Patent Grant
9758474
The present invention provides dihydroorotate dehydrogenase inhibitors, methods of preparing them, pharmaceutical compositions containing them and methods of treatment, prevention and/or amelioration of diseases or disorders wherein the inhibition of Dihydroorotate dehydrogenase is known to show beneficial effect.
In the recent past immense research has been dedicated to the discovery and understanding of the structure and functions of enzymes and bio-molecules associated with various diseases. One such important class of enzymes that has been the subject of extensive research is dihydroorotate dehydrogenase (DHODH).
DHODH is an enzyme that catalyzes the fourth step in the de novo biosynthesis of pyrimidine. It converts dihydroorotate (DHO) to orotate (ORO). Human DHODH is a ubiquitous flavine mononucleotide (FMN) moiety flavoprotein. In bacteria (gene pyrD), it is located on the inner side of the cytosolic membrane. In some yeasts, such as in Saccharomyces cerevisiae (gene URA1), it is a cytosolic protein while in other eukaryotes it is found in the mitochondria (see Proc. Natl. Acad. Sci. U.S.A., 89 (19), 8966-8970).
DHODH has been classified as a family of class I or class II proteins on the basis of the cofactor. Human DHODH belongs to the family class 2 that utilizes flavine as a redox cofactor, unlike the bacterial family class 1 protein that uses fumarate or NAD+ instead. In the cell the mammalian protein is anchored at the inner mitochondrial leaflet. There, DHODH catalyzes the conversion of DHO to ORO, which represents the rate limiting step in the de novo pyrimidine biosynthesis. (see McLean et al., Biochemistry 2001, 40, 2194-2200). Kinetic studies indicate a sequential ping-pong mechanism for the conversion of DHO to ORO (see Knecht et al., Chem. Biol. Interact. 2000, 124, 61-76). The first half-reaction comprises the reduction of DHO to ORO. Electrons are transferred to the FMN which becomes oxidized to dihydroflavin mononucleotide (FMNH2). After dissociation of ORO from the enzyme, FMNH2 is regenerated by a ubiquinone molecule, which is recruited from the inner mitochondrial membrane. Kinetic and structural studies revealed two distinct binding sites for DHO/ORO and ubiquinone, respectively.
Human DHODH is composed of two domains, a large C-terminal domain (Met78-Arg396) and a smaller N-terminal domain (Met30-Leu68), connected by an extended loop. The large C-terminal domain can be best described as an α/β-barrel fold with a central barrel of eight parallel β strands surrounded by eight α helices. The redox site, formed by the substrate binding pocket and the site that binds the cofactor FMN, is located on this large C-terminal domain. The small N-terminal domain, on the other hand, consists of two α helices (labeled α1 and α2), both connected by a short loop. This small N-terminal domain harbors the binding site for the cofactor ubiquinone. The helices α1 and α2 span a slot of about 10×20 Å2 in the so-called hydrophobic patch, with the short α1-α2 loop at the narrow end of that slot. The slot forms the entrance to a tunnel that ends at the FMN cavity nearby the α1-α2 loop. This tunnel narrows toward the proximal redox site and ends with several charged or polar side chains (Gln47, Tyr356, Thr360, and Arg136). Structural clues, as discussed above, along with kinetic studies suggest that ubiquinone, which can easily diffuse into the mitochondrial inner membrane, uses this tunnel to approach the FMN cofactor for the redox reaction (see Baumgartner et al., J. Med. Chem. 2006, 49, 1239-1247).
A study disclosed in The Journal of Biological Chemistry 2005, 280(23), 21847-21853; formally demonstrates the possibility to identify potent inhibitors of P. falciparum DHODH that do not inhibit the human enzyme. Comparison of the human DHODH crystal structures with the malaria DHODH amino acid sequence further suggests there are opportunities for species-specific inhibitor binding.
In the body, DHODH catalyzes the synthesis of pyrimidines, which are necessary for cell growth. An inhibition of DHODH inhibits the growth of (pathologically) fast proliferating cells, whereas cells which grow at normal speed may obtain their required pyrimidine bases from the normal metabolic cycle. The most important types of cells for the immune response, the lymphocytes, use exclusively the synthesis of pyrimidines for their growth and react particularly sensitively to DHODH inhibition.
DHODH inhibition results in decreased cellular levels of ribonucleotide uridine monophosphate (rUMP), thus arresting proliferating cells in the Gl phase of the cell cycle. The inhibition of de novo pyrimidine nucleotide synthesis is of great interest in view of the observations that lymphocytes seem not to be able to undergo clonal expansion when this pathway is blocked. Substances that inhibit the growth of lymphocytes are important medicaments for the treatment of auto-immune diseases.
During homeostatic proliferation, the salvage pathway which is independent of DHODH seems sufficient for the cellular supply with pyrimidine bases. Only, cells with a high turnover and particularly T and B lymphocytes need the de novo pathway to proliferate. In these cells, DHODH inhibition stops the cell cycle progression suppressing DNA synthesis and consequently cell proliferation (see Breedveld et al., Ann Rheum Dis 2000).
Therefore, inhibitors of DHODH show beneficial immunosuppressant and antiproliferative effects in human diseases characterized by abnormal and uncontrollable cell proliferation causing chronic inflammation and tissue destruction. The human enzyme dihydroorotate dehydrogenase (DHODH) represents a well-characterized target for small molecular weight Disease Modifying Antirheumatic Drugs (DMARDs).
A list of known DHODH inhibitors includes Leflunomide, Teriflunomide, Brequinar (NSC 368390) (Cancer Research 1992, 52, 3521-3527), Dichloroallyl lawsone (The Journal of Biological Chemistry 1986, 261(32), 14891-14895), Maritimus (FK 778) (Drugs of the Future 2002, 27(8), 733-739) and Redoxal (The Journal of Biological Chemistry 2002, 277(44), 41827-41834),
Leflunomide, teriflunomide, and brequinar have been studied significantly.
In general, inhibitors of DHODH show beneficial immunosuppressive and antiproliferative activities, most pronounced on T-cells (see Fairbanks et al., J. Biol. Chem. 1995, 270, 29682-29689). Brequinar and leflunomide are two examples of small molecular weight inhibitors of DHODH that had been in clinical development. The latter is used in the treatment of rheumatoid arthritis refractive to methotrexate (see Rozman J. Rheumatol Suppl. 1998, 53, 27-31; Pally et al., Toxicology 1998, 127, 207-222). Clinical application of both molecules suffers from various side effects. On the basis of very good efficacy in animal models, brequinar was originally developed for the therapy of organ transplant rejection but was switched to cancer as a secondary indication. The compound failed in the clinic due to its narrow therapeutic window. Oral administration of brequinar and some of its analogues resulted in toxic effects, including leukocytopenia and thrombocytopenia, when given in combination with cyclosporine. The application of leflunomide might be flawed by its long half-life time of approximately 2 weeks which represents a serious obstacle in patients that have developed side effects (see Fox et al. J. Rheumatol Suppl. 1998, 53, 20-26; Alldred et al., Expert Opin. Pharmacother. 2001, 2, 125-137).
In addition to abolish lymphocyte proliferation, inhibitors of DHODH (e.g., teriflunomide, maritimus (FK778) and brequinar) have an anti-inflammatory action by inhibition of cytokine production and nuclear factor (NF)-kB-signalling, monocyte migration and increased production of transforming growth factor beta-1 and induce a shift from T helper cell type 1 (TM) to type 2 (Th2) subpopulation differentiation (Manna et al., J. Immunol. 2000; Dimitrova et al., J. Immunol. 2002). Furthermore, the osteoclast differentiation mediated by Receptor Activator for Nuclear Factor k B Ligand (RANKL) decreased by DHODH inhibition (Urushibara et al., Arthrititis Rheum 2004). In co-crystallisation experiments with two inhibitors of DHODH that reached clinical trials, brequinar (Dexter et al., Cancer Res. 1985) and teriflunomide (A77-1726), were both found to bind in a common site, that is also believed to be the binding site of the cofactor ubiquinone (Liu et al., Struc. Fold. Des. 2000).
Leflunomide sold under the trade name Arava (EP 0 780 128, WO 97/34600), was the first DHODH inhibitor that reached the market place. Leflunomide is the prodrug of teriflunomide, which is the active metabolite inhibiting human DHODH with a moderate potency (Fox et al., J. Rheumatol. Suppl. 1998).
Leflunomide is a DMARD from Aventis, which was approved by the FDA for the treatment of rheumatoid arthritis in 1998 and by the EMEA for the treatment of psoriatic arthritis in 2004. Currently leflunomide is under active development for the treatment of systemic lupus erythematosus, Wegener's granulomatosis (Metzler et al., Rheumatology 2004, 43(3), 315-320) and HIV infection. Moreover, teriflunomide, its active metabolite is efficacious in multiple sclerosis and is currently in Phase III clinical trials (O'Connor et al., Neurology 2006).
Other data are emerging in other closely related diseases such as ankylosing spondilitis (Haibel et al., Ann. Rheum. Dis. 2005), polyarticular juvenile idiopathic arthritis (Silverman et al., Arthritis Rheum. 2005) and Sarcoidosis (Baughman et al., Sarcoidosis Vase. Diffuse Lung Dis. 2004). Furthermore, leflunomide and FK778 have shown antiviral activity against cytomegalovirus. Leflunomide is currently indicated as second-line therapy for cytomegalovirus disease after organ transplantation (John et al., Transplantation 2004). In addition leflunomide reduces HIV replication by about 75% at a concentration that can be obtained with conventional dosing (Schlapfer et al., AIDS 2003).
DHODH inhibitors under investigation at various stages of clinical trials are 4SC-101 (Phase-II) from 4SC AG; LAS-186323 (Phase-I) from Almirall Laboratories SA and ABR-224050, ABR-222417, & ABR-214658 (Preclinical) from Active Biotech AB. The exact structures of all these molecules have not yet been disclosed.
Various DHODH inhibitors have been disclosed for the treatment or prevention of autoimmune diseases, immune and inflammatory diseases, destructive bone disorders, malignant neoplastic diseases, angiogenic-related disorders, viral diseases, and infectious diseases. See for example WO2009137081; WO2009133379; WO 2009021696; WO2009082691; WO2009029473; WO2009153043; US2009209557; US2009 062318; US2009082374; WO2008097180; WO2008077639; US2008027079; US2007 299114; US2007027193; US2007224672; WO2007149211; JP2007015952; WO2006 044741; WO2006001961; WO2006051937; WO2006038606; WO2006022442; US2006 199856; WO2005075410; U.S. Pat. No. 7,074,831; WO2004056797; U.S. Pat. No. 7,247,736; WO2004056747; WO 2004056746; JP2004099586; WO2003097574; WO2003030905; WO2003006425; WO2003 006424; US2003203951; WO2002080897; U.S. Pat. No. 7,176,241; U.S. Pat. No. 7,423,057; WO2001024785; U.S. Pat. No. 6,841,561; WO9945926; WO9938846; WO9941239; EP767167 and U.S. Pat. No. 5,976,848.
For additional reviews and literature regarding DHODH inhibitors see Bio & Med. Chem. Letters, 20(6), 2010, Pages 1981-1984; J. Med. Chem. 2009, 52, 2683-2693; J. Med. Chem. 2008, 51 (12), 3649-3653. All of these patents, patent applications, and literature disclosures are incorporated herein as reference in their entirety for all purposes.
Despite the progress made in the area of DHODH inhibition in human diseases, challenges remain in terms of the side effects and desired clinical benefits from small molecule inhibitors. Accordingly, there still remains an unmet and dire need for small molecule DHODH inhibitors for the treatment and/or amelioration of diseases and disorders known to be associated with DHODH.
The present invention relates to compounds of formula (I), methods for their preparation, pharmaceutical compositions containing them and methods of use with them. The compound of formula (I) has the structure:
or a tautomer, stereoisomer (such as an enantiomer or diastereomer), pharmaceutically acceptable salt, pharmaceutically acceptable ester, prodrug or N-oxide thereof, wherein
Ring A is independently selected from a substituted or unsubstituted monocyclic aryl and a substituted or unsubstituted monocyclic heteroaryl, wherein each occurrence of X is independently CR4 or N;
Ring B is independently selected from a substituted or unsubstituted monocyclic aryl and a substituted or unsubstituted monocyclic heteroaryl, wherein each occurrence of X1 is independently CR4 or N;
R is hydrogen, substituted or unsubstituted (C1-6)alkyl or —ORa;
R1 is selected from —OH, —NRaOH, —COOH, —COORa, —CRaRbOH, —CRaRbCOOH, —SO2Ra, —CRaRbSO2Ra, —SO3Ra, —CRaRb—SO3Ra, —C(═Y)—NRaRb and —S(═O)q—NRaRb or an isostere of —COOH group, such as SO3H, CONHOH, B(OH)2, PO3RaRb, SO2NRaRb, a tetrazole, an amide, an ester or an acid anhydride or optionally represent halogen, substituted or unsubstituted (C1-6)alkyl or Cy1;
X2 is N or CR2 and X3 is N or CR3, wherein R2 and R3 may be same or different and are independently selected from hydrogen, halogen, substituted or unsubstituted (C1-6)alkyl or substituted or unsubstituted (C1-6)alkoxy.
L1 and L2 are independently absent or selected from —(CRaRb)n—, —O—, —S(═O)q—, —NRa—, —C(═Y)—, —C(═Y)—CRaRb, —CRaRb—C(═Y)—, —C(═Y)—C(═Y)—, —CRaRb—Y—, —C(═Y)—NRaRb—, —S(═O)q—NRaRb—, —NRaRb—C(═Y)—, —NRaRb—S(═O)q—, substituted or unsubstituted (C1-2)alkyl, substituted or unsubstituted (C2)alkenyl, and substituted or unsubstituted (C2)alkynyl; optionally each of substituted or unsubstituted (C1-2)alkyl, substituted or unsubstituted (C2)alkenyl, and substituted or unsubstituted (C2)alkynyl may be interrupted with —O—, —C(═Y)—, —S(═O)q— and —NRa—;
Cy is selected from substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocyclic group, substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl;
Cy1 is selected from substituted or unsubstituted monocyclic cycloalkyl, substituted or unsubstituted monocyclic heterocyclic group, substituted or unsubstituted monocyclic aryl and substituted or unsubstituted monocyclic heteroaryl;
R4 is independently selected hydrogen, hydroxy, halogen, cyano, —ORa, —S(═O)q—Ra, —NRaRb, —C(═Y)—Ra, —C(═Y)—ORa, —C(═Y)—NRaRb, —S(═O)q—NRaRb, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloalkylakyl, substituted or unsubstituted cycloalkenyl or when two R4 substituents are present, they may be joined to a form a substituted or unsubstituted saturated or unsaturated 3-10 member ring, which may optionally include heteroatoms which may be same or different and are selected from O, NRa or S, or alternatively when two R4 substituent are ortho to each other on an aromatic ring may be joined to form a substituted or unsubstituted saturated or unsaturated 4-10 member ring, which may optionally include one or more heteroatoms which may be same or different and are selected from O, NRa or S;
each occurrence of Ra and Rb may be the same or different and are independently selected from hydrogen, halogen, hydroxy, cyano, substituted or unsubstituted (C1-6)alkyl, —ORc (wherein Rc is substituted or unsubstituted (C1-6)alkyl) or when Ra and Rb are directly bound to a common atom, they may be joined to form an oxo group (═O) or form a substituted or unsubstituted saturated or unsaturated 3-10 member ring, which may optionally include heteroatoms which may be the same or different and are selected from O, NRa or S;
each occurrence of Y is independently selected from O, S and NRa;
each occurrence of n independently represents an integer 0, 1, 2, 3, or 4; and
each occurrence of q independently represents an integer 0, 1 or 2.
Some of the compounds of the present invention appear in different tautomeric forms. For example, Ring A can include a —C(OH)═N— group which interconverts to —C(O)—NH— and back to —C(OH)═N— again.
Yet another embodiment is a compound having the formula (I) wherein ring A is selected from
optionally substituted with one or more R4.
Yet another embodiment is a compound having the formula (I) wherein R1 is —COOH,
Yet another embodiment is a compound having the formula (I) wherein each occurrence of Y is O.
Yet another embodiment is a compound having the formula (I) wherein the variable Y between ring A and the group —N(R)— is O.
Yet another embodiment is a compound having the formula (I) wherein R is H.
Yet another embodiment is a compound having the formula (I) wherein each occurrence of X is CH, C—Cl, or C—F.
Yet another embodiment, is a compound having the formula (I) wherein each occurrence of X1 is CH, N or CF.
Yet another embodiment, is a compound having the formula (I) wherein X2 is CH, N, CCl or CF.
Yet another embodiment, is a compound having the formula (I) wherein X3 is CH, N, CCl or CF.
Yet another embodiment is a compound having the formula (I) wherein ring B is selected from
optionally substituted with one or more R4.
Yet another embodiment is a compound having the formula (I), wherein L1 and L2 are absent.
Yet another embodiment is a compound having the formula (I), wherein L1 is absent and L2 is —O—CRaRb.
Yet another embodiment, is a compound having the formula (I), wherein Cy is selected from
each optionally substituted with one or more R4.
In one preferred embodiment, Ring A is phenyl, Ring B is phenyl, Cy is phenyl or 3-ethyl-1H-indol-5-yl, R1 is —COOH, Y is O, L1 is absent, and L2 is absent. In a more preferred embodiment, Cy is phenyl substituted with one or more C1-C4 alkoxy, —S—(C1-C4 alkyl), and/or halogen. In a more preferred embodiment, Ring B is phenyl substituted with one or more fluoro groups.
Yet another embodiment is a compound of formula (IA)
or a tautomer, stereoisomer (such as an enantiomer or diastereomer), pharmaceutically acceptable salt, pharmaceutically acceptable ester, prodrug or N-oxide thereof, wherein
Ring A is selected from
optionally substituted with one or more R4, and R1 is independently selected from —OH, —NRaOH, —COOH, —COORa, or an isostere of —COOH group, such as SO3H, CONHOH, B(OH)2, PO3RaRb, SO2NHRa, a tetrazole, an amide, an ester or an acid anhydride.
Ring B is selected from
optionally substituted with one or more R4.
and all other variables are the same as described above in relation to formula (I).
Yet another embodiment is a compound of formula (IA)
or a tautomer, stereoisomer (such as an enantiomer or diastereomer), pharmaceutically acceptable salt, pharmaceutically acceptable ester, prodrug or N-oxide thereof, wherein
Ring A is selected from
Ring B is selected from
optionally substituted with one or more R4;
L2 is absent or is O—CRaRb—;
Cy is substituted phenyl, substituted indole or substituted indazole, such as
and all other variables are the same as described above in relation to formula (I).
