Patent Application
20250179023
The present invention belongs to the field of pharmaceutical synthesis, and particularly relates to a holosymmetric biphenyl derivative and a preparation method therefor and use thereof.
The immune system plays a very important role in controlling and eliminating diseases, such as cancer. However, tumor cells are often able to develop a strategy for escaping or suppressing the monitoring of the immune system to promote their malignant growth. One very important mechanism is to change the expression of co-stimulatory and co-inhibitory immune checkpoint molecules on immunocytes. Blocking the signal pathway of immune checkpoint molecules, such as PD1, has been proved to be an extremely promising and effective therapy.
Programmed cell death protein 1 (PD-1), also known as CD279, is a receptor expressed on the surfaces of activated T cells, natural killer T cells, B cells and macrophages. Its structure contains an extracellular domain similar to an immunoglobulin variable region, a transmembrane domain and an intracellular domain, where the intracellular domain contains two phosphorylation sites located in an immunoreceptor tyrosine kinase-based inhibitory domain and an immunoreceptor tyrosine kinase-based transduction domain, suggesting that PD1 can down-regulate T cell receptor-mediated signal pathways.
PD1 has two ligands: PD-L1 and PDL2, and they are different in their expression profiles. The expression of PDL1 will be up-regulated in macrophages and dendritic cells after treatment with lipopolysaccharide (LPS) and granulocyte-macrophage colony-stimulating factor (GM-CSF), and will also be up-regulated in T cells and B cells after stimulation of T cell receptor and B cell receptor signal pathways. PDL1 is also highly expressed in almost all tumor cells, and the expression will be up-regulated after stimulation of interferon (IFN) gamma. As a matter of fact, the expression of PDL1 in a variety of tumors is considered to have prognostic relevance; but, the expression of PD-L2 is relatively concentrated, mainly on dendritic cells.
When T cells expressing PD-1 come into contact with cells expressing the ligands of PD-1, those antigen-stimulated functional activities, such as cell proliferation, cytokine release and cell lysis activity, are all inhibited. Therefore, the interaction between PD1 and ligands thereof can function as an intrinsic negative feedback regulation mechanism to prevent T cell hyperactivation during infection, immune tolerance or tumorigenesis, thus reducing the occurrence of autoimmune diseases and promoting self tolerance. Long-term antigenic stimulation, e.g., in tumor or long-term infection, will cause T cells to express high level of PD-1, gradually lose activities in response to these long-term antigens, and eventually become nonfunctional, namely, the so-called T cell exhaustion. B cells also have the inhibitory effect caused by PD1 and ligands thereof and corresponding functional exhaustion.
Some evidence from preclinical animal studies has indicated that PD-1 and ligands thereof can down-regulate the immunoreaction. PD-1-deficient mice will develop lupus erythematosus-like acute proliferative glomerulonephritis and dilated cardiomyopathy. Utilizing the antibody of PDL1 to block PD-1/PDL1 interaction has been proved to be able to restore and enhance T cell activation in many systems. The monoclonal antibody of PDL1 can also benefit patients with advanced cancers. Some preclinical animal tumor models also show that blocking the signal pathway of PD-1/PD-L1 with a monoclonal antibody can enhance immunoreaction and lead to immunoreactions to a series of histologically different tumors. With the long-term infection LCMV model, the PD-1/PD-L1 interaction has been found to be able to inhibit the activation and proliferation of virus-specific CD8 T cells and the acquisition of effector cell functions. Besides being capable of enhancing the immunoreaction to long-term antigens, blocking the pathway of PD-1/PDL1 has also been found to be able to enhance response to vaccines, including response to a therapeutic vaccine in long-term infection.
To sum up, if, besides the existing monoclonal antibodies, a compound for blocking the PD-1/PD-L1 interaction can be developed, it can serve as an effective therapeutic means for blocking the PD-1/PDL1-mediated inhibitory signal pathway to enhance or restore the function of T cells. Therefore, the compound specifically blocking the PD-1/PD-L1 interaction will achieve a good therapeutic effect in immunotherapies for a variety of cancers and other immunity-related diseases.
The object of the present invention is to provide an overall holosymmetric biphenyl derivative, and a preparation method therefor and use thereof, so that a new generation of PD-1/PD-L1 inhibitors are expected to be developed.
A first aspect of the present invention provides a compound of formula (I), a stereoisomer or pharmaceutically acceptable salt thereof:
As a preferred embodiment, in the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof, both R1 are identical or different and are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, C1-4 alkyl, C1-4 haloalkyl, C1-4 deuterioalkyl, cyclopropyl-substituted C1-4 alkyl and C3-6 cycloalkyl.
As a further preferred embodiment, in the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof, both R1 are identical or different and are each independently selected from the group consisting of hydrogen, deuterium, fluoro, chloro, cyano, methyl, ethyl, isopropyl, trifluoromethyl, trideuteriomethyl, cyclopropylmethyl and cyclopropyl.
As a preferred embodiment, in the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof, both R2 are identical or different and are each independently selected from the group consisting of hydrogen, deuterium, C1-4 alkyl, C1-4 haloalkyl, C1-4 deuterioalkyl, cyclopropyl-substituted C1-4 alkyl, C3-6 cycloalkyl and hydroxy.
As a further preferred embodiment, in the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof, both R2 are identical or different and are each independently selected from the group consisting of hydrogen, deuterium, methyl, ethyl, isopropyl, trifluoromethyl, trideuteriomethyl, cyclopropylmethyl, cyclopropyl and hydroxy.
As a preferred embodiment, in the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof, both R3 are identical or different and are each independently selected from the group consisting of hydrogen, deuterium, C1-4 alkyl, C3-6 cycloalkyl and 3-6 membered heterocyclyl, the C1-4 alkyl, C3-6 cycloalkyl and 3-6 membered heterocyclyl are further optionally substituted by one or more substituents selected from the group consisting of deuterium, halogen, cyano, hydroxy, carboxyl, C1-4 alkyl, C1-4 haloalkyl, C1-4 deuterioalkyl, C3-6 cycloalkyl, 3-6 membered heterocyclyl, C3-6 cycloalkoxy and C1-4 alkoxy.
As a further preferred embodiment, in the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof, both R3 are identical or different and are each independently selected from the group consisting of hydrogen, deuterium, methyl, ethyl, isopropyl, cyclopropyl, cyclobutyl, cyclopentyl, oxacyclobutyl and azacyclobutyl, the methyl, ethyl, isopropyl, cyclopropyl, cyclobutyl, oxacyclobutyl and azacyclobutyl are further optionally substituted by one or more substituents selected from the group consisting of deuterium, halogen, cyano, hydroxy, carboxyl, methyl, ethyl, isopropyl, trifluoromethyl, trideuteriomethyl, cyclopropyl, cyclobutyl, oxacyclobutyl, azacyclobutyl, cyclopropyloxy, cyclobutyloxy, methoxy, ethoxy and isopropoxy.
