Claims
- 1. An emmobnilized enzyme comprising an enzyme selected from the group consisting of penicillin-G amidase, glutaryl-7-ACA acylase and D-amino acid oxidase immobilized by covalent binding to an aminofunctional organosiloxane polymer carrier material having an average diameter of 0.01 to 3 mm wherein the enzyme has a specific volume activity of at least 100 U/g carrier material.
- 2. The immobilized enzyme as claimed in claim 1, wherein
- the enzyme is immobilized on the carrier material by means of a dialdehyde.
- 3. The immobilized enzyme as claimed in claim 1, wherein
- the weight ratio of enzyme to carrier material is in the range of 1 to 300 mg protein per g wet carrier.
- 4. The immobilized enzyme as claimed in claim 1, wherein
- the average particle size of the carrier material is 0.1 to 0.4 mm.
- 5. The immobilized enzyme as claimed in claim 1, wherein
- the carrier material is essentially spherical.
- 6. The immobilized enzyme as claimed in claim 1, wherein the enzyme is penicillin-G amidase and
- the weight ratio of enzyme to carrier material is in the range of 2 to 200 mg protein per g wet carrier.
- 7. The immobilized enzyme as claimed in claim 6, wherein
- the weight ratio of enzyme to carrier material is in the range of 40 to 80 mg protein per g wet carrier.
- 8. The immobilized enzyme as claimed in claim 6, wherein
- the specific volume activity is in the range of 100 to 300 U per g wet carrier.
- 9. The immobilized enzyme as claimed in claim 1, wherein the enzyme is glutaryl-7-ACA acylase and
- the weight ratio of enzyme to carrier material is in the range of 10 to 110 mg protein per g wet carrier.
- 10. The immobilized enzyme as claimed in claim 9, wherein
- the weight ratio of enzyme to carrier material is in the range of 20 to 70 mg protein per g wet carrier.
- 11. The immobilized enzyme as claimed in claim 9, wherein
- the specific volume activity is in the range of 100 to 250 U per g wet carrier.
- 12. The immobilized enzyme as claimed in claim 1, wherein the enzyme is D-amino acid oxidase and
- the weight ratio of enzyme to carrier material is in the range of 5 to 25 mg protein per g wet carrier.
- 13. The immobilized enzyme as claimed in claim 12, wherein
- the weight ratio of enzyme to carrier material is in the range of 8 to 15 mg protein per g wet carrier.
- 14. The immobilized enzyme as claimed in claim 12, wherein
- the specific volume activity is in the range of 100 to 200 U per g wet carrier.
- 15. The immobilized enzyme claimed in claim 12, wherein
- the enzyme is co-immobilized with catalase.
- 16. A method of synthesizing a modified penicillin or cephalosporin antibiotic comprising synthesizing the antibiotic in a reaction catalyzed by an immobilized enzyme as claimed in claim 1.
- 17. The method as claimed in claim 16, wherein
- the reaction is carried out at a temperature of to 30.degree..
- 18. The method as claimed in claim 16, wherein
- the reaction takes place in the presence of an organic solvent.
- 19. A process of preparing an immobilized enzyme comprising immobilizing an enzyme selected from the group consisting of penicillin-G amidase, glutaryl-7-ACA acylase and D-amino acid oxidase, comprising
- on an aminofunctional organosiloxane polymer carrier material having an average particle diameter of 0.01 to 3 mm by covalent binding to provide an immobilized enzyme having a specific volume activity of at least 100 U/g wet carrier material.
- 20. The process as claimed in claim 19, wherein
- immobilizating of the enzyme on the carrier material is achieved by means of a dialdehyde.
- 21. The process as claimed in claim 19, wherein
- the weight ratio of enzyme to carrier material is in the range of 1 to 300 mg protein per g wet carrier.
- 22. The process as claimed in claim 19, wherein
- a carrier material with an average particle size of 0.1 to 0.4 mm is used.
- 23. The process as claimed in claim 19, wherein
- a spherical carrier material is used.
Priority Claims (1)
Number |
Date |
Country |
Kind |
43 42 770.7 |
Dec 1993 |
DEX |
|
Parent Case Info
The application is a 371 of PCT/EP94/04132, filed Dec. 13, 1994.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/EP94/04132 |
12/13/1996 |
|
|
5/30/1996 |
5/30/1996 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO95/16773 |
6/22/1995 |
|
|
Foreign Referenced Citations (2)
Number |
Date |
Country |
231093 |
Aug 1987 |
EPX |
496993 |
Aug 1992 |
EPX |
Non-Patent Literature Citations (2)
Entry |
Danzig, et. al., Indian Journal of Chemistry, vol. 32 B, Jan. 1993, pp. 40-43. |
Brink, et. al., Enzyme Microb. Technol., vol. 10, Dec. 1988, pp. 736-743. |