Patent Application
20190117756
A computer readable form of the Sequence Listing “21806-P45290US03_SequenceListing.txt” (798,720 bytes), submitted via EFS-WEB and created on Oct. 23, 2018, is herein incorporated by reference.
The present disclosure relates to immunogenic compositions and vaccines and methods of making and evaluating thereof. More in particular, the disclosure relates to immunogenic compositions derived from bacterial surface receptor proteins and to vaccines against Gram-negative bacterial organisms, including, but not limited to, bacterial organisms belonging to the bacterial families of Pasteurellaceae, Neisseriaceae and Moraxellaceae.
The following paragraphs are intended to introduce the reader to the more detailed description that follows and not to define or limit the claimed subject matter of the present disclosure.
Vaccines capable of mediating an effective immune response are important in health strategies aimed at combating diseases caused by microbial pathogens. The two basic strategies for inducing an effect immune response in the host involve either the administration to a subject (host) of a ‘live’ agent capable of replicating within the host, or the administration of materials or substances that are not capable of replicating in the host. Administration of a live vaccine may represent a safety risk for immune-compromised individuals if the agent or a contaminating organism replicate and adversely affect the immunized subject. These risks are not associated with vaccines based on killed whole pathogens, based on extracts from pathogens or based on purified components, commonly referred to as subunit vaccines. Subunit vaccines avoid the safety problems associated with live vaccines but the purified components may not by themselves deliver the desired protective effect in the subject against the infective microbial organism and require appropriate components, termed adjuvants, to enhance the immune response.
Approaches to the design of vaccines against Gram-negative bacterial organisms have commonly focused on the use of proteins that are naturally associated with the outer membrane of the bacteria and exposed on the surface of the bacterial cells. Particularly attractive targets for vaccination are proteins presumed to be critical for survival in the host as they cannot be lost or dramatically altered in order to avoid the host immune response. In this respect the bacterial surface receptor proteins capable of interacting with and binding to the host iron binding proteins, transferrin and lactoferrin, have for some time been considered suitable components for use in the preparation of vaccines (1-3). This group of surface receptor proteins, hereinafter referred to as “HIBP” (host iron binding protein) surface receptor proteins, is present in pathogens of humans and animals belonging to the bacterial families Pasteurellaceae, Moraxellaceae and Neisseriaceae (4). Thus these proteins have been recognized as potential targets for development of vaccines against a variety of different pathogens of humans and food production animals (5) (6-10).
The HIBP surface receptors normally are comprised of two proteins, a surface lipoprotein, transferrin binding B (TbpB) or lactoferrin binding protein B (LbpB), and a TonB-dependent, integral membrane protein, transferrin binding protein A (TbpA) or lactoferrin binding protein A (LbpA) (11). Recently the detailed three-dimensional structures of TbpBs from Actinobacillus pleuropneumoniae, Actinobacillus suis, and Neisseria meningitidis were determined at high resolution (12-14). The intrinsic properties of the TbpB or LbpB proteins are quite different from the integral outer membrane proteins, TbpA and LbpA, and substantially impact on the strategies used for vaccine development. For instance, it is possible to produce and purify TbpB or LbpB at relatively high yields from the E. coli cytoplasm for the generation of subunit vaccines. However, these proteins are notably absent or deficient in outer membrane vesicle (OMV) vaccines prepared by selective detergent extraction due to their removal during the extraction process. In contrast, functional TbpA or LbpA can only be produced in the outer membrane, providing limitations for producing high yields of purified proteins to be used in subunit vaccines. The alternate approach of producing misfolded proteins that aggregate into large inclusion bodies and subsequently attempt to refold the protein from the enriched inclusion body preparations, are also problematic for commercial production. Thus most strategies for TbpA or LbpA based vaccines would normally involve production of OMVs or development of attenuated strains.
An alternate approach that has been used successfully for invasive bacterial pathogens is to use the extracellular capsular polysaccharide as the primary antigen, and couple it to a protein carrier to induce T-cell help. These conjugate capsular vaccines have proven to be very effective at providing protection from infection by strains expressing the specific capsular polysaccharide but provide no cross-protection to other capsular types. Although conjugate capsular vaccines against the human pathogens H. influenzae, N. meningitidis and Streptococcus pneumoniae were originally developed to prevent invasive infection, post licensure carriage studies have demonstrated that the systemically administered vaccines eliminated colonization by the pathogens expressing the specific targeted polysaccharides (15-17). This had the added benefit of providing herd immunity, providing protection to non-immunized individuals due to reduced carriage frequency within the population. While not the initial intent of these early vaccines, the potential to confer herd immunity has become an important criterium for evaluating new and upcoming bacterial vaccines. However, determining or predicting whether new vaccines will be capable of impacting or preventing colonization (carriage) is a major challenge (18).
The ability to evaluate whether protein antigens will ultimately be capable of providing broad protection against a diverse set of disease isolates is also a considerable challenge (19). Initial efforts at testing this ability commonly involve immunizing other animal species (mice, rabbits) and then analyzing the cross-reactive and cross-protective properties of the resulting sera. For those surface antigens that can readily be produced in a soluble form, such as surface lipoproteins, a first step is often to produce and purify a set of variant proteins and use them in a standard ELISA (enzyme linked immunosorbent assay) to evaluate the ability of the antisera to recognize the variant proteins. This is fairly labor intensive making analysis of an extensive set of variants an expensive enterprise and relies on the assumption that the binding of the antigen to the ELISA plate is random so that all protein surfaces can be probed.
Considering the limitations in the various assays used to evaluate and predict the cross-protective and cross-reactive properties of antisera raised against antigens, the selection and design of new and improved protein-based vaccines should be pursued in conjunction with development of improved assays so that optimizing and improving vaccines in development can be approached on a rational basis.
Despite considerable efforts over the years since their initial discovery (20, 21), it remains unclear whether and how HIBP surface receptor proteins can be used to prepare efficacious vaccines against Gram-negative bacterial pathogens, and in particular whether and how a broadly protective vaccine can be developed. Thus there is a need in the art to improve immunogenic compositions and vaccines based on HIBP surface receptor proteins against Gram-negative bacterial organisms.
The present disclosure provides novel immunogenic compositions and in particular immunogenic compositions based on HIBP surface receptor proteins from Gram-negative pathogenic bacterial species.
Accordingly, the present disclosure provides, in at least one embodiment, an immunogenic composition comprising an antigen derived from a HIBP surface receptor protein from a Gram-negative pathogenic bacterial species, wherein the protein derived from the HIBP surface receptor protein has been modified in such a manner that it is unable to substantially bind host iron binding protein.
The present disclosure provides, in at least one embodiment, an immunogenic composition comprising a polypeptide comprising a C-lobe domain or an N-lobe domain of a HIBP surface receptor protein obtainable from or obtained from a Gram-negative bacterial species, wherein the polypeptide is unable to substantially bind host iron binding protein. In preferred embodiments, the present disclosure provides an immunogenic composition comprising a C-lobe domain of a HIBP surface receptor polypeptide, wherein the polypeptide is unable to substantially bind the host iron binding protein. In further preferred embodiments, the present disclosure provides an immunogenic composition comprising a mixture of at least two polypeptides, each polypeptide comprising a C-lobe domain, wherein the C-lobe domains are obtainable or obtained from at least two Gram-negative bacterial species, or from at least two Gram-negative bacterial strains. In further preferred embodiments, the C-lobe domains are obtained from the HIBP surface receptor proteins that are antigenically divergent.
In further embodiments, the present disclosure further provides an immunogenic composition comprising a polypeptide comprising an N-lobe domain and/or a C-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a Gram-negative pathogenic bacterial species, wherein the N-lobe domain or the C-lobe domain comprise a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains of the N-lobe domain or the C-lobe domain has been modified, and wherein the polypeptide is unable to substantially bind host iron binding protein. In preferred embodiments, the modification comprises the modification of at least one amino acid residue within a loop domain.
In further embodiments, at least two loop domains of the plurality of loop domains within the C-lobe domain and/or the N-lobe domain of an HIBP surface receptor protein have been modified.
In further embodiments, the present disclosure provides (i) a first polypeptide, comprising an N-lobe domain or a C-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a Gram-negative pathogenic bacterial species, wherein the N-lobe domain or the C-lobe domain comprise a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains of the N-lobe domain or the C-lobe domain has been modified, linked to (ii) a second polypeptide comprising an HIBP surface receptor protein, or a portion thereof, obtainable from a Gram-negative bacterial species, and wherein the linked polypeptide is unable to substantially bind host iron binding protein. In preferred embodiments, the portion of the HIBP surface protein is an N-lobe domain or a C-lobe domain. In further preferred embodiments, the portion of the HIBP surface protein is an N-lobe domain or a C-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a Gram-negative pathogenic bacterial species, wherein the N-lobe domain or the C-lobe domain comprise a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains of the N-lobe domain or the C-lobe domain has been modified.
In further embodiments, the C-lobe domain or the N-lobe domain is a C-lobe domain or an N-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a bacterial species belonging to the bacterial family of Pasteurellaceae, Moraxellaceae or Neisseriaceae, and in further preferred embodiments, the C-lobe domain or the N-lobe domain is a C-lobe domain or an N-lobe domain of an HIBP surface receptor polypeptide obtainable from or obtained from a bacterial species belonging to the bacterial genus of Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella.
In further preferred embodiments, the HIBP surface receptor protein is modified in such a manner that the N-terminal anchor polypeptide of the HIB surface receptor protein, or a portion thereof, is removed, and wherein the polypeptide is unable to substantially bind host iron binding protein.
In other aspects, the present disclosure provides methods for preparing an immunogenic composition. Accordingly, the present disclosure provides a method for preparing an immunogenic composition comprising:
In further embodiments, the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, wherein at least one loop domain of the plurality of loop domains has been modified.
In yet further aspects, methods for eliciting an immune response in a vertebrate subject are provided. Accordingly, the present disclosure further provides a method for eliciting an immune response in a vertebrate subject, said method comprising administering to the subject:
In preferred embodiments, the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, wherein at least one loop domain of the plurality of loop domains has been modified.
The present disclosure further includes an immunogen comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor polypeptide wherein the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain has been modified, for use as a medicament.
The present disclosure further includes an immunogen comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor used in the prevention of infection, for example by prevention of colonization, or disease by infectious Gram-negative bacteria, including bacteria belonging to the genus Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella. In preferred embodiments, the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, wherein at least one loop domain of the plurality of loop domains has been modified.
The present disclosure further includes an immunogen comprising a C-lobe domain and/or an N-lobe domain of an HIBP surface receptor polypeptide for use in the manufacture of a medicament for the prevention of infection or disease by infectious Gram-negative bacteria, including bacteria belonging to the genus Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella. In preferred embodiments, the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, wherein at least one loop domain of the plurality of loop domains has been modified.
The immunogenic compositions of the present disclosure may be used to prepare a vaccine. Accordingly, the present disclosure further provides a vaccine composition comprising an antigen derived from a HIBP surface receptor protein from a Gram-negative pathogenic bacterial species, wherein the protein derived from the HIBP surface receptor protein has been modified in such a manner that it is unable to substantially bind host iron binding protein.
In further embodiments, the present disclosure further also includes a vaccine composition comprising a polypeptide comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor protein obtainable from a Gram-negative pathogenic bacterial species wherein the polypeptide is unable to substantially bind host iron binding protein.
In further embodiments, the vaccine composition comprises a polypeptide comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor polypeptide wherein the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain has been modified and wherein the polypeptide is unable to substantially bind host iron binding protein.
The present disclosure further provides methods for administering a vaccine to a vertebrate subject, the method comprising administering to the subject a vaccine comprising a polypeptide comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor protein obtainable from a Gram-negative pathogenic bacterial species, wherein the vaccine is administered in an amount sufficient to prevent or treat a disease caused by a Gram-negative bacterial species.
The present disclosure further includes a vaccine comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor for use in the prevention of infection or disease by infectious Gram-negative bacteria, including bacteria belonging to the genus Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella.
In preferred embodiments, the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and at least one loop domain of the plurality of loop domains has been modified.
Other features and advantages of the present disclosure will become apparent from the following detailed description. It should be understood, however, that the detailed description, while indicating preferred embodiments of the disclosure, are given by way of illustration only, since various changes and modifications within the spirit and scope of the disclosure will become apparent to those of skill in the art from the detailed description.
The disclosure is in the hereinafter provided paragraphs described in relation to its Figures. The Figures provided herein are provided for illustration purposes and are not intended to limit the present disclosure.
TABLES 1-3 represent combinations of 1, 2 or 3 loop domains, respectively, selected from the loop domains L1-L32 of HIBP polypeptides, which may be modified in accordance with the present disclosure.
Various compositions and methods will be described below to provide an example of an embodiment of each claimed subject matter. No embodiment described below limits any claimed subject matter and any claimed subject matter may cover methods, processes, compositions or systems that differ from those described below. The claimed subject matter is not limited to compositions or methods having all of the features of any one composition, method, system or process described below or to features common to multiple or all of the compositions, systems or methods described below. It is possible that a composition, system, method or process described below is not an embodiment of any claimed subject matter. Any subject matter disclosed in a composition, system, method or process described below that is not claimed in this document may be the subject matter of another protective instrument, for example, a continuing patent application, and the applicants, inventors or owners do not intend to abandon, disclaim or dedicate to the public any such subject matter by its disclosure in this document.
It should be noted that terms of degree such as “substantially”, “essentially” “about” and “approximately” as used herein mean a reasonable amount of deviation of the modified term such that the end result is not significantly changed. These terms of degree should be construed as including a deviation of the modified term if this deviation would not negate the meaning of the term it modifies.
As used herein, the wording “and/or” is intended to represent an inclusive-or. That is, “X and/or Y” is intended to mean X or Y or both, for example. As a further example, “X, Y, and/or Z” is intended to mean X or Y or Z or any combination thereof.
All publications, patents and patent applications are herein incorporated by reference in their entirety to the same extent as if each individual publication.
As hereinbefore mentioned, the present disclosure provides novel immunogenic compositions, and in particular immunogenic compositions based on the HIBP surface receptor proteins from Gram-negative pathogenic bacterial species, such as Neisseria meningitidis. The immunogenic compositions of the present disclosure are useful in that they may be employed to prepare novel vaccine formulations to protect humans and animals against infective pathogenic Gram-negative bacterial species. In accordance with the present disclosure, the HIBP surface receptor proteins of the present disclosure are modified in such a manner that they are unable to substantially bind host iron binding proteins. Such modified HIBP surface receptor proteins exhibit unexpectedly strong immunogenic properties. Furthermore the immunogenic compositions of the present disclosure are substantially stable polypeptides and therefore may readily be manufactured. Moreover, and importantly, the immunogenic compositions of the present disclosure are unexpectedly effective, for example by inducing a cross-reactive immune response, thus permitting protection against multiple pathogenic microbial organisms by the administration of a single efficacious vaccinating compound. The vaccines prepared in accordance with the present disclosure do not contain live organisms or crude extracts, thereby representing a very limited health risk.
