Patent Application
20170306353
The present invention relates to the field of veterinary vaccines, namely to vaccines for poultry against ND and IBD, based on recombinant herpesvirus of turkeys (HVT) as viral vector. In particular the invention relates to a recombinant DNA expression cassette, to a recombinant HVT comprising the recombinant DNA expression cassette, to a vaccine for poultry based on the recombinant HVT or on host cells comprising the recombinant HVT. Further the invention relates to methods and uses of the expression cassette, the recombinant HVT, and the host cells, for the preparation and use of the vaccine.
Marek's disease (MD) is a highly infectious disease of poultry, occurring worldwide, and is characterized by the presence of T-cell lymphomas in several organs and nerves. This leads to a variety of symptoms, among others paralysis and mortality. New-born chicks can be protected by maternally derived antibodies (MDA) from immune mothers. MD is caused by Marek's disease virus (MDV) which belongs to the family alphaherpesvirideae, and the genus Mardivirus. The virion is enveloped and about 160 nm in size. Within the capsid is comprised a large genome of linear double stranded DNA, between 100 and 200 kbp in size.
HVT replicates in the birds' peripheral blood lymphocytes (PBL's), and is thus a systemic virus which induces an immune response of long duration that is mostly aimed at the cellular immune system.
Next to being used as a vaccine per se, HVT is also used as a viral vector vaccine for the expression and delivery of various immunogenic proteins to poultry, see e.g. WO 87/04463. Typically the expressed gene encodes (a part of) a protective antigen of a micro-organism pathogenic to poultry, against which vaccination is required. Through the years many heterologous genes have been expressed in HVT vectors, such as from: Newcastle disease virus (NDV) (Sondermeijer et al., 1993, Vaccine, vol. 11, p. 349-358), infectious bursal disease virus (IBDV) (Darteil et al., 1995, Virology, vol. 211, p. 481-490), and of a parasite antigen (Cronenberg et al., 1999, Acta Virol., vol. 43, p. 192-197).
The genomic nucleotide sequence of HVT is available, for example from GenBank™ as: AF291866 (strain FC-126). Several methods have been described for inserting heterologous genes into HVT, such as by using homologous recombination (Sondermeijer et al., supra), Cosmid regeneration (US 5,961,982), or Bacmids (bacterial artificial chromosomes) (Baigent et al., 2006, J. of Gen. Virol., vol. 87, p. 769-776).
For the construction of recombinant vectors, the heterologous nucleic acid that is to be inserted into the vector's genome, usually comprises at least one heterologous gene or coding region, which encodes (at least an immunogenic part of) an antigen. Also the construct may comprise a promoter sequence to drive the expression of the heterologous gene, and regulatory signals such as an enhancer, or a transcription terminator. Such a combined insert is often termed an ‘expression cassette’.
Consequently, a recombinant vector vaccine must provide a good replication of the vector and of its insert, both in vitro and in vivo, and an effective expression of the heterologous gene(s) in vivo, preferably of high level, and consistent over time, to induce and maintain a protective immune-response in a target.
Newcastle disease (ND) and infectious bursal disease (IBD) are important diseases of poultry, which occur worldwide, and can cause severe negative effects in the poultry industry regarding animal welfare and economy of operation. This is described e.g. in handbooks, like: The Merck veterinary manual (2010, 10th ed., 2010, C. M. Kahn edt., ISBN: 091191093X), and: ‘Disease of poultry’ (2008, 12th ed., Y. Saif ed., Iowa State Univ. press, ISBN-10: 0813807182).
IBD is caused by infectious bursal disease virus (IBDV), also called ‘Gumboro disease virus’, a member of the Birnaviridae family. These viruses have a genome consisting of two segments (A and B) of double-stranded RNA. The larger segment A encodes a polyprotein of 110 kDa, which is subsequently cleaved by autoproteolysis to form mature viral proteins VP2, VP3 and VP4. Of these, VP2 and VP3 are the structural capsid proteins for the virion, and VP2 is the major host-protective immunogen.
To achieve cost efficiency, a common approach is to design veterinary vaccines that comprise a combination of antigens. In this way a single vaccination round can immunise the animal against a number of diseases at once. Not only does this save time and labour costs, but it also reduces discomfort and stress to the vaccinated animals that would otherwise occur from having to receive repeated vaccinations. This is even more applicable to vaccines that need to be administered by individual injection, such as vaccines based on recombinant HVT as viral vector, therefore combination vaccines in this context are also highly desirable, and the ability to protect against several different diseases at once -in addition to MDV protection from the HVT vector itself—would be a great benefit. However in the past, the mere combination of two separate HVT vectors with single heterologous gene inserts turned out unsuccessful: interference between the replicating vectors caused one or the other to become suppressed in the vaccinated target. Therefore research has focussed on the combined expression and delivery of more than one heterologous antigen from a single recombinant HVT vector.
Several publications describe HVT vector constructs that comprise multi-gene inserts, for example: in WO 93/025.665 and WO 96/005.291, describing bivalent and trivalent ‘vaccines’. Similarly: EP 719.864, and EP 1.026.246. However most of the described multi-gene constructs are only suggested, and only some of the recombinant vectors with multiple inserts were actually constructed and isolated. Very few were ever tested in chickens. Overall no results are given on their stability upon replication, or the expression levels of the foreign genes, let alone any data on the induction of an effective immune protection in target animals.
Unfortunately, upon prolonged testing during product development, one of the main constructs as described in WO 2013/057.235, named HVP309, did not display adequate genetic stability and sustained expression of heterologous inserts. This HVP309 recombinant HVT vector comprises an expression cassette with the NDV F gene, driven by a human cytomegalovirus immediate early 1 gene core promoter, followed downstream by an IBDV VP2 gene that is driven by a chicken beta-actin gene core promoter.
It is an object of the present invention to accommodate to this need in the field, and to provide, for the first time, a recombinant HVT vector vaccine that allows the effective immunisation of poultry against ND and IBD, and that has consistent and reliable genetic stability.
Initially the inventors were disappointed to learn that the HVP309 construct had this inherent genetic instability, leading to loss of expression of its heterologous gene inserts. Without guidance from the prior art on ways to overcome this instability -while maintaining vaccine efficacy and viral replication levels—they had to completely redesign a vector vaccine.
The inventors were therefore surprised to find that one specific recombinant HVT vaccine demonstrated good viral vector replication, a sustained NDV F—and IBDV VP2 gene expression, and effective immunoprotection against ND and IBD, and also has an improved genetic stability over prior art constructs. In fact the stability is now such that no non-expressing virus plaques can be found anymore, even after 15 consecutive passages in cell-culture, and after one passage in birds. In addition, the level of vaccine efficacy against ND and IBDV was slightly (ND) or even considerably (IBDV) improved relative to previous vector constructs.
