The present invention relates to an indole derivative having an EP1 receptor antagonism, which is useful as a pharmaceutical, or a pharmaceutically acceptable salt thereof, a pharmaceutical composition comprising the same, and a pharmaceutical use thereof.
With an increasingly aging and stressed society, the number of patients with lower urinary tract dysfunction (LUTD) has increased. LUTD is a generic term for urine collection disorder and dysuria, and the symptoms derived from LUTD are lower urinary tract symptoms (LUTS). One of the LUTS is an overactive bladder syndrome (OABs). OABs may be generally called overactive bladder (OAB) in some cases. In any case, it is a disease defined as “a symptom syndrome which essentially has urinary urgency and which is usually accompanied by urinary frequency and nocturia. Urge urinary incontinence is not necessary”. The symptoms associated with OABs interfere with general life such as work, daily life, mental activity, and the like, and thus lower the quality of life (QOL). Currently, a first choice drug as an agent for treating OABs is an anticholinergic agent. However, it is necessary for the anticholinergic agent to be used also in due consideration of an anti-muscarinic effect such as thirst and residual urine, and thus, is not always effective for all patients (see, for example, Non-patent literature 1). Under these circumstances, there is a demand for development of a therapeutic agent which has a different mechanism from that of the anticholinergic agent (see, for example, Non-patent literature 1).
Recently, in LUTS, particularly in OABs, the role of urothelium has attracted attention. For LUTS, it has become clear that various chemical mediators are released in the urothelial cells, which cause a micturition reflex through the receptors of bladder sensory nerve terminals. Among them, one of the chemical mediators, prostaglandin E2 (PGE2), binds with a prostaglandin E receptor 1 (EP1 receptor) in the afferent nerves (especially C fibers) in the urothelium to increase the micturition reflex. In addition, PGE2 binds with the EP1 receptors present in the bladder smooth muscle to contract the bladder. In fact, it has been reported that the EP1 receptor antagonists inhibit both of the increase in the micturition reflex and the increase in the afferent nerve activities by PGE2 (see, for example, Non-patent literature 2 and Non-patent literature 3). From these, it is suggested that PGE2 is involved in contraction of the bladder smooth muscle and increase in the bladder sensory nerves through the EP1 receptors. Furthermore, it is reported that EP1 receptor antagonists do not increase the amount of the residual urine, but increase the bladder capacity (see, for example, Non-patent literature 4).
There exist four subtypes, EP2, EP3, and EP4 as well as EP1, of the PGE2 receptor. The EP1 receptor exists in the lungs as well as the bladder and the urothelium, the skeletal muscle, the renal collecting duct, and the like (see, for example, Non-patent literature 2). Therefore, it is expected that by changing the selectivity of the subtypes of the PGE2 receptor, the target organs of the drugs, or the target tissues, a therapeutic agent for desired diseases can be developed.
A compound represented by the general formula (A) is disclosed as a therapeutic drug for Alzheimer's disease (see, for example, Patent literature 1).
[wherein A1 represents -L-CO2H or the like, A2 represents a phenyl group which may have substituents, A3 and A4 independently represent a hydrogen atom, a halogen atom, an alkyl group, an alkoxy group, a haloalkyl group or a haloalkoxy group or the like, L represents —(CH2)n-(CH2)n- or —(CH2)nO(CH2)n- or the like, each n independently represents integer selected from 0 to 8].
However, there is no suggestion or disclosure that these compounds have an EP1 receptor antagonism.
As an indole derivative having an EP1 receptor antagonism, a compound represented by the chemical structural formula (B) (sodium 6-(6-chloro-3-isobutylindol-1-yl)pyridine-2-carboxylate) and a analog thereof are disclosed (see, for example, Non-patent literature 5).
However, these compounds differ from the compounds of the present invention in the chemical structural formula in terms of the position, the type, or the like of a substituent.
The present invention is to provide a compound having an EP1 receptor antagonism or a pharmaceutically acceptable salt thereof, a pharmaceutical composition comprising the same, and a pharmaceutical use thereof.
The present inventors have conducted extensive studies on a compound having an EP1 receptor antagonism, and as a result, they have found that the compounds (I) of the present invention or a pharmaceutically acceptable salt thereof exhibit a potent EP1 receptor antagonism, thereby completing the present invention.
That is, the means for solving the above-described objects are presented below.
[1] A compound represented by the general formula (I) or a pharmaceutically acceptable salt thereof:
[wherein
A represents a group selected from the group consisting of the following a) to h):
one of W1 and W2 represents a nitrogen atom and the other represents ═CH— or a nitrogen atom;
W3 represents an oxygen atom or a sulfur atom;
W4 represents ═CH— or a nitrogen atom;
X represents a hydrogen atom or a halogen atom;
Y1 represents a C1-6 alkylene group;
Y2 represents a single bond or an oxy-C1-6 alkylene group;
R1 represents a hydrogen atom, a C1-6 alkyl group, or a C7-10 aralkyl group;
R2 represents a group selected from the group consisting of the following i) to n):
i) a branched C3-6 alkyl group,
j) a halo-C1-6 alkyl group,
k) a C3-6 cycloalkyl group,
l) a phenyl group, in which the ring is unsubstituted or substituted with 1 to 5 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group,
m) a 6-membered aromatic heterocyclic group, in which the ring is unsubstituted or substituted with 1 to 4 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group, and
n) a 5-membered aromatic heterocyclic group, in which the ring is unsubstituted or substituted with 1 to 3 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group;
R3 represents a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group, a halo-C1-6 alkoxy group, a C1-6 alkylsulfanyl group, a C3-6 cycloalkyl group, a cyano group, an amino group, or a nitro group;
R4 represents a hydrogen atom, a halogen atom, a C1-6 alkyl group, or a C1-6 alkoxy group; and
R5 represents a hydrogen atom, a halogen atom, a C1-6 alkyl group, or a C1-6 alkoxy group;
with the proviso that the bonds with (*) represent binding to Y1, and the bonds with (**) represent binding to Y2].
