Patent Application
20230184759
The present invention pertains to the medical field. Particularly, the present invention relates to compounds of general Formula I and to their use as haptens. Moreover, the present invention also refers to conjugates comprising the haptens of the invention and to their use for obtaining antibodies. Finally, the invention also relates to an in vitro method for the detection of infections caused by Pseudomonas aeruginosa by means of the identification and/or quantification of the main signaling molecules from the pqs quorum sensing system (pqs QS system).
Pseudomonas aeruginosa is a gram-negative, ubiquitous bacterium cause of a broad spectrum of human diseases such as pneumonia, septicemia and other life-threatening acute and chronic infections. This bacterium belongs to the group of so-called ESKAPE pathogens, a classification of multidrug resistant “superbugs” based on prevalence, 10-years trend of resistance, transmissibility, treatability and preventability in hospital and community settings. This group of microorganisms represents a serious global health threat for which traditional therapeutic options have become limited. Therefore, there is an increasing urgent need of finding novel strategies to deal with this new generation of resistant pathogens. The correct detection and fast identification of the responsible pathogens in these infections is crucial for an adequate treatment. On the other hand, the lack of diagnostic methods capable of providing reliable and fast results has led to the prescription and misuse of broad-spectra antibiotics, contributing to the generation of resistance.
Current methods are based on culture techniques, which can take up to 72 h in order to obtain conclusive results, commonly triggered by a deficit in both sensitivity and specificity of the technique itself. Molecular detection tools have emerged as an interesting approach to overcome the lack of sensitivity and rapidity of the actual methods. Namely, MALDI-TOF analysis or PCR methods have been successfully developed for detection of different bacteria in either isolates or clinical samples. However, these methods normally require specific equipment, highly qualified personnel, tedious extractions and/or purification steps. Immunochemical-based techniques appear as an interesting alternative and provide great versatility through their application in both optical and electrochemical sensors.
Therefore, there is a need in the state of the art to develop methodologies alternative to those described in the state of the art for the detection of infections caused by Pseudomonas aeruginosa in biological samples, in particular by immunochemical methods.
The present invention is directed to solve this problem by providing for first time, an immunochemical assay for the identification and/or quantification of molecules from pqs quorum sensing system of Pseudomonas aeruginosa, allowing their evaluation as biomarkers of disease for diagnostic purposes.
The present invention relates to compounds of general Formula I and to their use as haptens. Moreover, the present invention also refers to conjugates comprising the haptens of the invention and to their use for obtaining antibodies. On the other hand, the invention also relates to an in vitro method for the detection of infections caused by Pseudomonas aeruginosa by means of the identification and/or quantification of molecules from the pqs QS system, particularly the molecules: 2-heptyl-4-quinolone (HHQ), 2-heptyl-3-hydroxy-4-quinolone (PQS), and/or 2-heptyl-4-quinolone N-oxide (HQNO), using said antibodies and conjugates. The molecules HHQ, PQS and HQNO are characterized by the following formula:
Particularly, the first embodiment of the present invention refers to a compound characterized by the Formula I,
wherein: R1 is selected among H, OH or COO—R; R is selected among H, C1-C4 alkyl or NH2; R2 is selected among C3-C15 alkyl, C3-C15 alkenyl or C3-C15 alkynyl; A is selected among H, COOH, NH2 or SH; R3 is selected among C2-C10 alkyl or (CH2)m—R5; m is a whole number between 1 and 5; R1 is selected among H or OH; and R5 is selected among COOH, SH, NH2, OH or PEG; or any combination thereof. In a particularly preferred embodiment, the compounds characterized by the Formula I are:
The second embodiment of the present invention refers to the use of at least a compound characterized by the Formula I as a hapten. So, the haptens are structurally related to HHQ, PQS and HQNO secreted by the Gram-negative bacteria P. aeruginosa (hereinafter the analytes), for the production of specific antibodies against these analytes. In particular, with the antibodies produced, a diagnostic tool has been developed which allows the detection and/or quantification of HHQ, PQS and HQNO in biological samples of patients who may have these bacteria.
Haptens of general Formula I may be prepared following different methods known by a person skilled in the field of organic synthesis, in particular they may be synthesised following the strategy previously described by Reen et al. (Org Biomol Chem 2012, 10, 8903) but including a functionalized spacer arm susceptible of being conjugated to a carrier protein. In order to maximize the exposure of the most important epitopes, the spacer arm was placed at the C-6 position of the quinolone structure (see Scheme 1)
Therefore, the starting aniline derivative is protected adequately to obtain aniline II. In parallel, Meldrum's acid is made to react with an alkanoyl halide to obtain a derivative III that after methanolysis, gives the desired β-ketoester IV. Afterwards, the condensation reaction between the aniline derivative and β-ketoester is carried out in the presence of a catalytic amount of p-toluene sulfonic acid to obtain enamine V. Finally, a thermic cyclization in diphenyl ether is performed to obtain the desired 4-quinolone structure VI.
To obtain HHQ type haptens an adequate desprotection of the protected functional group at the spacer arm in VI yields haptens Ia derived of HHQ structure.
The synthetic strategy to obtain PQS type haptens (Ib) is carried out following the same procedure as described before for HHQ-derivatives. Once obtained 4-quinolone VI, functionalization of C-3 is carried out by Duff reaction, using hexamine as formyl carbon source using conditions described by Pesci et al. (PNAS 1999, 99, 11229). Transposition to obtain hydroxyl group is performed by Dakin oxidation, which starts with hydrogen peroxide nucleophilic addition followed by hydroxide elimination (Scheme 2).