Accordingly, the present disclosure provides, in at least one embodiment, an immunogenic composition comprising an HIBP surface receptor protein from a Gram-negative pathogenic bacterial species, wherein the HIBP surface receptor protein has been modified in such a manner that it is unable to substantially bind host iron binding protein.
The present disclosure further provides an immunogenic composition comprising a polypeptide comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor protein obtainable from a Gram-negative pathogenic bacterial species, wherein the polypeptide is unable to substantially bind host iron binding protein. In certain embodiments, the N-lobe domain or the C-lobe domain of the HIBP surface receptor protein comprises a plurality of β-strands connected by a plurality of loop domains, and one of more loop domains of the plurality of loop domains has been modified.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosure pertains. All patents and patent applications, and other publications, including nucleic acid and polypeptide sequences from GenBank, SwissPro and other databases, cited herein, whether supra or infra, are hereby incorporated by reference in their entirety, where permitted. It is further noted that, as used in this specification and the appended claims, the singular forms “a”, “an”, and “the” include plural referents unless the content clearly dictates otherwise. Thus, for example, reference to “an immunogen” includes a mixture of two or more such agents, reference to “a polypeptide” includes reference to mixtures of two or more polypeptides, reference to “a cell” includes two or more such cells, and the like.
The terms “immunogen” and “immunogenic composition”, as interchangeably used herein, are used in their broadest sense to refer to a molecule which contains one or more epitopes that will stimulate the immune response in a host organism to generate a cellular immunogen-specific immune response, and/or a humoral antibody response. Immunogens include nucleic acids, proteins, polypeptides, peptides and immunogenic protein fragments.
The terms “vaccine” and “vaccine composition”, as interchangeably used herein, refer to any pharmaceutical composition containing an immunogen, which composition can be used to prevent or treat a disease or condition in a subject. The terms thus encompass subunit vaccines, i.e., vaccine compositions containing immunogens which are separate and discrete from a whole organism with which the immunogen is associated in nature.
The term “vertebrate subject” refers to any member of the subphylum cordata, particularly mammals, including, without limitation, humans and other primates. The term does not denote a particular age. Thus both newborn, infant, child and adult individuals are intended to be covered.
The interchangeably herein used terms “HIBP surface receptor protein”, “HIBP surface receptor polypeptide”, “host iron binding protein surface receptor protein” or “host iron binding protein surface polypeptide” refer to any membrane anchored protein or polypeptide obtainable from a Gram-negative bacterial species capable of interacting with host iron-binding proteins. The term includes, any TbpB and LbpB proteins. HIBP surface receptor proteins when folded in their native three-dimensional structure, are comprised of a bi-lobal structure comprising an N-lobe domain and a C-lobe domain, the N-lobe domain and the C-lobe domain each comprising a plurality of β-strands assembled in a β-barrel, and a plurality of β-strands assembled in a β-sheet structure adjacent to the β-barrel, referred to as the handle domain, wherein the β-strands are connected by a plurality of loop domains (as further illustrated in
The interchangeably herein used terms “integral outer membrane protein” and “IOM protein” refer to any integral outer membrane protein from Gram-negative bacterial species, including any proteins belonging to the TonB dependent subclass of proteins, which when folded in their native 3 dimensional structure comprise a 22-stranded C-terminal beta-barrel domain and N-terminal plug or cork domain capable of filling a channel in the C-terminal beta-barrel domain. The term includes without limitation and TbpA and LbpA protein. The terms further refer to any and all IOM polypeptide sequences including all including those set forth in SEQ.ID NO: 152; and SEQ.ID NO: 162 and those comprising a sequence of amino acid residues which (i) are substantially identical to the amino acid sequences constituting any IOM protein set forth herein; (ii) are encoded by a nucleic acid sequence capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any IOM protein set forth herein or capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any IOM protein set forth herein, but for the use of synonymous codons. The terms further include any IOM protein precursor polypeptide; or (iii) will use 3V8X or their subdomains as templates when submitted to a structural modeling server such as Phyre2 (http://www.sbg.bio.ic.ac.uk/phyre2/) or Swiss-Model (http://swissmodel.expasy.org/), in the latter selecting automated mode.
The term “N-lobe domain” as used herein refers to the N-terminal portion of a HIBP surface receptor protein comprising a plurality of β-strands connected by a plurality of loop domains, wherein some of the β-strands are configured to form a β-barrel and an adjacent β-sheet structure, termed a handle domain (see:
The term “C-lobe domain” as used herein refers to the C-terminal portion of a HIBP surface receptor protein comprising a plurality of β-strands connected by a plurality of loop domains, wherein some of the β-strands are configured to form a β-barrel and an adjacent β-sheet structure termed a handle domain. Referring further to
The term “loop domain” refers to the polypeptide sequences in a HIBP surface receptor protein that connect two β-strands. These polypeptide sequences may vary considerably in length from several amino acid residues to 150 or more amino acid residues.
The terms “TbpB”, “TbpB protein”, “TbpB polypeptide”, as interchangeably used herein, refer to any and all transferrin binding protein B sequences, including all bacterial TbpB polypeptides and polypeptides comprising a sequence of amino acid residues which is (i) substantially identical to the amino acid sequences constituting any TbpB polypeptides set forth herein, including, without limitation, SEQ.ID NO: 2; SEQ.ID NO: 12; SEQ.ID NO: 28; SEQ.ID NO: 107 to SEQ.ID NO: 115; SEQ ID NO: 117; SEQ.ID NO: 123; SEQ.ID NO: 131 to SEQ.ID NO: 147; SEQ.ID NO: 177; SEQ.ID NO: 178; SEQ.ID NO: 196 to SEQ.ID NO: 204; SEQ.ID NO: 206 to SEQ.ID NO: 212; and SEQ.ID NO: 219 to SEQ.ID NO: 228, or (ii) encoded by a nucleic acid sequence capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any TbpB polypeptide set forth herein or capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any TbpB polypeptide set forth herein, but for the use of synonymous codons. The terms further include mature TbpB polypeptides as as well as any TbpB precursor, including any pre-TbpB, or TbpB comprising an N-terminal or other signal sequence.
The terms “LbpB”, “LbpB protein”, “LbpB polypeptide”, as interchangeably used herein, refer to any and all lactoferrin binding protein B sequences including all bacterial LbpB polypeptides and a polypeptides comprising a sequence of amino acid residues which is (i) substantially identical to the amino acid sequences constituting any LbpB polypeptides set forth herein, including, without limitation, SEQ.ID NO: 285 or (ii) encoded by a nucleic acid sequence capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any LbpB polypeptide set forth herein or capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any LbpB polypeptide set forth herein, but for the use of synonymous codons. The terms further include any LbpB precursor, including pre-LbpB.
The terms “TbpA”, “TbpA protein”, “TbpA polypeptide”, as interchangeably used herein, refer to any and all transferrin binding protein A sequences including all bacterial TbpA polypeptides and polypeptides comprising a sequence of amino acid residues which is (i) substantially identical to the amino acid sequences constituting any TbpA polypeptides set forth herein, including, without limitation, SEQ.ID NO: 152 or (ii) encoded by a nucleic acid sequence capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any TbpA polypeptide set forth herein or capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any TbpA polypeptide set forth herein, but for the use of synonymous codons. The terms further include any TbpA precursor, including pre-TbpA.
The terms “LbpA”, “LbpA protein”, “LbpA polypeptide”, as interchangeably used herein, refer to any and all lactoferrin binding protein A sequences including all bacterial LbpA polypeptides and a polypeptides comprising a sequence of amino acid residues which is (i) substantially identical to the amino acid sequences constituting any LbpA polypeptides set forth herein, including, without limitation, SEQ.ID NO: 162, or (ii) encoded by a nucleic acid sequence capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any LbpA polypeptide set forth herein or capable of hybridizing under at least moderately stringent conditions to any nucleic acid sequence encoding any LbpA polypeptide set forth herein, but for the use of synonymous codons. The terms further include any LbpA precursor, including pre-LbpA.
The term “nucleic acid sequence” as used herein refers to a sequence of nucleoside or nucleotide monomers consisting of naturally occurring bases, sugars and intersugar (backbone) linkages. The term also includes modified or substituted sequences comprising non-naturally occurring monomers or portions thereof. The nucleic acid sequences of the present disclosure may be deoxyribonucleic acid sequences (DNA) or ribonucleic acid sequences (RNA) and may include naturally occurring bases including adenine, guanine, cytosine, thymidine and uracil. The sequences may also contain modified bases. Examples of such modified bases include aza and deaza adenine, guanine, cytosine, thymidine and uracil, and xanthine and hypoxanthine.
The herein interchangeably used terms “nucleic acid sequence encoding a HIBP surface receptor protein” and “nucleic acid sequence encoding a HIBP surface receptor polypeptide”, refer to any and all nucleic acid sequences encoding a HIBP surface receptor protein, including any HIBP surface receptor protein and any nucleic acid sequences that encode HIBP surface receptor protein precursors, including, without limitation, those set forth in SEQ.ID NO: 1; SEQ.ID NO: 11; SEQ.ID NO: 27; SEQ.ID NO: 116; SEQ.ID NO: 122; and SEQ.ID NO: 173. As used herein “HIBP surface receptor protein precursor” refers to a HIBP surface receptor protein molecule additionally comprising an N-terminal signal sequence which facilitates export of the polypeptide chain across the cytoplasmic membrane. Nucleic acid sequences encoding a HIBP surface receptor protein further include any and all nucleic acid sequences which (i) encode polypeptides that are substantially identical to the HIBP surface receptor proteins sequences set forth herein; or (ii) hybridize to any HIBP surface receptor protein nucleic acid sequences set forth herein under at least moderately stringent hybridization conditions or which would hybridize thereto under at least moderately stringent conditions but for the use of synonymous codons.
The herein interchangeably used terms “nucleic acid sequence encoding an IOM protein” and “nucleic acid sequence encoding an IOM polypeptide”, refer to any and all nucleic acid sequences encoding an IOM protein, including any IOM protein and any nucleic acid sequences that encode IOM protein precursors, including, without limitation, those set forth in SEQ.ID NO: 151 and SEQ.ID NO: 161. As used herein “IOM protein precursor” refers to an IOM protein molecule additionally comprising an N-terminal signal sequence which facilitates export of the polypeptide chain across the cytoplasmic membrane. Nucleic acid sequences encoding a IOM protein further include any and all nucleic acid sequences which (i) encode polypeptides that are substantially identical to the IOM proteins sequences set forth herein; or (ii) hybridize to any IOM protein nucleic acid sequences set forth herein under at least moderately stringent hybridization conditions or which would hybridize thereto under at least moderately stringent conditions but for the use of synonymous codons.
The herein interchangeably used terms “nucleic acid sequence encoding TbpB”; “nucleic acid sequence encoding a TbpB polypeptide”, refer to any and all nucleic acid sequences encoding a TbpB polypeptide, including any TbpB polypeptide, including, without limitation, the sequences set forth in SEQ.ID NO: 1; SEQ.ID NO: 11; SEQ.ID NO: 27; SEQ.ID NO: 116; SEQ.ID NO: 122; and SEQ.ID NO: 173, and further including any nucleic acid sequences that encode TbpB precursors. As used herein “TbpB precursor” refers to a TbpB molecule additionally comprising an N-terminal signal sequence which facilitates export of the polypeptide chain across the cytoplasmic membrane. Nucleic acid sequences encoding a TbpB polypeptide further include any and all nucleic acid sequences which (i) encode polypeptides that are substantially identical to the TbpB polypeptide sequences set forth herein; or (ii) hybridize to any TbpB nucleic acid sequences set forth herein under at least moderately stringent hybridization conditions or which would hybridize thereto under at least moderately stringent conditions but for the use of synonymous codons.
The herein interchangeably used terms “nucleic acid sequence encoding LbpB”; “nucleic acid sequence encoding a LbpB polypeptide”, refer to any and all nucleic acid sequences encoding a LbpB polypeptide, including any LbpB polypeptide, including, without limitation the sequence set forth in SEQ.ID NO: 284 and any nucleic acid sequences that encode LbpB precursors. As used herein “LbpB precursor” refers to a LbpB molecule additionally comprising an N-terminal signal sequence which facilitates export of the polypeptide chain across the cytoplasmic membrane. Nucleic acid sequences encoding a LbpB polypeptide further include any and all nucleic acid sequences which (i) encode polypeptides that are substantially identical to the LbpB polypeptide sequences set forth herein; or (ii) hybridize to any LbpB nucleic acid sequences set forth herein under at least moderately stringent hybridization conditions or which would hybridize thereto under at least moderately stringent conditions but for the use of synonymous codons.
The herein interchangeably used terms “nucleic acid sequence encoding TbpA”; “nucleic acid sequence encoding a TbpA polypeptide”, refer to any and all nucleic acid sequences encoding a TbpA polypeptide, including any TbpA polypeptide, including, without limitation, the nucleic acid sequence set forth in SEQ.ID NO: 151, and any nucleic acid sequences that encode TbpA precursors. As used herein “TbpA precursor” refers to a TbpA molecule additionally comprising an N-terminal signal sequence which facilitates export of the polypeptide chain across the cytoplasmic membrane. Nucleic acid sequences encoding a TbpA polypeptide further include any and all nucleic acid sequences which (i) encode polypeptides that are substantially identical to the TbpA polypeptide sequences set forth herein; or (ii) hybridize to any TbpA nucleic acid sequences set forth herein under at least moderately stringent hybridization conditions or which would hybridize thereto under at least moderately stringent conditions but for the use of synonymous codons.
The herein interchangeably used terms “nucleic acid sequence encoding LbpA”; “nucleic acid sequence encoding a LbpA polypeptide”, refer to any and all nucleic acid sequences encoding a LbpA polypeptide, including any
LbpA polypeptide, including, without limitation, the nucleic acid sequence set forth in SEQ.ID NO: 161 and any nucleic acid sequences that encode LbpA precursors. As used herein “LbpA precursor” refers to a LbpA molecule additionally comprising an N-terminal signal sequence which facilitates export of the polypeptide chain across the cytoplasmic membrane. Nucleic acid sequences encoding a LbpA polypeptide further include any and all nucleic acid sequences which (i) encode polypeptides that are substantially identical to the LbpA polypeptide sequences set forth herein; or (ii) hybridize to any LbpA nucleic acid sequences set forth herein under at least moderately stringent hybridization conditions or which would hybridize thereto under at least moderately stringent conditions but for the use of synonymous codons.
