Patent Grant
7566734
The present invention is directed to compounds and compositions containing said compounds which bind selectively to somatostatin receptor subtypes and the use of said compounds for treating medical disorders which are mediated by somatostatin receptor subtypes. Somatostatin (somatotropin release inhibiting factor, SRIF), a tetradecapeptide hormone, originally isolated from bovine hypothalami (Brazesu, P. et al., Science 179, 77-79, 1973) has been shown to have a wide range of regulatory effects on the release of a variety of hormones such as growth hormone, prolactin, glucagon, insulin, gastrin (Bloom, S. R. and Poldack, J. M., Brit. Med. J. 295, 288-289, 1987). In addition, antiproliferative properties (Reichlin, S., N. Engl. J. Med. 309, 1495-1501, 1983) have been obtained with somatostatin analogs in metastatic prostatic cancer (Parmar, H. et al, Clin. Exp. Metastasis, 10, 3-11, 1992) and in several other neuroendocrine neoplasms in man (Anthony, L. et al. Acta Oncol., 32, 217-223, 1993). Metabolism of somatostatin by aminopeptidases and carboxypeptidases leads to a short duration of action.
The actions of somatostatin are mediated via membrane bound receptors. The heterogeneity of its biological functions has led to studies to identify structure-activity relationships of peptides analogs at the somatostatin receptors which resulted in the discovery of five receptor subtypes (Yamada, et al, Proc. Natl. Acad. Sci. U.S.A 89, 251-255, 1992; Raynor, K. et al. Mol. Pharmacol., 44, 385-392, 1993). The functional roles of these receptors are under extensive investigation. Binding to the different types of somatostatin subtypes have been associated with the treatment of the following conditions and/or diseases. Activation of types 2 and 5 have been associated with growth hormone suppression and more particularly GH secreting adenomas (Acromegaly) and TSH secreting adenomas. Activation of type 2 but not type 5 has been associated with treating prolactin secreting adenomas. Other indications associated with activation of the somatostatin subtypes are restenosis, inhibition of insulin and/or glucagon and more particularly diabetes mellitus, hyperlipidemia, insulin insensitivity. Syndrome X, angiopathy, proliferative retinopathy, dawn phenomenon and Nephropathy; inhibition of gastric acid secretion and more particularly peptic ulcers, entercutaneous and pancreaticocutaneous fistula, irritable bowel syndrome, Dumping syndrome, watery diarrhea syndrome, AIDS related diarrhea, chemotherapy-induced diarrhea, acute or chronic pancreatitis and gastrointestinal hormone secreting tumors; treatment of cancer such as hepatoma; inhibition of angiogenesis, treatment of inflammatory disorders such as arthritis; chronic allograft rejection; angioplasty; preventing graft vessel and gastrointestinal bleeding. Somatostatin agonists can also be used for decreasing body weight in a patent.
In drug research, it is a key issue to minimize side effects by developing highly potent and selective drug molecules. Recent work on the development of nonpeptide structures (Hirshmann, R. et al, J. Am. Chem. Soc. 115, 12550-12568, 1993; Papageorgiou, C. and Borer, X., Bioorg. Med. Chem. Lett. 6, 267-272, 1996) have described compounds with low somatostatin receptor affinity.
The present invention is directed to a family of nonpeptide compounds, which are selective and potent somatostatin receptor ligands.
In one aspect the present invention is directed to a compound of the formula (I),
the racemic-diastereomeric mixtures and optical isomers of said compound of formula (I), the pharmaceutically-acceptable salts and prodrugs thereof or a pharmaceutically acceptable salt thereof,
wherein
A preferred compound of formula I is where R1 is H; R2 is H; R3 is —CH2-phenyl; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl; R6 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —CH2-phenyl; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl; R4 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —CH2-phenyl; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl; R4 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —CH2-indol-3-yl; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl or t-Bu; R6 is H;
Another compound of formula (I) is where R1 is H; R2 is H; R3 is —CH2-indol-3-yl; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl or t-Bu; R6 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —CH2-indol-3-yl; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl or t-Bu; R6 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —CH2-indol-3-yl; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl or t-Bu; R6 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —CH2-indol-3-yl, —(CH2)4—NH—CO—O-t-Bu or —(CH2)4—NH2; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl, o-methoxyphenyl, p-Br-phenyl, p-nitro-phenyl or p-N,N-diethylamino-phenyl; R4 is H;
Of the immediately above compounds it is preferred that when R5 is phenyl and R3 is —(CH2)-indol-3-yl that the stereochemistry at the carbon to which R3 is attached is in the R-configuration.
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —CH2-indol-3-yl, —(CH2)4—NH—CO—O-t-Bu or —(CH2)4—NH2; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl, o-methoxyphenyl, p-methoxyphenyl, p-Br-phenyl, p-nitro-phenyl or p-N,N-diethylamino-phenyl; R1 is H;
Of the immediately foregoing compounds when R5 is phenyl or o-OMe-phenyl and R3 is —(CH2)-indol-3-yl; it is preferred that the compounds are the separated enantiomers (R or S configuration) according to the carbon to which R3 is attached.
Another preferred compound of formula (I) is where R1 is H; R3 is —(CH2)4—NH—CO—O-t-Bu or —(CH2)4—NH2; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl; R6 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —(CH2)4—NH—CO—O-t-Bu or —(CH2)4—NH2; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl; R6 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —(CH2)m-indol-3-yl, —(CH2)4—NH—CO—O-t-Bu or —(CH2)4—NH2; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl, o-OMe-phenyl or p-OMe-phenyl; R4 is H;
Another preferred compound of formula (I) is where R1 is H; R2 is H; R3 is —(CH2)4—NH—CO—O-t-Bu or —(CH2)4—NH2; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl; R6 is H;
Another preferred compound of formula (I) is where R1 is —(CH2)—CO-Z1; R2 is H; R3 is —(CH2)4—NH—CO—O-t-Bu, —(CH2)4—NH—CO—O-benzyl, —(CH2)-phenyl or —(CH2)-indol-3-yl; R4 is —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl; R6 is H;
Another preferred compound of formula (I) is where R1 is —(CH2)—CO—(CH2)m-Z1 where m in the definition of R1 is 0, 1 or 2; R2 is H; R3 is —(CH2)-indol-3-yl or —(CH2)4—NH—CO—O-t-Bu; R4 is H or —(CH2)m-A1 where m in the definition of R4 is 0; R5 is phenyl, o-OMe-phenyl, p-nitro-phenyl, p-Br-phenyl, t-Bu, —CH(CH3)2—CO—NH—(CH2)2—CO—O-t-Bu, —CH(CH3)2—CO—NH—(CH2)3-imidazol-1-yl, —CH(CH3)2—CO—NH—(CH2)2-pyridin-2-yl, —CH(CH3)2—CO—NH—(CH2)3-4-morpholino, —CH(CH3)2—CO—NH—(CH2)-pyridin-4-yl or —CH(CH3)2—CO—NH—(CH2)2—N,N-diethylamino; R3 is H;
Another preferred compound of formula (I) is where R1 and R2 are taken together to form a compound of formula (Ib) or (Ic);
In another respect, the present invention is directed to a compound of the formula (II),
the racemic-diastereomeric mixtures and optical isomers of said compound of formula (II), the pharmaceutically-acceptable salts or prodrugs thereof or a pharmaceutically acceptable salt of said prodrug,
wherein
A preferred group of compounds of formula (II), have the following formula:
Another preferred group of compounds of formula (II) have the following formula:
and
Yet another preferred group of compounds of formula (II) have the following formula:
wherein X2 is p-chloro-phenyl, p-methoxy-phenyl, 2,4-difluoro-phenyl or thienyl.
Still another preferred group of compounds of formula (II) have the following formula:
wherein X2 is p-chloro phenyl, p-methoxy-phenyl, phenyl or thienyl.
Further still a preferred compound of formula (II) has the following formula:
Further still another preferred compound of formula (II) has the following formula:
Further still another preferred group of compounds of formula (II) have the following formula:
In another aspect, this invention is directed to a pharmaceutical composition comprising one or more of a compound of formula (I) or formula (II), as defined hereinabove, and a pharmaceutically acceptable carrier.
In another aspect, the present invention is directed to a method of eliciting an agonist effect from one or more of a somatostatin subtype receptor in a subject in need thereof, which comprises administering a compound of formula (I) or formula (II) or a pharmaceutically acceptable salt thereof to said subject.
In another aspect, the present invention is directed to a method of eliciting an antagonist effect from one or more of a somatostatin subtype receptor in a subject in need thereof, which comprises administering a compound of formula (I) or formula (II) or a pharmaceutically acceptable salt thereof to said subject.
In another aspect, the present invention is directed to a method of binding one or more of a somatostatin subtype receptor in a subject in need thereof, which comprises administering a compound of formula (I) or formula (II) or a pharmaceutically acceptable salt thereof to said subject.
In another aspect, the present invention is directed to a method of treating acromegaly, restenosis, Crohn's disease, systemic sclerosis, external and internal pancreatic pseudocysts and ascites, VIPoma, nesidoblastosis, hyperinsulinism, gastrinoma, Zollinger-Ellison syndrome, diarrhea, AIDS related diarrhea, chemotherapy related diarrhea, scleroderma, Irritable Bowel Syndrome, pancreatitis, small bowel obstruction, gastroesophageal reflux, duodenogastric reflux, Cushing's Syndrome, gonadotropinoma, hyperoparathyroidism, Graves' Disease, diabetic neuropathy, Paget's disease, polycystic ovary disease, cancer, cancer cachexia, hypotension, postprandial hypotension, panic attacks, GH secreting adenomas or TSH secreting adenomas, in a subject in need thereof, which comprises administering a compound of formula (I) or formula (II) or a pharmaceutically acceptable salt thereof to said subject.
