Claims
- 1. A method of inducing an immune response in an animal to H. influenzae, comprising administering an H. influenzae type b (Hib) polysaccharide-substantially pure, refolded meningococcal outer membrane protein (rPorB) conjugate to the animal in an amount effective to induce said immune response.
- 2. The method of claim 1, wherein said polysaccharide has a molecular weight range of 5,000 to 50,000.
- 3. The method of claim 1, wherein said conjugate is obtained by reductive amination of a Hib polysaccharide and rPorB, wherein the Hib polysaccharide has been oxidized or selectively hydrolyzed to give aldehyde groups.
- 4. The method of claim 1, wherein said conjugate is obtained by reductive amination of a Hib polysaccharide and rPorB, wherein the Hib polysaccharide has been oxidized to give aldehyde groups.
- 5. The method of claim 1, wherein said rPorB is a class 3 rPorB.
- 6. The method of claim 2, wherein said rPorB is a class 3 rPorB.
- 7. The method of claim 3, wherein said rPorB is a class 3 rPorB.
- 8. The method of claim 4, wherein said rPorB is a class 3 rPorB.
Parent Case Info
This application is a Divisional of application Ser. No. 09/118,180, filed Jul. 17, 1998, now U.S. Pat. No. 6,451,317, which claims priority under 35 U.S.C. 119(e) to U.S. Provisional Application No. 60/052,952, filed Jul. 17, 1997 and to U.S. Provisional Application No. 60/057,795, filed Sep. 8, 1997.
Non-Patent Literature Citations (3)
Entry |
Wolff et al. FEMS Microbiol. Letters. 1991. 83: 179-186.* |
Garner et al. Contrib. Microbiol. Immunol. 1989. 10:11-17.* |
Cruse et al. Illustrated Dictionary of Immunology. 1995. pp. 212 and 242. |
Provisional Applications (2)
|
Number |
Date |
Country |
|
60/052952 |
Jul 1997 |
US |
|
60/507795 |
Sep 1997 |
US |