It is currently not known why the new recombinant HVT vectored ND-IBD vaccine has the improved efficacy and improved stability characteristics.
Therefore in a first aspect the invention relates to a recombinant DNA expression cassette comprising in 5′ to 3′ direction and in this order:
The recombinant DNA expression cassette according to the invention can be used for the generation of a recombinant HVT vector virus vaccine, which is effective in preventing or reducing infection by IBDV, and NDV, or associated signs of disease, and has consistent and reliable genetic stability, both when passaged in vitro and in vivo.
A “recombinant” is a nucleic acid molecule, or a micro-organism of which the genetic material has been modified relative to its starting—or native condition, to result in a genetic make-up that it did not originally possess.
The “expression cassette” for the invention comprises the genes and regulatory elements as described and defined herein. Optionally the expression cassette may also contain other DNA elements that can assist in its generation and manipulation, such as sites for restriction enzyme recognition or PCR primers, to enable molecular cloning. While the expression cassette can exist in DNA or in RNA form, because of its intended use in a HVT vector, therefore the expression cassette is employed as DNA.
The generation, construction and assembly of the recombinant DNA expression cassette according to the invention, and of other genetic elements described herein, can be done by well-known molecular biological techniques, involving cloning, transfection, recombination, selection, and amplification. These, and other techniques are explained in great detail in standard text-books like Sambrook & Russell: “Molecular cloning: a laboratory manual” (2001, Cold Spring Harbour Laboratory Press; ISBN: 0879695773); Ausubel et al., in: Current Protocols in Molecular Biology (J. Wiley and Sons Inc, NY, 2003, ISBN: 047150338X); C. Dieffenbach & G. Dveksler: “PCR primers: a laboratory manual” (CSHL Press, ISBN 0879696540); and “PCR protocols”, by: J. Bartlett and D. Stirling (Humana press, ISBN: 0896036421).
The term “comprising” (as well as variations such as “comprise”, “comprises”, and “comprised”) as used herein, intends to refer to all elements, and in any possible combination conceivable for the invention, that are covered by or included in the text section, paragraph, claim, etc., in which this term is used, even if such elements or combinations are not explicitly recited; and not to the exclusion of any of such element(s) or combinations.
The term “in 5′ to 3′ direction”, also known as: in downstream direction', is well known in the field. Together with the terms “in this order” it serves to indicate the relative orientation which the elements that are summed up thereafter need to have in respect of each other, in order to be functional with the gene-expression machinery of the host cell in which a recombinant HVT comprising the expression cassette according to the invention will be replicated and expressed. As the skilled person will realise, this direction relates to the DNA strand from the double stranded DNA genome of HVT that is the ‘coding strand’, and it relates to the encoded mRNA molecule that is in the ‘+’ or ‘sense’ orientation.
The term “gene” is used to indicate a section of DNA that is capable of encoding a protein. A gene for the invention preferably encodes a complete protein. However, a gene may also encode a section of a protein, for example encoding only the mature form of a protein, i.e. without a ‘leader’, ‘anchor’, or ‘signal sequence’. In that respect gene as used herein corresponds to open reading frame or ORF. A gene may even encode a specific section of a protein, such as the section comprising an immunoprotective epitope.
A “promoter” for the invention is well known to be a functional region on the genome of an organism that directs the transcription of a downstream coding region. A promoter is thus situated upstream of a gene.
Commonly promoters contain a number of recognisable, regulatory regions, such as the enhancer region, which is involved in binding regulatory factors that influence the time, the duration, the conditions, and the level of transcription. While the enhancer region is commonly situated upstream, a promoter also contains a region more downstream that is involved in the binding of transcription factors and directing the RNA polymerase itself. This downstream region generally contains a number of conserved promoter sequence elements such as the TATA box, the CAAT box, and the GC box.
A promoter for the expression of a (heterologous) gene in a (virus) vector needs to be able to effectively drive the transcription of that downstream coding region. This is commonly referred to as the promoter being “operatively linked” to the gene, such that the gene is ‘under the control’ of the promoter, or is ‘driven by’ the promoter. This commonly means that in the expression cassette the promoter and the gene are connected on the same DNA, in effective proximity, and with no signals or sequences between them that would intervene with an effective transcription.
A “transcription terminator” is a regulatory DNA element involved in the termination of the transcription of a coding region into RNA. Commonly such an element encodes a section with a secondary structure, e.g. a hairpin, that can cause the RNA polymerase complex to strop transcription. A transcription terminator is therefore always situated downstream of the stop codon from the region to be translated, the 3′ untranslated region. A terminator can also comprise a poly-adenylation signal, or polyA signal. This induces the polyadenylation that occurs to most eucaryotic mRNA's, and which is relevant for the transportation and stability of mRNA molecules.
For the invention, a gene is “heterologous” to the recombinant HVT vector that carries it, if that gene was not present in the parental HVT that was used to generate the recombinant HVT vector.
The mCMV-IE1—or the hCMV-IE1 gene promoters are well known in the art, and can be readily obtained from a variety of commercial sources, such as from suppliers of commercial plasmids for cloning and expression. The IE1 gene is also called the ‘major IE gene’.
The hCMV-IE1 gene promoter in its complete version is about 1,5 kb in size and consists of an enhancer, a core promoter, and an intron, whereby the promoter activity proceeds into the intron region, see Koedood et al. (1995, J. of Virol., vol. 69, p. 2194-2207).
An “NDV F protein gene” for the invention is well known and sequence information is extensively available in the prior art. The F protein gene can be obtained from a variety of commonly available plasmid constructs. Alternatively, it can be obtained from an NDV isolated from nature, using routine techniques for manipulating an RNA virus. NDV can be readily identified using serology, or molecular biology.
In an embodiment of the expression cassette according to the invention, the mCMV-IE1 gene promoter is a complete promoter, comprising both the core promoter region, as well as the enhancer region for the mCMV-IE1 gene. The complete mCMV-IE1 gene promoter is about 1.4 kb in size.
Similarly, homologs or variants of the promoter element may be used that are equally effective and stable.
In an embodiment, the mCMV-IE1 gene promoter is the region of nucleotides 630-2020 of SEQ ID NO: 1.
In an embodiment of the expression cassette according to the invention, the IBDV VP2 gene for the invention encodes a VP2 protein from an IBDV that is of the classic type. Such genes are well known and their sequence information is readily available in the prior art, see e.g. GenBank acc.nr: D00869 (F52/70), D00499 (STC), or AF499929 (D78). Alternatively, this gene can be obtained from the genome of a classic IBDV isolated from nature, using routine techniques for manipulating a Birnavirus. Classic type IBDV's can be readily identified using serology, or molecular biology.