Representative compounds of the present invention include those specified below and pharmaceutically acceptable salts thereof (Table 1). The present invention should not be construed to be limited to them.
Yet another embodiment is a method of inhibiting DHODH in a patient in need thereof by administering to the patient an effective amount of a compound of formula (I) or/and (IA).
Yet another embodiment is a method of inhibiting IL-17 in a patient in need thereof by administering to the patient an effective amount of a compound of formula (I) or/and (IA).
In particular compounds of formula (I) or/and (IA), or their pharmaceutically acceptable salts thereof are DHODH inhibitors useful in the treatment, prevention and/or amelioration of diseases or disorders wherein the inhibition of DHODH is known to show beneficial effect.
Another embodiment of the present invention is a method for treating an immunological disorder, inflammatory disorder, cancer or other proliferative disease via inhibition of DHODH by administering to a patient in need of such treatment an effective amount of at least one compound of formula (I) or/and (IA), as defined above.
Another embodiment of the present invention is a method for treating an immunological disorder, inflammatory disorder, cancer or other proliferative disease via inhibition of IL-17 either directly or by inhibition of DHODH by administering to a patient in need of such treatment an effective amount of at least one compound of formula (I) or/and (IA), as defined above.
Another embodiment of the present invention is a method for treating an immunological disorder, inflammatory disorder, cancer or other proliferative disease via inhibition of IL-17 as well DHODH by administering to a patient in need of such treatment an effective amount of at least one compound of formula (I) or/and (IA), as defined above.
Yet another embodiment of the present invention is a method for treating an immunological disorder, inflammatory disorder, cancer or other proliferative disease via inhibition of DHODH by administering to a patient in need of such treatment an effective amount of at least one compound of formula (I) or/and (IA), as defined above, in combination (simultaneously or sequentially) with at least one other anti-inflammatory, immunomodulator or anti-cancer agent.
The compounds of formula (I) or/and (IA), are useful in the treatment of a variety of disorders, including, but not limited to, autoimmune diseases, immune and inflammatory diseases, destructive bone disorders, cancers and malignant neoplastic diseases, angiogenic-related disorders, viral diseases, and infectious diseases. Such disorders include, but are not limited to:
The compounds of the present invention as modulators of apoptosis, are useful in the treatment of cancer (including but not limited to those types mentioned herein above), viral infections (including but not limited to herpevirus, poxvirus, Epstein-Barr virus, Sindbis virus and adenovirus), prevention of AIDS development in HIV-infected individuals, autoimmune diseases (including but not limited to systemic lupus, erythematosus, autoimmune mediated glomerulonephritis, rheumatoid arthritis, psoriasis, inflammatory bowel disease, and autoimmune diabetes mellitus), neurodegenerative disorders (including but not limited to Alzheimer's disease, AIDS-related dementia, Parkinson's disease, amyotrophic lateral sclerosis, retinitis pigmentosa, spinal muscular atrophy and cerebellar degeneration), myelodysplastic syndromes, aplastic anemia, ischemic injury associated with myocardial infarctions, stroke and reperfusion injury, arrhythmia, atherosclerosis, toxin-induced or alcohol related liver diseases, hematological diseases (including but not limited to chronic anemia and aplastic anemia), degenerative diseases of the musculoskeletal system (including but not limited to osteoporosis and arthritis) aspirin-sensitive rhinosinusitis, cystic fibrosis, multiple sclerosis, kidney diseases and cancer pain.
The compounds of present invention can modulate the level of cellular RNA and DNA synthesis. These agents are therefore useful in the treatment of viral infections (including but not limited to HIV, human papilloma virus, herpesvirus, poxvirus, Epstein-Barr virus, Sindbis virus and adenovirus).
The compounds of the present invention are useful in the chemoprevention of cancer. Chemoprevention is defined as inhibiting the development of invasive cancer by either blocking the initiating mutagenic event or by blocking the progression of pre-malignant cells that have already suffered an insult or inhibiting tumor relapse. The compounds are also useful in inhibiting tumor angiogenesis and metastasis.
The compounds of the present invention may also be combined with other active compounds in the treatment of diseases wherein the inhibition of DHODH is known to show beneficial effect.
In other embodiments, the diseases, conditions or disorders that benefit from inhibition of DHODH include, but are not limited to, an immune system-related disease (e.g., an autoimmune disease), a disease or disorder involving inflammation (e.g., asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, inflammatory bowel disease, glomerulonephritis, neuroinflammatory diseases, multiple sclerosis, uveitis and disorders of the immune system), cancer or other proliferative disease, hepatic diseases or disorders, renal diseases or disorders.
In one embodiment, compounds described herein are used as immunosuppresants to prevent transplant graft rejections, allogeneic or xenogeneic transplantation rejection (organ, bone marrow, stem cells, other cells and tissues), and graft-versus-host disease. In other embodiments, transplant graft rejections result from tissue or organ transplants. In further embodiments, graft-versus-host disease results from bone marrow or stem cell transplantation.
More particularly, the compounds of formula (I) or/and (IA) are useful in the treatment of a variety of inflammatory diseases including, but not limited to, inflammation, glomerulonephritis, uveitis, hepatic diseases or disorders, renal diseases or disorders, chronic obstructive pulmonary disease, rheumatoid arthritis, inflammatory bowel disease, vasculitis, dermatitis, osteoarthritis, inflammatory muscle disease, allergic rhinitis, vaginitis, interstitial cystitis, scleroderma, osteoporosis, eczema, allogeneic or xenogeneic transplantation, graft rejection, graft-versus-host disease, corneal transplant rejection, lupus erythematosus, systemic lupus erythematosus, prolipherative lupus nephritis, type I diabetes, pulmonary fibrosis, dermatomyositis, thyroiditis, myasthenia gravis, autoimmune hemolytic anemia, cystic fibrosis, chronic relapsing hepatitis, primary biliary cirrhosis, allergic conjunctivitis, hepatitis and atopic dermatitis, asthma and Sjogren's syndrome.
In one embodiment, the compounds described herein are useful in the treatment of a variety of diseases including Felty's syndrome, Wegener's granulomatosis, Crohn's disease, sarcoidosis, Still's disease, pemphigoid, Takayasu arteritis, systemic slerosis, relapsing polychondritis, refractory IgA nephropathy, SAPHO2 syndrome (SAS), cytomegalovirus infection including rhinitis or cyst, psoriasis and multiple myeloma.
The invention further provides pharmaceutical compositions comprising compounds having formula (I) or/and (IA), together with a pharmaceutically acceptable carrier.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood in the field to which the claimed subject matter belongs. In the event that there is a plurality of definitions for terms herein, those in this section prevail. Where reference is made to a URL or other such identifier or address, it is understood that such identifiers generally change and particular information on the internet comes and goes, but equivalent information is found by searching the internet. Reference thereto evidences the availability and public dissemination of such information.
It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of any subject matter claimed. In this application, the use of the singular includes the plural unless specifically stated otherwise. It must be noted that, as used in the specification and the appended claims, the singular forms “a,” “an” and “the” include plural referents unless the context clearly dictates otherwise. In this application, the use of “or” means “and/or” unless stated otherwise. Furthermore, use of the term “including” as well as other forms, such as “include”, “includes,” and “included,” is not limiting.
Definition of standard chemistry and molecular biology terms are found in reference works, including but not limited to, Carey and Sundberg “ADVANCED ORGANIC CHEMISTRY 4th edition” Vols. A (2000) and B (2001), Plenum Press, New York and “MOLECULAR BIOLOGY OF THE CELL 5th edition” (2007), Garland Science, New York. Unless otherwise indicated, conventional methods of mass spectroscopy, NMR, HPLC, protein chemistry, biochemistry, recombinant DNA techniques and pharmacology are contemplated within the scope of the embodiments disclosed herein.
Unless specific definitions are provided, the nomenclature employed in connection with, and the laboratory procedures and techniques of, analytical chemistry, and medicinal and pharmaceutical chemistry described herein are those generally used. In some embodiments, standard techniques are used for chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients. In other embodiments, standard techniques are used for recombinant DNA, oligonucleotide synthesis, and tissue culture and transformation (e.g., electroporation, lipofection). In certain embodiments, reactions and purification techniques are performed e.g., using kits of manufacturer's specifications or as described herein. The foregoing techniques and procedures are generally performed of conventional methods and as described in various general and more specific references that are cited and discussed throughout the present specification.
As used herein the following definitions shall apply unless otherwise indicated. Further many of the groups defined herein can be optionally substituted. The listing of substituents in the definition is exemplary and is not to be construed to limit the substituents defined elsewhere in the specification.
The term ‘alkyl’ refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing no unsaturation, having from one to eight carbon atoms, and which is attached to the rest of the molecule by a single bond, e.g., methyl, ethyl, n-propyl, 1-methylethyl (isopropyl), n-butyl, n-pentyl, and 1,1-dimethylethyl (t-butyl).
The term substituted or unsubstituted (C1-2)alkyl refers to an alkyl group as defined above having up to 2 carbon atoms, and the term substituted or unsubstituted (C1-6)alkyl refers to an alkyl group as defined above having up to 6 carbon atoms.
The term “alkenyl” refers to an aliphatic hydrocarbon group containing a carbon-carbon double bond which may be a straight or branched or branched chain having about 2 to about 10 carbon atoms, e.g., ethenyl, 1-propenyl, 2-propenyl (allyl), iso-propenyl, 2-methyl-1-propenyl, 1-butenyl, and 2-butenyl.
The term substituted or unsubstituted (C2)alkenyl refers to an alkenyl group as defined above having 2 carbon atoms.
The term “alkynyl” refers to a straight or branched chain hydrocarbyl radicals having at least one carbon-carbon triple bond, and having in the range of 2 up to 12 carbon atoms (with radicals having in the range of about 2 up to 10 carbon atoms presently being preferred), e.g., ethynyl, propynyl, and butnyl.
The term substituted or unsubstituted (C2) alkynyl refers to an alkynyl group as defined above having 2 carbon atoms.
The term “alkoxy” denotes an alkyl group as defined above attached via an oxygen linkage to the rest of the molecule. Representative examples of these groups are —OCH3 and —OC2H5.
The term “cycloalkyl” denotes a non-aromatic mono or multicyclic ring system of about 3 to 12 carbon atoms such as cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. Examples of multicyclic cycloalkyl groups include perhydronapththyl, adamantyl and norbornyl groups, bridged cyclic groups, and sprirobicyclic groups, e.g., sprio (4,4) non-2-yl.
The term “cycloalkylalkyl” refers to a cyclic ring-containing radical containing in the range of 3 up to 8 carbon atoms directly attached to an alkyl group which are then attached to the main structure at any carbon from the alkyl group that results in the creation of a stable structure such as cyclopropylmethyl, cyclobuyylethyl, and cyclopentylethyl.
The term “cycloalkenyl” refers to cyclic ring-containing radicals containing in the range of 3 up to 8 carbon atoms with at least one carbon-carbon double bond such as cyclopropenyl, cyclobutenyl, and cyclopentenyl. The term “cycloalkenylalkyl” refers to a cycloalkenyl group directly attached to an alkyl group which are then attached to the main structure at any carbon from the alkyl group that results in the creation of a stable structure
The term “aryl” refers to an aromatic radical having in the range of 6 up to 20 carbon atoms such as phenyl, naphthyl, tetrahydronapthyl, indanyl, and biphenyl.
The term “arylalkyl” refers to an aryl group as defined above directly bonded to an alkyl group as defined above. e.g., —CH2C6H5 and —C2H5C6H5.
The term “heterocyclic ring” refers to a non-aromatic 3 to 15 member ring radical which, consists of carbon atoms and at least one heteroatom selected from the group consisting of nitrogen, phosphorus, oxygen and sulfur. For purposes of this invention, the heterocyclic ring radical may be a mono-, bi-, tri- or tetracyclic ring system, which may include fused, bridged or spiro ring systems, and the nitrogen, phosphorus, carbon, oxygen or sulfur atoms in the heterocyclic ring radical may be optionally oxidized to various oxidation states. In addition, the nitrogen atom may be optionally quaternized. The heterocyclic ring radical may be attached to the main structure at any heteroatom or carbon atom that results in the creation of a stable structure.
The term “heteroaryl” refers to an optionally substituted 5-14 member aromatic ring having one or more heteroatoms selected from N, O, and S as ring atoms. The heteroaryl may be a mono-, bi- or tricyclic ring system. Examples of such heteroaryl ring radicals include, but are not limited to, oxazolyl, thiazolyl imidazolyl, pyrrolyl, furanyl, pyridinyl, pyrimidinyl, pyrazinyl, benzofuranyl, indolyl, benzothiazolyl, benzoxazolyl, carbazolyl, quinolyl and isoquinolyl. The heteroaryl ring radical may be attached to the main structure at any heteroatom or carbon atom that results in the creation of a stable structure.
Examples of such “heterocyclic ring” or “heteroaryl” radicals include, but are not limited to, azetidinyl, acridinyl, benzodioxolyl, benzodioxanyl, benzofurnyl, carbazolyl, cinnolinyl, dioxolanyl, indolizinyl, naphthyridinyl, perhydroazepinyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pyridyl, pteridinyl, purinyl, quinazolinyl, quinoxalinyl, quinolinyl, isoquinolinyl, tetrazoyl, imidazolyl, tetrahydroisouinolyl, piperidinyl, piperazinyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, 2-oxoazepinyl, azepinyl, pyrrolyl, 4-piperidonyl, pyrrolidinyl, pyrazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolinyl, oxasolidinyl, triazolyl, indanyl, isoxazolyl, isoxasolidinyl, morpholinyl, thiazolyl, thiazolinyl, thiazolidinyl, isothiazolyl, quinuclidinyl, isothiazolidinyl, indolyl, isoindolyl, indolinyl, isoindolinyl, octahydroindolyl, octahydroisoindolyl, quinolyl, isoquinolyl, decahydroisoquinolyl, benzimidazolyl, thiadiazolyl, benzopyranyl, benzothiazolyl, benzooxazolyl, furyl, tetrahydrofurtyl, tetrahydropyranyl, thienyl, benzothienyl, thiamorpholinyl, thiamorpholinyl sulfoxide thiamorpholinyl sulfone, dioxaphospholanyl oxadiazolyl, chromanyl, and isochromanyl.
The term “heteroarylalkyl” refers to a heteroaryl ring radical as defined above directly bonded to an alkyl group. The heteroarylalkyl radical may be attached to the main structure at any carbon atom from the alkyl group that results in the creation of a stable structure.
The term “heterocyclyl” refers to a heterocylic ring radical as defined above. The heterocylcyl ring radical may be attached to the main structure at any heteroatom or carbon atom that results in the creation of a stable structure.
The term “heterocyclylalkyl” refers to a heterocylic ring radical as defined above directly bonded to an alkyl group. The heterocyclylalkyl radical may be attached to the main structure at a carbon atom in the alkyl group that results in the creation of a stable structure.
The term “cyclic ring” refers to a cyclic ring containing 3-10 carbon atoms, optionally one or more of the ring carbon atoms may be replaced with heteroatom such as N, O, or S atom.
The term “monocyclic ring” refers to a single cyclic ring containing 3-10 carbon atoms, optionally one or more of the ring carbon atoms may be replaced with heteroatom such as N, O, or S atom.
The prefix “monocyclic ring” being used such as for example monocyclic aryl, refers to single aryl ring wherein the aryl is as defined herein above. Similarly the term monocyclic heteroaryl refers to a single heteroaryl ring wherein the heteroaryl is as defined herein above. The same is applicable to each of the terms monocyclic cycloalkyl and monocyclic heterocyclic ring as well.
The term “substituted” unless otherwise specified, refers to substitution with any one or any combination of the following substituents and may be the same or different which one or more are selected from hydrogen, hydroxy, halogen, carboxyl, cyano, nitro, oxo (═O), thio (═S), substituted or unsubstituted alkyl, substituted or unsubstituted alkoxy, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloalkenyl, substituted or unsubstituted cycloalkylalkyl, substituted or unsubstituted cycloalkenylalkyl, substituted or unsubstituted heterocyclic ring, substituted or unsubstituted heterocyclcyalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, substituted or unsubstituted guanidine, —COORx, —C(O)Rx, —C(S)Rx, —C(O)NRxRy, —C(O)ONRxRy, —NRyRz, —NRxCONRyRz, —N(Rx)SORy, —N(Rx)SO2Ry, —(═N—N(Rx)Ry), —NRxC(O)ORy, —NRxRy, —NRxC(O)Ry—, —NRxC(S)Ry—NRxC(S)NRyRz, —SONRxRy—, —SO2NRxRy—, —ORx, —ORxC(O)NRyRz, —ORxC(O)ORy—, —OC(O)Rx, —OC(O)NRxRy, —RxNRyC(O)Rz, —RxORy, —RxC(O)ORy, —RxC(O)NRyRz, —RxC(O)Ry, —RxOC(O)Ry, —SR, —SORx, —SO2Rx, —ONO2, wherein Rx, Ry and Rz in each of the above groups can be hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted alkoxy, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloalkenyl, substituted or unsubstituted cycloalkylalkyl, substituted or unsubstituted cycloalkenylalkyl, substituted or unsubstituted heterocyclic ring, substituted or unsubstituted heterocyclcyalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl, or any two of Rx, Ry and Rz may be joined to form a substituted or unsubstituted saturated or unsaturated 3-10 membered ring, which may optionally include heteroatoms which may be the same or different and are selected from O, NRX or S. Substitution or the combination of substituents envisioned by this invention are preferably those that result in the formation of a stable or chemically feasible compounds. The term stable as used herein refers to the compounds or the structure that are not substantially altered when subjected to conditions to allow for their production, detection and preferably their recovery, purification and incorporation into a pharmaceutical composition.
The term “halogen” or “halo” refers to radicals of fluorine, chlorine, bromine and iodine.
The term “protecting group” or “PG” refers to a substituent that is employed to block or protect a particular functionality. Other functional groups on the compound may remain reactive. For example, an “amino-protecting group” is a substituent attached to an amino group that blocks or protects the amino functionality in the compound. Suitable amino-protecting groups include, but are not limited to, acetyl, trifluoroacetyl, tert-butoxycarbonyl (BOC), benzyloxycarbonyl (CBz) and 9-fluorenylmethylenoxycarbonyl (Fmoc). Similarly, a “hydroxy-protecting group” refers to a substituent of a hydroxy group that blocks or protects the hydroxy functionality. Suitable hydroxy-protecting groups include, but are not limited to, acetyl and silyl. A “carboxy-protecting group” refers to a substituent of the carboxy group that blocks or protects the carboxy functionality. Suitable carboxy-protecting groups include, but are not limited to, —CH2CH2SO2Ph, cyanoethyl, 2-(trimethylsilyl)ethyl, 2-(trimethylsilyl)ethoxymethyl, -2-(p-toluenesulfonyl)ethyl, 2-(p-nitrophenylsulfenyl)ethyl, 2-(diphenylphosphino)-ethyl, and nitroethyl. For a general description of protecting groups and their use, see T. W. Greene, Protective Groups in Organic Synthesis, John Wiley & Sons, New York, 1991.
