Patent Application
20060177882
The present invention is related to immunoassays with enhanced selectivity for detecting the presence or absence of a target analyte which uses a molecularly imprinted polymer (MIP) having low affinity to the target analyte, but high affinity for one or more cross-reactive substances.
Affinity-based immunoassays, due to their sensitivity, are routinely used to detect and measure the presence and the concentration of an analyte in a sample. The analyte may be any of the wide variety of materials, such as drugs, pollutants, chemicals, contaminants, or the like. These assays need high-affinity specific receptors, but obtaining a very specific receptor that will only bind to one specific analyte is not an easy task because there are generally several analogues and/or metabolites of the target analyte, which we will call cross-reactive substances.
An established method to decrease the deteriorating effects of cross-reactive substances is to employ a two-site binding assay. In this case, two receptors are used which selectively recognize two independent sites of a target molecule. Unfortunately, this method is not applicable when the analyte has a low molecular mass (<1000). In these cases, competitive immunoassays represent the most common approach for performing detection and quantification. These immunoassays, whatever their format, contain a target analyte, a specific antibody that binds to the analyte and a corresponding labeled-analyte, which consists of a derivative of the target molecule attached to a detectable label such as an enzyme. The principal strategy for improving the assay selectivity has been oriented at improving the specificity of the detecting antibody by judicious choice of hapten used to raise antibodies. Another strategy, suggested in U.S. Pat. No. 6,306,616, is based on neutralizing the effect of the interfering substance in the sample. This technique, in principle is used to confirm the positive assay, and thus is not intended for quantitative measurement of the analyte.
An interesting possibility as a sorbent medium for the removal of cross-reactants from an assay involves the use of molecularly imprinted polymers (MIPs). The preparation of an MIP is based on the principle of utilizing the functionality of a target molecule (the template), in order to assemble its own recognition site by forming interactions with complementary functional groups of appropriate functional monomers. These interactions are then frozen by polymerization, carried out in a solution with a high concentration of cross-linker. Subsequent removal of the template by extraction creates binding sites, ideally maintaining the precise spatial arrangement of the functional groups. Thus, reversible re-binding and highly selective recognition of the target molecule is achievable, and it is possible to perform MIP-based assays.
Such materials, owing to their specificity, short development time, ease of preparation, low price and high chemical and physical stability, have also been considered as a viable alternative to antibodies for use as receptors in immunoassays. In general, however, the reduced affinity relative, inhomogeneity of binding sites, and lack of reproducibility make MIPs better suited to applications involving separation and extraction of substances rather than complete assays. Recently, it has been shown by Spegel (P. Spegel et al., Anal Chem 75 (2003) 6608-6613) that it is possible to template an MIP for multiple analytes. Although such a multiple-templated MIP was discussed in the limited context of a recognition element for the separation and detection of multiple target analytes, it is also possible to use it as a means of absorbing multiple cross-reactants in order to obtain an assay with enhanced specificity for a single analyte, as described in the present invention.
A novel usage of MIPs as sorbent media for the removal of cross reactants has been described in U.S. Pat. No. 6,461,873, in which a device using an MIP to enhance the selectivity of caffeine detection is disclosed. The device is a chromatographic caffeine detector that uses the finite absorption of a MIP templated for caffeine as a chromatographic medium, where samples with higher concentrations of caffeine migrate further up the chromatographic medium. Chromogenic reagents are used to enable a colourimetric measurement of the caffeine migration distance, which can be directly related to the caffeine concentration. The device also includes a sorbent zone, in which the sample is passed through a strip of MIP that absorbs cross-reactive substances that interfere with the quantification of caffeine. This usage of the MIP sorbent zone increases the specificity and detection limit of the subsequent chromatographic assay. Although this device appears to perform well as a detector for caffeine, it is limited in the scope of its physical nature and assay format to strip-based chromatographic assays.
What is therefore needed is a method of performing an MIP-based sorbent assay for non-chromatographic binding assays, especially those in which multiple cross-reactants are present.
