Patent Grant
12129257
The present disclosure provides novel imidazo[1,2-a]pyrazines compounds that inhibit protein kinase CK2 activity, compositions containing such compounds, and methods of their preparation. These compounds and compositions are useful as therapeutic agents for treating proliferative disorders such as cancer.
The implication of the protein kinase CK2 in various cellular processes such as cell growth, proliferation, death and differentiation constitute a promising drug target for cancer therapy. The overexpression of protein kinase CK2 was reported in multiple cancer types. Recent evidence suggests that CK2 also acts as a suppressor of apoptosis by phosphorylating pro-apoptotic proteins. To date, only one CK2 inhibitor CX-4945 sold under the name of Silmitasertib, an orally active drug developed by Cylene Pharmaceuticals, is currently in clinical use for the treatment of advanced solid tumors (breast, ovarian, etc.) and multiple myeloma. The astonishing pharmacological profile of the anti-cancer drug, Silmitasertib, opens the door for the development of novel potent and selective CK2 inhibitors for cancer chemotherapy.
Multiple series of CK2 inhibitors have been studied for their activities in inhibiting cell growth alone as well as in combination with other anti-proliferation agents (U.S. Pat. Nos. 9,062,043B2, 7,956,064B2; publication WO 2010080170 A1 and WO 2011011199 A1). Combining CK2 inhibitors with an anti-cancer agent that prohibits cell growth such as alkylating agents, anti-metabolite, vinca alkaloid, taxane, topoisomerase inhibitors, anti-tumor antibiotics, and tyrosine kinase inhibitors to treat or ameliorate a neoplastic disorder has been disclosed in WO 2010080170 A1. Additionally, WO 2010008170 A1 also discloses combining CK2 inhibitors with immunosuppressive macrolide to treat a neoplastic disorder.
Certain CK2 inhibitors are also shown to combine with inhibitors to molecules that are essential in cell growth pathway such as AKT inhibitors, HDAC inhibitors, HSP90 inhibitors, mTOR inhibitors, PBK/niTGR inhibitors, PDK inhibitors, and antibody targeting tumor/cancer antigen to treat or ameliorate neoplastic disorders and/or inflammatory, autoimmune, or infectious disorders (WO 2011011199 A1).
The protein kinase CK2 has been shown to be involved in inflammation, pain, and certain immunological disorders, and other types of diseases such as diabetes, viral infection, neurodegenerative diseases (Borgo et al. Signal Transduction and Targeted Therapy, 183, 2021).
The development of a compound having a novel chemical structure different from that of known CK2 inhibitors and fully satisfiable as a drug for cancer treatment as well as a pharmaceutical composition containing the compound as an active ingredient has been desired. The requirements of antitumor therapeutics call for constant development of new anticancer agents with the aim of generating medicaments that are more potent and well tolerated. Thus, new antitumoral/anti-cancer compounds solving the aforementioned problems are desired.
The present subject matter relates to the use of novel imidazo[1,2-a]pyrazine derivatives with improved potential for treating various types of cancer and with an increased probability of being active against drug resistance cancer cells. The requirements of antitumor therapeutics call for constant development of new anticancer agents with the aim of generating medicaments that are more potent and well tolerated.
The presently described compounds are not only new but have very valuable antitumoral properties. The imidazo[1,2-a]pyrazine compounds, showing inhibitory activity against CK2 enzyme, are potent anticancer agents and may find useful applications in cancer chemotherapy for the fight against various tumors.
The present subject matter provides novel imidazo[1,2-a]pyrazine compounds, for example, imidazo[1,2-a]pyrazine compounds of the general formula/structure I, as useful antitumor agents. The present subject matter further provides a process for the synthesis of such compounds, pharmaceutical compositions containing these compounds, and their use in therapy for the treatment of cancer as a sole active agent or in combination with other active ingredients.
In an embodiment, the present subject matter relates to a compound having the formula I:
or a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof, wherein C:
W may be selected from the group consisting of CH and N;
R may be selected from the group consisting of —Ar, —CH2Ar, —CH2CH2Ar, —CH2CH2NHR1, —CH2CH2NR1R2, —CH2CH2CH2NHR1, and —CH2CH2CH2NR1R2;
Ar may be an aryl ring or a 5 or 6 membered heteroaryl ring, either of the aryl ring or heteroaryl ring being optionally substituted with one or more substituents selected from the group consisting of halogen, cyano, —CF3, —OH, —OR1, —NH2, —NHR1, —NR1R2, —CONH2, —CONHR1, —CONR1R2, —SO2NH2, —SO2NHR1, —SO2NR1R2, —NHCOR1, —NHCO2R1, —NHCONHR1, —NHSO2NHR1, —NHSO2R1, NHCH2COR1, —NHCH2CO2R1, —NHCH2CONHR1, —COR1, —CO2R1, —CH2NHR1, —CH2NR1R2, —OCH2CO2R1, —OCH2CONHR1, —OCH2CONHR1R2, —OCH2COR1, —OCH2CH2R1, —OCH2CONHR1, —OCH2CONR1R2, —OCH2CH2R1, C1-C6 straight chained alkyl, C1-C6 branched alkyl, C3-C6 cycloalkyl, halo-C1-C6 straight chain alkyl, halo-C1-C6 branched alkyl, halo-C3-C6 cycloalkyl, cyano-C1-C6 straight chain alkyl, cyano-C1-C6 branched alkyl, and cyano-C3-C6 cycloalkyl; and
R1 and R2 may each be independently selected from the group consisting of hydrogen, C1-C6 straight chained alkyl, C1-C6 branched alkyl, C3-C6 cycloalkyl, halo-C1-C6 straight chain alkyl, halo-C1-C6 branched alkyl, halo-C3-C6 cycloalkyl, cyano-C1-C6 straight chain alkyl, cyano-C1-C6 branched alkyl, and cyano-C3-C6 cycloalkyl, a halogen atom, a straight or branched C1-C6 alkoxy group, a straight or branched C1-C6 trihaloalkyl group, and a hydroxy group, or wherein R1 and R2, taken together with a nitrogen atom to which they are attached, form a nitrogen-containing heterocycle, such nitrogen-containing heterocycle being optionally substituted with from one to three substituents independently selected from the group consisting of a straight or branched C1-C6 alkyl group, a halogen atom, a straight or branched C1-C6 alkoxy group, a straight or branched C1-C6 trihaloalkyl group, and a hydroxy group.
In another embodiment, the present subject matter relates to a compound having the formula I:
or a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof, wherein:
W may be selected from the group consisting of CH and N;
R may be selected from the group consisting of a phenyl substituted with one or more substituents independently selected from the group consisting of a straight or branched chain C1-C6 alkyl group, halogen, —NR1R2, —CONR1R2, —CF3, and —NHCOCH3; an optionally substituted —CH2phenyl group, an optionally substituted —CH2pyridine group, an optionally substitute-pyridine group, or an optionally substituted —CH2CH2CH2NR1R2 group; and
R1 and R2 are independently a straight chain C1-C6 alkyl group or wherein R1 and R2 are taken together with the nitrogen atom to which they are attached to form a nitrogen-containing heterocycle, such nitrogen-containing heterocycle being optionally substituted with a straight or branched C1-C6 alkyl group.
In an embodiment, the present subject matter relates to a compound selected from the group consisting of: N-(3-fluorophenyl)-2-(pyridin-4-yl)imidazo[1,2-alpyrazin-3-amine (1a); 2-(pyridin-4-yl)-N-(m-tolyl)imidazo[1,2-a]pyrazin-3-amine (2a); 2-(pyridin-4-yl)-N-(3-(trifluoromethyl)phenyl)imidazo[1,2-a]pyrazin-3-amine (3a); N,N-dimethyl-3-((2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)benzamide (4a); N-(3-isopropylphenyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (5a); N1,N1-dimethyl-N3-(2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)benzene-1,3-diamine (6a); N-(3-((2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)phenyl)acetamide (7a); N-(pyridin-2-yl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (8a); N-(3-chlorobenzyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (9a); N-(pyridin-3-ylmethyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (10a); N1,N1-dimethyl-N3-(2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)propane-1,3-diamine (11a); N-(3-(4-methylpiperazin-1-yl)propyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (12a); N-(3-chlorophenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (1b); 2-(pyrimidin-4-yl)-N-(3-(trifluoromethyl)phenyl)imidazo[1,2-a]pyrazin-3-amine (2b); N-(3-isopropylphenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (3b); N1,N1-dimethyl-N3-(2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)benzene-1,3-diamine (4b); N-(3-((2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)phenyl)acetamide (5b); N,N-dimethyl-3-((2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)benzamide (6b); 2-(pyrimidin-4-yl)-N-(m-tolyl)imidazo[1,2-a]pyrazin-3-amine (7b); N-(3-fluorophenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (8b); N-(3-chlorobenzyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (9b); N-(pyridin-3-ylmethyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (10b); N1,N1-dimethyl-N3-(2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)propane-1,3-diamine (11b); N-(3-(4-methylpiperazin-1-yl)propyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (12b); and a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof.
In an embodiment, the present subject matter relates to a process for the synthesis of the compounds of formula I, including a number of species or specific structures falling under structural formula I. Further contemplated herein are pharmaceutical compositions containing these compounds, as well as methods of inhibiting CK2 enzyme activity and of treating various cancers by administering the present compounds to a patient in need thereof.