The term “stereoisomer” refers to compounds, which have identical chemical composition, but differ with regard to arrangement of the atoms and the groups in space. These include enantiomers, diastereomers, geometrical isomers, atropisomer or conformational isomers.
All the stereoisomers of compounds described herein are within the scope of this invention. Racemic mixtures are also encompassed within the scope of this invention. Therefore, single stereochemical isomers as well enantiomeric, diastereoisomeric and geometric (or conformational) mixtures of the present compounds fall within the scope of the invention.
The term “tautomers” refers to compounds, which are characterized by relatively easy interconversion of isomeric forms in equilibrium. These isomers are intended to be covered by this invention.
The term “prodrug” refers to a compound, which is an inactive precursor of a compound, converted into its active form in the body by normal metabolic processes.
The term “ester” refers to a compound, which is formed by reaction between an acid and an alcohol with elimination of water. An ester can be represented by the formula RCOOR′.
Additionally the instant invention also includes the compounds which differ only in the presence of one or more isotopically enriched atoms for example replacement of Hydrogen with Deuterium and the like.
Pharmaceutically acceptable salts forming part of this invention include salts derived from inorganic bases such as Li, Na, K, Ca, Mg, Fe, Cu, Zn, and Mn; salts of organic bases such as N,N′-diacetylethylenediamine, glucamine, triethylamine, choline, hydroxide, dicyclohexylamine, metformin, benzylamine, trialkylamine, thiamine, and the like; chiral bases like alkylphenylamine, glycinol, and phenyl glycinol, salts of natural amino acids such as glycine, alanine, valine, leucine, isoleucine, norleucine, tyrosine, cystine, cysteine, methionine, proline, hydroxy proline, histidine, ornithine, lysine, arginine, and serine; quaternary ammonium salts of the compounds of invention with alkyl halides, and alkyl sulphates such as MeI and (Me)2SO4, non-natural amino acids such as D-isomers or substituted amino acids; guanidine, substituted guanidine wherein the substituents are selected from nitro, amino, alkyl, alkenyl, alkynyl, ammonium or substituted ammonium salts and aluminum salts. Salts may include acid addition salts where appropriate which are, sulphates, nitrates, phosphates, perchlorates, borates, hydrohalides, acetates, tartrates, maleates, citrates, fumarates, succinates, palmoates, methanesulphonates, benzoates, salicylates, benzenesulfonates, ascorbates, glycerophosphates, and ketoglutarates. Pharmaceutically acceptable solvates may be hydrates or comprise other solvents of crystallization such as alcohols.
Additionally the instant invention also includes the compounds which differ only in the presence of one or more isotopically enriched atoms for example replacement of hydrogen with deuterium.
The term “subject” or “patient” encompasses mammals and non-mammals. Examples of mammals include, but are not limited to, any member of the Mammalian class: humans, non-human primates such as chimpanzees, and other apes and monkey species; farm animals such as cattle, horses, sheep, goats, and swine; domestic animals such as rabbits, dogs, and cats; and laboratory animals including rodents, such as rats, mice and guinea pigs. Examples of non-mammals include, but are not limited to, birds, fish and the like. In one embodiment of the methods and compositions provided herein, the mammal is a human.
The terms “treat,” “treating” or “treatment,” as used herein, include alleviating, abating or ameliorating a disease, disorder or condition symptoms, preventing additional symptoms, ameliorating or preventing the underlying causes of symptoms, inhibiting the disease, disorder or condition, e.g., arresting the development of the disease, disorder or condition, relieving the disease, disorder or condition, causing regression of the disease, disorder or condition, relieving a condition caused by the disease, disorder or condition, or stopping the symptoms of the disease, disorder or condition either prophylactically and/or therapeutically.
As used herein, “amelioration” refers to an improvement in a disease or condition or at least a partial relief of symptoms associated with a disease or condition and As used herein, amelioration of the symptoms of a particular disease, disorder or condition by administration of a particular compound or pharmaceutical composition refers to any lessening of severity, delay in onset, slowing of progression, or shortening of duration, whether permanent or temporary, lasting or transient that are attributed to or associated with administration of the compound or composition.
The terms “inhibits”, “inhibiting”, or “inhibitor” of DHODH, as used herein, refer to inhibition of the enzyme DHODH.
By “pharmaceutically acceptable,” as used herein, refers a material, such as a carrier or diluent, which does not abrogate the biological activity or properties of the compound, and is relatively nontoxic, i.e., the material is administered to an individual without causing undesirable biological effects or interacting in a deleterious manner with any of the components of the composition in which it is contained.
The term “pharmaceutical composition” refers to a mixture of a compound capable of inhibiting DHODH as described herein with other chemical components, such as carriers, stabilizers, diluents, dispersing agents, suspending agents, thickening agents, and/or excipients. The pharmaceutical composition facilitates administration of the compound to an organism. The compound and pharmaceutical composition of the present invention can be administered by various routes of administration including, but not limited to, intravenous, oral, aerosol, parenteral, ophthalmic, pulmonary and topical administration.
The terms “effective amount” or “therapeutically effective amount,” as used herein, refer to a sufficient amount of an agent or a compound being administered which will relieve to some extent one or more of the symptoms of the disease or condition being treated. The result is reduction and/or alleviation of the signs, symptoms, or causes of a disease, or any other desired alteration of a biological system. For example, an “effective amount” for therapeutic uses is the amount of the composition that includes a compound capable of inhibiting DHODH as described herein required to provide a clinically significant decrease in disease symptoms. In some embodiments, an appropriate “effective” amount in any individual case is determined using techniques, such as a dose escalation study.
The term “carrier,” as used herein, refers to relatively nontoxic chemical compounds or agents that facilitate the incorporation of a compound into cells or tissues.
The term “diluent” refers to chemical compounds that are used to dilute the compound of interest prior to delivery. In some embodiments, diluents are used to stabilize compounds because they provide a more stable environment. Salts dissolved in buffered solutions (which also provide pH control or maintenance) are utilized as diluents, including, but not limited to a phosphate buffered saline solution.
As used herein, the term “immune” include cells of the immune system and cells that perform a function or activity in an immune response, such as, but not limited to, T-cells, B-cells, lymphocytes, macrophages, dendritic cells, neutrophils, eosinophils, basophils, mast cells, plasma cells, white blood cells, antigen presenting cells and natural killer cells.
As used herein, “cytokine” or “cytokines” refers to small soluble proteins secreted by cells that in some embodiments, alter the behavior or properties of the secreting cell or another cell. Cytokines bind to cytokine receptors and trigger a behavior or property within the cell, for example, cell proliferation, death or differentiation. Exemplary cytokines include, but are not limited to, interleukins (e.g., IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, IL-18, IL-1α, IL-1β, and IL-1 RA), granulocyte colony stimulating factor (G-CSF), granulocyte-macrophage colony stimulating factor (GM-CSF), oncostatin M, erythropoietin, leukemia inhibitory factor (LIF), interferons, B7.1 (also known as CD80), B7.2 (also known as B70, CD86), TNF family members (TNF-α, TNF-β, LT-β, CD40 ligand, Fas ligand, CD27 ligand, CD30 ligand, 4-1BBL, Trail), and MIF.
The compounds of the present invention are also useful in combination (administered together or sequentially) with known immunomodulators and/or anti-inflammatory agents useful in the treatment of autoimmune diseases, immune and inflammatory diseases, destructive bone disorders, malignant neoplastic diseases, angiogenic-related disorders, viral diseases, and infectious diseases such as
The compounds of the invention are used for the treatment of rheumatoid arthritis, psoriatic arthritis, ankylosing spondilytis, multiple sclerosis, Wegener's granulomatosis, systemic lupus erythematosus, psoriasis and sarcoidosis it may be advantageous to use them in combination with other active compounds known to be useful in the treatment of such diseases such as rheumatoid arthritis, psoriatic arthritis, ankylosing spondilytis, multiple sclerosis, Wegener's granulomatosis, systemic lupus erythematosus, psoriasis and sarcoidosis.
The combinations of the invention may be used in the treatment of diseases and/or disorders wherein the inhibition of DHODH is known to show beneficial effect. Thus, the present application encompasses methods of treatment of these disorders, as well as the use of the combinations of the invention in the manufacture of a medicament for the treatment of these disorders.
The compounds of the present invention are also useful in combination (administered together or sequentially) with known anti-cancer treatments such as radiation therapy or with cytostatic or cytotoxic or anticancer agents, such as for example, but not limited to, DNA interactive agents, such as cisplatin or doxorubicin; topoisomerase II inhibitors, such as etoposide; topoisomerase I inhibitors such as CPT-11 or topotecan; tubulin interacting agents, such as paclitaxel, docetaxel or the epothilones (for example ixabepilone), either naturally occurring or synthetic; hormonal agents, such as tamoxifen; thymidilate synthase inhibitors, such as 5-fluorouracil; and anti-metabolites, such as methotrexate, other tyrosine kinase inhibitors such as Iressa and OSI-774; angiogenesis inhibitors; EGF inhibitors; VEGF inhibitors; CDK inhibitors; SRC inhibitors; c-Kit inhibitors; Her1/2 inhibitors and monoclonal antibodies directed against growth factor receptors such as erbitux (EGF) and herceptin (Her2) and other protein kinase modulators as well.
In some embodiments, diseases, disorders or conditions that are treated or prevented using compounds disclosed herein that are capable of inhibiting DHODH, compositions thereof, and methods provided herein to identify compounds capable of inhibiting DHODH, include diseases, conditions or disorders involving inflammation and/or that are related to the immune system. These diseases include but are not limited to asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, inflammatory bowel disease, glomerulonephritis, neuroinflammatory diseases such as multiple sclerosis, and disorders of the immune system.
Thus, in some embodiments, inhibition of DHODH results in a method for treating immune and immune-related disorders, including, for example, chronic immune diseases/disorders, acute immune diseases/disorders, autoimmune and immunodeficiency diseases/disorders, diseases/disorders involving inflammation, organ transplant graft rejections and graft-versus-host disease and altered (e.g., hyperactive) immune responses.
Examples of immune disorders include psoriasis, rheumatoid arthritis, vasculitis, inflammatory bowel disease, dermatitis, osteoarthritis, asthma, inflammatory muscle disease, allergic rhinitis, vaginitis, interstitial cystitis, scleroderma, osteoporosis, eczema, allogeneic or xenogeneic transplantation (organ, bone marrow, stem cells and other cells and tissues) graft rejection, graft-versus-host disease, lupus erythematosus, inflammatory disease, type I diabetes, pulmonary fibrosis, dermatomyositis, Sjogren's syndrome, thyroiditis (e.g., Hashimoto's and autoimmune thyroiditis), myasthenia gravis, autoimmune hemolytic anemia, multiple sclerosis, cystic fibrosis, chronic relapsing hepatitis, primary biliary cirrhosis, allergic conjunctivitis and atopic dermatitis.
In other embodiments, compounds disclosed herein that are capable of inhibiting DHODH, compositions thereof, and methods provided herein to identify compounds capable of modulating DHODH inhibitors, are used in connection with treatment of malignancies, including, but not limited to, malignancies of lymphoreticular origin, bladder cancer, breast cancer, colon cancer, endometrial cancer, head and neck cancer, lung cancer, melanoma, ovarian cancer, prostate cancer and rectal cancer. DHODH is thought to play an important role in cell proliferation in cancer cells.
The following general methodology described herein provides the manner and process of making and using the compound of the present invention and are illustrative rather than limiting. Further modification of provided methodology and additionally new methods may also be devised in order to achieve and serve the purpose of the invention. Accordingly, it should be understood that there may be other embodiments which fall within the spirit and scope of the invention as defined by the specification hereto.
General Method of Preparation of Compounds of the Invention
The compounds of the present invention may be prepared by the following processes. Unless otherwise indicated, the variables (e.g. A, B, Cy, R1, R, Y, L1, X, X1, X2, X3 and L2) when used in the below formulae are to be understood to present those groups described above in relation to formula (I) to (IA).
Scheme 1:
This scheme provides a method for the preparation of a compound of formula (I) wherein A is a substituted or unsubstituted monocyclic aryl and substituted or unsubstituted monocyclic heteroaryl and other variables such as B, Cy, R1, R, Y, L1, X, X1, X2, X3 and L2 are the same as described above in relation to formula (I).
The compound of formula (1) wherein Lg is a leaving group such as hydroxyl or halogen, can be coupled with a compound of formula (2) in a suitable solvent using an amide coupling reagent such as DCC or optionally in the presence of a suitable base to give an intermediate (for example R1 is COOEt) which can then be transformed either in single or multiple steps to the desired compound of formula (I), for example to R1 is COOH wherein A is a substituted or unsubstituted monocyclic aryl or a substituted or unsubstituted monocyclic heteroaryl and other variables such as L, B, Cy, R2, R3, R4, m and n are the same as described above in relation to formula (I).
Scheme 2:
This scheme provides a method for the preparation of a compound of formula (I) wherein R1 is COOH, L2 is absent, and other variables such as A, B, Cy, R1, R, Y, L1, X, X1, X2, and X3 are the same as described above in relation to formula (I).
The compound of formula (3), wherein Hal represents halogen, can be coupled with a compound of formula (4) in the presence of Pd(PPh3)4 and a metal carbonate such as K2CO3 (Suzuki coupling) to give a compound of formula (2a). The compound of formula (2a) can be reacted with a suitable compound of formula (Ia) in the presence of a suitable solvent to give the desired compound of formula (I) wherein R1 is COOH, L2 is absent and other variables such as A, B, Cy, R, Y, L1, X, X1, X2, and X3 are the same as described above in relation to formula (I).
Scheme 3:
This scheme provides a method for the preparation of a compound of formula (I) wherein R1 is COOH, L2 is —O—, and other variables such as A, B, Cy, R1, R, Y, L1, X, X1, X2, X3 and n are the same as described above in relation to formula (I).
The compound of formula (1a) can be reacted with a suitable compound of formula (2a) wherein Hal represents halogen in the presence of a suitable solvent to give the desired compound of formula (5). The compound of formula (5) can be coupled with a compound of formula (6) in the presence of a suitable base such as K2CO3 to give the desired compound of formula (I) wherein R1 is COOH, L2 is —O— and other variables such as A, B, Cy, R, Y, L1, X, X1, X2, and X3 are the same as described above in relation to formula (I).
Similar methodologies with certain modifications as known to those skilled in the art can be used to synthesize compound of formula (I) or/and (IA), wherein the variables are to be understood to present those groups described above in relation to formula (I) or/and (IA), using suitable intermediates and reagents.
Unless otherwise mentioned, work-up implies distribution of reaction mixture between the aqueous and organic phases indicated within parenthesis, separation and drying over Na2SO4 of the organic layer and evaporating the solvent to give a residue. Unless otherwise stated, purification is by column chromatography using silica gel as the stationary phase and a mixture of petroleum ether (boiling at 60-80° C.) and ethyl acetate or dichloromethane and methanol of suitable polarity as the mobile phases. RT refers to ambient temperature (25-28° C.).
General Procedure—for Suzuki Coupling:
To a solution of an aryl bromide (1 eq.) in dioxane and water (5:1) were added an arylboronic acid or an arylboronic acid pinacol ester (1.3 eq), tetrakis(triphenylphosphine)palladium(0)-(0.08 eq) and potassium carbonate (3.3 eq). The mixture was degassed with N2 for 30 min. and refluxed until both the staRTing materials disappeared as monitored by TLC. Work-up (H2O/AcOEt) and purification gave the desired product.
Intermediates 1, 4-27, 32-54, 56-63, 65-84 and 86-89 were prepared using general procedure-1.
The title compound (992 mg) was prepared from 2,6-difluoro-4-bromo aniline (1.1 g, 5.3 mmol) and 3-methoxyphenylboronic acid (1.04 g, 6.8 mmol) as a pale-yellow liquid. 1H-NMR (δ ppm, CDCl3+DMSO-d6, 400 MHz): 7.21-7.20 (m, 1H), 6.99-6.89 (m, 3H), 6.87-6.83 (m, 1H), 6.74-6.68 (m, 1H), 3.97 (bs, 2H), 3.69 (s, 3H).
The title compound (612 mg) was prepared from 2-chloro-5-nitropyridine (500 mg, 3.15 mmol) and 3-methoxyphenylboronic acid (623 mg, 4.1 mmol) as a pale yellow solid. 1H-NMR (δ ppm, CDCl3, 400 MHz): 9.50 (d, J 2.6, 1H), 8.53 (dd, J 2.6, 8.8, 1H), 7.91 (d, J 8.8, 1H), 7.69-7.68 (m, 1H), 7.63 (d, J 7.8, 1H), 7.44 (t, J 7.9, 1H), 7.08-7.05 (m, 1H), 3.91 (s, 3H).
Iron powder (739 mg, 13.24 mmol) and ammonium chloride (100 mg, 3.18 mmol) were added to a solution of intermediate 2 (610 mg, 2.65 mmol) in EtOH/H2O (2:1, 15 mL) and the mixture refluxed for one hour. The mixture was filtered through celite and celite washed with ethanol. Work-up (H2O/AcOEt) and concentration of the combined layers afforded intermediate 3 (330 mg) as a waxy solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 8.00 (d, J 2.7, 1H), 7.61 (d, J 8.5, 1H), 7.47-7.45 (m, 2H), 7.27 (t, J 8, 1H), 6.97 (dd, J 2.8, 8.5, 1H), 6.83-6.81 (m, 1H), 5.46 (s, 2H), 3.78 (s, 3H).
The title compound (1.34 g) was prepared from 2-fluoro-4-bromo aniline (2.5 g, 13.1 mmol) and 3-ethoxyphenylboronic acid (2.8 g, 17.0 T mmol) as a pale-yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.38-7.20 (m, 3H), 7.10 (d, J 7.9, 1H), 7.07-7.05 (m, 1H), 6.83-6.76 (m, 2H), 5.24 (s, 2H), 4.06 (q, J 7, 2H), 1.32 (t, J 7, 3H).
The title compound (0.219 g) was prepared from 2,6-difluoro-4-bromo aniline (0.5 g, 2.4 mmol) and 3-ethoxyphenylboronic acid (0.517 g, 3.12 mmol) as a pale-yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.30-7.24 (m, 3H), 7.14 (d, J 7.9, 1H), 7.11 (s, 1H), 6.82 (dd, J 2.1, 8.1, 1H), 5.31 (s, 2H), 4.07 (q, J 7, 2H), 1.32 (t, J 7, 3H).