As homologs or variants of the IBDV VP2 gene may have equal efficacy and stability, therefore in an embodiment, the IBDV VP2 protein gene for the invention has at least 90% nucleotide sequence identity to the full length of the region of nucleotides 2052-3410 of SEQ ID NO: 1. Preferably a nucleotide sequence identity of at least 92, 94, 95, 96, 97, 98, or even 99%, in that order of preference.
In an embodiment the IBDV VP2 protein gene for the invention is derived from the classic IBDV strain Faragher 52/70.
In an embodiment the IBDV VP2 protein gene for the invention is the region of nucleotides 2052-3410 of SEQ ID NO: 1.
For the expression cassette according to the invention, the selection of a specific type of transcription terminator is not critical, as long as effective termination of RNA transcription is provided.
In an embodiment the transcription terminator is derived from simian virus 40 (SV40), preferably from the SV40 late gene. This terminator and its use in heterologous expression, has been applied in molecular virology for many years, and was commercialised by Clontech with their ‘pCMVβ’ cloning plasmids, that are commercially available since the end of the 1980's.
In an embodiment, the transcription terminator is derived from the SV40 late gene and is about 0.2 kb in size.
In an embodiment the transcription terminator derived from the SV40 late gene and about 0.2 kb in size, comprises a nucleotide sequence that has at least 95% nucleotide sequence identity to the full length of the region of nucleotides 3441-3650 of SEQ ID NO: 1. More preferred is a nucleotide sequence identity of at least 96, 97, 98, or even 99%, in that order of preference.
In an embodiment, the transcription terminator from the SV40 late gene is the region of nucleotides 3441-3650 of SEQ ID NO: 1.
In an embodiment of the expression cassette according to the invention, the hCMV-IE1 gene promoter is a core promoter. Such a core promoter will typically be smaller than 1 kb in size; preferably about 0.4 kb in size.
In an embodiment the hCMV-IE1 gene promoter for the invention is a DNA molecule of about 0.4 kb, comprising a nucleotide sequence that has at least 95% nucleotide sequence identity to the full length of the region of nucleotides 3789-4149 of SEQ ID NO: 1. More preferred is a nucleotide sequence identity of at least 96, 97, 98, or even 99%, in that order of preference.
In an embodiment, the hCMV-IE1 gene core promoter is the region of nucleotides 3789-4149 of SEQ ID NO: 1.
In an embodiment of the expression cassette according to the invention, the NDV F protein gene is from an NDV that is of the lentogenic type.
In an embodiment, the NDV F protein gene for the invention has at least 90% nucleotide sequence identity to the full length of the region of nucleotides 4174-5835 of SEQ ID NO: 1. Preferably a nucleotide sequence identity of at least 92, 94, 95, 96, 97, 98, or even 99%, in that order of preference.
In an embodiment the NDV F protein gene for the invention is the region of nucleotides 4174-5835 of SEQ ID NO: 1.
In an embodiment, the expression cassette according to the invention comprises an additional transcription terminator which is located downstream of the NDV F protein gene.
In an embodiment, the additional transcription terminator is derived from the hCMV-IE1 gene. Preferably the additional transcription terminator is about 0.3 kb in size.
In an embodiment the additional transcription terminator derived from the hCMV-IE1 gene, and about 0.3 kb in size, comprises a nucleotide sequence that has at least 95% nucleotide sequence identity to the full length of the region of nucleotides 5847-6127 of SEQ ID NO: 1. More preferred is a nucleotide sequence identity of at least 96, 97, 98, or even 99%, in that order of preference.
In an embodiment, the additional transcription terminator derived from the hCMV-IE1 gene is the region of nucleotides 5847-6127 of SEQ ID NO: 1.
In an embodiment of the recombinant DNA expression cassette according to the invention, one or more or all of the conditions apply selected from the group consisting of:
In an embodiment, the recombinant DNA expression cassette according to the invention comprises 5′ and/or 3′ flanking regions from a gene from HVT. These flanking regions allow for homologous recombination to direct the insertion to a target genetic insertion locus on the vector's genome, and in a desired orientation.
In an embodiment, the recombinant DNA expression cassette according to the invention is about 5.5 kb in size. Because—as described—the exact size is not critical, therefore, in regard to the recombinant DNA expression cassette according to the invention, its size is about 5.5 kb, meaning 5.5 kb ±about 25%, preferably ±about 20, 15, 12, 10, 8, 6, 5, 4, 3, 2, or even ±about 1%, in that order of preference.
As described, homologs or variants of the elements of the recombinant DNA expression cassette according to the invention may be equally safe, stable and effective.
In an embodiment, the recombinant DNA expression cassette according to the invention is the region of nucleotides 630-6127 of SEQ ID NO: 1.
To facilitate the convenient construction, manipulation, and use of the recombinant DNA expression cassette according to the invention, this can itself be comprised in a DNA molecule.
Therefore, in a further aspect, the invention relates to a recombinant DNA molecule comprising the recombinant DNA expression cassette according to the invention.
In an embodiment the recombinant DNA molecule according to the invention consists of a cloning plasmid comprising the recombinant DNA expression cassette according to the invention. Examples of common cloning plasmids are e.g. plasmids from the pBR322, or pUC, series. These are widely commercially available.
When the recombinant DNA molecule according to the invention is to be used in transfection protocols, it is commonly referred to as a ‘transfervector’, ‘shuttle vector’, or ‘donor plasmid’. In this situation the recombinant DNA molecule according to the invention comprises the recombinant DNA expression cassette according to the invention, and the cassette may be flanked on both sides by sequences derived from the vector's genome, to direct the insertion.
In an embodiment the recombinant DNA molecule according to the invention comprises the region of nucleotides 630-6127 of SEQ ID NO: 1.
In an embodiment the recombinant DNA molecule according to the invention comprises the DNA molecule as presented in SEQ ID NO: 1.
The recombinant DNA expression cassette according to the invention is preferably used for the generation of a recombinant HVT vector virus vaccine.
Therefore in a further aspect, the invention relates to a recombinant herpes virus of turkeys (HVT), comprising a recombinant DNA expression cassette according to the invention, whereby the expression cassette is inserted in the Us region of the genome of the recombinant HVT.
The term “herpes virus of turkeys” (HVT) refers to a viral micro-organism that displays the characterising features of its taxonomic group-members such as the morphologic, genomic, and biochemical characteristics, as well as the biological characteristics of that group, such as in their physiologic, immunologic, or pathologic behaviour. This also includes HVT that are sub-classified in any way, for instance as a subspecies, strain, isolate, genotype, variant, subtype, serotype, pathotype, or subgroup and the like.