The disadvantages of immunoassays resulting from the cross-reactivity of receptors are overcome by using the methods of this invention. In the preferred embodiment, a sample is assayed in two steps. In the first step, a liquid sample is brought in contact with a molecularly imprinted polymer (MIP) having low affinity to the target analyte, but high affinity for one or more cross-reactive substances. Thus, the concentrations of the cross-reacting species in the liquid sample are reduced. In the second step, the liquid sample is separated from the MIP and assayed with a binding assay known in the prior art to determine the presence or absence of the target analyte. If the separated liquid sample is aliquoted into several separate vessels, then multiple binding assays for different target analytes can be performed. The MIP employed in the invention can be either cast for a single cross-reactant or cast for multiple cross-reactants that may be present in the sample. A multi-receptor MIP absorbs multiple interfering cross-reactants and dramatically increases the specificity of the subsequent assay.
Thus, in one aspect the present invention provides a method of performing a binding assay for one or more target analytes, comprising the steps of:
According to another aspect of the invention, an MIP cast for one or more cross-reactants is incorporated into a lateral flow assay binding assay device. The device includes a section of a porous strip that incorporates an MIP cast for one or more cross-reactants. As the sample and reagents flow through the layer of the porous strip containing the MIP, cross-reactants are absorbed and the specificity of the assay is enhanced. The subsequent binding of analyte and reagents in the capture zone of the lateral flow porous strip produces a result that has an increased specificity over a prior-art device lacking the MIP section.
Thus, in another aspect of the invention there is provided a lateral flow binding assay device comprising:
A further understanding of the functional and advantageous aspects of the invention can be realized by reference to the following detailed description and drawings.
The invention will be more fully understood from the following detailed description thereof taken in connection with the accompanying drawings, which form a part of this application, and in which:
The assay method of the present invention employs a molecularly imprinted polymer (MIP) to selectively absorb cross-reactants that would otherwise reduce the specificity of an assay for a target analyte. More specifically, a novel method of conducting an assay is disclosed wherein a sample that may contain a target analyte and also cross-reactants that may interfere with the signal measured to infer the concentration of the target analyte is first contacted with an MIP solid phase that selectively absorbs the cross-reactants. After the sorbent step of the inventive assay, a binding assay known in the prior art may be used to detect the concentration of target analyte in the sample. The removal of cross-reactants via the MIP dramatically increases the specificity of the overall assay.
The term “molecularly imprinted polymer (MIP)”, as used herein, means an artificial receptor for an analyte made by forming recognition sites within a polymer matrix that are adapted to the three-dimensional structure of the analyte molecule, that is, it is templated to absorb one or more of pre-selected analytes (or cross-reactants as in the case of the present invention). MIPs can be engineered via covalent and non-covalent interactions and metal ion coordination. Their synthesis generally involves the interaction of a functional monomer with the analyte, following which the monomer-analyte complex is polymerized via a cross-linker, producing a macroporous polymer with the analyte molecules in a sterically fixed arrangement. Artificial recognition sites for the analyte are obtained after the removal of the analyte from the MIP. Although the traditional recipe for the syntesis of MIPs involves the use of methyl methacrylate (MMA) as a functional monomer and ethylene glycol methacrylate (EGDMA) as the cross-linker, a wide variety of methods and formats are known in the prior art may be used which fall within the scope of the present invention.
The term “receptor”, as used herein, means antibodies, DNA, RNA, aptamers, molecularly imprinted polymers, or any other species capable of exhibiting a specific binding affinity for the analyte.
The phrase “analyte” or “target analyte”, as used herein, means any species whose presence or concentration in a sample is sought.
The phrase “cross-reactant”, as used herein, means any species whose presence or concentration in a sample produces a signal that can be misinterpreted as having been generated by the presence of analyte.
The phrase “binding assay”, as used herein, means an assay in which the affinity of a receptor for a target analyte is employed to bind the receptor to the analyte a produce a measurable signal that is proportional to the analyte concentration.
In one embodiment of the invention, an immunoassay is employed as schematically illustrated in
An example of such a binding assay is a homogeneous, non-separation enzyme based competitive assay. Another example is a two-site binding enzyme-linked immunosorbent assay (ELISA). Since the first step does not alter the composition of the specimen beyond the removal of cross-reactants, the treated specimen can be used in virtually any assay platform for the analyte.