The title compound (0.140 g) was prepared from 2,6-difluoro-4-bromo aniline (0.22 g, 1.06 mmol) and 2-chlorophenylboronic acid (0.21 g, 1.38 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.52-7.50 (m, 1H), 7.40-7.35 (m, 3H), 7.00 (d, J 2.1, 7.6, 2H), 5.39 (s, 2H).
The title compound (0.401 g) was prepared from 2,6-difluoro-4-bromo aniline (0.5 g, 2.4 mmol) and phenylboronic acid (0.38 g, 3.12 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.60 (d, J 7.4, 2H), 7.38 (t, J 7.5, 2H), 7.30-7.25 (m, 3H), 5.33 (s, 2H).
The title compound (0.812 g) was prepared from 2,6-difluoro-4-bromo aniline (1 g, 4.8 mmol) and 3-(trifluoromethoxy)phenylboronic acid (1.28 g, 6.24 mmol) as a colourless liquid.
The title compound (0.143 g) was prepared from 2,6-difluoro-4-bromo aniline (0.3 g, 1.4 mmol) and 3-(benzyloxy)phenylboronic acid (0.426 g, 1.8 mmol) as a colourless liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.49-7.44 (m, 2H), 7.39 (t, J 7.2, 2H), 7.35-7.26 (m, 4H), 7.24 (s, 1H), 7.18 (d, J 7.8, 1H), 6.91 (dd, J 1.9, 8.0, 1H), 5.33 (s, 2H), 5.16 (s, 2H).
The title compound (0.360 g) was prepared from 4-bromo-2-chloro-6-fluoroaniline (0.5 g, 2.0 mmol) and 3-ethoxyphenylboronic acid (0.441 g, 2.65 mmol) as a yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.43-7.38 (m, 2H), 7.27 (t, J 7.9, 1H), 7.14 (d, J 7.9, 1H), 7.10 (s, 1H), 6.82 (dd, J 2.1, 8.1, 1H), 5.51 (s, 2H), 4.07 (q, J 7, 2H), 1.32 (t, J 7, 3H).
The title compound (0.550 g) was prepared from 2,6-dichloro-4-bromoaniline (0.5 g, 2.0 mmol) and 3-methoxyphenylboronic acid (0.409 g, 2.69 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.58 (s, 2H), 7.29 (t, J 7.8, 1H), 7.18-7.10 (m, 2H), 6.85 (dd, J 2, 8.1, 1H), 5.63 (s, 2H), 3.81 (s, 3H).
The title compound (0.273 g) was prepared from 4-bromo-2-chloro-6-fluoroaniline (0.5 g, 2.2 mmol) and 3-propoxyphenylboronic acid (0.521 g, 2.9 mmol) as a yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.44-7.39 (m, 3H), 7.27 (t, J 7.9, 1H), 7.16-7.10 (m, 2H), 5.51 (s, 2H), 3.97 (t, J 6.5, 2H), 1.75-1.70 (m, 2H), 0.98 (t, J 7.9, 3H).
The title compound (0.392 g) was prepared from 4-bromo-2-chloro-6-fluoroaniline (0.5 g, 2.2 mmol) and 2-fluorophenylboronic acid (0.363 g, 2.6 mmol) as a pale-yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.51 (td, J 1.7, 7.8, 1H), 7.38-7.32 (m, 1H), 7.28 (s, 2H), 7.27-7.22 (m, 2H), 5.62 (s, 2H).
The title compound (0.550 g) was prepared from 4-Bromo-2,6-dichloroaniline (0.5 g, 2.2 mmol) and 3-ethoxyphenylboronic acid (0.447 g, 2.7 mmol) as a yellow liquid which was about 70% pure as adjudged by 1H-NMR data. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.56 (s, 2H), 7.28 (s, 1H), 7.16-7.08 (m, 2H), 6.83 (dd, J 2, 8.1, 1H), 5.62 (s, 2H), 4.10 (q, J 7, 2H), 1.32 (t, J 7, 3H).
The title compound (0.5 g) was prepared from 4-Bromo-2-fluoroaniline (1 g, 5.3 mmol) and 3-(trifluoromethoxy)phenylboronic acid (1.4 g, 6.8 mmol) as a yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.62 (d, J 8, 1H), 7.53 (s, 1H), 7.48 (t, J 8, 1H), 7.42 (dd, J 2, 9.1, 1H), 7.28 (dd, J 2, 8.3, 1H), 7.23 (d, J 11, 1H), 6.82 (t, J 8.5, 1H), 5.40 (s, 2H).
The title compound (0.294 g) was prepared from 2-trifluoromethoxy-4-bromo aniline (0.5 g, 1.9 mmol) and 2-fluorophenylboronic acid (0.345 g, 2.5 mmol) as a yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.47 (td, J 1.2, 4.7, 1H), 7.33-7.20 (m, 5H), 6.89 (d, J 8.8, 1H), 5.62 (s, 2H).
The title compound (0.367 g) was prepared from 4-Bromo-2,6-dichloro aniline (0.5 g, 1.9 mmol) and 2-fluorophenylboronic acid (0.363 g, 2.6 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.50 (t, J 6.3, 1H), 7.43 (s, 2H), 7.39-7.31 (m, 1H), 7.29-7.21 (m, 2H), 5.73 (s, 2H).
The title compound (0.34 g) was prepared from 4-Bromo-2,6-difluoro aniline (0.5 g, 2.4 mmol) and 3-isopropoxyphenylboronic acid (0.337 g, 3.1 mmol) as a red liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.30-7.22 (m, 3H), 7.16-7.07 (m, 2H), 6.89 (dd, J 2.3, 8.1, 1H), 5.32 (s, 2H), 4.68 (septet, J 6, 1H), 1.26 (d, J 6, 6H).
The title compound (0.3 g) was prepared from 4-Bromo-2,6-difluoro aniline (0.5 g, 2.4 mmol) and 3-propoxyphenylboronic acid (0.337 g, 3.1 mmol) as a red liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.30-7.24 (m, 3H), 7.18-7.10 (m, 2H), 6.86-6.80 (m, 1H), 5.31 (s, 2H), 3.97 (t, J 6.5, 2H), 1.75-1.65 (m, 2H), 0.98 (t, J 7.4, 3H).
The title compound (0.260 g) was prepared from 4-bromo-2-chloro-6-fluoroaniline (0.5 g, 2.4 mmol) and 2-chlorophenylboronic acid (0.453 g, 2.9 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.60 (d, J 7.3, 1H), 7.42-7.31 (m, 3H), 7.18-7.11 (m, 2H), 5.6 (s, 2H).
The title compound (0.190 g) was prepared from 4-bromo-2-chloro-6-fluoroaniline (0.2 g, 0.89 mmol) and 3-butoxyphenylboronic acid (0.224 g, 1.16 mmol) as a yellow solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.44-7.41 (m, 2H), 7.27 (t, J 7.9, 1H), 7.17-7.10 (m, 2H), 6.81-6.84 (m, 1H), 5.50 (s, 2H), 4.01 (t, J 5.3, 2H), 1.72-1.65 (m, 2H), 1.50-1.41 (m, 2H), 0.93 (t, J 7.4, 3H).
The title compound (0.180 g) was prepared from 4-bromo-2-chloro-6-fluoroaniline (0.2 g, 0.89 mmol) and 3-isobutoxyphenylboronic acid (0.224 g, 1.16 mmol) as a yellow solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.44-7.41 (m, 2H), 7.27 (t, J 7.9, 1H), 7.17-7.10 (m, 2H), 6.84-6.81 (m, 1H), 5.50 (s, 2H), 3.83 (d, J 6.5, 2H), 2.07-1.97 (m, 1H), 1.00 (d, J 6.7, 6H).
The title compound (0.492 g) was prepared from 4-bromo-2,6-difluoroaniline (0.5 g, 2.4 mmol) and 2-fluorophenylboronic acid (0.436 g, 3.12 mmol) as a yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.51 (td, J 1.5, 7.9, 1H), 7.38-7.31 (m, 1H), 7.29-721 (m, 2H), 7.14 (d, J 8.6, 2H), 5.44 (s, 2H).
The title compound (0.4 g) was prepared from 4-bromo-2,6-dichloroaniline (0.5 g, 2.1 mmol) and 2-chlorophenylboronic acid (0.421 g, 2.7 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.55-7.50 (m, 1H), 7.40-7.32 (m, 3H), 7.29 (s, 2H), 5.72 (s, 2H).
The title compound (0.16 g) was prepared from 4-bromo-2,6-difluoroaniline (0.2 g, 0.96 mmol) and 3-isobutoxyphenylboronic acid (0.242 g, 1.2 mmol) as a yellow liquid.
The title compound (0.104 g) was prepared from 4-bromo-2,6-difluoroaniline (0.2 g, 0.96 mmol) and 3-butoxyphenylboronic acid (0.242 g, 1.2 mmol) as a colourless liquid.
The title compound (0.280 g) was prepared from 4-bromo-2-chloro-6-difluoroaniline (0.5 g, 2.27 mmol) and 3-(trifluoromethoxy)phenylboronic acid (0.6 g, 2.89 mmol) as a pale-yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.68-7.64 (m, 1H), 7.62 (s, 1H), 7.53-7.46 (m, 3H) 7.29-7.22 (m, 1H), 5.64 (s, 2H).
Potassium carbonate (544 mg, 3.94 mmol) and benzyl alcohol (0.3 ml, 2.8 mmol) were added to a solution of 2,4,6-trifluoronitrobenzene (500 mg, 2.8 mmol) in DMF (5 ml). This mixture was stirred at RT overnight. Work up (EtOAc/H2O) afforded the title compound (521 mg) as a yellow liquid which was used in the next step without fuRTher purification.
Iron powder (502 mg, 9 mmol) and ammonium chloride (192 mg, 3.6 mmol) were added to a solution of intermediate 28 (500 mg, 1.8 mmol) in EtOH/H2O (2:1, 15 mL) and the mixture refluxed for two hours. The mixture was filtered through celite and celite washed with ethanol. Work-up (H2O/AcOEt) from the combined filtrates and purification of afforded the title compound (24 mg) as a colourless liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.42-7.36 (m, 3H), 7.35-7.28 (m, 2H), 6.68 (dd, J 1.6, 8.8, 2H), 4.97 (s, 2H), 4.64 (s, 2H).
Potassium carbonate (400 mg, 2.8 mmol) was added to 3-Bromophenol (500 mg, 2.8 mmol) dissolved in acetonitrile and the mixture was refluxed for 1 h and cooled to RT. cyclopentyl bromide (430 mg, 2.8 mmol) was added and the mixture refluxed again overnight. Work up (H2O/EtOAc) and purification afforded the title compound as a pale-yellow liquid. 1H-NMR (δ ppm, CDCl3, 400 MHz): 7.11 (t, J 8.3, 1H), 7.05-7.00 (m, 2H), 6.82-6.76 (m, 1H), 4.76-4.68 (m, 1H), 1.94-1.73 (m, 6H), 1.65-1.54 (m, 2H).
A mixture of intermediate 30 (335 mg, 1.4 mmol), bis(pinacolato)diboron (351 mg, 1.4 mmol) and potassium acetate (450 mg, 4.6 mmol) in dioxane was degassed with N2 for 30 min. Tetrakis(triphenylphosphine)palladium (0) (128 mg, 0.11 mmol) was added and the degassing continued again for fuRTher 15 min. This mixture was refluxed overnight. After completion of the reaction, work up (H2O/EtOAc) and column purification afforded the title compound (165 mg) as a yellow liquid.
The title compound (22 mg) was prepared from 4-bromo-2,6-difluoroaniline (165 mg, 0.6 mmol) and intermediate 31 (120 mg, 0.6 mmol) as a pale-yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.29-7.22 (m, 3H), 7.12 (d, J 8, 1H), 7.06 (s, 1H), 6.82-6.76 (m, 1H), 5.30 (s, 2H), 4.96-4.88 (m, 1H), 1.96-1.82 (m, 2H), 1.75-1.65 (m, 4H), 1.60-1.51 (m, 2H).
The title compound (450 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (600 mg, 2.7 mmol) and intermediate 31 (1 g, 3.4 mmol) as a yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.42-7.36 (m, 2H), 7.26 (t, J 8, 1H), 7.11 (d, J 7.8, 1H), 7.08-7.05 (m, 1H), 6.80 (dd, J 2.3, 8.1, 1H), 5.49 (s, 2H), 4.92-4.84 (m, 1H), 1.97-1.87 (m, 2H), 1.77-1.62 (m, 4H), 1.60-1.50 (m, 2H).
The title compound (144 mg) was prepared from 4-bromo-2,6-difluoroaniline (200 mg, 0.96 mmol) and 3-(difluoromethoxy)phenylboronic acid (234 mg, 1.25 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.49 (d, J 7.9, 1H), 7.43-7.39 (m, 2H), 7.38-7.30 (m, 2H), 7.32 (t, J 74, 1H), 7.06 (dd, J 1.8, 7.9, 1H), 5.42 (s, 2H).
The title compound (155 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (89 mg, 1.16 mmol) and 3-(difluoromethoxy)phenylboronic acid (217 mg, 1.16 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.52-7.47 (m, 3H), 7.46-7.44 (m, 1H), 7.43-7.40 (m, 2H), 7.32 (t, J 74.2, 1H), 5.60 (s, 2H).
The title compound (97 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 2-chloro-5-methoxyphenylboronic acid (116 mg, 0.62 mmol) as a yellow solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.40 (d, J 9.6, 1H), 7.02 (d, J 7.7, 2H), 6.92 (s, 2H), 5.39 (s, 2H), 3.77 (s, 3H).
The title compound (51 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 3-fluoro-5-methoxyphenylboronic acid (106 mg, 0.62 mmol) as a yellow solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.35 (dd, J 2.1, 8.1, 2H), 7.06 (d, J 10.2, 1H), 7.01 (s, 1H), 6.71 (dd, J 2, 8.8, 1H), 5.42 (s, 2H), 3.81 (s, 3H).
The title compound (143 mg) was prepared from 4-bromo-2,6-difluoroaniline (200 mg, 0.96 mmol) and benzo[d][1,3]dioxol-5-ylboronic acid (207 mg, 1.25 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.23-7.18 (m, 3H), 7.08 (dd, J 1.8, 8.2, 1H), 6.91 (d, J 8.1, 1H), 6.01 (s, 2H), 5.24 (s, 2H).
The title compound (143 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (200 mg, 0.89 mmol) and benzo[d][1,3]dioxol-5-ylboronic acid (192 mg, 1.16 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.36-7.30 (m, 2H), 7.20 (d, J 1.7, 1H), 7.06 (dd, J 1.4, 8.1, 1H), 6.91 (d, J 8.1, 1H), 6.01 (s, 2H), 5.42 (s, 2H).
The title compound (39 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 3,4-dimethoxyphenylboronic acid (113 mg, 0.62 mmol) as a pale-yellow solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.25 (dd, J 2, 8.3, 2H), 7.18-7.10 (m, 2H), 6.94 (d, J 8.4, 1H), 5.21 (s, 2H), 3.81 (s, 3H), 3.75 (s, 3H).
The title compound (71 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (100 mg, 0.44 mmol) and 3-fluoro-5-methoxyphenylboronic acid (97 mg, 0.57 mmol) as a pale-yellow solid. 1H-NMR (8 ppm, DMSO-d6, 400 MHz): 7.51-7.45 (m, 2H), 7.05 (d, J 10.3, 1H), 7.00 (s, 1H), 6.73 (dd, J 2.1, 4.2, 1H), 5.60 (s, 2H), 3.81 (s, 3H).
The title compound (41 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (100 mg, 0.44 mmol) and 3-chloro-5-methoxyphenylboronic acid (107 mg, 0.57 mmol) as a pale-yellow solid.
The title compound (182 mg) was prepared from 4-bromo-2,6-difluoroaniline (200 mg, 0.96 mmol) and 2,3-dihydrobenzofuran-5-ylboronic acid (204 mg, 1.25 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.46 (s, 1H), 7.30 (dd, J 1.8, 8.3, 1H), 7.16 (dd, J 2.1, 8.2, 2H), 6.75 (d, J 8.3, 1H), 5.18 (s, 2H), 4.52 (t, J 8.7, 2H), 3.18 (t, J 8.5, 2H).
The title compound (86 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (200 mg, 0.89 mmol) and 2,3-dihydrobenzofuran-5-ylboronic acid (189 mg, 1.16 mmol) as a pale-yellow solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.46 (s, 1H), 7.33-7.25 (m, 3H), 6.75 (d, J 8.3, 1H), 5.36 (s, 2H), 4.52 (t, J 8.7, 2H), 3.18 (t, J 8.6, 2H).
Potassium acetate (0.344 g, 3.51 mmol) and bis(pinacolato)diboron (351 mg, 1.4 mmol) were added to a solution of 5-bromo-1,3-dimethyl-1H-indazole (240 mg, 1.06 mmol) in dioxane (10 ml) and mixture was degassed with nitrogen for 30 min. tetrakis(triphenylphosphine) palladium(0) was added and degassed for further 30 min. Reaction mixture was refluxed for 2 h. After completion of the reaction, work-up (AcOEt/H2O) followed by column afforded 1,3-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indazole (85 mg) as a white solid. The title compound (26 mg) was prepared from 4-bromo-2,6-difluoroaniline (50 mg, 0.24 mmol) and 1,3-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indazole (85 mg, 0.31 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.93 (s, 1H), 7.66 (dd, J 1.5, 8.8, 1H), 7.55 (d, J 8.8, 1H), 7.33 (dd, J 2, 8.2, 2H), 5.22 (s, 2H), 3.94 (s, 3H), 2.49 (s, 3H).
The title compound (39 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 3-chloro-5-methoxyphenylboronic acid (116 mg, 0.62 mmol) as a pale-yellow solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.36 (dd, J 2.2, 8.1, 2H), 7.25 (s, 1H), 7.12 (s, 1H), 6.91 (t, J 1.9, 1H), 5.43 (s, 2H), 3.81 (s, 3H).
The title compound (39 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (100 mg, 0.442 mmol) and 3,4-dimethoxyphenylboronic acid (80 mg, 0.62 mmol) as a pale-yellow solid.
The title compound (39 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (100 mg, 0.44 mmol) and 2-chloro-5-methoxyphenylboronic acid (106 mg, 0.62 mmol) as a pale-yellow solid.
The title compound (31 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 2-fluoro-5-methoxyphenylboronic acid (106 mg, 0.62 mmol) as a pale-yellow solid.
