Claims
- 1. A polynucleotide capture probe for binding a target polynucleotide in a sample to a substrate, wherein said capture probe comprises:
- at least one hydrophobic moiety capable of noncovalently attaching to an unsubstituted substrate selected from the group consisting of glass, nylon, polystyrene, and nitrocellulose; and
- a polynucleotide covalently bound to said at least one hydrophobic moiety,
- wherein said at least one hydrophobic moiety is selected from the group consisting of a naphthalene moiety, a decahydronaphthalene moiety, and a non-polar C.sub.12 to C.sub.50 moiety that is linear, branched, cyclic or aromatic.
- 2. A nucleic acid hybridization assay reagent which comprises the polynucleotide capture probe of claim 1 attached to an unsubstituted substrate selected frown the group consisting of glass, nylon, polystyrene, and nitrocellulose.
- 3. The polynucleotide capture probe of claim 1 wherein the at least one hydrophobic moiety is separated from the polynucleotide by a spacer selected from the group consisting of polypeptide and polymethylene.
- 4. The polynucleotide capture probe of claim 1 wherein the at least one hydrophobic moiety comprises at least one 1-naphthyl group attached to a phosphate moiety on the polynucleotide through an ethylene linkage.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. Ser. No. 08/064,357, filed on May 18, 1993, now abandoned, which is a continuation of U.S. Ser. No. 07/855,448, filed on Mar. 19, 1992, now abandoned, which is a continuation of U.S. Ser. No. 07/374,462, filed on Jun. 30, 1989, now abandoned, All of the referenced applications are incorporated herein by reference in full.
US Referenced Citations (8)
Foreign Referenced Citations (2)
Number |
Date |
Country |
3824110 |
Jan 1990 |
DEX |
WO9010448 |
Sep 1990 |
WOX |
Non-Patent Literature Citations (1)
Entry |
Rubin et al., J. Immunol. Methods (1983) 63:359-366. |
Continuations (3)
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Number |
Date |
Country |
Parent |
64357 |
May 1993 |
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Parent |
855448 |
Mar 1992 |
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Parent |
374462 |
Jun 1989 |
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