[2] The compound or a pharmaceutically acceptable salt thereof as set forth in (1), wherein A is a group selected from the group consisting of the following a) to d):
[3] The compound or a pharmaceutically acceptable salt thereof as set forth in (2), wherein A is a group selected from the group consisting of a) to c) below:
[4] The compound or a pharmaceutically acceptable salt thereof as set forth in (3), wherein R2 is a group selected from the group consisting of the following a) to d):
a) a branched C3-6 alkyl group,
b) a C3-6 cycloalkyl group,
c) a phenyl group, and
d) a 5-membered aromatic heterocyclic group or a 6-membered aromatic heterocyclic group;
R4 is a hydrogen atom or a halogen atom; and
R5 is a hydrogen atom.
[5] The compound or a pharmaceutically acceptable salt thereof as set forth in (4), wherein R1 is a hydrogen atom.
[6] The compound or a pharmaceutically acceptable salt thereof as set forth in (5), wherein A is a group selected from the group consisting of a) to e) below:
Y1 is a methylene group and Y2 is a single bond.
[7] The compound or a pharmaceutically acceptable salt thereof as set forth in (6), wherein R3 is a C1-6 alkoxy group.
[8] The compound or a pharmaceutically acceptable salt thereof as set forth in (7), wherein R3 is a methoxy group.
[9] The compound or a pharmaceutically acceptable salt thereof as set forth in (6), wherein R3 is a halogen atom.
[10] The compound or pharmaceutically acceptable salt thereof as set forth in (9), wherein R3 is a fluorine atom.
[11] The compound or pharmaceutically acceptable salt thereof as set forth in (6), wherein R3 is a C1-6 alkyl group.
[12] The compound or pharmaceutically acceptable salt thereof as set forth in (11), wherein R3 is a methyl group.
[13] The compound or pharmaceutically acceptable salt thereof as set forth in (6), wherein R2 is an isopropyl group, an isobutyl group, a sec-butyl group or a 1-ethylpropyl group.
[14] The compound or pharmaceutically acceptable salt thereof as set forth in (6), wherein R2 is a phenyl group or a 5-membered aromatic heterocyclic group.
[15] The compound or pharmaceutically acceptable salt thereof as set forth in (14), wherein R2 is a phenyl group, a 3-thienyl group or a 3-furyl group.
[16] The compound or a pharmaceutically acceptable salt thereof as set forth in (2), wherein A is a group represented by the following formula:
[17] The compound or a pharmaceutically acceptable salt thereof as set forth in (16), wherein R1 is a hydrogen atom.
[18] The compound or a pharmaceutically acceptable salt thereof as set forth in (3), wherein R2 is a group selected from the group consisting of the following a) to c):
W5 is a nitrogen atom or —CR8c═;
R6a, R6b, R6c, R6d and R6e are each independently a group selected from a hydrogen atom, a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group, or a cyano group, with the proviso that all of R6a, R6b, R6c, R6d and R6e are not simultaneously a hydrogen atom;
R7a, R7b and R7c are each independently a hydrogen atom, a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, with the proviso that all of R7a, R7b and R7c are not simultaneously a hydrogen atom; and
R8a, R8b and R8c are each independently a hydrogen atom, a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, with the proviso that all of R8a, R8b and R8c are not simultaneously a hydrogen atom.
[19] The compound or a pharmaceutically acceptable salt thereof as set forth in (18), wherein R1 is a hydrogen atom.
[20] A pharmaceutical composition comprising the compound or a pharmaceutically acceptable salt thereof as set forth in any one of (1) to (19) as an active ingredient.
[21] An EP1 receptor antagonist comprising the compound or a pharmaceutically acceptable salt thereof as set forth in any one of (1) to (19) as an active ingredient.
[22] An agent for treating or preventing lower urinary tract symptoms, comprising the compound or a pharmaceutically acceptable salt thereof as set forth in any one of (1) to (19) as an active ingredient.
[23] A method for preventing or treating lower urinary tract symptoms, comprising administering an effective amount of the compound as set forth in any one of (1) to (19) or a pharmaceutically acceptable salt thereof.
[24] A use of the compound as set forth in any one of (1) to (19) or a pharmaceutically acceptable salt thereof for the manufacture of a pharmaceutical composition for preventing or treating lower urinary tract symptoms.
The compound (I) of the present invention or a pharmaceutically acceptable salt thereof exhibits a potent EP1 receptor antagonism, for example, in a test for confirmation of an EP1 receptor antagonism. Therefore, the compound (I) of the present invention or a pharmaceutically acceptable salt thereof is useful as an agent for treating or preventing lower urinary tract symptoms (LUTS), in particular, overactive bladder syndrome (OABs) or the like, based on its EP1 receptor antagonism.
The terms in the specification are defined.