In another aspect, the present invention is directed to a method of treating diabetes mellitus, hyperlipidemia, insulin insensitivity, Syndrome X, angiopathy, proliferative retinopathy, dawn phenomenon, Nephropathy, peptic ulcers, enterocutaneous and pancreaticocutaneous fistula, Dumping syndrome, watery diarrhea syndrome, acute or chronic pancreatitis, gastrointestinal hormone secreting tumors, angiogenesis, inflammatory disorders, chronic allograft rejection, angioplasty, graft vessel bleeding or gastrointestinal bleeding in a subject in need thereof, which comprises administering a compound of formula (I) or formula (II) or a pharmaceutically acceptable salt thereof to said subject.
In another aspect, the present invention is directed to a method of inhibiting the proliferation of helicobacter pylori in a subject in need thereof, which comprises administering a compound of formula (I) or formula (II) or a pharmaceutically acceptable salt thereof, to said subject.
One of ordinary skill will recognize that certain substituents listed in the invention may have reduced chemical stability when combined with one another or with heteroatoms in the compounds. Such compounds with reduced chemical stability are not preferred.
In general, the compounds of Formula I or II can be made by processes which include processes known in the chemical arts for the production of compounds. Certain processes for the manufacture of Formula I or II compounds are provided as further features of the invention and are illustrated by the following reaction schemes and examples.
In the above structural formulae and throughout the instant application, the following terms have the indicated meanings unless expressly stated otherwise.
The alkyl groups are intended to include those alkyl groups of the designated length in either a straight or branched configuration. Exemplary of such alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, tertiary butyl, pentyl, isopentyl, hexyl, isohexyl, and the like.
When the definition “C0-alkyl” occurs in the definition, it means a single covalent bond.
The alkoxy groups specified above are intended to include those alkoxy groups of the designated length in either a straight or branched configuration. Exemplary of such alkoxy groups are methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, tertiary butoxy, pentoxy, isopentoxy, hexoxy, isohexoxy and the like.
The term halogen or halo is intended to include the halogen atoms fluorine, chlorine, bromine and iodine.
The term cycloalkyl is intended to include a mono-cycloalkyl group or a bi-cycloalkyl group of the indicated carbon number known to those of skill in the art.
The term aryl is intended to include aromatic rings known in the art, which can be mono-cyclic, bi-cyclic or tri-cyclic, such as phenyl, naphthyl and anthracene.
The term heterocycle includes mono-cyclic, bi-cyclic and tri-cyclic systems having one or more heteroatoms, such as oxygen, nitrogen and/or sulfur. The ring systems may be aromatic, for example pyridine, indole, quinoline, pyrimidine, thiophene (also known as thienyl), furan, benzothiophene, tetrazole, dihydroindole, indazolyl, N-formylindole, benzimidazole, thiazole, and thiadiazole. The ring systems may be non-aromatic, for example pyrrolidine, piperidine, morpholine and the like.
The chemist of ordinary skill will recognize that certain combinations of heteroatom-containing substituents listed in this invention define compounds which will be less stable under physiological conditions. Accordingly, such compounds are less preferred.
When a chemical structure as used herein has an arrow emanating from it, the arrow indicates the point of attachment. For example, the structure
is a pentyl group. When an arrow is drawn through a cyclic moiety, the arrow indicates that the cyclic moiety can be attached at any of the available bonding points, for example
means that the phenyl can be bonded ortho, meta or para to the X group. When an arrow is drawn through a bi-cyclic or a tri-cyclic moiety, the arrow indicates that the bi-cyclic or tri-cyclic ring can be attached at any of the available bonding points in any of the rings, for example
means that the indole is bonded either through the phenyl portion of the ring or the nitrogen containing ring portion.
The compounds of the instant invention have at least one asymmetric center as noted by the asterisk in the structural formula (I), (Ia) and (Ib), above. Additional asymmetric centers may be present on the molecule depending upon the nature of the various substituents on the molecule. Each such asymmetric center will produce two optical isomers and it is intended that all such optical isomers, as separated, pure or partially purified optical isomers, racemic mixtures or diastereomeric mixtures thereof, be included within the scope of the instant invention.
The instant compounds can be generally isolated in the form of their pharmaceutically acceptable acid addition salts, such as the salts derived from using inorganic and organic acids. Examples of such acids are hydrochloric, nitric, sulfuric, phosphoric, acetic, propionic, maleic, succinic, D-tartaric, L-tartaric, malonic, methane sulfonic and the like. In addition, certain compounds containing an acidic function such as a carboxy can be isolated in the form of their inorganic salt in which the counter-ion can be selected from sodium, potassium, lithium, calcium, magnesium and the like, as well as from organic bases.
The pharmaceutically acceptable salts are formed by taking about 1 equivalent of a compound of formula (I) or (II) and contacting with about 1 equivalent of the appropriate corresponding acid of the salt which is desired. Work-up and isolation of the resulting salt is well-known to those of ordinary skill in the art.
As is known in the art, agonists and antagonists of somatostatin are useful for treating a variety of medical conditions and diseases, such as inhibition of H. pylori proliferation, acromegaly, restenosis, Crohn's disease, systemic sclerosis, external and internal pancreatic pseudocysts and ascites, VIPoma, nesidoblastosis, hyperinsulinism, gastrinoma, Zollinger-Ellison Syndrome, diarrhea, AIDS related diarrhea, chemotherapy related diarrhea, scleroderma, Irritable Bowel Syndrome, pancreatitis, small bowel obstruction, gastroesophageal reflux, duodenogastric reflux and in treating endocrinological diseases and/or conditions, such as Cushing's Syndrome, gonadotropinoma, hyperparathyroidism, Graves' Disease, diabetic neuropathy, Paget's disease, and polycystic ovary disease; in treating various types of cancer such as thyroid cancer, hepatome, leukemia, meningioma and conditions associated with cancer such as cancer cachexia; in the treatment of such conditions as hypotension such as orthostatic hypotension and postprandial hypotension and panic attacks; GH secreting adenomas (Acromegaly) and TSH secreting adenomas. Activation of type 2 but not type 5 subtype receptor has been associated with treating prolactin secreting adenomas. Other indications associated with activation of the somatostatin subtypes are inhibition of insulin and/or glucagon and more particularly diabetes mellitus, hyperlipidemia, insulin insensitivity, Syndrome X, angiopathy, proliferative retinopathy, dawn phenomenon and Nephropathy; inhibition of gastric acid secretion and more particularly peptic ulcers, enterocutaneous and pancreaticocutaneous fistula, Dumping syndrome, watery diarrhea syndrome, acute or chronic pancreatitis and gastrointestinal hormone secreting tumors; inhibition of angiogenesis, treatment of inflammatory disorders such as arthritis; chronic allograft rejection; angioplasty; preventing graft vessel and gastrointestinal bleeding. Somatostatin agonists can also be used for decreasing body weight in a patient. Accordingly, the compounds of the instant invention are useful for the foregoing methods.
Accordingly, the present invention includes within its scope pharmaceutical compositions comprising, as an active ingredient at least one of the compounds of Formula (I) or (II) in association with a pharmaceutically acceptable carrier.
The compounds of this invention can be administered by oral, parenteral (e.g., intramuscular, intraperitoneal, intravenous or subcutaneous injection, or implant), nasal, vaginal, rectal, sublingual or topical routes of administration and can be formulated with pharmaceutically acceptable carriers to provide dosage forms appropriate for each route of administration.
Solid dosage forms for oral administration include capsules, tablets, pills, powders and granules. In such solid dosage forms, the active compound is admixed with at least one inert pharmaceutically acceptable carrier such as sucrose, lactose, or starch. Such dosage forms can also comprise, as is normal practice, additional substances other than such inert diluents, e.q., lubricating agent such as magnesium stearate. In the case of capsules, tablets and pills, the dosage forms may also comprise buffering agents. Tablets and pills can additionally be prepared with enteric coatings.
Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, the elixirs containing inert diluents commonly used in the art, such as water. Besides such inert diluents, compositions can also include adjuvants, such as wetting agents, emulsifying and suspending agents, and sweetening, flavoring and perfuming agents.
Preparations according to this invention for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, or emulsions. Examples of non-aqueous solvents or vehicles are propylene glycol, polyethylene glycol, vegetable oils, such as olive oil and corn oil, gelatin, and injectable organic esters such as ethyl oleate. Such dosage forms may also contain adjuvants such as preserving, wetting, emulsifying, and dispersing agents. They may be sterilized by, for example, filtration through a bacteria-retaining filter, by incorporating sterilizing agents into the compositions, by irradiating the compositions, or by heating the compositions. They can also be manufactured in the form_of sterile solid compositions which can be dissolved in sterile water, or some other sterile injectable medium immediately before use.
Compositions for rectal or vaginal administration are preferably suppositories which may contain, in addition to the active substance, excipients such as coca butter or a suppository wax.
Compositions for nasal or sublingual administration are also prepared with standard excipients well known in the art.
Further, a compound of this invention can be administered in a sustained release composition such as he described in the following patents. U.S. Pat. No. 5,672,659 teaches sustained release compositions comprising a bioactive agent and a polyester. U.S. Pat. No 5,595,760 teaches sustained release compositions comprising a bioactive agent in a gelable form. U.S. application Ser. No. 08/929,363 filed Sep. 9, 1997, teaches polymeric sustained release compositions comprising a bioactive agent and chitosan. U.S. application Ser. No. 08/740,778 filed Nov. 1, 1996, teaches sustained release compositions comprising a bioactive agent and cyclodextrin. U.S. application Ser. No. 09/015,394 filed Jan. 29, 1998, teaches a absorbable sustained release compositions of a bioactive agent. The teachings of the foregoing patents and applications are incorporated herein by reference.
