Claims
- 1. An ECL emission composition comprising (1) an H.sub.2 O.sub.2 -producing or -consuming reactant which produces or consumes H.sub.2 O.sub.2 in the absence of an applied electrical potential, (2) a coreactant which reacts with H.sub.2 O.sub.2, and (3) a metal, colorimetric luminophore that emits light from the reaction between components (2)-(3), in the presence of an oxidizing or reducing electrochemical potential, with the proviso that when (1) contains an H.sub.2 O.sub.2 -consuming reactant, H.sub.2 O.sub.2 is also present.
- 2. A composition capable of emitting electrochemiluminescence comprising:
- (a) at least one species selected from the group consisting of:
- (i) H.sub.2 O.sub.2,
- (ii) an H.sub.2 O.sub.2 producing reactant, and
- (iii) an H.sub.2 O.sub.2 consuming reactant,
- wherein species (iii) is in the presence of H.sub.2 O.sub.2 and species (ii) and (iii) produce or consume H.sub.2 O.sub.2 in the absence of an applied electrical potential,
- (b) a luminophore capable of being induced to electrochemiluminesce;
- (c) an ECL coreactant for said luminophore wherein said ECL coreactant reacts with H.sub.2 O.sub.2.
- 3. A composition according to claim 2, wherein said luminophore is Ru(bpy).sub.3.sup.+2.
- 4. A composition according to claim 2 wherein said H.sub.2 O.sub.2 producing reactant or said H.sub.2 O.sub.2 consuming reactant is at least one substrate that when acted upon by an enzyme produces or consumes H.sub.2 O.sub.2.
- 5. A composition according to claim 2 wherein said reactant, (ii) and/or (iii), is selected from the group consisting of cholesterol, glucose, a triglyceride, glycerol-1-phosphate oxidase, uric acid and ethanol.
- 6. A composition according to claim 2 wherein said coreactant is an oxalate.
- 7. A composition according to claim 2 wherein said H.sub.2 O.sub.2 producing or consuming reactant is at least one enzyme that reacts with a substrate to produce or consume H.sub.2 O.sub.2.
- 8. A composition capable of emitting electrochemiluminescence comprising:
- (a) at least one species selected from the group consisting of:
- (i) H.sub.2 O.sub.2,
- (ii) an H.sub.2 O.sub.2 producing reactant, and
- (iii) an H.sub.2 O.sub.2 consuming reactant,
- wherein species (iii) is in the presence of H.sub.2 O.sub.2 and species (ii) and (iii) produce or consume H.sub.2 O.sub.2 in the absence of an applied electrical potential,
- (b) a luminophore capable of being induced to electrochemiluminesce;
- (c) an ECL coreactant for said luminophore wherein said ECL coreactant reacts with H.sub.2 O.sub.2, and said coreactant is oxalate.
Parent Case Info
This application is a continuation of application Ser. No. 08/482,352, filed Jun. 7, 1995 now allowed, which is incorporated herein by reference.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
5308754 |
Kankare et al. |
May 1994 |
|
5527710 |
Nacamulli et al. |
Jun 1996 |
|
Non-Patent Literature Citations (3)
Entry |
Jameison et al., Anal. Chem., vol. 68, pp. 1298-1302, (1996). |
Kazakov et al., Chemical Abstracts 88:81303, Abstract of Opt. Spek Trosk, vol. 43, No. 6, pp. 1081-1085, (1977). |
Rubenstein et al., J. Am. Chem. Soc., vol. 103, pp. 512-516, (1981). |
Continuations (1)
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Number |
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Parent |
482352 |
Jun 1995 |
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