The title compound (81 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 4-chloro-5-methoxyphenylboronic acid (116 mg, 0.62 mmol) as a white solid.
The title compound (82 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (100 mg, 0.44 mmol) and 4-chloro-5-methoxyphenylboronic acid (107 mg, 0.62 mmol) as a pale-yellow solid.
The title compound (70 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (100 mg, 0.44 mmol) and 2-fluoro-5-methoxyphenylboronic acid (98 mg, 0.58 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.30-7.24 (m, 2H), 7.17 (t, J 9.1, 1H), 7.02-6.97 (m, 1H), 6.90-6.84 (m, 1H), 5.61 (s, 2H), 3.77 (s, 3H).
The title compound (480 mg) was prepared from 4-bromo-2,6-difluoroaniline (200 mg, 0.96 mmol) and 4-fluoro-5-methoxyphenylboronic acid (210 mg, 1.24 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.39-7.29 (m, 3H), 7.21-7.12 (m, 2H), 5.33 (s, 2H), 3.90 (s, 3H).
The title compound (68 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 3-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indole (160 mg, 0.62 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 10.71 (s, 1H), 7.67 (s, 1H), 7.33-7.29 (m, 2H), 7.28-7.21 (m, 2H), 7.09 (s, 1H), 5.12 (s, 2H), 2.27 (s, 3H).
Following the general procedure-1, tert-butyl 5-(4-amino-3,5-difluorophenyl)-3-methyl-1H-indazole-1-carboxylate (106 mg) was prepared from 4-bromo-2,6-difluoroaniline (250 mg, 1.2 mmol) and teRT-butyl 3-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indazole-1-carboxylate (559 mg, 1.56 mmol) as a white solid. teRT-butyl 5-(4-amino-3,5-difluorophenyl)-3-methyl-1H-indazole-1-carboxylate (205 mg) was dissolved in dichloromethane (4 ml), trifluoroacetic acid (0.8 ml) was added and stirred at RT for 4 h. The solvent was removed and the residue co-evaporated 4 times with dihloromethane. Solid obtained was dried under high vacuum to obtain the title compound (243 mg) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 9.01 (bs, 1H), 7.92 (s, 1H), 7.58 (dd, J 1.4, 8.7, 1H), 7.44 (d, J 8.7, 1H), 7.32 (dd, J 2, 8.2, 2H), 5.22 (s, 2H), 2.50 (s, 3H).
The title compound (83 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 3-ethylphenylboronic acid (86 mg, 0.57 mmol) as a yellow liquid.
The title compound (65 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (120 mg, 0.53 mmol) and 3-ethoxy-2-fluorophenylboronic acid (120 mg, 0.69 mmol) as a colourless liquid.
The title compound (68 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 2,3-dihydrobenzo[b][1,4]dioxin-6-ylboronic acid (160 mg, 0.62 mmol) as a white solid.
The title compound (85 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (100 mg, 0.44 mmol) and 3-(2,2,2-trifluoroethoxy)phenylboronic acid (127 mg, 0.57 mmol) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.53-7.44 (m, 2H), 7.38-7.26 (m, 3H), 6.94 (d, J 7, 1H), 5.55 (s, 2H), 4.83 (q, J 8.9, 2H).
The title compound (430 mg) was prepared from 4-bromo-6-fluoroaniline (500 mg, 2.63 mmol) and 3-Methoxyphenylboronic acid (519 mg, 3.42 mmol) as a red liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.34 (dd, J 2, 13.1, 1H), 7.27 (t, J 7.9, 1H), 7.22 (dd, J 2, 8.3, 1H), 7.12 (d, J 7.9, 1H), 7.09-7.07 (m, 1H), 6.84-6.78 (m, 2H), 5.29 (s, 2H), 3.78 (s, 3H).
The title compound (200 mg) was prepared from 4-bromoaniline (300 mg, 1.74 mmol) and 3-ethoxyphenylboronic acid (380 mg, 2.29 mmol) as a colourless liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 7.33 (d, J 8.5, 2H), 7.26-7.22 (m, 1H), 7.06 (d, J 7.9, 1H), 7.01 (s, 1H), 6.75 (dd, J 5.8, 8.1, 1H), 6.60 (d, J 8.5, 2H), 5.20 (s, 2H), 4.04 (q, J 7, 2H), 1.16 (t, J 7, 3H).
The title compound (900 mg) was prepared from 4-bromo-2,6-difluoroaniline (1 g, 4.8 mmol) and 3-(ethylthio)phenylboronic acid (1.13 g, 6.24 mmol) as a colourless liquid.
The title compound (170 mg) was prepared from 4-bromo-2,6-difluoroaniline (1.2 g, 5.76 mmol) and 2-(3-cyclopropoxyphenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (1.5 g, 5.76 mmol) as a yellow liquid.
5-Bromoisatin (5 g, 22.12 mmol) was added drop-wise to ethyl magnesium iodide formed from ethyl iodide (14 ml, 176.96 mmol) and magnesium (8.6 g, 176.96 mmol) in Et2O (50 ml) and stirred at RT overnight. Work-up (EtOAc/H2O) after adding aq. 10% NH4Cl solution and purification gave 5-bromo-3-ethyl-3-hydroxyindolin-2-one (1 g). To a solution of this intermediate (1 g, 3.93 mmol) in THF (40 ml) was added a 2M solution borane-dimethyl sulphide in THF (10 ml, 19.65 mmol) and stirred at RT for 3 h. Work up (EtOAc/H2O) and purification using afforded 5-Bromo-3-ethyl indole (800 mg). To a solution of 5-Bromo-3-ethyl indole (800 mg, 3.57 mmol) in dioxane (10 ml) were added bis(pinacolato)diboron (1.078 g, 4.64 mmol), potassium acetate (1.15 g, 11.78 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]dichloro palladium(II).CH2Cl2, degassed for 30 min. and refluxed overnight. Work-up followed by purification afforded title compound (800 mg) as an off-white solid.
The title compound (220 mg) was prepared from 4-bromo-2,6-difluoroaniline (662 mg, 2.57 mmol) and intermediate 64 (440 mg, 1.78 mmol) as a yellow liquid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 10.74 (s, 1H), 7.69 (s, 1H), 7.33-7.29 (m, 2H), 7.23 (dd, J 1.8, 8.3, 2H), 7.09 (s, 1H), 5.12 (s, 2H), 2.73 (q, J 7.5, 2H), 1.27 (q, J 7.5, 3H).
The title compound (280 mg) was prepared from 4-bromo-2,3,5,6-tetrafluoroaniline (300 mg, 1.22 mmol) and 3-(ethylthio)phenylboronic acid (291 mg, 1.6 mmol) as a colourless liquid.
The title compound (280 mg) was prepared from 4-bromo-3-fluoroaniline (227 mg, 1.2 mmol) and 2-chloro-5-methoxyphenylboronic acid (290 mg, 1.6 mmol) as a pale-yellow liquid.
The title compound (280 mg) was prepared from 4-bromo-2-fluoroaniline (390 mg, 1.6 mmol) and 3-propoxyphenylboronic acid (369 mg, 2 mmol) as a colourless liquid.
The title compound (280 mg) was prepared from 4-bromoaniline (300 mg, 1.7 mmol) and 3-propoxyphenylboronic acid (408 mg, 2.3 mmol) as a colourless liquid.
The title compound (430 mg) was prepared from 4-bromo-3-fluoroaniline (300 mg, 1.6 mmol) and 3-(ethylthio)phenylboronic acid (370 mg, 2.0 mmol) as a yellow liquid.
The title compound (89 mg) was prepared from 4-bromo-2,6-difluoroaniline (80 mg, 0.38 mmol) and 3-(2,2,2-trifluoroethoxy)phenylboronic acid (108 mg, 0.5 mmol) as a colourless liquid.
The title compound (89 mg) was prepared from 4-bromo-2,6-difluoroaniline (100 mg, 0.48 mmol) and 3-ethylphenylboronic acid (99 mg, 0.6 mmol) as a colourless liquid.
The title compound (176 mg) was prepared from 4-bromoaniline (300 mg, 1.74 mmol) and 2-chlorophenylboronic acid (354 mg, 2.26 mmol) as a yellow solid.
The title compound (58 mg) was prepared from 4-bromoaniline (300 mg, 1.74 mmol) and 3-methoxyphenylboronic acid (344 mg, 2.26 mmol) as a yellow liquid.
The title compound (280 mg) was prepared from 4-bromoaniline (300 mg, 1.74 mmol) and 3-(trifluoromethoxy)phenylboronic acid (466 mg, 2.26 mmol) as a pale-yellow liquid.
The title compound (500 mg) was prepared from 4-bromo-3,5-difluoroaniline (300 mg, 1.74 mmol) and 3-(ethylthio)phenylboronic acid (340 mg, 1.86 mmol) as a yellow liquid.
The title compound (300 mg) was prepared from 4-bromoaniline (270 mg, 1.56 mmol) and 3-ethylphenylboronic acid (300 mg, 2.04 mmol) as a colourless liquid.
The title compound (250 mg) was prepared from 4-bromo-2,3,5,6-tetrafluoroaniline (300 mg, 1.23 mmol) and 3-butoxyphenylboronic acid (310 mg, 1.59 mmol) as a yellow liquid.
The title compound (170 mg) was prepared from 4-bromo-2-fluoroaniline (240 mg, 1.26 mmol) and 3-butoxyphenylboronic acid (310 mg, 1.59 mmol) as a yellow liquid.
The title compound (230 mg) was prepared from 4-bromo-2-fluoroaniline (1 g, 5.26 mmol) and 3-isopropoxyphenylboronic acid (1.8 g, 6.84 mmol) as a yellow liquid.
The title compound (98 mg) was prepared from 4-bromoaniline (1 g, 5.81 mmol) and 3-isopropoxyphenylboronic acid (1.96 g, 7.55 mmol) as a yellow liquid.
The title compound (84 mg) was prepared from 4-bromoaniline (200 mg, 1.16 mmol) and 3-butoxyphenylboronic acid (293 mg, 1.5 mmol) as a yellow liquid.
The title compound (241 mg) was prepared from 4-bromo-3-fluoroaniline (200 mg, 1.05 mmol) and 3-butoxyphenylboronic acid (265 mg, 1.4 mmol) as a colourless liquid.
The title compound (172 mg) was prepared from 4-bromo-3,5-difluoroaniline (200 mg, 1 mmol) and 3-butoxyphenyl boronic acid (242 mg, 1.2 mmol) as a colourless liquid.
The title compound (500 mg) was prepared as a colourless viscous liquid by using the procedure followed for intermediate 64 from propyl magnesium bromide generated from propyl bromide (16.2 ml, 176.96 mmol) and magnesium (4.3 g, 176.96 mmol) in ether (50 ml), 5-bromoisatin (5 g, 22.12 mmol), THF (20 ml), 2M borane-dimethyl sulphide in THF (10 ml, 19.65 mmol), bis(pinacolato)diboron (600 mg, 2.3 mmol), potassium acetate (600 mg, 2.3 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]dichloro palladium(II).CH2Cl2 (44 mg, 0.03 mmol) and dioxane (10 ml).
The title compound (240 mg) was prepared from 4-bromo-2,6-difluoroaniline (400 mg, 1.55 mmol) and intermediate 85 (500 mg, 2.11 mmol) as a colourless gummy liquid.
The title compound (60 mg) was prepared from 4-bromo-2-chloro-6-fluoroaniline (300 mg, 1.1 mmol) and intermediate 64 (316 mg, 1.42 mmol) as a brown viscous liquid.
The title compound (134 mg) was prepared from 4-bromo-2-fluoroaniline (150 mg, 0.79 mmol) and 2-chloro-5-methoxylphenylboronic acid (191 mg, 1.02 mmol) as a colourless liquid.
The title compound (154 mg) was prepared from 4-bromoaniline (150 mg, 0.87 mmol) and 2-chloro-5-methoxyphenyl boronic acid (211 mg, 1.13 mmol) as a colourless viscous liquid.
General Procedure for Amide Formation:
Procedure-1
A solution of an anhydride (1.3 eq.) and an amine (1 eq) were dissolved in dioxane and refluxed overnight. Dioxane was evaporated and the resultant residue dissolved in AcOEt and extracted into aq. 2N NaHCO3 solution. The aqueous layer was acidified with aq. 2N HCl to obtain a solid, which was filtered and dried to give the desired amide.
Examples 1-11, 14, 16-17 and 20 were synthesised using general procedure-1.
Procedure-2
An amine (1 eq) was dissolved in toluene and an anhydride (1 eq) was added in portions and the mixture heated to 60° C. for 4 h. Solidified product was filtered and washed with aq. 2N HCl and dried under vacuum to obtain the desired product.
Examples 12 and 13 were synthesised using general procedure-2.
Procedure-3
An amine (1 eq) was dissolved in acetic acid and an anhydride (2 eq) was added and the mixture stirred at RT overnight. The solid that separated out was filtered and washed with petroleum ether and dried under vacuum to obtain the desired product.
Examples 15, 18, 19, 21-35, 38-71 and 73-100 were prepared using general procedure-3.
The title compound (52 mg) was obtained from intermediate 1 (150 mg, 0.64 mmol) and phthalic anhydride (189 mg) as a white solid. M.P.: 168-173° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.05 (s, 1H), 10.19 (s, 1H), 7.83 (d, J 7.4, 1H), 7.69-7.63 (m, 1H), 7.62-7.52 (m, 4H), 7.39 (t, J 7.9, 1H), 7.34-7.27 (m, 2H), 6.98 (d, J 6.8, 1H), 3.83 (s, 3H). MS (m/z): 381.55 ([M−H]−).
The title compound (64 mg) was obtained from intermediate 1 (150 mg, 0.64 mmol) and 2-sulphobenzoic acid cyclic anhydride (235 mg) as a brown solid. M.P.: 97-102° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 11.48 (s, 1H), 7.92 (d, J 7.1, 1H), 7.80 (d, J 7, 1H), 7.57-7.49 (m, 4H), 7.38 (t, J 7.8, 1H), 7.34-7.28 (m, 2H), 6.97 (d, J 8.1, 1H), 3.83 (s, 3H). MS (m/z): 417.6 ([M−H]−).
The title compound (48 mg) was obtained from intermediate 3 (100 mg, 0.5 mmol) and phthalic anhydride (147 mg, 1 mmol) as a white solid. M.P.: 194-199° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.17 (bs, 1H), 10.64 (s, 1H), 8.88 (d, J 2, 1H), 8.22 (dd, J 2.2, 8.7, 1H), 7.97 (d, J 8.7, 1H), 7.90 (d, J 7, 1H), 7.70-7.66 (m, 1H), 7.63-7.56 (m, 4H), 7.38 (t, J 8, 1H), 6.98-6.95 (m, 1H), 3.82 (s, 3H). MS (m/z): 347.30 ([M−H]−).
The title compound (26 mg) was obtained from intermediate 4 (100 mg, 0.5 mmol) and phthalic anhydride (128 mg, 1 mmol) as a white solid. M.P.: 151-157° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.06 (s, 1H), 10.22 (s, 1H), 7.95 (t, J 8.5, 1H), 7.89 (d, J 7.7, 1H), 7.68-7.52 (m, 5H), 7.35 (t, J 7.9, 1H), 7.25 (d, J 8.2, 1H), 7.21 (s, 1H), 6.92 (d, J 8.4, 1H), 4.10 (q, J 7, 2H), 1.34 (t, J 7, 3H). MS (m/z): 379.24 ([M]−).
The title compound (15 mg) was obtained from intermediate 5 (100 mg, 0.43 mmol) and pthalic anhydride (118 mg, 0.86 mmol) as a white solid. M.P.: 136-141° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.19 (s, 1H), 7.83 (d, J 7.4, 1H), 7.69-7.64 (m, 1H), 7.62-7.51 (m, 4H), 7.37 (t, J 7.9, 1H), 7.32-7.25 (m, 2H), 6.96 (dd, J 1.7, 8.3, 1H), 4.11 (q, J 7, 2H), 1.34 (t, J 7, 3H). MS (m/z): 395.81 ([M−H]−).
The title compound (270 mg) was obtained from intermediate 1 (150 mg, 0.64 mmol) and 2,3-pyrazinedicarboxylic anhydride (190 mg, 1.26 mmol) as a white solid. M.P.: 180.1-183.4° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.82 (bs, 1H), 10.7 (s, 1H), 8.92 (d, J 5.7, 2H), 7.59 (d, J 9.1, 2H), 7.42-7.38 (m, 1H), 7.36-7.28 (m, 2H), 7.00 (d, J 8, 1H), 3.83 (s, 3H). MS (m/z): 384.05 ([M−H]−).
The title compound (84 mg) was obtained from intermediate 5 (100 mg, 0.4 mmol) and 2,3-pyrazinedicarboxylic anhydride (120 mg, 0.8 mmol) as a white solid. M.P.: 133.4-137.3° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.76 (s, 1H), 10.68 (s, 1H), 8.92 (dd, J 2.3, 7.7, 2H), 7.58 (d, J 9.2, 2H), 7.41-7.36 (m, 1H), 7.34-7.26 (m, 2H), 6.98 (d, J 8, 1H), 4.11 (q, J 6.9, 2H), 1.35 (t, J 6.9, 3H). MS (m/z): 398.19 ([M−H]−).
The title compound (110 mg) was obtained from intermediate 6 (140 mg, 0.59 mmol) and phthalic anhydride (170 mg, 1.17 mmol) as a white solid. M.P.: 143.5-145.1° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.06 (s, 1H), 10.2 (s, 1H), 7.85 (d, J 7.6, 1H), 7.67 (t, J 7.3, 1H), 7.63-7.56 (m, 3H), 7.52-7.43 (m, 3H), 7.28 (d, J 8.4, 2H). MS (m/z): 386.15 ([M−H]−).
The title compound (42 mg) was obtained from intermediate 9 (65 mg, 0.21 mmol) and 2,3-Pyrazinedicarboxylic anhydride (62 mg, 0.42 mmol) as a white solid. M.P.: 142-144.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.77 (bs, 1H), 10.69 (s, 1H), 8.92 (dd, J 2.4, 7.7, 2H), 7.60 (d, J 9.2, 2H), 7.48 (d, J 7.3, 2H), 7.44-7.32 (m, 6H), 7.07 (d, J 6.5, 1H), 5.20 (s, 2H). MS (m/z): 460.28 ([M−H]−).
The title compound (85 mg) was obtained from intermediate 7 (100 mg, 0.48 mmol) and phthalic anhydride (144 mg, 0.86 mmol) as a white solid. M.P.: 161.2-165.7° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.19 (s, 1H), 7.83 (d, J 7.6, 1H), 7.76 (d, J 7.4, 2H), 7.65 (d, J 8.2, 1H), 7.62-7.48 (m, 6H), 7.43 (d, J 7.2, 1H). MS (m/z): 352.06 ([M−H]−).