In general, an effective dosage of active ingredient in the compositions of this invention may be varied; it is necessary that the amount of the active ingredient be such that a suitable dosage form is obtained. The selected dosage depends upon the desired therapeutic effect, on the route of administration, and on the duration of the treatment, all of which are within the realm of knowledge of one of ordinary skill in the art. Generally, dosage levels of between 0.0001 to 100 mg/kg of body weight daily are administered to humans and other animals, e.g., mammals.
A preferred dosage range is 0.01 to 10.0 mg/kg of body weight daily, which can be administered as a single dose or divided into multiple doses.
Compounds of the instant invention can be and were assessed for its ability to bind to a somatostatin subtype receptor according to the following assays.
Human Somatostatin Subtype Receptor Binding Studies:
The affinity of a compound for human somatostatin subtype receptors 1 to 5 (sst1, sst2, sst3, sst4 and sst5, respectively) is determined by measuring the inhibition of [125I-Tyr11]SRIF-14 binding to CHO-K1 transfected cells.
The human sst1 receptor gene was cloned as a genomic fragment. A 1.5 Kb PstI-XmnI segment containing 100 bp of the 5′-untranslated region, 1.17 Kb of the entire coding region, and 230 bp of the 3′-untranslated region was modified by the Bg1II linker addition. The resulting DNA fragment was subcloned into the BamHI site of a pCMV-81 to produce the mammalian expression plasmid (provided by Dr. Graeme Bell, Univ. Chicago). A clonal cell line stably expressing the sst1 receptor was obtained by transfection into CHO-K1 cells (ATCC) using the calcium phosphate co-precipitation method (1). The plasmid pRSV-neo (ATCC) was included as a selectable marker. Clonal cell lines were selected in RPMI 1640 media containing 0.5 mg/ml of G418 (Gibco), ring cloned, and expanded into culture.
The human sst2 somatostatin receptor gene, isolated as a 1.7 Kb BamHI-HindIII genomic DNA fragment and subcloned into the plasmid vector pGEM3Z (Promega), was kindly by Dr. G. Bell (Univ. of Chicago). The mammalian cell expression vector is constructed by inserting the 1.7 Kb BamH1-HindII fragment into compatible restriction endonuclease sites in the plasmid pCMV5. A clonal cell line is obtained by transfection into CHO-K1 cells using the calcium phosphate co-precipitation method. The plasmid pRSV-neo is included as a selectable marker.
The human sst3 was isolated at genomic fragment, and the complete coding sequence was within a 2.4 Kb BamHI/HindIII fragment. The mammalian expression plasmid, pCMV-h3 was constructed by inserting the a 2.0 Kb NcoI-HindIII fragment into the EcoR1 site of the pCMV vector after modification of the ends and addition of EcoR1 linkers. A clonal cell line stably expressing the sst3 receptor was obtained by transfection into CHO-K1 cells (ATCC) using the calcium phosphate co-precipitation method. The plasmid pRSV-neo (ATCC) was included as a selectable marker. Clonal cell lines were selected in RPMI 1640 media containing 0.5 mg/ml of G418 (Gibco), ring cloned, and expanded into culture.
The human sst4 receptor or expression plasmid, pCMV-HX was provided by Dr. Graeme Bell (Univ. Chicago). The vector contains the 1.4 Kb NheI-NheI genomic fragment encoding the human sst4, 456 bp of the 5′-untranslated region and 200 bp of the 3′-untranslated region, clone into the XbaI/EcoR1 sites of PCMV-HX. A clonal cell line stably expressing the sst4 receptor was obtained by transfection into CHO-K1 cells (ATCC) using the calcium phosphate co-precipitation method. The plasmid pRSV-neo (ATCC) was included as a selectable marker. Clonal cell lines were selected in RPMI 1640 media containing 0.5 mg/ml of G418 (Gibco), ring cloned, and expanded into culture.
The human sst5 gene was obtained by PCR using a λ genomic clone as a template, and kindly provided by Dr. Graeme Bell (Univ. Chicago). The resulting 1.2 Kb PCR fragment contained 21 base pairs of the 5′-untranslated region, the full coding region, and 55 bp of the 3′-untranslated region. The clone was inserted into EcoR1 site of the plasmid pBSSK(+). The insert was recovered as a 1.2 Kb HindIII-XbaI fragment for subcloning into pCVM5 mammalian expression vector. A clonal cell line stably expressing the sst5 receptor was obtained by transfection into CHO-K1 cells (ATCC) using the calcium phosphate co-precipitation method. The plasmid pRSV-neo (ATCC) was included as a selectable marker. Clonal cell lines were selected in RPMI 1640 media containing 0.5 mg/ml of G418 (Gibco), ring cloned, and expanded into culture. CHO-K1 cells stablie expressing one of the human sst receptor are grown in RPMI 1640 containing 10% fetal calf serum and 0.4 mg/ml geneticin. Cells are collected with 0.5 mM EDTA, and centrifuged at 500 g for about 5 min. at about 4° C. The pellet is resuspended in 50 mM Tris, pH 7.4 and centrifuged twice at 500 g for about 5 min. at about 4° C. The cells are lysed by sonication and centrifuged at 39000 g for about 10 min. at about 4° C. The pellet is resuspended in the same buffer and centrifuged at 50000 g for about 10 min. at about 4° C. and membranes in resulting pellet are stored at −80° C.
Competitive inhibition experiments of [125I-Tyr11]SRIF-14 binding are run in duplicate in polypropylene 96 well plates. Cell membranes (10 μg protein/well) are incubated with [125I-Tyr11]SRIF-14 (0.05 nM) for about 60 min. at about 37° C. in 50 mM HEPES (pH 7.4), 0.2% BSA, 5 mM MgCl2, 200 KIU/ml Trasylol, 0.02 mg/ml bacitracin and 0.02 mg/ml phenylmethylsulphonylfluoride.
Bound from free [125I-Tyr11]SRIF-14 is separated by immediate filtration through GF/C glass fiber filter plate (Unifilter, Packard) presoaked with 0.1% polyethylenimine (P.E.I.). using Filtermate 196 (Packard) cell harvester. Filters are washed with 50 mM HEPES at about 0-4° C. for about 4 sec. and assayed for radioactivity using Packard Top Count.
Specific binding is obtained by subtracting nonspecific binding (determined in the presence of 0.1 μM SRIF-14) from total binding. Binding data are analyzed by computer-assisted nonlinear regression analysis (MDL) and inhibition constant (Ki) values are determined.
The determination of whether a compound of the instant invention is an agonist or an antagonist is determined by the following assay.
Functional Assay: Inhibition of cAMP Intracellular Production:
CHO-K1 Cells expressing human somatostatin (SRIF-14) subtype receptors are seeded in 24-well tissue culture multidishes in RPMI 1640 media with 10% FCS and 0.4 mg/ml geneticin. The medium is changed the day before the experiment.
Cells at 105 cells/well are washed 2 times by 0.5 ml and fresh RPMI with 0.2% BSA supplemented with 0.5 mM (1) 3-isobutyl-1-methylxanthine (IBMX) and incubated for about 5 min at about 37° C.
The reaction medium is removed and 200 ml 0.1 N HCl is added. cAMP is measured using radioimmunoassay method (Kit FlashPlate SMP001A, New England Nuclear).
The compounds of the instant invention are synthesized according to the following procedures and examples.
Gene Procedure: Two different methods can be applied; starting either from a carboxylic acid or an arylketone.
First method: Starting from a carboxylic acid (Macholan, L.; Skursky, L., Chem listy, 1955, 49, 1385-1388. Bestman, H. J., Seng, F., Chem. Ber., 1963, 96, 465-469).
A carboxylic acid is first converted into an acyl chloride using oxalyl chloride or thionyl chloride or activated as a mixed anhydride with an alkylchloroformate (isobutylchloroformate (Krantz, A., Copp, L. J., Biochemistry, 1991, 30, 4678-4687) or ethylchloroformate (Podlech, J., Seebach, D., Liebigs Ann., 1995, 1217-1228)) in the presence of a base (triethylamine or N-methyl morpholine).
The activated carboxyl group is then transformed into a diazoketone using ethereal diazomethane or trimethylsilyldiazomethane (Aoyama. T., Shiori. T., Chem. Pharm. Bull., 1981, 29, 3249-3255) in an aprotic solvent such as dietyl ether, tetrahydrofuran or acetonitrile.
The bromination is then carried out using a brominating agent such as HBr in acetic acid, hydrobromic acid in water or in diethyl ether.
To a solution of chloro-4-phenoxy-2-isobutyric acid (2.15 g, 10 mmol) in 10 ml of anhydrous dichloromethane at about 0° C. were added oxalyl chloride (5.5 ml, 11 mmol of a 2M solution in dichloromethane) and DMF (2 drops, catalytic amount) via a septum under nitrogen atmosphere. The solution was stirred and allowed to warm up to room temperature over about 3 hrs. Concentration under reduced pressure afforded the crude acid chloride which was used directly without further purification.
The acylchloride was added dropwise at about 0° C. to a solution of TMSCHN2 (11 ml, 22 mmol) in THF-acetonitrile (1:1, 10 ml). The mixture was stirred at about 25° C. for about 1 hour and then evaporated in vacuo.
A solution of the diazoketone in dichloromethane (10 ml) was added dropwise during about 10 minutes to a vigorously stirred mixture of concentrated hydrobromic acid (5 ml) in dichloromethane (20 ml). Nitrogen was evolved and a slight temperature rise occurred. After stirring for about a further 10 min., the mixture was diluted and the organic layer was washed with water (3 times 20 ml), dried over magnesium sulfate and evaporated. Flash chromatography of the residue eluting with AcOEt/Heptane (1:4) afforded the desired product with a yield of 79% (2.3 g).