These and other features of the present subject matter will become readily apparent upon further review of the following specification.
The following definitions are provided for the purpose of understanding the present subject matter and for construing the appended patent claims.
Throughout the application, where compositions are described as having, including, or comprising specific components, or where processes are described as having, including, or comprising specific process steps, it is contemplated that compositions of the present teachings can also consist essentially of, or consist of, the recited components, and that the processes of the present teachings can also consist essentially of, or consist of, the recited process steps.
It is noted that, as used in this specification and the appended claims, the singular forms “a”, “an”, and “the” include plural references unless the context clearly dictates otherwise.
In the application, where an element or component is said to be included in and/or selected from a list of recited elements or components, it should be understood that the element or component can be any one of the recited elements or components, or the element or component can be selected from a group consisting of two or more of the recited elements or components. Further, it should be understood that elements and/or features of a composition or a method described herein can be combined in a variety of ways without departing from the spirit and scope of the present teachings, whether explicit or implicit herein.
The use of the terms “include,” “includes”, “including,” “have,” “has,” or “having” should be generally understood as open-ended and non-limiting unless specifically stated otherwise.
The use of the singular herein includes the plural (and vice versa) unless specifically stated otherwise. In addition, where the use of the term “about” is before a quantitative value, the present teachings also include the specific quantitative value itself, unless specifically stated otherwise. As used herein, the term “about” refers to a ±10% variation from the nominal value unless otherwise indicated or inferred.
As used herein, “halo” or “halogen” refers to fluoro, chloro, bromo, and iodo.
As used herein, “alkyl” refers to a straight-chain or branched saturated hydrocarbon group. Examples of alkyl groups include methyl (Me), ethyl (Et), propyl (e.g., n-propyl and z′-propyl), butyl (e.g., n-butyl, z′-butyl, sec-butyl, tert-butyl), pentyl groups (e.g., n-pentyl, z′-pentyl, -pentyl), hexyl groups, and the like. In various embodiments, an alkyl group can have 1 to 40 carbon atoms (i.e., C1-C40 alkyl group), for example, 1-30 carbon atoms (i.e., C1-C30 alkyl group). In some embodiments, an alkyl group can have 1 to 6 carbon atoms, and can be referred to as a “lower alkyl group” or a “C1-C6 alkyl group”. Examples of lower alkyl groups include methyl, ethyl, propyl (e.g., n-propyl and z′-propyl), and butyl groups (e.g., n-butyl, z′-butyl, sec-butyl, tert-butyl). In some embodiments, alkyl groups can be substituted as described herein. An alkyl group is generally not substituted with another alkyl group, an alkenyl group, or an alkynyl group.
As used herein, “alkenyl” refers to a straight-chain or branched alkyl group having one or more carbon-carbon double bonds. Examples of alkenyl groups include ethenyl, propenyl, butenyl, pentenyl, hexenyl, butadienyl, pentadienyl, hexadienyl groups, and the like. The one or more carbon-carbon double bonds can be internal (such as in 2-butene) or terminal (such as in 1-butene). In various embodiments, an alkenyl group can have 2 to 40 carbon atoms (i.e., C2-C40 alkenyl group), for example, 2 to 20 carbon atoms (i.e., C2-C20 alkenyl group) or 2 to 6 carbon atoms (i.e., C2-C6 alkenyl group). In some embodiments, alkenyl groups can be substituted as described herein. An alkenyl group is generally not substituted with another alkenyl group, an alkyl group, or an alkynyl group.
The term “substituted alkyl” as used herein refers to an alkyl group in which 1 or more (up to about 5, for example about 3) hydrogen atoms is replaced by a substituent independently selected from the group: —O, —S, acyl, acyloxy, optionally substituted alkoxy, optionally substituted amino (wherein the amino group may be a cyclic amine), azido, carboxyl, (optionally substituted alkoxy)carbonyl, amido, cyano, optionally substituted cycloalkyl, optionally substituted cycloalkenyl, halogen, hydroxyl, nitro, sulfamoyl, sulfanyl, sulfinyl, sulfonyl, and sulfonic acid. Some of the optional substituents for alkyl are hydroxy, halogen exemplified by chloro and bromo, acyl exemplified by methylcarbonyl; alkoxy, and heterocyclyl exemplified by morpholino and piperidino. Other alkyl substituents as described herein may further be contemplated.
The term “substituted alkenyl” refers to an alkenyl group in which 1 or more (up to about 5, for example about 3) hydrogen atoms is replaced by a substituent independently selected from those listed above with respect to a substituted alkyl. Other alkenyl substituents as described herein may further be contemplated.
As used herein, “heteroatom” refers to an atom of any element other than carbon or hydrogen and includes, for example, nitrogen, oxygen, silicon, sulfur, phosphorus, and selenium.
As used herein, “aryl” refers to an aromatic monocyclic hydrocarbon ring system or a polycyclic ring system in which two or more aromatic hydrocarbon rings are fused (i.e., having a bond in common with) together or at least one aromatic monocyclic hydrocarbon ring is fused to one or more cycloalkyl and/or cycloheteroalkyl rings. An aryl group can have 6 to 24 carbon atoms in its ring system (e.g., C6-C24 aryl group), which can include multiple fused rings. In some embodiments, a polycyclic aryl group can have 8 to 24 carbon atoms. Any suitable ring position of the aryl group can be covalently linked to the defined chemical structure. Examples of aryl groups having only aromatic carbocyclic ring(s) include phenyl, 1-naphthyl (bicyclic), 2-naphthyl (bicyclic), anthracenyl (tricyclic), phenanthrenyl (tricyclic), pentacenyl (pentacyclic), and like groups. Examples of polycyclic ring systems in which at least one aromatic carbocyclic ring is fused to one or more cycloalkyl and/or cycloheteroalkyl rings include, among others, benzo derivatives of cyclopentane (i.e., an indanyl group, which is a 5,6-bicyclic cycloalkyl/aromatic ring system), cyclohexane (i.e., a tetrahydronaphthyl group, which is a 6,6-bicyclic cycloalkyl/aromatic ring system), imidazoline (i.e., a benzimidazolinyl group, which i a 5,6-bicyclic cycloheteroalkyl/aromatic ring system), and pyran (i.e., a chromenyl group, which is a 6,6-bicyclic cycloheteroalkyl/aromatic ring system). Other examples of aryl groups include benzodioxanyl, benzodioxolyl, chromanyl, indolinyl groups, and the like. In some embodiments, aryl groups can be substituted as described herein. In some embodiments, an aryl group can have one or more halogen substituents, and can be referred to as a “haloaryl” group. Perhaloaryl groups, i.e., aryl groups where all of the hydrogen atoms are replaced with halogen atoms (e.g., —C6F5), are included within the definition of “haloaryl”. In certain embodiments, an aryl group is substituted with another aryl group and can be referred to as a biaryl group. Each of the aryl groups in the biaryl group can be substituted as disclosed herein.
As used herein, “heteroaryl” refers to an aromatic monocyclic ring system containing at least one ring heteroatom selected from oxygen (O), nitrogen (N), sulfur (S), silicon (Si), and selenium (Se) or a polycyclic ring system where at least one of the rings present in the ring system is aromatic and contains at least one ring heteroatom. Polycyclic heteroaryl groups include those having two or more heteroaryl rings fused together, as well as those having at least one monocyclic heteroaryl ring fused to one or more aromatic carbocyclic rings, non-aromatic carbocyclic rings, and/or non-aromatic cycloheteroalkyl rings. A heteroaryl group, as a whole, can have, for example, 5 to 24 ring atoms and contain 1-5 ring heteroatoms (i.e., 5-20 membered heteroaryl group). The heteroaryl group can be attached to the defined chemical structure at any heteroatom or carbon atom that results in a stable structure. Generally, heteroaryl rings do not contain O—O, S—S, or S-0 bonds. However, one or more N or S atoms in a heteroaryl group can be oxidized (e.g., pyridine N-oxide thiophene S-oxide, thiophene S,S-dioxide). Examples of heteroaryl groups include, for example, the 5- or 6-membered monocyclic and 5-6 bicyclic ring systems shown below: where T is O, S, NH, N-alkyl, N-aryl, N-(arylalkyl) (e.g., N-benzyl), SiH2, SiH(alkyl), Si(alkyl)2, SiH(arylalkyl), Si(arylalkyl)2, or Si(alkyl)(arylalkyl). Examples of such heteroaryl rings include pyrrolyl, furyl, thienyl, pyridyl, pyrimidyl, pyridazinyl, pyrazinyl, triazolyl, tetrazolyl, pyrazolyl, imidazolyl, isothiazolyl, thiazolyl, thiadiazolyl, isoxazolyl, oxazolyl, oxadiazolyl, indolyl, isoindolyl, benzofuryl, benzothienyl, quinolyl, 2-methylquinolyl, isoquinolyl, quinoxalyl, quinazolyl, benzotriazolyl, benzimidazolyl, benzothiazolyl, benzisothiazolyl, benzisoxazolyl, benzoxadiazolyl, benzoxazolyl, cinnolinyl, 1H-indazolyl, 2H-indazolyl, indolizinyl, isobenzofuyl, naphthyridinyl, phthalazinyl, pteridinyl, purinyl, oxazolopyridinyl, thiazolopyridinyl, imidazopyridinyl, furopyridinyl, thienopyridinyl, pyridopyrimidinyl, pyridopyrazinyl, pyridopyridazinyl, thienothiazolyl, thienoxazolyl, thienoimidazolyl groups, and the like. Further examples of heteroaryl groups include 4,5,6,7-tetrahydroindolyl, tetrahydroquinolinyl, benzothienopyridinyl, benzofuropyridinyl groups, and the like. In some embodiments, heteroaryl groups can be substituted as described herein.