HQNO type haptens (Ic) can be obtained following the experimental procedure described by Woschek et al. (Synthesis 2007(10): 1517-1522 Woschek, A.; Mahout, M.; Mereiter, K.; Hammerschmidt, F.; Hammerschmidt, F Synthesis of 2-Heptyl-1-hydroxy-4 (1H)-quinolone—Unexpected Rearrangement of 4-(Alkoxycarbonyloxy)quinoline N-Oxides to 1-(Alkoxycarbonyloxy)-4(1H)-quinolones. Synthesis 2007, 2007 (10), 1517-1522.) (Scheme 3). Thus, once obtained 4-quinolone VI, as described before, the high nucleophilicity shown by position C-3 and the carbonyl at C-4 is avoided by blocking the corresponding tautomer through protection reaction. For this purpose, C-4 hydroxyl group can be protected using Boc anhydride or any appropriate protecting group followed by oxidation of nitrogen with m-CPBA to achieve the characteristic N-oxide compound. Finally, the desired hapten Ic is obtained by basic deprotection of functional groups.
The third embodiment of the present invention refers to a conjugate comprising at least a hapten according to Formula I in combination with a second component which confers antigenicity to the conjugate. In a preferred embodiment, the second component is a carrier protein, or a fragment thereof, preferably selected from the group comprising: horseshoe crab hemocyanin (HCH), bovine serum albumine (BSA) or keyhole limpet hemocyanin (KLH). In a preferred embodiment, the conjugate is formed by a covalent bond between the R3 of Formula I and the carrier protein.
In a preferred embodiment the conjugates of the invention are listed in Table 1 below.
The fourth embodiment of the present invention refers to a method for producing a conjugate as defined above, which comprises creating a covalent bond, directly or through a cross-linking agent, between the carrier protein and at least a hapten of Formula I. In a preferred embodiment, the conjugate is formed by a covalent bond between the R3 of Formula I and the carrier protein.
Conjugates may be prepared according to various methods known to anyone skilled in the field of organic and immunochemical synthesis, particularly, general procedures that are shown in the following schemes. Starting materials for preparative methods are commercially available or can be prepared using the methods described in the literature.
In general, in the conjugates of the invention between a hapten and a protein, the proteins are bonded to the hapten covalently by means of the amino acids accessible on their surface, preferably those amino acids with nucleophile-type side chains. The reactive amino acid of the proteins is selected from the list comprising, but without being limited to, cysteine, serine, tyrosine and lysine; it is preferably lysine. The processes to achieve the conjugation of haptens to other carrier molecules depend on the functional group present in the hapten molecule in question. It must also consider the stability and solubility of the hapten. Therefore, given the large variety of haptens that exist, there is no common conjugation method.
In some methods, the hapten and the carrier protein are bound by a cross-linking agent.
For the protein cross-linking, the functional protein groups whereto to the cross-linking agents are targeted comprise amino groups, ε-amino groups of lysine, α-amino terminal groups, cysteine sulfhydryl groups (—SH or thiol groups), carbohydrate groups (in the case of glycoproteins) and carboxyl groups.
Cross-linking agents of proteins through amino groups, lysine ε-amino and terminal α-amino groups include, but without being limited to imidoesters and N-hydroxysuccinimide esters (NETS-esters).
Cross-linking agents of proteins through sulfhydryl groups include, without being limited to, maleimides, haloacetyls (such as iodoacetyl) and pyridyl disulfide (pyridyldithiols).
Cross-linking agents of proteins through carbonyl groups (such as aldehydes or ketones) by oxidative treatment of the glycoprotein carbohydrates include, without being limited to, reagents comprising hydrazides (—NH—NH2-).
Cross-linking agents of proteins through carboxyl groups include, without being limited to, carbodiimides.
Some methods are shown here by way of illustration and not in a limiting sense, since other conjugation methods known by persons skilled in the art may be used.
Haptens with a thiol group can be covalently attached to a carrier protein (SI), which is activated with groups capable of reacting with said thiol group (see Scheme 4) by means of a cross-linking agent such as succinimidyl esters or with any other active ester, having in their structure reactive features with the thiol group and then react with the thiol hapten to obtain the corresponding conjugate.
In the case of haptens with amine and carboxylic groups, they can be conjugated with the carrier protein, among others, using the mixed anhydride method, the carbodiimide method (CDI) or the N-hydroxysuccinimide ester method (NETS) (this latter also known as active ester method).
The fifth embodiment of the present invention refers to the use of a conjugate as defined above for producing antibodies. In a preferred embodiment, the conjugates used as immunogens for the production of antibodies are: I-4, I-6 and I-8.
The sixth embodiment of the present invention refers to an antibody characterized in that it specifically recognizes a conjugate of the invention, or to antiserum comprising said antibody. In a preferred embodiment, the antibodies are, for example, polyclonal antibodies or monoclonal antibodies, intact, or fragments thereof; and includes human, humanized and non-human origin antibodies.
The seventh embodiment of the present invention refers to an in vitro method for detecting and/or quantifying at least a quinolone selected from the group: HHQ, PQS and/or HQNO, in a biological sample, which comprises the use of an antibody or antiserum as defined above.
The antibodies of the invention are valid for their use in any type of immunochemical analysis configuration such as, for example, ELISA-type formats, lateral-flow immunoassay (LFIA,) or of strip, Western-blot, immunoturbidimetry or immunosensors. They are also useful for the preparation of immunoaffinity extraction systems, whether, although not being limited to, immunoaffinity columns or particles biofunctionalized with the antibody, or any other type of support which allows the anchoring of the antibody for the later use of the biohydrid material for the extraction by specific interactions with the antibody.
In a preferred embodiment, the method is carried out by an ELISA assay. The ELISA can be a direct ELISA, indirect ELISA, sandwich ELISA of competitive or non-competitive type. Preferably is a competitive indirect ELISA.