By the term “substantially identical” it is meant that two polypeptide sequences preferably are at least 50% identical, and more preferably are at least 85% identical and most preferably at least 95% identical, for example 96%, 97%, 98% or 99% identical. In order to determine the percentage of identity between two polypeptide sequences the amino acid sequences of such two sequences are aligned, using for example the alignment method of Needleman and Wunsch (26), as revised by Smith and Waterman (27) so that the highest order match is obtained between the two sequences and the number of identical amino acids is determined between the two sequences. A preferred, broadly applicable, method for accurately aligning two polypeptides involves the Clustal W algorithm (28) employed with the BLOSUM 62 scoring matrix (29) using a gap opening penalty of 10 and a gap extension penalty of 0.1. This enables identification of high scoring alignments between two sequences, wherein at least 50% of the total length of one of the two sequences is involved in the alignment. Methods to calculate the percentage identity between two aligned amino acid sequences are generally art recognized and include, for example, those described by Carillo and Lipton (30) and those described in Computational Molecular Biology, Lesk, e.d. Oxford University Press, New York, 1988, Biocomputing: Informatics and Genomics Projects. Generally, computer programs will be employed for such calculations. Computer programs that may be used in this regard include, but are not limited to, GCG (31) BLASTP, BLASTN and FASTA (32).
By “at least moderately stringent hybridization conditions” it is meant that conditions are selected which promote selective hybridization between two complementary nucleic acid molecules in solution. Hybridization may occur to all or a portion of a nucleic acid sequence molecule. The hybridizing portion is typically at least 15 (e.g. 20, 25, 30, 40 or 50) nucleotides in length. Those skilled in the art will recognize that the stability of a nucleic acid duplex, or hybrids, is determined by the Tm, which in sodium containing buffers is a function of the sodium ion concentration and temperature (Tm=81.5° C.-16.6 (Log 10 [Na+])+0.41(% (G+C)−600/l), or similar equation). Accordingly, the parameters in the wash conditions that determine hybrid stability are sodium ion concentration and temperature. In order to identify molecules that are similar, but not identical, to a known nucleic acid molecule a 1% mismatch may be assumed to result in about a 1° C. decrease in Tm, for example if nucleic acid molecules are sought that have a >95% identity, the final wash temperature will be reduced by about 5° C. Based on these considerations those skilled in the art will be able to readily select appropriate hybridization conditions. In preferred embodiments, stringent hybridization conditions are selected. By way of example the following conditions may be employed to achieve stringent hybridization: hybridization at 5× sodium chloride/sodium citrate (SSC)/5×Denhardt's solution/1.0% SDS at Tm (based on the above equation) −5° C., followed by a wash of 0.2×SSC/0.1% SDS at 60° C. Moderately stringent hybridization conditions include a washing step in 3×SSC at 42° C. It is understood however that equivalent stringencies may be achieved using alternative buffers, salts and temperatures. Additional guidance regarding hybridization conditions may be found in: Green and Sambrook, Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory Press, 2012 (33).
The term “chimeric” as used herein in the context of nucleic acid sequences refers to at least two linked nucleic acid sequences which are not naturally linked. Chimeric nucleic acid sequences include linked nucleic acid sequences of different natural origins. For example, a nucleic acid sequence constituting a bacterial promoter linked to a nucleic acid sequence of a TbpB polypeptide or a HIBP surface receptor protein is considered chimeric, and a nucleic acid sequence encoding a TbpB polypeptide from which certain portions have been removed and replaced with portions of a TbpA polypeptide is considered chimeric. Chimeric nucleic acid sequences also may comprise nucleic acid sequences of the same natural origin, provided they are not naturally linked. For example a nucleic acid sequence constituting a promoter obtained from a particular cell-type may be linked to a nucleic acid sequence encoding a polypeptide obtained from that same cell-type, but not normally linked to the nucleic acid sequence constituting the promoter. Chimeric nucleic acid sequences also include nucleic acid sequences comprising any naturally occurring nucleic acid sequence linked to any non-naturally occurring nucleic acid sequence.
By the term “substantially unable to bind host iron binding protein” it is meant that the ability of the host iron binding protein to bind to the HIBP surface receptor protein is diminished in such a manner that the value of the binding constant (Kd) or dissociation constant of the binding interaction between the native host iron binding protein (i.e. the host iron binding protein present in the host organism) and the modified HIBP surface receptor protein is at least 10× higher than the value of the binding constant of the binding interaction between the native host iron binding protein and its complementary native HIBP surface receptor protein. In other words, the modified proteins have a 10× lower affinity for binding native host iron binding protein than the native receptor protein. In preferred embodiments, the relative affinity for binding native host iron binding protein by the modified protein is 30× lower than that of the native HIBP surface receptor protein, and, in most preferred embodiments, the relative affinity for binding native host iron binding protein by the modified protein is 100× lower than that of the native HIBP surface receptor protein. In further preferred embodiments, the binding constant between the modified HIBP surface receptor protein and the native host iron binding protein is at least 300 nM. Preferably the binding constant is at least 500 nM, and most preferably at least 1 μM.
The term “substantially free” as used herein is a term of degree meaning that a composition does not contain significant amounts of a compound of which the composition is said to be substantially free of When a composition is substantially free of a compound, for example substantially free of N-lobe domain, such composition comprises preferably less than 5.0% of such compound, more preferably less than 1.0% of such compound, and most preferably less than 0.1% of such compound.
As hereinbefore mentioned, the present disclosure provides, in at least one embodiment, an immunogenic composition comprising an HIBP surface receptor protein from a Gram-negative pathogenic bacterial species, wherein the HIBP surface receptor protein has been modified in such a manner that it is unable to substantially bind a host iron binding protein. The term “modified” in conjunction with a HIBP surface receptor protein is intended to refer to a non-native HIBP surface receptor protein from which at least one amino acid residue has been removed or in which at least one amino acid residue has been replaced by another, or an HIBP surface receptor protein which has been fragmented in two or more separate polypeptides. Thus modified HIBP surface receptor proteins include, without limitation, truncated HIBP surface receptor proteins (e.g. an N-lobe domain or C-lobe domain of an HIBP surface receptor protein); HIBP surface receptor proteins from which one or more amino acid residues have been removed (e.g. an HIBP surface receptor protein from which one or more amino acids from the loop-domains within the N-lobe domain or C-lobe domain have been removed); HIBP surface receptor proteins in which additional amino acids have been inserted (e.g. HIBP surface receptor proteins in which one or more amino acids have been added to the loop-domains within the N-lobe domain or C-lobe domain); multimeric or extended HIBP polypeptides (e.g. dimers and trimers and N-lobe domain or C-lobe domain dimers and trimers); HIBP surface receptor proteins which have been modified by site-directed mutagenesis to alter one or more amino acids; and mixtures of two or more HIBP surface receptor protein polypeptides (e.g. a mixture comprising a separate N-lobe domains and a C-lobe domains of an HIBP surface receptor protein). The modified HIBP surface receptor proteins of the present disclosure are unable to substantially bind native host iron binding protein.
As hereinbefore mentioned, the present disclosure provides, in one aspect, an immunogenic composition comprising or consisting of a polypeptide comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a Gram-negative bacterial species, wherein the polypeptide is unable to substantially bind host iron binding protein. In further aspects, the present disclosure provides a polypeptide comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor protein wherein the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains has been modified, and wherein the polypeptide is unable to substantially bind host iron binding protein.
In accordance with the present disclosure, any polypeptide or nucleic acid sequence encoding such polypeptide, comprising or consisting of a C-lobe domain or an N-lobe domain of an HIBP surface receptor protein obtainable from a Gram-negative pathogenic bacterial species may be used.
In embodiments of the disclosure where the native C-lobe domain is used, the polypeptide comprising the native C-lobe domain does not comprise and is not chemically linked via a peptide bond to the native N-lobe domain of the HIBP surface receptor protein, and is thus an isolated native C-lobe domain, i.e. a native C-lobe domain separated from the native N-lobe domain. Thus in certain embodiments, preparations comprising a C-lobe domain of an HIBP surface receptor polypeptide free or substantially free of N-lobe domain of an HIBP surface receptor polypeptide, or portions thereof, are provided. In embodiments of the disclosure where the native N-lobe domain is used, the polypeptide comprising the native N-lobe domain used herein does not comprise and is not chemically linked via a peptide bond to the native C-lobe domain of the HIBP surface receptor protein, and is thus an isolated native N-lobe domain, i.e. an N-lobe domain separated from the native C-lobe domain. Thus in certain embodiments, preparations comprising an N-lobe domain of an HIBP surface receptor polypeptide free or substantially free of C-lobe domain of an HIBP surface receptor polypeptide, or portions thereof, are provided. In certain embodiments however, a mixture of a native C-lobe domain, or portions thereof, and the native N-lobe domain, or portions thereof, may be used, provided however, that the N-lobe domain and the C-lobe domain are not chemically linked, i.e. they are not chemically connected by a peptide bond. Thus the present disclosure includes an immunogenic composition comprising a mixture of polypeptides comprising an N-lobe domain and a polypeptide comprising a C-lobe domain, wherein the N-lobe domain and C-lobe domain are not physically linked.
To source the polypeptides of the present disclosure any HIBP surface receptor protein, or TbpB polypeptide obtainable from or obtained from any Gram-negative bacterial species may be used, including, but not limited to, any pathogenic bacterial species or strain, and including, but not limited to, any bacterial species belonging to the bacterial families of Pasteurellaceae, Moxarellaceae or Neisseriaceae, and bacterial species belonging to the bacterial genus Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella. The polypeptides further include any polypeptides obtainable from or obtained from any HIBP surface receptor protein, or any polypeptides obtainable from or obtained from any TbpB polypeptide obtainable or obtained from the following bacterial species: Actinobacillus pleuropneumoniae (12, 34), Actinobacillus suis, Haemophilus influenzae (35, 36), Haemophilus parasuis (37), Haemophilus somnus (also known to the art as Histophilus somnus) (38), Mannheimia haemolytica (also known to the art as Pasteurella haemolytica) (39), Moraxella catarrhalis (40), Moraxella bovis, Neisseria gonorrhoeae, Neisseria meningitidis (41, 42), Mannheimia glucosida (also known to the art as Pasteurella haemolytica) and Bibersteinia trehalosi (also known to the art as Pasteurella trehalosi).
Exemplary C-lobe domain and N-lobe domain polypeptides that may be used in accordance herewith further include, any C-lobe domain set forth in SEQ.ID NO: 5; SEQ.ID NO: 6; SEQ.ID NO: 22; SEQ.ID NO: 33; SEQ.ID NO: 34; SEQ.ID NO: 119; SEQ.ID NO: 125; SEQ.ID NO: 179 to SEQ.ID NO: 195; SEQ.ID NO: 213 to SEQ.ID NO: 218; SEQ.ID NO: 230; SEQ.ID NO: 232; SEQ.ID NO: 234 to SEQ.ID NO: 278, and SEQ.ID NO: 288 to SEQ.ID NO: 292, and any N-lobe domain set forth in SEQ.ID NO: 8; SEQ.ID NO: 10; SEQ.ID NO: 24; SEQ.ID NO: 26; SEQ.ID NO: 36; SEQ.ID NO: 38; SEQ.ID NO: 121; SEQ.ID NO: 127; SEQ.ID NO: 229; SEQ.ID NO: 231; and SEQ.ID NO: 233, and further include any C-lobe domain or N-lobe domain that may be prepared from a HIBP polypeptide or a TbpB polypeptide including, without limitation the polypeptides set forth in SEQ.ID NO: 2; SEQ.ID NO: 12; SEQ.ID NO: 28; SEQ.ID NO: 107 to SEQ.ID NO: 115; SEQ ID NO: 117; SEQ.ID NO: 123; SEQ.ID NO: 131 to SEQ.ID NO: 147; SEQ.ID NO: 177; SEQ.ID NO: 178; SEQ.ID NO: 196 to SEQ.ID NO: 204; SEQ.ID NO: 206 to SEQ.ID NO: 212; and SEQ.ID NO: 219 to SEQ.ID NO: 228 or by using the nucleic acid sequences encoded by SEQ.ID NO: 1; SEQ.ID NO: 11; SEQ.ID NO: 27; SEQ.ID NO: 116; SEQ.ID NO: 122; and SEQ.ID NO: 173. Using these nucleic acid sequences and polypeptide sequences, additional novel HIBP surface receptor proteins and TbpB sequences, and C-lobe domains or N-lobe domains may readily be identified by those of skill in the art. For example expression libraries, cDNA libraries and genomic libraries may be screened and databases comprising sequence information may be searched for similar sequences.
The immunogenic preparations of the present disclosure elicit, upon administration thereof to a vertebrate subject, an immune response in such vertebrate subject, in the form of the stimulation of antibody production by the vertebrate subject. In accordance herewith, such antibodies are reactive against at least one Gram-negative bacterial strain. Preferably, however, the antibodies are cross-reactive and/or cross-protective against a plurality of bacterial strains or species, and preferably such cross-reactivity and/or cross-protection is attained in a host expressing one or more host iron binding proteins, such as transferrin or lactoferrin. The term “cross-reactive” as used herein refers to the ability of the immune response induced by an immunogenic composition obtained from one bacterial strain to stimulate the production of antibodies capable of additionally reacting with a different bacterial strain or different species. The term “cross-protective” as used herein refers to the ability of the immune response, induced by an immunogenic composition obtained from one bacterial strain, to prevent or attenuate infection or disease by at least one additional bacterial strain or bacterial species. In preferred embodiments of the present disclosure, the immunogenic compositions of the present disclosure are cross-reactive and/or cross-protective against a plurality of bacterial strains or bacterial species, for example, 2, 3, 4, 5, 6, 7, 8, 9 or 10 bacterial species or bacterial strains. Cross-reactivity is deemed to be an indicator of cross-protection. It will be readily appreciated by those of skill in the art that the foregoing aspect of the present disclosure facilitates vaccine manufacture by permitting the production of one immunogenic compound, i.e. an immunogenic compound obtainable from one HIBP surface receptor protein, which offers protection against multiple infectious bacterial strains or bacterial species.
The immunogenic preparations of the present disclosure generate an unexpectedly effective immune response in vertebrate subjects, and notably in vertebrate subjects expressing host iron binding proteins, such as transferrin and lactoferrin, exceeding the effectiveness of the immune response generated when immunizing preparations using native HIBP protein are used. One aspect of the effective immune response is the magnitude of the immune response. Preferably the antibody titre obtained using the immunogenic compositions of the present disclosure exceeds the antibody titre of a native HIBP protein by a factor of at least 2×, more preferably by a factor of at least 5×, most preferably by a factor of at least 10×.
