Claims
- 1. An isolated and purified nucleic acid, said nucleic acid comprising nucleotides which code for the amino acid sequence of SEQ ID NO: 2.
- 2. An isolated and purified nucleic acid which codes for human diacylglycerol kinase ι, said nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1.
- 3. A recombinant vector comprising the nucleic acid molecule of claim 2.
- 4. The recombinant vector of claim 3, wherein said recombinant vector is a plasmid.
- 5. The recombinant vector of claim 3, wherein said recombinant vector is a prokaryotic or eukaryotic expression vector.
- 6. The recombinant vector of claim 3, wherein the nucleic acid molecule is operably linked to a heterologous promoter.
- 7. A host cell comprising the nucleic acid of claim 2.
- 8. The host cell of claim 7, wherein the host cell is a eukaryotic host cell.
- 9. The host cell of claim 7, wherein the host cell is a prokaryotic host cell.
- 10. An in vitro method of decreasing intracellular levels of diacylglycerol and increasing intracellular levels of phosphatidic acid comprising introducing into a eukaryotic cell a nucleic acid, said nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1.
- 11. An isolated and purified nucleic acid sequence whose complement hybridizes to SEQ ID NO: 1 under the conditions of 65° C. overnight in a solution comprising 5×SSPE, 5×Denhardt's, 0.2% SDS, and 0.1% Na2P4O7, followed by washing twice in 0.6×SSPE, 0.1% SDS, and 0.1% Na2P4O7 at 65° C. for 30 minutes, wherein said nucleic acid sequence codes for a protein having diacylglycerol kinase enzymatic activity.
- 12. The nucleic acid sequence defined in claim 11, wherein said nucleic acid sequence is subcloned into a plasmid.
- 13. The nucleic acid sequence defined in claim 11, wherein said nucleic acid sequence is subcloned into a prokaryotic or eukaryotic expression vector.
- 14. The nucleic acid sequence defined in claim 11, wherein said nucleic acid sequence is stably or transiently incorporated into a prokaryotic or eukaryotic host cell.
- 15. An in vitro method of decreasing intracellular levels of diacylglycerol and increasing intracellular levels of phosphatidic acid comprising introducing into a eukaryotic cell the nucleic acid sequence of claim 11.
RELATED APPLICATIONS
This application is related to and claims the benefit of United States Provisional Application Ser. No. 60/103,079 of Steven M. Prescott, Li Ding, Elie Traer, Thomas McIntyre, and Guy A. Zimmerman filed Oct. 5, 1998 and entitled “Cloning and Characterization of a Novel Diacylglycerol Kinase, DGKι,” which is incorporated herein by this reference.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5976875 |
Prescott et al. |
Nov 1999 |
|
Non-Patent Literature Citations (2)
Entry |
Ding et al., “The Cloning and Characterization of a Novel Human Diacylglycerol Kinase, DGKI”; Journal of Biological Chemistry; vol. 273; pp. 32746-32752, 1998. |
Masai et al., “DROSOPHILA Retinal Degeneration A Gene Encodes an Eye-Specific Diacylglycerol Kinase with Cysteine-Rich Zinc-Finger MOTIFS and Ankyrin Repeats”; Proc. Natl. Acad.; vol. 90; pp. 11157-11161, 1993. |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/103079 |
Oct 1998 |
US |