As a more further preferred embodiment, in the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof, both R3 are identical or different and are each independently selected from the group consisting of hydrogen, deuterium, methyl, ethyl, isopropyl, trifluoromethyl, trideuteriomethyl, cyclopropylmethyl, cyclopropyl, cyclobutyl, oxacyclobutyl and azacyclobutyl.
As a more further preferred embodiment, in the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof, both R1 are identical and are selected from the group consisting of hydrogen, deuterium, fluoro, chloro, cyano, methyl, ethyl, isopropyl, trifluoromethyl, trideuteriomethyl, cyclopropylmethyl and cyclopropyl;
As the most preferred embodiment, the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof include, but are not limited to, the following compounds:
A second aspect of the present invention provides a pharmaceutical composition comprising the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof described above and a pharmaceutically acceptable carrier.
A third aspect of the present invention provides use of the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof in the preparation of a medicament for treating a PD-1/PD-L1 signal pathway-mediated disease.
As a preferred embodiment, the PD-1/PD-L1 signal pathway-mediated disease is cancer or tumor, immune-related disease and disorder, communicable disease, infectious disease or metabolic disease.
As a further preferred embodiment, the cancer or tumor is lymphoma (including but not limited to lymphocytic lymphoma, primary central nervous system lymphoma, T cell lymphoma, diffuse large B cell lymphoma, follicle center lymphoma, Hodgkin lymphoma, non-Hodgkin lymphoma or primary mediastinal large B cell lymphoma), sarcoma (including but not limited to Kaposi's sarcoma, fibrosarcoma, liposarcoma, chondrosarcoma, osteosarcoma, leiomyosarcoma, rhabdomyosarcoma, soft tissue sarcoma, angiosarcoma or lymphangiosarcoma), melanoma, glioblastoma, synovioma, meningioma, biliary tract tumor, thymic tumor, neuroma, seminoma, nephroblastoma, pleomorphic adenoma, hepatocellular papilloma, renal tubule adenoma, cystadenoma, papilloma, adenoma, leiomyoma, rhabdomyoma, hemangioma, lymphangioma, osteoma, chondroma, lipoma, fibroma, central nervous system tumor, rhachiophyma, brain stem glioma, pituitary adenoma, multiple myeloma, ovarian tumor, myelodysplastic syndrome or mesothelioma, prostate cancer, recurrent prostate cancer or prostate cancer having developed resistance to existing medicaments, thyroid cancer, parathyroid cancer, anal cancer, testicular cancer, urethral carcinoma, penile cancer, bladder cancer, ureteral cancer, uterine cancer, ovarian cancer, fallopian tube cancer, endometrial cancer, cervical cancer, vaginal cancer, vulvar cancer, adrenal cancer, Merkel cell carcinoma, embryonal carcinoma, chronic or acute leukemia (including but not limited to acute myeloid leukemia, chronic myeloid leukemia, acute lymphoblastic leukemia, chronic granulocytic leukemia and chronic lymphoblastic leukemia), bronchial carcinoma, esophageal cancer, nasopharyngeal carcinoma, hepatocellular carcinoma, renal cell carcinoma, small cell lung cancer, basal cell carcinoma, lung cancer, breast cancer, adenocarcinoma, papillary carcinoma, cystadenocarcinoma, squamous non-small cell lung cancer, non-squamous non-small cell lung cancer, rectal cancer, colon cancer, colorectal cancer, gastric cancer, pancreatic cancer, head and neck squamous cell carcinoma, head and neck cancer, gastrointestinal cancer, bone cancer, skin cancer, small intestine cancer, endocrine cancer, renal pelvic carcinoma, epidermoid carcinoma, abdominal wall carcinoma, renal cell carcinoma, transitional cell carcinoma, choriocarcinoma or metastatic tumor;
In a fourth aspect, the present invention provides the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof for use as a medicament for treating PD-1/PD-L1 signal pathway-mediated cancer or tumor, immune-related disease and disorder, communicable disease, infectious disease or metabolic disease.
The present invention also relates to a method for treating PD-1/PD-L1 signal pathway-mediated cancer or tumor, immune-related disease and disorder, communicable disease, infectious disease or metabolic disease, which comprises administering to a patient in need thereof a clinically effective amount of the compound of formula (I), the stereoisomer or pharmaceutically acceptable salt thereof.
After extensive and intensive research, the inventors of the present invention, for the first time, developed a biphenyl series of compounds having the structure of general formula (I). The series of compounds of the present invention have a strong inhibitory effect on the PD-1/PD-L1 interaction, and they can be widely used in the preparation of a drug for preventing and/or treating PD-1/PD-L1 signal pathway-mediated cancer or tumor, immune-related disease and disorder, communicable disease, infectious disease or metabolic disease and are expected to be developed into a new generation of PD-1/PD-L1 inhibitors. The present invention is achieved on this basis.
Detailed description: unless otherwise stated or specified, the following terms used in the specification and claims have the following meanings.
“Alkyl” refers to linear or branched saturated aliphatic alkyl groups, preferably linear alkyl or branched alkyl containing 1 to 10 or 1 to 4 carbon atoms, including but not limited to methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl, n-pentyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, 2-methylbutyl, 3-methylbutyl, n-hexyl, 1-ethyl-2-methylpropyl, 1,1,2-trimethylpropyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1,3-dimethylbutyl, 2-ethylbutyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 2,3-dimethylbutyl, n-heptyl, 2-methylhexyl, 3-methylhexyl, 4-methylhexyl, 5-methylhexyl, 2,3-dimethylpentyl, 2,4-dimethylpentyl, 2,2-dimethylpentyl, 3,3-dimethylpentyl, 2-ethylpentyl, 3-ethylpentyl, n-octyl, 2,3-dimethylhexyl, 2,4-dimethylhexyl, 2,5-dimethylhexyl, 2,2-dimethylhexyl, 3,3-dimethylhexyl, 4,4-dimethylhexyl, 2-ethylhexyl, 3-ethylhexyl, 4-ethylhexyl, 2-methyl-2-ethylpentyl, 2-methyl-3-ethylpentyl or various branched isomers thereof, and the like. “C1-10 alkyl” refers to linear alkyl and branched alkyl containing 1 to 10 carbon atoms, and “C1-4 alkyl” refers to linear alkyl and branched alkyl containing 1 to 4 carbon atoms.