In preferred embodiments, mixtures comprising at least two polypeptides, each polypeptide comprising or consisting of a C-lobe domain are used; or mixtures comprising at least two polypeptides, each polypeptide comprising or consisting of an N-lobe domain, are used; or mixtures comprising at least three polypeptides comprising or consisting of at least two C-lobe domains and at least one N-lobe domain, are used; or mixtures comprising at least three polypeptides comprising or consisting of at least two N-lobe domains and at least one C-lobe domain, are used. In preferred embodiments, the at least two polypeptides are obtainable or obtained from a Gram-negative bacterial genus or species capable of infecting the same vertebrate species. Thus, the at least two polypeptides would be selected from e.g. two C-lobe domains of an TbpB polypeptide, wherein both C-lobe domains are obtainable or obtained from a TbpB polypeptide obtained or obtainable from an Actinobacillus strain capable of infecting pigs, or e.g. two C-lobe domains of a TbpB polypeptide, wherein both C-lobe domains are obtainable or obtained from an TbpB polypeptide obtained or obtainable from a Haemophilus strain capable of infecting cows. In embodiments wherein at least two C-lobe domains are used, the mixtures are preferably free, or substantially free, of N-lobe domains or portions thereof. In embodiments wherein at least two N-lobe domains are used the mixtures are preferably free or substantially free of C-lobe domains or portions thereof.
In particularly preferred embodiments, in accordance with the present disclosure, mixtures of at least two polypeptides, each polypeptide comprising or consisting of a C-lobe domain of an HIBP surface receptor protein obtainable or obtained from a Gram-negative bacterial species are used. In such embodiments, the mixtures are preferably free from N-lobe domains or portions thereof. In particularly preferred embodiments, any two C-lobe domains obtainable or obtained from the HIBP surface receptor polypeptides set forth in SEQ.ID NO: 2; SEQ.ID NO: 12; SEQ.ID NO: 28; SEQ.ID NO: 107 to SEQ.ID NO: 115; SEQ ID NO: 117; SEQ.ID NO: 123; SEQ.ID NO: 131 to SEQ.ID NO: 147; SEQ.ID NO: 177; SEQ.ID NO: 178; SEQ.ID NO: 196 to SEQ.ID NO: 204; SEQ.ID NO: 206 to SEQ.ID NO: 212; and SEQ.ID NO: 219 to SEQ.ID NO: 228 are used; or any two C-lobe domains obtainable or obtained from the nucleic acid sequences set forth in SEQ.ID NO: 1; SEQ.ID NO: 11; SEQ.ID NO: 27; SEQ.ID NO: 116; SEQ.ID NO: 122; and SEQ.ID NO: 173 are used. In further preferred embodiments, any two C-lobe domains selected from the C-lobe domains set forth in SEQ.ID NO: 5; SEQ.ID NO: 6; SEQ.ID NO: 22; SEQ.ID NO: 33; SEQ.ID NO: 34; SEQ.ID NO: 119; SEQ.ID NO: 125; SEQ.ID NO: 179 to SEQ.ID NO: 195; and SEQ.ID NO: 213 to SEQ.ID NO: 218; SEQ.ID NO: 230: SEQ.ID NO: 232; SEQ.ID NO: 234 to SEQ.ID NO: 278; and SEQ.ID NO: 288 to SEQ.ID NO: 292 are used.
In further particularly preferred embodiments, at least two C-lobe domains obtainable or obtained from an HIBP surface receptor protein are used, wherein at least one C-lobe domain is obtainable or obtained from a bacterial species belonging to the bacterial genus of Actinobacillus, Haemophilus, Histophilus, Mannheimia, Moraxella, Neisseria, Pasteurella and Bibersteinia.
In further particularly preferred embodiments, at least two C-lobe domains are used, wherein at least one C-lobe domain is obtainable or obtained from an HIBP surface receptor protein obtainable or obtained from Actinobacillus pleuropneumoniae, preferably the C-lobe domain set forth in SEQ.ID. NO: 6 or SEQ.ID NO: 22, or wherein at least one C-lobe domain is obtainable or obtained from an HIBP surface receptor protein obtainable or obtained from Actinobacillus suis preferably the C-lobe domain set forth in SEQ.ID NO: 34.
In further particularly preferred embodiments, at least two C-lobe domains are used, wherein at least one C-lobe domain is obtainable or obtained from an HIBP surface receptor protein obtainable or obtained from Mannheimia haemolytica preferably the C-lobe domain set forth in SEQ.ID NO: 232; or SEQ.ID NO: 234,or wherein at least one C-lobe domain is obtainable or obtained from an HIBP surface receptor protein obtainable or obtained from Mannheimia glucosida.
In further particularly preferred embodiments, at least two C-lobe domains are used, wherein at least one C-lobe domain is obtainable or obtained from an HIBP surface receptor protein obtainable or obtained from Neisseria gonorrhoeae preferably one of the C-lobe domains set forth in SEQ.ID NO: 213 to SEQ.ID NO:,218, or wherein at least one C-lobe domain is obtainable or obtained from an HIBP surface receptor protein obtainable or obtained from Neisseria meningitidis preferably one of the C-lobe domains set forth in SEQ.ID. NO: 119; SEQ.ID NO: 125; SEQ.ID NO: 128; SEQ.ID NO: 129; SEQ.ID NO: 130; SEQ.ID NO: 131; SEQ.ID NO: 152; SEQ.ID NO: 154; SEQ.ID NO: 156; SEQ.ID NO: 158; SEQ.ID NO: 160; SEQ.ID NO: 164; SEQ.ID NO: 166; SEQ.ID NO: 168; SEQ.ID NO: 179 to SEQ.ID NO: 195; and SEQ.ID NO: 235 to SEQ.ID NO: 278.
In further particularly preferred embodiments, at least two C-lobe domains are used, wherein at least one C-lobe domain is obtainable or obtained from an HIBP surface receptor protein obtainable or obtained from Bibersteinia trehalosi, preferably the C-lobe domain set forth in SEQ.ID.NO: 292.
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a HIBP surface receptor polypeptide are used, wherein both of the two C-lobe domains are obtainable or obtained from two bacterial species selected from Actinobacillus pleuropneumoniae, preferably, the C-lobe domain set forth in SEQ.ID NO: 6 or SEQ.ID NO: 22, Actinobacillus suis, preferably the C-lobe domain set forth in SEQ.ID NO: 34 and Haemophilus parasuis, preferably the C-lobe domain set forth in SEQ.ID NO: 294.
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a HIPB surface receptor polypeptide are used, wherein one of the two C-lobe domains are obtainable or obtained from Neisseria gonorrhoeae, preferably, one of the C-lobe domains set forth in SEQ.ID. NO: 213 to SEQ.ID. NO:,218 and the other C-lobe domain is obtainable from Neisseria meningitidis preferably one of the C-lobe domains set forth in. SEQ.ID. NO: 119; SEQ.ID NO: 125; SEQ.ID NO: 128; SEQ.ID NO: 129; SEQ.ID NO: 130; SEQ.ID NO: 131; SEQ.ID NO: 152; SEQ.ID NO: 154; SEQ.ID NO: 156; SEQ.ID NO: 158; SEQ.ID NO: 160; SEQ.ID NO: 164; SEQ.ID NO: 166; SEQ.ID NO: 168; SEQ.ID NO: 179 to SEQ.ID NO: 195; and SEQ.ID NO: 235 to SEQ.ID NO: 278.
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a HIPB surface receptor polypeptide are used, wherein one of the two C-lobe domains is obtainable or obtained from Mannheimia haemolytica, preferably a C-lobe domain set forth in SEQ.ID NO: 232; or SEQ.ID NO: 234, and the other C-lobe is obtainable or obtained from Bibersteinia trehalosi preferably a C-lobe domain set forth in SEQ.ID NO: 292.
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB polypeptide are used, wherein both of the two C-lobe domains are obtainable or obtained from one or from two bacterial species, and wherein the TbpB polypeptides or the C-lobe domains obtained therefrom are antigenically divergent. The TbpB or C-lobe domains are preferably obtained from bacterial species or bacterial strains capable of exchanging TbpB variants. The term “antigenically divergent” as used herein in relation to two TbpB polypeptides or C-lobe domains of TbpB polypeptides means that the two TbpB polypeptides or C-lobe domains of TbpB polypeptides when used to construct a phylogenetic tree using a representative number of TbpB polypeptides or C-lobe domain polypeptides belong to divergent branches or groups of a phylogenetic tree. In accordance herewith, a phylogenetic tree with any amount of TbpB or C-lobe domain polypeptides may be constructed, however preferably a phylogenetic tree is constructed using at least 25 TbpB polypeptides or C-lobe domain polypeptides, more preferably at least 30, at least 40, or at least 50 TbpB polypeptides or C-lobe domain polypeptides, and preferably a phylogenetic tree is constructed in such a manner that it comprises at least 2 node orders above the root level, more preferably a phylogenetic tree comprises at least 3, 4, or 5 node orders above the root level, most preferably, at least 6, 7, 8, 9, or 10 node orders above the root level (as further explained below and in
Now referring to
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB polypeptide are used, wherein both of the two C-lobe domains are obtainable or obtained from Actinobacillus pleuropneumoniae, Actinobacillus suis and Haemophilus parasuis. Referring to the phylogenetic tree set forth in
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB protein are used, wherein both of the two C-lobe domains are obtainable or obtained from Neisseria meningitidis. Referring to the phylogenetic tree set forth in
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB polypeptide are used, wherein one of the two C-lobe domains is obtainable or obtained from Neisseria meningitidis and the other of the C-lobes is obtainable or obtained from Neisseria gonorrhoeae. Referring to the phylogenetic tree set forth in
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB polypeptide are used, wherein one of the two C-lobe domains is obtainable or obtained from Neisseria meningitidis and the other of the C-lobes is obtainable or obtained from Neisseria gonorrhoeae. Referring to the phylogenetic tree set forth in
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB protein are used, wherein both of the two C-lobe domains are obtainable or obtained from Haemophilus influenzae. Referring to the phylogenetic tree set forth in
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB protein are used, wherein both of the two C-lobe domains are obtainable or obtained from Mannheimia haemolytica. Referring to the phylogenetic tree set forth in
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB polypeptide are used, wherein one of the two C-lobe domains is obtainable or obtained from Bibersteinia trehalosi and the other of the C-lobes is obtainable or obtained from Mannheimia haemolytica. Referring to
In further preferred embodiments, at least two C-lobe domains obtainable or obtained from a TbpB protein are used, wherein both of the two C-lobe domains are obtainable or obtained from Moraxella catharrhalis. Referring to the phylogenetic tree set forth in
In particularly preferred embodiments, the aforementioned mixtures of polypeptides comprising or consisting of C-lobe domains are C-lobe domains obtainable or obtained from TbpB polypeptides, including, without limitation the C-lobe domains set forth in SEQ.ID NO: 5; SEQ.ID NO: 6; SEQ.ID NO: 22; SEQ.ID NO: 33; SEQ.ID NO: 34; SEQ.ID NO: 119; SEQ.ID NO: 125; SEQ.ID NO: 179 to SEQ.ID NO: 195; and SEQ.ID NO: 213 to SEQ.ID NO: 218; SEQ.ID NO: 230; SEQ.ID NO: 232; and SEQ.ID NO: 234 to SEQ.ID NO: 278.
The foregoing mixtures of C-lobe domains may be prepared by mixing preparations comprising the individual C-lobe domains or by recombinantly producing fusion polypeptides comprising two or more C-lobe domains.
As hereinbefore mentioned, the immunogenic preparations of the present disclosure are preferably cross-reactive and/or cross-protective. While, as hereinbefore mentioned, formulations comprising single C-lobe domains may be cross-reactive and/or cross-protective, mixtures of C-lobe domains are particularly preferred, in that they may be used to prepare immunogenic formulations that substantially broaden cross-reactivity and/or cross-protection against a wider range of bacterial strains and/or species, and allow the preparation of vaccine formulations providing protection from infection or disease transmitted by a plurality of bacterial species or bacterial strains.
In accordance with other embodiments, a polypeptide comprising a C-lobe domain and/or an N-lobe domain of an HIBP surface receptor polypeptide is prepared in such a manner that a loop domain connecting two β-strands within the C-lobe domain or the N-lobe domain is modified and the polypeptide is unable to substantially bind host iron binding protein. The term “modified” as used herein in conjunction with a loop domain refers to a loop from which at least one amino acid residue has been removed or replaced. Thus the resultant loop within the C-lobe domain or the N-lobe domain may be truncated or, in other embodiments the amino acid residue, residues may be replaced with one or more alternate amino residues.
In further preferred embodiments, amino acid residues within the loop domains of the C-lobe domain and/or N-lobe domain are replaced by others e.g. by site directed mutagenesis, the resulting polypeptide comprising a modified C-lobe domain or N-lobe domain and unable to substantially bind host iron binding protein.
The reductions in size of one or more loop domains, or modification of amino acids in the loop domain of the HIBP polypeptides in accordance with the present disclosure are preferably made in such a manner that the resultant polypeptide is conformationally stable. By the term “conformationally stable” it is meant that the conformational state or conformation of the polypeptide remains substantially the same following the modification in size of the loop or replacement of amino acid residue. The conformational state of the loop domain which is modified may be more or less altered. The determinants of conformational state or confirmation of a polypeptide include: the polypeptide's primary structure as reflected in its amino acid sequence, the polypeptide's secondary structure (e.g. α-helix, (-sheet and the like), the polypeptide's tertiary structure (i.e. the three dimensional folding of the polypeptide chain) and the quarternary structure (i.e. the interaction of the polypeptide with other protein subunits). Protein conformation can further be influenced by environmental factors, such as pH, osmolarity, ionic strength and salt concentration. The design of loop reduction may be informed by the alignment and comparison of multiple heterologous sequences, the comparison of three-dimensional conformational structures of multiple heterologous polypeptides known to the art, and the use of conservative amino acid substitutions (e.g. combinations such as gly, ala; val, ile; leu, met; asp, glu; asn, gln; ser, thr; lys, arg; cys, met; and phe, trp, tyr). Furthermore, the conformational state of a protein may be assayed by a functional assay (e.g. binding of a host iron binding protein), or by physical methods such as X-ray crystallography or Nuclear Magnetic Resonance (NMR).
In further embodiments, at least one loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the longest loop, is selected to be modified. In embodiments, wherein such a loop is modified in its entirety this generally will involve the removal of at least 25 amino acid residues and may result in the removal of 150 amino acid residues or more.
In preferred embodiments, at least one loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the longest loop (i.e. comprising the most amino acid residues) of the C-lobe domain or the N-lobe domain is modified. In a further preferred embodiment, at least one loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the longest loop of the C-lobe domain or the N-lobe domain is modified, and a second loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the second longest loop are selected to be modified. In further preferred embodiments, at least one loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the longest loop, and a second loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the second longest loop are selected to be modified and a third loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the third longest loop are selected to be modified. In yet further preferred embodiments, at least one loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the longest loop, and a second loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the second longest loop are selected to be modified and a third loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the third longest loop are selected to be modified, and a fourth loop domain of the C-lobe domain or the N-lobe domain of an HIBP-surface receptor protein comprising the fourth longest loop are selected to be modified. The aforementioned embodiments are further detailed in Examples 3 and 4 hereto.