The “halogen atom” means a fluorine atom, a chlorine atom, a bromine atom, or an iodine atom. In X, a fluorine atom or a chlorine atom is preferable. In R3, a fluorine atom or a chlorine atom is preferable, and a fluorine atom is more preferable.
The “C1-6 alkyl group” means an alkyl group having 1 to 6 carbon atoms, which may be branched. Examples thereof include a methyl group, an ethyl group, a propyl group, an isopropyl group, an n-butyl group, an isobutyl group, a sec-butyl group, a tert-butyl group, an n-pentyl group, an isopentyl group, a neopentyl group, a tert-pentyl group, a 1-methylbutyl group, a 2-methylbutyl group, a 1,2-dimethylpropyl group, an n-hexyl group, an isohexyl group and the like. In R3, a methyl group or an ethyl group is preferable, and a methyl group is more preferable.
The “branched C3-6 alkyl group” means a branched alkyl group having 3 to 6 carbon atoms. Examples thereof include an isopropyl group, an isobutyl group, a sec-butyl group, a tert-butyl group, an isopentyl group, a neopentyl group, a tert-pentyl group, a 1-methylbutyl group, a 2-methylbutyl group, a 1,2-dimethylpropyl group, a 1-ethylpropyl group, an isohexyl group, and the like. It is preferably an isopropyl group, an isobutyl group, a sec-butyl group or a 1-ethylpropyl group. It is more preferably an isopropyl group, a sec-butyl group, or a 1-ethylpropyl group. It is further preferably a sec-butyl group.
The “C1-6 alkoxy group” means an alkoxy group having 1 to 6 carbon atoms, which may be branched. Examples thereof include a methoxy group, an ethoxy group, a propoxy group, an isopropoxy group, a butoxy group, an isobutoxy group, a sec-butoxy group, a tert-butoxy group, a pentyloxy group, a hexyloxy group and the like. In R3, a methoxy group or an ethoxy group is preferable, and a methoxy group is more preferable.
The “halo-C1-6 alkyl group” means a C1-6 alkyl group substituted with the same or different 1 to 5 or 6 halogen atoms. Examples thereof include a monofluoromethyl group, a difluoromethyl group, a trifluoromethyl group, a 2-chloroethyl group, a 2-fluoroethyl group, a 2,2-difluoroethyl group, a 1,1-difluoroethyl group, a 1,2-difluoroethyl group, a 2,2,2-trifluoroethyl group, a 2,2,2,5,5-pentafluoroethyl group, a 2,2,2-trichloroethyl group, a 3-fluoropropyl group, a 2-fluoropropyl group, a 1-fluoropropyl group, a 3,3-difluoropropyl group, a 2,2-difluoropropyl group, a 1,1-difluoropropyl group, a 1-fluorobutyl group, a 1-fluoropentyl group, a 1-fluorohexyl group, a 2,2,2-trifluoro-1-trifluoromethyl-1-ethyl group and the like. It is preferably a monofluoromethyl group, a trifluoromethyl group or a 2-fluoroethyl group.
The “halo-C1-6 alkoxy group” means a C1-6 alkoxy group substituted with the same or different 1 to 5 halogen atoms. Examples thereof include a monofluoromethoxy group, a difluoromethoxy group, a trifluoromethoxy group, a 2-chloroethoxy group, a 2-fluoroethoxy group, a 2,2-difluoroethoxy group, a 1,1-difluoroethoxy group, a 1,2-difluoroethoxy group, a 2,2,2-trifluoroethoxy group, a 2,2,2,5,5-pentafluoroethoxy group, a 2,2,2-trichloroethoxy group, a 3-fluoropropoxy group, a 2-fluoropropoxy group, a 1-fluoropropoxy group, a 3,3-difluoropropoxy group, a 2,2-difluoropropoxy group, a 1,1-difluoropropoxy group, a 4-fluorobutoxy group, a 5-fluoropentyloxy group, a 6-fluorohexyloxy group and the like. It is preferably a monofluoromethoxy group, a difluoromethoxy group or a trifluoromethoxy group.
The “hydroxy-C1-6 alkyl group” means a C1-6 alkyl group substituted with a hydroxy group. Examples thereof include a hydroxymethyl group, a 1-hydroxyethyl group, a 1-hydroxy-1,1-dimethylmethyl group, a 2-hydroxyethyl group, a 2-hydroxy-2-methylpropyl group, a 3-hydroxypropyl group and the like.
The “C1-6 alkylsulfanyl” means a group represented by (C1-6 alkyl)-S—. Examples thereof include a methylsulfanyl group, an ethylsulfanyl group, a propylsulfanyl group, a butylsulfanyl group, a pentylsulfanyl group, a hexylsulfanyl group and the like.
The “C3-6 cycloalkyl group” means a monocyclic saturated alicyclic hydrocarbon group having 3 to 6 carbon atoms. Examples thereof include a cyclopropyl group, a cyclobutyl group, a cyclopentyl group, a cyclohexyl group and the like. In R2, it is preferably a cyclopropyl group or a cyclopentyl group. It is more preferably a cyclopropyl group.
The “C7-10 aralkyl group” means an alkyl group having 1 to 4 carbon atoms, which is substituted with an aryl group. Examples thereof include a benzyl group, a phenethyl group, a 1-phenylethyl group, a 3-phenylpropyl group, a 4-phenylbutyl group and the like.
