Claims
- 1. A hybrid molecule comprising a first domain and a second domain covalently linked, wherein
(a) said first domain comprises a domain which is capable of specific binding to Gb3; (b) said second domain comprising a moiety selected from the group consisting of drug moiety, a nucleic acid, a probe, a polypeptide, and a hook, with the proviso that the second domain is not a verotoxin or a fragment thereof.
- 2. The hybrid molecule of claim 1, wherein the first domain is a verotoxin or a verotoxin subunit.
- 3. The hybrid molecule of claim 1, wherein the first domain is VT-B.
- 4. The hybrid molecule of claim 1, wherin the second domain is a polypeptide.
- 5. The hybrid molecule of claim 4, wherein the polypeptide is a DNA binding element.
- 6. The hybrid molecule of claim 1, wherein the second domain is a nucleic acid.
- 7. The hybrid molecule of claim 6, wherein the nucleic acid is an antisense nucleic acid.
- 8. A pharmaceutical composition comprising the hybrid molecule of claim 1 and a pharmaceutically acceptable carrier.
- 9. A method for modulating a cell-associated activity comprising contacting a cell with the hybrid molecule of claim 1 such that a cell associated activity is altered relative to the cell-associated activity of the cell in the absence of the hybrid molecule.
- 10. A method for directing the delivery of the hybrid compound of claim 1 to a particular intracellular location in a cell, the method comprising contacting the cell with the hybrid compound, optionally in the presence of a compound which alters fatty acid composition of Gb3, such that the hybrid compound is delivered to a particular intracellular location in the cell.
- 11. Use of a hybrid compound of claim 1 for the manufacture of a medicament for treatment, prophylaxis, or diagnosis.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority under 35 U.S.C. 119(e) to co-pending U.S. provisional application Serial No. 60/031,668, filed Nov. 22, 1996; No. 60/061,050, filed Oct. 3, 1997; and No. 60/061,044, filed Oct. 4, 1997; the contents of all of which are hereby incorporated by reference.
Provisional Applications (3)
|
Number |
Date |
Country |
|
60031668 |
Nov 1996 |
US |
|
60061050 |
Oct 1997 |
US |
|
60061044 |
Oct 1997 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
08975953 |
Nov 1997 |
US |
Child |
10298408 |
Nov 2002 |
US |