Alkyl may be optionally substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Cycloalkyl” or “carbocycle” refers to a monocyclic or polycyclic hydrocarbon substituent that is saturated or partially unsaturated. The partially unsaturated cyclic hydrocarbon means that the cyclic hydrocarbon may contain one or more (preferably 1, 2 or 3) double bonds, but none of the rings has a fully conjugated π-electron system; cycloalkyl is classified into monocyclic cycloalkyl and polycyclic cycloalkyl and is preferably cycloalkyl containing 3 to 10 or 3 to 6 carbon atoms. For example, “C3-10 cycloalkyl” refers to cycloalkyl containing 3 to 10 carbon atoms, and “C3-6 cycloalkyl” refers to cycloalkyl containing 3 to 6 carbon atoms, wherein
“Fused cycloalkyl” refers to an all-carbon polycyclic group in which each ring shares a pair of adjacent carbon atoms with the other rings in the system, wherein one or more of the rings may contain one or more (preferably, 1, 2 or 3) double bonds, but none of them has a fully conjugated π-electron system. According to the number of formed rings, the fused cycloalkyl may be bicyclic, tricyclic, tetracyclic or polycyclic, including but not limited to:
“Bridged cycloalkyl” refers to an all-carbon polycyclic group in which any two rings share two carbon atoms that are not directly connected to each other, wherein these rings may contain one or more (preferably, 1, 2 or 3) double bonds, but none of them has a fully conjugated π-electron system. According to the number of formed rings, the bridged cycloalkyl may be bicyclic, tricyclic, tetracyclic or polycyclic, including but not limited to:
The cycloalkyl ring can be fused to an aryl, heteroaryl or heterocycloalkyl ring, wherein the ring attached to the parent structure is cycloalkyl, which includes, but is not limited to, indanyl, tetrahydronaphthyl, benzocycloheptyl and the like.
“Cycloalkyl” or “carbocycle” may be optionally substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Heterocyclyl” or “heterocycle” refers to a monocyclic or polycyclic hydrocarbon substituent that is saturated or partially unsaturated. The partially unsaturated cyclic hydrocarbon means that the cyclic hydrocarbon may contain one or more (preferably 1, 2 or 3) double bonds, but none of the rings has a fully conjugated π-electron system. One or more (preferably 1, 2, 3 or 4) ring atoms in the heterocyclyl are selected from heteroatoms N, O, N.O and S(O)r (where r is an integer of 0, 1 or 2), excluding ring moiety of —O—O—, —O—S— or —S—S—, and the remaining ring atoms are carbon atoms. Preferred is heterocyclyl comprising 3 to 10 or 3 to 6 ring atoms, e.g. “3-6 membered heterocyclyl” refers to heterocyclyl comprising 3 to 6 ring atoms, and “3-10 membered heterocyclyl” refers to heterocyclyl comprising 3 to 10 ring atoms.
Monocyclic heterocyclyl includes, but is not limited to, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, homopiperazinyl, oxetanyl, tetrahydrofuranyl and the like.
Polycyclic heterocyclyl includes spiroheterocyclyl, fused heterocyclyl and bridged heterocyclyl. “Spiroheterocyclyl” refers to a polycyclic heterocyclyl group in which an atom (called a spiro-atom) is shared among monocyclic rings, wherein one or more (preferably 1, 2, 3 or 4) ring atoms are heteroatoms selected from N, O, N.O and S(O)r (where r is an integer of 0, 1 or 2), and the remaining ring atoms are carbon atoms. These rings may contain one or more (preferably, 1, 2 or 3) double bonds, but none of them has a fully conjugated π-electron system. According to the number of spiro-atoms shared among the rings, the spiroheterocyclyl may be monospiroheterocyclyl, bispiroheterocyclyl or polyspiroheterocyclyl. Spiroheterocyclyl includes, but is not limited to:
“Fused heterocyclyl” refers to a polycyclic heterocyclyl group in which each ring shares a pair of adjacent atoms with the other rings in the system, wherein one or more (preferably, 1, 2, 3 or 4) of the rings may contain one or more (preferably, 1, 2 or 3) double bonds, but none of them has a fully conjugated π-electron system, wherein one or more (preferably, 1, 2, 3 or 4) ring atoms are heteroatoms selected from N, O, N.O and S(O)r (where r is an integer of 0, 1 or 2), and the remaining ring atoms are carbon atoms. According to the number of formed rings, the fused heterocyclyl may be bicyclic, tricyclic, tetracyclic or polycyclic, including, but not limited to:
“Bridged heterocyclyl” refers to a polycyclic heterocyclyl group in which any two rings share two carbon atoms that are not directly connected to each other, wherein these rings may contain one or more (preferably, 1, 2 or 3) double bonds, but none of them has a fully conjugated π-electron system, wherein one or more (preferably, 1, 2, 3 or 4) ring atoms are heteroatoms selected from N, O, N.O and S(O)r (where r is an integer of 0, 1 or 2), and the remaining ring atoms are carbon atoms. According to the number of formed rings, the bridged heterocyclyl may be bicyclic, tricyclic, tetracyclic or polycyclic, including but not limited to:
The heterocyclyl ring may be fused to an aryl, heteroaryl or cycloalkyl ring, wherein the ring attached to the parent structure is heterocyclyl, including but not limited to:
“Heterocyclyl” or “heterocycle” may be optionally substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Aryl” or “aromatic ring” refers to an all-carbon monocyclic or fused-polycyclic (i.e., rings that share a pair of adjacent carbon atoms) group and a polycyclic (i.e., rings with adjacent pairs of carbon atoms) group having a conjugated π-electron system, preferably all-carbon aryl containing 6 to 10 carbon atoms; for example, “C6-10 aryl” refers to all-carbon aryl containing 6 to 10 carbon atoms, including but not limited to, phenyl and naphthyl. The aryl ring can be fused to a heteroaryl, heterocyclyl or cycloalkyl ring, wherein the ring attached to the parent structure is the aryl ring, including but not limited to:
“Aryl” or “aromatic ring” may be substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Heteroaryl” or “heteroaromatic ring” refers to a heteroaromatic system containing one or more (preferably 1, 2, 3 or 4) heteroatoms including N, O, N.O and S(O)r (where r is an integer of 0, 1 or 2), and is preferably a heteroaromatic system containing 5 to 10 or 5 to 8 ring atoms. For example, “5-8 membered heteroaryl” refers to a heteroaromatic system containing 5 to 8 ring atoms, and “5-10 membered heteroaryl” refers to a heteroaromatic system containing 5 to 10 ring atoms. The heteroaryl includes, but is not limited to, furyl, thiophenyl, pyridyl, pyrrolyl, N-alkylpyrrolyl, pyrimidinyl, pyrazinyl, imidazolyl, tetrazolyl and the like. The heteroaryl ring can be fused to an aryl, heterocyclyl or cycloalkyl ring, wherein the ring attached to the parent structure is the heteroaryl ring, including but not limited to:
“Heteroaryl” or “heteroaryl ring” may be optionally substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Alkenyl” refers to alkyl defined as above consisting of at least two carbon atoms and at least one carbon-carbon double bond, and is preferably linear or branched alkenyl containing 2 to 10 carbon atoms. For example, “C2-10 alkenyl” refers to linear or branched alkenyl containing 2 to 10 carbon atoms. Alkenyl includes, but is not limited to, vinyl, 1-propenyl, 2-propenyl, 1-, 2- or 3-butenyl, and the like.