The “5- or 6-membered aromatic heterocyclic group” means a 5- or 6-membered ring group containing 1 to 4 heteroatoms selected from an oxygen atom, a nitrogen atom, and a sulfur atom in the ring. Examples thereof include a pyridyl group, a pyrimidinyl group, a pyrazinyl group, a pyridazinyl group, a furyl group, a pyrrolyl group, a thienyl group, an imidazolyl group, a pyrazolyl group, a 1,2,4-triazolyl group, an isothiazolyl group, an isoxazolyl group, an oxazolyl group, a thiazolyl group, a 1,3,4-oxadiazolyl group, a 1,2,4-oxadiazolyl group and the like. It is preferably a 5-membered aromatic heterocyclic group, and more preferably a 2-furyl group, a 3-furyl group, a 2-thienyl group or a 3-thienyl group. It is further preferably a 3-furyl group or a 3-thienyl group.
The “5-membered aromatic heterocyclic group” means a 5-membered ring group containing 1 to 4 hetero atoms selected from an oxygen atom, a nitrogen atom, and a sulfur atom in the ring. Examples thereof include a furyl group, pyrrolyl group, a thienyl group, an imidazolyl group, a pyrazolyl group, a 1,2,4-triazolyl group, an isothiazolyl group, an isoxazolyl group, an oxazolyl group, a thiazolyl group, a 1,3,4-oxadiazolyl group, a 1,2,4-oxadiazolyl group and the like. It is preferably a 2-furyl group, a 3-furyl group, a 2-thienyl group or a 3-thienyl group. It is more preferably a 3-furyl group or a 3-thienyl group.
The “6-membered aromatic heterocyclic group” means a 6-membered ring group containing 1 to 4 nitrogen atoms in the ring. Examples thereof include a pyridyl group, a pyrimidinyl group, a pyrazinyl group, a pyridazinyl group and the like. It is preferably a pyridyl group, and more preferably a 3-pyridyl group.
The “C1-6 alkylene group” means a divalent linear or molecular-chained saturated hydrocarbon chain having 1 to 6 carbon atoms. Examples thereof include —CH2—, —CH2CH2—, —CH(CH3)—, —(CH2)3—, —CH(CH3)CH2—, —CH2CH(CH3)—, —CH(CH2CH3)—, —C(CH3)2—, —(CH2)4—, —CH(CH3)—(CH2)2—, —(CH2)2—CH(CH3)—, —CH(CH2CH3)—CH2—, —C(CH3)2CH2—, —CH2—C(CH3)2—, —CH(CH3)—CH(CH3)—, —(CH2)5—, —CH(CH3)—(CH2)3—, —C(CH3)2CH2CH2—, —(CH2)6—, —C(CH3)2(CH2)3— and the like.
The “C1-5 alkylene group” means a divalent linear or molecular-chained saturated hydrocarbon chain having 1 to 5 carbon atoms. Examples thereof include —CH2—, —(CH2)2—, —CH(CH3)—, —(CH2)3—, —CH2—CH(CH3)—, —C(CH3)2—, —(CH2)4—, —(CH2)5— and the like.
The “oxy-C1-6 alkylene group” means —O—CH2—, —O—(CH2)2—, —CH2—O—CH2—, —(CH2)2—O—, —O—CH(CH3)—, —CH(CH3)—O—, —O—(CH2)3—, —(CH2)3—O—, —O—CH(CH3)—CH2—, —CH(CH3)—CH2—O—, —O—C(CH3)2—, —C(CH3)2—O—, —O—(CH2)4—, —O—(CH2)5— or —O—(CH2)6—. It is preferably —O—CH2—, —CH2—O—, —O—(CH2)2—, —O—CH(CH3)—, —O—CH(CH3)—CH2— or —O—C(CH3)2—. It is more preferably —O—CH2—, —O—CH(CH3)— or —O—C(CH3)2—.
Hereinafter, the present invention is described in more detail.
In the case that one or more asymmetric carbon atoms exist in the compound (I) of the present invention, the present invention includes each of compounds in which the respective asymmetric carbon atoms are in an R configuration or S configuration, and compounds having any combination of the configurations. Also, the racemic compound, the racemic mixture, the singular enantiomer, and the diastereomer mixture are encompassed within the scope of the present invention. In the case that geometrical isomerism exists in the compound (I) of the present invention, the present invention includes any of the geometrical isomers.
The compound (I) of the present invention can be converted to a pharmaceutically acceptable salt thereof according to a usual method, as necessary. Such a salt may be presented as an acid addition salt or a salt with a base.
Examples of the acid addition salt include acid addition salts with mineral acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, nitric acid, phosphoric acid and the like, and acid addition salts with organic acids such as formic acid, acetic acid, trifluoroacetic acid, methanesulfonic acid, benzene sulfonic acid, p-toluenesulfonic acid, propionic acid, citric acid, succinic acid, tartaric acid, fumaric acid, butyric acid, oxalic acid, malonic acid, maleic acid, lactic acid, malic acid, carbonic acid, glutamic acid, aspartic acid and the like.
Examples of the salt with a base include salts with inorganic bases, such as a sodium salt, a potassium salt, a calcium salt, a magnesium salt and the like, and salts with organic bases or the like such as piperidine, morpholine, pyrrolidine, arginine, lysine and the like.
In addition, the compound (I) of the present invention or a pharmaceutically acceptable salt thereof also encompasses hydrates, and solvates with pharmaceutically acceptable solvents such as ethanol and the like.
The “EP1 receptor antagonism” as mentioned in the present invention means an action of inhibiting the binding of a prostaglandin E2 (PGE2) to a prostaglandin E receptor 1 (EP1 receptor).