In a preferred embodiment, the method comprises: Immobilizing a conjugate as defined above on a solid support, eliminating the non-immobilized conjugate, adding the sample to be analysed and a first antibody defined above in the solid support of section and incubating, eliminating the first antibody not bound to the conjugate, adding a second antibody conjugated with a detectable labelling agent, said second antibody recognizing the first antibody and incubating, eliminating the second antibody not bound to the first antibody, and detecting and/or quantifying the complex obtained with a composition containing a chromogenic, fluorogenic and/or chemiluminescent indicator substrate. In a preferred embodiment, the sample is obtained from a subject who may have an infection caused by P. aeruginosa. In a preferred embodiment, the sample is selected from the group: sputum, bronchoaspirate (BAS), bronchoalveolar lavage (BAL), blood, serum and/or plasma.
In a preferred embodiment the immobilized conjugates, acting as competitor or coating antigens, are different that those used as immunogens.
In a preferred embodiment the indicator substrate is chromogenic, and the reaction is enzymatic.
The eight embodiment of the present invention refers to a kit for the detection and/or quantification of a quinolone selected from the group: HHQ, PQS and/or HQNO, characterized in that it comprises at least one antibody and a conjugate as defined above.
The ninth embodiment of the present invention refers to a method for treating an infection caused by P. aeruginosa which comprises a previous step wherein the infection is diagnosed following the method of the invention described above.
For the purpose of the present invention the following terms are defined:
The following examples serve to illustrate the invention and should not be considered, in any case, as limiting of the scope thereof.
The chemicals used in the synthesis of the haptens were obtained from Aldrich Chemical Co. (Milwaukee, Wis., USA), Sigma Chemical Co. (St. Louis, Mo., USA) or Acros Organics B.V.B.A. (Morris Plains, N.J., USA). Thin-layer chromatography (TLC) was performed on 0.25 mm, pre-coated silica gel 60 F254 aluminium sheets (Merck, Darmstadt, Germany). 1H and 13C NMR spectra were obtained with a Varian Mercury-400 spectrometer (400 MHz 1H and 101 MHz for 13C). Liquid chromatography/electrospray ionization/mass spectrometry (LC/ESI/MS) was performed in a Waters (Milford, Mass., USA) model composed by an Acquity UPLC system directly interfaced to a Micromass LCT Premier XE MS system equipped with an ESI LockSpray source for monitoring positive and negative ions. Data were processed with MassLynx (V 4.1) software (Waters).
Imidazol (4.7 g, 0.07 mol) was added to a mixture of 2-(4-aminophenyl)ethanol (8.0 g, 0.05 mol) and TBSCl (10.5 g, 0.07 mol) in DMF (115 mL). The reaction was stirred at room temperature for 4 h. Afterwards, water and ethyl acetate were added to the reaction and the mixture was extracted with ethyl acetate 3 times. The combined organic layers were washed with water, brine, dried over MgSO4 and evaporated under reduced pressure. Crude product was purified by silica flash chromatography using as eluent AcOEt/Hexane 9:1. Pure aniline 1 was obtained 11.3 g, yield 90% as orange oil.
1H NMR (400 MHz, CDCl3) δ 6.99 (d, J=8.5 Hz, 2H), 6.62 (d, J=8.5 Hz, 2H), 3.74 (t, J=7.3 Hz, 2H), 2.72 (t, J=7.3 Hz, 2H), 0.88 (s, 9H), 0.00 (s, 6H). 13C NMR (101 MHz, CDCl3) δ 144.64, 130.05, 129.25, 115.28, 65.12, 38.95, 26.11, 18.52, −5.20. HRMS: m/z (ES+) for C14H26NOSi [(M+H)+] calculated 252.1784 found 252.1788 (+1.6 ppm).
Thionyl Chloride (0.75 ml, 0.01 mol) was added to a stirred ice cooled MeOH solution (3 ml). The mixture was left stirring 10 min and 3-(4-aminophenyl) propanoic acid was slowly added (0.5 g, 0,003 mol). The mixture was stirred 16 h under inert atmosphere at room temperature. After completion, the mixture was evaporated under reduced pressure and NaHCO3 std. solution was added. The aqueous phase was extracted 3 times using ethyl acetate. Organic layers were combined, washed with brine, dried over Na2SO4 and evaporated under reduced pressure. The crude product was washed with hexane and evaporated to obtain aniline 2 (0.525 g, 0.0029 mol, yield 97%) as a single product.
1H NMR (400 MHz, CD3OD) δ 6.94 (d, J=8.3 Hz, 2H), 6.66 (d, J=8.3 Hz, 2H), 3.62 (s, 3H), 2.78 (t, J=7.6 Hz, 2H), 2.55 (t, J=7.6 Hz, 2H). 13C NMR (101 MHz, CD3OD) δ 175.37, 146.69, 131.64, 129.88, 116.93, 51.96, 37.12, 31.26. HRMS: m/z (ES+) for C10H13NO2 [(M+H)+] calculated 180.1025 found 180.1028 (+1.7 ppm).
Meldrum's acid (10 g, 0.069 mol) was dissolved in anhydrous DCM (127.6 mL) and cooled to 0° C. under N2 atmosphere. After cooling, pyridine (11.18 mL, 0.14 mol) was added followed by dropwise addition of octanoyl chloride (12.414 g, 0.076 mol). The reaction mixture was stirred 1 h at 0° C. then it was left stirring at RT. Reaction progress was monitored by TLC until completion. Reaction mixture was washed with 5% HCl solution. The organic layer was then washed with distilled water before being dried over anhydrous MgSO4, filtered and concentrated under reduced pressure to yield compound 3 as an orange oil (18.59 g, quantitative yield) which was used in the next step without further purification.
1H NMR (400 MHz, CDCl3) δ 3.06 (t, J=7.8 Hz, 2H), 1.73 (s, 6H), 1.69 (m, 2H), 1.45-1.20 (m, 8H), 0.87 (t, J=6.8 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 198.47, 170.72, 160.33, 104.89, 91.37, 35.89, 31.75, 29.46, 29.03, 26.94, 26.29, 22.72, 14.19. HRMS: m/z (ES−) for C14H21O5 [(M−H)−] calculated 269.1389 found 269.1388 (−0.4 ppm).