“Alkenyl” may be substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Alkynyl” refers to alkyl defined as above consisting of at least two carbon atoms and at least one carbon-carbon triple bond, and is preferably linear or branched alkynyl containing 2 to 10 carbon atoms. For example, “C2-10 alkynyl” refers to linear or branched alkynyl containing 2 to 10 carbon atoms. Alkynyl includes, but is not limited to, ethynyl, 1-propynyl, 2-propynyl, 1-, 2- or 3-butynyl, and the like.
“Alkynyl” may be substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Alkoxy” refers to —O-(alkyl), wherein the alkyl is defined as above. For example, “C1-4 alkoxy” refers to alkoxy containing 1 to 4 carbons atoms. Alkoxy includes, but is not limited to, methoxy, ethoxy, propoxy, butoxy, and the like.
“Alkoxy” may be optionally substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Cycloalkoxy” or “cycloalkyloxy” refers to —O-cycloalkyl, wherein the cycloalkyl is defined as above. For example, “C3-6 cycloalkoxy” refers to cycloalkyloxy containing 3 to 6 carbon atoms. Cycloalkoxy or cycloalkyloxy includes, but is not limited to, cyclopropyloxy, cyclobutyloxy, cyclopentyloxy, cyclohexyloxy, etc.
“Cycloalkoxy” or “cycloalkyloxy” may be optionally substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“Heterocycloxy” or “heterocyclyloxy” refers to-O-heterocyclyl, wherein the heterocyclyl is defined as above, and heterocyoxy or heterocyclyloxy includes, but is not limited to, azacyclobutyloxy, oxacyclobutyloxy, azacyclopentyloxy, nitrogen, oxacyclohexyloxy and the like.
“Heterocycloxy” or “heterocyclyloxy” may be optionally substituted or unsubstituted, and when it is substituted, the substituents are preferably one or more (preferably 1, 2, 3 or 4) groups independently selected from the group consisting of deuterium, halogen, cyano, nitro, azido, C1-10 alkyl, C1-10 haloalkyl, C1-10 deuterioalkyl, C2-10 alkenyl, C2-10 alkynyl, C3-10 cycloalkyl, 3-10 membered heterocyclyl, C6-10 aryl, 5-10 membered heteroaryl, ═O, ═S and —SF5.
“C1-4 haloalkyl” refers to an alkyl group having 1 to 4 carbon atoms in which hydrogen on the alkyl is optionally substituted with a fluorine, chlorine, bromine or iodine atom, and includes, but is not limited to, difluoromethyl, dichloromethyl, dibromomethyl, trifluoromethyl, trichloromethyl, tribromomethyl and the like.
“C1-4 deuterioalkyl” refers to an alkyl group having 1 to 4 carbon atoms in which hydrogen on the alkyl is optionally substituted with a deuterium atom, and includes, but is not limited to, monodeuteriomethyl, dideuteriomethyl, trideuteriomethyl and the like.
“Halogen” refers to fluoro, chloro, bromo or iodo, “NaOH” refers to sodium hydroxide, “BH3.Me2S” refers to borane dimethylsulfide, “MeOH” refers to methanol, “NaBH3CN” refers to sodium cyanoborohydride, “HOAc” refers to acetic acid, “DMF” refers to N,N-dimethylformamide, and “THF” refers to tetrahydrofuran.
The term “optional” or “optionally” means that the event or circumstance subsequently described may, but not necessarily, occur, and that the description includes instances where the event or circumstance occurs or does not occur, that is, instances where substitution occurs or does not occur. For example, “heterocyclyl group optionally substituted with alkyl” means that alkyl may be, but not necessarily, present, and that the description includes instances where the heterocyclyl group is or is not substituted with alkyl.
The term “substituted” means that one or more “hydrogen atoms” in the group are each independently substituted by a corresponding number of substituents. It goes without saying that a substituent is only in its possible chemical position and consistent with chemical valence bond theory, and those skilled in the art will be able to determine (by studies or theories) possible or impossible substitution without undue efforts. For example, it may be unstable when amino or hydroxy having free hydrogen is bound to a carbon atom having an unsaturated bond (such as olefin).
“Stereoisomers” refer to isomers produced by different spatial arrangements of atoms in molecules, and can be classified into cis-trans isomers and enantiomers, and also into enantiomers and diastereomers. Stereoisomers resulting from rotation of single bonds are referred to as conformational stereo-isomers and sometimes also as rotamers. Stereoisomers resulting from bond lengths, bond angles, intramolecular double bonds, rings and the like are referred to as configuration stereo-isomers, and the configuration stereo-isomers are classified into two categories. Among them, isomers resulting from the fact that a double bond or a single bond of a ring-forming carbon atom cannot rotate freely are referred to as geometric isomers and also as cis-trans isomers, and the isomers are classified into Z and E configurations. For example, cis-2-butene and trans-2-butene are a pair of geometric isomers, and stereoisomers having different optical rotation properties due to the absence of anti-axisymmetry in the molecule are referred to as optical isomers, and are classified into R and S configurations. In the present invention, the term “stereoisomer” is understood to include one or more of the above enantiomers, configuration stereo-isomers and conformational stereo-isomers, unless otherwise specified.
“Pharmaceutically acceptable salt” used herein refers to pharmaceutically acceptable acid addition salts, including inorganic and organic acid salts, which may be prepared by methods known in the art.
“Pharmaceutical composition” refers to a mixture containing one or more of the compounds described herein or a physiologically/pharmaceutically acceptable salt or pro-drug thereof, and other chemical components, for example, physiologically/pharmaceutically acceptable carriers and excipients. The purpose of the pharmaceutical composition is to promote the administration to an organism, which facilitates the absorption of the active ingredient, thereby exerting biological activities.
The present invention is further explained in detail below with reference to examples, which are not intended to limit the present invention, and the present invention is not merely limited to the contents of the examples.
The compound structure of the present invention is determined by nuclear magnetic resonance (NMR) and/or liquid chromatography-mass spectrometry (LC-MS). The NMR chemical shift (δ) is given in parts per million (ppm). The NMR determination is conducted by using Bruker AVANCE-400/500 nuclear magnetic resonance apparatus, with hexadeuterodimethyl sulfoxide (DMSO-d6), tetradeuteromethanol (CD3OD), and deuterated chloroform (CDCl3) as determination solvents, and tetramethylsilane (TMS) as internal standard.
The LC-MS determination is conducted by using an Agilent 6120 mass spectrometer. The HPLC determination is conducted by using an Agilent 1200 DAD high pressure liquid chromatograph (Sunfire C18 150*4.6 mm chromatographic column) and a Waters 2695-2996 high pressure liquid chromatograph (Gimini C18 150*4.6 mm chromatographic column).