The EP1 receptor antagonism reduces the influx amount of calcium into cells and thus decreased or suppressed the intracellular calcium concentration. As the result, the EP1 receptor antagonism exhibits an action of relaxation of smooth muscles, inhibition of sensory nerve stimulation or the like. Particularly, the EP1 receptor antagonist acts on the bladder, the urothelium or the like, whereby it is useful as an agent for treating or preventing LUTS, in particular, the symptoms of OABs or the like.
Furthermore, the EP1 receptor antagonism can be evaluated based on the efficacy of inhibiting the influx amount of calcium into cells by stimulus of a PGE2 to EP1 receptor. This efficacy can be evaluated by an in vitro test or in vivo test in accordance with “Pharmacological Test Examples” described in JP2008-214224A.
Examples of the preferable substituents for the compound (I) of the present invention or a pharmaceutically acceptable salt thereof are as follows.
(I-1) A is preferably a benzene ring, a pyridine ring, a furan ring or a thiazole ring, and more preferably a benzene ring or a pyridine ring.
(I-2) Y1 is preferably a methylene group, —CH(CH3)—, or —C(CH3)2—, and more preferably a methylene group.
(I-3) Y2 is preferably a single bond or —OCH2—, and more preferably a single bond.
(I-4) R1 is preferably a hydrogen atom or a C1-6 alkyl group, and more preferably a hydrogen atom.
(I-5) R2 is preferably an isopropyl group, an isobutyl group, a sec-butyl group, a 1-ethylpropyl group, a phenyl group, a 5-membered aromatic heterocyclic group, a 6-membered aromatic heterocyclic group, a phenyl group, in which the ring is substituted with 1 to 3 groups independently selected from the group consisting of a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group, a 6-membered aromatic heterocyclic group, in which the ring is substituted with 1 to 2 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group, or a 5-membered aromatic heterocyclic group, in which the ring is substituted with 1 to 2 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group. It is more preferably an isopropyl group, a sec-butyl group, a phenyl group, a 3-furyl group, a 3-thienyl group, or a group selected from the group consisting of the following a) to d):
(wherein
R6a, R6b, R6c, R6d, and R6e are each a group selected from the group consisting of the following e) to g):
e) one group of R6a, R6b, R6c, R6d, and R6e is a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, and the other four groups are hydrogen atoms,
f) two groups of R6a, R6b, R6c, R6d, and R6e are each independently a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, and the other three groups are hydrogen atoms, and
g) three groups of R6a, R6b, R6c, R6d, and R6e are each independently a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, and the other two groups are hydrogen atoms;
R7a, R7b, and R7c are each a group selected from the group consisting of h) and i) below:
h) one group of R7a, R7b, and R7c is a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, and the other two groups are hydrogen atoms, and
i) two groups of R7a, R7b, and R7c are each independently a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, and the other is a hydrogen atom;
for R8a and R8b, when w5 is —CR8c=, one group of R8a, R8b, and R8c is a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, the other two groups are hydrogen atoms, and when w5 is a nitrogen atom, one group of R8a and R8b is a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group or a cyano group, and the other group is a hydrogen atom). It is further preferably a 3-furyl group, a 3-thienyl group or a phenyl group.
(I-6) R3 is preferably a fluorine atom, a chlorine atom, a methyl group, an ethyl group, a trifluoromethyl group, a methoxy group, an ethoxy group or a difluoromethoxy group, more preferably a fluorine atom, a chlorine atom, a methyl group, a methoxy group, an ethoxy group, or a difluoromethoxy group, further preferably a fluorine atom or a methoxy group, and particularly preferably a methoxy group.
(I-7) R4 is preferably a hydrogen atom, a fluorine atom or a chlorine atom, and more preferably a hydrogen atom.
A preferable embodiment of the compound (I) of the present invention or a pharmaceutically acceptable salt thereof is a compound formed by combinations of preferable substituents described in (I-1) to (I-7).
A preferable embodiment of the present invention is as follows:
A is a following:
X is a hydrogen atom, a fluorine atom or a chlorine atom;
Y1 is a methylene group;
Y2 is a single bond or —O—CH2—;
R1 is a hydrogen atom;
R2 is a phenyl group or a 5-membered aromatic heterocyclic group;
R3 is a fluorine atom, a methyl group, a methoxy group or an ethoxy group; and
R4 is a hydrogen atom, a fluorine atom or a chlorine atom.
Examples of the concrete compound included in the present embodiment include the following compounds:
Another preferable embodiment of the present invention is as follows:
A is a following:
X is a hydrogen atom, a fluorine atom or a chlorine atom;
Y1 is a methylene group;
Y2 is a single bond or —O—CH2—;
R1 is a hydrogen atom;
R2 is an isopropyl group or a sec-butyl group;
R3 is a fluorine atom, a methyl group, a methoxy group or an ethoxy group; and
R4 is a hydrogen atom, a fluorine atom or a chlorine atom.
Examples of the concrete compound included in the present embodiment include the following compounds:
Another preferable embodiment of the present invention is as follows:
R1 is a C1-6 alkyl group.
Another preferable embodiment of the present invention is as follows:
A is a following:
X is a hydrogen atom, a fluorine atom or a chlorine atom;
Y1 is a methylene group;
Y2 is a single bond or —O—CH2—;
R1 is a C1-6 alkyl group;
R2 is an isopropyl group, a sec-butyl group, a phenyl group or a 5-membered aromatic heterocyclic group;
R3 is a fluorine atom, a chlorine atom, a methyl group, a methoxy group or an ethoxy group; and
R4 is a hydrogen atom, a fluorine atom or a chlorine atom.