It will be apparent to a skilled person that the current taxonomic classification of a micro-organism described herein, such as an HVT, MDV, NDV, or IBDV for the invention, could change in time as new insights lead to reclassification into a new or different taxonomic group. However, as this does not change the micro-organism itself or its biological behaviour, but only its scientific name or classification, such re-classified micro-organisms remain within the scope of the invention.
For the invention, “comprising a recombinant DNA expression cassette” relates to the insertion of the recombinant DNA expression cassette according to the invention, into the genome of an HVT. This insertion can in principle be made by any available technique, and can result in an insertion, a substitution or a deletion, relative to the HVT vector's genome, provided the resulting recombinant HVT is able to display its favourable effects of safe, stable and effective multivalent antigen expression. Details and examples are described herein below.
The recombinant HVT according to the invention comprises the recombinant DNA expression cassette according to the invention in a single genetic locus, and in the region of its genome that is known as the
Unique short region, or Us. The Us region of an HVT is well known and readily identifiable; it is situated between two well-known repeated elements in the HVT genome, the IRS and the TRS.
In an embodiment the recombinant HVT according to the invention comprises the recombinant DNA expression cassette according to the invention inserted in the Us2 or in the Us10 gene of the genome of the recombinant HVT.
Particularly stable and effective recombinant HVT vectors for the invention could be made by employing the Us2 gene of the HVT genome as the single genetic insertion locus for the invention.
Therefore, in an embodiment of the recombinant HVT according to the invention, the recombinant DNA expression cassette according to the invention is inserted in the Us2 gene of the genome of the recombinant HVT.
For the invention the terms “in the Us2 gene” or “in the Us10 gene” intend to indicate that an insertion has been made in the region of the HVT genome comprising the Us2 respectively the Us10 gene; this can refer to the gene's promoter or to its coding region. Also, the netto effect of the insertion relative to the HVT genome, may be an insertion, a substitution or a deletion, as described. An expected consequence of such insertion is that the normal coding function of the Us2 resp. Us10 gene is disturbed, or even completely abolished in the resulting recombinant HVT.
In an embodiment the insertion of the recombinant DNA expression cassette in the HVT Us region is an insertion; i.e. apart from a few nucleotides that may be missing or be replaced as a result of the cloning process, no substantial deletion in the Us genome region occurs. Consequently, with the netto increase in size, this way the resulting recombinant HVT has a genome size that is larger than the genome of its parent.
In an embodiment, the recombinant HVT according to the invention comprises a DNA molecule of about 5.5 kb, comprising a nucleotide sequence that has at least 95% nucleotide sequence identity to the full length of the region of nucleotides 630-6127 of SEQ ID NO: 1. More preferred is a nucleotide sequence identity of at least 96, 97, 98, or even 99%, in that order of preference.
In an embodiment, the recombinant HVT according to the invention comprises the region of nucleotides 630-6127 of SEQ ID NO: 1.
In an embodiment the recombinant HVT according to the invention comprises a nucleotide sequence as presented in SEQ ID NO: 1.
To make the recombinant HVT according to the invention safe for vaccine use, the recombinant HVT can be based on a parental HVT that is an established HVT vaccine strain that replicates well, and is known to be suitable for inoculation of young birds or embryos, for example the HVT vaccine strains PB1 or FC-126. These are generally available: FC-126 from ATCC: VR # 584-C, and PB1 is commercially available e.g. from MSD Animal Health. The incorporation of the recombinant DNA expression cassette according to the invention does not increase the virulence or pathogenicity of the parental HVT (on the contrary), and no reversion to virulence is to be expected, as HVT are naturally apathogenic.
Therefore, in an embodiment the parental HVT used for generation of the recombinant HVT according to the invention is an HVT vaccine strain; preferably an HVT vaccine strain of the PB1 or the FC-126 strain.
The recombinant HVT according to the invention is a live recombinant carrier micro-organism, or a “vector” virus, which can advantageously be used for vaccination of poultry. It combines the features of being a safe and effective vaccine against ND and IBD, and in addition is genetically stable.
Being “genetically stable” for the invention means that the genetic make-up of the recombinant HVT according to the invention does not change in subsequent rounds of virus replication, or at least does not change to an extent that would be detectable. In the alternative, unstable constructs can lead to the loss of expression of one or both of the inserted heterologous gene(s). This stability can conveniently be monitored with routine techniques, e.g. by subjecting the recombinant HVT according to the invention to subsequent passaging in cell culture, followed by a passage in animals. Virus re-isolated during these steps, can be plated on cell culture dishes, covered with agar, and incubated until HVT specific plaques become visible; all using routine techniques. Next the plaques can be stained for expression of the F or the VP2 protein using suitable antibody preparations in an immunofluorescence assay (IFA) protocol, and adequate positive and negative controls. The number of plaques that do not show fluorescence can be recorded, whereby at least 100 individual plaques of a particular sample should be monitored.
It was surprisingly found that the recombinant HVT according to the invention in a stringent stability test as described above, maintained the presence and the expression of both NDV F and the IBDV VP2 protein genes, in all of the plaques tested, and for 15 cell-culture passages, as well as for one animal passage. Details are described in the Examples.
The recombinant HVT according to the invention can be amplified by common techniques, mainly by replication in in vitro cultures of primary chicken cells, typically chicken embryo fibroblast cells (CEF's). These can be prepared by trypsinisation of chicken embryos, all well-known in the art. The CEF's are plated in monolayers and infected with the HVT. This process can be scaled up to industrial size production.
Therefore, in a further aspect, the invention relates to a host cell comprising a recombinant HVT according to the invention.
A “host cell” for the invention, is a cell that is susceptible to infection and replication by an HVT. Examples of such cells are avian cells, and in particular lymphocytes, or fibroblasts.
In an embodiment, the host cell according to the invention is a primary avian cell; i.e. a cell that is derived directly from an animal or an animal organ, and not from a cell-line. Typically primary cells can only perform a small and limited number of cell-divisions, whereas cells from a cell-line are effectively immortal, and—under the correct conditions—can keep on dividing.
In an embodiment the primary avian host cell according to the invention is a primary chicken embryo fibroblast (CEF).
In an embodiment, the host cell according to the invention is an immortalised avian cell. Several immortalised avian cells have been described, for example in WO 97/044.443 and WO 98/006.824.
By different methods of cloning and transfection, the recombinant DNA expression cassette according to the invention can be used to obtain the recombinant HVT according to the invention, comprising the expression cassette stably integrated in the Us region of the genome of the recombinant HVT.
Therefore, a further aspect of the invention relates to a method for the construction of a recombinant HVT according to the invention, said method comprising the insertion of a recombinant DNA expression cassette according to the invention, into the Us region of the genome of the recombinant HVT.
