Claims
- 1. A method for obtaining crystals of a protein from a solution containing more than one protein, said method comprising:
- (a) treating the solution with a solid adsorption material;
- (b) crystallizing the protein from the solution; and
- (c) harvesting the crystals obtained in step (b).
- 2. A method according to claim 14, further comprising removing the solid adsorption material prior to step (b).
- 3. A method according to claim 1, wherein the concentration of protein in the solution is from 0.1 to 25% w/w.
- 4. A method according to claim 3, wherein the concentration of protein in the solution is from 0.5 to 15% w/w.
- 5. A method according to claim 4, wherein the concentration of protein in the solution is from 1 to 10% w/w.
- 6. A method according to claim 1, wherein the solid adsorption material is added to the solution at a concentration of 0.05-5% w/w.
- 7. A method according to claim 6, wherein the concentration of the solid adsorption material is 0.1-2% w/w.
- 8. A method according to claim 1, wherein the solid adsorption material is selected from the group consisting of an activated carbon material, diatomaceous earth, Fuller's earth, bentonite, a hydrophobic resin, an ion-exchange resin, and combinations of any of the foregoing.
- 9. A method according to claim 8, wherein the solid adsorption material is an activated carbon material.
- 10. A method according to claim 1, wherein the protein to be crystallized is an enzyme.
- 11. A method according to claim 10, wherein the enzyme is selected from the group consisting of a protease, a lipase, a cellulase, an amylase, and an oxidoreductase.
- 12. A method according to claim 1, wherein the pH of the protein solution is between 3 and 10.
- 13. A method according to claim 1, further comprising redissolving the crystals after harvest and removing the solid adsorption material.
- 14. A method according to claim 1, wherein the treating step comprises contacting the solution with the solid adsorption material in a column or filter.
- 15. A method according to claim 1, wherein the protein solution is obtained from a fermentation broth.
- 16. A method according to claim 15, wherein, prior to step (a), the fermentation broth is purified by centrifugation, filtration, microfiltration, ultrafiltration, precipitation, evaporation, or a combination thereof.
- 17. A method according to claim 15, wherein, prior to step (a), one or more flocculating agents are added to the fermentation broth.
Priority Claims (1)
Number |
Date |
Country |
Kind |
0295/96 |
Mar 1996 |
DKX |
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CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of PCT/DK97/00095 filed Mar. 3, 1997 which claims priority under 35 U.S.C. 119 of Danish application 0295/96 filed Mar. 14, 1996, the contents of which are fully incorporated herein by reference.
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3966702 |
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Jun 1976 |
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Takechi et al. |
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729208 |
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GBX |
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WOX |
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Non-Patent Literature Citations (2)
Entry |
Ollis et al. (1990) "Protein Crystallization" Methods In Enzymology 182:648-659. |
Judge et al. (1995) "Protein Purification by Bulk Crystallization: The Recovery of Ovalbumin" Biotechnology and Bioengineering 48:316-323. |
Continuations (1)
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Number |
Date |
Country |
Parent |
PCTDK9700095 |
Mar 1997 |
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