Claims
- 1. An isolated antigen having a molecular weight of from about 60 to 100 Kd, said antigen comprising a 44 amino acid epitope, said epitope having the amino acid sequence of SEQ. I.D. No. 4 and wherein said antigen is capable of binding 3E10 antibody.
- 2. An isolated antigen according to claim 1 wherein said antigen has the amino acid sequence of SEQ. I.D. No. 6.
- 3. A method for producing an antigen comprising the steps of:
- providing a microorganism comprising a DNA sequence which, upon culturing, expresses an antigen having a molecular weight of from about 60 to 100 Kd, said antigen comprising a 44 amino acid epitope, said epitope having the amino acid sequence of SEQ. I.D. No. 4 and wherein said antigen is capable of binding 3E10 antibody;
- culturing said microorganism in a nutrient medium for a sufficient length of time and under suitable conditions to form a mature cell culture which has expressed a recoverable amount of said antigen;
- recovering said antigen from said mature cell culture to provide said antigen in a purified form.
- 4. A method for producing an antigen according to claim 3 wherein said antigen has the amino acid sequence of SEQ. I.D. No. 6.
Parent Case Info
This application is a continuation-in-part application of U.S. Ser. No. 08/316,068, filed Sep. 30, 1994, now abaondened, which is in turn a continuation application of U.S. Ser. No. 08/033,120, filed Mar. 18, 1993, now abandoned, the entire contents of each of which are hereby incorporated by reference herein.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4812397 |
Weisbart |
Mar 1989 |
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Foreign Referenced Citations (1)
Number |
Date |
Country |
8909273 |
Oct 1989 |
WOX |
Continuations (1)
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Number |
Date |
Country |
Parent |
33120 |
Mar 1993 |
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Continuation in Parts (1)
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Number |
Date |
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Parent |
316068 |
Sep 1994 |
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