1H-NMR in CDCl3 (100 MHz) δ: 7.05 (m, 4H, arom, H), 4.41 (s, 2H, CH2) 1.53 (s, 6H, 2 CH3).
The following compounds were prepared analogously to the procedure described for Preparation 1:
A methyl ketone is converted to a bromoketone by using different brominating agents:
To a solution of 3,4,5-trimethoxyacetophenone (2.1 g, 10 mmol) in methanol (30 ml) was added pyridine hydrobromide perbromide polymer (1.4 eq). The resulting mixture was shaken at room temperature for about 2 hours and the reaction was stopped by filtration. The polymer was washed with methanol and the filtrate was evaporated in vacuo. The product was then purified by flash chromatography (AcOEt/Heptane, 1:4) affording 1.5 g (53% ) of a white solid.
1H-NMR in CDCl3 (100 MHz) δ: 7.2 (s, 2H, H arom.), 4.4 (s, 2H, CH2) 3.9 (m, 9H, 3 OCH3).
The following compounds were prepared analogously to the procedure described for Preparation 7:
General Procedure: An amino acid is transformed to its cesium salt using cesium carbonate in a polar solvent such as DMF/H2O (1:1) or EtOH/H2O (1:1). An ester is then obtained using an appropriate bromoketone in a polar aprotic solvent such as dry DMF. The cesium bromide formed is filtered off and ammonium acetate is added in an aprotic solvent having a high boiling point such as xylene or toluene or in a protic acidic solvent such as acetic acid. The mixture is refluxed using a Dean-Stark trap for about 0.5-10 hours. In the scheme immediately below, PG is a protecting group, preferably a carbamate, such as t-Boc or benzyl carbamate.
A solution of Boc-(D,L)-Trp-OH (10 g, 32.8 mmol) and cesium carbonate (0.5 eq., 5.34 g) in EtOH/H2O (1:1, 70 ml) was shaken for about 30 minutes at room temperature, and then concentrated in vacuo about 40° C.
To the resulting salt in 40 mL of dry DMF was added 40 ml of a solution of 2-bromo-2′-methoxyacetophenone (7.66 g, 1 eq.) in dry DMF. The mixture was stirred for about 1 hr at room temperature under argon and then concentrated under reduced pressure. Ethyl acetate was added (100 ml), the mixture filtered, and the CsBr washed with ethyl acetate. The filtrate was then concentrated under reduced pressure.
A solution of the foregoing filtrate and ammonium acetate (50.5 g, 20 eq.) in xylene (240 ml) was refluxed for about 3 hours at about 150° C. Excess NH4OAc and H2O were removed using a Dean-Stark trap. The progress of the reaction was monitored by t.l.c. (eluent: CH2Cl2:MeOH, 95:5). The mixture was then concentrated under reduced pressure. The resulting residue was dissolved in ethyl acetate (100 ml) and washed with saturated aqueous NaHCO3 solution until basic pH, and with brine until neutral pH. The organic layer was then dried over MgSO4, and concentrated under reduced pressure.
Purification of the resulting residue by flash chromatography (eluent: CH2Cl2:MeOH, 95:5) afforded the desired compound (8.7 g, yield: 61%).
1H-NMR (CDCl3, 100 MHz) δ: 8.00 (s, 1H, NH), 7.80 (m, 2H, arom, H), 7.20 (m, 9H, arom, H, NH), 5.40 (m, 1H, NH), 5.10 (m, 1H, CH), 3.80 (s, 3H, OCH3), 3.50 (m, 2H, CH2), 1.50 (s, 9H, 6 CH3). LC/MS: m/z=433.3 (M+H).
A solution of the 2-{2-[(1S)-1-(tertbutoxycarbonylamino)-2-(indol-3-yl)ethyl]-1H-imidazol-4-yl}-2-methyl-propionic acid-methyl ester 1 (2.6 g, 6 mmol), (prepared according to the procedure described in Example 1) and LiOH.H2O (1.7 g, 6.6 eq.) in THF (50 ml) were stirred at about 80° C. for about 3 hours. The progress of the reaction was monitored by t.l.c (CH2Cl2:MeOH, 95:5). The resulting mixture was then concentrated in vacuo. About 50 ml of water was added to the residue which was then acidified with glacial acetic acid until about pH 5. The product of the reaction was then extracted with ethyl acetate (3×50 ml) and washed with brine until neutral pH. The organic layer was dried with MgSO4, and concentrated under reduced pressure. The resulting intermediate 2 was obtained after crystallization in diethyl ether with a yield of 80% (2 g). 1H-NMR (400 MHz, DMSO) δ: 10.9 (s, 1H, NH), 7.1 (m, 7H, arom, H, NH), 5.00 (m, 1H, CH), 3.3 (m, 2H, CH2), 1.3 (m, 15H, 5 CH3). LC/MS: m/z=525.1 (M+TFA), m/z=413.2 (M+H).
The 2-{2-[(1S)-1-(tertbutoxycarbonylamino)-2-[(1H)-indol-3-yl]ethyl]-1H-imidazol-4-yl}-2-methyl-propionic acid 2 can be activated preferentially by carbonyldiimidazole in an aprotic solvent such as THF or DMF at about 20-100° C. for about 1-4 hours.
A solution of the acid 2 (1 g, 2.4 mmol) and carbonyldiimidazole (0.39 g, 2.4 mmol) in dry THF (20 ml) was shaken for about 1 hour at room temperature (25° C.).
N-Boc-ethylene-diamine (0.43 g, 2.7 mmol) was added and the mixture was shaken for about 1 hour at about 25° C.
The mixture was diluted in ethyl acetate (100 ml) and washed with saturated aqueous NaHCO3 solution (2×50 ml) and brine until neutral pH. The organic layer was then dried over MgSO4, and concentrated in vacuo.
Purification of the resulting residue by flash-chromatography (in CH2Cl2:MeOH, 95:5) afforded the desired product 3 with a yield of 77% (1 g).
1H-NMR (400 MHz, DMSO) δ: 11.6 (s, 1H, NH), 10.7 (s, 1H, NH), 7.00 (m, 9H, arom, H, NH), 4.8 (m, 1H, CH), 3.00 (m, 6H, 3 CH2), 1.3 (m, 24H, 8 CH3). LC/MS: m/z=667.3 (M+TFA), 555.3 (M+H).
The following compounds were prepared analogously to the procedure described for Example 1 or 2 using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R3, R5 shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (PG (2 substituents)R3 (12 substituents)(R5 (49 substituents))=1176.
PG can also be hydrogen in the foregoing formula,
General procedure: Carboxylic acids are activated overnight at room temperature with carbonyldiimidazole in an aprotic solvent such as chloroform, THF or THF/DMF before addition of an amino starting material as shown above followed by a further 12-15 hours of stirring. The excess acylating agent is quenched with aminomethylated resin for about 12-15 hours and then purified on silica gel pad with dichloromethane or ethyl acetate as eluent.
For protected basic derivatives (R3=(CH2)4NHBoc and/or X2 containing NHBoc group), the corresponding deprotected compounds were obtained after treatment under acidic condition (DCM/TFA 10%) to remove the Boc group.
2-Furancarboxylic acid (12.6 mg, 0.11 mmol) was activated overnight at about 22° C. with carbonyldiimidazole (0.11 mmol, 0.2M in chloroform). 2-{(1S)-1-Amino-2-[indol-3-yl]ethyl}-4-phenyl-1H-imidazole (0.1 mmol, 0.5M in chloroform) was added to the media and the mixture was stirred for about 12 hours at about 22° C. Aminomethylated resin was then added (50-60 mg, 1.2 mmol/g, Novabiochem) in order to quench the excess of acylating agent for about 12 hours. Purification on silica gel pad (200 mg, Alltech) with ethyl acetate as eluent gave the expected product (37.2 mg, 94%). 1H-NMR (CDCl3, 100 MHz) δ: 8.36 (br s, 1H); 7.67-6.4 (m, 16H); 5.48 (qd, J=7.1 Hz, 1H); 3.6 (ABX system, 2H). LC/MS: m/z=397 (M+H).
The following compounds were prepared analogously to the procedure described for Example 1179 using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R3, R5 and X2, shown below were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R3 (4 substituents))(R5 (7 substituents))(X2 (87 substituents))=2436.
From Isocyanates and Isothiocyanates:
General procedure: Isocyanates or isothiocyanates are shaken overnight at room temperature with an imidazoyl intermediate in a aprotic solvent like dichloromethane, chloroform or chloroform/DMF. The reaction is quenched by addition of aminomethylated for about 12-15 hours and purified on silica gel pad with ethyl acetate as eluent.
For protected basic derivatives (R3=(CN2)4NHBoc), the corresponding deprotected compounds were obtained after treatment under acidic condition (DCM/TFA 10%) to remove the Boc group.
2,6-Difluorophenylisocyanate (36 μl, 0.3 mmol) and 2-{(1R)-1-amino-2-[indol-3-yl]ethyl}-4-phenyl-1H-imidazole (60.4 mg, 0.2 mmol) were stirred overnight in 2 mL of anhydrous dichloromethane. Filtration and purification by flash chromatography on silica gel (ethyl acetate/heptane 1:1 as eluent) afforded the expected product as a white powder (27 mg, 30%). 1H-NMR (DMSO D6, 400 MHz) δ: 12.03 (s, 1H); 10.77 (s, 1H); 8.47 (s, 1H); 8.1 (dd, 1H); 7.8-6.92 (m, 14H); 5.11 (dd, J=7 and 14 Hz, 1H); 3.3 (m, 2H). LC/MS: m/z=458 (M+H).