In order to have the maximum possible surface area, it is preferable to prepare MIPs as monosized spherical particles rather than as monoliths that must be fragmented into irregular particles. An example of a prior-art method to achieve this is U.S. Pat. No. 5,872,198, in which suspension polymerization in perfluorocarbon liquids was employed, which patent is incorporated herein in its entirety. Alternatively, magnetic beads coated with an MIP can be used in the sorbent vessel for the initial absorption step. Such beads can be prepared by techniques provided in the prior art, e.g., U.S. Pat. No. 6,316,235, which patent is incorporated herein in its entirety. The separation of the treated liquid sample from the MIP solid phase can then be achieved using a magnetic field.
Without being bound by any theory, the preceding description of the inventive assay method is henceforth described via a specific non-limiting mathematical example. The immunoassay involves one type of labeled-analyte (Ag*), with initial concentration of Cc0, is competing with analyte in the solution (Ag), with initial concentration of Ca0, and a single type of cross-reactive substance (Agr), with initial concentration of Cr0, to bind to antibodies (Ab) coated with an average surface density Σ on a solid surface with total surface area S. The three reactions occurring in the liquid and solid phases are described with the following equilibrium equations (see reference [2])
Ca·σ=Kaσa, (1)
Cr·σ=Krσr, (2)
Cc·σ=Kcσc, (3)
where σ is the surface density of un-reacted Ab, σa, σr and σc are the surface densities of AgAb, Ag*Ab and AgcAb, and Ca, Cc and Cr are the equilibrium concentrations of Ag, Ag* and Agr, respectively. The law of the conservation of reagents results in
Ca+fσa=Ca0, (5)
Cr+fσr=Cr0, (6)
Cc+fσc=Cc0, (7)
σ+σa+σr+σc=Σ, (8)
where f≡S/V, and V is the reaction volume of the immunoassay. In
Now consider a case that the sample is pretreated by incubating it with a MIP surface, with surface area S1, coated with receptors, R, with density of Σ1. The volume of the incubation well is V1. The two reactions occurring in the liquid and solid phases are described with the following equilibrium equations
Ca1·σ1=Ka1σa1, (9)
Cr1·σ1=Kr1σr1, (10)
where σ1 is the surface density of un-reacted receptors, σa1 and σr1 are the surface density of AgR, and AgrR, Ca and Cr (mol/l) are the equilibrium concentrations of Ag and Agr, respectively. The law of the conservation of reagents results in
Ca1+f1σa1=Ca0, (11)
Cr1+fσr1=Cr0, (12)
σ+σa1+σr1=Σ1. (13)
Solving these equations demonstrates that as a result of the pretreatment, the concentration of both analyte and the cross-reactive substance decrease, although with different magnitudes. For the values Ka1=20 nM, Kr1=1 nM, f1=5/0.2×10−4=2.5×105 cm2/L and Σ=3.0×10−3 nM/cm2, we have presented the value of Ca1/Cr1 as a function of Ca0 in
Although the above method has illustrated the use of an MIP templated for a single cross-reactant, a preferred embodiment of the invention uses multiple MIPs to selectively absorb multiple cross-reactants. One method of producing an MIP that can absorb multiple cross-reactants is to mix several MIPs that are separately made for single cross-reactants. Prior-art methods for making MIPs with multiple solid phases, including MIP nanoparticles and MIP-coated magnetic beads, are preferably used for producing singly-templated MIPs that can be combined to form the desired mixed MIP.
Another approach for producing an MIP that can absorb multiple cross-reactants is to create an MIP that is templated with multiple cross-reactants in a single step. Such MIPs, henceforth referred to as multi-receptor MIPs have been studied in the academic literature and methods for their synthesis are known (P. Spegel et al., Anal Chem 75 (2003)6608-6613). The formation of a suitable solid surface for use as an absorber can be achieved using any of several prior-art methods, including the formation of MIP nanoparticles and the synthesis of MIP magnetic beads. In another preferred embodiment of the invention, the multi-receptor MIP is coated onto the surface of a vessel, for example the bottom of a microplate well or the inner surface of a pipette tip.