A Yantai Yellow Sea HSGF254 or Qingdao GF254 silica gel plate is adopted as a thin layer chromatography (TLC) silica gel plate. The specification adopted by the TLC is 0.15-0.20 mm, and the specification adopted by the thin layer chromatography for product separation and purification is 0.4-0.5 mm. The Yantai Yellow Sea silica gel of 200-300 mesh is generally utilized as a carrier in column chromatography.
Starting materials in the examples of the present invention are known and commercially available, or may be synthesized by using or according to methods known in the art.
Unless otherwise stated, all reactions of the present invention are carried out under a dry nitrogen or argon atmosphere with continuous magnetic stirring, the solvent is a dry solvent, and the reaction temperature is in degree centigrade (° C.).
N,N′-(2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(4-cyclopropyl-5-formylpicolinamide) [prepared according to the synthesis method in WO2021008491A1] (200 mg, 0.358 mmol) was placed in DMF (5 mL), followed by addition of methyl 4-aminobicyclo[2.2.2]octane-1-carboxylate (656.1 mg, 3.58 mmol) and HOAc (1.5 mL). The mixture was stirred at r oom temperature for 1 hr. Sodium cyanoborohydride (225.0 mg, 3.58 mmol) was added, and the mixture was stirred overnight at room temperature. After the reaction was compl eted, the residue was separated by reversed-phase column chromatography to obtain methyl 4-(((4-cyclopropyl-6-((3′-(4-cyclopropyl-5-(((4-(methoxycarbonyl)bicyclo[2.2.2]octan-1-yl)amino)methyl) picolinamido)-2,2′-dimethyl-[1,1′-biphenyl]-3-yl)carbamoyl)pyridin-3-yl)methyl)amino)bicyclo[2.2.2]octane-1-carboxylate (180 mg, 0.20 mmol, yield: 56.3%). MS m/z (ES I): 893.6 [M+H]+.
Methyl 4-(((4-cyclopropyl-6-((3′-(4-cyclopropyl-5-(((4-(methoxycarbonyl)bicyclo[2.2.2]octan-1-yl)amino)methyl) picolinamido)-2,2′-dimethyl-[1,1′-biphenyl]-3-yl)carbamoyl)pyridin-3-yl)methyl)amino)bicyclo[2.2.2]octane-1-carboxylate (350 mg, 0.39 mmol) was placed in a mixture solution of MeOH (5 mL), THF (5 mL) and water (5 mL), followed by addition of NaOH (314 mg, 7.84 mmol). The mixture was heated to 50° C. and stirred overnight under nitrogen protection. After the reaction was completed, the reaction solution was adjusted to pH of ˜6 with formic acid and then concentrated. The crude product was separated by reversed-phase column chromatography to obtain 4-(((6-((3′-(5-(((4-carboxybicyclo[2.2.2]octan-1-yl)amino) methyl)-4-cyclopropylpicolinamido)-2,2′-dimethyl-[1,1′-biphenyl]-3-yl)carbamoyl)-4-cyclopropylpyridin-3-yl)methyl)amino)bicyclo[2.2.2]octane-1-carboxylic acid (180 mg, 0.187 mmol, yield: 47.8%). MS m/z (ESI): 865.6 [M+H]+.
1H NMR (400 MHz, DMSO-d6) δ 10.31 (s, 2H), 8.55 (s, 2H), 7.89 (d, J=8.0 Hz, 2H), 7.57 (s, 2H), 7.31 (t, J=7.8 Hz, 2H), 6.97 (d, J=7.5 Hz, 2H), 3.87 (s, 4H), 2.28 (td, J=8.3, 4.2 Hz, 2H), 2.00 (s, 6H), 1.77 (dd, J=10.5, 5.3 Hz, 12H), 1.60 (dd, J=10.3, 5.5 Hz, 12H), 1.12 (dt, J=7.7, 4.9 Hz, 4H), 0.84 (q, J=5.2 Hz, 4H).
Dimethyl bicyclo[2.2.1]heptane-1,4-dicarboxylate (2.0 g, 9.42 mmol) was dissolved in THF (40 mL), and a solution of NaOH (377 mg, 9.42 mmol) in methanol (8 mL) was added dropwise. The mixture was stirred overnight at room temperature. After the reaction was completed, the reaction solution was carefully concentrated until the solvent was completely removed. The solid was washed with petroleum ether and filtered, and the filter cake was dissolved in water (50 mL). The aqueous solution was acidified with 2 M hydrochloric acid until the pH value was ˜4 and then extracted with ethyl acetate. The organic phase was washed with water and saturated brine, dried over anhydrous sodium sulfate and filtered, and the filtrate was concentrated to obtain 4-(methoxycarbonyl)bicyclo[2.2.1]heptane-1-carboxylic acid (1.49 g, yield: 80.0%).
4-(methoxycarbonyl)bicyclo[2.2.1]heptane-1-carboxylic acid (700 mg, 3.53 mmol) was dissolved in toluene (30 mL), followed by addition of diphenylphosphoryl azide (0.91 mL, 4.24 mmol) and triethylamine (0.98 mL, 7.06 mmol). The mixture was heated to 120° C. and reacted for 2 hrs under nitrogen protection. The reaction solution was cooled to room temperature, and then benzyl alcohol (0.73 mL, 7.06 mmol) was added. The mixture was reacted at 100° C. for 18 hrs under nitrogen protection. After the reaction was completed, the reaction solution was concentrated, and the crude product was separated by column chromatography to obtain methyl 4-(((benzyloxy)carbonyl)amino)bicyclo[2.2.1]heptane-1-carboxylate (1000 mg, 3.30 mmol, yield: 93.4%). MS m/z (ESI): 304.0 [M+H]+.
Methyl 4-(((benzyloxy)carbonyl)amino)bicyclo[2.2.1]heptane-1-carboxylate (1000 mg, 3.30 mmol) was dissolved in methanol (30 mL), followed by addition of 10% Pd/C (100 mg). The mixture was stirred overnight at room temperature under atmospheric hydrogen atmosphere. After the reaction was completed, the reaction solution was filtered, and the filtrate was concentrated to obtain methyl 4-aminobicyclo[2.2.1]heptane-1-carboxylate (500 mg, 2.955 mmol, yield: 89.6%). MS m/z (ESI): 170.0 [M+H]+.