Surprisingly, in accordance herewith, it has been found that a polypeptide substantially consisting of a C-lobe domain or an N-lobe domain of a HIBP surface receptor protein can readily be produced, for example in a microbial production system, when one or more loop domains are modified, and the modified polypeptide is substantially conformationally stable.
In accordance herewith, the modified C-lobe domain or the N-lobe domain polypeptide may be used per se as an immunogen, or the polypeptide may be modulated to comprise further modifications. Modifications to the modified C-lobe domain or N-lobe domain of the polypeptide that may be made in accordance herewith include the preparation of N-terminal or C-terminal polypeptide extensions of the native or the modified C-lobe domain or N-lobe domain polypeptide. Such N-terminal and C-terminal polypeptide extensions include the addition of a second full length C-lobe domain polypeptide to the C-lobe domain, thus providing a C-lobe domain dimer, the addition of a second full length N-lobe domain polypeptide to the N-lobe domain, thus providing an N-lobe domain dimer, or an addition comprising a portion of a C-lobe domain polypeptide or a portion of an N-lobe domain polypeptide. Multimers may be assembled using the same monomeric polypeptide (i.e. homodimers, homotrimers etc.), or they may be assembled using different polypeptides, e.g. a C-lobe domain or an N-lobe domain obtained from different variants (i.e. hetrodimers, heterotrimers etc.). In preferred embodiments, heteromultimeric proteins representing different pathogens or pathogenic strains are assembled. Thus in one preferred embodiment, a heteromultimeric polypeptide comprising C-lobe domains or N-lobe domains selected from the group consisting of Actinobacillus pleuropneumoniae, Actinobacillus suis and Haemophilus parasuis C-lobe domains or N-lobe domains is prepared. In particularly preferred embodiments, the C-lobe domains or N-lobe domains are selected from the group consisting of A. pleuropneumoniae H49, A. suis H57 and A. pleuropneumoniae H87 C-lobe domains or N-lobe domains. In a further preferred embodiment, a heteromultimeric polypeptide comprising C-lobe domains selected from at least two TbpB C-lobe domains or N-lobe domains selected from strains of Neisseria meningitidis is prepared. In particularly preferred embodiments, the strains are selected from N. meningitidis M982 or N. meningitidis B16B6. Heteromultimeric proteins may convey immunogenicity to different pathogens. In further preferred embodiments the present disclosure provides, (i) a first polypeptide, comprising an N-lobe domain or a C-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a Gram-negative pathogenic bacterial species, wherein the N-lobe domain or the C-lobe domain comprise a plurality of 3-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains of the N-lobe domain or the C-lobe domain has been modified, linked to (ii) a second polypeptide comprising an HIBP surface receptor protein, or a portion thereof, obtainable from a Gram-negative bacterial species. In preferred embodiments, the portion of the HIBP surface receptor protein is an N-lobe domain or a C-lobe domain. In further preferred embodiments, the portion of the HIBP surface protein is an N-lobe domain or a C-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a Gram-negative pathogenic bacterial species, wherein the N-lobe domain or the C-lobe domain comprise a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains of the N-lobe domain or the C-lobe domain has been modified.
In further embodiments, a multimeric polypeptide is prepared, such multimeric protein comprising a plurality of N- and C-terminal extensions, including the addition of a second, third, fourth, fifth, sixth or seventh full length C-lobe domain polypeptide to the C-lobe domain, thus providing a C-lobe domain multimer, or the addition of a second, third, fourth, fifth, sixth or seventh, full length N-lobe domain polypeptide to the N-lobe domain, thus providing an N-lobe domain dimer, or an addition comprising a portion of a C-lobe domain polypeptide or a portion of an N-lobe domain polypeptide. Thus for example, in one embodiment, the C-lobe domains are at least two, or at least three, C-lobe domains obtainable from a TbpB polypeptide obtainable from A. pleuropneumoniae, A. suis and Haemophilus parasuis. In accordance with such embodiment, the nucleic acid sequences encoding the TbpB C-lobe domains from A. pleuropneumoniae H49 (SEQ.ID NO: 5), A. suis H57 (SEQ.ID NO: 33) and A. pleuropneumoniae H87 (SEQ.ID NO: 21) may be linked to form a chimeric nucleic acid sequence (SEQ.ID NO: 39) encoding a single polypeptide (SEQ.ID NO: 40) encompassing the three C-lobes (SEQ.ID NO: 6; SEQ.ID NO: 34; SEQ.ID NO: 22). Accordingly, in yet further embodiments, the present disclosure provides (i) a first polypeptide, comprising an N-lobe domain or a C-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a Gram-negative pathogenic bacterial species, wherein the N-lobe domain or the C-lobe domain comprise a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains of the N-lobe domain or the C-lobe domain has been modified, linked to (ii) a plurality of polypeptides each polypeptide comprising an HIBP surface receptor protein, or a portion thereof, obtainable from a Gram-negative bacterial species. In preferred embodiments the portion of the HIBP surface receptor protein is an N-lobe domain or a C-lobe domain. In further preferred embodiments, the portion of the HIBP surface protein is an N-lobe domain or a C-lobe domain of an HIBP surface receptor protein obtainable from or obtained from a Gram-negative pathogenic bacterial species, wherein the N-lobe domain or the C-lobe domain comprise a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains of the N-lobe domain or the C-lobe domain has been modified.
In further embodiments, upon removal of one or more amino acids from one or more loop domains of the C-lobe or N-lobe of an HIBP surface receptor protein, these residues are replaced with one or more alternate amino acid residues. In one embodiment, the alternate amino acid residues comprise a heterologous polypeptide antigenic determinant capable of eliciting an immune response in a vertebrate host organism. In further embodiments the alternate amino acid residues comprise two or more heterologous polypeptide antigenic determinants capable of eliciting an immune response in a vertebrate host organism. The heterologous antigenic determinants may be immuno-cross-reactive to the same or a different pathogenic organism. Thus it will be clear that the modified C-lobe domain or N-lobe domain of an HIBP surface receptor protein in accordance herewith may be used as a scaffold to produce and present one or more antigenic determinants. In a preferred embodiment, one or more loop regions of the C-lobe domain or the N-lobe domain of TbpB is replaced with one or more polypeptide portions obtainable from or obtained from an IOM protein, including a transferrin binding protein (“TbpA”) or lactoferrin binding protein A (“LbpA”). LbpA and TbpA polypeptides that may be used in accordance herewith include those set forth in SEQ.ID NO: 162 and SEQ.ID NO: 152. Portions of LbpA and TbpA polypeptides that may be used in accordance herewith include those set forth in SEQ.ID NO: 286 and SEQ.ID NO: 287. These fragments may be used to construct chimeric nucleic acid sequences and polypeptides, including those set forth in SEQ.ID. NO: 163; SEQ.ID. NO: 164; SEQ.ID. NO: 165; SEQ.ID. NO: 166, SEQ.ID. NO: 167; and SEQ.ID. NO: 168. This embodiment of the disclosure is further detailed in Examples 7 and 8. hereto. In a further preferred embodiment, one or more loop domains of the C-lobe or N-lobe of TbpB is replaced with a lysine rich polypeptide sequence, as further described in Example 9.
In all of the hereinbefore described embodiments which comprise an HIBP surface receptor polypeptide comprising a N-lobe domain, it is preferable that, the N-lobe domain is modified in such a manner that the N-terminal anchor polypeptide, or a substantial portion thereof, is removed from the N-lobe domain. The length of the anchor polypeptide may vary depending on the HIBP surface receptor polypeptide, but typically ranges from 40 to 75 amino acids in length, and located at the N-terminal end of the mature HIPB polypeptide. Thus referring to
As hereinbefore mentioned, in accordance with the present disclosure the HIBP surface receptor protein is modified in such a manner that it is unable to substantially bind host iron binding protein. The binding of host iron binding protein to the modified HIBP surface receptor protein may be evaluated using any chemical or biochemical assay capable of evaluating such binding including, for example, a solid phase binding assay, an affinity capture assay, or a biophysical assay. General methodologies to conduct these assays are known to those of skilled in the art and described in for example (12, 23-25, 50, 51). By conducting these assays the difference in binding characteristics between the native HIBP surface receptor protein and the native host iron binding protein, and the modified HIBP surface receptor protein and the native host iron binding protein, may readily be determined, and a series of modified HIBP surface receptor proteins may be evaluated in order to determine whether they are able to bind host iron binding protein. Different binding characteristics may be determined, including the binding constant (Kd). As hereinbefore mentioned, the Kd characterizing the binding between the host iron binding protein and the modified HIBP surface receptor proteins of the present disclosure is at least 2× higher than the Kd characterizing the binding between the host iron binding protein and the native HIBP surface receptor protein. One exemplary assay method to determine the binding constant using host transferrin is further described in Example 11 hereto.
The present disclosure further includes a method of identifying a modified HIBP surface receptor protein, the method comprising:
“Substantially modulated” as used herein means that the binding interaction forces between the modified HIBP surface receptor proteins and the host iron binding protein are substantially weaker than the binding interaction forces between the native HIBP surface receptor proteins and the host iron binding protein. In preferred embodiments, the binding characteristic that used is the Kd relating to the binding interaction of the HIBP surface receptor protein and the host iron binding protein, wherein the value of the Kd of the binding interaction between the modified HIBP surface receptor proteins and the host iron binding protein is at least 2× larger in value than the Kd of the binding interaction between the native HIBP surface receptor proteins and the host iron binding protein. It is further noted that foregoing method may be used to screen a plurality of different candidate HIBP surface receptor proteins, simultaneously or sequentially, and identifying among the screened candidate HIBP surface receptor proteins, those exhibiting a more or less pronounced modulation in binding characteristics relative to the native HIBP surface receptor proteins.
The present disclosure further includes a method of preparing a modified HIBP surface receptor protein for use as a vaccine, the method comprising:
In accordance with the foregoing, the identified HIBP surface receptor protein exhibiting substantially modulated binding characteristics relative to the binding characteristics of the native HIBP surface receptor proteins may be used to prepare immunogenic formulations, e.g. by recombinantly producing the HIBP surface receptor protein, isolating the HIBP surface protein, and preparing a vaccine formulation comprising the HIBP surface receptor protein.
In further embodiments, the present disclosure comprises methods of evaluating cross-reactivity of antisera against surface receptor protein variants. Accordingly the present disclosure further comprises a method of evaluating the cross-reactivity of antisera against surface receptor protein variants, the method comprising:
The nucleic acid sequence encoding the peptide susceptible to biotinylation additionally may comprise a nucleic acid sequence of sufficient length to permit, upon expression, a polypeptide extension for the fusion polypeptide such that the surface receptor protein is distant from the immunoassay substrate material and thereby fully accessible to binding of an antibody. The streptavidin coated immunoassay substrate material may be any substrate material, including, for example, an ELISA plate.
In further embodiments, the present disclosure comprises methods of evaluating the cross-reactive or protective properties of antiserum surface receptor protein variants, the method comprising:
The in-vivo or in-vitro immune assay may be any assay including any ELISA assay, functional immunological assay or animal infection model.
In yet further embodiments, the present disclosure comprises a method of evaluating the efficacy of a vaccine for the prevention of colonization of the mammalian upper respiratory tract by a Gram-negative bacterial strain expressing a surface receptor protein variant, the method comprising:
In general, it will be understood by a person of ordinary skill in the art, having read the current disclosure, that in accordance with the disclosure a series of different modulated polypeptides may be prepared and obtained, all of which, are modified HIBP surface receptor proteins, wherein the modification is made in such a manner that the modified HIBP surface receptor protein is unable to substantially bind host iron binding protein. These modulated polypeptides and methods of making such modulated polypeptides are all intended to be included within the scope of the compositions and methods herein provided.
The modified C-lobe domain or N-lobe domain polypeptides are conveniently prepared by providing a nucleic acid sequence encoding an HIBP surface receptor protein, and modulating the native nucleic acid sequence in such a manner that the polypeptides comprising the modified C-lobe domain or N-lobe domain are expressed in a recombinant host organism, for example a microbial cell. Modulations to the nucleic acid sequence may be made using a variety of nucleic acid modification techniques that will be generally known to those skilled in the art, including for example site directed mutagenesis, targeted mutagenesis, random mutagenesis, the addition of organic solvents, gene shuffling or a combination of these and other techniques known to those of skill in the art, each methodology designed to target the loop domains of the C-lobe domain or N-lobe domain in such a manner that a loop domain therein is modified. Alternatively the modulated nucleic acid sequences encoding the modified in size-C-lobe domain or N-lobe domain polypeptides may be prepared ab initio using gene synthesis techniques. General techniques to prepare and modify nucleic acid sequences are readily available to the skilled artisan, for example in Green and Sambrook, Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory Press, 2012, (33).
In other embodiments of the present disclosure, methods for preparing an immunogenic composition are provided. Accordingly, the present disclosure provides a method for preparing an immunogenic composition comprising:
In certain embodiments the C-lobe domain or N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains has been modified, and wherein the polypeptide has been modified in such a manner that it is unable to substantially bind host iron binding protein.
In further preferred embodiments the present disclosure provides a method for preparing an immunogenic composition comprising:
In preferred embodiments, the first and second nucleic acids are operably linked in such a manner that a heteromultimeric fusion polypeptide comprising a first and second C-lobe domain or a first and second N-lobe domain are produced, and wherein the heteromultimeric fusion polypeptide is unable to substantially bind host iron binding protein.
In accordance herewith, the nucleic acid sequence encoding the HIBP surface receptor protein is linked to a nucleic acid sequence capable of controlling expression of the HIBP surface receptor protein in a host cell. Accordingly, the present disclosure also provides a nucleic acid sequence encoding the HIBP surface receptor protein linked to a promoter capable of controlling expression in a host cell. Nucleic acid sequences capable of controlling expression in host cells that may be used herein include any transcriptional promoter capable of controlling expression of polypeptides in host cells. Generally, promoters obtained from bacterial cells are used when a bacterial host is selected in accordance herewith, while a fungal promoter will be used when a fungal host is selected, a plant promoter will be used when a plant cell is selected, and so on. Further nucleic acid elements capable of controlling expression in a host cell include transcriptional terminators, enhancers and the like, all of which may be included in the chimeric nucleic acid sequences of the present disclosure.
