Claims
- 1. An isolated, polypeptide with an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of:
- a) a full length amino acid sequence of SEQ ID NO:2,
- b) an amino acid sequence having amino acids 49 to 113 of SEQ ID NO:2,
- c) an amino acid sequence having amino acids 231 to 486 of SEQ ID NO:2, and
- d) an amino acid sequence having amino acids 1 to 481 of SEQ ID NO:2.
- 2. The isolated, polypeptide of claim 1, wherein said polypeptide has 99% identity to said amino acid sequence.
- 3. The isolated, polypeptide of claim 2, wherein said polypeptide has 100% identity to said amino acid sequence.
- 4. The isolated, polypeptide of claim 2, wherein said polypeptide has the full length amino acid scquence of SEQ ID NO:2.
- 5. The isolatated, polypeptide of claim 2, wherein said polypeptide has amino acids 49 to 113 of SEQ ID NO:2.
- 6. The isolated, polypeptide of claim 2, wherein said polypeptide has amino acids 231 to 486 of SEQ ID NO:2.
- 7. The isolated, polypeptide of claim 2, wherein said polypeptide has amino acids 1 to 481 of SEQ ID NO:2.
- 8. An isolated, polypeptide comprising a polypeptide fragment from hPAK65 that binds rac1 or CDC42H.
- 9. The isolated, polypeptide of claim 8, wherein said polypeptide fragment has an amino acid sequence from SEQ ID NO:2 that spans amino acid 49 to amino acid 113.
- 10. The isolated, polypeptide of claim 8, wherein said polypeptide ae fragment binds an immunoglobulin.
- 11. An isolated, polypeptide comprising a polypeptide fragment from hPAK65 that corresponds to a p-21 binding domain.
- 12. The isolated, polypeptide of claim 11, wherein said polypeptide fragment has an amino acid sequence from SEQ ID NO:2 that spans amino acid 49 to amino acid 113.
- 13. An isolated, polypeptide comprising a polypeptide fragment from hPAK65 that corresponds to a kinase domain of hPAK65.
- 14. The isolated, polypeptide of claim 13, wherein said polypeptide fragment is from SEQ ID NO:2 and spans amino acid 1 to 481.
- 15. An isolated, polypeptide comprising a heterologous polypeptide joined to a second polypeptide with an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of:
- a) a full length amino acid sequence of SEQ ID NO.:2,
- b) an amino acid sequence having amino acids 49 to 113 of SEQ ID NO.:2,
- c) an amino acid sequence having amino acids 231 to 486 of SEQ ID NO.:2, and
- d) an amino acid sequence having amino acids 1 to 481 of SEQ ID NO.:2.
- 16. The isolated, polypeptide of clain 15, wherein said heterologous protein is glutathione-S-transferase.
- 17. The isolated, polypeptide of claim 15, wherein said heterologous protein is ubiquitin.
- 18. The isolated, polypeptide of claim 15, wherein said heterologous protein is a Myc epitope.
- 19. The isolated, polypeptide of claim 15, wherein said heterologous protein is a signal peptide.
- 20. An isolated, polypetide comprising a heterologous polypeptide joined to a second polypeptide from hPAK65 that binds rac1 or CDC42H and has an amino acid sequence from SEQ ID NO:2 Lhat spans amino acid 49 to amino acid 113.
- 21. The isolated, polypeptide of claim 20, wherein said heterologous protein is glutathione-S-transferase.
- 22. The isolated, polypeptide of claim 20, wherein said heterologous protein is a ubiquitin.
- 23. The isolated, polypeptide of claim 20, wherein said heterologous protein is a Myc epitope.
- 24. The isolated, polypeptide of claim 20, wherein said heterologous protein is a signal peptide.
- 25. An isolated, polypeptide comprising a heterologous polypeptide joined to a second polypeptide from hPAK65 that corresponds to a kinase domain of hPAK65 and has an amino acid sequence from SEQ ID NO:2 that spans amino acid 1 to amino acid 481.
- 26. The isolated, polypeptide of claim 25, wherein said heterologous protein is glutathione-S-transferase.
- 27. The isolated, polypeptide of claim 25, wherein said heterologous protein is ubiquitin.
- 28. The isolated, polypeptide of claim 25, wherein said heterologous protein is a Myc epitope.
- 29. The isolated, polypeptide of claim 25, wherein said heterologous protein is a signal peptide.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a Divisional of U.S. application Ser. No. 08/369,780, filed Jan. 6, 1995 now U.S. Pat. No. 5,518,911.
Non-Patent Literature Citations (4)
Entry |
Knaus et al. (1995) Science 269: 221-223. |
Leberer et al. (1992) EMBO J. 11(13): 4815-4824. |
Manser et al. (1992) J. Biol. Chem. 267(23): 16025-16028. |
Manser et al. (1995) Nature 367: 40-46. |
Continuations (1)
|
Number |
Date |
Country |
Parent |
369780 |
Jan 1995 |
|