Claims
- 1. A human parathyroid hormone (1-84) mutein comprising substitution of a cysteine residue for one amino acid residue within the region of amino acid residue number 34 to 47 in the amino acid sequence of human parathyroid hormone (1-84), wherein cleavage of the mutein at the cysteine residue produces an human parathyroid hormone fragment having parathyroid hormone activity.
- 2. The human parathyroid hormone (1-84) mutein claimed in claim 1, which is human parathyroid hormone (1-84).
- 3. A recombinant DNA having a nucleotide sequence coding for the human parathyroid hormone (1-84) mutein claimed in claim 1.
- 4. A vector containing the recombinant DNA claimed in claim 3.
- 5. A vector in which the recombinant DNA claimed in claim 3 is inserted into a region controlled by an Escherichia coli T7 promoter.
- 6. A host cell which is transformed by the recombinant DNA claimed in claim 3.
- 7. A process for producing a human parathyroid hormone (1-84) mutein which comprises cultivating the transformed host cell claimed in claim 6 in a culture medium under conditions suitable for expression of the mutein.
Priority Claims (2)
Number |
Date |
Country |
Kind |
3-198056 |
Aug 1991 |
JPX |
|
4-169713 |
Jun 1992 |
JPX |
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Parent Case Info
This application is a continuation of application Ser. No. 08/402,970, filed on Mar. 10, 1995, now abandoned, which is a continuation of application Ser No. 07/926,787, filed on Aug. 5, 1992 now abandoned.
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Entry |
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Continuations (2)
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Number |
Date |
Country |
Parent |
402970 |
Mar 1995 |
|
Parent |
926787 |
Aug 1992 |
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