Another preferable embodiment of the present invention is as follows:
A is a following:
X is a hydrogen atom, a fluorine atom or a chlorine atom;
Y1 is a methylene group;
Y2 is a single bond or —O—CH2—;
R1 is a hydrogen atom;
R2 is a 6-membered aromatic heterocyclic group;
R3 is a fluorine atom, a methyl group, a methoxy group or an ethoxy group; and
R4 is a hydrogen atom, a fluorine atom or a chlorine atom.
Examples of the concrete compound included in the present embodiment include the following compounds:
Another preferable embodiment of the present invention is as follows:
A is a following:
Y1 is a methylene group;
Y2 is a single bond;
R1 is a hydrogen atom;
R2 is a phenyl group, which may have a substituent, or a 5-membered aromatic heterocyclic group, which may have a substituent;
R3 is a fluorine atom, a methyl group, a methoxy group or an ethoxy group; and
R4 is a hydrogen atom, a fluorine atom, a chlorine atom or a methyl group.
Examples of the concrete compound included in the present embodiment include the following compounds:
Production Process of Compound (1) of the Present Invention
The compound (I) of the present invention or a pharmaceutically acceptable salt thereof can be prepared by a method described in the following Schemes 1 to 3, or a similar method thereto, or by a method described in other literature, or a similar method thereto.
[A] Synthesis of Compounds (Ia) to (Id)
The compound (I) of the present invention can be prepared by the method shown in Schemes 1 or 2 as compounds (Ia) to (Id). Further, when a protecting group is needed, combinations of introduction and cleavage can appropriately be carried out according to a usual method.
In the compound (I) of the present invention, the compound (Ia) wherein R1 is a C1-6 alkyl group or a C7-10 aralkyl group, and the compound (Ib) wherein R1 is a hydrogen atom can be prepared by each method shown in Scheme 1 or a similar method thereto, or be prepared according to a method described in other literature or a similar method thereto. Further, when a protective group is needed, combinations of introduction and cleavage can appropriately be carried out according to a usual method.
(wherein A, R2, R3, R4, R5, Y1 and Y2 have the same meanings as defined above; Ra is a C1-6 alkyl group or a C7-10 aralkyl group; and L1 represents a chlorine atom, a bromine atom, an iodine atom, a methanesulfonyloxy group or the like).
Step 1-1
A compound (Ia) can be prepared by reacting a compound (1) with a compound (2) in a solvent in the presence of a base. As the solvent to be used, N,N-dimethylformamide, N,N-dimethylacetamide, N,N-dimethylimidazolidinone, tetrahydrofuran, a mixed solvent thereof and the like can be illustrated. As the base to be used, sodium hydride, potassium tert-butoxide, lithium bis(trimethylsilyl)amide and the like can be illustrated. The reaction temperature is usually −20° C. to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 3 days. Further, the present step can be carried out with addition of sodium iodide, tetra-n-butyl ammonium bromide, tetra-n-butyl ammonium iodide or the like, if necessary.
Furthermore, the compound (2) used in the present step may be commercially available. Further the compound (2) can be obtained by using the corresponding alcohol as a starting material and converting the hydroxy group into a chlorine atom, a bromine atom, an iodine atom, a methanesulfonyloxy group or the like according to a method described in literature or a similar method thereto.
Step 1-2
A compound (Ib) of the present invention can be prepared by treating the compound (Ia) of the present invention according to a conversion method from a ester group to a carboxy group. Such method is well-known to a skilled person in the art, and can be carried out using the method described in “Greene's Protective Groups in Organic Synthesis”, edited by Greene & Wuts, fourth edition, Wiley-Interscience, 2006.
In the compound (I) of the present invention, the compounds (Ic) and (Id), wherein R2 is a phenyl group, a 5 or 6-membered aromatic heterocyclic group, a phenyl group, in which the ring is substituted with 1 to 5 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group, a 6-membered aromatic heterocyclic group, in which the ring is substituted with 1 to 4 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group, or a 5-membered aromatic heterocyclic group, in which the ring is substituted with 1 to 3 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group can be prepared by the method shown in Scheme 2 or a similar method thereto, or can be prepared according to a method described in other literature or a similar method thereto. Further, when a protective group is needed, combinations of introduction and cleavage can appropriately be carried out according to a usual method.
(wherein A, R3, R4, R5, Ra, Y1 and Y2 have the same meanings as defined above; Rb is a phenyl group, a 5 or 6-membered aromatic heterocyclic group, a phenyl group, in which the ring is substituted with 1 to 5 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group, a 6-membered aromatic heterocyclic group, in which the ring is substituted with 1 to 4 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group, or a 5-membered aromatic heterocyclic group, in which the ring is substituted with 1 to 3 groups independently selected from the group consisting of: a halogen atom, a C1-6 alkyl group, a halo-C1-6 alkyl group, a hydroxy-C1-6 alkyl group, a C1-6 alkoxy group and a cyano group; and Y3 represents a single bond or a C1-5 alkylene group).
