HOMOGENEOUS MULTIPLEX PCR BY FLUORESCENCE AND TM

Information

  • Research Project
  • 6498790
  • ApplicationId
    6498790
  • Core Project Number
    R42GM058983
  • Full Project Number
    5R42GM058983-03
  • Serial Number
    58983
  • FOA Number
  • Sub Project Id
  • Project Start Date
    5/1/1999 - 25 years ago
  • Project End Date
    1/31/2003 - 21 years ago
  • Program Officer Name
    SWAIN, AMY L
  • Budget Start Date
    2/1/2002 - 22 years ago
  • Budget End Date
    1/31/2003 - 21 years ago
  • Fiscal Year
    2002
  • Support Year
    3
  • Suffix
  • Award Notice Date
    1/24/2002 - 23 years ago
Organizations

HOMOGENEOUS MULTIPLEX PCR BY FLUORESCENCE AND TM

DESCRIPTION (applicant's abstract): Our long-term goal is to advance techniques and instrumentation for rapid, automated analysis of nucleic acids during amplification. Our approach is to add fluorescent probes before amplification and analyze the results in real-time, completing the entire process in 10-20 min. No additional sample processing, membranes, arrays, or gels are necessary. In Phase I, we demonstrated the feasibility of a new solution multiplexing technique that uses both probe melting temperature (Tm) and color. In Phase II, our specific aims are to: 1. Prototype a flexible multi-spectral rapid thermal cycler that can monitor any visible fluorescent dye or combination of fluorescent dyes. Design and produce a small, inexpensive, multi-spectral rapid thermal cycler for the commercial market. 2. Provide software to select hybridization probes for multiplex analysis at highly polymorphic loci based on probe Tm. 3. Demonstrate multiplex analysis with "virtual" 2-dimensional arrays of color and Tm. Instead of physical separation, probe color and Tm are used to identify products. Human HLA loci will serve as highly polymorphic targets. When both probe color and Tm are used to identify products, high-order multiplexing can be performed in solution. PROPOSED COMMERCIAL APPLICATION: We propose a small footprint, rapid, inexpensive fluorescent thermal cycler with continuously variable excitation and emission wavelengths. This will be the first real-time instrument compatible with all visible/near IR fluorescent probes and amplification techniques. Multiplexing by fluorescence and Tm greatly expands the power of real-time nucleic acid analysis.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R42
  • Administering IC
    GM
  • Application Type
    5
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    230444
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
    NIGMS:230444\
  • Funding Mechanism
  • Study Section
    ZRG1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    IDAHO TECHNOLOGY
  • Organization Department
  • Organization DUNS
  • Organization City
    SALT LAKE CITY
  • Organization State
    UT
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    84108
  • Organization District
    UNITED STATES