N,N′-(2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(4-cyclopropyl-5-formylpicolinamide) (200 mg, 0.358 mmol) was dissolved in DMF (5 mL), followed by addition of methyl 4-aminobicyclo[2.2.1]heptane-1-carboxylate (484.7 mg, 2.864 mmol) and HOAc (1.5 mL). The mixture was stirred at room temperature for 60 min, and then NaBH3CN (225.0 mg, 3.58 mmol) was added. The resulting mixture was stirred overnight at room temperature under nitrogen protection. After the reaction was completed, the reaction solution was separated by column chromatography (water/acetonitrile/formic acid) to obtain dimethyl 4,4′-((((((2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(azanediyl))bis(carbonyl)) bis(4-cyclopropylpyridine-6,3-diyl))bis(methylene))bis(azanediyl))bis(bicyclo[2.2.1]heptane-1-carboxylate) (230 mg, 0.266 mmol, yield: 74.3%). MS m/z (ESI): 865.4 [M+H]+.
Dimethyl 4,4′-((((((2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(azanediyl))bis(carbonyl)) bis(4-cyclopropylpyridine-6,3-diyl))bis(methylene))bis(azanediyl))bis(bicyclo[2.2.1]heptane-1-carboxylate) (230 mg, 0.266 mmol) was added to a mixture solution of MeOH (5 mL), THF (5 mL) and water (5 mL), followed by addition of NaOH (212.7 mg, 5.317 mmol). The mixture was heated to 50° C. and stirred for 1 hr under nitrogen protection. After the reaction was completed, the reaction solution was adjusted to pH of ˜6 with formic acid and then concentrated. The crude product was separated by reversed-phase column chromatography to obtain 4,4′-((((((2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(azanediyl))bis(carbonyl)) bis(4-cyclopropylpyridine-6,3-diyl))bis(methylene))bis(azanediyl))bis(bicyclo[2.2.1]heptane-1-carboxylic acid) (160 mg, 0.181 mmol, yield: 67.9%). MS m/z (ESI): 837.4 [M+H]+.
1H NMR (400 MHz, DMSO-d6) δ 10.33 (s, 2H), 8.69 (s, 2H), 7.86 (d, J=8.2 Hz, 2H), 7.63 (s, 2H), 7.32 (t, J=7.8 Hz, 2H), 6.99 (dd, J=7.6, 1.3 Hz, 2H), 4.38-4.02 (m, 4H), 2.31-2.24 (m, 2H), 2.12-1.46 (m, 26H), 1.15 (d, J=7.9 Hz, 4H), 0.95-0.78 (m, 4H).
4,4′-((((((2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(azanediyl))bis(carbonyl))bis(4-cyclopropylpyridine-6,3-diyl))bis(methylene)) bis(azanediyl))bis(bicyclo[2.2.1]heptane-1-carboxylic acid) (50 mg, 0.060 mmol) was dissolved in DMF (3 mL), followed by addition of HOAc (1 mL) and aqueous formaldehyde solution (35%, 37.5 mg, 0.597 mmol). The mixture was stirred for 0.5 hrs at room temperature, and then NaBH3CN (37.5 mg, 0.597 mmol) was added. The resulting mixture was stirred overnight at room temperature under nitrogen protection. After the reaction was completed, the reaction solution was separated by reversed-phase column chromatography to obtain 4,4′-((((((2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(azanediyl)) bis(carbonyl))bis(4-cyclopropylpyridine-6,3-diyl))bis(methylene))bis(methylazanediyl))bis(bicyclo[2.2.1]heptane-1-carboxylic acid) (12 mg, 0.013 mmol, yield: 21.6%). MS m/z (ESI): 865.6 [M+H]+.
1H NMR (400 MHz, DMSO-d6) δ 10.30 (s, 2H), 8.52 (s, 2H), 7.90 (d, J=8.0 Hz, 2H), 7.59 (s, 2H), 7.31 (t, J=7.8 Hz, 2H), 6.97 (d, J=7.5 Hz, 2H), 3.81 (s, 4H), 2.40 (td, J=8.2, 4.2 Hz, 2H), 2.11 (s, 6H), 2.05-1.95 (m, 10H), 1.87-1.57 (m, 16H), 1.12 (d, J=8.1 Hz, 4H), 0.84 (d, J=5.2 Hz, 4H).
4-(methoxycarbonyl)bicyclo[2.2.1]heptane-1-carboxylic acid (800 mg, 4.04 mmol) was dissolved in dry THF (40 mL), and a BH3.Me2S/THF solution (5.25 mL, 5.25 mmol) was slowly added dropwise under an ice-water bath. After completion of the dropwise addition, the mixture was stirred overnight at room temperature. MeOH (10 mL) was added dropwise to the reaction solution, and the mixture was refluxed for 4 hrs to quench the reaction. The reaction solution was extracted with ethyl acetate and water, and the organic phase was washed with water and saturated brine, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated, and the crude product was separated by column chromatography to obtain methyl 4-(hydroxymethyl)bicyclo[2.2.1]heptane-1-carboxylate (600 mg, yield: 80.7%).
1H NMR (400 MHz, CDCl3) δ 3.70 (s, 2H), 3.67 (s, 3H), 2.06-1.93 (m, 2H), 1.73-1.62 (m, 4H), 1.58-1.54 (m, 2H), 1.43-1.34 (m, 2H).
Methyl 4-(hydroxymethyl)bicyclo[2.2.1]heptane-1-carboxylate (600 mg, 3.27 mmol) was dissolved in CH2Cl2 (20 mL), followed by addition of Dess-Martin reagent (1.66 g, 3.91 mmol). The mixture was stirred at room temperature, and the reaction was monitored by TLC until the starting materials were consumed. The reaction solution was filtered through diatomaceous earth, and the filtrate was concentrated and separated by column chromatography to obtain methyl 4-formylbicyclo[2.2.1]heptane-1-carboxylate (500 mg, yield: 84.3%).
1H NMR (400 MHz, CDCl3) δ 9.81 (s, 1H), 3.70 (s, 3H), 2.13-2.00 (m, 4H), 1.86-1.82 (m, 2H), 1.79-1.68 (m, 2H), 1.60-1.52 (m, 2H).
Methyl 4-formylbicyclo[2.2.1]heptane-1-carboxylate (200 mg, 1.10 mmol) and benzylamine (235 mg, 2.20 mmol) were added to a mixture solution of MeOH (20 mL) and HOAc (0.2 mL), and the mixture was stirred at room temperature for 30 min under nitrogen protection, followed by addition of NaBH(OAc)3 (465 mg, 2.20 mmol). The resulting mixture was stirred overnight at room temperature. After the reaction was completed, saturated aqueous NaHCO3 solution was added, and the reaction solution was extracted with CH2Cl2. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated, and the crude product was separated by column chromatography to obtain methyl 4-((benzylamino)methyl)bicyclo[2.2.1]heptane-1-carboxylate (300 mg, yield: 100%). MS m/z (ESI): 274.2 [M+H]+.