Step 2-1
A compound (4) can be prepared by reacting a compound (3) with carbon tetrabromide and triphenylphosphine in a solvent. As the solvent to be used, dichloromethane, 1,2-dichloroethane, benzene, toluene, tetrahydrofuran, a mixed solvent thereof, and the like can be illustrated. The reaction temperature is usually from −20° C. to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 3 days. Furthermore, the compound (3) used in the present step may be commercially available or can be prepared according to a method described in other literature or a similar method thereto.
Step 2-2
A compound (5) can be prepared by reducing a nitro group of the compound (4) using a reducing agent. As the reduction method of a nitro group, for example, a method using iron, zinc, tin(II) chloride dihydrate or the like as a reducing agent in a solvent can be illustrated. As the solvent to be used, methanol, ethanol, acetic acid, water, a mixed solvent thereof and the like can be illustrated. The reaction temperature is usually from −20° C. to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 3 days.
Step 2-3
A compound (7) can be prepared by a reductive amination reaction using the compound (5) and the compound (6). As the solvent to be used, tetrahydrofuran, dichloromethane, 1,2-dichloroethane, ethanol, a mixed solvent thereof and the like can be illustrated. As the reducing agent to be used, sodium triacetoxyborohydride, sodium cyanoborohydride and the like can be, illustrated. The reaction temperature is usually from −20° C. to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 3 days. Furthermore, the compound (6) used in the present step may be commercially available or can be prepared according to a method described in other literature or a similar method thereto.
Step 2-4
A compound (Ic) of the present invention can be prepared by reacting the compound (7) with a compound (8) in a solvent in the presence of a palladium catalyst and a base. As the solvent to be used, toluene, tetrahydrofuran, 1,4-dioxane, ethanol, N,N-dimethylformamide, water, a mixed solvent thereof and the like can be illustrated. As the palladium catalyst to be used, palladium (II) acetate, bis(triphenylphosphine)palladium (II) dichloride, tetrakis(triphenylphosphine)palladium (0), tris(dibenzylideneacetone) dipalladium (0) and the like can be illustrated. As the base to be used, potassium phosphate, potassium phosphate monohydrate, potassium carbonate, cesium carbonate, cesium fluoride, sodium carbonate and the like can be illustrated. The reaction temperature is usually from room temperature to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 3 days. Further, the present step may be carried out with addition of a ligand such as 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl, bis(diphenylphosphino)ferrocene or the like, if necessary. Furthermore, the compound (8) used in the present step may be commercially available or can be prepared according to a method described in other literature or a similar method thereto.
Step 2-5
The compound (Id) of the present invention can be prepared by treating the compound (Ic) of the present invention according to a method of Step 1-2.
The compound (1) may be commercially available, or can be prepared by a method described in the following Scheme 3 or a similar method thereto, or a method described in other literature or a similar method thereto. Further, when a protective group is needed, combinations of introduction and cleavage can appropriately be carried out according to a usual method.
(wherein R2, R3, R4 and R5 have the same meanings as defined above)
Step 3-1
A compound (11) can be prepared by lithiating a compound (9) in a solvent using alkyllithium or the like and then reacting a compound (10) thereto. As the solvent to be used, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, 1,4-dioxane, a mixed solvent thereof and the like can be illustrated. As the alkyllithium to be used, n-butyllithium, sec-butyllithium, tert-butyllithium and the like can be illustrated, and sec-butyllithium is preferable. The reaction temperature is usually from −78° C. to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 1 day. Furthermore, the compounds (9) and (10) used in the present step may be commercially available or can be prepared according to a method described in other literature or a similar method thereto.
Step 3-2
The compound (1) can be prepared by treating the compound (11) with acid in a solvent. As the solvent to be used, dichloromethane, chloroform, methanol, ethanol, tetrahydrofuran, 1,4-dioxane, a mixed solvent thereof and the like can be illustrated. As the acid to be used, trifluoroacetic acid, methanesulfonic acid, concentrated hydrochloric acid, concentrated sulfuric acid and the like can be illustrated. The reaction temperature is usually from −78° C. to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 3 days.
Step 3-3
A compound (13) can be prepared by lithiating the compound (9) in a solvent using alkyllithium or the like and then reacting a compound (12) thereto. As the solvent to be used, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, 1,4-dioxane, a mixed solvent thereof and the like can be illustrated. As the alkyllithium to be used, n-butyllithium, sec-butyllithium, tert-butyllithium and the like can be illustrated, and sec-butyllithium is preferable. The reaction temperature is usually from −78° C. to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 1 day. Furthermore, the compound (12) used in the present step may be commercially available or can be prepared according to a method described in other literature or a similar method thereto.
Step 3-4
A compound (14) can be prepared by treating the compound (13) under acidic condition. This reaction is well-known to a skilled person in the art and can be carried out using the method described in “Greene's Protective Groups in Organic Synthesis” edited by Greene & Wuts, fourth edition, Wiley-Interscience, 2006.
Step 3-5
The compound (1) can be prepared by oxidizing the compound (14) in a solvent in the presence of a palladium catalyst, a oxidizing agent and a base. As the solvent to be used, N,N-dimethylformamide, 1-methyl-2-pyrrolidone, a mixed solvent thereof and the like can be illustrated. As the palladium catalyst to be used, for example, tetrakis(triphenylphosphine)palladium (0) can be illustrated. As the oxidizing agent to be used, for example, mesityl bromide can be illustrated. As the base to be used, potassium carbonate, cesium carbonate, sodium hydride and the like can be illustrated. The reaction temperature is usually from room temperature to a solvent reflux temperature, and the reaction time varies depending on a starting material and a solvent to be used, a reaction temperature or the like, but it is usually 30 minutes to 3 days.
