Hybridoma Secretion Image Analysis Using Gel Microdrops

Information

• NSF Award
• 9362014

Owner

• One Cell Systems, Inc.

• Award Id

  9362014

• Award Effective Date

  1/15/1994 - 31 years ago

• Award Expiration Date

  3/31/1995 - 29 years ago

• Award Amount

  $ 75,000.00

• Award Instrument

  Standard Grant

9362014 Bradley The goal of this project is to develop a computer based single cell image analysis system based on the company's novel gel microdrop (GMD) technology. This project combines the company's proprietary GMD technology with recent developments in other fields such as fluorescent dye chemistry, microscopy, digital processing, and optics. The core GMD technology allows identification, analysis and separation of single cells based on cell function such as growth, drug susceptibility, protein secretion, enzyme activity, or metabolite production, or cell composition parameters such a surface markers, internal proteins, or nucleic acid sequences. Using a commercially available image analysis system, this project intends to demonstrate feasibility by imaging a model hybridoma secretion assay which will both identify hybridomas secreting higher amounts of antibody and allow recovery for use in biotechnology applications. In Phase II, the company expects to optimize a single cell image analysis system for use in drug discovery and bioprocessing research. ***

• Program Officer

  Kesh S. Narayanan

• Min Amd Letter Date

  1/22/1994 - 31 years ago

• Max Amd Letter Date

  12/30/1994 - 30 years ago

• ARRA Amount

• Name

  ONE CELL SYSTEMS, INC

• City

  CAMBRIDGE

• State

  MA

• Country

  United States

• Address

  100 INMAN ST STE 200

• Postal Code

  021399129

• Phone Number

  6178682399

• First Name

  Kristina

• Last Name

  Lazzari

• Start Date

  4/29/1994 12:00:00 AM

• First Name

  David

• Last Name

  Bradley

• Email Address

  provider@netaxs.com

• Start Date

  1/15/1994 12:00:00 AM

• End Date

  04/29/1994