5-(1-hydroxyoctylidene)-2,2-dimethyl-1,3-dioxane-4,6-dione 3 (18.5 g, 0.068 mol) was dissolved in MeOH (84 mL) and heated at reflux for 5 h. The reaction was allowed to cool, and the solvent was removed under reduced pressure yielding the crude product as an orange oil. Purification was performed using silica flash chromatography Hex/Et2O 8:2. Compound β-ketoester 4 was obtained as a yellow oil (8.85 g, 0.044 mol, yield 76%).
1H NMR (400 MHz, CDCl3) δ 3.72 (s, 3H, O—CH3), 3.43 (s, 2H, OC—CH2—CO), 2.51 (t, J=7.4 Hz, 2H, CH2), 1.57 (quin., 2H, CH2), 1.34-1.17 (m, 8H, 4×CH2), 0.86 (t, J=6.7 Hz, 3H, CH3). 13C NMR (101 MHz, CDCl3) δ 202.94, 167.80, 52.41, 49.12, 43.19, 31.75, 29.12, 29.07, 23.58, 22.70, 14.16. HRMS: m/z (ES−) for C11H20O3 [(M−H)−] calculated 199.1334 found 119.1331 (−2.5 ppm)
To a solution of compound 4 (7.0 g, 35 mmol) in dry hexane (100 mL) was added aniline 1 (9.7 g, 38 mmol) and p-toluene sulfonic acid (0.12 g, 0.7 mmol). The reaction mixture was heated at reflux under a N2 atmosphere for 16 h. It was allowed to cool down and the reaction mixture was evaporated under reduced pressure, obtaining the crude product 5 (14.5 g, 34 mmol, yield 97%) as an pale orange oil. The product was used in the next step without further purification.
1H NMR (400 MHz, CDCl3) δ 10.22 (s, 1H), 7.16 (d, J=8.3 Hz, 2H), 7.00 (d, J=8.3 Hz, 2H), 4.70 (s, 1H), 3.80 (t, J=6.8 Hz, 2H), 3.68 (s, 3H), 2.79 (t, J=6.8 Hz, 2H), 2.25 (t, J=7.7 Hz, 2H), 1.48-1.35 (m, 2H), 1.38-1.09 (m, 8H), 0.85-0.91 (m, 12H), −0.05 (s, 6H). 13C NMR (101 MHz, CDCl3) δ 171.19, 164.30, 137.34, 136.82, 129.99, 125.31, 84.09, 64.46, 50.37, 39.11, 32.33, 31.74, 29.21, 28.98, 28.16, 26.05, 22.72, 18.47, 14.19, −5.28. HRMS: m/z (ES+) for C25H44NO3Si [(M+H)+] calculated 434.3090 found 434.3087 (−0.7 ppm).
To a solution of compound 4 (1.0 g, 5 mmol) in dry toluene (15 mL) was added aniline 2 (0.98 g, 5.5 mmol) and p-toluene sulfonic acid (0.017 g, 0.1 mmol). The reaction mixture was heated at 85° C. under a N2 atmosphere for 16 h. It was allowed to cool down and the reaction mixture was evaporated under reduced pressure, yielding the crude product as a pale orange oil. The crude product 6 (1.947 g) was used in the next step without further purification (purity by NMR about 70%).
1H NMR (400 MHz, CDCl3) δ 10.22 (s, 1H), δ 7.15 (d, J=8.3 Hz, 2H), 7.00 (d, J=8.3 Hz, 2H), 4.70 (s, 1H), 3.68 (s, 3H), 3.67 (s, 3H), 2.94 (t, J=7.8 Hz, 2H), 2.63 (t, J=7.8 Hz, 2H), 2.25 (t, J=8.0 Hz, 2H), 1.40 (quin., 2H), 1.33-1.13 (m, 8H), 0.84 (t, J=6.9 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 173.38, 171.16, 164.09, 129.07, 128.94, 125.43, 120.49, 84.38, 51.79, 50.39, 35.74, 32.32, 31.70, 30.48, 29.17, 29.14, 28.94, 22.69, 14.17. HRMS: m/z (ES+) for C21H31NO4 [(M+H)+] calcd 362.2331 found 362.2346 (+4.1 ppm).
Compound κ (13.2 g, 30.4 mmol) was added dropwise to refluxing diphenyl ether (250 ml) at 270° C. and maintained for 2 h. Once the reaction cooled to RT, a silica preparative column was used in order to eliminate the diphenyl ether, using hexane as eluent. The crude product was re-absorbed in the same silica using DCM and evaporating under reduced pressure. Afterwards, the product was purified by flash column chromatography using a concentration gradient of eluent from Hexane/AcOEt 4:6 to 3:7. Quinolone 7 was obtained as off-white solid (6.23 g, 15.5 mmol, yield 51%).
1H NMR (400 MHz, CDCl3) δ 11.99 (s, 1H), 8.18 (d, J=1.9 Hz, 1H), 7.65 (d, J=8.5 Hz, 1H), 7.47 (dd, J=8.5, 1.9 Hz, 1H), 6.20 (s, 1H), 3.82 (t, J=7.0 Hz, 2H), 2.91 (t, J=7.0 Hz, 2H), 2.67 (t, J=7.8 Hz, 2H), 1.76-1.64 (m, 2H), 1.38-1.09 (m, 8H), 0.90-0.74 (m, 12H), −0.04 (s, 6H, H17 and H18). 13C NMR (101 MHz, CDCl3) δ 178.89, 154.88, 139.34, 134.92, 133.57, 125.05, 124.93, 118.40, 108.04, 64.56, 39.43, 34.50, 31.80, 29.33, 29.22, 29.13, 26.05, 22.71, 18.43, 14.16, −5.24. HRMS: m/z (ES−) for C24H38NO2Si [(M−H)−] calculated 400.2672 found 400.2678 (+1.5 ppm).