The term “optional” or “optionally” means that the subsequently described event or circumstance may or may not occur, and that the description includes instances where said event or circumstance occurs and instances in which it does not. For example, “optionally substituted alkyl” means either “alkyl” or “substituted alkyl,” as defined herein.
It will be understood by those skilled in the art with respect to any chemical group containing one or more substituents that such groups are not intended to introduce any substitution or substitution patterns that are sterically impractical and/or physically non-feasible.
The term “isomers” or “stereoisomers” as used herein relates to compounds that have identical molecular formulae but that differ in the arrangement of their atoms in space. Stereoisomers that are not mirror images of one another are termed “diastereoisomers” and stereoisomers that are non-superimposable mirror images are termed “enantiomers,” or sometimes optical isomers. A carbon atom bonded to four non-identical substituents is termed a “chiral center.” Certain compounds herein have one or more chiral centers and therefore may exist as either individual stereoisomers or as a mixture of stereoisomers. Configurations of stereoisomers that owe their existence to hindered rotation about double bonds are differentiated by their prefixes cis and trans (or Z and E), which indicate that the groups are on the same side (cis or Z) or on opposite sides (trans or E) of the double bond in the molecule according to the Cahn-Ingold-Prelog rules. All possible stereoisomers are contemplated herein as individual stereoisomers or as a mixture of stereoisomers.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which the presently described subject matter pertains.
Where a range of values is provided, for example, concentration ranges, percentage ranges, or ratio ranges, it is understood that each intervening value, to the tenth of the unit of the lower limit, unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the described subject matter. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges, and such embodiments are also encompassed within the described subject matter, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the described subject matter.
Throughout the application, descriptions of various embodiments use “comprising” language. However, it will be understood by one of skill in the art, that in some specific instances, an embodiment can alternatively be described using the language “consisting essentially of” or “consisting of”.
“Subject” as used herein refers to any animal classified as a mammal, including humans, domestic and farm animals, and zoo, sports, and pet companion animals such as household pets and other domesticated animals such as, but not limited to, cattle, sheep, ferrets, swine, horses, poultry, rabbits, goats, dogs, cats and the like.
“Patient” as used herein refers to a subject in need of treatment of a condition, disorder, or disease, such as cancer.
For purposes of better understanding the present teachings and in no way limiting the scope of the teachings, unless otherwise indicated, all numbers expressing quantities, percentages or proportions, and other numerical values used in the specification and claims, are to be understood as being modified in all instances by the term “about”. Accordingly, unless indicated to the contrary, the numerical parameters set forth in the following specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained. At the very least, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.
In an embodiment, the present subject matter relates to a compound having the formula I:
or a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof, wherein:
W may be selected from the group consisting of CH and N;
R may be selected from the group consisting of—Ar, —CH2Ar, —CH2CH2Ar, —CH2CH2NHR1, —CH2CH2NR1R2, —CH2CH2CH2NHR1, and—CH2CH2CH2NR1R2;
Ar may be an aryl ring or a 5 or 6 membered heteroaryl ring, either of the aryl ring or heteroaryl ring being optionally substituted with one or more substituents selected from the group consisting of halogen, cyano, —CF3, —OH, —OR1, —NH2, —NHR1, —NR1R2, —CONH2, —CONHR1, —CONR1R2, —SO2NH2, —SO2NHR1, —SO2NR1R2, —NHCOR1, —NHCO2R1, —NHCONHR1, —NHSO2NHR1, —NHSO2R1, NHCH2COR1, —NHCH2CO2R1, —NHCH2CONHR1, —COR1, —CO2R1, —CH2NHR1, —CH2NR1R2, —OCH2CO2R1, —OCH2CONHR1, —OCH2CONHR1R2, —OCH2COR1, —OCH2CH2R1, —OCH2CONHR1, —OCH2CONR1R2, —OCH2CH2R1, C1-C6 straight chained alkyl, C1-C6 branched alkyl, C3-C6 cycloalkyl, halo-C1-C6 straight chain alkyl, halo-C1-C6 branched alkyl, halo-C3-C6 cycloalkyl, cyano-C1-C6 straight chain alkyl, cyano-C1-C6 branched alkyl, and cyano-C3-C6 cycloalkyl; and
R1 and R2 may each be independently selected from the group consisting of hydrogen, C1-C6 straight chained alkyl, C1-C6 branched alkyl, C3-C6 cycloalkyl, halo-C1-C6 straight chain alkyl, halo-C1-C6 branched alkyl, halo-C3-C6 cycloalkyl, cyano-C1-C6 straight chain alkyl, cyano-C1-C6 branched alkyl, and cyano-C3-C6 cycloalkyl, a halogen atom, a straight or branched C1-C6 alkoxy group, a straight or branched C1-C6 trihaloalkyl group, and a hydroxy group, or wherein R1 and R2, taken together with a nitrogen atom to which they are attached, form a nitrogen-containing heterocycle, such nitrogen-containing heterocycle being optionally substituted with from one to three substituents independently selected from the group consisting of a straight or branched C1-C6 alkyl group, a halogen atom, a straight or branched C1-C6 alkoxy group, a straight or branched C1-C6 trihaloalkyl group, and a hydroxy group.
In a further embodiment, the present subject matter relates to compounds of formula I, wherein W may be CH and R may be an optionally substituted —Ar group, an optionally substituted —CH2Ar group, or an optionally substituted —CH2CH2CH2NR1R2 group.
In yet another embodiment, the present subject matter relates to compounds of formula I, wherein W may be CH and R may be a-phenyl substituted with one or more substituents independently selected from the group consisting of halogen, —NR1R2, —CF3, —NHCOR1, one or more straight or branched C1-C6 alkyl, and —CONR1R2.
In still yet another embodiment, the present subject matter relates to compounds of formula I, wherein W may be CH and R may be a-phenyl substituted with one or more substituents independently selected from the group consisting of methyl, fluorine, —N(CH3)2, —CON(CH3)2, —CF3, —NHCOCH3, and isopropyl.
In another embodiment, the present subject matter relates to a compound of formula I, wherein W may be CH and R may be an optionally substituted —CH2phenyl group, —CH2pyridine, an optionally substituted pyridine, or an optionally substituted —CH2CH2CH2NR1R2 group, wherein R1 and R2 are independently a straight chain C1-C6 alkyl group, i.e., wherein R1 and R2 are both methyl, or wherein R1 and R2 are taken together with the nitrogen atom to which they are attached to form a nitrogen-containing heterocycle, such nitrogen-containing heterocycle being optionally substituted with a straight or branched C1-C6 alkyl group, i.e., wherein R1 and R2 are taken together to form a 4-methylpiperazin-1-yl ring. In another embodiment a —CH2phenyl group may be substituted with a chlorine.
In a further embodiment, the present subject matter relates to compounds of formula I, wherein W may be N and R may be an optionally substituted Ar, an optionally substituted CH2Ar, or an optionally substituted —CH2CH2CH2NR1R2.
In yet another embodiment, the present subject matter relates to compounds of formula I, wherein W may be N and R may be a-phenyl substituted with one or more substituents independently selected from the group consisting of halogen, —NR1R2, —CF3, —NHCOR1, one or more straight or branched C1-C6 alkyl, and —CONR1R2.
In still yet another embodiment, the present subject matter relates to compounds of formula I, wherein W may be N and R may be a-phenyl substituted with one or more substituents independently selected from the group consisting of methyl, fluorine, chlorine, —N(CH3)2, —CON(CH3)2, —CF3, —NHCOCH3, and isopropyl.
In another embodiment, the present subject matter relates to a compound of formula I, wherein W may be N and R may be an optionally substituted —CH2phenyl group, —CH2pyridine, or an optionally substituted —CH2CH2CH2NR1R2 group, wherein R1 and R2 are independently a straight chain C1-C6 alkyl group, i.e., wherein R1 and R2 are both methyl, or wherein R1 and R2 are taken together with the nitrogen atom to which they are attached to form a nitrogen-containing heterocycle, such nitrogen-containing heterocycle being optionally substituted with a straight or branched C1-C6 alkyl group, i.e., wherein R1 and R2 are taken together to form a 4-methylpiperazin-1-yl ring. In another embodiment a-CH2phenyl group may be substituted with a chlorine.
In another embodiment, the present subject matter relates to a compound having the formula I:
or a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof, wherein:
W may be selected from the group consisting of CH and N;
R may be selected from the group consisting of a phenyl substituted with one or more substituents independently selected from the group consisting of a straight or branched chain C1-C6 alkyl group, halogen, —NR1R2, —CONR1R2, —CF3, and —NHCOCH3; an optionally substituted —CH2phenyl group, an optionally substituted —CH2pyridine group, an optionally substitute-pyridine group, or an optionally substituted —CH2CH2CH2NR1R2 group; and R1 and R2 are independently a straight chain C1-C6 alkyl group or wherein R1 and R2 are taken together with the nitrogen atom to which they are attached to form a nitrogen-containing heterocycle, such nitrogen-containing heterocycle being optionally substituted with a straight or branched C1-C6 alkyl group.
