Claims
- 1. A method for transfecting a plant with functional heterologous RNA comprising:
- (a) modifying an RNA viral sequence capable of infecting said plant and derived from a first virion to contain said functional heterologous RNA and a nucleic acid sequence comprising an origin of assembly and encoding a coat protein derived from a second virion, said second virion having a morphologically-different type of coat protein from said first virion;
- (b) providing one or more replication gene in cis or in trans to ensure the replication of said modified RNA viral sequence;
- (c) contacting said plant with the elements of (a) and (b) so as to initiate infection; and
- (d) allowing said infection to spread systemically through said plant.
- 2. The method of claim 1 wherein said second virion is a rod-shaped virion.
- 3. The method of claim 1 wherein said second virion is a TMV virion.
- 4. The method of claim 1 wherein said first virion is an icosahedral virion.
- 5. The method of claim 4 wherein said first virion is a BMV virion.
- 6. The method of claim 1 wherein said first virion is a virion of a plant virus.
Parent Case Info
This application is a divisional of Ser. No. 08/445,990, filed May 22, 1995; which is a continuation of Ser. No. 08/158,082, filed Nov. 23, 1993, now abandoned; which is a continuation of Ser. No. 07/978,313, filed Nov. 17, 1992, now abandoned; which is a continuation of Ser. No. 07/518,242, filed May 4, 1990, now abandoned; and which is continuation of Ser. No. 07/012,253, filed Feb. 9, 1987, now abandoned.
Non-Patent Literature Citations (7)
Entry |
Sleat et al. (1986) Virology 155: 299-308. |
Ahlquist et al. (1984) J. Mol. Biol. 172:369-383. |
Meshi et al. (1981) Mol. Gen. Genet. 184:20-25. |
Stanway et al. (1986) J. Virol. 57:1187. |
Semler et al. (1986) Proc. Natl. Acad. Sci. USA 83:1777. |
Takamatsu et al. (1987) EMBO J. 6:307-311. |
French et al. (1986) Science 231:1294-1297. |
Divisions (1)
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Number |
Date |
Country |
Parent |
445990 |
May 1995 |
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Continuations (4)
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Number |
Date |
Country |
Parent |
158082 |
Nov 1993 |
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Parent |
978313 |
Nov 1992 |
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Parent |
518242 |
May 1990 |
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Parent |
12253 |
Feb 1987 |
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