The title compound (30 mg) was obtained from intermediate 10 (100 mg, 0.38 mmol) and 2,3-pyrazinedicarboxylic anhydride (112 mg, 0.75 mmol) as a white solid. M.P.: 148-153° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.74 (bs, 1H), 10.72 (s, 1H), 8.93 (dd, J 2.3, 6.4, 2H), 7.76 (s, 1H), 7.70 (d, J 10.7, 1H), 7.42-7.36 (m, 1H), 7.34-7.26 (m, 2H), 6.98 (dd, J 1.8, 8.1, 1H), 4.12 (q, J 6.9, 2H), 1.34 (t, J 6.9, 3H). MS (m/z): 414.01 ([M−H]−).
The title compound (15 mg) was obtained from intermediate 8 (100 mg, 0.34 mmol) and phthalic anhydride (50 mg, 0.34 mmol) as a white solid. M.P.: 148.2-151.4° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.07 (s, 1H), 10.24 (s, 1H), 7.83 (t, J 6.2, 2H), 7.79 (s, 1H), 7.54-7.50 (m, 6H), 7.42 (d, J 8.8, 1H). MS (m/z): 436.13 ([M−H]−).
The title compound (12 mg) was obtained from intermediate 9 (100 mg, 0.32 mmol) and phthalic anhydride (47 mg, 0.32 mmol) as a white solid. M.P.: 140.3-143.4° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.19 (s, 1H), 7.83 (d, J 7.6, 1H), 7.68-7.64 (m, 1H), 7.62-7.53 (m, 4H), 7.48 (d, J 7.2, 2H), 7.42-7.37 (m, 4H), 7.36-7.32 (m, 2H), 7.05 (dd, J 2, 7.7, 1H), 5.20 (s, 2H). MS (m/z): 457.83 ([M−H]−).
The title compound (42 mg) was obtained from intermediate 10 (100 mg, 0.38 mmol) and phthalic anhydride (160 mg, 0.75 mmol) as an off-white solid. M.P.: 260-265° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 15.33 (s, 1H), 8.16 (s, 1H), 7.96 (s, 1H), 7.71 (s, 1H), 7.64 (d, J 11, 1H), 7.36 (t, J 7.8, 1H), 7.27-7.22 (m, 2H), 6.95 (dd, J 1.8, 8.1, 1H), 4.12 (d, J 7, 2H), 1.34 (t, J 7, 3H). MS (m/z): 481.02 ([M−H]−).
The title compound (86 mg) was obtained from intermediate 10 (145 mg, 0.55 mmol) and phthalic anhydride (160 mg, 1.1 mmol) as a white solid. M.P.: 135-140° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.23 (s, 1H), 7.82 (d, J 7.6, 1H), 7.72 (s, 1H), 7.70-7.58 (m, 4H), 7.38 (t, J 7.8, 1H), 7.33-7.26 (m, 2H), 6.97 (dd, J 2, 8.1, 1H), 4.12 (q, J 6.9, 2H), 1.35 (t, J 6.9, 3H). MS (m/z): 412.05 ([M−H]−).
The title compound (95 mg) was obtained from intermediate 1 (100 mg, 0.42 mmol) and 5,6-dichloroisobenzofuran-1,3-dione (182 mg, 0.84 mmol) as a white solid. M.P.: 158.6-162.2° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.58 (s, 1H), 10.40 (s, 1H), 7.57 (d, J 9.1, 2H), 8.03 (s, 1H), 7.83 (s, 1H), 7.39 (t, J 7.9, 1H), 7.34-7.28 (m, 2H), 6.98 (dd, J 1.9, 6.9, 1H), 3.83 (s, 3H). MS (m/z): 451.24 ([M−H]−).
The title compound (87 mg) was obtained from intermediate 5 (100 mg, 0.42 mmol) and 5,6-dichloroisobenzofuran-1,3-dione (173 mg, 0.8 mmol) as a white solid. M.P.: 190.1-192.4° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.58 (s, 1H), 10.39 (s, 1H), 8.03 (s, 1H), 7.82 (s, 1H), 7.56 (d, J 9.2, 2H), 7.37 (t, J 7.9, 1H), 7.33-7.26 (m, 2H), 6.97 (dd, J 1.8, 8.1, 1H), 4.11 (q, J 7, 2H), 1.34 (t, J 7, 3H). MS (m/z): 464.92 ([M−H]−).
The title compound (20 mg) was obtained from intermediate 11 (150 mg, 0.56 mmol) and phthalic anhydride (165 mg, 1.12 mmol) as a white solid. M.P.: 163-168° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.0 (s, 1H), 10.37 (s, 1H), 7.86 (s, 2H), 7.80 (d, J 7.5, 1H), 7.74-7.65 (m, 2H), 7.60 (t, J 7.5, 1H), 7.40 (t, J 7.9, 1H), 7.34-7.27 (m, 2H), 6.99 (d, J 7.5, 1H), 3.84 (s, 3H). MS (m/z): 415.15 ([M−H]−).
The title compound (38 mg) was obtained from intermediate 12 (100 mg, 0.36 mmol) and phthalic anhydride (105 mg, 0.72 mmol) as a white solid. M.P.: 144-148° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.24 (s, 1H), 7.82 (d, J 7.5, 1H), 7.73 (s, 1H), 7.70-7.57 (m, 4H), 7.38 (t, J 7.7, 1H), 7.32-7.26 (m, 2H), 6.98 (d, J 8.1, 1H), 4.02 (t, J 6.5, 2H), 1.77-1.72 (m, 2H), 0.99 (t, J 7.4, 3H). MS (m/z): 426.05 ([M−H]−).
The title compound (15 mg) was obtained from intermediate 13 (100 mg, 0.34 mmol) and phthalic anhydride (100 mg, 0.68 mmol) as a white solid. M.P.: 163-167° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.05 (s, 1H), 10.3 (s, 1H), 7.83 (d, J 7.6, 1H), 7.70-7.58 (m, 5H), 7.57-7.46 (m, 2H), 7.40-7.31 (m, 2H). MS (m/z): 386.15 ([M−H]−).
The title compound (30 mg) was obtained from intermediate 14 (150 mg, 0.53 mmol) and phthalic anhydride (157 mg, 1.06 mmol) as a white solid. M.P.: 135.9-138.2° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.0 (s, 1H), 10.37 (s, 1H), 7.86 (s, 2H), 7.80 (d, J 7.3, 1H), 7.74-7.64 (m, 2H), 7.62-7.55 (m, 1H), 7.38 (t, J 7.9, 1H), 7.32-7.26 (m, 2H), 7.00-6.96 (m, 1H), 4.12 (q, J 7, 2H), 1.34 (t, J 7, 3H). MS (m/z): 429.30 ([M−H]−).
The title compound (124 mg) was obtained from intermediate 15 (100 mg, 0.44 mmol) and phthalic anhydride (130 mg, 0.9 mmol) as a white solid. M.P.: 128.3-132.1° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.07 (s, 1H), 10.28 (s, 1H), 7.99 (t, J 8.3, 1H), 7.88 (d, J 7.4, 1H), 7.77 (d, J 8, 1H), 7.67 (s, 1H), 7.69-7.52 (m, 6H), 7.37 (d, J 8.2, 1H). MS (m/z): 417.86 ([M−H]−).
The title compound (82 mg) was obtained from intermediate 16 (100 mg, 0.36 mmol) and phthalic anhydride (109 mg, 0.72 mmol) as a white solid. M.P.: 142.2-148.1° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.08 (s, 1H), 10.36 (s, 1H), 8.03 (d, J 8.2, 1H), 7.90 (d, J 7.8, 1H), 7.68 (t, J 7.5, 1H), 7.65-7.54 (m, 4H), 7.49-7.41 (m, 2H), 7.38-7.30 (m, 2H). MS (m/z): 418.00 ([M−H]−).
The title compound (78 mg) was obtained from intermediate 17 (100 mg, 0.39 mmol) and phthalic anhydride (115 mg, 0.78 mmol) as a white solid. M.P.: 185.4-193.6° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.42 (s, 1H), 7.81 (d, J 7.4, 1H), 7.74 (s, 2H), 7.72-7.58 (m, 4H), 7.53-7.47 (m, 1H), 7.40-7.31 (m, 2H). MS (m/z): 402.11 ([M−H]−).
The title compound (64 mg) was obtained from intermediate 18 (100 mg, 0.38 mmol) and phthalic anhydride (112 mg, 0.76 mmol) as a white solid. M.P.: 122-125° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.05 (s, 1H), 10.20 (s, 1H), 7.83 (d, J 7.5, 1H), 7.66 (t, J 7.3, 1H), 7.60-7.52 (m, 4H), 7.37 (t, J 7.8, 1H), 7.30-7.24 (m, 2H), 6.96 (dd, J 2.1, 8.2, 1H), 4.76 (septet, J 6.0, 1H), 1.28 (d, J 6, 6H). MS (m/z): 410.02 ([M−H]−).
The title compound (68 mg) was obtained from intermediate 19 (100 mg, 0.38 mmol) and phthalic anhydride (112 mg, 0.76 mmol) as a white solid. M.P.: 128.6-132.1° C. 1H-NMR (8 ppm, DMSO-d6, 400 MHz): 13.05 (s, 1H), 10.19 (s, 1H), 7.83 (d, J 7.7, 1H), 7.66 (t, J 7.4, 1H), 7.62-7.52 (m, 4H), 7.37 (t, J 7.8, 1H), 7.32-7.26 (m, 2H), 6.97 (dd, J 2, 8.1, 1H), 4.01 (t, J 6.5, 2H), 1.77-1.67 (m, 2H), 0.99 (t, J 7.4, 3H). MS (m/z): 410.16 ([M−H]−).
The title compound (110 mg) was obtained from intermediate 20 (100 mg, 0.39 mmol) and 5,6-dichloroisobenzofuran-1,3-dione (169 mg, 0.78 mmol) as a white solid. M.P.: 202.3-206.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.60 (s, 1H), 10.50 (s, 1H), 8.04 (s, 1H), 7.82 (s, 1H), 7.63-7.58 (m, 1H), 7.52-7.43 (m, 5H). MS (m/z): 471.69 ([M−H]−).
The title compound (51 mg) was obtained from intermediate 5 (100 mg, 0.4 mmol) and 4,7-dichloroisobenzofuran-1,3-dione (173 mg, 0.8 mmol) as a white solid. M.P.: 126.9-131.4° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.96 (s, 1H), 10.56 (s, 1H), 7.68 (s, 2H), 7.55 (d, J 9.1, 2H), 7.38 (t, J 7.9, 1H), 7.32-7.26 (m, 2H), 6.97 (dd, J 1.7, 12.2, 1H), 4.12 (q, J 7, 2H), 1.34 (t, J 7, 3H).
The title compound (39 mg) was obtained from intermediate 21 (90 mg, 0.31 mmol) and phthalic anhydride (90 mg, 0.6 mmol) as a white solid. M.P.: 128-130° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.23 (s, 1H), 7.82 (d, J 7.9, 1H), 7.73 (s, 1H), 7.60-7.57 (m, 4H), 7.37 (t, J 7.9, 1H), 7.32-7.25 (m, 2H), 6.99-6.96 (m, 1H), 4.06 (t, J 6.4, 2H), 1.73-1.68 (m, 2H), 1.45 (h, J 7.5, 2H), 0.94 (t, J 7.4, 3H). MS (m/z): 440.19 ([M−H]−).
The title compound (150 mg) was obtained from intermediate 6 (100 mg, 4.18 mmol) and 5,6-dichloroisobenzofuran-1,3-dione (181 mg, 8.4 mmol) as a white solid. M.P.: 202-205.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.61 (s, 1H), 10.45 (s, 1H), 8.04 (s, 1H), 7.83 (s, 1H), 7.62-7.58 (m, 1H), 7.52-7.49 (m, 1H), 7.48-7.43 (m, 2H), 7.30 (d, J 8.5, 2H). MS (m/z): 455.94 ([M−H]−).
The title compound (150 mg) was obtained from intermediate 22 (100 mg, 4.18 mmol) and 5,6-dichloroisobenzofuran-1,3-dione (181 mg, 8.4 mmol) as a white solid. M.P.: 202-205.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.23 (s, 1H), 7.82 (d, J 7.4, 1H), 7.73 (s, 1H), 7.70-7.57 (m, 4H), 7.38 (t, J 7.8, 1H), 7.32-7.26 (m, 2H), 6.99 (dd, J 1.7, 8.2, 1H), 3.83 (d, J 6.5, 2H), 2.10-1.99 (m, 1H), 1.00 (d, J 6.7, 6H). MS (m/z): 439.84 ([M−H]−).
The title compound (72 mg) was obtained from intermediate 23 (100 mg, 0.44 mmol) and phthalic anhydride (130 mg, 0.88 mmol) as a white solid. M.P.: 152.2-156.3° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.06 (s, 1H), 10.25 (s, 1H), 7.84 (d, J 7.6, 1H), 7.70-7.56 (m, 4H), 7.52-7.45 (m, 1H), 7.43-7.38 (m, 2H), 7.36-7.31 (m, 2H). MS (m/z): 369.91 ([M−H]−).
The title compound (20 mg) was obtained from intermediate 24 (100 mg, 0.37 mmol) and phthalic anhydride (130 mg, 0.88 mmol) as a white solid. M.P.: 182-185° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.01 (s, 1H), 10.40 (s, 1H), 7.82 (d, J 7.2, 1H), 7.72-7.67 (m, 2H), 7.64-7.59 (m, 4H), 7.53-7.50 (m, 1H), 7.49-7.45 (m, 2H). MS (m/z): 419.61 ([M−H]−).
The title compound (34 mg) was obtained from intermediate 25 (100 mg, 0.36 mmol) and phthalic anhydride (106 mg, 0.72 mmol) as a white solid. M.P.: 112.6-116.4° C. 1H-NMR (8 ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.18 (s, 1H), 7.83 (d, J 7.5, 1H), 7.68-7.64 (m, 1H), 7.62-7.52 (m, 4H), 7.37 (t, J 7.8, 1H), 7.33-7.25 (m, 2H), 6.98 (d, J 8.2, 1H), 3.84 (d, J 6.5, 2H), 2.10-1.99 (m, 1H), 1.00 (d, J 6.7, 6H). MS (m/z): 423.95 ([M−H]−).
The title compound (32 mg) was obtained from intermediate 26 (100 mg, 0.36 mmol) and phthalic anhydride (106 mg, 0.72 mmol) as a white solid. M.P.: 119.3-123.3° C. 1H-NMR (8 ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.19 (s, 1H), 7.83 (d, J 7.4, 1H), 7.69-7.63 (m, 1H), 7.62-7.52 (m, 4H), 7.37 (t, J 7.9, 1H), 7.32-7.26 (m, 2H), 6.97 (dd, J 1.6, 8, 1H), 4.05 (t, J 6.4, 2H), 1.72 (q, J 6.4, 2H), 1.47 (h, J 7.5, 2H), 0.94 (t, J 7.3, 3H). MS (m/z): 423.88 ([M−H]−).
Oxalyl chloride (0.86 ml, 9.8 mml) and two drops of DMF were added to a solution of 2-(methoxycarbonyl)benzoic acid (590 mg, 3.27 mmol) in dichloromethane (10 ml) and cooled to 0° C. and stirred at RT for 30 min. After 30 min., the solvent was removed to obtain methyl 2-(chloroformyl)benzoate (quantitative). A solution of this intermediate (401 mg, 2.03 mmol) in dichloromethane was added to a solution of intermediate 10 (540 mg, 2.03 mmol) and pyridine (0.19 ml, 2.43 mmol) in dichloromethane (5 ml) at 0° C. and stirred at RT for 30 min. Work-up (CH2Cl2/H2O) and purification gave methyl 2-(3-chloro-3′-ethoxy-5-fluorobiphenyl-4-ylcarbamoyl)benzoate (250 mg) as an off-white solid. Lithium borohydride (20 mg, 0.94 mmol) was added to a solution of this product (200 mg, 0.47 mmol) in THF (5 ml) at 0° C. and the mixture stirred at RT for 2 hrs. Work-up (EtOAc/aq. 10% NH4Cl then H2O) and purification afforded the title compound (30 mg) as a white solid. M.P.: 139.2-141.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 10.22 (s, 1H), 7.76 (s, 1H), 7.73-7.60 (m, 3H), 7.54 (t, J 6.7, 1H), 7.43-7.36 (m, 2H), 7.33-7.26 (m, 2H), 6.97 (dd, J 1.8, 8.1, 1H), 5.32 (t, J 5.6, 1H), 4.72 (d, J 5.6, 2H), 4.12 (q, J 7, 2H), 1.35 (t, J 7, 3H).
A solution of methyl 2-(chloroformyl)benzoate (prepared as described under example 37, 361 mg, 2.0 mmol) in dichloromethane was added slowly to a solution of intermediate 5 (500 mg, 2.0 mmol) and pyridine (0.19 ml, 2.43 mmol) in dichloromethane (5 ml) and stirred at 0° C. and stirred at RT for 30 min. Work-up (CH2Cl2/H2O) and purification gave methyl 2-(3′-ethoxy-3,5-difluorobiphenyl-4-ylcarbamoyl)benzoate (212 mg) as a pale-yellow solid. Lithium borohydride (21 mg, 0.96 mmol) was added to a solution of this intermediate (200 mg, 0.48 mmol) in THF (3 ml) at 0° C. and the mixture stirred at RT for 2 hrs. work-up (EtOAc/aq. 10% NH4Cl then H2O) and purification gave the title compound (21 mg) as a white solid. M.P.: 94.8-99.1° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 10.16 (s, 1H), 7.64 (d, J 7.8, 1H), 7.61-7.51 (m, 4H), 7.42-7.35 (m, 2H), 7.32-7.26 (m, 2H), 6.97 (dd, J 2, 7.6, 1H), 5.31 (t, J 5.6, 1H), 4.70 (d, J 5.6, 2H), 4.11 (q, J 7, 2H), 1.35 (t, J 7, 3H).
The title compound (20 mg) was obtained from intermediate 10 (100 mg, 0.38 mmol) and 4-fluoroisobenzofuran-1,3-dione (125 mg, 0.75 mmol) as a white solid. M.P.: 156.3-158.2° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.42 (s, 1H), 10.44 (s, 1H), 7.79-7.69 (m, 2H), 7.59-7.50 (m, 3H), 7.42-7.34 (m, 1H), 7.32-7.24 (m, 2H), 6.97 (d, J 7.6, 1H), 4.12 (t, J 7, 2H), 1.33 (q, J 7, 3H).
