Claims
- 1. An isolated polypeptide, comprising an amino acid sequence selected from the group consisting of:
a) the amino acid sequence of SEQ I) NO:2; b) amino acid residues 1 to 198 of SEQ ID NO:2; c) amino acid residues 25 to 198 of SEQ ID NO:2; d) the amino acid sequence of SEQ ID NO:5; e) amino acid residues 1 to 180 of SEQ ID NO:5; and f) amino acid residues 25 to 180 of SEQ ID NO:5.
- 2. An isolated nucleic acid molecule, wherein the nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:6, (b) a nucleic acid molecule encoding the amino acid sequence of SEQ ID NO:5, and (c) a nucleic acid molecule that remains hybridized following stringent wash conditions to a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of: nucleotides 88 to 540 of SEQ ID NO:4, nucleotides 1 to 540 of SEQ ID NO:4, or the complement thereof.
- 3. The isolated nucleic acid molecule of claim 2, wherein any difference between the amino acid sequence encoded by the nucleic acid molecule and the corresponding amino acid sequence of SEQ ID NO:2, is due to a conservative amino acid substitution.
- 4. The isolated nucleic acid molecule of claim 2, comprising the nucleotide sequence of SEQ ID NO:1.
- 5. The isolated nucleic acid molecule of claim 2, wherein the nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of: nucleotides 88 to 594 of SEQ ID NO:1, or nucleotides 88 to 540 of SEQ ID NO:4.
- 6. A vector, comprising the isolated nucleic acid molecule of claim 5.
- 7. An expression vector, comprising the isolated nucleic acid molecule of claim 5, a transcription promoter, and a transcription terminator, wherein the promoter is operably linked with the nucleic acid molecule, and wherein the nucleic acid molecule is operably linked with the transcription terminator.
- 8. The expression vector of claim 7 further encoding an affinity tag.
- 9. A recombinant host cell comprising the expression vector of claim 7, wherein the host cell is selected from the group consisting of bacterium, yeast cell, fungal cell, insect cell, mammalian cell, avian, and plant cell.
- 10. A method of using the expression vector of claim 7 to produce Zinf2 protein, comprising culturing recombinant host cells that comprise the expression vector and that produce the Zinf2 protein.
- 11. The protein produced by the method of claim 10.
- 12. The method of claim 10, further comprising isolating the Zinf2 protein from the cultured recombinant host cells.
- 13. An antibody or antibody fragment that specifically binds with the polypeptide of claim 1.
- 14. The antibody of claim 13, wherein the antibody is selected from the group consisting of: (a) polyclonal antibody, (b) murine monoclonal antibody, (c) humanized antibody derived from (b), and (d) human monoclonal antibody.
- 15. An anti-idiotype antibody that specifically binds with the antibody or antibody fragment of claim 13.
- 16. A method of detecting the presence of Zinf2 gene expression in a biological sample, comprising:
(a) contacting a Zinf2 nucleic acid probe under hybridizing conditions with either (i) test RNA molecules isolated from the biological sample, or (ii) nucleic acid molecules synthesized from the isolated RNA molecules, wherein the probe consists of a nucleotide sequence comprising a portion of the nucleotide sequence of the nucleic acid molecule of claim 5, or complements thereof, and (b) detecting the formation of hybrids of the nucleic acid probe and either the test RNA molecules or the synthesized nucleic acid molecules, wherein the presence of the hybrids indicates the presence of Zinf2 RNA in the biological sample, or, (a′) contacting the biological sample with an antibody, or an antibody fragment, of claim 13, wherein the contacting is performed under conditions that allow the binding of the antibody or antibody fragment to the biological sample, and (b′) detecting any of the bound antibody or bound antibody fragment.
- 17. An isolated polypeptide, comprising an amino acid sequence selected from the group consisting of: the amino acid sequence of amino acid residues 25 to 180 of SEQ ID NO:2, and the amino acid sequence of SEQ ID NO:2.
- 18. A composition, comprising a carrier and the polypeptide of claim 15.
Parent Case Info
[0001] This application is related to Provisional Application No. 60/198,889 filed on Apr. 14, 2000. Under 35 U.S.C. §119(e)(1), this application claims benefit of said Provisional Application.
Provisional Applications (1)
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Number |
Date |
Country |
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60198889 |
Apr 2000 |
US |