The following compounds were prepared analogously to the procedure described for Example 3616, using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R3, R5, and X2 with Y is O or X2 with Y is S, shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R3 (3 substituents))(R5 (7 substituents))(X2 (39 substituents))=819.
From Carbamate Intermediates and Primary and Secondary Amines:
General Procedure: The preparation of carbamate intermediates is described in the literature (Takeda. K. et al., Tetrahedron Letters 1983, 24, 4569-4572; Nimura, N. et al., Anal. Chem. 1986, 58, 2372-2375) from amino derivatives and N,N′-disuccinimidylcarbonate in acetonitrile at room temperature.
302.4 mg (1 mmol) of 2-{(1R)-1-amino-2-[indol-3-yl]ethyl}-4-phenyl-1H-imidazole previously dissolved in 20 mL of anhydrous acetonitrile was added dropwise to a solution of N,N′-disuccinimidylcarbonate (528 mg, 2 mmol, DSC) in 20 mL of anhydrous acetonitrile during 1.5 hour. After a further 4 hours of stirring at room temperature, the solvent was evaporated in vacuo and the residue redissolved in 30 mL of chloroform. Excess DSC was then discarded and the organic layer washed with water (4×30 mL), dried over MgSO4 and concentrated to obtain a brown solid (215 mg, 49%). 1H-NMR (CDCl3, 100 MHz) δ: 8.22 (brs, 1H); 8.1-7.08 (m, 12H); 5.9 (br s, 1H); 4.97 (dd, J=3.6 and 9.3 Hz, 1H); 3.75 (dd, J=3.6 and 14.8 Hz, 1H), 3.06 (dd, J=9.7 and 14.6 Hz, 1H); 2.96 (s, 2H); 2.89 (s, 2H). LC/MS: m/z=329 ((M+H)−SuOH.
General procedure: A primary or secondary amine is stirred for about 2-15 hours at room temperature with a carbamate intermediate in an aprotic solvent like acetonitrile. Tetrahydrofuran and aminomethylated resin are then added and the reaction is then stirred for about 12-15 hours. Ureas are isolated after filtration, rinsed with ethyl acetate and evaporated in vacuo.
For protected basic derivatives (R3=(CH2)4NHBoc), the corresponding deprotected compounds were obtained after treatment under acidic condition (DCM/TFA 10%) to remove the Boc group.
Benzylamine (5 μL, 50 mmol) and 2-{(1R)-1-amino-2-[indol-3-yl]ethyl}-4-phenyl-1H-imidazole (24 mg, 54 mmol) were stirred for about 2 hours at room temperature in anhydrous acetonitrile. Aminomethylated resin (50 mg, 0.75 mmol/g, Novabiochem) was then added and after further stirring overnight, the title product was obtained by filtration on silica gel pad (200 mg) and evaporated in vacuo as a brown powder (20 mg, 92%). 1H-NMR (DMSO D6, 100 MHz) δ: 10.6 (br s, 1H); 7.9-6.88 (m, 17H); 6.53 (m, 2H); 5.12 (dd, J=6 and 14.6 Hz, 1H); 4.28 (m, 2H); 3.25 (m, 2H). LC/MS: m/z=436 (M+H).
The following compounds were prepared analogously to the procedure described for Example 4437, using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R3, R5 and NX1X2, shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R3 (3 substituents))(R5 (12 substituents))(NX1X2 (112 substituents))=4032.
(Kaldor, S. W.; Siegel, M. G.; Fritz, J. E.; Dressman, B. A.; Hahn., P. J. Tetrahedron Letters 1996, 37, 7193-7196)
General procedure: Condensation of aldehydes with an imidazolyl intermediate in a protic solvent like methanol yields imines which are reduced in presence of AMBERLITE® IRA-400 borohydride. The slurry is then shaken overnight and the excess amino intermediate is quenched by addition of dichloromethane and aldehyde Wang resin. After further overnight stirring, the mixture is filtered, evaporated and purified on silica gel pad with ethyl acetate as eluent.
For protected basic derivatives (R3=(CH2)4NHBoc), the corresponding deprotected compounds were obtained after treatment under acidic condition (DCM/TFA 10%) to remove the Boc group.
2-{(1R)-1-Amino-2-[indol-3-yl]ethyl}-4-phenyl-1H-imidazole (36.3 mg, 0.12 mmol) and p-anisaldehyde (12 μl, 0.1 mmol) in 1 mL of methanol were shaken for about 2 hours at about 22° C. Borohydride resin (76 mg, 2.5 mmol/g, AMBERLITE® IRA-400) was then added and the slurry was stirred overnight before addition of dichloromethane (1 mL) and aldehyde Wang resin (31 mg, 3.22 mmol/g, Novabiochem). After about 8 hours of stirring, the slurry was then filtered and evaporated in vacuo to give a yellow solid (32.2 mg. 76%). 1H-NMR (CDCl3, 100 MHz) δ: 8.88 (br s, 1H); 7.73-6.68 (m, 15H); 4.62 (s, 1H); 4.33 (dd, J=4.7 and 8.5 Hz, 1H); 3.81 (s, 2H); 3.74 (s, 3H); 3.27 (ABX system, 2H)); 2.26 (s, 1H). LC/MS: m/z=423 (M+H).
The following compounds were prepared analogously to the procedure described for Example 8470, using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R3, R5 and A1, shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R3 (3 substituents))(R5 (7 substituents))(X2 (41 substituents))=861.
A series of thioimidates were previously synthesized by condensation of thioamides and iodomethane in acetone at room temperature. The precipitate was collected and then rinsed with acetone. Thioimidates so formed were used without further purification.
General procedure: Thioimidates are stirred overnight at room temperature with an amino intermediate in 2-propanol or 2-propanol/DMF before addition of tetrahydrofuran and aminomethylated resin. Further stirring overnight followed by filtration and washing with ethyl acetate yields an iodohydrate amidine after evaporation in vacuo.
For protected basic derivatives (R3=(CH2)4NHBoc), the corresponding deprotected compounds were obtained after treatment under acidic condition (DCM/TFA 10%) to remove the Boc group.
2-{(1R)-1-Amino-2-[indol-3-yl]ethyl}-4-phenyl-1H-imidazole (15.1 mg, 0.05 mmol) and S-methyl-2-thiophenethiocarboximide hydroiodide (13 mg, 0.06 mmol) were shaken in 1 mL of 2-propanol for about 16 hours. Aminomethylated resin (50 mg, 1.31 mmol/g, Novabiochem) was then added and after further stirring overnight a brown solid (19.8 mg, 84%) was isolated by filtration and evaporation in vacuo. 1H-NMR (MeOD, 100 MHz) δ: 8.15 (m, 1H); 7.84-6.96 (m, 13H); 5.3 (m, 1H); 3.61 (m, 2H). LC/MS: m/z=412 (M+H).
The following compounds were prepared analogously to the procedure described for Example 9332, using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R3, R5 and X2, shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R3 (7 substituents))(R5 (7 substituents))(X2 (12 substituents))=588.
The following compounds were prepared analogously to the procedure described for Example 9332, using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to known to those skilled in the art or as enabled by the teachings herein. Each combination of R4 and X7, shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R4 (2 substituents))(X7 (3 substituents))=6.
General procedure: A solution of an imidazole intermediate, and alkylating agent such as an α-bromoketone, an α-bromoester, an aryl or alkyl bromide or a sulfonyl chloride, in the presence of an organic or non-organic base which can be or not be supported on a resin such as polystyrene resin, in an aprotic solvent like THF, CH3CN, DMF is heated at 20-80° C. for 2-48 hours. The resulting N-alkylated compound can be isolated either by aqueous work-up followed by flash chromatography on silica gel, or by addition to the reaction mixture of a nucleophile supported on polymer (to trap the excess of electrophile) such as aminomethyl or thiomethyl polystyrene resin followed by filtration and then rapid purification of the resulting residue on a silica gel pad (using Alltech silica cartridge and Alltech manifold).
To a solution of 2-[1(S)-{(1,1-dimethylethoxy)carbonylamino}-2-phenylethyl]-4-phenyl-1H-imidazole (100 mg, 1 eq) in DMF (2 mL) were successively added morpholinomethyl polystyrene resin (Novabiochem, loading: 3.51 mmol/g, 159 mg, 2 eq) and 1-bromo-2-butanone (28 mL, 2 eq). After about 18 hours of stirring at about 20° C., 2 mL DMF were added to the reaction mixture followed by aminomethylpolystyrene resin (Novabiochem, loading: 1.73 mmol/g, 319 mg). The mixture was stirred overnight at 20° C. and filtered. The filtrate was concentrated under reduced pressure and then purified by a rapid filtration on a silica gel pad (Alltech silica cartridges) with ethylacetate as eluent to yield 107 mg (90% yield) of the title compound. NMR (1H, 400 MHz, CDCl3) δ: 7.80-6.98 (m, 11H, arom, H), 5.45 (d, 1H, NH), 4.80 (m, 1H, CH), 4.40 (AB, J=18 Hz, NCH2CO), 3.33 (m, 2H, CH2Ph), 2.25 (m, 2H, CH2CH3), 1.0 (t, 3H, CH3). LC/MS: calculated MW=433.5, m/z=434.2 (M+H), m/z=432.2 (M−H).
The following compounds were prepared analogously to the procedure described for Example 9927, using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R3, R5 and R1, shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R1 (34 substituents {see definitions of Z1}))R3 (5 substituents))(R5 (14 substituents))=2380.
General procedure: Intermediate (a) is treated with an acidic solution preferably TFA in DCM at about 20-30° C. for about 1-4 hours. The mixture is then concentrated under reduced pressure to afford a dihydro-imidazo-pyrazine.