The insertion of the recombinant DNA expression cassette according to the invention into an HVT genome to generate the recombinant HVT according to the invention, can be performed in different ways, all known in the art. One convenient way is to use a transfervector; in an embodiment this can be the recombinant DNA molecule according to the invention.
The direct insertion of the recombinant DNA expression cassette according to the invention into an HVT genome is the preferred method to generate a recombinant HVT according to the invention. However there are other well-known ways in which such a recombinant HVT can be generated. For example by indirect insertion, whereby parts of the expression cassette are inserted into HVT in single or in multiple round(s) of transfection. These parts can be devised in such a way that upon integration of all parts, the total insert forms the complete expression cassette, for example by employing overlapping regions to steer the order and the orientation of the parts. An alternative is the use of Bacmids, as described in EP 996.738.
The preferred insertion technique to generate a recombinant HVT according to the invention, is by using cosmid regeneration, e.g. as described in WO 93/25.665. This technique essentially employs a set of large overlapping sub-genomic fragments of the HVT genome to reconstruct a complete HVT genome by cotransfection into host cells. As one of the cosmids is made to comprise the recombinant DNA expression cassette according to the invention, this becomes stably integrated into the genome of the recombinant HVT.
As described, the preferred use of the recombinant HVT according to the invention, is in a vaccine for poultry.
Therefore in a further aspect the invention relates to a vaccine for poultry comprising a recombinant HVT according to the invention, and/or a host cell according to the invention, and a pharmaceutically acceptable carrier.
A “vaccine” is well known to be a composition comprising an immunologically active compound, in a pharmaceutically acceptable carrier. The ‘immunologically active compound’, or ‘antigen’ is a molecule that is recognised by the immune system of the inoculated target and induces an immunological response. The response may originate from the innate or the acquired immune system, and may be of the cellular and/or the humoral type.
The vaccine according to the invention provides a safe and early protection of chickens against ND and IBD. This effect is obtained by preventing or reducing the establishment or the proliferation of a productive infection by a field-infection with NDV or IBDV in their respective target organs. This is achieved for example by reducing the viral load or shortening the duration of the viral replication. In turn this leads to a reduction in the target animal of the number, the intensity, or the severity of lesions and associated clinical signs of disease caused by the viral infection. Such a vaccine is colloquially referred to as a vaccine ‘against’ NDV, or IBDV.
The determination of the effectiveness of a vaccine according to the invention, is well within the skills of the routine practitioner, and can be done for instance by monitoring the immunological response following vaccination or by testing the appearance of clinical symptoms or mortality after a challenge infection, e.g. by monitoring the targets' signs of disease, clinical scores, serological parameters, or by re-isolation of the challenge pathogen, and comparing these results to a vaccination-challenge response seen in mock vaccinated animals. To assess vaccine efficacy against ND, challenge survival is a convenient measurement; for IBD, clinical signs of disease in the bursa can conveniently be used.
Various embodiments, preferences and examples of a vaccine according to the invention will be outlined below.
The term “poultry” for the invention relates to a species of bird of relevance to veterinary practice, and that is susceptible to inoculation with HVT; the preferred poultry species are: chicken, turkey, and quail. Chickens are the most preferred species.
For the invention, the poultry may be of any type, breed, or variety, such as: layers, breeders, broilers, combination breeds, or parental lines of any of such breeds. Preferred types are: broiler, breeder, and layer. Most preferred are broiler chickens, as for this type of birds the early protection against ND and IBD results in the improvement of survival, growth rate and feed conversion.
A “pharmaceutically acceptable carrier” is intended to aid in the stabilisation and administration of the vaccine, while being harmless and well-tolerated by the target. Such a carrier can for instance be sterile water or a sterile physiological salt solution. In a more complex form the carrier can e.g. be a buffer, which can comprise further additives, such as stabilisers or conservatives. Details and examples are for instance described in well-known handbooks such as: “Remington: the science and practice of pharmacy” (2000, Lippincott, USA, ISBN: 683306472), and: “Veterinary vaccinology” (P. Pastoret et al. ed., 1997, Elsevier, Amsterdam, ISBN 0444819681).
The vaccine according to the invention is prepared from a recombinant HVT according to the invention by methods as described herein, which are readily applicable by a person skilled in the art. For example, the recombinant HVT according to the invention is constructed by insertion of a recombinant expression cassette according to the invention by transfection and recombination. Next the desired recombinant HVT is selected, and is amplified industrially in smaller or larger volumes, preferably in in vitro cell cultures, e.g. in CEF's. From such cultures a suspension comprising the virus is harvested, either as whole infected cells or as a cell-free preparation, obtained by cell-disruption. This suspension is formulated into a vaccine and the final product is packaged. Cell-associated vaccine is then stored in liquid nitrogen and freeze-dried vaccine at −20 or at +4° C. After extensive testing for quality, quantity and sterility the vaccine product is released for sale.
In an embodiment the vaccine according to the invention is a cell-associated vaccine.
In an embodiment, the vaccine according to the invention is a cell-free virus vaccine.
“Cell-free” meaning comprising the recombinant HVT according to the invention, and being substantially free of host cells according to the invention. The cell-free vaccine can however contain (very) small amounts of host cell-fragments, remaining from the cell-disruption process. The cell-free vaccine is preferably in freeze dried form. Procedures for freeze-drying are known to persons skilled in the art, and equipment for freeze-drying at different scales is available commercially.
Therefore, in an embodiment, the cell-free virus vaccine according to the invention is in a freeze-dried form.
To reconstitute a freeze-dried vaccine, it is suspended in a physiologically acceptable diluent. This is commonly done immediately before administration, to ascertain the best quality of the vaccine. The diluent can e.g. be sterile water, or a physiological salt solution. The diluent to be used for reconstituting the vaccine can itself contain additional compounds, such as an adjuvant.
In a further embodiment of the freeze dried cell-free vaccine according to the invention, the diluent for the vaccine is supplied separately from the freeze dried cake comprising the active vaccine composition. In this case, the freeze dried vaccine and the diluent composition form a kit of parts that together embody the vaccine according to the invention.
Therefore, in a preferred embodiment of the freeze dried cell-free vaccine according to the invention, the vaccine is a kit of parts with at least two types of containers, one container comprising the freeze dried vaccine, and one container comprising a watery diluent.
The target animal for the vaccine according to the invention can in principle be healthy or diseased, and may be positive or negative for presence of NDV or IBDV, or for antibodies against NDV or IBDV. Also the target can be of any weight, sex, or age at which it is susceptible to the vaccination. However it is evidently favourable to vaccinate healthy, uninfected targets, and to vaccinate as early as possible to prevent any field infection and its consequences.