In an additional embodiment, the present subject matter relates to a compound of Series 1 having the formula:
or a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof, wherein:
R may be selected from the group consisting of a phenyl substituted with one or more substituents independently selected from the group consisting of a straight or branched chain C1-C6 alkyl group, halogen, —NR1R2, —CONR1R2, —CF3, and —NHCOCH3; an optionally substituted —CH2phenyl group, an optionally substituted —CH2pyridine group, an optionally substitute-pyridine group, or an optionally substituted —CH2CH2CH2NR1R2 group; and
In an embodiment, the present subject matter relates to a compound falling with this genus selected from the group consisting of: N-(3-fluorophenyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (1a); 2-(pyridin-4-yl)-N-(m-tolyl)imidazo[1,2-alpyrazin-3-amine (2a); 2-(pyridin-4-yl)-N-(3-(trifluoromethyl)phenyl)imidazo[1,2-aJpyrazin-3-amine (3a); N,N-dimethyl-3-((2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)benzamide (4a); N-(3-isopropylphenyl)-2-(pyridin-4-yl)imidazo[1,2-aJpyrazin-3-amine (5a); N1,N1-dimethyl-N3-(2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)benzene-1,3-diamine (6a); N-(3-((2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)phenyl)acetamide (7a); N-(pyridin-2-yl)-2-(pyridin-4-yl)imidazo[1,2-alpyrazin-3-amine (8a); N-(3-chlorobenzyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (9a); N-(pyridin-3-ylmethyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (10a); N1,N1-dimethyl-N3-(2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)propane-1,3-diamine (11a); N-(3-(4-methylpiperazin-1-yl)propyl)-2-(pyridin-4-yl)imidazo[1,2-alpyrazin-3-amine (12a); and a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof.
Said differently, the present subject matter can relate to compounds selected from the group consisting of
and a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof.
In yet another embodiment, the present subject matter relates to a compound of Series 2 having the formula:
or a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof, wherein:
R may be selected from the group consisting of a phenyl substituted with one or more substituents independently selected from the group consisting of a straight or branched chain C1-C6 alkyl group, halogen, —NR1R2, —CONR1R2, —CF3, and —NHCOCH3; an optionally substituted-CH2phenyl group, an optionally substituted —CH2pyridine group, an optionally substitute-pyridine group, or an optionally substituted —CH2CH2CH2NR1R2 group; and
R1 and R2 are independently a straight chain C1-C6 alkyl group or wherein R1 and R2 are taken together with the nitrogen atom to which they are attached to form a nitrogen-containing heterocycle, such nitrogen-containing heterocycle being optionally substituted with a straight or branched C1-C6 alkyl group.
In an embodiment, the present subject matter relates to a compound falling with this genus selected from the group consisting of: N-(3-chlorophenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (1b); 2-(pyrimidin-4-yl)-N-(3-(trifluoromethyl)phenyl)imidazo[1,2-a]pyrazin-3-amine (2b); N-(3-isopropylphenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (3b); N1,N1-dimethyl-N3-(2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)benzene-1,3-diamine (4b); N-(3-((2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)phenyl)acetamide (5b); N,N-dimethyl-3-((2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)benzamide (6b); 2-(pyrimidin-4-yl)-N-(m-tolyl)imidazo[1,2-a]pyrazin-3-amine (7b); N-(3-fluorophenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (8b); N-(3-chlorobenzyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (9b); N-(pyridin-3-ylmethyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (10b); N1,N1-dimethyl-N3-(2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)propane-1,3-diamine (11b); N-(3-(4-methylpiperazin-1-yl)propyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (12b); and a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof.
Said differently, the present subject matter can relate to compounds selected from the group consisting of:
and a pharmaceutically acceptable salt, ester, stereoisomer, or solvate thereof.
It is to be understood that the present subject matter covers all combinations of substituent groups referred to herein.
The present compounds may contain, e.g., when isolated in crystalline form, varying amounts of solvents. Accordingly, the present subject matter includes all solvates of the present compounds of formula I and pharmaceutically acceptable stereoisomers, esters, and/or salts thereof. Hydrates are one example of such solvates.
Further, the present subject matter includes all mixtures of possible stereoisomers of the embodied compounds, independent of the ratio, including the racemates.
Salts of the present compounds, or salts of the stereoisomers thereof, include all inorganic and organic acid addition salts and salts with bases, especially all pharmaceutically acceptable inorganic and organic acid addition salts and salts with bases, particularly all pharmaceutically acceptable inorganic and organic acid addition salts and salts with bases customarily used in pharmacy.
Examples of acid addition salts include, but are not limited to, hydrochlorides, hydrobromides, phosphates, nitrates, sulfates, acetates, trifluoroacetates, citrates, D-gluconates, benzoates, 2-(4-hydroxy-benzoyl)benzoates, butyrates, subsalicylates, maleates, laurates, malates, lactates, fumarates, succinates, oxalates, tartrates, stearates, benzenesulfonates (besilates), toluenesulfonates (tosilates), methanesulfonates (mesilates) and 3-hydroxy-2-naphthoates.
Examples of salts with bases include, but are not limited to, lithium, sodium, potassium, calcium, aluminum, magnesium, titanium, ammonium, meglumine and guanidinium salts. The salts include water-insoluble and, particularly, water-soluble salts.
The present compounds, the salts, the stereoisomers and the salts of the stereoisomers thereof may contain, e.g., when isolated in crystalline form, varying amounts of solvents. Included within the present scope are, therefore, all solvates of the compounds of formula I, as well as the solvates of the salts, the stereoisomers and the salts of the stereoisomers of the compounds of formula I.
The present compounds may be isolated and purified in a manner known per se, e.g., by distilling off the solvent in vacuo and recrystallizing the residue obtained from a suitable solvent or subjecting it to one of the customary purification methods, such as column chromatography on a suitable support material.
Salts of the compounds of formula I and the stereoisomers thereof can be obtained by dissolving the free compound in a suitable solvent (by way of non-limiting example, a ketone such as acetone, methylethylketone or methylisobutylketone; an ether such as diethyl ether, tetrahydrofurane or dioxane; a chlorinated hydrocarbon such as methylene chloride or chloroform; a low molecular weight aliphatic alcohol such as methanol, ethanol or isopropanol; a low molecular weight aliphatic ester such as ethyl acetate or isopropyl acetate; or water) which contains the desired acid or base, or to which the desired acid or base is then added. The acid or base can be employed in salt preparation, depending on whether a mono- or polybasic acid or base is concerned and depending on which salt is desired, in an equimolar quantitative ratio or one differing therefrom. The salts are obtained by filtering, reprecipitating, precipitating with a non-solvent for the salt or by evaporating the solvent. Salts obtained can be converted into the free compounds which, in turn, can be converted into salts. In this manner, pharmaceutically unacceptable salts, which can be obtained, for example, as process products in the manufacturing on an industrial scale, can be converted into pharmaceutically acceptable salts by processes known to the person skilled in the art.
Pure diastereomers and pure enantiomers of the present compounds can be obtained, e.g., by asymmetric synthesis, by using chiral starting compounds in synthesis and by splitting up enantiomeric and diastereomeric mixtures obtained in synthesis. Preferably, the pure diastereomeric and pure enantiomeric compounds are obtained by using chiral starting compounds in synthesis.
Enantiomeric and diastereomeric mixtures can be split up into the pure enantiomers and pure diastereomers by methods known to a person skilled in the art. Preferably, diastereomeric mixtures are separated by crystallization, in particular fractional crystallization, or chromatography. Enantiomeric mixtures can be separated, e.g., by forming diastereomers with a chiral auxiliary agent, resolving the diastereomers obtained and removing the chiral auxiliary agent. As chiral auxiliary agents, for example, chiral acids can be used to separate enantiomeric bases and chiral bases can be used to separate enantiomeric acids via formation of diastereomeric salts. Furthermore, diastereomeric derivatives such as diastereomeric esters can be formed from enantiomeric mixtures of alcohols or enantiomeric mixtures of acids, respectively, using chiral acids or chiral alcohols, respectively, as chiral auxiliary agents. Additionally, diastereomeric complexes or diastereomeric clathrates may be used for separating enantiomeric mixtures. Alternatively, enantiomeric mixtures can be split up using chiral separating columns in chromatography. Another suitable method for the isolation of enantiomers is enzymatic separation.
In one embodiment, the present compounds of Series 1 can be prepared according to the following general synthetic pathway. Specifically, synthesis commences with adding, to a solution of pyrazin-2-amine II in CH2Cl2, pyridine-4-carboxaldehyde I and perchloric acid. After stirring at room temperature for about 1 h, the ter-butylisocyanide III is added. The reaction mixture is stirred at room temperature for about 24 h and concentrated under reduced pressure. The resulting residue is purified to provide N-(tert-butyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV as outlined in Scheme 1.
Next, to a solution of N-(tert-butyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV in CH2Cl2 is added TFA and the resulting mixture is stirred at room temperature for about 24 h. The resulting mixture is evaporated, and the residue is dissolved in water. The pH is adjusted to about 9 by adding a solution of 20% Na2CO3. The resulting precipitate is filtrated, washed with water, and recrystallized to furnish 2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine V as outlined in Scheme 2.