Methyl 4-((benzylamino)methyl)bicyclo[2.2.1]heptane-1-carboxylate (300 mg, 1.10 mmol) and 10% Pd/C (50 mg) were placed in methanol (30 mL), and the mixture was stirred overnight at room temperature under atmospheric hydrogen atmosphere. The reaction solution was filtered, and the filtrate was concentrated to obtain methyl 4-(aminomethyl)bicyclo[2.2.1]heptane-1-carboxylate (194 mg, yield: 96.5%). MS m/z (ESI): 184.1 [M+H]+.
N,N′-(2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(4-cyclopropyl-5-formylpicolinamide) (115 mg, 0.206 mmol) and methyl 4-(aminomethyl)bicyclo[2.2.1]heptane-1-carboxylate (189 mg, 1.03 mmol) were placed in a mixture solution of DMF (3 mL) and HOAc (0.75 mL) and the mixture was stirred at room temperature for 1 hr under nitrogen protection, followed by addition of NaBH3CN (65 mg, 1.03 mmol). The resulting mixture was stirred overnight at room temperature. After the reaction was completed, the reaction mixture was separated by reversed-phase column chromatography to obtain methyl 4-({[(4-cyclopropyl-6-{[3-(3-{4-cyclopropyl-5-[({[4-(methoxycarbonyl) bicyclo[2.2.1]heptan-1-yl]methyl}amino)methyl]pyridin-2-amido}-2-methylphenyl)-2-methylphenyl]carbamoyl}pyridin-3-yl)methyl]amino}methyl) bicyclo[2.2.1]heptane-1-carboxylate (124 mg, yield: 67.5%). MS m/z (ESI): 893.5 [M+H]+.
Methyl 4-({[(4-cyclopropyl-6-{[3-(3-{4-cyclopropyl-5-[({[4-(methoxycarbonyl) bicycle[2.2.1]heptan-1-yl]methyl}amino)methyl]pyridin-2-amido}-2-methylphenyl)-2-methylphenyl]carbamoyl}pyridin-3-yl) methyl]amino}methyl)bicyclo[2.2.1]heptane-1-carboxylate (124 mg, 0.139 mmol) was placed in a mixture solution of MeOH (3 mL), THF (3 mL) and water (1.5 mL), followed by addition of NaOH (83 mg, 2.08 mmol). The mixture was heated to 50° C. and stirred for 1 hr under nitrogen protection. After the reaction was completed, the reaction solution was adjusted to pH of ˜6 with formic acid and then concentrated, and the crude product was separated by reversed-phase column chromatography to obtain 4,4′-(((((((2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(azanediyl))bis(carbonyl))bis(4-cyclopropylpyridine-6,3-diyl)) bis(methylene))bis(azanediyl))bis(methylene))bis(bicyclo[2.2.1]heptane-1-carboxylic acid) formate (45.5 mg, yield: 34.1%). MS m/z (ESI): 865.5 [M+H]+.
1H NMR (400 MHz, DMSO-d6) δ 10.30 (s, 2H), 8.57 (s, 2H), 7.88 (d, J=8.0 Hz, 2H), 7.60 (s, 2H), 7.31 (t, J=7.8 Hz, 2H), 6.98 (m, 2H), 3.98 (s, 4H), 2.67 (s, 4H), 2.28 (m, 2H), 2.01 (m, 6H), 1.90-1.82 (m, 4H), 1.65-1.58 (m, 4H), 1.54 (t, J=10.3 Hz, 4H), 1.45 (m, 4H), 1.32 (m, 4H), 1.15-1.09 (m, 4H), 0.88-0.82 (m, 4H).
Methyl 4-(2-oxoethyl)bicyclo[2.2.1]heptane-1-carboxylate (350 mg, 1.78 mmol) and benzylamine (382 mg, 3.57 mmol) were placed in a mixture solution of MeOH (20 mL) and HOAc (0.2 mL), and the mixture was stirred at room temperature for 30 min under nitrogen protection, followed by addition of NaBH(OAc)3 (756 mg, 3.57 mmol). The resulting mixture was stirred overnight at room temperature. After the reaction was completed, saturated aqueous NaHCO3 solution was added, and the reaction solution was extracted with CH2Cl2. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated, and the residue was separated by column chromatography to obtain methyl 4-(2-(benzylamino)ethyl)bicyclo[2.2.1]heptane-1-carboxylate (425 mg, yield: 82.9%). MS m/z (ESI): 288.2 [M+H]+.
Methyl 4-(2-(benzylamino)ethyl)bicyclo[2.2.1]heptane-1-carboxylate (425 mg, 1.48 mmol) and 10% Pd/C (50 mg) were placed in methanol (30 mL), and the mixture was stirred overnight at room temperature under atmospheric hydrogen atmosphere. After the reaction was completed, the reaction solution was filtered, and the filtrate was concentrated to obtain methyl 4-(2-aminoethyl) bicyclo[2.2.1]heptane-1-carboxylate (246 mg, yield: 84.3%). MS m/z (ESI): 198.1 [M+H]+.
N,N′-(2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(4-cyclopropyl-5-formylpicolinamide) (110 mg, 0.197 mmol) and methyl 4-(2-aminoethyl)bicyclo[2.2.1]heptane-1-carboxylate (233 mg, 1.18 mmol) were placed in a mixture solution of DMF (3 mL) and HOAc (0.75 mL), and the mixture was stirred at room temperature for 1 hr under nitrogen protection, followed by addition of NaBH3CN (74 mg, 1.18 mmol). The resulting mixture was stirred overnight at room temperature. After the reaction was completed, the reaction mixture was separated by reversed-phase column chromatography to obtain dimethyl 4,4′-(((((((2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(azanediyl))bis(carbonyl)) bis(4-cyclopropylpyridine-6,3-diyl))bis(methylene))bis(azanediyl))bis(ethane-2,1-diyl))bis(bicyclo[2.2.1]heptane-1-carboxylate) (100 mg, yield: 55.1%). MS m/z (ESI): 921.5 [M+H]+.
Dimethyl 4,4′-(((((((2,2′-dimethyl-[1,1′-biphenyl]-3,3′-diyl)bis(azanediyl))bis(carbonyl)) bis(4-cyclopropylpyridine-6,3-diyl))bis(methylene))bis(azanediyl))bis(ethane-2,1-diyl)) bis(bicyclo[2.2.1]heptane-1-carboxylate) (100 mg, 0.108 mmol) was placed in a mixture solution of methanol (3 mL), THF (3 mL) and water (1.5 mL), followed by addition of NaOH (90 mg, 2.25 mmol). The mixture was heated to 50° C. and stirred for 1 hr. After the reaction was completed, the reaction solution was adjusted to pH of ˜6 with formic acid and then concentrated, and the residue was separated by reversed-phase column chromatography to obtain 4-((((6-((3′-(5-(((4-carboxybicyclo[2.2.1]heptyl-1-yl) ethyl)aminomethyl)-4-cyclopropylmethylpyridinamido)-2,2′-dimethyl-[1,1′-biphenyl]-3-yl)carbamoyl)-4-cyclopropylpyridin-3-yl)methyl)amino) ethyl)bicyclo[2.2.1]heptyl-1-carboxylic acid (45.5 mg, yield: 34.1%). MS m/z (ESI): 893.5 [M+H]+.
