Claims
- 1. A method for producing a transgenic mouse wherein the germ cells comprise at least one inactivated endogenous immunoglobulin heavy chain locus in which all of the J segment genes are deleted to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged locus and the expression of an endogenous immunoglobulin heavy chain from the inactivated locus, said method comprising the steps of:
- (a) deleting the J segment genes from at least one endogenous heavy chain locus in a mouse embryonic stem cell to prevent rearrangement of said locus and to prevent formation of a transcript of a rearranged immunoglobulin heavy chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker; and
- (b) producing from the embryonic stem cell a transgenic mouse whose germ cells comprise at least one immunoglobulin heavy chain locus in which all of the J segment genes are deleted.
- 2. A method for producing a transgenic mouse and its progeny, wherein the somatic and germ cells comprise at least one inactivated endogenous immunoglobulin heavy chain locus in which all of the J segment genes are deleted to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged locus and the expression of an endogenous immunoglobulin heavy chain from the inactivated locus, said method comprising the steps of:
- (a) deleting the J segment genes from at least one endogenous heavy chain locus in a mouse embryonic stem cell to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin heavy chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker;
- (b) producing from the embryonic stem cell a transgenic mouse whose germ cells comprise at least one immunoglobulin heavy chain locus in which all of the J segment genes are deleted, and
- (c) breeding the transgenic mouse as needed to produce a transgenic mouse and its progeny whose somatic and germ cells comprise at least one inactivated endogenous immunoglobulin heavy chain locus in which all of the J segment genes are deleted.
- 3. The method according to claim 1 or claim 2, wherein the gene encoding a selectable marker is a neomycin resistance gene.
RELATED U.S. APPLICATION DATA
This application is a divisional of United States application Ser. No. 08/430,938, filed Apr. 27, 1995, now abandoned which is a continuation-in-part of United States application Ser. No. 08/234,145, filed Apr. 28, 1994, now abandoned, which is a continuation-in-part of United States application Ser. No. 08/112,848, filed Aug. 27, 1993, now abandoned, which is a continuation-in-part of United States application Ser. No. 07/919,297, filed Jul. 24, 1992, now abandoned, which is a continuation-in-part of United States application Ser. No. 07/610,515, filed Nov. 8, 1990, now abandoned, which is a continuation-in-part of United States application Ser. No. 07/466,008, filed Jan. 12, 1990, now abandoned.
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Divisions (1)
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Number |
Date |
Country |
Parent |
430938 |
Apr 1995 |
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Continuation in Parts (5)
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Number |
Date |
Country |
Parent |
234145 |
Apr 1994 |
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Parent |
112848 |
Aug 1993 |
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Parent |
919297 |
Jul 1992 |
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Parent |
610515 |
Nov 1990 |
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Parent |
466008 |
Jan 1990 |
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