For compound (1a)-(8a), a mixture of 2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine V, arylhalide, NaOtBu, and XPhos Pd G2 in t-BuOH is obtained. The resulting mixture is flushed with argon and heated at about 75° C. for about 5 h. After completion of the reaction, brine solution is added and extracted with CH2Cl2. The combined organic layers are dried over magnesium sulfate and concentrated under reduced pressure. The resulting residue is purified to yield 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI (compounds 1a-8a) as outlined in Scheme 3.
For compounds (9a) and (10a), a mixture of 2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine V and an aldehyde derivative is stirred at room temperature. After stirring for about 2 h, sodium cyanoborohydride is added by small portion to the reaction mixture and stirred at room temperature for an additional about 2 h. The solvent is then concentrated under vacuum and the resulting crude product is diluted in CH2Cl2 and washed with 10% HCl. The organic layer is dried over anhydrous magnesium sulfate, filtrated, and concentrated to afford the residue which is purified to give the corresponding 3-N-benzylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VII as outlined in Scheme 4.
For compounds (11a) and (12a), to a solution of 2-(pyridin-4-yl)imidazo[1,2-alpyrazin-3-amine V in DMF are added triethyl amine, potassium iodide, and alkylchloride and the reaction mixture is stirred at room temperature for about 18 hours. The reaction solvent is concentrated under vacuum and the resulting crude product is treated with 10% NaOH and extracted with CH2Cl2. The combined organic layers are dried over anhydrous magnesium sulfate, filtrated, and concentrated to afford the residue which is purified to yield 3-alkylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VIII as outlined in Scheme 5.
In an alternative embodiment, the present compounds of Series 2 can be prepared according to the following general synthetic pathway. Specifically, synthesis commences with adding, to a solution of pyrazin-2-amine II in CH2Cl2 pyrimidine-4-carboxaldehyde I and perchloric acid. After stirring at room temperature for about 1 h, the ter-butylisocyanide III is added. The reaction mixture is stirred at room temperature for about 24 h and concentrated under reduced pressure. The resulting residue is purified to provide N-(tert-butyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV as outlined in alternative Scheme 1.
Next, to a solution of N-(tert-butyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV in CH2Cl2 is added TFA and the resulting mixture is stirred at room temperature for about 24 h. The resulting mixture is evaporated, and the residue is dissolved in water. The pH is adjusted to about 9 by adding a solution of 20% Na2CO3. The resulting precipitate is filtrated, washed with water, and recrystallized to furnish 2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine V as outlined in alternative Scheme 2.
For compound (1b)-(8b), a mixture of 2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine V, arylhalide, NaOtBu, and XPhos Pd G2 in t-BuOH is obtained. The resulting mixture is flushed with argon and heated at about 75° C. for about 5 h. After completion of the reaction, brine solution is added and extracted with CH2Cl2. The combined organic layers are dried over magnesium sulfate and concentrated under reduced pressure. The resulting residue is purified to yield 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI (compounds 1a-8a) as outlined in alternative Scheme 3.
For compounds (9b) and (10b), a mixture of 2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine V and an aldehyde derivative is stirred at room temperature. After stirring for about 2 h, sodium cyanoborohydride is added by small portion to the reaction mixture and stirred at room temperature for an additional about 2 h. The solvent is then concentrated under vacuum and the resulting crude product is diluted in CH2Cl2 and washed with 10% HCl. The organic layer is dried over anhydrous magnesium sulfate, filtrated, and concentrated to afford the residue which is purified to give the corresponding 3-N-benzylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VII as outlined in alternative Scheme 4.
For compounds (11b) and (12b), to a solution of 2-(pyrimidin-4-yl)imidazo[1,2-alpyrazin-3-amine V in DMF are added triethyl amine, potassium iodide, and an alkylchloride and the reaction mixture is stirred at room temperature for about 18 hours. The reaction solvent is concentrated under vacuum and the resulting crude product is treated with 10% NaOH and extracted with CH2Cl2. The combined organic layers are dried over anhydrous magnesium sulfate, filtrated, and concentrated to afford the residue which is purified to yield 3-alkylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VIII as outlined in alternative Scheme 5.
In another embodiment, the present subject matter is directed to pharmaceutical compositions comprising a therapeutically effective amount of the compounds as described herein together with one or more pharmaceutically acceptable carriers, excipients, or vehicles. In some embodiments, the present compositions can be used for combination therapy, where other therapeutic and/or prophylactic ingredients can be included therein.
The present subject matter further relates to a pharmaceutical composition, which comprises at least one of the present compounds together with at least one pharmaceutically acceptable auxiliary.
In an embodiment, the pharmaceutical composition comprises one or two of the present compounds, or one of the present compounds.
Non-limiting examples of suitable excipients, carriers, or vehicles useful herein include liquids such as water, saline, glycerol, polyethylene glycol, hyaluronic acid, ethanol, and the like. Suitable excipients for nonliquid formulations are also known to those of skill in the art. A thorough discussion of pharmaceutically acceptable excipients and salts useful herein is available in Remington's Pharmaceutical Sciences, 18th Edition. Easton, Pa., Mack Publishing Company, 1990, the entire contents of which are incorporated by reference herein.
The present compounds are typically administered at a therapeutically or pharmaceutically effective dosage, e.g., a dosage sufficient to provide treatment for cancer. Administration of the compounds or pharmaceutical compositions thereof can be by any method that delivers the compounds systemically and/or locally. These methods include oral routes, parenteral routes, intraduodenal routes, and the like.
While human dosage levels have yet to be optimized for the present compounds, generally, a daily dose is from about 0.01 to 10.0 mg/kg of body weight, for example about 0.1 to 5.0 mg/kg of body weight. The precise effective amount will vary from subject to subject and will depend upon the species, age, the subject's size and health, the nature and extent of the condition being treated, recommendations of the treating physician, and the therapeutics or combination of therapeutics selected for administration. The subject may be administered as many doses as is required to reduce and/or alleviate the signs, symptoms, or causes of the disease or disorder in question, or bring about any other desired alteration of a biological system.
In employing the present compounds for treatment of cancer, any pharmaceutically acceptable mode of administration can be used with other pharmaceutically acceptable excipients, including solid, semi-solid, liquid or aerosol dosage forms, such as, for example, tablets, capsules, powders, liquids, suspensions, suppositories, aerosols or the like. The present compounds can also be administered in sustained or controlled release dosage forms, including depot injections, osmotic pumps, pills, transdermal (including electrotransport) patches, and the like, for the prolonged administration of the compound at a predetermined rate, preferably in unit dosage forms suitable for single administration of precise dosages.
The present compounds may also be administered as compositions prepared as foods for humans or animals, including medical foods, functional food, special nutrition foods and dietary supplements. A “medical food” is a product prescribed by a physician that is intended for the specific dietary management of a disorder or health condition for which distinctive nutritional requirements exist and may include formulations fed through a feeding tube (referred to as enteral administration or gavage administration).
A “dietary supplement” shall mean a product that is intended to supplement the human diet and may be provided in the form of a pill, capsule, tablet, or like formulation. By way of non-limiting example, a dietary supplement may include one or more of the following dietary ingredients: vitamins, minerals, herbs, botanicals, amino acids, and dietary substances intended to supplement the diet by increasing total dietary intake, or a concentrate, metabolite, constituent, extract, or combinations of these ingredients, not intended as a conventional food or as the sole item of a meal or diet. Dietary supplements may also be incorporated into foodstuffs, such as functional foods designed to promote control of glucose levels. A “functional food” is an ordinary food that has one or more components or ingredients incorporated into it to give a specific medical or physiological benefit, other than a purely nutritional effect. “Special nutrition food” means ingredients designed for a particular diet related to conditions or to support treatment of nutritional deficiencies.
Generally, depending on the intended mode of administration, the pharmaceutically acceptable composition will contain about 0.1% to 90%, for example about 0.5% to 50%, by weight of a compound or salt of the present compounds, the remainder being suitable pharmaceutical excipients, carriers, etc.
One manner of administration for the conditions detailed above is oral, using a convenient daily dosage regimen which can be adjusted according to the degree of affliction. For such oral administration, a pharmaceutically acceptable, non-toxic composition is formed by the incorporation of any of the normally employed excipients, such as, for example, mannitol, lactose, starch, magnesium stearate, sodium saccharine, talcum, cellulose, sodium croscarmellose, glucose, gelatin, sucrose, magnesium carbonate, and the like. Such compositions take the form of solutions, suspensions, tablets, dispersible tablets, pills, capsules, powders, sustained release formulations and the like.
The present compositions may take the form of a pill or tablet and thus the composition may contain, along with the active ingredient, a diluent such as lactose, sucrose, dicalcium phosphate, or the like; a lubricant such as magnesium stearate or the like; and a binder such as starch, gum acacia, polyvinyl pyrrolidine, gelatin, cellulose, and derivatives thereof, and the like.
Liquid pharmaceutically administrable compositions can, for example, be prepared by dissolving, dispersing, etc. an active compound as defined above and optional pharmaceutical adjuvants in a carrier, such as, for example, water, saline, aqueous dextrose, glycerol, glycols, ethanol, and the like, to thereby form a solution or suspension. If desired, the pharmaceutical composition to be administered may also contain minor amounts of nontoxic auxiliary substances such as wetting agents, emulsifying agents, or solubilizing agents, pH buffering agents and the like, for example, sodium acetate, sodium citrate, cyclodextrin derivatives, sorbitan monolaurate, triethanolamine acetate, triethanolamine oleate, etc.