In accordance with the present disclosure, the chimeric nucleic acid sequences comprising a promoter capable of controlling expression in a host cell linked to a nucleic acid sequence encoding a polypeptide comprising a C-lobe domain or N-lobe domain of an HIBP surface receptor protein obtainable from a Gram-negative bacterial species, wherein the C-lobe domain or N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains has been modified, can be integrated into a recombinant expression vector which ensures good expression in the host cell. Accordingly, the present disclosure includes a recombinant expression vector comprising as operably linked components:
Pursuant to the present disclosure, the expression vector may further contain a marker gene. Marker genes that may be used in accordance with the present disclosure include all genes that allow the distinction of transformed cells from non-transformed cells, including all selectable and screenable marker genes. A marker gene may be a resistance marker such as an antibiotic resistance marker against, for example, kanamycin or ampicillin. Screenable markers that may be employed to identify transformants through visual inspection include, β-galactosidase, β-glucuronidase (GUS) (U.S. Pat. Nos. 5,268,463 and 5,599,670) and green fluorescent protein (GFP) (52).
One host cell that particularly conveniently may be used is Escherichia coli. The preparation of the E. coli vectors may be accomplished using commonly known techniques such as restriction digestion, ligation, ligation independent cloning, gel electrophoresis, DNA sequencing, the Polymerase Chain Reaction (PCR), and other methodologies. A wide variety of cloning vectors are available to perform the necessary steps required to prepare a recombinant expression vector including custom vectors that the inventors have developed. Among the vectors with a replication system functional in E. coli, are vectors such as the pUC or pET series of vectors, etc. Typically, these cloning vectors contain a marker allowing selection of transformed cells. Nucleic acid sequences may be introduced in these vectors, and the vectors may be introduced in E. coli by preparing competent cells, electroporation or using other well known methodologies to a person of skill in the art. E. coli may be grown in an appropriate medium, such as Luria-Broth medium and harvested. Recombinant expression vectors may readily be recovered from cells upon harvesting and lysing of the cells. Further, general guidance with respect to the preparation of recombinant vectors and growth of recombinant organisms may be found in, for example: Sambrook et al., Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory Press, 2001, Third Ed (33).
The production of the recombinant proteins can occur throughout the growth of the E. coli strain, preferably by induction of expression after a period of growth to achieve a significant biomass. This will result in the production of the polypeptide comprising the C-lobe domain or N-lobe domain or the C-lobe domain or N-lobe domain with modified loops. The polypeptide subsequently may be recovered, isolated and separated from other host cell components by a variety of different protein purification techniques including, e.g. metal-chelate chromatography, ion-exchange chromatography, size exclusion chromatography, affinity chromatography, hydrophobic interaction chromatography, reverse phase chromatography, gel filtration, etc. Further general guidance with respect to protein purification may for example be found in: Protein Purification: Principles, High Resolution Methods, and Applications (53). The term “recovered” as used herein means that the polypeptides is obtained in more or less pure form. In preferred embodiments, a substantially immunogenic polypeptide comprising a C-lobe domain of an HIBP surface receptor protein obtainable from a Gram-negative bacterial species, wherein the C-lobe domain or N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains has been modified, may be obtained in accordance herewith. Thus the HIBP polypeptides obtained in accordance herewith may be prepared in substantially pure form. By “substantially pure” it is meant that the immunogenic protein is separated from other host cell components. In accordance here with the immunogenic protein is at least 95% pure, and more preferably at least 96%, 97%, 98% or 99% pure. Alternatively, relatively crude fractions comprising the HIBP polypeptide may be obtained, e.g. cells containing the polypeptides, cell lysates containing the polypeptides, or cellular fractions containing the polypeptide.
In further embodiments, the present disclosure provides methods for eliciting an immune response in a vertebrate subject. The immune response may be elicited by the delivery of the immunogenic protein or by the delivery of an expression vector comprising a nucleic acid sequence encoding the immunogenic protein. Accordingly, the present disclosure further provides a method for eliciting an immune response in a vertebrate subject, said method comprising administering to the subject:
The present disclosure also provides a use of:
The present disclosure further provides a use of:
The present disclosure yet also provides:
In preferred embodiments, the polypeptide comprises at least two C-lobe domains, or at least two N-lobe domains. In further preferred embodiments, the polypeptide comprises at least three C-lobe domains, or at least three N-lobe domains.
In certain embodiments, the C-lobe domain or N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains has been modified in such a manner that the C-lobe domain or N-lobe domain is unable to substantially bind host iron binding protein.
The present disclosure further includes an immunogen comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor polypeptide wherein the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain has been modified for use as a medicament.
The present disclosure further includes an immunogen comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor polypeptide wherein the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain has been modified for use in the prevention of infection or disease by infectious Gram-negative bacteria, including bacteria belonging to the genus Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella.
The present disclosure further includes an immunogen comprising a C-lobe domain or an N-lobe domain of an HIBP surface receptor polypeptide wherein the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain has been modified for use in the manufacture of a medicament for the prevention of infection or disease by infectious Gram-negative bacteria, including bacteria belonging to the genus Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella.
The present disclosure further provides vaccine preparations. Thus, the present disclosure further provides a vaccine composition comprising an antigen derived from a HIBP surface receptor protein from a Gram-negative pathogenic bacterial species, wherein the protein derived from the HIBP surface receptor protein has been modified in such a manner that it is unable to substantially bind host iron binding protein. The vaccine compositions of the present disclosure preferably comprise a vaccine comprising a polypeptide comprising, an HIPB surface receptor protein, a C-lobe domain or N-lobe domain of an HIBP surface receptor protein obtainable from a Gram-negative pathogenic bacterial species wherein the polypeptide is modified in such a manner that it is unable to substantially bind host iron binding protein. In preferred embodiments, the vaccine preparation comprises a mixture of C-lobe domains belonging to two different bacterial species or two different bacterial strains. In further preferred embodiments, the polypeptide comprises at least two or at least three N-lobe domains or C-lobe domains. In further preferred embodiments, said at least two or at least three N-lobe domains or C-lobe domains form a heteromultimer. In further embodiments, the C-lobe domain or N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain of the plurality of loop domains has been modified.
The vaccine preparations of the present disclosure comprise the immunogenic HIBP polypeptides in more or less pure form. Thus, in accordance herewith, substantially pure HIBP polypeptides may be obtained and used to prepare vaccine formulations. In other embodiments more crude HIPB polypeptide preparations may be obtained and used to prepare vaccine formulations. Thus, for example, in such embodiments cells, cell lysates or cell fractions comprising the HIBP polypeptides may be used to prepare the vaccine formulations.
In order to augment an immune response in a subject, the compositions provided herein further preferably include adjuvants, such as pharmacological agents, cytokines, or the like. Suitable adjuvants include any substance that enhances the immune response of the subject to the immunogenic polypeptides of the disclosure. Non-limiting examples of adjuvants include cytokines, e.g., IL-1, IL-2, IL-12, IL-6, and further include inorganic salts, e.g. aluminum hydroxide, aluminum phosphate, and calcium phosphate; oil emulsions, eg. mineral oil, MF59, QS-21, Montamide ISA51 and ISA-720; Isocoms, eg. ISCOMATRIX; microbial derivatives, eg. MPLA, macrophage-activating protein-2, virosomes, LT/CT, CpG; natural polymers, eg. polysaccharides; and synthetic polymers, eg. polyanhydrides and polyesters, as reviewed in Wilson-Welder et al. (54). Adjuvants may be administered, for example, as proteins or other macromolecules at the same time, prior to, or subsequent to, administration of the polypeptide antigens.
Doses for immunogenic proteins, generally range from about 0.1 μg to about 20 mg, preferably 10 μg to about 3 mg for human subjects. The exact amount necessary, however, will vary depending on the age and general condition of the recipient subject to be treated, the severity of the condition being treated, the particular preparation delivered, the site of administration, as well as other factors. An appropriate therapeutically effective amount can be readily determined by one of skill in the art. Thus, a “therapeutically effective amount” of the present compositions will be an amount sufficient to bring about treatment or prevention of disease or condition symptoms, or to prevent colonization by the pathogenic bacteria, and will fall in a relatively broad range that can be determined through routine trials.
Vaccine preparations comprising the immunogenic composition of the present disclosure preferably further comprise vehicles, excipients and auxiliary substances, such as wetting or emulsifying agents, pH buffering substances and the like, may be present in the excipient or vehicle. These vehicles, excipients and auxiliary substances are generally pharmaceutical agents that do not induce an immune response in the recipient subject, and may be administered without undue toxicity. Pharmaceutically acceptable excipients include, but are not limited to, liquids such as water, saline, polyethyleneglycol, hyaluronic acid, glycerol and ethanol. Pharmaceutically acceptable salts can also be included therein, for example, mineral acid salts such as hydrochlorides, phosphates, sulfates, and the like; and the salts of organic acids such as acetates, propionates, benzoates, and the like. It is also preferred, although not required, that the preparation will contain a pharmaceutically acceptable excipient that serves as a stabilizer, particularly in order to stabilize the polypeptides of the present disclosure. Examples of suitable carriers that also act as stabilizers for peptides include, without limitation, pharmaceutical grades of dextrose, sucrose, lactose, sorbitol, inositol, dextran, and the like. Other suitable carriers include, again without limitation, starch, cellulose, sodium or calcium phosphates, citric acid, glycine, polyethylene glycols (PEGs), and combinations thereof.
The vaccine preparations of the present disclosure may be used to prevent infection or disease caused by infectious Gram-negative bacteria, including, without limitation, bacteria belonging to the genus Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella, including without limitation Actinobacillus pleuropneumoniae, Actinobacillus suis, Haemophilus parasuis, Neisseria meningitidis. The vaccine preparations may be used to immunize any vertebrate subject including any vertebrate subject expressing host iron binding proteins and including, without limitation, any mammalian subject, including any human subject, porcine subject, bovine subject, equine subject, ovine subject, hircine subject, canine subject, feline subject, leporine subject and, further any ruminant subject, and murine subject. Other vertebrate subjects that may be immunized include any avian subject and piscine subject. The vaccine preparations of the present disclosure may exhibit an enhanced cross-reactive and/or cross-protective immunological response in the recipient host organism. It is further noted that the vaccine preparations of the present disclosure may prevent infection and/or colonization by the infectious Gram-negative bacteria, including bacterial colonization of the respiratory tract or genital tract of a vertebrate subject.
The present disclosure further includes a vaccine comprising an HIPB surface receptor protein, a C-lobe domain or an N-lobe domain of an HIBP surface receptor polypeptide wherein the C-lobe domain or the N-lobe domain comprises a plurality of β-strands connected by a plurality of loop domains, and wherein at least one loop domain has been modified for use in the prevention of infection or disease by infectious gram-negative bacteria, including bacteria belonging to the genus Actinobacillus, Neisseria, Haemophilus, Mannheimia, Histophilus, Pasteurella or Moraxella.
In accordance herewith the efficacy of the vaccine preparations of the present disclosure may be evaluated, for example by determining the antibody titre present in blood serum of subjects immunized with a vaccine preparation, e.g. by the performance of an Enzyme Linked Immuno Sorbent Assay (ELISA). Accordingly the present disclosure further comprises a method for evaluating the efficacy of a vaccine preparation comprising a C-lobe domain or N-lobe domain of an HIBP surface receptor protein obtainable from a Gram-negative pathogenic bacterial species wherein the polypeptide is modified in such a manner that it is unable to substantially bind host iron binding protein, the method comprising:
The vertebrate blood serum may be assayed following the administration of single or multiple (e.g. 2, 3, or 4) doses of a vaccine preparation. Assays, such as ELISA assays, may be performed using HIBP surface receptor protein isolates from a single or multiple microbial different strains or species. ELISA assays may involve linking of the HIBP surface receptor polypeptide to a carrier protein, such as a maltose binding protein. Where the reactivity of antibodies against multiple HIBP surface receptor protein isolates is assayed, it is possible to evaluate the vaccine preparation for cross-reactivity.
As is apparent to those skilled in the art in view of the teachings of this specification, vaccination with the above-described polypeptides or with nucleic acid sequences encoding such polypeptides (DNA vaccines) can be effected in one dose, continuously or intermittently throughout the course of treatment. Methods of determining the most effective means and dosages of administration are well known to those of skill in the art and will vary with the delivery vector, the nature of the composition, the specific prophylaxis or therapy sought, the target cells, and the subject being treated. Single and multiple administrations can be carried out with the dose level and pattern being selected by suitable medical personnel.
Administration of the above-described pharmaceutical preparations can be effected in one dose, continuously or intermittently throughout the course of treatment. Delivery will most typically be via conventional needle and syringe for the liquid compositions and for liquid suspensions containing particulate compositions. In addition, various liquid jet injectors are known in the art and may be employed to administer the present compositions. The route of vaccine delivery may vary. Thus the vaccines of the present disclosure may be delivered intravenously, subcutaneous, intramuscular, intravaginal, intraperitoneal, intranasal, oral or via other mucosal routes. Methods of determining the most effective means and dosages of administration are well known to those of skill in the art and will vary with the delivery vehicle, the composition of the therapy, the target cells, and the subject being treated. Single and multiple administrations can be carried out with the dose level and pattern being selected by the attending physician or veterinarian.
Below are examples of specific embodiments for carrying out the present disclosure. The examples are offered for illustrative purposes only, and are not intended to limit the scope of the present disclosure in any way.
This Example provides an illustration of the value of using sub-domains of the TbpB receptor proteins to obtain a more desirable immune response. In regards to what we mean by a more desirable immune response we consider both the magnitude of the antibody response and the cross-reactivity of the antibodies with variant TbpB proteins.
The second experiment (
The third experiment illustrated in this example (
The results in
Surprisingly, the enhanced cross-reactivity induced by the C-lobe domain was retained even when the C-lobe was mixed with the N-lobe in the immunizing mixture (
The results also suggest that there is a reduced response against the intact TbpB and TbpB N-lobe relative to the C-lobe (
It is important to mention that in contrast to previous published studies we did not use the standard ELISA method for measuring antibody levels since we identified a major deficiency in the standard ELISA method. We observed that in contrast to what is commonly assumed, purified proteins do not necessarily bind randomly to the ELISA plates, providing potentially strong biases or deficiencies in evaluating the binding of antibodies to epitopes on the surface of the protein. In particular we noted that TbpB, and particularly the N-lobe of TbpB, essentially binds to the solid surface of ELISA plates in one orientation, masking the Cap region of the N-lobe so that the binding of transferrin cannot be detected (
To overcome this deficiency we devised a method for binding recombinant proteins to streptavidin-coated ELISA plates by virtue of a biotin residue that was enzymatically added to the N-terminus during protein expression in the cytoplasm. As shown in
The recombinant antigens for the immunization experiments were produced in the cytoplasm of E. coli using a custom T7 expression vector encoding an N-terminal polyhistidine tag, a maltose binding protein and a tobacco etch virus (TEV) protease cleavage site. The recombinant fusion proteins were isolated by Ni-NTA chromatography, the antigens released by (TEV) cleavage and purified by a combination of Ni-NTA and Q-Sepharose chromatography.