A solution of 2-[1(S)-{1,1-dimethylethoxy)carbonylamino}-2-(3-indolyl)ethyl]-1-(benzoylmethyl)-4-phenyl-1H-imidazole (prepared as described previously) (100 mg) in a mixture of 10% TFA in DCM (1.3 mL) was stirred for about 3 hours at about 20° C. and concentrated under reduced pressure to yield the expected dihydro-imidazo-pyrazine (yield=95%). LC/MS: calculated MW: 402.19, m/z=403.2 (M+H).
The following compounds were prepared analogously to the procedure described for Example 12308, using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R5 and R7, shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R5 (7 substituents))(R7 (32 substituents))=224.
General procedure: Intermediate (b) is treated with an acidic solution preferably TFA in DCM at 20-30° C. for 14 hours. The mixture is then concentrated under reduced pressure to afford compound (c) which is oxidized to the corresponding fully aromatized imidazopyrazine either by keeping it in solution in methanol or DMSO for 5 hours-3 days at about 20° C. or by using an oxidative reagent such as manganese dioxide in a protic or aprotic solvent such as MeOH, toluene or chloroform at 20-70° C. for 2-10 hours or chromic acid supported or not on a resin in a protic solvent like methanol at 40-70° C. for 3-15 hours.
A solution of 2-[1,5-bis{(1,1-dimethylethoxy)carbonylamino}pentyl]-4-phenyl-1H-imidazole (50 mg) in a mixture of TFA/DCM 10% (700 mL) was stirred at about 20° C. for about 3 hours and then concentrated under reduced pressure to yield the intermediate dihydro-imidazo-pyrazine as its trifloroacetate salt. This salt was dissolved in MeOH (1 mL) and manganese dioxide (30 mg) was added. After about 3 hours of stirring at about 20° C., the mixture was filtered on a CELITE® pad and the filtrate concentrated under reduced pressure to afford the fully aromatized imidazo-pyrazine (78% yield). NMR (1H, 400 MHz CD3OD): 8.75-7.34 (m, 12H, arom, H), 3.32 (m, 4H, CH2), 2.10 (m, 2H, CH2), 1.90 (m, 2H, CH2). LC/MS: calculated MW=342.4, m/z=343.2 (M+H).
The following compounds were prepared analogously to the procedure described for Example 12533, using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein. Each combination of R3 and R7, shown below, were or can be synthesized, therefore, the number of Examples are calculated by multiplying (R3 (5 substituents))R5 (8 substituents))R7 (31 substituents))=1240.
General procedure: Intermediate (d) is treated with an acidic solution preferably TFA in DCM at 20-30° C. for 14 hours. The mixture is then concentrated under reduced pressure to afford the intermediate dihydro-imidazopyrazine (e). Reduction of (e) to the corresponding tetrahydro-imidazopyrazine is achieved by catalytic hydrogenation or by using any reducing agent such as NaBH4 (which can be supported on a resin), NaBH(OAc)3, NaBH3CN in a protic solvent such as MeOH at pH maintained weakly acidic (around pH 5) by addition of acetic acid or TFA.
2-[1(S)-{(1,1-Dimethylethoxy)carbonylamino}-2-phenylethyl]-1-(2-oxo-butyl)-4-phenyl-1H-imidazole (60 mg) in a mixture of 10% TFA in DCM was stirred at about 20° C. for about 3 hours and then concentrated under reduced pressure. The resulting intermediate dihydro-imidazo-pyrazine was dissolved in methanol and borohydride supported on resin (AMBERLITE® IRA 400, Aldrich, 2.5 mmol BH4−/g; 4 eq) was added. The pH was maintained at about 5 by addition of drops of TFA. After about 2 hours of stirring at about 20° C., the mixture was filtered and the filtrate concentrated under reduced pressure. The residue was purified by flash chromatography (ethyl acetate/Heptane 7:3; Rf=0.30). The tetrahydro-imidazo-pyrazine was obtained as a single diastereoisomer in 86% yield (38 mg) NMR (1H, 400 MHz, CDCl3) δ: 7.87-7.10 (m, 11H, arom, H), 4.28 (dd, 1H, 3J=10 Hz, 3J=3 Hz, H8), 3.95 (dd, 1H, 2J=11.5 Hz, 3J=3.6 Hz), 3.85 (dd, 1H, 2J=13.6 Hz, 3J=3.0 Hz), 3.60 (t, 1H, 2J=3J=11.5 Hz), 3.85 (dd, 1H, 2J=13.6 Hz, 3J=10, Hz), 2.98 (m, 2H), 1.85 (s, 1H, NH), 1.55 (m, 2H, CH2), 0.95 (t, 3H, CN3). NMR (13C, 100 MHz CDCl3): 146.3, 140.9, 138.0, 134.4, 129.4, 128.6, 128.5, 126.6, 126.5, 124.8, 113.8, 55.9, 54.4, 50.2, 40.0, 26.6, 10.0. LC/MS: calculated MW=317.43, m/z=318.20 (M+H).
The following compound was prepared analogously to the procedure described for Example 13774 using the appropriate starting materials, which can be obtained from commercial sources or synthesized according to methods known to those skilled in the art or as enabled by the teachings herein.
General procedure: A compound of formula (f) can react with isocyanates, isothiocyanates, N-succinimidyl carbamates, acyl chlorides or activated carboxylic acids in an aprotic solvent at 20-70° C. for 2-18 hours. The resulting derivative can be isolated by evaporation of the mixture followed by flash chromatography on silica gel or by addition to the mixture of a nucleophile supported on polymer such as aminomethyl or thiomethyl polystyrene resin followed by a filtration.
To a solution of 5,6,7,8-tetrahydro-2,6-diphenyl-8(S)-phenylmethyl-imidazo[1,2-a]pyrazine (29 mg) in chloroform were successively added morpholinomethylpolystyrene resin (Novabiochem, loading=3.51 mmol/g, 50 mg, 2 eq) and methoxyacetylchloride (10 mL, 1.3 eq). After about 3 hours of stirring at about 20° C. chloroform was added to the mixture followed by aminomethylpolystyrene resin (Novabiochem, loading=1.2 mmol/g, 132 mg, 2 eq). The reaction mixture was stirred for another 2 hours and then filtered. The filtrate was concentrated under reduced pressure to afford 23 mg of the title compound (yield=68%). NMR (1H, 100 MHz, CDCl3): 7.9-7.0 (m, 16H, arom, H), 6.6 (m, 1H, H1), 5.3 (m, 1H, H1), 4.6 (dd, 1H, 2J=13 Hz, H5), 4.35 (dd, 1H, 2J=13 Hz, 3J=5 Hz, H5′), 3.7-2.9 (m, 5H, CH2Ph, OCH3).
The following tables of compounds illustrate some of the compounds of the present invention that were synthesized and provide the hplc retention time (denoted Rt or Tr) in minutes and mass spectra results of each compound.
Mass spectra were acquired on a single quadrupole electrospray mass spectrometer (Micromass, Platform model), 0.8 Da resolution. A monthly calibration, between 80 and 1000 Da, is performed with sodium and rubidium iodide solution isopropanol/water (1/1 Vol.).
HPLC retention times were acquired on an HPLC system: HP1100 (Hewlett-Packard) equipped with a photodiode array UV detector.
The HPLC conditions are as follows and the conditions used for each of the following tables of compounds are noted below, the wavelength of the UV detector is noted in parenthesis after the formula number.
In the following description Formula numbers are noted in bold and the wavelength is in parenthesis.
13777.1) 2-((tert-butoxycarbonyl)amino)-6-methylheptanoic acid
A solution of diisopropylamine (13.2 ml; 0.094 mol) in 130 ml tetrahydrofurane (THF) was cooled down to about −40° C. n-Butyllithium (37 ml of a 2.5 M solution in hexane; 0.094 mol) was added dropwise. The temperature was left to return to about 0° C. At this temperature, Boc-glycine (5 g; 0.028 mol) in solution in 30 ml THF was introduced into the mixture. After ten minutes at this temperature, 1-bromo-4-methylpentane (7.9 ml; 0.056 mol) in solution in 20 ml THF was quickly added. The temperature was then left to return to about 23° C. and the mixture agitated for about one hour at this temperature. After hydrolysis with 100 ml water and acidification with 150 ml of a saturated potassium hydrogenosulfate solution, the obtained mixture was extracted with 2 times 50 ml ethyl acetate. The organic phase was washed with 100 ml water followed by 100 ml of a saturated sodium chloride solution. After drying on magnesium sulfate and evaporation of the solvent, the residue obtained was purified on a silica column (eluent: ethyl acetate-heptane/6-4) to produce a white-colored powder with a yield of 50%. MH+=260.3.
13777.2) tert-butyl 1-(4-(3-bromophenyl)-1H-imidazol-2-yl)-5-methylhexylcarbamate
A mixture of 2-((tert-butoxycarbonyl)amino)-6-methylheptanoic acid (3.5 g; 0.0135 mol) and cesium carbonate (4.89 g; 0.015 mol) in 100 ml ethanol was agitated at about 23° C. for about 1 hour. The ethanol was eliminated by evaporation under reduced pressure in a rotative evaporator. The mixture obtained was dissolved in 100 ml of dimethylformamide and 3-bromophenacyl bromide (3.75 g; 0.0135 mol) was then added. After about 16 hours agitation, the solvent was evaporated under reduced pressure. The mixture obtained was taken up in ethyl acetate and the cesium bromide was then filtered. The ethyl acetate of the filtrate was evaporated and the reaction oil was taken up in a mixture of xylene (100 ml) and ammonium acetate (46.2 g; 0.6 mol). The mixture was then heated to reflux for about one hour and a half and, after cooling, a mixture of icy water and ethyl acetate was poured in the reaction medium. After phase separation, the organic phase was washed with a sodium saturated bicarbonate solution, dried over magnesium sulfate and then evaporated under vacuum. The solid obtained was filtered and then washed with ether to produce a white powder (yield of 63%). Melting point: 134-136° C. MH+=436.2.