For oral administration, a pharmaceutically acceptable non-toxic composition may be formed by the incorporation of any normally employed excipients, such as, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, talcum, cellulose derivatives, sodium croscarmellose, glucose, sucrose, magnesium carbonate, sodium saccharin, talcum and the like. Such compositions take the form of solutions, suspensions, tablets, capsules, powders, sustained release formulations and the like.
For a solid dosage form, a solution or suspension in, for example, propylene carbonate, vegetable oils or triglycerides, may be encapsulated in a gelatin capsule. Such diester solutions, and the preparation and encapsulation thereof, are disclosed in U.S. Pat. Nos. 4,328,245; 4,409,239; and 4,410,545, the contents of each of which are incorporated herein by reference. For a liquid dosage form, the solution, e.g., in a polyethylene glycol, may be diluted with a sufficient quantity of a pharmaceutically acceptable liquid carrier, e.g., water, to be easily measured for administration.
Alternatively, liquid or semi-solid oral formulations may be prepared by dissolving or dispersing the active compound or salt in vegetable oils, glycols, triglycerides, propylene glycol esters (e.g., propylene carbonate) and the like, and encapsulating these solutions or suspensions in hard or soft gelatin capsule shells.
Other useful formulations include those set forth in U.S. Pat. Nos. Re. 28,819 and 4,358,603, the contents of each of which are hereby incorporated by reference.
Another manner of administration is parenteral administration, generally characterized by injection, either subcutaneously, intramuscularly, or intravenously. Injectables can be prepared in conventional forms, either as liquid solutions or suspensions, solid forms suitable for solution or suspension in liquid prior to injection, or as emulsions. Suitable excipients are, for example, water, saline, dextrose, glycerol, ethanol or the like. In addition, if desired, the pharmaceutical compositions to be administered may also contain minor amounts of non-toxic auxiliary substances such as wetting or emulsifying agents, pH buffering agents, solubility enhancers, and the like, such as for example, sodium acetate, sorbitan monolaurate, triethanolamine oleate, cyclodextrins, etc.
Another approach for parenteral administration employs the implantation of a slow-release or sustained-release system, such that a constant level of dosage is maintained. The percentage of active compound contained in such parenteral compositions is highly dependent on the specific nature thereof, as well as the activity of the compound and the needs of the subject. However, percentages of active ingredient of 0.01% to 10% in solution are employable and will be higher if the composition is a solid which will be subsequently diluted to the above percentages. The composition may comprise 0.2% to 2% of the active agent in solution.
Nasal solutions of the active compound alone or in combination with other pharmaceutically acceptable excipients can also be administered.
Formulations of the active compound or a salt may also be administered to the respiratory tract as an aerosol or solution for a nebulizer, or as a microfine powder for insufflation, alone or in combination with an inert carrier such as lactose. In such a case, the particles of the formulation have diameters of less than 50 microns, for example less than 10 microns.
The present compounds have valuable pharmaceutical properties, which make them commercially utilizable. Accordingly, the present subject matter further relates to use of the present compounds for the treatment of diseases such as cancers. Similarly, the present compounds can be used to inhibit CK2 enzyme activity in a patient.
In another embodiment of the present subject matter, the aforementioned compounds demonstrated in vitro anticancer action against human cancer cell lines such as MCF7 and MDA-MB-231 (breast cancer), H1299 (lung cancer), PC3 (prostate cancer), HCT116 (colon cancer), A375 (melanoma), MIAPaCa-2 (pancreatic cancer), and HL60 (leukemia). Accordingly, the present subject matter relates to methods of treating a cancer in a patient by administering one or more of the compounds presented herein to a patient in need thereof. In certain embodiments, the cancer treatable with the present compounds is one or more selected from the group consisting of leukemia, melanoma, colon cancer, prostate cancer, lung cancer, pancreatic cancer, and breast cancer.
Accordingly, in an embodiment of the present subject matter, the imidazo[1,2-alpyrazine compounds as described, i.e., the 3-substituted amino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine compounds of Series 1 and the 3-substituted amino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine compounds of Series 2, herein engaged for in vitro study towards human cancer cell lines can display an IC50 with a nano to micromolar concentration range when exposed to a period of at least 96 hours.
For example, a present compound of Series 1 engaged for in vitro study against PC3 (prostate) cancer cell lines can display an IC50 concentration of 1.2 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 1 engaged for in vitro study against HCT116 (colon) cancer cell lines for IC50 of 1.7 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 1 engaged for in vitro study against A375 (melanoma) cancer cell lines for IC50 of 3.4 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 1 engaged for in vitro study against H1299 (lung) cancer cell lines for IC50 of 1.5 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 1 engaged for in vitro study against MIAPaCa-2 (pancreas) cancer cell lines for IC50 of 1.3 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 1 engaged for in vitro study against HL60 (leukemia) cancer cell lines for IC50 of 4.4 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 1 engaged for in vitro study against MCF7 (breast) cancer cell lines for IC50 of 5.1 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 1 engaged for in vitro study against MDA-MB-231 (breast) cancer cell lines for IC50 of 4.6 μM at an exposure period of at least 96 hours.
In another embodiment, a present compound of Series 2 engaged for in vitro study against PC3 (prostate) cancer cell lines can display an IC50 concentration of 1.4 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 2 engaged for in vitro study against HCT116 (colon) cancer cell lines for IC50 of 1.4 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 2 engaged for in vitro study against A375 (melanoma) cancer cell lines for IC50 of 3.5 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 2 engaged for in vitro study against H1299 (lung) cancer cell lines for IC50 of 1.2 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 2 engaged for in vitro study against MIAPaCa-2 (pancreas) cancer cell lines for IC50 of 1.6 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 2 engaged for in vitro study against HL60 (leukemia) cancer cell lines for IC50 of 4.5 M at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 2 engaged for in vitro study against MCF7 (breast) cancer cell lines for IC50 of 5.1 μM at an exposure period of at least 96 hours.
In another embodiment of the presently claimed subject matter, the concentration of the compounds of Series 2 engaged for in vitro study against MDA-MB-231 (breast) cancer cell lines for IC50 of 5.0 μM at an exposure period of at least 96 hours.
The present subject matter further relates to a method of treating or preventing a disease comprising administering to a patient in need thereof a therapeutically effective amount of at least one of the compounds herein.
In particular, the present subject matter relates to a method of treating one of the above-mentioned diseases or disorders comprising administering to a patient in need thereof a therapeutically effective amount of at least one of the compounds herein.
In the above methods, the patient is preferably a mammal, more preferably a human. Furthermore, in the above methods, at least one of the present compounds can be used. In an embodiment, one or two of the present compounds are used, or one of the present compounds is used. Similarly, one or more of the present compounds can be used in combination therapy with one or more additional active agents.
The following examples relate to various methods of manufacturing certain specific compounds as described herein. All compound numbers expressed herein are with reference to the synthetic pathway figures shown above.
Preparation of N-(tert-butyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV
To a solution of pyrazin-2-amine II (2 mmol) in CH2Cl2 (2.5 mL) were added pyridine-4-carboxaldehyde I (2.2 mmol) and perchloric acid (0.1 mmol). After stirring at room temperature for 1 h, the ter-butylisocyanide III (2.2 mmol) was added. The reaction mixture was stirred at room temperature for 24 h and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography to provide N-(tert-butyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV.
Elemental Analysis: Calculated C, 67.39; H, 6.41; N, 26.20; Found C, 67.33; H, 6.45; N, 26.14.
Preparation of 2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine V
To a solution of N-(tert-butyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV (1 mmol) in CH2Cl2 (5 mL) was added TFA (5 mL) and the resulting mixture was stirred at room temperature for 24 h. The resulting mixture was evaporated, and the residue was dissolved in water (5 mL). The pH was adjusted to 9 by adding a solution of 20% Na2CO3. The resulting precipitate was filtrated, washed with water and recrystallized to furnish 2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine V.
Elemental Analysis: Calculated C, 62.55; H, 4.29; N, 33.16; Found C, 62.58; H, 4.26; N, 33.21.
General procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI (compounds 1a-8a)
A mixture of 2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine V (0.5 mmol), arylhalide (0.6 mmol), NaOtBu (3 mmol), and XPhos Pd G2 (0.25 mmol) in t-BuOH (2 mL) was obtained. The resulting mixture was flushed with argon and heated at 75° C. for 5 h. After completion of the reaction, brine solution (20 mL) was added and extracted with CH2Cl2 (4×20 mL). The combined organic layers were dried over magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography to yield 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI (compounds 1a-8a).
Preparation of N-(3-fluorophenyl)-2-(pyridin-4-yl)imidazo| 1,2-a]pyrazin-3-amine (1a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 3, using 1-fluoro-3-iodobenzene as the arylhalide.
Elemental Analysis: Calculated C, 66.88; H, 3.96; N, 22.94; Found C, 66.82; H, 3.99; N, 23.00.
Preparation of 2-(pyridin-4-yl)-N-(m-tolyl)imidazo[1,2-a]pyrazin-3-amine (2a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 3, using 3-bromotoluene as the arylhalide.