FVB mice (albino MHC haplotype H2q from Charles River), 3 month-old New Zealand White rabbits and 51 day-old Large White Landrace F1 cross pigs were used for the immunization experiment in
Sera taken at week 8 were tested against representative proteins in our custom solid-phase ELISA assay. The recombinant fusion proteins used in the ELISA assay were produced in the cytoplasm of E. coli using a custom T7 expression vector encoding an N-terminal optimized biotinylation sequence, a polyhistidine tag, a maltose binding protein and a tobacco etch virus (TEV) protease cleavage site. These proteins were biotinylated in vivo at the N-terminal biotinylation sequence so that they could be applied to streptavidin coated ELISA plates.
Crude extracts from small-scale overnight protein expression experiments with the expression vector for the biotinylated fusion proteins were sufficient to saturate the ELISA plates based on prior optimization experiments. Dilutions of the antisera of interest were prepared in 2.5% skim milk in phosphate buffer saline (PBST) and applied to the plates for 1 hour at room temperature. After removal and washing the primary antibody was detected by HRP-conjugated goat anti-mouse, anti-rabbit or anti-swine IgG at a dilution of 1:100,000 (1:25,000 for anti-swine) for one hour at room temperature. The titre is expressed as the reciprocal of the last dilution with an A450>0.3 (greater than the mean plus three standard deviations of the background reading of wells without sera added).
The calculation of SEM error was done via ANOVA with Tukeys HSD (honest significant difference) test done as post hoc. The statistics showed that for all the sera, H49 is significantly different from H87 and that the N lobe is significantly different from the C lobe or the N+C lobe. The stars shown in
In this Example we demonstrate that three recombinant engineered C-lobes that represent a wide diversity of TbpBs present in the three porcine pathogens, may be linked together and retain antigenicity. The engineered C-lobes used here are those obtained from A. pleuropneumoniae, A. suis and Haemophilus parasuis (
Since there was no major barrier to the production of a stable C-lobe trimer, the C-lobe trimer was produced and purified for an immunization experiment to determine whether it retained immunogenicity. As illustrated in
In this Example, the loop regions of the N-lobe domain from three representative TbpBs from porcine pathogens were modified in order to determine their impact on induction of a cross-reactive immune response. The three representative TbpBs were from A. suis strain H57 (SEQ.ID NO: 28), A. pleuropneumoniae strain H87 (SEQ.ID NO: 12) and A. pleuropneumoniae strain H49 (SEQ.ID NO: 2). These particular TbpBs were selected because in addition to representing sequence diversity, there was high-resolution structural data available; A. suis strain H57 (3PQU.pdb), A. pleuropneumoniae strain H87 (3PQS.pdb) and A. pleuropneumoniae strain H49 (3HOL.pdb). The loop reduction process resulted in an overall loss of 74 amino acids (297-224) for the TbpB N-lobe from A. suis strain H57 (SEQ.ID NO: 36,38), 45 amino acids (248-203) for the TbpB N-lobe from A. pleuropneumoniae strain H87 (SEQ.ID NO: 24,26) and 27 amino acids (274-247) for the TbpB N-lobe from the A. pleuropneumoniae strain H49 (SEQ.ID NO: 8,10). The design of the loop reduction for A. pleuropneumoniae strain H49 is described in more detail below for illustrative purposes. In each case the first listed SEQ.ID. refers to the TbpB N-lobe sequence and the second SEQ.ID refers to the engineered loop reduction.
To design the loop reductions, the structure of TbpB from strain H49 (3HOL.pdb) was superimposed with the structure of TbpB from strain H87 (3PQS.pdb) while simultaneously viewing a multiple sequence alignment from a set of representative TbpBs from porcine pathogens. By examining the structure and regions of variation, several areas for potential loop reduction were identified that were predicted to not perturb the overall structure of the N-lobe. These include loops 1, 5, 8a, 8c and 12 according to the standard nomenclature (
A similar strategy was adopted to generate loop reductions for the TbpB N-lobes from A. pleuropneumoniae strain H87 and A. suis strain H57 since they represent considerable sequence and structural diversity of the TbpBs from porcine pathogens (
The resulting genes were cloned into a custom expression vector that encodes an N-terminal polyhistidine region, maltose binding protein and TEV (tobacco etch virus) cleavage site preceding the coding sequence for the cloned N-lobe. The expression plasmid for the engineered version of the TbpB N-lobe from A. pleuropneumoniae strain H49 was transformed into the E. coli expression strain ER256 that carries a chromosomal copy of the T7 RNA polymerase gene inserted downstream of the lacZ promoter. A small-scale expression analysis was performed using auto-induction media (55) that capitalizes on glucose repression and lactose induction of the lac promoter. Thus by using a specific ratio of glucose to lactose into the media, expression of the T7 RNA polymerase is optimally initiated at mid-log phase of growth. Cells were lysed after overnight growth with a bead beater to lyse the cells, the recombinant proteins were captured with either a Ni-NTA resin or a porcine Tf-Sepharose resin, washed and the bound proteins eluted in SD S-PAGE buffer.
As illustrated in
This example illustrates the use of engineered derivatives of TbpBs from the human pathogen Neisseria meningitidis in accordance with the present disclosure. As a first step we examined the diversity of TbpBs from N. meningitidis and ensured that we would have a representative set of TbpBs for our evaluation of cross-reactivity.
A phylogenetic tree illustrating the sequence diversity of the TbpB C-lobes (
In order to address the question as to whether a vaccine targeting the TbpB from N. meningitidis might induce an immune response directed against N. gonorrhoeae we performed an analysis of the sequence diversity of the TbpB and TbpB C-lobes from N. gonorrhoeae. The sequences of the representative TbpBs and TbpB C-lobes from the N. meningitidis study (
In order to compare the ability of the truncated TbpB and the TbpB C-lobe to induce a cross-reactive antibody response, a recombinant truncated TbpB and a TbpB C-lobe derived from N. meningitidis strain, B16B6 (marked by black arrow in
A representative set of TbpBs derived from strains distributed throughout the phylogenetic tree (black and grey arrows,
The proteins were expressed in our custom expression vector with an N-terminal biotinylation sequence, and were applied to streptavidin coated ELISA plates. The sera from rabbits immunized with the engineered C-lobe and truncated TbpB derived from strain B16B6 were tested for their ability to recognize the panel of TbpB variants. The antiserum against the truncated TbpB had higher titres against the homologous TbpB (B16B6) than the anti-C-lobe antiserum, and slightly higher or equivalent titres against the TbpB from one of the heterologous strains (H44/76). However, the anti-C-lobe antiserum had higher titres of antibody against TbpBs from all of the other heterologous strains than that anti-TbpB antiserum. Thus the C-lobe is superior in its ability to induce cross-reactive antibodies than the intact TbpB.
In this immunization experiment the recombinant antigens were produced as described above and used to immunize rabbits (New Zealand White, 3 months old, female) sub-cutaneously in the hind region using 50 μg of of purified antigen in 20% Emulsigen D (VSA) adjuvant. The rabbits were immunized at 0, 3 and 6 weeks.
Sera taken at week 8 were tested against representative proteins in our custom solid-phase ELISA assay. The recombinant fusion proteins used in the ELISA assay were produced as described above. The recombinant proteins tested in the custom ELISA assay were truncated versions of the intact TbpBs (missing the first 19-36 amino acids) from N. meningitidis strains; (i) B16B6 (SEQ.ID NO: 117), (ii) H44/76 (SEQ.ID NO: 133), (iii) 53131 (SEQ.ID NO:132), (iv) M990 (SEQ.ID NO:134), (v) M978 (SEQ.ID NO:135), (vi) M992 (SEQ.ID NO:138), (vii) P3006 (SEQ.ID NO:139), (viii) 120M (SEQ.ID NO:137), (ix) MC58 (SEQ.ID NO:136) and (x) M982 (SEQ.ID NO: 123).
Crude extracts from small-scale overnight protein expression experiments with the expression vector for the biotinylated fusion proteins were sufficient to saturate the ELISA plates based on prior optimization experiments. Dilutions of the antisera of interest were prepared in 2.5% skim milk in phosphate buffer saline (PBST) and applied to the plates for 1 hour at room temperature. The primary antibody was detected by HRP-conjugated goat anti-rabbit IgG at a dilution of 1:100,000 for one hour at room temperature and the titre is expressed as the reciprocal of the last dilution with an A450>0.3.
The result in
In this Example we demonstrate it is possible to substantially reduce the loop regions from the C-lobe of N. meningitidis. Such reduction is useful in that it provides a derivative lacking the large loop regions which may be used as a convenient ‘scaffold’ for displaying epitopes from other antigens or other pathogens, and further may display desirable immunological properties.
In this example the loop regions of the C-lobe domain from N. meningitidis strain M982 were modified to remove a total of 82 amino acids from the large, flexible loop regions. Structural models of the native C-lobe and the engineered C-lobe with substantial reduction of four of the large flexible loops are illustrated in Panel A of
The strategy for loop removal was developed by examination of the detailed structure (3VE2.pdb) while evaluating the sequence variability in sequence alignments of selected C-lobes. However, providing the most effective bridge between the loops without potentially disrupting the structure was a dominant criteria for the selection of bridging amino acids. The alignment of sequences of the native C-lobe (SEQ.ID NO: 125), engineered derivatives lacking each of the targeted loops and a ‘loopless’ C-lobe with all four loops removed (SEQ.ID NO: 129) is illustrated in Panel B of
Using the splicing by overlap extension approach (SOEing) (57) genes encoding the individual loop deletions and the ‘loopless’ C-lobe with all four loops removed were prepared. The SOEing approach was performed on the expression plasmid used for production of the recombinant C-lobe from N. meningitidis M982, so that the stability of the resulting engineered proteins could readily be evaluated. The vector encodes an N-terminal polyhistidine tag, the gene encoding maltose binding protein and a TEV (tobacco etch virus) cleavage site preceding the inserted gene encoding the TbpB C-lobe. The expression plasmid was transformed into the E. coli expression strain ER2566 that carries a chromosomal copy of the T7 RNA polymerase gene inserted into the lacZ gene, and thus is under the control of the lac promoter. A small-scale expression analysis was performed using auto-induction media (55). After overnight growth the cells were collected and lysed and the supernatant fraction after centrifugation was applied to a Ni-NTA resin, washed and the bound proteins eluted in SD S-PAGE buffer.
As illustrated in
Finally, as illustrated in
In these experiments FvB female mice were immunized with 25 ug of purified protein antigen with 20% emulisgen D on day 0, 21 and 42 and sera taken at day 56 were assessed for endpoint titre using our custom ELISA assay. The B16B6 TbpB C lobe, M982 TbpB C lobe, or modified M982 TbpB (‘loopless’) were used to immunized four mice each. Endpoint titres were assessed against biotinylated intact M982 or B16B6 TbpB protein. Titres were determined with 1:100,000 of goat anti-mouse IgG H+L peroxidase conjugated antibody. Endpoint titres were determined as the inverse of the last dilution at which a positive signal could be confidently detected. Each serum was run in triplicate and results are shown as the average of all mice with that treatment+/−SEM.
In this example, the DNA encoding segments of the extracellular surface loops of the integral outer membrane protein transferrin binding protein A, TbpA, were spliced into the gene encoding the modified or ‘loopless’ TbpB C-lobe from N. meningitidis strain M982, resulting in genes encoding various hybrid TbpA-TbpB proteins. The reason for splicing selected regions of TbpA onto the TbpB C-lobe scaffold is that it provides a more efficient means of production than the intact TbpA protein, and provides the ability to specifically target surface regions of TbpA for induction of antibody.
The gene encoding the modified C-lobe of the TbpB polypeptide (SEQ ID NO: 129) prepared in Example 6 was used as a starting point (see further: Panel A of
Assembly of the TbpA loops on the TbpB C-lobe was done using the splicing by overlap extension approach (SOEing) (57). The SOEing approach was performed on the expression plasmid used for production of the recombinant C-lobe from N. meningitidis M982, so that the stability of the resulting engineered proteins could readily be evaluated. The vector encodes an N-terminal polyhistidine tag, the gene encoding maltose binding protein and a TEV (tobacco etch virus) cleavage site preceding the inserted gene encoding the TbpB C-lobe. The expression plasmids were transformed into the E. coli expression strain ER2566 that carries a chromosomal copy of the T7 RNA polymerase gene inserted into the lacZ gene, and thus is under the control of the lac promoter. A small-scale expression analysis was performed using autoinduction media (55). After overnight growth the cells were collected and lysed and the supernatant fraction after centrifugation was applied to a Ni-NTA resin, washed and the bound proteins eluted in SD S-PAGE buffer.
As illustrated in Panel A of
Finally, as illustrated in
In this experiment, FvB female mice were immunized with 25 μg of purified protein antigen with 20% emulisgen D on day 0, 21 and 42 and sera taken at day 56 was assessed for endpoint titre using our custom ELISA assay. Three mice were immunized the ‘loopless’ C-lobe with all four loops removed (SEQ.ID NO: 97). Five mice were used for immunization with the each of the four other hybrid antigens; (i) the ‘loopless’ C-lobe with TbpA loop 10 inserted into the TbpB C-lobe loop 21(SEQ.ID NO: 154), (ii) the ‘loopless’ C-lobe with TbpA loop 11 inserted into the TbpB C-lobe loop 23 (SEQ.ID NO: 156), (iii) the ‘loopless’ C-lobe with TbpA loop 3 helix inserted into the TbpB C-lobe loop 27 (SEQ.ID NO: 158), or (iv) the ‘loopless’ C-lobe with TbpA plug loop inserted into the TbpB C-lobe loop 18 (SEQ.ID NO: 160). The sera were tested against the biotinylated recombinant form of the modified M982 C-lobe displaying all four of the TbpA loops inserted (SEQ.ID NO: 131). Titres were determined with 1:100,000 of goat anti-mouse IgG H+L peroxidase conjugated antibody. Endpoint titres were determined as the inverse of the last dilution at which a positive signal could be confidently detected. Each serum was run in triplicate and results are shown as the average of all mice with that treatment+/−SEM.
In this example, segments of the extracellular surface loops of the integral outer membrane protein lactoferrin binding protein A, LbpA, from strain MC58 (SEQ.ID NO: 162), were spliced into the modified TbpB C-lobe from N. meningitidis strain M982 (SEQ. ID NO: 129) that was described in Example 6. The reason for splicing selected regions of LbpA onto the TbpB C-lobe scaffold is that it provides a more efficient means of production than the intact LbpA protein, and provides the ability to specifically target surface regions of LbpA for induction of antibody. In conjunction with Example 7, we are able to illustrate how the generation of hybrid proteins could provide the opportunity to induce an immune response against three different proteins present on the surface of N. meningitidis, providing a greater barrier to potential ‘vaccine escape’, in which an antigenic variant of a critical target protein is able to escape the impact of the immune response generated against the vaccine antigens.