Following an analogous procedure to that described for compound 7, but using ester 6, compound 8 was obtained as a pale-brown solid after purification by flash chromatography column using DCM with 2% MeOH (0.927 g, 2.8 mmol, yield 75%).
1H NMR (400 MHz, CDCl3) δ 11.64 (s, 1H), δ 8.18 (d, J=2.0 Hz, 1H), 7.62 (d, J=8.5 Hz, 1H), 7.45 (dd, J=8.5, 2.0 Hz, 1H), 6.20 (s, 1H), 3.64 (s, 3H), 3.04 (t, J=7.8 Hz, 2H), 2.71-2.62 (m, 4H), 1.76-1.61 (m, 2H), 1.34-1.13 (m, 8H), 0.81 (t, J=6.8 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 178.78, 173.28, 154.74, 139.23, 136.16, 132.70, 125.13, 124.26, 118.65, 108.27, 51.79, 35.73, 34.50, 31.79, 30.73, 29.28, 29.13, 29.11, 22.70, 14.15. HRMS: m/z (ES−) for C20H26NO3 [(M−H)−] calculated 328.1913 found 328.1904 (−2.8 ppm).
To a solution of quinolone 7 (0.15 g, 0.37 mmol) in 5 mL of anhydrous DCM, a solution of boron tribromide in DCM 1M was slowly added under inert atmosphere. The mixture was left under refluxe at 45-50° C. overnight. The reaction mixture was evaporated under reduced pressure. H2O was added and extracted 3 times with AcOEt. Combined organic layers were washed with NaHCO3std, brine, dried over MgSO4 and evaporated under vacuum. The crude product was purified by silica column chromatography using DCM with 2% MeOH as eluent. It was obtained pure compound 9 (0.13 g, 0.36 mmol, yield 96%).
1H NMR (400 MHz, CD3OD) δ 8.07 (d, J=2.0 Hz, 1H), 7.59 (dd, J=8.6, 2.0 Hz, 1H), 7.54 (d, J=8.5 Hz, 1H), 6.21 (s, 1H), 3.67 (t, J=7.2 Hz, 2H), 3.27 (t, J=7.2 Hz, 2H), 2.69 (t, J=7.8 Hz, 2H), 1.74 (p, J=7.4 Hz, 2H), 1.45-1.25 (m, 8H), 0.89 (t, J=6.7 Hz, 3H). 13C NMR (101 MHz, CD3OD) δ 180.40, 156.96, 140.49, 136.57, 134.32, 125.52, 125.45, 119.31, 108.83, 39.78, 34.97, 33.68, 32.85, 30.18, 30.17, 30.08, 23.65, 14.38. HRMS: m/z (ES−) for C18H23BrNO [(M−H)−] calculated 348.0963 found 348.0963 (+0.0 ppm).
A solution of bromoderivative 9 (70 mg, 0.20 mmol) and potassium thioacetate (22.8 mg, 0.20 mmol) in 2.0 mL of anhydrous DMF was stirred during 1 h. The reaction was diluted with AcOEt and washed 3 times with H2O, brine, dried over Na2SO4 and evaporated under reduced pressure to obtain pure compound 10 (69 mg, 0.20 mmol, quantitative yield), used in the next step without further purification.
1H NMR (400 MHz, CD3OD) δ 8.05 (d, J=2.0 Hz, 1H), 7.59 (dd, J=8.6, 2.0 Hz, 1H), 7.53 (d, J=8.5 Hz, 1H), 6.21 (s, 1H), 3.16 (t, J=7.1 Hz, 2H), 2.98 (t, J=7.1 Hz, 2H), 2.70 (t, J=7.6 Hz, 2H), 2.30 (s, 3H), 1.75 (p, J=7.5 Hz, 2H), 1.45-1.26 (m, 8H), 0.89 (t, J=6.9 Hz, 3H). 13C NMR (101 MHz, CD3OD) δ 197.12, 180.40, 156.87, 140.34, 137.50, 134.32, 125.41, 125.24, 119.31, 108.79, 36.49, 34.97, 32.85, 31.25, 30.51, 30.18, 30.08, 23.65, 14.38. HRMS: m/z (ES−) for C20H26NO2S [(M−H)−] calculated 344.1684 found 344.1682 (−0.6 ppm).
Protected thiol 10 (69 mg, 0.2 mmol) and KOH (11.2 mg, 0.2 mmol) were dissolved under inert atmosphere in 1 ml of anhydrous and degassed MeOH. The mixture was stirred during 1 h at RT. The reaction was acidified until pH 6-7 with degassed HCl 1M, diluted with water and extracted 3 times with AcOEt, washed with brine, dried over Na2SO4 and evaporated under reduced pressure to obtain the crude product as a pale yellow solid. Eventually, the crude product was purified by crystallization using hexane/AcOEt to obtain compound I-1 (49 mg, 0.16 mmol, yield 81%).
1H NMR (400 MHz, CDCl3) δ 12.17 (s, 1H), 8.17 (d, J=2.0 Hz, 1H), 7.74 (d, J=8.5 Hz, 1H), 7.44 (dd, J=8.5, 2.0 Hz, 1H), 6.26 (s, 1H), 3.00 (t, J=7.3 Hz, 2H), 2.79 (q, J=7.5 Hz, 2H), 2.71 (t, J=7.8 Hz, 2H), 1.72 (p, J=7.6 Hz, 2H), 1.37-1.12 (m, 8H), 0.80 (t, J=6.8 Hz, 3H).
13C NMR (101 MHz, CDCl3) δ 178.54, 155.37, 139.44, 135.62, 133.00, 124.87, 124.70, 118.89, 108.08, 39.94, 34.49, 31.79, 29.30, 29.23, 29.12, 26.09, 22.71, 14.18. HRMS: m/z (ES−) for C18H24NOS [(M−H)−] calculated 302.1579 found 302.1575 (−1.0 ppm).