13777.3) 1-(4-(3-bromophenyl)-1H-imidazol-2-yl)-5-methyl-1-hexanamine
tert-Butyl 1-(4-(3-bromophenyl)-1H-imidazol-2-yl)-5-methylhexylcarbamate (obtained at stage 13777.2; 3.5 g; 0.008 mol) was agitated in 120 ml of an ethyl acetate solution saturated in hydrochloric acid for about 2.5 at a temperature of about 55° C. The solid obtained was filtered and washed with ether. A white powder was obtained with a yield of 97%. Melting point: 200-202° C. MH+=336.2.
13777.4) N-{1-(4-(3-bromophenyl)-1H-imidazol-2-yl)-5-methylhexyl}-N-cyclohexylamine
A mixture containing 1-(4-(3-bromophenyl)-1H-imidazol-2-yl)-5-methyl-1-hexanamine (obtained at stage 13777.3; 0.8 g; 0.0019 mol), triethylamine (0.4 ml; 0.003 mol) and cyclohexanone (0.32 ml; 0.0023 mol) in 10 ml methanol was agitated for about 30 minutes at about 23° C. Sodium triacetoxyborohydride (630 mg; 0.003 mol) was then added. The reaction mixture was agitated for about 16 hours and then poured into water. After extraction with ethyl acetate, the organic phase was washed with a saturated sodium chloride solution and then dried over magnesium sulfate. The solvent was evaporated and the residue purified over a silica column (eluent mixture CH2Cl2-MeOH/95-05). A white-colored powder was obtained with a yield of 38%. Melting point: 236-238° C. MH+=418.2.
13778.1) tert-butyl 1-(4-(4-fluorophenyl)-1H-imidazol-2-yl)heptylcarbamate
This compound was obtained according to a protocol analogous to that of stage 13777.2 of example 13777, using 2-((tert-butoxycarbonyl)amino)octanoic acid (6.2 g; 0.024 mol) instead of 2-((tert-butoxycarbonyl)amino)-6-methylheptanoic acid and 2-bromo-4-fluoroacetophenone (5.2 g; 0.024 mol) instead of 3-bromophenacyl bromide. A white powder was obtained (yield: 58%), which was sufficiently clean to be used as was for the following stage.
13778.2) 1-(4-(4-fluorophenyl)-1H-imidazol-2-yl)-1-heptanamine
This compound was obtained according to a protocol analogous to that of stage 13777.3 of example 13777, using tert-butyl 1-(4-(4-fluorophenyl)-1H-imidazol-2-yl)heptylcarbamate (5.2 g; 0.014 mol) as starting compound. After purification over a silica column (eluent: CH2Cl2-MeOH-NH4OH/89-10-1), a gray powder was obtained (yield of 72%). Melting point: 148-150° C. MH+=276.2.
13778.3) N-{1-(4-(4-fluorophenyl)-1H-imidazol-2-yl)heptyl}cyclohexanamine
This compound was obtained according to a protocol analogous to that of stage 13777.4 of example 13777, using 1-(4-(4-fluorophenyl)-1H-imidazol-2-yl)-1-heptanamine (0.5 g; 0.0014 mol) as starting amine and cyclohexanone (0.17 ml; 0.0014 mol) as starting ketone. A white powder was obtained with a yield of 15%. Melting point: 190-192° C. MH+=358.2.
Triethylamine (0.83 ml; 0.006 mol) was added at about 23° C. to a solution of (1R)-1-(1-benzyl-4-tert-butyl-1H-imidazol-2-yl)-2-(1H-indol-3-yl)ethanamine (0.7 g; 0.002 mol; prepared according to experimental conditions analogous to the preceding examples and using the appropriate starting compounds and reaction products) in 15 ml acetonitrile. The mixture was agitated about one hour at about 23° C. and benzyl chloride (0.23 ml; 0.002 mol) was added. Agitation was maintained for about 16 hours. The reaction mixture was concentrated using a rotative evaporator and the oil obtained was taken up in ethyl acetate and water. The aqueous phase was extracted with ethyl acetate and washed with water and then with a saturated solution of sodium chloride. The solvents were evaporated under vacuum. After purification over a silica column (eluent: ethyl acetate-heptane/7-3), a strong beige solid was obtained in the form of a glue (yield of 5%). Free base. Melting point: 60-62° C. MH+=463.3.
(R,S)-1-(4-phenyl-1H-imidazol-2-yl)heptylamine (1 g; 0.003 mol; prepared according to experimental conditions analogous to the preceding examples and using the appropriate starting compounds and reaction products) was diluted in 20 ml dimethylformamide. Potassium carbonate (2.2 g; 0.016 mol) was added at about 23° C. and then benzyl bromide (1.2 ml; 0.010 mol) was added quite slowly. The mixture was agitated about 72 hours at about 23° C. before being poured in icy water. The mixture was extracted with ethyl acetate. The organic phase was washed with water and then a saturated solution of sodium chloride. After drying over magnesium sulfate, the solvents were concentrated using a rotative evaporator. After purification over a silica column (eluent: ethyl acetate-heptane/10-90), a white powder was obtained (yield of 31%). Free base. Melting point 94-96° C. MH+=438.3.
N-benzyl(4-(1,1′-biphenyl)-4-yl-1H-imidazol-2-yl)methanamine (1 g; 0.0024 mol; prepared according to experimental conditions analogous to the preceding examples and using the appropriate starting compounds and reaction products) was diluted in 15 ml dimethylformamide. Potassium carbonate (1 g; 0.0073 mol) was added at about 23° C. and then hexane bromide (0.34 ml; 0.0024 mol) was added quite slowly. The reaction mixture was brought around the temperature of about 70° C. for about 3 hours before being poured in icy water. The mixture was extracted with ethyl acetate and the organic phase washed with water. After drying over magnesium sulfate, the solvents were concentrated using a rotative evaporator. After purification over a silica column (eluent: ethyl acetate-heptane/7-3), a light yellow solid was obtained in the form of a glue (yield of 13%). Free base. Melting point: 120-122° C. MH+=424.3.
(4-(1,1′-biphenyl)-4-yl-1H-imidazol-2-yl)-N-methylmethanamine (1 g; 0.003 mol; prepared according to experimental conditions analogous to the preceding examples and using the appropriate starting compounds and reaction products) was diluted in 20 ml dimethylformamide. Potassium carbonate (1.23 g; 0.009 mol) was added at about 23° C. and then benzyl bromide (0.34 ml; 0.003 mol) was added quite slowly. The reaction mixture was agitated at this temperature for about 48 hours then poured in icy water. The mixture was extracted with ethyl acetate and the organic phase washed with water. After drying over magnesium sulfate, the solvents were concentrated using a rotative evaporator. After purification over a silica column (eluent: ethyl acetate-heptane/8-2), a white solid was obtained in the form of a glue (yield of 16%). Free base. Melting point: 106-108° C. MH+=354.2.
(R,S)-1-(4-phenyl-1H-imidazol-2-yl)-1-heptanamine (1 g; 0.003 mol; prepared according to experimental conditions analogous to the preceding examples and using the appropriate starting compounds and reaction products) was diluted in 10 ml methanol. Triethylamine (0.9 ml; 0.006 mol) was added dropwise and the mixture was agitated for about 30 minutes at about 23° C. Hexanal (0.45 ml; 0.0036 mol) was then added and the mixture was agitated for about one hour at about 23° C. Sodium triacetoxyborohydride (1.3 g; 0.006 mol) was finally added. After about two hours agitation at about 23° C., water was added and the reaction mixture extracted with ethyl acetate. The organic phase was washed with water and dried over magnesium sulfate before evaporation of the solvents. After purification over a silica column (eluent: ethyl acetate-heptane/6-4), a chestnut solid was obtained in the form of a glue (yield of 3%). Free base. The melting point could not be measured (sticks). MH+=426.4.
(1R)-2-(1H-indol-3-yl)-1-(4-phenyl-1H-imidazol-2-yl)ethanamine (2 g; 0.0066 mol; prepared according to experimental conditions analogous to the preceding examples and using the appropriate starting compounds and reaction products) was diluted in 10 ml n-butanol. 2-bromopyrimidine (1 g; 0.0066 mol) and then diisoethylamine (1.15 ml; 0.0066 mol) were added dropwise. The mixture was then heated to around 80° C. for about 16 hours. The n-butanol was evaporated and the residue taken up in water and ethyl acetate. The organic phase was washed with water and then with a saturated solution of sodium chloride before being dried over magnesium sulfate and concentrated using a rotative evaporator. After purification over a silica column (eluent: ethyl acetate-heptane/7-3, followed by CH2Cl2-MeOH-NH4OH/95-4.5-0.5 and ethyl acetate). A white powder was obtained (yield of 20%). Free base. Melting point: 138-140° C. MH+=381.2.
(1R)-N-benzyl-2-(1H-indol-3-yl)-1-(4-phenyl-1H-imidazol-2-yl)ethanamine (0.5 g; 0.00127 mol; prepared according to experimental conditions analogous to that of example 38 using the appropriate starting compounds and reaction products) was diluted in 25 ml tetrahydrofurane. Methyl tosylate (0.24 g; 0.00127 mol) was added to the preceding at about 23° C. and then potassium tert-butylate (0.15 g; 0.00127 mol) was added quite slowly. Agitation at about 23° C. was maintained for about two hours and then the mixture was heated to around 60° C. for about 8 hours. The solvent was evaporated and the residue obtained taken up in ethyl acetate and a 10% sodium bicarbonate solution. After phase separation, the organic phase was washed with water and dried over magnesium sulfate. The solvent was then evaporated. After purification over a silica column (eluent: ethyl acetate-heptane/7-3), a light beige solid was obtained in the form of a glue (yield of 4%). Free base. Melting point: 110-112° C. MH+=407.3.