The title compound (38 mg) was obtained from intermediate 27 (150 mg, 0.6 mmol) and phthalic anhydride (176 mg, 1.2 mmol) as a white solid. M.P.: 153-156.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.28 (s, 1H), 7.87-7.73 (m, 5H), 7.69-7.55 (m, 4H), 7.43 (d, J 7.6, 1H). MS (m/z): 451.95 ([M−H]−).
The title compound (134 mg) was obtained from intermediate 29 (160 mg, 0.65 mmol) and phthalic anhydride (193 mg, 1.3 mmol) as a white solid. M.P.: 176.2-180.6° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 12.98 (bs, 1H), 9.88 (s, 1H), 7.79 (d, J 7.6, 1H), 7.67-7.61 (m, 1H), 7.60-7.52 (m, 2H), 7.45 (d, J 7, 2H), 7.40 (t, J 7, 2H), 7.37-7.32 (m, 1H), 6.90 (d, J 9.4, 2H), 5.14 (s, 2H).
The title compound (38 mg) was obtained from intermediate 32 (150 mg, 0.6 mmol) and phthalic anhydride (176 mg, 1.2 mmol) as a white solid. M.P.: 153-156.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.05 (s, 1H), 10.18 (s, 1H), 7.83 (d, J 7.6, 1H), 7.69-7.64 (m, 1H), 7.62-7.49 (m, 4H), 7.36 (t, J 8, 1H), 7.30-7.25 (m, 1H), 7.23 (m, 1H), 6.95 (dd, J 2, 8.1, 1H), 5.00-4.92 (m, 1H), 2.00-1.90 (m, 2H), 1.76-1.68 (m, 4H), 1.64-1.54 (m, 2H). MS (m/z): 436.13 ([M−H]−).
The title compound (38 mg) was obtained from intermediate 33 (100 mg, 0.33 mmol) and phthalic anhydride (96 mg, 0.65 mmol) as a white solid. M.P.: 122-126° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.23 (s, 1H), 7.82 (d, J 7.4, 1H), 7.71 (s, 1H), 7.68-7.58 (m, 4H), 7.37 (t, J 7.6, 1H), 7.30-7.21 (m, 2H), 6.95 (dd, J 2.2, 8.1, 1H), 5.00-4.93 (s, 1H), 2.00-1.88 (m, 2H), 1.80-1.68 (m, 4H), 1.63-1.54 (m, 2H). MS (m/z): 452.16 ([M−H]−).
The title compound (36 mg) was obtained from intermediate 34 (40 mg, 0.15 mmol) and phthalic anhydride (43 mg, 0.29 mmol) as a white solid. M.P.: 148.2-150.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.05 (s, 1H), 10.22 (s, 1H), 7.84 (d, J 7.7, 1H), 7.69-7.51 (m, 8H), 7.36 (t, J 74, 1H), 7.22 (dd, J 1.8, 9.7, 1H).
The title compound (41 mg) was obtained from intermediate 35 (40 mg, 0.14 mmol) and phthalic anhydride (41 mg, 0.28 mmol) as a white solid. M.P.: 156.5-159.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.26 (s, 1H), 7.83 (d, J 7.6, 1H), 7.79 (s, 1H), 7.75-7.70 (m, 1H), 7.69-7.58 (m, 5H), 7.54 (t, J 6.1, 1H), 7.37 (t, J 74, 1H), 7.22 (dd, J 1.8, 7.8, 1H).
The title compound (41 mg) was obtained from intermediate 36 (40 mg, 0.15 mmol) and phthalic anhydride (44 mg, 0.3 mmol) as a white solid. M.P.: 167-172° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.06 (s, 1H), 10.23 (s, 1H), 7.85 (d, J 7.6, 1H), 7.69-7.65 (m, 1H), 7.62-7.55 (m, 2H), 7.48 (d, J 8.6, 1H), 7.29 (d, J 8.3, 2H), 7.08-7.00 (m, 2H), 2.49 (s, 3H).
The title compound (37 mg) was obtained from intermediate 37 (40 mg, 0.15 mmol) and phthalic anhydride (46 mg, 0.31 mmol) as a white solid. M.P.: 166.2-167.8° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.22 (s, 1H), 7.84 (d, J 7.6, 1H), 7.70-7.52 (m, 5H), 7.23 (d, J 9.8, 1H), 7.18 (s, 1H), 6.88 (d, J 10.8, 1H), 3.85 (s, 3H).
The title compound (37 mg) was obtained from intermediate 38 (40 mg, 0.15 mmol) and phthalic anhydride (46 mg, 0.31 mmol) as a white solid. M.P.: 166.2-167.8° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.14 (s, 1H), 7.83 (d, J 5.9, 1H), 7.67-7.64 (m, 1H), 7.60-7.56 (m, 2H), 7.47 (d, J 9.2, 2H), 7.38 (s, 1H), 7.26 (dd, J 1.5, 8.2, 1H), 7.01 (d, J 8.1, 1H), 6.07 (s, 2H).
The title compound (23 mg) was obtained from intermediate 39 (50 mg, 0.19 mmol) and phthalic anhydride (55 mg, 0.38 mmol) as a white solid. M.P.: 182-184.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.00 (s, 1H), 10.21 (s, 1H), 7.81 (d, J 7.5, 1H), 7.68-7.56 (m, 5H), 7.39 (s, 1H), 7.26 (dd, J 1.4, 9.4, 1H), 7.01 (d, J 8.1, 1H), 6.08 (s, 2H).
The title compound (23 mg) was obtained from intermediate 40 (50 mg, 0.19 mmol) and phthalic anhydride (55 mg, 0.38 mmol) as a white solid. M.P.: 182-184.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.14 (s, 1H), 7.83 (d, J 7.6, 1H), 7.67-7.64 (m, 1H), 7.58 (t, J 7.1, 2H), 7.52 (d, J 9.3, 2H), 7.33-7.28 (m, 2H), 7.04 (d, J 9, 1H), 3.86 (s, 3H), 3.79 (s, 3H).
The title compound (35 mg) was obtained from intermediate 41 (40 mg, 0.14 mmol) and phthalic anhydride (44 mg, 0.28 mmol) as a white solid. M.P.: 164-166° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.25 (s, 1H), 7.85-7.78 (m, 2H), 7.73 (d, J 11.2, 1H), 7.69-7.56 (m, 3H), 7.24 (d, J 9.9, 1H), 7.18 (s, 1H), 6.88 (d, J 10.8, 1H), 3.85 (s, 3H).
The title compound (41 mg) was obtained from intermediate 42 (40 mg, 0.14 mmol) and phthalic anhydride (44 mg, 0.28 mmol) as a white solid. M.P.: 159.6-161.2° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.01 (s, 1H), 10.25 (s, 1H), 7.82 (d, J 7.9, 1H), 7.79 (s, 1H), 7.74 (d, J 10.7, 1H), 7.69-7.57 (m, 3H), 7.43 (s, 1H), 7.28 (s, 1H), 7.08 (s, 1H), 3.86 (s, 3H).
The title compound (46 mg) was obtained from intermediate 43 (50 mg, 0.2 mmol) and phthalic anhydride (59 mg, 0.40 mmol) as a white solid. M.P.: 176-177.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.12 (s, 1H), 7.83 (d, J 7.6, 1H), 7.67 (s, 2H), 7.61-7.53 (m, 2H), 7.49 (d, J 7.6, 1H), 7.43 (d, J 9.2, 2H), 6.84 (d, J 8.3, 1H), 4.57 (t, J 8.7, 2H), 3.23 (t, J 8.6, 2H).
The title compound (44 mg) was obtained from intermediate 44 (40 mg, 0.15 mmol) and phthalic anhydride (44 mg, 0.30 mmol) as a white solid. M.P.: 177-179° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.01 (s, 1H), 10.18 (s, 1H), 7.82 (d, J 7.6, 1H), 7.69-7.55 (m, 6H), 7.50 (d, J 8.2, 1H), 6.85 (d, J 8.4, 1H), 4.58 (t, J 8.8, 2H), 3.23 (t, J 8.8, 2H).
The title compound (16 mg) was obtained from intermediate 45 (25 mg, 0.09 mmol) and phthalic anhydride (27 mg, 0.18 mmol) as a white solid. M.P.: 276.4-277.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.14 (s, 1H), 8.14 (s, 1H), 7.83 (d, J 7.2, 1H), 7.79 (d, J 9, 1H), 7.68-7.57 (m, 6H), 3.98 (s, 1H), 2.53 (s, 3H).
The title compound (37 mg) was obtained from intermediate 46 (35 mg, 0.13 mmol) and phthalic anhydride (39 mg, 0.25 mmol) as a white solid. M.P.: 171-175° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.22 (s, 1H), 7.83 (d, J 7.7, 1H), 7.70-7.55 (m, 5H), 7.42 (s, 1H), 7.28 (s, 1H), 7.07 (s, 1H), 3.85 (s, 3H).
The title compound (24 mg) was obtained from intermediate 47 (30 mg, 0.1 mmol) and phthalic anhydride (31 mg, 0.2 mmol) as a white solid. M.P.: 179.6-182.1° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.0 (s, 1H), 10.18 (s, 1H), 7.82 (d, J 7.7, 1H), 7.72 (s, 1H), 7.68-7.57 (m, 4H), 7.33-7.28 (m, 2H), 7.04 (d, J 8.8, 1H), 3.86 (s, 3H), 3.79 (s, 3H).
The title compound (54 mg) was obtained from intermediate 48 (30 mg, 0.1 mmol) and phthalic anhydride (30 mg, 0.2 mmol) as a white solid. M.P.: 165-167° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.27 (s, 1H), 7.83 (d, J 7.4, 1H), 7.70-7.57 (m, 3H), 7.51-7.47 (m, 2H), 7.43 (d, J 10, 1H), 7.08-7.01 (m, 2H), 3.80 (s, 3H).
The title compound (23 mg) was obtained from intermediate 49 (30 mg, 0.1 mmol) and phthalic anhydride (38 mg, 0.22 mmol) as a white solid. M.P.: 173.4-176.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.24 (s, 1H), 7.84 (d, J 7.7, 1H), 7.67 (t, J 7.1, 1H), 7.60 (t, J 8, 2H), 7.41 (d, J 8.6, 2H), 7.27 (t, J 9.5, 1H), 7.15-7.12 (m, 1H), 7.03-6.96 (m, 1H), 3.80 (s, 3H).
The title compound (42 mg) was obtained from intermediate 50 (80 mg, 0.3 mmol) and phthalic anhydride (85 mg, 0.6 mmol) as a white solid. M.P.: 156.1-158.3° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.21 (s, 1H), 7.84 (d, J 12.6, 2H), 7.70-7.57 (m, 4H), 7.55-7.49 (m, 1H), 7.46 (s, 1H), 7.34 (d, J 8.1, 1H), 3.97 (s, 3H).
The title compound (63 mg) was obtained from intermediate 51 (80 mg, 0.27 mmol) and phthalic anhydride (83 mg, 0.55 mmol) as a white solid. M.P.: 147.6-150.4° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.24 (s, 1H), 7.85-7.79 (m, 2H), 7.73 (d, J 7.8, 1H), 7.70-7.58 (m, 3H), 7.51 (d, J 8.1, 1H), 7.46 (s, 1H), 7.34 (d, J 6.5, 1H), 3.97 (s, 3H).
The title compound (40 mg) was obtained from intermediate 52 (70 mg, 0.26 mmol) and phthalic anhydride (76 mg, 0.52 mmol) as an off-white solid. M.P.: 160.1-163.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.05 (s, 1H), 10.28 (s, 1H), 7.83 (d, J 7.3, 1H), 7.70-7.51 (m, 5H), 7.28 (d, J 9.5, 1H), 7.17-7.11 (m, 1H), 7.05-6.97 (m, 1H), 3.81 (s, 3H).
The title compound (60 mg) was obtained from intermediate 53 (200 mg, 0.79 mmol) and phthalic anhydride (230 mg, 1.58 mmol) as an off-white solid. M.P.: 125.6-128.8° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.18 (s, 1H), 7.83 (d, J 7.6, 1H), 7.68-7.64 (m, 1H), 7.63-7.54 (m, 4H), 7.50 (d, J 8, 1H), 7.35-7.28 (m, 2H), 3.94 (s, 3H).
The title compound (84 mg) was obtained from intermediate 54 (66 mg, 0.25 mmol) and phthalic anhydride (75 mg, 0.51 mmol) as a white solid. M.P.: 182-186.5° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.86 (s, 1H), 10.11 (s, 1H), 7.87 (s, 1H), 7.83 (d, J 7.3, 1H), 7.69-7.63 (m, 1H) 7.62-7.57 (m, 2H), 7.52 (d, J 9.3, 2H), 7.45 (d, J 8.5, 1H), 7.40 (d, J 8.4, 1H), 7.15 (s, 1H), 2.31 (s, 3H).
The title compound (38 mg) was obtained from intermediate 55 (50 mg, 0.19 mmol) and phthalic anhydride (57 mg, 0.39 mmol) as a white solid. M.P.: 179-184° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 12.79 (bs, 1H), 10.15 (s, 1H), 8.14 (s, 1H), 7.84 (d, J 7.3, 1H), 7.73 (d, J 7.3, 1H), 7.69-7.65 (m, 1H), 7.62-7.55 (m, 4H), 7.53 (d, J 8.7, 1H), 6.02 (bs, 1H), 2.54 (s, 3H).
The title compound (38 mg) was obtained from intermediate 56 (50 mg, 0.19 mmol) and phthalic anhydride (57 mg, 0.39 mmol) as a white solid. M.P.: 179-184° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (bs, 1H), 10.22 (s, 1H), 7.82 (d, J 7.7, 1H), 7.74-7.54 (m, 7H), 7.39 (t, J 7.6, 1H), 7.27 (d, J 7.6, 1H). 2.68 (q, J 7.6, 2H), 1.23 (t, J 7.6, 3H).
The title compound (54 mg) was obtained from intermediate 57 (60 mg, 0.21 mmol) and phthalic anhydride (63 mg, 0.42 mmol) as a white solid. M.P.: 160-163° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.29 (s, 1H), 7.83 (d, J 7.6, 1H), 7.70-7.55 (m, 4H), 7.51 (d, J 10, 1H), 7.26-7.21 (m, 2H), 7.16-7.10 (m, 1H), 4.14 (q, J 6.8, 2H), 1.37 (t, J 6.8, 3H).
The title compound (54 mg) was obtained from intermediate 58 (60 mg, 0.21 mmol) and phthalic anhydride (63 mg, 0.42 mmol) as a white solid. M.P.: 147-151° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.19 (s, 1H), 7.82 (d, J 7.5, 1H), 7.68-7.53 (m, 5H), 7.29 (d, J 2.1, 1H), 7.24 (dd, J 2.1, 8.4, 1H), 6.94 (d, J 8.4, 1H), 4.28 (s, 4H).
The title compound (21 mg) was obtained from intermediate 59 (80 mg, 0.25 mmol) and phthalic anhydride (74 mg, 0.5 mmol) as a white solid. M.P.: 133-137° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.25 (s, 1H), 7.86-7.79 (m, 2H), 7.72 (d, J 10.5, 1H), 7.70-7.55 (m, 3H), 7.48-7.42 (m, 3H), 7.15-7.06 (m, 1H), 4.88 (q, J 9.3, 2H).
The title compound (82 mg) was obtained from intermediate 60 (150 mg, 0.69 mmol) and phthalic anhydride (200 mg, 1.4 mmol) as a white solid. M.P.: 141-143° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.23 (s, 1H), 7.97-7.93 (m, 1H), 7.87 (d, J 7.5, 1H), 7.70-7.50 (m, 5H), 7.37 (t, J 7.9, 1H), 7.29-7.20 (m, 2H), 6.94 (d, J 6.1, 1H), 3.82 (s, 3H).
The title compound (82 mg) was obtained from intermediate 61 (150 mg, 0.69 mmol) and phthalic anhydride (200 mg, 1.4 mmol) as a white solid. M.P.: 141-143° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.41 (s, 1H), 7.87 (d, J 7.6, 1H), 7.76 (d, J 8.6, 2H), 7.69-7.61 (m, 3H), 7.60-7.52 (m, 2H), 7.33 (t, J 7.9, 1H), 7.20 (d, J 7.8, 1H), 7.14 (s, 1H), 6.87 (dd, J 2.1, 8.0, 1H), 4.07 (q, J 7, 2H), 1.34 (t, J 7, 2H).
The title compound (65 mg) was obtained from intermediate 62 (150 mg, 0.69 mmol) and phthalic anhydride (200 mg, 1.4 mmol) as a white solid. M.P.: 137.8-142.1° C. 1H-NMR (8 ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.2 (s, 1H), 7.84 (d, J 7.6, 1H), 7.70-7.52 (m, 7H), 7.42 (t, J 7.7, 1H), 7.34 (d, J 7.6, 1H), 3.08 (q, J 7.3, 2H), 1.26 (t, J 7.3, 3H). MS (m/z): 413.80 ([M+H]+).
Oxone (241 mg, 0.39 mmol) was added to a solution of example 71 (210 mg, 0.43 mmol) in water-acetone (1:1, 4.2 ml) and stirred at RT for 2 h. Solid that formed in reaction mixture was filtered and dried to obtain the title compound (95 mg) as a white solid. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.26 (s, 1H), 8.19 (s, 1H), 8.14 (d, J 7.8, 1H), 7.91 (d, J 7.8, 1H), 7.84 (d, J 7.3, 1H), 7.77 (t, J 7.8, 1H), 7.72-7.64 (m, 3H), 7.62-7.56 (m, 2H), 3.41 (q, J 7.3, 2H), 1.13 (t, J 7.3, 3H). MS (m/z): 430.07 ([M+H]+).
The title compound (96 mg) was obtained from intermediate 63 (160 mg, 0.61 mmol) and phthalic anhydride (181 mg, 1.2 mmol) as a white solid. M.P.: 125-127° C. MS (m/z): 410.2 ([M+H]+).
The title compound (75 mg) was obtained from intermediate 5 (200 mg, 0.8 mmol) and 4-methylisobenzofuran-1,3-dione (260 mg, 1.6 mmol) as a white solid. M.P.: 138-140° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.02 (s, 1H), 10.09 (s, 1H), 7.77 (d, J 7.8, 1H), 7.52 (d, J 9.3, 3H), 7.47-7.41 (m, 1H), 7.38 (t, J 7.9, 1H), 7.32-7.24 (m, 2H), 6.98-6.95 (m, 1H), 4.11 (q, J 6.9, 2H), 2.41 (s, 3H), 1.35 (t, J 6.9, 3H). MS (m/z): 410.07 ([M−H]−).