Ester 8 (700 mg, 2.1 mmol), hexamine (601.7 mg, 4.3 mmol) and p-TsOH·H2O (453.6 mg, 2.4 mmol, 1.1 equiv) were dissolved in glacial acetic acid (54 ml). The mixture was heated at reflux for 3 h under a nitrogen atmosphere. After cooling, 6 M HCl (13 ml) was added and heating was continued at 115° C. for 1 h. The mixture was allowed to cool, diluted with water, and extracted with ethyl acetate. The combined organic fractions were washed with brine, dried over MgSO4, and concentrated under reduced pressure. Ethanol was used to recover some precipitated product during filtration, evaporated under vacuo and mixed with crude product for purification. The crude was purified by column chromatography on silica flash chromatography using DCM with 6% MeOH and 0.5% glacial acetic acid to obtain acid 11 as an off-white solid (480 mg, 1.4 mmol, yield 66%).
1H NMR (400 MHz, DMSO-d6) δ 12.13 (s, 1H), 10.38 (s, 1H), 7.97 (d, J=2.0 Hz, 1H), 7.62 (dd, J=8.4, 2.0 Hz, 1H), 7.51 (d, J=8.4 Hz, 1H), 3.03 (t, J=7.6 Hz, 2H), 2.94 (t, J=7.4 Hz, 2H), 2.58 (t, J=7.4 Hz, 2H), 1.60 (quin., 2H), 1.41-1.21 (m, 8H), 0.86 (t, J=6.7 Hz, 3H). 13C NMR (101 MHz, DMSO-d6) δ 190.81, 177.98, 173.58, 159.61, 137.98, 137.59, 133.64, 126.12, 123.84, 118.74, 113.23, 48.59, 35.12, 31.54, 31.12, 29.98, 28.98, 28.85, 28.34, 22.04, 13.92. HRMS: m/z (ES−) for C20H24NO4 [(M−H)−] calculated 344.1705 found 344.1708 (+0.9 ppm).
Aqueous hydrogen peroxide (1.05 M, 1.0 ml, 1.0 mmol) and aqueous sodium hydroxide (1.08 M, 1.78 ml, 1.9 mmol) were added to a solution of acid 11 (0.300 g, 0.9 mmol) in ethanol (4.3 ml) under nitrogen atmosphere. The mixture was stirred overnight at room temperature. After completion, the reaction mixture was evaporated under reduced pressure. The crude was purified by flash column chromatography using DCM with 4% MeOH and 0.5% glacial acetic acid. Quinolone 1-2 was obtained (135 mg, 0.4 mmol, yield 47%).
1H NMR (400 MHz, DMSO-d6) δ 11.38 (s, 1H), δ 7.90 (d, J=1.6 Hz, 1H), 7.45 (d, J=8.6 Hz, 1H), 7.43 (dd, J=8.6, 1.6 Hz, 1H), 2.91 (t, J=7.5 Hz, 2H), 2.71 (t, J=7.5 Hz, 2H), 2.57 (t, J=7.5 Hz, 2H), 1.64 (quin., 2H), 1.37-1.18 (m, 8H), 0.84 (t, J=6.6 Hz, 3H). 13C NMR (101 MHz, DMSO-d6) δ 173.69, 168.59, 137.76, 135.95, 135.25, 134.14, 130.86, 122.96, 122.07, 117.86, 35.33, 31.19, 30.04, 28.77, 28.47, 28.12, 27.82, 22.06, 13.93. HRMS: m/z (ES−) for C19H25NO4 [(M−H)−] calculated 330.1705 found 330.1699 (−1.8 ppm).
Ester 8 (129 mg, 0.39 mmol) was dissolved in anhydrous THF (7 ml). Boc2O (94 mg, 0.43 mmol) and a catalytic quantity of 4-(dimethylamino)pyridine (DMAP) (12 mg, 0.1 mmol) were added. Then, the mixture was heated at 60° C. under nitrogen atmosphere during 1 h 30. The mixture was left to cool to RT and concentrated under reduced pressure. The crude was purified by silica flash chromatography using AcOEt/Hex 8:2 as eluent, obtaining Boc protected compound 12 (148 mg, 0.34 mmol, yield 88%).
1H NMR (400 MHz, CDCl3) δ 7.97 (d, J=8.6 Hz, 1H), 7.76 (d, J=2.0 Hz, 1H), 7.55 (dd, J=8.6, 2.0 Hz, 1H), 7.25 (s, 1H), 3.68 (s, 3H), 3.13 (t, J=7.8 Hz, 2H), 2.94 (t, J=7.8 Hz, 2H), 2.72 (t, J=7.8 Hz, 2H), 1.85-1.73 (m, 2H), 1.62 (s, 9H), 1.46-1.22 (m, 8H), 0.87 (t, J=6.8 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 173.25, 163.68, 154.20, 150.66, 148.62, 138.51, 131.15, 129.14, 120.75, 119.74, 112.28, 84.72, 51.84, 39.64, 35.71, 31.90, 31.21, 30.07, 29.64, 29.30, 27.83, 22.77, 14.22. HRMS: m/z (ES+) for C25H36NO5 [(M+H)+] calculated 430.2593 found 430.2579 (−3.3 ppm).
Compound 12 (138 mg, 0.32 mmol) was dissolved in anhydrous DCM (4 ml) and cooled at 4° C. Afterwards, mCPBA (83 mg, 0.48 mmol) was added and the mixture was stirred at 4° C. under nitrogen atmosphere during 4 h. Once the starting material was consumed, more DCM was added, and the solution was washed 3 times with NaHCO3std. The organic phase was concentrated under reduced pressure and it was obtained, without further purification, pure N-oxide 13 (132 mg, 0.30 mmol, yield 92%) as a yellow oil.