The preceding discussion clearly illustrates the dramatic performance enhancement obtained by pre-treating the sample with a MIP that absorbs one or more potential cross-reactants. The removal of cross-reactants in a systematic and controlled manner surmounts a key limitation in binding assay technology: non-specificity. This drawback has limited the use of binding assays, in particular immunoassays, to the realm of qualitative or semi-quantitative assays due to their propensity for generating false-positive results. Indeed, immunoassays are often simply used to screen out negative results, in which case any positive results are sent for expensive and time-consuming confirmatory testing. With the removal of multiple known cross-reactants from the sample prior to conducting the assay, the multiple MIP approach enables confirmatory-type immunoassays in a simple and inexpensive platform.
In addition to enabling confirmatory-type immunoassays, the invention also provides a means of using sub-optimal antibodies for screening assays. In many cases, it is not feasible to generate antibodies with high affinity and/or specificity for a given analyte. This may be due to a multitude of factors that result in inferior antibodies, including difficulties in generating suitable derivatives of small molecule analytes in order to generate in immunogenic response and constraints on development time. The multiple MIP scheme disclosed herein allows the use of such inferior antibodies, since the pretreatment step in which the multiple MIP absorbs cross-reactants effectively multiplies the specificity of the subsequent assay by a potentially large factor.
In another embodiment, the sample is introduced into a column that is packed with a multiple MIP. The sample flows through the column (pressure may be applied via a pumping mechanism) and the MIP absorbs the cross-reactants owing to the very high surface area inside the column. After the sample flows through the column, it is collected into an intermediate vessel, from where it is accurately pipetted into a reaction vessel. In this scheme, the sorbent column can either be made disposable or eluted and washed for reuse.
Although the assay is preferably carried out by first contacting the sample with the MIP and subsequently performing the assay with a purified form of the sample, the assay may also be conducted simultaneously with the MIP in a non-separation format. However, this scheme is usually precluded by the need to have a high MIP surface area to liquid volume ratio. If this ratio is not sufficiently high, only a modest fraction of the cross-reactants will be absorbed by the MIP and the assay specificity will not be dramatically enhanced.
An MIP can also be used as a sorbent material in a lateral flow binding assay in order to increase the assay specificity. Referring to
As the sample and solubilized reagents percolate through this MIP zone 16 via capillary action, cross-reactants are absorbed. After passing through the MIP zone 16, the sample and solubilized reagents encounter the capture zone 18. The immobilization of analyte or reagents within the capture zone produces a visual measure of the analyte concentration. A control line may be integrated in the capture zone to enhance the accuracy of the assay result. An absorption pad 20 located beyond the capture zone absorbs sample and reagents as they pass through the capture zone, eliminating backflow. The absorption of interfering cross-reactants by the MIP dramatically decreases the incidence of false-positive assay results.
In another embodiment of the invention, the sample first passes through the MIP zone 16 before encountering an additional zone containing one or more dried reagents, where this additional zone is located between the MIP zone 16 and the capture zone 18. In this format, the MIP advantageously absorbs cross-reactants that may be present before the sample solubilizes and possibly reacts with the dried reagents. The pre-absorption of cross-reactants thus reduces or eliminates the potential of cross-reactants to react with the reagents and produce an erroneous assay result.
It will be apparent to those skilled in the art that there are many possible forms of the invention that, while differing from the aforementioned embodiments, do not depart from the scope and theme of the invention. For example, the binding agent in the final assay may also be a MIP, or alternatively an aptamer, instead of an antibody.
As used herein, the terms “comprises”, “comprising”, “including” and “includes” are to be construed as being inclusive and open ended, and not exclusive. Specifically, when used in this specification including claims, the terms “comprises”, “comprising”, “including” and “includes” and variations thereof mean the specified features, steps or components are included. These terms are not to be interpreted to exclude the presence of other features, steps or components.
The foregoing description of the preferred embodiments of the invention has been presented to illustrate the principles of the invention and not to limit the invention to the particular embodiment illustrated. It is intended that the scope of the invention be defined by all of the embodiments encompassed within the following claims and their equivalents.
