Patent Application
20210185970
This disclosure relates to the field of plant breeding. In particular, this disclosure relates to a new variety of tomato, Solanum lycopersicum variety denominated ‘H1996’.
Breeding improved tomato varieties involves providing genetics that give an advantage to the grower, processor, consumer, or other members of the supply chain. The improvement may be in the form of field performance, disease resistance, factory performance, or a fruit quality characteristic. For a tomato variety to be suitable to be grown for processing, the variety must have a concentrated fruit setting and maturity, firm fruit, and sufficient rot tolerance to allow early fruit to remain rot-free while later fruit continues to develop and ripen.
Most tomato varieties for commercial processing are hybrids resulting from a cross pollination of two true-breeding, inbred parents. Through the use of true-breeding lines, a hybrid is produced that often displays characteristics of each parent, and often demonstrates characteristics that are superior to either parent alone, or that allow a hybrid to mask inadequacies of the individual parents.
Processing tomato varieties combining resistance to verticillium wilt race 1 (Verticillium dahlia), fusarium wilt races 1 and 2 (Fusarium oxysporum pv lycopersici), and root knot nematode (Meloidogyne incognita) are highly desirable in most climates around the world. Moreover, in regions such as California, the industry has seen a considerable increase in pressure from tomato spotted wilt virus (TSWV) and fusarium wilt race 3. Thus, varieties with resistance are in high demand by both growers and processors to ensure a productive crop cycle.
Processing tomato quality parameters differ from those of fruit used in the fresh market. The processing characteristics are typically determined using a sample of hot-break tomato pulp or juice produced in a consistent manner to those familiar with the art. For example, a fixed mass of tomatoes may be cooked in a microwave oven for several minutes to halt any enzymatic breakdown of the sample, lost water is replaced, and the sample is pulped to remove skins and seed to produce a uniform juice sample. The juice sample can be analyzed for various quality parameters important to processing tomato including but not limited to gross viscosity measures such as juice Bostwick, soluble solids measures using a refractometer (°Brix), measures of acidity and pH, and measures of color (e.g., a Hunter a/b score). The value of these traits depends on the product that is being commercially produced by the processing factory. In some instances, a factory will put a higher value on a thick viscosity variety, whereas in other instances, a thin viscosity will make a superior product and is preferred.
Provided herein is a new and distinct tomato variety named ‘H1996’ that produces exceptional yields of medium, uniform fruit and is best adapted to arid climates, such as California, it may also perform reasonably well in Brazil. The broad adaptability of ‘H1996’ is enhanced by a relatively short plant cycle, enabling production in growing regions with shorter seasons. The variety ‘H1996’ has resistance to verticillium wilt race 1, three known races of fusarium wilt, root knot nematode, and tomato spotted wilt virus. The fruit of ‘H1996’ are extremely firm and have very good red color with an average fruit weight of 73 grams. ‘H1996’ presents an advantage over industry standard varieties such as ‘H5608’ in that it has demonstrated resistance to fusarium wilt race 3 which has become a significant production limitation in the state. Tolerance to fruit rot in ‘H1996’ is very good, allowing for once-over machine harvesting applications requiring extended field holding.
In one aspect, the present disclosure is directed to tomato seed designated as ‘H1996’ having ATCC Accession Number PTA-126520. In one aspect, the present disclosure is directed to a tomato plant, as well as any plant part or portion isolated therefrom, produced by growing ‘H1996’ tomato seed. In another aspect, the present disclosure is directed to a tomato plant or part isolated therefrom having all the physiological, morphological, and/or genetic characteristics of a tomato plant produced by growing ‘H1996’ tomato seed having ATCC Accession Number PTA-126520. In yet another aspect, the present disclosure is directed to a tomato plant having all of the physiological and morphological characteristics of tomato variety ‘H1996’, and specifically including those listed in Table 1 below, wherein representative seed is deposited under ATCC Accession Number PTA-126520.
