Claims
- 1. A method of forming a coated product comprising the steps of:
- immersing a synthetic organic resin substrate in the form of spheres, particles, sheets, rods or tubes in an aqueous solution containing up to 20 percent by weight of an unsaturated aldehyde containing 4 to 20 carbon atoms;
- irradiating the solution with high energy radiation to form an addition polymerized polyaldehyde layer which is covalently bound to the organic resin surface of the substrate; and
- binding an antibody to the polyaldehyde layer to form a cell specific surface.
- 2. A method according to claim 1 in which the solution further contains from 1 to 50% by weight of a water soluble solvent for the surface of the substrate which is a non-solvent for the coating.
- 3. A method according to claim 2 in which the solvent is selected from dimethyl sulfoxide, tetrahydrofuran and dioxane.
- 4. A method according to claim 1 in which the resin is selected from polyethylene, polypropylene, polystyrene, acrylic polymers or dextran.
- 5. A method according to claim 4 in which the aldehyde is selected from acrolein and C.sub.1 to C.sub.8 aryl, alkyl and cycloalkyl derivatives thereof.
- 6. A method according to claim 5 in which the aldehyde is acrolein.
- 7. A method according to claim 6 further including at least 20% of an additional polymerizable comonomer having a hydrophilic substituent selected from hydroxyl, amino or carboxyl.
- 8. A method according to claim 1 in which the solution contains a suspending agent.
- 9. A method according to claim 8 in which the suspending agent is selected from polyalkylene oxide liquid polymers and an alkali metal alkyl sulfate containing 8 to 20 carbon atoms.
- 10. A method according to claim 9 in which the agent is selected from sodium lauryl sulfate and sodium dodecyl sulfate.
- 11. A method according to claim 1 further including the steps of:
- contacting said surface with a suspension of animal cells; and
- binding those cells containing conjugate antigen receptor sites to said surface.
- 12. A method of forming a coated article comprising the steps of:
- immersing an article having a surface comprising a synthetic organic resin in an aqueous solution containing up to 20 percent by weight of an unsaturated aldehyde containing 4 to 20 carbon atoms and containing a suspending agent, said organic resin being capable of developing covalent bonds during high energy irradiation;
- irradiating the solution with high energy radiation to polymerize said aldehyde in the form of individual microspheres having a uniform diameter; and
- attaching said microspheres to the surface of said article in the form of a continuous layer of contiguous, tangential microspheres by covalent bonds formed between said resin and the microspheres by means of said high energy radiation.
- 13. A method according to claim 12 in which the aldehyde is polymerized to form microspheres having a uniform diameter between 100 Angstroms and 2000 Angstroms.
- 14. A method according to claim 12 in which the article is in the form of spheres, particles, sheets, rods or tubes.
- 15. A method according to claim 14 in which the resin is selected from polyethylene, polypropylene, polystyrene, acrylic polymers or dextran.
- 16. A method according to claim 15 in which the aldehyde is selected from acrolein and C.sub.1 to C.sub.8 aryl, alkyl and cycloalkyl derivatives thereof.
- 17. A method according to claim 16 in which the aldehyde is acrolein.
- 18. A method according to claim 12 in which the suspending agent is selected from polyalkylene oxide liquid polymers and an alkali metal alkyl sulfate containing 8 to 20 carbon atoms.
- 19. A method according to claim 18 in which the agent is selected from sodium lauryl sulfate and sodium dodecyl sulfate.
Parent Case Info
This is a division of application Ser. No. 248,898, filed Mar. 30, 1981 now U.S. Pat. No. 4,438,239.
ORIGIN OF THE INVENTION
The invention described herein was made in the performance of work under a NASA contract and is subject to the provisions of Section 305 of the National Aeronautics and Space Act of 1958, Public Law 83-568 (72 Stat. 435; 42 USC 2457).
US Referenced Citations (7)
Divisions (1)
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Number |
Date |
Country |
Parent |
248898 |
Mar 1981 |
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