Since there were no structures available for LbpA, structural modeling of LbpA was performed using 3 web-based protein prediction servers: SWISS MODEL, I-TASSER, and PHYRE2 in an attempt to attain the most appropriate model. Initially BLAST searches were performed to find the most appropriate LbpA for modeling with the known TbpA structure (58), and revealed that the LbpA from strain MC58 (SEQ.ID NO: 130) was the most appropriate. An alignment of MC58 LbpA with K454 TbpA was generated by ClustalW and served as the input for the alignment mode in SWISS MODEL. In PHYRE2, the PDB ID for K454 TbpA structure and the FASTA sequence of MC58 LbpA were submitted as template and target, respectively. Only the FASTA sequence of MC58 LbpA was submitted to I-TASSER. The root-mean-square deviation (RMSD) was used to evaluate the similarity of the different models generated with the template structure after superimposing them in Pymol (http://www.pymol.org/). The LbpA model generated by PHYRE2 was selected as the most appropriate model and was used to select the loop regions for generating the hybrid or chimeric protein (
The ‘loopless’ C-lobe of the TbpB polypeptide prepared in Example 6 (SEQ.ID NO: 129) was used as a starting point. DNA encoding regions from the LbpA extracellular loop 3 and loop 2 were inserted between the DNA encoding the beta-strands flanking loops 18 and 21 of the engineered TbpB C-lobe (
The methods for transformation of the recombinant plasmid and performing preliminary expression trials are as described for Example 7. The preliminary screen demonstrated that the yields of recombinant protein were high (bottom left, Panel B,
The majority of the conjugate capsular vaccines that have been developed to date have used one of the toxin-based vaccine components as a carrier for conjugating the polysaccharide capsular material. There are several disadvantages to the strategy of conjugating the capsular polysaccharide to the tetanus or diphtheria toxin or toxoid. One is the potential for negatively influencing the induction of an effective immune response due to the continued exposure to the carrier proteins that are also present in vaccines used in routine immunizations; the development of immune tolerance. The second is that the carrier is not relevant to the natural exposure to the pathogen, thus will not fully take advantage of invoking the most relevant T-cell help when the pathogen is encountered.
Conjugate capsular vaccines have been very successful at preventing infection, and in fact preventing colonization, by the bacterial pathogens that express the specific capsular polysaccharide, normally referred to as the serotype or serogroup. However, there is essentially no cross-protection for bacteria expressing other polysaccharide capsular types, which can eventually lead to disease being caused by strains expressing polysaccharides not covered by the vaccine. The resulting need to expand the spectrum of polysaccharide capsular types covered by the conjugate capsular vaccines has led to the prospect of continual development of expanded spectrum vaccines and to the view that the ultimate solution lies with protein-based vaccines that are capable of providing substantial cross-protection. However, if a protein-based vaccine capable of inducing broad cross-protection was developed it is unlikely that it would be accepted to replace the existing conjugate capsular vaccines. The addition of yet another vaccine to the already crowed routine immunization schedule could be viewed as potential barrier to introduction of protein-based vaccines.
In this example we have engineered a conjugation loop into a TbpB C-lobe from H. influenzae that contains 42 lysine residues, substantially exceeding the total number of lysines in the remainder of the TbpB C-lobe, lysine residues, which would be predicted to minimize modification of lysines in critical epitopes since only those lysines reacting with the activated carbohydrate moieties would be modified and the ratio of carbohydrate to protein can be controlled during the conjugation process.
The sequence of the gene encoding the engineered H. influenzae TbpB C-lobe from strain H36 is illustrated Panel A of
The inclusion of a conjugation region would not be restricted to insertion into the loop regions of the N-lobe or C-lobe but for instance could be provided by including a cluster of lysine residues at the N-terminus of the intact TbpB or TbpB lobes.
A series of site-directed mutants in the surface loops of the TbpB protein were constructed to explore their impact on functional and immunological properties. In order to target surface exposed amino acids for modification, site-directed mutations were made in TbpBs for which we had the x-ray crystallography derived structures (12, 13). A splicing by overlap extension polymerase chain reaction (SOE PCR) approach was used to introduce mutations into genes encoding truncated TbpB proteins derived from the porcine pathogens A. pleuropneumoniae, A. suis and H. parasuis. They included A. pleuropneumoniae TbpB20-528 (amino acid 20-528) derived from strain H49 (ApH49 TbpB, SEQ.ID NO: 2), and a F171A mutant of ApH49 TbpB2° -528 (SEQ ID.NO: 4). Also included were A. pleuropneumoniae TbpB26-528 (amino acid 26-528) derived from strain H87 (ApH87 TbpB, SEQ.ID NO:12) and a Y95A mutant (SEQ.ID NO: 14), a Y121A mutant (SEQ.ID NO: 16), a Y174A mutant (SEQ.ID NO: 18) and a R179E mutant (SEQ.ID NO: 20). A. suis TbpB27-577 (amino acid 27-577) derived from strain H57 (AsH57 TbpB, SEQ.ID NO: 28), a F63A mutant (SEQ.ID NO: 30) and a F152A mutant (SEQ ID NO:32) were also produced. Finally, H. parasuis TbpB27-577 (amino acid 27-577) derived from the Nagasaki strain Hp5 (Hp5 TbpB, SEQ.ID NO: 115), a Y93A mutant (SEQ.ID NO: 170), a Y117A mutant (SEQ.ID NO: 172), a Y167A mutant (SEQ.ID NO: 174), and a W176A mutant (SEQ.ID NO: 176) were also prepared.
The recombinant proteins were initially produced with an N-terminal fusion partner containing a poly-histidine tag, a maltose binding protein and a TEV protease cleavage site. This enabled us to isolate recombinant protein suitable for a solid-phase binding assay using a nitrocellulose membrane and enzymatically labeled porcine transferrin or by capturing recombinant fusion protein from crude extracts with a porcine-Sepharose affinity resin (13). Using these assays, strong binding to by the native TbpBs was readily observed, whereas there was reduced binding by many of the site-directed mutants.
Although it is possible to derive semi-quantitative binding constants for binding of porcine Tf from these experiments we opted to utilize a variety of different biophysical and biochemical approaches to obtain more accurate and quantitative measures of the binding affinity. As shown in
Several of the mutations resulted in a ≥100 fold increase in the affinity constant (Kd), such as the F171A mutation in the TbpB from A. pleuropneumoniae strain H49, the Y174A mutation in the TbpB from A. pleuropneumoniae strain H87 or the Y167A and W176A double mutation- in the TbpB from H. parasuis strain HPS. It is interesting to note that these mutants all map to loop 8.
In order to test the immunological properties of the mutant proteins derived from TbpB that were defective in binding Tf it was important to test them in the native host, and preferably directly test their ability to protect the host from infection by the targeted pathogen. Thus experiments were initiated in a well-established infection model for Haemophilus parasuis in which colostrum-deprived piglets were immunized starting at 28 days after birth and challenged with Haemophilus parasuis at day 63 (37, 60). In this infection model a commercial vaccine derived from the challenge strain (Porcillis Glasser) provided complete protection from death and recombinant forms of transferrin receptors had previously provided 20-30% survival 15 days post-challenge.
Groups of five or six pigs were immunized with recombinant intact TbpB from the Hp5 challenge strain, the site-directed Y167A TbpB protein, the Porcillis Glasser vaccine or adjuvant alone. The pigs were challenged with the standard 108 challenge dose of H. parasuis Hp5 (Nagasaki) strain and monitored for a 15 day period. As illustrated in
To provide further evidence of the impact that the mutation had on the immune response the B-cell and T-cell responses were evaluated. Blood samples taken immediately prior to challenge (after two immunizations) and at 96 hours after challenge were analyzed for the adaptive immune response. The samples were evaluated by FACS analysis for mature B cell (αIgM+CD21+) and T helper cell (CD4+CD8α−) subsets. The results in
Although infection models do provide the opportunity to evaluate the potential efficacy of vaccines, they rarely emulate the natural infectious process, in which transmission of the pathogen normally leads to colonization of the host upper respiratory tract prior to establishment of infection. The conjugate capsular vaccines designed to prevent meningitis, pneumonia and invasive infection have been shown to eliminate the targeted bacteria from the upper respiratory tract (17), providing the additional advantage of herd immunity protecting non-immunized individuals. The ability to prevent colonization has since become an important feature for making decisions on vaccine implementation (18). Thus it may be prudent to design vaccines that prevent colonization so that along with preventing infection they can eliminate the reservoir of disease-causing pathogens.
Taking advantage of prior studies characterizing the interaction of N. meningitidis and human CEACAM receptors (61), we developed a transgenic humanized mouse model capable of supporting colonization by Neisseria meningitidis (62). This model is based on a specific interaction of Neisseria meningitidis Opa proteins with the human CEACAM1 receptor, and could potentially be extended to other pathogens that naturally or artificially exploit this interaction. Immunization of the transgenic mice with a meningococcal group C conjugate capsular vaccine resulted in sterilizing mucosal immunity in the colonization model, or in other words, prevented colonization by group C Neisseria meningitidis but not strains with other capsular types.
This model was used to test the ability of TbpB and its derivatives to prevent colonization by N. meningitidis. Since there is no human transferrin present in these mice during the immunization stage, it was not necessary to use an engineered non-binding TbpB as was described in Example 11. As illustrated in
In a follow up experiment we compared TbpB to another surface lipoprotein, factor H binding protein, which is a key component in two vaccines, and to the individual TbpB subdomains. As illustrated in
The colonization studies were performed as described previously (62). Groups of 8 or more C57/B16 expressing the human CEACAM-1 transgene (bred in-house) received 100 ul of designated immunizations subcutaneously on days 0 and 21. Groups received either the designated protein (25 ug) or no protein control adjuvanted with 20% Emulsigen D (MVP Laboratories) diluted in sterile phosphate buffered saline (PBS) (Gibco) to a volume of 100 ul per injection.
On day 35, mice were anesthetized with Isofluran (Baxter) and inoculated via intranasal instillation with the twice animal passaged N. meningitidis strain M982. To prepare inoculums, bacterial strains for infection were grown overnight on GC agar (Beckton Dickinson); the overnight lawn of growth was harvested into 1 ml of PBS containing 1 mM of MgCl2 (PBS/Mg) and OD600 was measured to adjust the number of bacteria. Cultures were adjusted such that each final 10 μl inoculum contained approximately 1×107 colony forming units. Density of colonization dose was confirmed via serial dilution plating on GC agar.
Three days after infection (day 38), mice were euthanized by carbon dioxide asphyxiation. Burden of colonization was assessed by tracheal lavage with 250 ul PBS/Mg followed by direct swabbing of the nasal passages with a polyester tipped applicator (Puritan Medical Products) resuspended in 500 ul PBS/Mg. Samples were enumerated after overnight growth on GC agar supplemented with VCNT inhibitor (Becton Dickinson) to prevent growth of nasal flora. Animal experiments were conducted in accordance with the Animal Ethics Review Committee of the University of Toronto.
Since the pathogens that possess TbpB reside exclusively in their specific host (humans, pigs, cattle and/or related ruminants) and since TbpB is capable of preventing colonization (
In order to broaden the efficacy of a vaccine formulation against a spectrum of Gram-negative pathogens, TbpBs, or portions thereof, e.g. a C-lobe domain, obtained from different bacterial species or strains may be combined. In this Example we provide preferred combinations of TbpB polypeptides or combinations thereof for use in the preparation of vaccine formulations.
One important consideration in identifying efficacious combinations of TbpB polypeptides is the extent to which different strains, species and genera are capable of readily exchanging the tbpB genes, thus acting as a potential reservoir for TbpB variants not covered by the vaccine. One of the important factors influencing the horizontal exchange of tbpB genes is the nature of the uptake signal sequence (USS) that is inherently present in these naturally transformable species (63, 64). These bacteria preferentially take up DNA containing the specific USS and incorporate it into their genome, providing a very efficient mechanism for incorporating antigenic variants of their surface antigens.
In the case of Neisseria meningitidis we have an extensive strain collection that adequately represented the overall sequence diversity (
The related pathogen, N. gonorrhoeae, that normally resides in the human genitourinary tract shares the same USS thus could potentially serve as a reserve for antigenic variation due to the occasional presence of these two species on the same mucosal surface. However, analysis of the sequence diversity of the gonococcal TbpBs relative to diversity in N. meningitidis (
The porcine pathogens A. pleuropneumoniae, A. suis and H. parasuis share the same USS and, as a consequence, the TbpB sequence diversity is distributed amongst the three species (
In regards to Haemophilus influenzae, the distinctly different spectrum of disease caused by the strains possessing the type b polysaccharide capsule and the non-typeable strains that lack a polysaccharide capsule have prompted focus on vaccines that target each group separately. Recent increases in invasive disease due to strains expressing the group A polysaccharide capsule has prompted consideration for development of vaccines targeting group A strains (65). An evaluation of TbpB diversity in strains of H. influenzae indicates that there are three major phylogenetic clusters (
Unlike Neisseria meningitidis and Haemophilus influenzae, there are no obvious USSs present in the genomes of Moraxella catarrhalis strains, yet they are naturally transformable with a strong preference for M. catarrhalis DNA. Thus development of a broadly cross-protective vaccine against M. catarrhalis with engineered antigens targeting TbpB need only consider the diversity of TbpBs from M. catarrhalis (
The bovine pathogen Mannhemia haemolytica, formerly known as Pasteurella haemolytica, is major cause of bovine respiratory disease (shipping fever) in cattle and respiratory infections in sheep. The sheep pathogen, Pasteurella trehalosi, that has been reclassified into two species Mannhemia glucosida and Bibersteinia trehalosi, shares USSs with Mannhemia haemolytica. This may be largely responsible for the finding that these species share a common gene pool (66). In contrast the bovine pathogen, Histophilus somni, formerly known as Haemophilus somnus, has a distinct USS, thus is not a reservoir of antigenic variants for M. haemolytica. There are three main phylogenetic lineages of TbpBs from M. haemolytica, M. glucosida and B. trehalosi that obviously encompass pathogens of sheep and cattle (66) (
All publications, patents and patent applications are herein incorporated by reference in their entirety to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety.
This application is a continuation of U.S. application Ser. No. 15/100,867 filed Jun. 1, 2016, which is a national phase entry of PCT/CA2014/051146 filed Dec. 1, 2014, which claims the benefit under 35 USC § 119(e) from U.S. Provisional Patent Application No. 61/910,817, filed on Dec. 2, 2013 and U.S. Provisional Patent Application No. 62/007,068, filed on Jun. 3, 2014, all of which are incorporated by reference herein in their entirety.
| Number | Date | Country | |
|---|---|---|---|
| 61910817 | Dec 2013 | US | |
| 62007068 | Jun 2014 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15100867 | Jun 2016 | US |
| Child | 16170420 | US |