A vaccine according to the invention can thus be used either as a prophylactic—or as a therapeutic treatment, or both, as it interferes both with the establishment and with the progression of an infection by NDV or IBDV.
In that respect, a further advantageous effect of the reduction of viral load by the vaccine according to the invention, is the prevention or reduction of shedding and thereby the spread of the virus, both vertically to offspring, and horizontally within a flock or population, and within a geographical area. Consequently, the use of a vaccine according to the invention leads to a reduction of the prevalence of NDV or IBDV.
Therefore further aspects of the invention are:
The vaccine according to the invention in principle can be given to target poultry by different routes of application, and at different points in their lifetime, provided the inoculated recombinant HVT can establish a protective infection.
Therefore, in an embodiment, the vaccine according to the invention is administered in ovo.
In an embodiment, the vaccine according to the invention is administered by parenteral route. Preferably by intramuscular—or subcutaneous route.
A vaccine according to the invention can be prepared in a form that is suitable for administration to a poultry target, and that matches with the desired route of application, and with the desired effect.
Depending on the route of application of the vaccine according to the invention, it may be necessary to adapt the vaccine's composition. This is well within the capabilities of a skilled person, and generally involves the fine-tuning of the efficacy or the safety of the vaccine. This can be done by adapting the vaccine dose, quantity, frequency, route, by using the vaccine in another form or formulation, or by adapting the other constituents of the vaccine (e.g. a stabiliser or an adjuvant).
The exact amount of recombinant HVT according to the invention per animal dose of the vaccine according to the invention is not as critical as it would be for an inactivated type vaccine; this because the recombinant HVT can replicate and thus multiply in the target animal up to a level of vireamia that is biologically sustainable. In principle the vaccine dose only needs to be sufficient to initiate such a productive infection. A higher inoculum dose does not shorten the time it takes to reach an optimal vireamic infection in the host. Therefore, very high doses are not effective and in addition are not attractive for economic reasons.
The volume per animal dose of the recombinant HVT according to the invention can be optimised according to the intended route of application: in ovo inoculation is commonly applied with a dose of between about 0,01 and about 0.5 ml/egg, and parenteral injection is commonly done with a dose of between about 0.1 and about 1 ml/bird.
The dosing regimen for applying the vaccine according to the invention to a target organism can be in single or multiple doses, in a manner compatible with the formulation of the vaccine, and in such an amount as will be immunologically effective.
It goes without saying that admixing other compounds, such as stabilisers, carriers, adjuvants, diluents, emulsions, and the like to vaccines according to the invention are also within the scope of the invention. Such additives are described in well-known handbooks such as: “Remington”, and “Veterinary Vaccinology” (both supra).
The vaccine according to the invention effectively is a ‘marker vaccine’ for NDV and for IBDV, because the immunity it generates is only directed against one protein from these viruses. This allows for the “differentiation of infected and vaccinated animals”, the so-called DIVA approach. This can conveniently be detected by a serological assay such as an ELISA or immuno-fluorescence assay.
The vaccine according to the invention already provides multiple immunity: against NDV and IBDV by the expression of the heterologous inserts, and in addition against MDV by the HVT vector itself. Nevertheless it can be advantageous to make further combinations by additional immunoactive components. This can serve to enhance the immune protection already provided, or to expand to other pathogens.
Therefore, in an embodiment, the vaccine according to the invention comprises at least one additional immunoactive component.
Such an “additional immunoactive component” may be an antigen, an immune enhancing substance, a cytokine, a vaccine, or any combination thereof. This provides advantages in terms of cost, efficiency and animal welfare. Alternatively, the vaccine according to the invention, may itself be added to a vaccine.
In an embodiment the at least one additional immunoactive component is an immunostimulatory compound; preferably a cytokine or an immunostimulatory oligodeoxynucleotide.
In an embodiment the at least one additional immunoactive component is an antigen which is derived from a micro-organism pathogenic to poultry. This can be ‘derived’ in any suitable way, for instance as a ‘live’ attenuated, an inactivated, or a subunit antigen from that micro-organism pathogenic to poultry.
The additional antigen derived from a micro-organism pathogenic to poultry, is preferably derived from one or more micro-organism selected from the following groups consisting of:
The additional antigen may be a further HVT-vector vaccine.
In an embodiment of the vaccine according to the invention, the additional antigen derived from a micro-organism pathogenic to poultry is a ‘live’ attenuated MDV, NDV, or IBDV vaccine strain. This serves to improve and expand the immunogenicity of the vaccine according to the invention, and this is advantageous in those cases or geographic areas where very virulent field strains of MDV, NDV or IBDV are prevalent.
Therefore, in an embodiment of the vaccine according to the invention comprising at least one additional immunoactive component, the at least one additional immunoactive component is a micro-organism selected from the group consisting of a vaccine strain from: MDV, NDV and IBDV, or any combination thereof.
Such combination vaccines can be made in a variety of ways: by combining of preparations of virus or host cells, or a mixture of these; all are within the scope of the invention. In a preferred embodiment, the components for such a combination vaccine are conveniently produced separately and then combined by filling into the same vaccine container.
By the methods described above, and exemplified hereinafter, a vaccine according to the invention can be prepared.
Therefore, a further aspect of the invention relates to a method for the preparation of the vaccine according to the invention, said method comprising the steps of:
Suitable host cells and pharmaceutically acceptable carriers for the invention have been described above. Also, suitable methods for infection, culture and harvesting are well known in the art and are described and exemplified herein.
As outlined above in detail, the recombinant HVT according to the invention can advantageously be applied in a vaccine for poultry, providing a safe, stable and effective vaccination against MD, ND and IBD or associated signs of disease, and can be administered to poultry at a very young age.
Therefore, a further aspect of the invention relates to the recombinant HVT according to the invention, for use in a vaccine for poultry.
Consequently, the different aspects and embodiments of the invention can advantageously be used to produce a safe, stable and effective vaccine for poultry.
Therefore, in a further aspect, the invention relates to the use of an expression cassette, a recombinant DNA molecule, a recombinant HVT, or a host cell, all according to the invention, or any combination thereof, for the manufacture of a vaccine for poultry.
As described above, and as exemplified hereinafter, the vaccine according to the invention can advantageously be used to prevent or reduce infection by IBDV and/or NDV, or associated signs of disease, by a single inoculation at very young age.
Therefore further aspects of the invention are:
Details on the use of the vaccine according to the invention, by inoculation of poultry have been described above; specifically the inoculation by intramuscular or subcutaneous inoculation of day old chicks, and the in ovo inoculation of 18 day old embryos.
The invention will now be further described with reference to the following, non-limiting, examples.
