residing within host macrophages. Flynn, supra. However, the precise location of persistent bacilli during human latent tuberculosis infection remains elusive. Specifically, autopsy data of persons who died of non-tuberculosis-related causes have demonstrated that tubercle bacilli may be found outside lung granulomas, in normal-appearing lung tissue (Opie E L, A. J. Arch Pathol Lab Med 4:1-21 (1927)), and in non-macrophage cell types, including alveolar epithelial cells. Hemandez-Pando, R., et al. Lancet 356:2133-2138 (2000). In addition, bacilli residing extracellularly in the caseous material of lung granulomas and cavities may represent an important reservoir of persistent organisms during human latent tuberculosis infection. Grosset, supra. Mouse-implanted hollow fibers containing M. tuberculosis induce splenomegaly and the accumulation of host inflammatory cells, including macrophages and lymphocytes, leading to the formation of artificial granulomas around the fibers. The precise microenvironment conditions prevailing within subcutaneously-implanted hollow fibers are unclear, but may include hypoxia, nutrient starvation, and soluble immunologic factors (e.g., diffusible nitric oxide) secreted by surrounding host immune cells. Diminished ability to contain the growth of hollow fiber-encapsulated M. tuberculosis by IFNγ-deficient mice, as well as significant induction of the dosR regulon by these bacilli suggests that diffusible nitric oxide and/or hypoxia may contribute to the microenvironment experienced by infra-fiber bacilli in vivo. The basis for mouse spleen enlargement and recruitment of host immune cells to the tissues surrounding the hollow fibers is unknown, but may involve diffusion of M. tuberculosis-secreted soluble factors across the hollow fiber membranes.
As used herein, the terms “about” and “approximately” when referring to a numerical value shall have their plain and ordinary meanings to one of ordinary skill in the art. The amount of broadening from the strict numerical boundary depends upon the criticality of the particular element at issue. Thus, as a general matter, “about” or “approximately” broaden the numerical value, yet cannot be given a precise limit. For example, in some cases, “about” or “approximately” may mean ±5%, or ±10%, or ±20%, or ±30%, or ±100% depending on the relevant technology and the effects the variances will elicit.
A number of embodiments of the invention have been described. Nevertheless, it will be understood that various modifications may be made without departing from the spirit and scope of the invention. Accordingly, other embodiments are within the scope of the following claims, and as various changes can be made to the above compositions, formulations, combinations, methods, and processes without departing from the scope of the invention. It is intended that all matter contained in the above description be interpreted as illustrative and not in a limiting sense.
All publications cited herein are hereby incorporated by reference to the same extent as if each publication were individually and specifically indicated to be incorporated by reference and were set forth in its entirety herein.