In still another aspect, the present disclosure is directed to tomato seed having at least a first set of the chromosomes of tomato variety ‘H1996’, wherein representative seed is deposited under ATCC Accession Number PTA-126520. In another aspect, the present disclosure is directed to an F1 hybrid tomato seed, methods of making F1 hybrid tomato seed, plants grown from the seed, leaf, ovule, pollen, rootstock, scion, fruit, cotyledon, meristem, anther, root, root tip, pistil, flower, stem, calli, stalk, hypocotyla, pericarp, or portion thereof isolated therefrom having ‘H1996’ as a parent, wherein ‘H1996’ is grown from ‘H1996’ tomato seed having ATCC Accession Number PTA-126520. The disclosure is also directed to a method of producing a tomato plant derived from tomato variety ‘H1996’, including crossing a plant of tomato variety ‘H1996’ with another tomato plant. The method may further comprise harvesting seed from the F1 hybrid tomato seed and/or crossing the F1 hybrid tomato plant with itself or another plant to produce seed from a progeny plant.
Tomato plant parts include leaf, ovule, pollen, rootstock, scion, fruit, cotyledon, meristem, anther, root, root tip, pistil, flower, stem, calli, stalk, hypocotyl, pericarp, the like, and any portion thereof. In another embodiment, the present disclosure is further directed to tomato fruit, stem, leaf, root, root tip, pollen, rootstock, scion, ovule, seed, and flower, and any portion thereof, isolated from ‘H1996’ tomato plants. In one aspect, the plant part comprises at least one cell from tomato variety ‘H1996’. In another aspect, the present disclosure is further directed to tissue culture of regenerable cells derived from ‘H1996’ tomato plants. In one aspect, the tissue culture of regenerable cells has all the physiological and morphological characteristics of tomato variety ‘H1996’. The disclosure is further directed to a tomato plant regenerated from tissue culture. In another aspect, the disclosure is directed to a protoplast produced from tissue culture and a plant regenerated from the protoplast. At least in some approaches, the plant regenerated from the tissue culture or protoplast has all of the physiological and morphological characteristics of tomato variety ‘H1996’, and specifically including those listed in Table 1.
In another aspect, the disclosure is directed to a method for producing a plant part, which at least in one aspect is tomato fruit, and harvesting the plant part. In another aspect, the plant part comprises at least one cell from tomato variety ‘H1996’.
The disclosure also is directed to vegetatively propagating a plant of tomato variety ‘H1996’ by obtaining a part of the plant and regenerating a plant from the plant part. At least in some approaches, the regenerated plant has all of the physiological and morphological characteristics of tomato variety ‘H1996’, and specifically including those listed in Table 1.
In yet another aspect, the present disclosure is further directed to a method of selecting tomato plants by a) growing ‘H1996’ tomato plants wherein the ‘H1996’ plants are grown from tomato seed having ATCC Accession Number PTA-126520; and b) selecting a plant from step a). In another aspect, the present disclosure is further directed to tomato plants, plant parts, and seeds produced by the tomato plants, where the tomato plants are isolated by the selection method.
In another aspect, the present disclosure is further directed to a method of breeding tomato plants by crossing a tomato plant with a plant grown from ‘H1996’ tomato seed having ATCC Accession Number PTA-126520. In another aspect, the tomato plant of tomato variety ‘H1996’ is self-pollinated. In still another aspect, the present disclosure is further directed to tomato plants, tomato parts from the tomato plants, and seeds produced therefrom where the tomato plant is isolated by the breeding method.
In another aspect, the disclosure relates to a plant of tomato variety ‘H1996’ comprising a transgene and/or a single locus conversion, and any seeds or plant parts isolated therefrom. The disclosure also relates to methods for preparing a plant of tomato variety ‘H1996’ comprising a transgene and/or a single locus conversion. In one aspect, the plant comprising a single locus conversion and/or transgene has all the physiological and morphological characteristics of tomato variety ‘H1996’.
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawings will be provided by the office upon request and payment of the necessary fee.