In the search for stable and effective recombinant HVT that expressed both NDV F and IBDV VP2, the inventors constructed a series of recombinant HVT constructs with different expression cassettes inserted in Us2, using different elements and orientations. In
In more detail: the IBDV VP2 gene was connected to the following promoters:
Also, the VP2 gene was tested in a native and in a codon optimised version, using the HVT codon table.
All these different recombinant HVT were constructed, transfected, and amplified. Next they were selected by testing for expression in vitro on CEF cells, and for expression and vireamia in vivo by inoculation into experimental animals.
In an animal trial the vireamia and serological responses induced by the various recombinants HVT constructs were tested. Animal experiments were performed essentially as described in WO 2013/057.235. In short: one day-old SPF layer chickens were vaccinated intramuscularly and kept in isolators under negative pressure. At 10, 24 and 38 days post vaccination, HVT virus was re-isolated from spleen (10 and 38 days) or from blood samples (24 days; peripheral blood lymphocytes: PBL), to test vireamia. Serological responses were determined in blood samples taken at 37 days post vaccination. Results are presented in Table 2 and in
All HVT recombinants replicated in the vaccinated chickens. Vireamia levels of HVP364 and its mirrored construct HVP367 were low compared to the other recombinants. A portion of the plaques of both viruses from vireamia at 10 and 24 days, showed no expression of VP2 and/or F in IF-assays. Vireamia levels at 38 days were therefore not performed, and HVP364 and HVP367 were excluded from further studies. All other recombinants showed expression of VP2 and F in all plaques at all-time points tested.
The serological responses induced upon inoculation in experimental animals were determined: for NDV F Elisa values were not discriminatory, therefore heamagglutination (HI) against NDV (Clone 30) was used as a selection tool; for IBDV neutralisation (VN) against D78 was used;
HVP360 is a recombinant HVT according to the invention, and expresses both the IBDV VP2 gene and the NDV F gene. An HVT FC-126 based cosmid set was used to insert its expression cassette in the Us2 gene locus on the HVT genome.
For the construction of HVP360, insertion of the recombinant DNA expression cassette into the HVT Us2 gene locus was performed with a set of overlapping cosmid-derived DNA fragments from HVT vaccine strain FC-126 that, after transfection into CEF, regenerated infectious virus, as described in WO 2013/057.235. Also a pBR322 based plasmid was used as transfer vector. Where possible unique restriction enzyme digestion sites were used; when not available these were introduced by PCR directed insertion of a synthetic linker sequence that comprised such a unique site.
The viral DNA fragments from the cosmid vectors and from the transfer plasmid were excised by digestion with appropriate restriction enzymes. The linear DNA fragments were then transfected into CEF cells by means of calcium phosphate precipitation. After DNA had entered the cell, infectious HVT virus was regenerated by homologous recombination between the overlapping sequences of the DNA fragments, thereby generating an intact HVT FC-126 genome, comprising the expression cassette in Us2. This virus construct was called HVP360.
Progeny of the transfected cultures was amplified once on fresh CEF and checked for the presence of HVT expressing VP2 and F by immunofluorescence assay (IFA) using monoclonal antisera against these antigens. Recombinant virus was isolated by single-plaque purification: monolayers of infected CEF were covered with agarose in culture medium, when HVT CPE was clearly visible. Several plaques were picked randomly and passaged two times on CEF before harvesting and storage as cell associated virus preparation.
Two HVP360 parallel plaque isolates (A1 and B1) were each passaged fifteen times in consecutive CEF cell cultures, and screened for expression of VP2 and F by IFA at different passage levels.
For detailed characterisation of the HVP360 construct, several DNA analyses were performed on plasmid DNA of the transfer vector, and on total DNA of CEF cultures infected with FC-126 or with HVP360 passage 5 from both parallel isolates. Sequence analysis and Southern blot analysis of the coding nucleotide sequence of the inserted cassette and HVT Us2 flanking regions were performed to confirm correct integration into Us2, and genetic stability upon passaging. Southern blot analysis was also performed on the full genome of HVP360 to confirm correct recombination at the overlapping regions of the HVT DNA fragments from the cosmid set that were used to reconstruct the virus.
HVT DNA was isolated from CEF cell cultures infected with HVP360 or control parental HVT FC-126 that had also been assembled from a set of cosmids as used for HVP360 but without Us2 expression cassette; this was used as control virus in subsequent experiments, and called HVT FC-126/435. Virus stocks were passaged once on CEF and total DNA was isolated using the Easy-DNA™ kit (Invitrogen). Plasmid DNA of the transfervector was isolated from E. coli cultures transformed with the transfer vector, and DNA was isolated using the Quantum Prep™ Plasmid Midiprep kit (Bio-Rad).
3.2.3. Characterisation by Southern Blot
Southern blots were performed for an in depth analysis of the genome structure of HVP360 to verify that virus assemblage was exactly as intended and no unintended insertions or deletions had occurred during the virus regeneration.
HVT viral DNA was digested with restriction enzymes PvuI and AatII; or with BamHI, KpnI, Bg/II and EcoRI. Transfer plasmid DNA was digested with restriction enzymes PvuI and AatII.
To detect if any parts of the cloning plasmids had been incorporated in the recombinant HVT, a probe was made by digestion of the plasmid pBR322 into smaller fragments with HaeIII. These fragments were labelled with 32P. All cloning vectors used in HVT reconstruction are derivatives of pBR322, and will be detected by this probe if vector sequences are present. Also, the HVP360 transfer plasmid was used in one lane of the Southern blots as positive control for the detection of plasmid sequences.
To check for correct assembly at the overlapping regions of the cosmid inserts and the repeat regions of the HVT genome, primer pairs were designed to hybridise in these relevant regions and probes were obtained by PCR on HVT FC-126 viral DNA prepared from infected CEF cell cultures. Amplicons were digested into smaller fragments with Sau3AI, labelled with 32P and used as probes in the Southern blot hybridization.
The restriction fragment lengths detected in the Southern blots, were compared to those expected as based on the published sequence for HVT strain FC-126 (GenBank acc. nr. AF291866).
To confirm correct insertion and stability of the coding sequences, a complete DNA sequence analysis was done on the expression cassette and on the HVT Us2 flanking regions.
After transfection, plaque purification and serial passaging, isolates for both parallel isolates of HVP360, from passage levels 5, 10 and 15 were monitored for the maintained expression of the inserted genes by IFA. CEF monolayers were infected with the recombinant isolates, incubated for 2-3 days until CPE was clearly visible, and then fixated with 80% ethanol. Expression of IBDV VP2 or NDV F was detected with monoclonal antibodies as first reagent, and a fluorescein isothiocyanate (FITC) labelled conjugate as secondary antibody, and read by UV microscopy.