Elemental Analysis: Calculated C, 71.74; H, 5.02; N, 23.24; Found C, 71.71; H, 4.96; N, 23.21.
Preparation of 2-(pyridin-4-yl)-N-(3-(trifluoromethyl)phenyl)imidazo[1,2-a]pyrazin-3-amine (3a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 3, using 1-bromo-3-trifluorobenzene as the arylhalide.
Elemental Analysis: Calculated C, 60.85; H, 3.40; N, 19.71; Found C, 60.80; H, 3.36; N, 19.75.
Preparation of N,N-dimethyl-3-((2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)benzamide (4a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 3, using 3-iodo-N,N-dimethylbenzamide as the arylhalide.
Elemental Analysis: Calculated C, 67.02; H, 5.06; N, 23.45; Found C, 66.97; H, 5.03; N, 23.49.
Preparation of N-(3-isopropylphenyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (5a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 3, using 1-bromo-3-isopropylbenzene as the arylhalide.
Elemental Analysis: Calculated C, 72.92; H, 5.81; N, 21.26; Found C, 72.88; H, 5.86; N, 21.29.
Preparation of N1, N1-dimethyl-N3-(2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)benzene-1,3-diamine (6a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 3, using N,N-dimethyl-3-bromoaniline as the arylhalide.
Elemental Analysis: Calculated C, 69.07; H, 5.49; N, 25.44; Found C, 69.02; H, 5.44; N, 25.41.
Preparation of N-(3-((2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)phenyl)acetamide (7a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 3, using N-(3-iodophenyl)acetamide as the arylhalide.
Elemental Analysis: Calculated C, 66.27; H, 4.68; N, 24.40; Found C, 66.23; H, 4.72; N, 24.46.
Preparation of N-(pyridin-2-yl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (8a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 3, using 2-bromopyridine as the arylhalide.
Elemental Analysis: Calculated C, 66.66; H, 4.20; N, 29.15; Found C, 66.71; H, 4.17; N, 29.19.
General procedure for the preparation of 3-N-benzylamino-2-(pyridin-4-yl)imidazo[1,2-alpyrazine VII (compounds 9a and 10a)
A mixture of 2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine V (1 mmol) and an aldehyde derivative (1.2 mmol) was stirred at room temperature. After stirring for 2 h, sodium cyanoborohydride (2 mmol) was added by small portion to the reaction mixture and stirred at room temperature for an additional 2 h. The solvent was then concentrated under vacuum and the resulting crude product was diluted in CH2Cl2 (15 mL) and washed with 10% HCl (3×10 mL). The organic layer was dried over anhydrous magnesium sulfate, filtrated, and concentrated to afford the residue which was purified by silica gel column chromatography to give the corresponding 3-N-benzylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VII (compounds 9a and 10a).
Preparation of N-(3-chlorobenzyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (9a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-N-benzylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VII, of Example 12, using 3-chlorobenzaldehyde as the aldehyde derivative.
Elemental Analysis: Calculated C, 64.38; H, 4.20; N, 20.86; Found C, 64.33; H, 4.25; N, 20.81.
Preparation of N-(pyridin-3-ylmethyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (10a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-N-benzylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VII, of Example 12, using pyridine-3-carboxaldehyde as the aldehyde derivative.
Elemental Analysis: Calculated C, 67.54; H, 4.67; N, 27.80; Found C, 67.49; H, 4.71; N, 27.86.
General procedure for the preparation of 3-alkylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VIII (compounds 11a and 12a)
To a solution of 2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine V (1 mmol) in DMF (10 mL) were added triethyl amine (1.5 mmol), potassium iodide (1.2 mmol), and an alkylchloride (1.2 mmol) and the reaction mixture was stirred at room temperature for 18 hours. The reaction solvent was concentrated under vacuum and the resulting crude product was treated with 10% NaOH (10 mL) and extracted with CH2Cl2 (3×5 mL). The combined organic layers were dried over anhydrous magnesium sulfate, filtrated and concentrated to afford the residue which was purified by silica gel column chromatography to yield 3-alkylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VIII.
Preparation of N1,N1-dimethyl-N3-(2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)propane-1,3-diamine (11a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-alkylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VIII, of Example 15, using 2-chloro-N,N-dimethylpropan-1-amine as the alkylchloride.
Elemental Analysis: Calculated C, 64.84; H, 6.80; N, 28.36; Found C, 64.80; H, 6.73; N, 28.41.
Preparation of N-(3-(4-methylpiperazin-1-yl)propyl)-2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-amine (12a)
The expected product is obtained in accordance with the general procedure for the preparation of 3-alkylamino-2-(pyridin-4-yl)imidazo[1,2-a]pyrazine VIII, of Example 15, using 1-(3-chloropropyl)-4-methylpiperazine as the alkylchloride.
Elemental Analysis: Calculated C, 64.93; H, 7.17; N, 27.90; Found C, 64.88; H, 7.21; N, 27.86.
Preparation of N-(tert-butyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV
To a solution of pyrazin-2-amine II (2 mmol) in CH2Cl2 (2.5 mL) were added pyrimidine-4-carboxaldehyde I (2.2 mmol) and perchloric acid (0.1 mmol). After stirring at room temperature for 1 h, the ter-butylisocyanide III (2.2 mmol) was added. The reaction mixture was stirred at room temperature for 24 h and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography to provide N-(tert-butyl)-2-(pyridimin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV.
Elemental Analysis: Calculated C, 62.67; H, 6.01; N, 31.32; Found C, 62.62; H, 5.97; N, 31.35.
Preparation of 2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine V
To a solution of N-(tert-butyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine IV (1 mmol) in CH2Cl2 (5 mL) was added TFA (5 mL) and the resulting mixture was stirred at room temperature for 24 h. The resulting mixture was evaporated, and the residue was dissolved in water (5 mL). The pH was adjusted to 9 by adding a solution of 20% Na2CO3. The resulting precipitate was filtrated, washed with water and recrystallized to furnish 2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine V.
Elemental Analysis: Calculated C, 56.60; H, 3.80; N, 39.60; Found C, 56.55; H, 3.76; N, 39.66.
General procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-alpyrazine VI (compounds 1b-8b)
A mixture of 2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine V (0.5 mmol), arylhalide (0.6 mmol), NaOtBu (3 mmol), and XPhos Pd G2 (0.25 mmol) in t-BuOH (2 mL) was obtained. The resulting mixture was flushed with argon and heated at 75° C. for 5 h. After completion of the reaction, brine solution (20 mL) was added and extracted with CH2Cl2 (4×20 mL). The combined organic layers were dried over magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography to yield 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI (compounds 1b-8b).
Preparation of N-(3-chlorophenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (1b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 20, using 1-chloro-3-iodobenzene as the arylhalide.
Elemental Analysis: Calculated C, 59.54; H, 3.44; N, 26.04; Found C, 59.49; H, 3.41; N, 26.00.
Preparation of 2-(pyrimidin-4-yl)-N-(3-(trifluoromethyl)phenyl)imidazo[1,2-a]pyrazin-3-amine (2b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 20, using 1-bromo-3-trifluorobenzene as the arylhalide.
Elemental Analysis: Calculated C, 57.31; H, 3.11; N, 23.59; Found C, 57.27; H, 3.08; N, 23.63.
Preparation of N-(3-isopropylphenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (3b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 20, using 1-bromo-3-isopropylbenzene as the arylhalide.
Elemental Analysis: Calculated C, 69.07; H, 5.49; N, 25.44; Found C, 69.02; H,
5.51; N, 25.49.
Preparation of N1,N1-dimethyl-N3-(2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)benzene-1,3-diamine (4b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 20, using N,N-dimethyl-3-bromoaniline as the arylhalide.
Elemental Analysis: Calculated C, 65.24; H, 5.17; N, 29.59; Found C, 65.22; H, 5.14; N, 29.63.
Preparation of N-(3-((2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)phenyl)acetamide (5b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 20, using N-(3-iodophenyl)acetamide as the arylhalide.
Elemental Analysis: Calculated C, 62.60; H, 4.38; N, 28.39; Found C, 62.58; H, 4.34; N, 28.37.
Preparation of N,N-dimethyl-3-((2-(pyridin-4-yl)imidazo[1,2-a]pyrazin-3-yl)amino)benzamide (6b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 20, using 3-iodo-N,N-dimethylbenzamide as the arylhalide.
Elemental Analysis: Calculated C, 63.50; H, 4.77; N, 27.28; Found C, 63.46; H, 4.81; N, 27.33.
Preparation of 2-(pyrimidin-4-yl)-N-(m-tolyl)imidazo[1,2-a]pyrazin-3-amine (7b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 20, using 3-bromotoluene as the arylhalide.
Elemental Analysis: Calculated C, 67.54; H, 4.67; N, 27.80; Found C, 67.49; H, 4.71; N, 27.78.
Preparation of N-(3-fluorophenyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (8b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-arylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VI, or Example 20, using 1-bromo-3-fluorobenzene as the arylhalide.
Elemental Analysis: Calculated C, 62.74; H, 3.62; N, 27.44; Found C, 62.69; H, 3.58; N, 27.41.