Described herein is the new and distinct tomato variety named ‘H1996’ that was developed to provide a ground-culture hybrid tomato variety (i.e., not grown on stakes) that is suitable for machine harvest, and are adaptable to the climactic conditions of regions such as California, USA.
Processing tomato varieties combining resistance to verticillium wilt race 1 (Verticillium dahlia), fusarium wilt race 1, 2, and 3 (Fusarium oxysporum pv. lycopersici), root knot nematode (Meloidogyne incognita), and tomato spotted wilt virus are highly desirable in many climates around the world. Varieties with tolerance to ripe fruit rots (extended field storage) are also desirable because they are conducive to once-over machine harvesting, particularly in regions where rain can occur during harvest or there are delays in a harvesting schedule.
Tomato plants of ‘H1996’ are resistant to verticillium wilt race 1, three known races of fusarium wilt, root knot nematode, and tomato spotted wilt virus. Large plants are dark green in color with an upright, rather open foliage which exposes the fruit. Fruit of ‘H1996’ are very firm, and medium in size (73 grams). Both internal and external color are strong. Fruit are also considered to have an extended field storage, which delays the onset of fruit rots after reaching red ripe maturity.
The variety is uniform and stable within commercially acceptable limits. As is true with other tomato varieties, a small percentage of variants can occur within commercially acceptable limits for almost any characteristic during the course of repeated multiplication. However, no variants were observed during the three years in which the variety was observed to be uniform and stable.
carotovora)
Fusarium Wilt Race 1
Fusarium Wilt Race 3
Septoria Leaf spot (S.
Lycopersici)
Verticillium Wilt Race 1
Verticillium Wilt Race 2
exigua)
hiritipennis)
Average of 2 years of trials in California in a total of 21 locations.
If more than one category applies, they are listed in rank order.
Data in Table 1 is based primarily upon trials conducted in Collegeville, Calif. from two replications, non-staked, in a research plot environment. Data in Tables 3 and 4 are based upon observations made in trials throughout California and Ontario, Canada. Comparisons among varieties for processing traits (Table 2) were done over two years of side-by side testing throughout California. Disease resistance and adaptability assessments are based upon DNA markers associated with the disease resistance when available or numerous observations collected in regions/climates with specific disease pressure, specifically for ripe fruit rots, bacterial spot, bacterial canker, early blight, and late blight, including Ontario, Canada.
Several characteristics can distinguish ‘H1996’ from the similar commercial variety ‘H5608’. While both varieties have a similar crop maturity, ‘H1996’ is a few days later. ‘H1996’ has a blockier, more rough fruit shape than ‘H5608’ making it less suited for whole peel processing. The plant of ‘H1996’ has a more open foliage cover. From a processing standpoint, ‘H1996’ has a higher serum viscosity than ‘H5608’, which is an advantage to processors looking to produce tomato paste/concentrate for high viscosity products. Agronomically, the major advantage of ‘H1996’ is that it carries resistance to Fusarium oxysporum race 3, which is lacking in ‘H5608’. Another advantage of ‘H1996’ is that its fruit have the ability stay on the vine for an extended period of time and delay the onset of fruit rots (extended field storage), which ‘H5608’ lacks.
Additional methods provided herein include, without limitation, chasing selfs. Chasing selfs involves identifying inbred plants among tomato plants that have been grown from hybrid tomato seed. Once the seed is planted, the inbred plants may be identified and selected due to their decreased vigor relative to the hybrid plants that grow from the hybrid seed. By locating the inbred plants, isolating them from the rest of the plants, and self-pollinating them (i.e., “chasing selfs”), a breeder can obtain an inbred line that is identical to an inbred parent used to produce the hybrid. Accordingly, another aspect of the disclosure relates a method for producing an inbred tomato variety by: planting seed of the tomato variety ‘H1996’; growing plants from the seed; identifying one or more inbred tomato plants; controlling pollination in a manner which preserves homozygosity of the one or more inbred plants; and harvesting resultant seed from the one or more inbred plants. The step of identifying the one or more inbred tomato plants may further include identifying plants with decreased vigor, i.e., plants that appear less robust than plants of the tomato variety ‘H1996’. Tomato plants capable of expressing substantially all of the physiological and morphological characteristics of the parental inbred lines of tomato variety ‘H1996’ include tomato plants obtained by chasing selfs from seed of tomato variety ‘H1996’.