The title compound (75 mg) was obtained from intermediate 65 (250 mg, 0.992 mmol) and phthalic anhydride (271 mg, 1.84 mmol) as a white solid. M.P.: 157.9-160.9° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.03 (s, 1H), 10.88 (s, 1H), 10.11 (s, 1H), 7.89 (s, 1H), 7.83 (d, J 7.3, 1H), 7.69-7.63 (m, 1H), 7.62-7.56 (m, 2H), 7.51 (d, J 9.3, 2H), 7.47-7.39 (m, 2H), 7.16 (s, 1H), 2.75 (q, J 7.4, 2H), 1.28 (t, J 7.4, 3H). MS (m/z): 418.75 ([M−H]−).
The title compound (15 mg) was obtained from intermediate 5 (150 mg, 0.6 mmol) and furo[3,4-b]pyridine-5,7-dione (180 mg, 1.2 mmol) as a grey solid. M.P.: 207-210° C. MS (m/z): 399.08 ([M+H]+).
The title compound (10 mg) was obtained from intermediate 5 (100 mg, 0.4 mmol) and furo[3,4-c]pyridine-1,3-dione (119 mg, 0.8 mmol) as a white solid. M.P.: 219-221° C. MS (m/z): 399.01 ([M+H]+). The regiochemical assignment is based on earlier reports, e.g. Nailton et. al. Bioorganic & Medicinal Chemistry Letters 2010, 20(1), 74-77.
The title compound (82 mg) was obtained from intermediate 66 (280 mg, 0.93 mmol) and phthalic anhydride (206 mg, 1.4 mmol) as a white solid. M.P.: 158-160° C. MS (m/z): 448.29 ([M−H]−).
The title compound (50 mg) was obtained from intermediate 67 (210 mg, 0.83 mmol) and phthalic anhydride (185 mg, 1.2 mmol) as a grey solid. M.P.: 185-187° C. MS (m/z): 398.24 ([M−H]−).
The title compound (14 mg) was obtained from intermediate 68 (170 mg, 0.7 mmol) and phthalic anhydride (153 mg, 1.03 mmol) as a white solid. M.P.: 116-119° C. MS (m/z): 394.2 ([M+H]+).
The title compound (60 mg) was obtained from intermediate 69 (60 mg, 0.26 mmol) and phthalic anhydride (58 mg, 0.4 mmol) as a white solid. M.P.: 152-154° C. MS (m/z): 376.2 ([M+H]+).
The title compound (200 mg) was obtained from intermediate 70 (430 mg, 1.74 mmol) and phthalic anhydride (380 mg, 2.6 mmol) as a white solid. M.P.: 74-76° C. MS (m/z): 395.72 ([M+H]+).
The title compound (80 mg) was obtained from intermediate 71 (88 mg, 0.29 mmol) and phthalic anhydride (86 mg, 0.58 mmol) as a white solid. M.P.: 152-156° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.19 (s, 1H), 7.84 (d, J 7.2, 1H), 7.68-7.53 (m, 6H), 7.46-7.41 (m, 2H), 7.13-7.06 (m, 1H), 4.87 (q, J 9, 2H).
The title compound (15 mg) was obtained from intermediate 72 (170 mg, 0.73 mmol) and phthalic anhydride (210 mg, 1.45 mmol) as a white solid. M.P.: 132-136° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 13.04 (s, 1H), 10.18 (s, 1H), 7.83 (d, J 7.3, 1H), 7.69-7.48 (m, 7H), 7.39 (t, J 7.6, 1H), 7.26 (d, J 7.3, 1H), 2.67 (q, J 7.6, 2H), 1.23 (t, J 7.6, 3H).
The title compound (46 mg) was obtained from biphenyl-4-amine (65 mg, 0.38 mmol) and phthalic anhydride (85 mg, 0.58 mmol) as a white solid. M.P.: 276-278° C. MS (m/z): 318.1 ([M+H]+).
The title compound (223 mg) was obtained from intermediate 73 (170 mg, 0.83 mmol) and phthalic anhydride (185 mg, 1.25 mmol) as a white solid. M.P.: 243-247° C. MS (m/z): 352.1 ([M+H]+).
The title compound (223 mg) was obtained from intermediate 74 (170 mg, 0.83 mmol) and phthalic anhydride (185 mg, 1.25 mmol) as a white solid. M.P.: 243-247° C. MS (m/z): 348.2 ([M+H]+).
The title compound (110 mg) was obtained from intermediate 75 (280 mg, 1.1 mmol) and phthalic anhydride (245 mg, 1.66 mmol) as a white solid. M.P.: 154.4-158.5° C. MS (m/z): 402.2 ([M+H]+).
The title compound (50 mg) was obtained from intermediate 76 (240 mg, 0.9 mmol) and phthalic anhydride (200 mg, 1.35 mmol) as a white solid. M.P.: 162-167° C. MS (m/z): 414.2 ([M+H]+).
The title compound (96 mg) was obtained from intermediate 77 (100 mg, 0.5 mmol) and phthalic anhydride (110 mg, 0.76 mmol) as an off-white solid. M.P.: 178-182° C. MS (m/z): 346.2 ([M+H]+).
The title compound (130 mg) was obtained from intermediate 78 (180 mg, 0.57 mmol) and phthalic anhydride (127 mg, 0.86 mmol) as a white solid. M.P.: 215.5-218.5° C. MS (m/z): 460.18 ([M−H]−).
The title compound (130 mg) was obtained from intermediate 79 (170 mg, 0.65 mmol) and phthalic anhydride (145 mg, 0.98 mmol) as a white solid. M.P.: 102-105° C. MS (m/z): 406.17 ([M−H]−).
The title compound (50 mg) was obtained from intermediate 8 (200 mg, 0.69 mmol) and phthalic anhydride (150 mg, 1.03 mmol) as a white solid. M.P.: 158-160° C. MS (m/z): 435.93 ([M−H]−).
The title compound (120 mg) was obtained from intermediate 80 (100 mg, 0.41 mmol) and phthalic anhydride (91 mg, 0.62 mmol) as a white solid. M.P.: 134.5-137.5° C. MS (m/z): 390.27 ([M−H]−).
The title compound (90 mg) was obtained from intermediate 81 (98 mg, 0.43 mmol) and phthalic anhydride (96 mg, 0.65 mmol) as a white solid. M.P.: 150.2-154.3° C. MS (m/z): 372.14 ([M−H]−).
The title compound (37 mg) was obtained from intermediate 82 (80 mg, 0.33 mmol) and phthalic anhydride (73 mg, 0.5 mmol) as a white solid. M.P.: 155-157° C. MS (m/z): 388.38 ([M−H]−).
The title compound (171 mg) was obtained from intermediate 83 (240 mg, 0.92 mmol) and phthalic anhydride (205 mg, 1.3 mmol) as a white solid. M.P.: 164-167° C. MS (m/z): 406.31 ([M−H]−).
The title compound (91 mg) was obtained from intermediate 84 (170 mg, 0.6 mmol) and phthalic anhydride (136 mg, 0.9 mmol) as an off-white solid. M.P.: 181.1-184.2° C. MS (m/z): 423.95 ([M−H]−).
The title compound (150 mg) was obtained from intermediate 86 (240 mg, 0.84 mmol) and phthalic anhydride (186 mg, 1.25 mmol) as a white solid. M.P.: 76-80° C. 1H-NMR (δ ppm, DMSO-d6, 400 MHz): 10.88 (s, 1H), 7.88 (s, 1H), 7.81 (d, J 6.6, 1H), 7.75-7.71 (m, 1H), 7.53-7.47 (m, 5H), 7.44-7.38 (m, 2H), 7.14 (s, 1H), 2.72 (t, J 7.4, 2H), 1.69 (h, J 7.5, 2H), 0.96 (t, J 7.3, 3H). MS (m/z): 433.13 ([M−H]−).
The title compound (25 mg) was obtained from intermediate 87 (60 mg, 0.21 mmol) and phthalic anhydride (46 mg, 0.31 mmol) as a white solid. M.P.: 98-102° C. MS (m/z): 435.23 ([M−H]−).
Biological Assay
The properties of the compounds of this invention may be confirmed by a number of biological and pharmacological assays. The biological and pharmacological assay which can be been carried out with the compounds according to the invention and/or their pharmaceutically acceptable salts are exemplified below. Similarly the compounds of the present invention may also be tested using other assays such as cytokine (IL-17 and interferon gamma) estimation in human whole blood and PBMCs.
The compounds of the invention may also be tested in various animal models to establish the various therapeutic potential of the compounds of this invention.
1. In-Vitro DHODH Inhibition Assays
The properties of the compounds of this invention may be confirmed by a number of biological/pharmacological assays. The biological/pharmacological assay which can be been carried out with the compounds according to the invention and/or their pharmaceutically acceptable salts is exemplified below. Similarly the compounds of the present invention may also be tested using other assays such as cytokine (IL-17, interferon gamma etc.) estimation in human whole blood and PBMCs.
Dihydro-Orotate Dehydrogenase Inhibition Assay
Dihydro-orotate dehydrogenase (DHODH) catalyzes the reduction of dihydro-orotate to orotate during de novo biosynthesis of pyrimidines. Inhibition of DHODH activity in U937 membrane preparations was measured by the dihydro-orotate driven reduction of 2,6 dichloroindophenol (DCIP).
U937 cells were homogenized in 20 mM Tris/HCl (pH 7.2) containing 1 mM EDTA. Cell debris was removed by centrifugation at 2000×g for 10 min. Membrane fractions were pelleted by centrifuging the supernatant at 160000×g for 1 h at 4° C. and washed with buffer containing 125 mM sucrose and 150 mM NaCl. Following washes, the pellet was dissolved in 20 mM Tris/HCl containing 150 mM NaCl, 1 mM EDTA, and 1% octyl glucoside on ice for 1 h. Particulate matter was removed by centrifugation at 100000×g for 1 h at 4° C. Extracts (˜50 μg protein) were added to an assay mixture (200 μM CoQD, 500 μM dihydro-orotate, 75 μM DCIP in 100 mM HEPES pH 8.0, 150 mM NaCl, 10% glycerol, 0.05% Triton X-100) containing inhibitors at desired concentrations in a 96-well plate. The mixture was incubated at 37° C. for 4 h before measuring the change in absorbance on a plate reader (BMG Labtech., Germany) at 600 nm. Data were analyzed using GraphPad Prism. IC50 for each compound was determined based on the percent inhibition of dihydro-orotate reduction.
Results:
#compound tested at 10 uM;
2. Inhibition of IL-17 Release from Mouse Splenocytes:
Splenocytes isolated from Balb/c mice were re-suspended in RPMI medium at a concentration of 1×106 cells/ml and seeded in a 6 well plate. Cells were incubated with desired concentrations of the inhibitor for 15 min prior to induction with 10 ng/ml PMA+1 μM ionomycin. After a 3 h incubation, supernatant was collected and analyzed for IL-17 concentration using an ELISA kit. Data were analysed using GraphPad Prism. IC50 values for each compound were determined based on the percent inhibition due to the test compound compared to the control.
Results:
IC50
3. Determination of IL-17 Producing Cells by Flow Cytometry:
PBMC isolated from human blood were stimulated and treated with desired concentrations of the test compounds prior to stimulation with Cytostim (Milteny Biotech, Germany). After 4 h, IL-17 secreting cells were stained using the IL-17 secretion assay kit as per the manufacturer's instruction and normalized to total CD4+ cells within the PBMC population. Data were analyzed using Graph pad prism. For instance example 29 showed a 43.6% inhibition of IL17 when tested at at 1 uM. The results indicate the potential of the compounds of invention to inhibit IL17 release independent of DHODH inhibition.
4. In Vitro Inhibition of Proliferation and Cytokine Release in Peripheral Blood Mononuclear Cells (PBMC)
a. Inhibition of PHA Induced PBMC Proliferation:
PBMC from freshly collected HWB was isolated by density gradient using Histopaque and seeded in a 96-well plate. Wells were incubated with desired concentrations of the inhibitor for 15 min. Proliferation was induced by the addition of 2 μM Phytohemagglutinin at 37° C. in an atmosphere containing 95% CO2. Viability was determined after 48 h using an 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Data were analysed using GraphPad Prism. percent inhibition and/or GI50 values for each compound were determined based on the percent inhibition due to the test compound compared to the control. For instance example 29, 42 & 75 showed a greater than 70% inhibition when tested at 10 uM.
b. Inhibition of PHA Induced Cytokine (IL17) Release:
PBMC from freshly collected HWB were isolated by density gradient using Histopaque and seeded in a 96-well plate. Wells were incubated with desired concentrations of the inhibitor for 15 min. Proliferation was induced by the addition of 2 μM Phytohemagglutinin at 37° C. in an atmosphere containing 95% CO2. Supernatant was collected after 48 h for estimation of cytokines by ELISA. Data were analysed using GraphPad Prism. Percent inhibition and IC50 values for each compound were determined based on the percent inhibition due to the test compound compared to the control. For instance example 5 showed a greater than 50% inhibition when tested at 10 uM.
c. Inhibition of PHA Induced CD4+ Cell Proliferation in Human Whole Blood:
HWB or were treated with desired concentration of inhibitor and induced with 5 μM PHA. % CD4+ cell viability was determined after 48 h by flow cytometry. Data were analysed using GraphPad Prism. Percent inhibition and IC50 values for each compound were determined based on the percent inhibition due to the test compound compared to the control. For instance example 29 and 75 showed a greater than 65% inhibition when tested at 1 uM.
d. Inhibition of PHA Induced CD4+ Cell Proliferation in PBMC:
Isolated PBMC were treated with desired concentration of inhibitor and induced with 5 μM PHA. % CD4+ cell viability was determined after 48 h by flow cytometry. Data were analysed using GraphPad Prism. IC50 values for each compound were determined based on the percent inhibition due to the test compound compared to the control. For instance example 29 and 75 showed a greater than 90% inhibition when tested at 1 uM.
5. Single Dose Oral Hepatotoxicity Assay:
BALB/cJ (n=4 or 5/sex) mice aged 8 to 10 weeks, with weights ranging from 18 to 25 g were used. They were housed under conditions of controlled temperature and humidity and a 12-h light/dark cycle. They were given continuous access to bottled spring water and fed a standard chow at ad libitum. The mice were allowed to acclimate for 1 week before use. On the day of experiment mice were fasted overnight for 12 h and administered with test item formulation (100 mg/kg·b·wt/po) or vehicle (10 ml/kg·b·wt/po) by oral route and food was given 4 hr after test item administration. After 24 hr post administration blood samples were collected from orbital sinus of all the animals and serum was separated to assess the hepatotoxicity. Biochemical evaluation of liver function was determined by measuring serum enzyme activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) using commercially available kits from Sigma (St. Louis, Mo.).
Results:
The compounds of the invention were found to be Non-Hepatotoxic and the data is as disclosed herein below in Table-4.
6. Evaluation of Usefulness of DHODH Modulators in Various Anti-Inflammatory and Autoimmune Disorders Using In-Vivo Animal Models has been or can be Established Using the Methodology as Given Below.
i. Inhibition of Concanavalin Induced Lymphocyte Proliferation in Wistar Rats:
Con A is often used to prepare experimental animals with high levels of cytotoxic T-lymphocytes, because these cells are involved in the development of viral infections in humans. To evaluate the effect of an inhibitor on lymphocyte proliferation in rats, animals were treated with 10 mg/kg po of a compound of the present invention prior to intravenous administration of 5 mg/kg concanavalin A. Lymphocyte count was determined after 48 h on a Medonic blood analyzer. Data indicated a ˜75% reduction in peripheral blood lymphocytes upon treatment with the test compound implicating the therapeutic potential of the compound in immune-mediated disorders such as rheumatoid arthritis.
ii. Inhibition of Concanavalin Induced IL-17 Release in Balb/c Mice:
Balb/c mice were treated with 10 mg/kg po of the test compound prior to intravenous administration of 20 mg/kg concanavalin A. Plasma was obtained after 2 h and estimated for inhibition of IL-17 release by ELISA. The test compound reduced IL-17 secretion from Th17 cells in a dose-dependent manner.
iii. Inhibition of TNBS Induced Colitis in Balb/c Mice:
Female BALB/c Mice are to be fasted overnight and administered PBS, 50% Ethanol/PBS, or 50% Ethanol/20 mg/kg TNBS (40 μL enema), while under isoflourane anesthesia (study day 1). Animals are to be dosed p.o. with Vehicle, Dexamethasone (5 mg/kg), or test compounds at for example 25 mg/kg. The dosing would begin on study day 1 (5 hours after the TNBS enema). Mice are to be euthanized on day 7. Various parameters of colitis are to be measured, essentially according to a previously published study (see. Fitzpatrick et al., Inflammatory Bowel Diseases, 2010).
Other in-vivo models wherein the effect of DHODH modulators in various Anti-inflammatory and Autoimmune disorders can be tested include Collagen-induced arthritis in male DBA/a Ola HSD mice and Chronic Experimental Autoimmune Encephalomyelitis in C57/Bl6J mice: Collagen induced arthritis in rodent models have been widely used to illustrate and understand the development of the disease besides serving as a surrogate for validation of therapeutic targets for human rheumatoid arthritis. Mice are anesthetized with Isoflurane and given 150 μl of Bovine Type II collagen in Freund's complete adjuvant injections (day 0 and day 21). Treatment is initiated on study day 0 and continued once daily, every day (po, qd). Starting on day 18, clinical scores are given daily for each of the paws (right front, left front, right rear, left rear) and continued till the day of sacrifice (day 34).
Experimental Autoimmune Encephalomyelitis (EAE) is an inflammatory disease of the central nervous system and widely used as an animal model of Multiple Sclerosis. Animals are administered pertussis toxin intravenously and myelin oligodendrocyte glycoprotein (MOG) subcutaneously on day 0. Treatment is initiated at day 0 and continued till sacrifice. Development of EAE is observed between day 9 to day 42. At the end of the treatment period, animals are sacrificed for histopathological analysis as well as cytokine estimation in plasma.
Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and applications of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as described above and the appended claim.
All publications and patent and/or patent applications cited in this application are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated herein by reference.
| Number | Date | Country | Kind |
|---|---|---|---|
| 1265/CHE/2010 | May 2010 | IN | national |
This application is a continuation of U.S. patent application Ser. No. 13/101,921, filed May 5, 2011, which claims the benefit of Indian Provisional Patent Application No. 1265/CHE/2010 dated 6th May 2010, each of which is hereby incorporated by reference.
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| Number | Date | Country | |
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| Parent | 13101921 | May 2011 | US |
| Child | 14172707 | US |