(1-benzyl-4-phenyl-1H-imidazol-2-yl)methanamine (0.6 g; 0.0018 mol; prepared according to experimental conditions analogous to the preceding examples and using the appropriate starting compounds and reaction products) was diluted in 15 ml tetrahydrofurane. Triethylamine (1.12 ml; 0.008 mol) and then methyl 4-toluenesulfonate (0.75 g; 0.004 mol) were added dropwise. The mixture was agitated about 48 hours at about 23° C. and then poured in icy water. After extraction with ether and phase separation, the organic phase was washed with water and afterwards with a saturated solution of sodium chloride. The organic phase was then dried over magnesium sulfate and concentrated using a rotative evaporator. After purification over a silica column (eluent: ethyl acetate-heptane/7-3 followed by CH2Cl2-MeOH/95-5), a white powder was obtained (yield of 44%). Free base. Melting point: 78-80° C. MH+=292.2.
The following further examples were made according to the procedures described in examples 13777 to 13786 and to the general procedures described in this application.
Free base. Melting point: 104-106° C.
Hydrochloride. Melting point: 228-230° C.
Hydrochloride. Melting point: 132-134° C.
Free base. Melting point: 102-104° C.
Hydrochloride. Melting point: 279-280° C.
Hydrochloride. Melting point: 150-152° C.
Free base. The melting point could not be measured (sticks).
Free base. Melting point: 98-100° C.
Free base. Melting point: 172-176° C.
Free base. Melting point: 201-203° C.
Free base. Melting point: 228-230° C.
Free base. The melting point could not be measured (sticks).
Free base. Melting point: 140-142° C.
Hydrochloride. Melting point: 146-148° C.
Hydrochloride. Melting point: starting from 115° C.
Free base. The melting point could not be measured (sticks).
Free base. The melting point could not be measured (sticks).
Hydrochloride. Melting point: 110-112° C.
Free base. The melting point could not be measured (sticks).
Free base. The melting point could not be measured (sticks).
Free base. The melting point could not be measured (sticks).
Free base. The melting point could not be measured (sticks).
Free base. The melting point could not be measured (sticks).
Free base. Melting point: 118-120° C.
Free base. Melting point: 68-70° C.
Free base. Melting point: 192-194° C.
Free base. Melting point 130-132° C.
Free base. The melting point could not be measured (sticks).
Hydrochloride. Melting point: 155-157° C.
Hydrochloride. Melting point: 144-146° C.
Free base. Melting point 100-102° C.
Hydrochloride. Melting point: 208-210° C.
Hydrochloride. Melting point: 180-182° C.
Hydrochloride. Melting point: 110-114° C.
Free base. Melting point 118-120° C.
Free base. Melting point: 120-122° C.
Free base. Melting point: 208-210° C.
Hydrochloride. The melting point could not be measured (sticks).
Free base. Melting point: 218-220° C.
Free base. Melting point: 105-108° C.
Free base. Melting point 220-222° C.
Free base. Melting point: 170-172° C.
Free base. Melting point: 140-142° C.
Free base. The melting point could not be measured (sticks).
Hydrochloride. Melting point: begins to stick around 220° C.
Hydrochloride. Melting point: 248-250° C.
Free base. Melting point: 94-96° C.
Hydrochloride. Melting point: 230-232° C.
Free base. Melting point: 60-62° C.
Free base. Melting point: 152-154° C.
Free base. Melting point: 208-210° C.
Hydrochloride. Melting point: 210-212° C.
Free base. Melting point: 88-90° C.
Free base. Melting point 134-135° C.
Free base. Melting point: 134-136° C.
Hydrochloride. The melting point could not be measured (sticks).
Free base. Melting point 72-74° C.
Hydrochloride. Melting point: 174-176° C.
Free base. Melting point 144-146° C.
Free base. Melting point: 142-144° C.
Free base. Melting point: 100-102° C.
Free base. The melting point could not be measured (sticks).
Free base. Melting point: 167-169° C.
Hydrochloride. Melting point: 240-242° C.
Hydrochloride. Melting point: 131-134° C.
Hydrochloride. Melting point: 170-174° C.
Free base. Melting point: 160-162° C.
Free base. Melting point 208-210° C.
Free base. Melting point: 96-100° C.
Free base. Melting point: 72-74° C.
Hydrochloride. Melting point: 206-210° C.
Free base. Melting point: 70-72° C.
Hydrochloride. Melting point: 218-220° C.
Hydrochloride. Melting point: 218-220° C.
Free-base. Melting point: 130-132° C.
Hydrochloride. Melting point: 215-218° C.
Hydrochloride. Melting point: >250° C.
Free base. Melting point: 210-213° C.
Free base. Melting point: 126° C.
Hydrochloride. Melting point: 197-200° C.
Hydrochloride. Melting point: 178-180° C.
Free base. Melting point: 77-80° C.
Free base. Melting point: 64-65° C.
Hydrochloride. Melting point: 157-160° C.
Hydrochloride. Melting point: 238-240° C.
Free base. Melting point: 200-202° C.
Free base. Melting point: 125-127° C.
Hydrochloride. Melting point: 182-184° C.
Free base. Melting point: 141-143° C.
Hydrochloride. Melting point: 231-232° C.
Hydrochloride. Melting point: 230-231° C.
Free base. Melting point: 142-144° C.
Acetate. Melting point: 115-116° C.
Free base. Melting point 138-140° C.
Free base. Melting point: 100-102° C.
Hydrochloride. Melting point: >250° C.
Free base. Melting point: 220-222° C.
Hydrochloride. Melting point: 185-188° C.
Free base. Melting point: 155-157° C.
Free base. Melting point 192-194° C.
Hydrochloride. Melting point: 218-220° C.
Free base. Melting point: starting from 126° C.
Free base. Melting point: 156-158° C.
Free base. Melting point: 145.6° C.
Hydrochloride. Melting point: 155.4° C.
Hydrochloride. Melting point: 204-206° C.
Hydrochloride. Melting point: 182-184° C.
Free base. Melting point: begins to stick around 130° C.
Free base. Melting point: 78.6° C.
Free base. Melting point: 218-220° C.
Free base. The melting point could not be measured (sticks).
Free base. Melting point 141-142° C.
Free base. Melting point: 188-189° C.
Hydrochloride. Melting point: 192° C.
Hydrochloride. Melting point: 178-181° C.
Hydrochloride. Melting point: 148-150° C.
Hydrochloride. Melting point: 138-140° C.
Free base. The melting point could not be measured (sticks).
Free base. The melting point could not be measured (sticks).
Free base. Melting point: 94-98° C.
Hydrochloride. Melting point: starting from 120° C.
Free base. Melting point: 126-128° C.
Hydrochloride. Melting point: starting from 110° C.
Hydrochloride. Melting point: starting from 90° C.
Hydrochloride. Melting point: 170° C.
Hydrochloride. Melting point: 148-150° C.
Free base. Melting point: 134-136° C.
Hydrochloride. Melting point: 200-202° C.
Acetate. Melting point 70-72° C.
Free base. Melting point: 92-94° C.
Free base. Oil.
Free base. Melting point: 148-150° C.
Free base. Melting point: 118-122° C.
Free base. Melting point: 114-116° C.
Free base. Melting point: 114-116° C.
Hydrochloride. Melting point: 194° C.
Hydrochloride. Melting point: 168-170° C.
Hydrochloride. Melting point: 220-222° C.
Free base. Melting point: 202-204° C.
Hydrochloride. Melting point: 180-190° C.
Hydrochloride. Melting point: 230-232° C.
Hydrochloride. Melting point: 142-144° C.
Hydrochloride. Melting point: 216.7° C.
Free base. Melting point: 224-226° C.
This application is a national phase application, under U.S.C. Section 371, of PCT/US01/23959 filed Jul. 31, 2001, which claims priority to U.S. Provisional Application No. 60/222,584 filed Aug. 1, 2000, the entire contents of which are incorporated herein by reference.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/US01/23959 | 7/31/2001 | WO | 00 | 10/20/2003 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO02/10140 | 2/7/2002 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 5128355 | Carini et al. | Jul 1992 | A |
| 5733882 | Carr et al. | Mar 1998 | A |
| 6852725 | Thurieau et al. | Feb 2005 | B1 |
| Number | Date | Country |
|---|---|---|
| 0 166 609 | Jan 1986 | EP |
| 2 132 632 | Nov 1972 | FR |
| 218 460 | Sep 1987 | HU |
| 61-024594 | Feb 1986 | JP |
| 01-203383 | Aug 1989 | JP |
| 10-502670 | Mar 1998 | JP |
| 10-504808 | May 1998 | JP |
| 9404494 | Mar 1994 | WO |
| 9508550 | Mar 1995 | WO |
| 9600730 | Jan 1996 | WO |
| WO 9611927 | Apr 1996 | WO |
| 9616040 | May 1996 | WO |
| 9724119 | Jul 1997 | WO |
| 9730053 | Aug 1997 | WO |
| 9743278 | Nov 1997 | WO |
| 9745425 | Dec 1997 | WO |
| 9827108 | Jun 1998 | WO |
| WO 9964401 | Dec 1999 | WO |
| WO 9965942 | Dec 1999 | WO |
| WO 0000477 | Jan 2000 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 20070032653 A1 | Feb 2007 | US |
| Number | Date | Country | |
|---|---|---|---|
| 60222584 | Aug 2000 | US |