One of ordinary skill in the art will recognize that once a breeder has obtained inbred tomato plants by chasing selfs from seed of tomato variety ‘H1996’, the breeder can then produce new inbred plants such as by sib-pollinating, or by crossing one of the identified inbred tomato plant with a plant of the tomato variety ‘H1996’.
The disclosure further includes introducing one or more desired traits into the tomato variety ‘H1996’. For example, the desired trait may include male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, and drought resistance.
The desired trait may be found on a single gene or combination of genes. The desired trait may be a genetic locus that is a dominant or recessive allele. The genetic locus may be a naturally occurring tomato gene introduced into the genome of a parent of the variety by backcrossing, a natural or induced mutation, or a transgene introduced through genetic transformation techniques. For a genetic locus introduced through transformation, the genetic locus may comprise one or more transgenes integrated at a single chromosomal location. Accordingly, the disclosure provides tomato plants or parts thereof that have been transformed with one or more transgenes (i.e., a genetic locus comprising a sequence introduced into the genome of a tomato plant by transformation) to provide a desired trait. In one aspect, the one or more transgenes are operably linked to at least one regulatory element. In one aspect, the tomato plant or plant part comprising a transgene has all the physiological and morphological characteristics of tomato variety ‘H1996’.
The gene(s) may be introduced to tomato variety ‘H1996’ through a variety of well-known techniques, including for example, molecular biological, other genetic engineering, or plant breeding techniques, such as recurrent selection, backcrossing, pedigree breeding, molecular marker (Isozyme Electrophoresis, Restriction Fragment Length Polymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Amplified Fragment Length Polymorphisms (AFLPs), and Simple Sequence Repeats (SSRs) (also referred to as Microsatellites)), enhanced selection, genetic marker enhanced selection, and transformation. Accordingly, tomato seed, plants, and parts thereof produced by such genetic engineering or plant breed techniques are also part of the present disclosure.
Also provided herein are single locus converted plants and seeds developed by backcrossing wherein essentially all of the morphological and physiological characteristics of an inbred are recovered in addition to the characteristics conferred by the single locus transferred into the inbred via the backcrossing technique. A single locus may comprise one gene, or in the case of transgenic plants, one or more transgenes integrated into the host genome at a single site (locus). One or more locus conversion traits may be introduced into a single tomato variety. In one aspect, the tomato plant or tomato plant part comprising a single locus conversion has all the physiological and morphological characteristics of tomato variety ‘H1996’.
A deposit of the tomato variety ‘H1996’ is maintained by HeinzSeed Company, having an address at 6755 CE Dixon St, Stockton, Calif. 95206, United States of America. Access to this deposit will be available during the pendency of this application to persons determined by the Commissioner of Patents and Trademarks to be entitled thereto under 37 C.F.R. § 1.14 and 35 U.S.C. § 122.
At least 625 seeds of tomato variety ‘H1996’ were deposited on Dec. 2, 2019 according to the Budapest Treaty in the American Type Culture Collection (ATCC), P.O. Box 1549, MANASSAS, Va. 20108 USA. The deposit has been assigned ATCC number PTA-126520. Access to this deposit will be available during the pendency of this application to persons determined by the Commissioner of Patents and Trademarks to be entitled thereto under 37 C.F.R. § 1.14 and 35 U.S.C. § 122. Upon allowance of any claims in this application, all restrictions on the availability to the public of the variety will be irrevocably removed for the enforceable life of the patent.
The deposits will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the effective life of the patent, whichever is longer, and will be replaced if a deposit becomes nonviable during that period.
