Abstract The overall goal of this project is to develop an optimized assay design and development process to permit the rapid implementation of sensitive, bead-based immuno-PCR assays for the detection of the major bacterial and fungal toxins that are produced by common contaminants of medicinal cannabis. The assay process will be developed and validated in Phase I, focusing on one important small molecule mycotoxin, paxilline. Our phase II plans for this project will be to utilize the optimized assay format to develop and commercialize sensitive bead-based iPCR assays to detect each of the major bacterial and fungal toxins that are produced by prevalent contaminants of medicinal cannabis. This will address a critical unmet need in the rapidly expanding medicinal cannabis industry and will enhance MGC's list of product offerings significantly.