1H NMR (400 MHz, DMSO-d6) δ 10.30 (s, 2H), 8.55 (s, 2H), 7.88 (m, 2H), 7.58 (s, 2H), 7.31 (t, J=7.8 Hz, 2H), 6.98 (m, 2H), 3.96 (s, 4H), 2.63 (t, J=7.8 Hz, 4H), 2.29 (m, 2H), 2.00 (s, 6H), 1.86 (m, 4H), 1.69 (t, J=7.8 Hz, 4H), 1.56-1.30 (m, 16H), 1.16-1.10 (m, 4H), 0.87-0.82 (m, 4H).
The effect of compounds of the examples of the present invention on the PD-1/PD-L1 interaction was determined by the PD-1/PD-L1 binding assay kit from Cisbio (#64ICP01PEG or 64ICP01PEH). The detailed experimental process was as follows:
The effect of compounds of the examples of the present invention on the PD-1/PD-L1 interaction expressed on cell surfaces and the related influence on T cell functions were determined by a Jurkat reporter gene cellular assay. Briefly, the reporter gene plasmid of NF-κB-luc and the plasmid of human PD-1 were transfected into Jurkat cells to establish a stably transfected cell line capable of stably expressing both PD-1 and NF-κB-Luc reporter genes; the expression level of PD-1 on the cell surface was confirmed by flow cytometry; and the expression of the reporter gene was confirmed via the response of the reporter gene stimulated by OKT-3 and Raji cells.
In addition, the plasmid of human PD-L1 was transfected into Raji cells to obtain a cell line capable of stably expressing PD-L1. Jurkat/NF-κB-luc/PD1 cells and Raji-PD-L1 cells were then cocultured and stimulated with OKT-3. On this basis, the compounds were added, and the enhancement of the signal pathway of T cell activation by the inhibitory effect of the compounds on the PD-1/PD-L1 interaction was evaluated by readings of reporter gene responses. The specific experimental process was as follows:
The bioactivity data of the compounds of the specific examples indicate that the series of compounds of the present invention have a strong inhibitory effect on the PD-1/PD-L1 interaction, and moreover, such an inhibitory effect can enhance or recover the activation of T cells at the cellular level.
In this experiment, Caco-2 cells adopted were human colon cancer cells. Similar to normal intestinal epithelium, the Caco-2 cells were able to form differentiated monolayer cells with a plurality of characteristics under proper culture conditions, such as tight connection, expression of microvilli and brush border enzyme, and the like. The permeability of compounds on a Caco-2 cell model is closely related to the absorption in a human body, and the Caco-2 cell model is widely applied to the evaluation of the permeability and the active transport process of compounds in the intestinal tract. The permeability and the efflux ratio of the compounds of the examples of the present invention and the comparative compounds were evaluated using a Caco-2 cell model in this experiment. The specific process was as follows:
Working solutions were obtained by diluting with Hank's balanced salt solution (HBSS, Invitrogen) containing 25 mM HEPES (pH 7.4). The detection concentration of the test compound was 10 μM. This study was a two-way permeation experiment from the apical side to the basolateral side (A−B) and from the basolateral side to the apical side (B−A), and a 90-minute incubation at 37° C. was required. After the incubation, the samples diluted with buffer were detected by LC-MS/MS. The concentrations of the compounds were quantified using a standard curve.
Wherein, “VA” refers to the volume in the acceptor pore, “Area” refers to the surface area of the membrane, and “time” is the total transport time in seconds.
The data of the membrane permeability test in Table 2 show that the compounds of the examples of the present invention have significantly improved A−B membrane permeability due to modification with the linker groups at the two ends of the compounds, while the comparative compounds 2, 4, and 6 all have low A−B Papp value and poor membrane permeability although they also have a symmetric diacid structure. In addition, through in vivo pharmacokinetic experiments of mice, the inventors further found that these compounds were hardly absorbed if directly administered orally and oral administration was possible only by administering their prodrugs (comparative compounds 1, 3 and 5), and these compounds were different from the compounds of the examples of the present invention in metabolic pathway.
The purpose of this study is to study the pharmacokinetic behaviors of some compounds of the present invention, and administration routes were: single per oral administration (PO) or intravenous injection (IV) to ICR mice or rats.
The compounds used in this test were compounds of specific examples of the present invention and the comparative compounds listed.
ICR mice male N=9 original source: Shanghai Sippr-BK Laboratory Animal Co. Ltd.
ICR rats male N=3 original source: Shanghai Sippr-BK Laboratory Animal Co. Ltd.
The compounds were each dissolved in a corresponding vehicle, mixed well by shaking and subjected to ultrasonic treatment to obtain colorless clear solutions. 9 mice and 3 rats were orally administered with the solution after fasting overnight. The mice were dosed at 10 mg/kg (or 2 mg/kg by IV) and the rats were dosed at 5 mg/kg (or 1 mg/kg by IV).
With about 90 μL/time point, blood was drawn from the submaxillary vein, and heparin sodium was added for anticoagulation. The blood samples were placed onto the ice and centrifuged (centrifugation conditions: 8000 rpm, 6 min, 2-8° C.) within 1 hr to isolate the plasmas. The time points for blood sampling were at 0 hrs, 0.25 hrs, 0.5 hrs, 1 hr, 2 hrs, 4 hrs, 6 hrs, 8 hrs and 24 hrs after administration. The samples were stored in a refrigerator at −20° C.
40 μL of plasma sample was added to 160 μL of cold acetonitrile containing an internal standard, and the mixture was vortexed for 3 min and centrifuged at 11,000 rpm for 5 min. 100 μL of the supernate was added to 100 μL of water, and 5 μL of the mixed solution was loaded for LC-MS/MS analysis. For comparative compounds 1, 3, 5, the prodrug compounds and corresponding prototype compounds 2, 4, 6 were all analyzed, and specific analysis results are shown in Table 4.
Experiments show that compared with comparative compounds, the compounds of the examples of the present invention have significantly improved oral exposure and good oral availability and are sufficient to support the development of oral administration agents in the next step.
Experiments show that compared with comparative compounds, the compounds of the examples of the present invention have significantly improved oral exposure and good oral availability and are sufficient to support the development of oral administration agents in the next step.
All documents mentioned in the present invention are incorporated as references, just as each document is individually cited as a reference. In addition, it should be understood that various modifications or changes may be made by those skilled in the art after reading the above disclosure of the present invention, and these equivalent forms also fall within the scope defined by the claims appended hereto.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202210216572.8 | Mar 2022 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CN2023/079912 | 3/6/2023 | WO |