General procedure for the preparation of 3-N-benzylamino-2-(pyrimidin-4-yl)imidazo[1,2-alpyrazine VII (compounds 9b and 10b)
A mixture of 2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine V (1 mmol) and an aldehyde derivative (1.2 mmol) was stirred at room temperature. After stirring for 2 h, sodium cyanoborohydride (2 mmol) was added by small portion to the reaction mixture and stirred at room temperature for an additional 2 h. The solvent was then concentrated under vacuum and the resulting crude product was diluted in CH2Cl2 (15 mL) and washed with 10% HCl (3×10 mL). The organic layer was dried over anhydrous magnesium sulfate, filtrated, and concentrated to afford the residue which was purified by silica gel column chromatography to give the corresponding 3-N-benzylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VII (compounds 9b and 10b).
Preparation of N-(3-chlorobenzyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (9b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-N-benzylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VII, of Example 29, using 3-chlorobenzaldehyde as the aldehyde derivative.
Elemental Analysis: Calculated C, 60.63; H, 3.89; N, 24.95; Found C, 60.66; H, 3.85; N, 24.99.
Preparation of N-(pyridin-3-ylmethyl)-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine (10b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-N-benzylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VII, of Example 29, using pyridine-3-carboxaldehyde as the aldehyde derivative.
Elemental Analysis: Calculated C, 63.36; H, 4.32; N, 32.32; Found C, 63.32; H, 4.26; N, 32.37.
General procedure for the preparation of 3-alkylamino-2-(pyrimidin-4-yl)imidazo[1,2-alpyrazine VIII (compounds 11b and 12b)
To a solution of 2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-amine V (1 mmol) in DMF (10 mL) were added triethyl amine (1.5 mmol), potassium iodide (1.2 mmol), and an alkylchloride (1.2 mmol) and the reaction mixture was stirred at room temperature for 18 hours. The reaction solvent was concentrated under vacuum and the resulting crude product was treated with 10% NaOH (10 mL) and extracted with CH2Cl2 (3×5 mL). The combined organic layers were dried over anhydrous magnesium sulfate, filtrated and concentrated to afford the residue which was purified by silica gel column chromatography to yield 3-alkylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VIII.
Preparation of N1,N1-dimethyl-N3-(2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazin-3-yl)propane-1,3-diamine (11b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-alkylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VIII, of Example 32, using 2-chloro-N,N-dimethylpropan-1-amine as the alkylchloride.
Elemental Analysis: Calculated C, 60.59; H, 6.44; N, 32.97; Found C, 60.64; H, 6.39; N, 33.02.
Preparation of N-(3-(4-methylpiperazin-1-yl)propyl)-2-(pyrimidin-4-yl)imidazo[1,2-alpyrazin-3-amine (12b)
The expected product is obtained in accordance with the general procedure for the preparation of 3-alkylamino-2-(pyrimidin-4-yl)imidazo[1,2-a]pyrazine VIII, of Example 32, using 1-(3-chloropropyl)-4-methylpiperazine as the alkylchloride.
Elemental Analysis: Calculated C, 61.34; H, 6.86; N, 31.79; Found C, 61.29; H, 6.81; N, 31.84.
Pharmacological Activity
In vitro Cytotoxic Activity Assay
Compounds 1a-12a and 1b-12b were screened for their in vitro cytotoxic activity utilizing a 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against selected cancer human cell lines consisting of PC3 (prostate), HCT116 (colon), A375 (melanoma), H1299 (lung), MIAPaCa-2 (pancreas), HL60 (leukemia), MCF7 (breast), MDA-MB-231 (breast) (T. Mosmann, J. Immunol. Meth., 1983, 65, 55-63). The cells were cultured at 37° C. in RMPI1640 medium supplemented with 10% fetal bovine serum, 50 IU/mL penicillin, and 50 μg/mL streptomycin in a 5% CO2 incubator. All cells were sub-cultured 3 times/week by trypsinisation. Viable cells were seeded and allowed to adhere for 12 h before a test drug was added in 96-well plates at an initial density of 1.0×105 cells/mL. Tumor cell lines were separately exposed to various concentrations of the tested compounds followed by incubation at a temperature of 37° C. during 96 hours inside a medium of fresh RMPI1640. Cells were subsequently incubated at 37° C. using MTT at 0.5 mg/mL during 4 hours. After removal of supernatant, formazan crystals were dissolved in isopropanol and the optical density was measured at 570 nm. CX-4945 was used as a positive control.
By way of example, the compound (3a) displayed promising anti-proliferative activity against human cancer cells as reported in Table 1.
aCells were exposed for 96 hours and the number of viable cells was measured using the MTS reagent. IC50 values were calculated as the concentration of compound eliciting a 50% inhibition of cell proliferation expressed in μM.
The biological results demonstrated that the compound (3a) displayed promising in vitro anti-proliferative activity against various human cancer cell lines similar to that of CX-4945 used as reference drug.
Similarly, the compound (2b) displayed promising anti-proliferative activity against human cancer cells as reported in Table 1.
aCells were exposed for 96 hours and the number of viable cells was measured using the MTS reagent. IC50 values were calculated as the concentration of compound eliciting a 50% inhibition of cell proliferation expressed in μM.
The biological results demonstrated that the compound (2b) displayed promising in vitro anti-proliferative activity against various human cancer cell lines similar to that of CX-4945 used as reference drug.
Evaluation of Inhibitory Activity on protein kinase CK2
CK2 Kinase Assay was conducted using the protocol as described in Pierre F. et al; J. Med. Chem. 2011, 54, 635-654. The tested compounds in aqueous solution were added at a volume of 10 μL to a reaction mixture comprising 10 μL of assay dilution buffer (ADB; 20 mM MOPS, pH 7.2, 25 mM β-glycerol phosphate, 5 mM EGTA, 1 mM sodium orthovanadate, and 1 mM dithiothreitol), 10 μL of substrate peptide (RRRDDDSDDD, dissolved in ADB at a concentration of 1 mM), 10 μL of recombinant human CK2 (RRBB-holoenzyme, 25 ng dissolved in ADB; Millipore). Reactions were initiated by the addition of 10 μL of ATP solution (90%75 mMMgCl2, 75 μM ATP (final ATP concentration: 15 μM) dissolved in ADB; 10% [γ-33P] ATP (stock 1mCi/100 μL; 3000 Ci/mmol (Perkin-Elmer) and maintained for 10 min at 30° C. The reactions were quenched with 100 μL of 0.75% phosphoric acid and then transferred to and filtered through a phosphor cellulose filter plate (Millipore). After washing each well five times with 0.75% phosphoric acid, the plate was dried under vacuum for 5 min and, following the addition of 15 μL of scintillation fluid to each well, the residual radioactivity was measured using a Wallac luminescence counter. The IC50 values were derived from eight concentrations of test inhibitors.
The biological results demonstrated that the present compounds possessed favourable CK2 inhibition with IC50 at a nanomolar concentration range.
By way of example, the compound (3a) displayed promising protein kinase CK2 activity with an IC50 of 115 nM, while in the same experimental condition, the reference control CX-4945 inhibited protein kinase CK2 activity of 1 nM.
Similarly, the compound (2b) displayed promising protein kinase CK2 activity with an IC50 of 98 nM, while in the same experimental condition, the reference control CX-4945 inhibited protein kinase CK2 activity of 1 nM.
It is to be understood that the imidazo[1,2-a]pyrazine compounds are not limited to the specific embodiments described above, but encompasses any and all embodiments within the scope of the generic language of the following claims enabled by the embodiments described herein, or otherwise shown in the drawings or described above in terms sufficient to enable one of ordinary skill in the art to make and use the claimed subject matter.
| Number | Name | Date | Kind |
|---|---|---|---|
| 20050136065 | Valiante, Jr. | Jun 2005 | A1 |
| 20070149535 | Berset et al. | Jun 2007 | A1 |
| 20160280685 | Baizaitov et al. | Sep 2016 | A1 |
| Number | Date | Country |
|---|---|---|
| 115286629 | Nov 2022 | CN |
| 2010083617 | Jul 2010 | WO |
| 2022164934 | Aug 2022 | WO |
| Entry |
|---|
| FDA approved solvents, Q3C—Tables and List, Guidance for Industry, U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Center for Biologics Evaluation and Research (CBER), ICH, Revision 3, Jun. 2017 (Year: 2017). |
| Donella-Deana et al., Tyrosine phosphorylation of protein kinase CK2 by Src-related tyrosine kinases correlates with increased catalytic activity, Biochemical Journal, vol. 372, 841-849, Mar. 11, 2003 (Year: 2003). |
| Alsfouk AA, Alshibl HM, Alsfouk BA, Altwaijry NA, Al-Abdullah ES. “Synthesis and Biological Evaluation of Imadazo [1,2-a]pyrazines as Anticancer and Antiviral Agents through Inhibition of CDK9 and Human Coronavirus”, Pharmaceuticals (Basel). Jul. 13, 2022;15(7):859. |
| Dichiara, Maria, et al. “Structure-Property Optimization of a Series of Imidazopyridines for Visceral Leishmaniasis”, ACS Infectious Diseases 9.8 (2023): 1470-1487. |
| Dong, Ru, et al. “Design, synthesis and anticancer evaluation of 3-methyl-1H-indazole derivatives as novel selective bromodomain-containing protein 4 inhibitors.” Bioorganic & Medicinal Chemistry 55 (2022): 116592. |
