Claims
- 1. A method of labelling protein comprising the step of covalently bonding the protein to a microsphere composition comprising
- polymeric microspheres having a diameter below 100 microns containing at least 20% by weight of a tertiary amine substituted monomer having the amine ligand pendant from the polymer backbone selected from the group consisting of cross-linked polystyrene-divinylbenzene substituted with --CH.sub.2 NMe.sub.2, polyethylene imine, polyvinylbenzimidazole and polyvinylpyridine, said ligands being capable of ionic reaction or coordination with Group IV, VI, VII or VIII metal compounds selected from oxides, salts or acids to form complexes therewith and said microspheres containing; at least 0.1 weight percent of finely divided, free metal associated with the amine ligands formed by reduction of the compound in situ within the microsphere by reaction with a reducing agent selected from the group consisting of borohydrides and aluminum hydrides and at least 20% by weight of an acrylic monomer having a covalent bonding group selected from hyroxyl, carboxyl or amine.
- 2. A method according to claim 1 in which the metal is a heavy metal.
- 3. A method according to claim 2 further including the step of separating microsphere labelled protein from nonlabelled protein.
- 4. A method according to claim 1 in which the tertiary amine substituted monomer is vinyl pyridine.
- 5. A method according to claim 4 in which the microspheres contain from 5 to 50% by weight of metal.
- 6. A method according to claim 1 in which the metal is selected from iron, gold, platinum, palladium, cobalt, nickel, rhodium manganese, chromium, titantium, ruthenium, tantalum or iridium.
- 7. A method according to claim 6 in which the metal is a transition metal which forms a coordination complex with the tertiary amine group.
- 8. A protein specific reagent comprising polymeric microspheres covalently bonded to a conjugate of said protein, said microspheres comprising
- polymeric microspheres having a diameter below 100 microns containing at least 20% by weight of a tertiary amine substituted monomer having the amine ligand pendant from the polymer backbone selected from the group consisting of cross-linked polystyrene-divinylbenzene substituted with --CH.sub.2 NMe.sub.2, polyethylene imine, polyvinylbenzimidazole and polyvinylpyridine, said ligands being capable of ionic reaction or coordination with Group IV, VI, VII, or VIII metal compounds selected from oxides, salts or acids to form complexes therewith and said microspheres containing; at least 0.1 weight percent of finely divided, free metal associated with the amine ligands formed by reduction of the compound in situ within the microsphere by reaction with a reducing agent selected from the group consisting of borohydrides and aluminum hydrides and at least 20% by weight of an acrylic monomer having a covalent bonding group selected from hydroxyl, carboxyl or amine.
Parent Case Info
This is a division of application Ser. No. 718,103, filed Aug. 27, 1976, now issued on Oct. 31, 1978, as U.S. Pat. No. 4,123,396.
ORIGIN OF THE INVENTION
The invention described herein was made in the performance of work under a NASA contract and is subject to the provisions of Section 305 of the National Aeronautics and Space Act of 1958, Public Law 83-568 (72 Stat. 435; 42 USC 2457).
US Referenced Citations (7)
Foreign Referenced Citations (1)
Number |
Date |
Country |
1937225 |
Jan 1970 |
DEX |
Divisions (1)
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Number |
Date |
Country |
Parent |
718103 |
Aug 1976 |
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