Claims
- 1. A process for preparing a microorganism immobilized on a carrier made of a polymer-coated cotton gauze comprising the following steps:
- (a) impregnating a sheet of cotton gauze having a mesh size of 10-50 with 20-50% weight of ethylene glycol methacrylate;
- (b) irradiating the impregnated gauze with ionizing radiation thereby to polymerize said ethylene glycol methacrylate to obtain a two-dimensional carrier made of a polymer-coated cotton gauze;
- (c) contacting said carrier with a liquid culture medium in which Trichoderma reesei cells can grow;
- (d) inoculating said medium with said cells to culture the cells on said polymer-coated cotton gauze; and
- (e) recovering a polymer-coated cotton gauze comprising the growing cells.
- 2. The process according to claim 1 wherein the ionizing radiation is selected from the group consisting of gamma rays and X rays, and the radiation is applied at a dosage rate of from 1.times.10.sup.4 to 1.times.10.sup.6 r/hr to give a total dosage of from 0.5 to 1 Mrad at a temperature ranging from 0 to 100.degree. C.
- 3. The process according to claim 1 wherein the temperature range is from ambient to 100.degree. C.
Priority Claims (1)
Number |
Date |
Country |
Kind |
59-134550 |
Jun 1984 |
JPX |
|
Parent Case Info
This application is a continuation-in-part of application Ser. No. 07/148,118, filed Jan. 19, 1988, now abandoned, which is a continuation-in-part of application Ser. No. 06/928,588, filed Nov. 10, 1988, now abandoned, which is a continuation of application Ser. No. 06/707,218, filed March 1, 1985, now abandoned.
US Referenced Citations (8)
Foreign Referenced Citations (3)
Number |
Date |
Country |
0122039 |
Jul 1983 |
JPX |
0042889 |
Mar 1984 |
JPX |
59-42889 |
Mar 1984 |
JPX |
Non-Patent Literature Citations (4)
Entry |
Chamber's Technical Dictionary, Third Edition, 1961, p. 27, The MacMillan Company. |
Encyclopedia of Chemical Technology, Third Edition, vol. 19, 1982, pp. 608-609, Kirk-Othmer. |
Hawley, The Condensed Chemical Dictionary, Van Nostrand Reinhold Company, 1977, pp. 741, 742. |
Birnbaum et al., Immobilized Cells, Solid Phase Biochemistry, John Wiley & Sons, N.Y., 1983, pp. 629 & 695-704. |
Continuations (1)
|
Number |
Date |
Country |
Parent |
707218 |
Mar 1985 |
|
Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
148118 |
Jan 1988 |
|
Parent |
928588 |
Nov 1986 |
|