1H NMR (400 MHz, CDCl3) δ 8.70 (d, J=9.0 Hz, 1H), 7.77 (d, J=1.9 Hz, 1H), 7.63 (dd, J=9.0, 1.9 Hz, 1H), 7.31 (s, 1H), 3.66 (s, 3H), 3.13 (m, 4H), 2.72 (t, J=7.8 Hz, 2H), 1.88-1.75 (m, 2H), 1.61 (s, 9H), 1.49-1.22 (m, 8H), 0.87 (t, J=6.8 Hz, 3H). 13C NMR (101 MHz, CDCl3) δ 172.93, 150.56, 149.57, 144.13, 141.33, 140.92, 132.08, 122.97, 120.69, 120.59, 113.61, 85.22, 51.90, 35.36, 31.87, 31.81, 30.95, 29.69, 29.19, 27.80, 26.24, 22.76, 14.20. HRMS: m/z (ES+) for C25H36NO6 [(M+H)+] calculated 446.2542 found 446.2539 (−0.7 ppm).
N-oxide 13 (125 mg, 0.28 mmol) was dissolved in a degassed solution of KOH 5 M in EtOH (2.5 ml). The mixture was stirred at RT under nitrogen atmosphere during 1 h. Afterwards, H2O was added, and the mixture was left stirring 30 min. The mixture was acidified with HCl cc until pH=1-2 when a white solid precipitated. It was filtered and dried to obtain the crude product. Quinolone N-oxide 1-3 (72 mg, 0.22 mmol, yield 77%) was obtained after crystallization in EtOH/H2O 4:1.
1H NMR (400 MHz, CD3OD) δ 8.11 (d, J=2.0 Hz, 1H), 8.04 (d, J=8.8 Hz, 1H), 7.73 (dd, J=8.8, 2.0 Hz, 1H), 6.35 (s, 1H), 3.09 (t, J=7.6 Hz, 2H), 2.92 (t, J=7.6 Hz, 2H), 2.70 (t, J=7.6 Hz, 2H), 1.83-1.73 (m, 2H), 1.53-1.24 (m, 8H), 0.91 (t, J=6.9 Hz, 3H). 13C NMR (101 MHz, CD3OD) δ 176.27, 155.85, 140.65, 139.67, 134.59, 125.20, 124.52, 117.17, 107.29, 36.34, 32.88, 32.48, 31.56, 30.43, 30.11, 28.82, 23.69, 14.39. HRMS: m/z (ES−) for C19H2404 [(M−H)−] calculated 330.1705 found 330.1710 (+1.5 ppm).
The reagents used were obtained from Aldrich Chemical Co. (Milwaukee, Wis., USA) and from Sigma Chemical Co. (St. Louis, Mo., USA). Purification of conjugates was carried out in ÄKTA Prime Plus using 2 HiTrap desalting columns both from GE Healthcare (Chicago, Ill., USA) or either by dialysis using Spectra/Por membranes from Spectrumlabs (Piraeus, Greece, EU) with molecular weight cut-off of 12-14 kDa. The matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) was a Bruker autoflex III Smartbeam spectrometer (Billerica, Mass.). The pH and the conductivity of all buffers and solutions were measured with a pH-meter pH 540 GLP and a conductimeter LF 340, respectively (WTW, Weilheim, Germany). Polystyrene microtiter plates were purchased from Nunc (Maxisorp, Roskilde, Denmark). Dilution plates were purchased from Nirco (Barbera del Valles, Spain). Washing steps were performed on a Biotek ELx465 (Biotek Inc.). Absorbances were read on a SpectramaxPlus (Molecular Devices, Sunnyvale, Calif., USA) at a single wavelength mode (450 nm). The competitive curves were analysed with a four-parameter logistic equation using the software GraphPad Prism 7.0 (GraphPad Software Inc., San Diego, Calif., USA).
Unless otherwise indicated, phosphate buffer saline (PBS) corresponds to 10 mM phosphate buffer in 0.8% saline solution (pH 7.5). Coating buffer is a 50 mM bicarbonate-carbonate buffer (pH 9.6). PBST is PBS with 0.05% Tween 20 (pH 7.5). Citrate buffer corresponds to 40 mM sodium citrate (pH 5.5).
The substrate solution contains 0.01% of 3,3′,5,5′-tetramethylbenzidine (TMB) and 0,004% H2O2 prepared in citrate buffer. Borate buffer is 0.2 M sodium borate/boric acid (pH 8.7). All buffers were prepared using ultra-pure Milli-Q® water with a resistivity between 16-18 MΩ cm.
Hapten densities of protein conjugates were estimated by means of MALDI-TOF-MS comparing molecular weight of natural proteins with that of conjugates. MALDI experiments were conducted by deposition of 2 μL of the matrix solution (10 mg mL−1 of sinapinic acid in MeCN/H2O 70:30, 0.1% HCOOH) in the MALDI plate and after drying, 2 μL of the purified sample diluted ½ with MeCN 0.2% HCOOH are added and allowed to dry. Finally, 2 μL of the matrix solution are added over the mixture mentioned above and after drying the resulting spot analyzed by MALDI-TOF. Hapten densities were calculated through the equation: [MW(conjugate)−MW(native protein)]/[MW(hapten)−MW(lost atoms)].
The conjugation procedure was carried out in parallel over 25 mg of HCH (Horseshoe Crab Hemocyanin) or 25 mg of BSA (Bovine Serum Albumine). Each protein was dissolved in 4.5 ml of Borax/Borate buffer pH=8.7. Afterwards 12.5 mg (44 μmol) of succinimidyl iodoacetate (SIA) were dissolved in 1 mL of anhydrous DMF. Over each protein solution, dropwise additions (5×100 μL) of SIA were performed. The reactions were stirred 3.5 h at RT and left overnight at 4° C. without agitation to obtain iodoacetate-BSA and iodoacetate-HCH solutions, which were purified by AKTA using 2 HiTrap desalting column and eluting with Borax/Borate buffer.