Genetic homogeneity and -stability was confirmed by Southern blot analysis using specific probes. Blots hybridized with a plasmid pBR322 probe on lanes containing restricted DNA from strain HVP360 and FC-126, gave no signal with the plasmid probe. However the plasmid probe did react positively with lanes containing restricted DNA from the transfer plasmid, showing fragments specific for the plasmid backbone as predicted. Also, plasmid probe was positive for most bands of the DNA size marker.
The same blot was then hybridized with a probe specific for the Us2 insertion locus, again revealing the restriction fragments as predicted.
Hybridizations showed that the viral genome of HVP360 was reassembled correctly and matched the pattern observed for the parent HVT cosmid-reconstructed strain FC-126/435.
In the hybridizations with probes that detected overlapping sequences and repeat regions, the patterns for HVP360 and FC-126 were found to be largely identical, although in some regions the pattern found differed slightly from the predicted Southern blot hybridization pattern for the junction between unique long and terminal repeat region, based on published DNA sequence for HVT FC-126. However the pattern in these regions is identical for both recombinant—and paternal virus strains.
The entire DNA sequence of inserted cassette and flanking regions of the transfer plasmid used was determined by PCR-sequencing. The consensus sequence of the insert in the viral genome of HVP360 was aligned for both isolates A1 and B1, and compared with the sequence in the transfer plasmid, as well as with the sequence of the insertion region of parent strain FC-126. The result of the alignment confirms that the sequence inserted in HVP360 is identical for isolates A1 and B1.
Plaque purified virus of HVP360 for both parallel isolates, and from all three passage levels 5, 10 and 15, was screened by IFA, for expression of VP2 and F. All plaques tested showed full expression of the F and VP2 genes. This confirmed functional and stable expression of VP2 and F, up to (at least) cell passage level 15.
3.4. Conclusions
HVP360 is a recombinant HVT expressing both IBDV VP2 and NDV F. Detailed characterization by IFA, Southern blot analysis on viral DNA, and DNA sequencing of the insert and flanking regions, confirmed that two independent HVP360 isolates A1 and B1 both had correctly integrated the expression cassette in the Us2 region of HVT FC-126 and functionally express IBDV VP2 and NDV F genes in infected cultures of CEF during 15 subsequent passages in CEF cells after plaque purification.
The expression cassette and total genome of HVP360 isolates A1 and B1 are correct in that no deletions, rearrangements, or additional foreign sequences were detected in restriction digestion patterns obtained after Southern blot hybridization with a series of HVT specific genomic probes. Only one copy of the insert is integrated in the Us2 region.
After detailed sequence analysis of the expression cassettes and the flanking regions of the Us2 insertion locus, it was concluded that HVP360 A1 and B1 are identical, to each other, to the sequence in the transfer vector, and to the parent virus FC-126.
A vaccination-challenge experiment was performed with recombinant HVT HVP360. In short: one day-old SPF layers were vaccinated intramuscularly with either of the parallel isolates HVP360 A1 or B1. At 3 or at 4 weeks post vaccination, the induced protection was measured against a severe challenge infection with either IBDV or NDV: for IBDV challenge strain CS89 was inoculated at 3000 CID50 in 0.1 ml, by ocular route to each eye; for NDV strain Herts 33/56 was given at 6 Log 10 ELD50 in 0.2 ml per animal, by im route. The level of vireamia of the vaccine and of the control virus was determined by virus reisolation from spleens from inoculated animals. Results are presented in Table 3.
The level of challenge protection was determined as follows:
The results showed that in this experiment, im vaccination with HVP360 protected chickens of one day-old against ND at 3 weeks after vaccination, for 59 to 73%. At 4 weeks ND protection was 79 to 93%. Protection against IBD at 3 to 4 weeks after vaccination was 93 to 100%.
In a subsequent vaccination-challenge experiment, using HVP360 as vaccine, and challenging with NDV or with IBDV, the onset of immunity and the duration of immunity were determined. HVP360 vaccine virus was at passage level 13; animals were SPF layers, 1 day old; vaccination route was: subcutaneous; vaccination dose was between 1500 and 2500 pfu/animal dose of 0.2 ml. Challenge virus was either IBDV CS89 or NDV Herts 33/56. Control animals were inoculated sc with HVT FC-126/435.
The results showed that after vaccination with HVP360, more than 90% protection is obtained against challenge with NDV at 4 weeks after vaccination at day old by sc route. This meets the PhEur 0450 monograph requirements for a live ND vaccine.
Even better was the protection achieved against challenge with IBDV: more than 90% protection is obtained against challenge at 2 weeks after vaccination with HVP360. This meets the PhEur 0587 monograph requirements for an IBD vaccine.
Also these results demonstrate that the duration of immuno-protection proceeds until (at least) 8 weeks post vaccination at a level of 100% protection against ND, and against IBD.
In an animal trial, vaccination with different doses of HVP360 was applied, and different routes were tested: in ovo (io) and subcutaneous (sc), to test the response from these doses and these routes against challenge infection with NDV. In addition the vireamia levels of HVP360 vaccine and of FC-126 control virus upon reisolation from inoculated animals in the various treatment groups were determined.
Subcutaneous vaccination appeared to be little dependant on the dose used; doses between 500 and 2500 pfu/animal all reached satisfactory immunoprotection levels.
Ultimately, both routes (io and sc) could raise the same protection at 3 and 4 weeks, of 65-70% and 84-90% respectively.
Schematic view of the insert section of a preferred recombinant DNA molecule according to the invention, comprising the expression cassette and Us2 gene flanking sequences, that was used to generate
HVP360, a preferred recombinant HVT according to the invention. The elements of the expression cassette are drawn to scale.
Abbreviations (from left to right): 5′ US2: flanking upstream sequences from the HVT Us2 gene; mIE: murine CMV IE1 gene promoter; VP2: IBDV VP2 gene; term: SV40 transcription terminator; hIE: human CMV IE1 gene core promoter; F: NDV F gene; term: hCMV IE1 gene terminator; 3′ US2: flanking downstream sequences from the HVT Us2 gene.
Graphic representation (not drawn to scale), of the relevant elements of a representative number of recombinant HVT constructs tested. For comparison the prior art construct HVP309 is also represented.
Vireamia levels of different HVT recombinants at 10, 24 and 38 days post vaccination of one day-old SPF layers, as average per group.
Serological responses induced by the different HVT recombinants, at 37 days post vaccination of one day-old SPF layers, as average per group.
| Number | Date | Country | Kind |
|---|---|---|---|
| 14200340.9 | Dec 2014 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2015/081121 | 12/23/2015 | WO | 00 |