8.0 mg (26 μmol) of I-1 hapten were dissolved in 1.7 mL of anhydrous DMF. Afterwards, 850 μL of I-1 solution were added dropwise to each activated protein solution and the mixtures were stirred 4 h at RT and left overnight at 4° C. without agitation. Finally, each immunoreactive agent obtained was purified by dialysis in PBS 0.5 mM (5×5 L) and Milli-Q water (1×5 L) and lyophilized.
The conjugation procedure was carried out in parallel over a solution of 5 mg of KLH (Keyhole Limpet Hemocyanin) or a solution of 5 mg of BSA (Bovine Serum Albumine) in PBS 10 mM.
A solution of 2.62 μL (11 μmop of tri-n-butylamine and 1.56 μL (12 μmop isopropyl cloroformiate was added to a solution of the corresponding hapten, 1-2 or 1-3 to activate the carboxylic acid, (10 μmop dissolved in 400 μL of anhydrous DMF. The mixture was left stirring 15 min at 4° C. and 30 min at RT. Then 200 μL of the reaction mixture were added over each protein solution and the mixture was left 2 h stirring at RT and left overnight at 4° C. without agitation. Each immunoreactive agent obtained was purified by dialysis in PBS 0.5 mM (5×5 L) and Milli-Q water (1×5 L) and lyophilized.
Antibodies were obtained by immunizing female New Zealand white rabbits with the corresponding immunogen, namely 1-4, 1-6 or 1-8.
The protocol used for the production of antibodies was conducted in accordance with the institutional guidelines under a license from the local government (DAAM 7463) and approved by the Institutional Animal Care and Use Committee at the CID-CSIC.
The antisera (As) obtained by immunizing the animals were named as:
The antibody titer was assessed during the immunization process through non-competitive indirect ELISA. Microtiter plates were coated with a fixed concentration of the homologous competitor conjugate (1 mg mL−1) and the avidity of the produced antibodies was measured by preparing serial dilutions of the corresponding As. The animals were exsanguinated after 6 immunizations, and the final blood was collected in vacutainer tubes provided with a serum separation gel. Antisera were obtained by centrifugation at 4° C. for 10 min at 10 000 rpm, then stored at −80° C. in the presence of preservative 0.02% sodium azide.
Non-competitive indirect ELISA were carried out to establish the concentrations of a homologous coating antigen (CA) and As dilutions used in competitive assays. For this purpose, concentrations of BSA conjugates (I-5, I-7, I-9) ranging from 5 μg/ml to 5 ng/ml and As dilutions from 1/1000 to 1/1024000 were assessed. The experimental procedure is fully detailed in the next section. However, in this type of assay no analyte is present, therefore the total volume of As dilution added per well is 100 μL.
Competitive assay conditions were selected at 70% signal saturation giving approximately 1-1.2 units of absorbance.
The competitive assay was carried out in a 96 well Maxisorp flat-bottom plates, coated using 100 μL of a BSA conjugate solution in coating buffer pH=9.6. Then, plates were covered with adhesive plate sealer and incubated overnight at 4° C. The day after, plates were washed with PBST (4×300 μL) using the platewasher Biotek ELx405 HT. Sequentially, 50 μL of the corresponding sample solution, containing the analyte HHQ, PQS or HQNO (2 μM to 0.13 nM) or MH medium were added, followed by 50 μL addition of a fixed As dilution and left without agitation 30 min at RT. After another washing step, a 1/6000 dilution of goat AntiRabbit IgG-HRP in PBST was added and incubated 30 min at RT. After a final washing, 100 μL of a substrate solution was added and left 30 min at RT in the dark. Once the time was consumed, the enzymatic reaction was stopped by adding 50 μl of H2SO4 4M solution and the absorbances read at 450 nm. Absorbance data were plotted and analyzed using GraphPad software. The standard calibration curve was fitted to a four-parameter equation according to the following formula: y=B+(A−B)/[1−(x/C)D], where A is the maximum absorbance, B is the minimum absorbance, C is the concentration producing 50% of the maximal absorbance, and D is the slope at the inflection point of the sigmoid curve. Unless otherwise indicated, the data presented correspond to the average of at least two well replicates.
Performance of the assays was evaluated through the modification of different physicochemical parameters in the competition step. The assessed parameters were: competence time, incubation time, pH, ionic strength, presence of a surfactant (% Tween 20), solubility with addition of organic solvents or cation complexation by EDTA.
Regarding specificity studies, it was followed the experimental procedure for indirect competitive ELISA described above. It was assessed the avidity of As versus other quinolone effector molecules of the pqs system from P. aeruginosa. Each assay was run using HHQ, PQS and HQNO as analyte. Cross reactivity was calculated through the equation: CR (%)=IC50(Cross reactant)/IC50(Analyte)×100.
Culture broth Mueller-Hinton was diluted 1:2, 1:5, 1:10, 1:20 and used to run the standard calibration curve. Subsequently, the dilution providing the best ELISA parameters was selected and the conditions of CA and As dilution adjusted.
Blind spiked samples using diluted MH culture broth were prepared, measured and interpolated in the standard curve mentioned above. The experiment was repeated three different days and the final accuracy results are expressed as mean of all replicates.
The assay was run three different days and three times within the same day.
Clinical Pseudomonas aeruginosa isolates coming from patients diagnosed with acute or chronic respiratory airways infection were grown in MH culture broth 8 h at 37° C. In addition, a reference P. aeruginosa strain (PAO) was also grown under the same conditions. Then, aliquots were taken, centrifuged and analysed using the developed immunochemical assays. The results enclosed in
It has been demonstrated the potential of the immunochemical tools presented here for differentiation of both types of infections. The three studied quinolones are promising biomarkers for diagnostic of infections caused by P. aeruginosa. Moreover, the study of QS could provide much more information about type of infection or disease state.
| Number | Date | Country | Kind |
|---|---|---|---|
| 20382256.4 | Mar 2020 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2021/057909 | 3/26/2021 | WO |