These schemes shown above are exemplification of the method for preparing the compound (I) of the present invention or an intermediate for preparation. These are allowed to be modified to such a scheme that can be readily understood by a person skilled in the art.
Also, in the case that there is a need of a protective group according to the kind of the functional group, combinations of introduction and cleavage can be appropriately carried out according to a usual method. The type, introduction, and cleavage of the protective group can be illustrated in reference to the method described in, for example, “Greene's Protective Groups in Organic Synthesis”, edited by Theodra W. Greene & Peter G. M. Wuts, fourth edition, Wiley-Interscience, 2006.
The intermediates used for preparation of the compound (I) of the present invention or a pharmaceutically acceptable salt thereof can be isolated/purified, as necessary, by solvent extraction, crystallization/recrystallization, chromatography, preparative high performance liquid chromatography, or the like, that is an isolation/purification means well-known to a skilled person in the art.
Pharmaceutical Composition Comprising Compound (I) of the Present Invention or Pharmaceutically Acceptable Salt Thereof
The pharmaceutical composition comprising the compound (I) of the present invention or a pharmaceutically acceptable salt thereof as an active ingredient is used in various dosage forms according to the usages. Examples of the dosage forms include powders, granules, fine granules, dry syrups, tablets, capsules, injections, liquids, ointments, suppositories, plasters, sublinguals, and the like, which are administered orally or parenterally.
These pharmaceutical compositions can be prepared by appropriately mixing or diluting/dissolving with pharmaceutical additives such as an excipient, a disintegrant, a binder, a lubricant, a diluent, a buffering agent, a tonicity agent, a preservative, a wetting agent, an emulsifier, a dispersant, a stabilizer, a solubilizing aid, and the like by a well-known method according to the dosage forms. In addition, when used in combination with agents other than the EP1 receptor antagonist, the pharmaceutical compositions can be prepared by formulating the respective active ingredients simultaneously or separately as described above.
Pharmaceutical Use of Compound (I) of the Present Invention or Pharmaceutically Acceptable Salt Thereof
The compound (I) of the present invention or a pharmaceutically acceptable salt thereof exhibits a potent EP1 receptor antagonism in a test for confirmation of an EP1 receptor antagonism. Therefore, the compound (I) of the present invention can suppress or decrease the intracellular calcium concentration. Accordingly, a pharmaceutical composition comprising the compound (I) of the present invention or a pharmaceutically acceptable salt thereof as an active ingredient can be used as an agent for treating or preventing diseases or symptoms caused by activation of the EP1 receptor due to stimulus of a PGE2.
In addition, examples of the diseases with the activation of the EP1 receptor due to the PGE2 stimulus include lower urinary tract symptoms (LUTS), inflammatory diseases, pain diseases, osteoporosis, cancer, and the like. The pharmaceutical composition comprising the compound (I) of the present invention or a pharmaceutically acceptable salt thereof as an active ingredient is preferably used as an agent for treating or preventing LUTS, inflammatory diseases, or pain diseases. It is more preferably LUTS.
Examples of the disease that causes the lower urinary tract symptoms include overactive bladder (OAB), benign prostatic hyperplasia (BPH), cystitis such as interstitial cystitis and the like, prostatitis, and the like.
The “lower urinary tract symptoms” means storage symptoms, voiding symptoms, post micturition symptoms, or the like. The compound (I) of the present invention or a pharmaceutically acceptable salt thereof is preferably used for treatment or prevention of storage symptoms.
Examples of the “storage symptoms” include urinary urgency, increased daytime frequency, nocturia, urinary incontinence (stress urinary incontinence, urge urinary incontinence, mixed urinary incontinence, enuresis, nocturnal enuresis, continuous urinary incontinence, and the like), and bladder sensation (increased bladder sensation, reduced bladder sensation, absent bladder sensation, non-specific bladder sensation, and the like). The compound (I) of the present invention or a pharmaceutically acceptable salt thereof is preferably used for treatment or prevention of urinary urgency, increased daytime frequency, nocturia, urge urinary incontinence, mixed urinary incontinence, enuresis, nocturnal enuresis, increased bladder sensation, or non-specific bladder sensation. It is more preferably urinary urgency, increased daytime frequency, nocturia, urge urinary incontinence, or increased bladder sensation. Further, the compound (I) of the present invention or a pharmaceutically acceptable salt thereof is particularly preferably used for treatment or prevention of OABs.
Combinations or Mixtures of Compound (I) of the Present Invention or Pharmaceutically Acceptable Salt Thereof
The compound (I) of the present invention or a pharmaceutically acceptable salt thereof can be appropriately used in combination with at least one agent other than the EP1 receptor antagonist.
Examples of the agent that can be used in combination with the compound (I) of the present invention or a pharmaceutically acceptable salt thereof include agents for the treatment of overactive bladder (OAB), benign prostatic hyperplasia (BPH), cystitis such as interstitial cystitis and the like, prostatitis, and the like, which have different action mechanisms from that of the EP1 receptor antagonist. Examples of the agent include an anticholinergic agent, an α1 antagonist, a β agonist, a 5α-reductase inhibitor, a PDE inhibitor, an acetylcholine esterase inhibitor, an anti-androgen, a progesterone-based hormone, an LH-RH analog, a neurokinin inhibitor, an anti-diuretic, a calcium channel blocker, a direct smooth muscle agonist, a tricyclic antidepressant, a K channel modulator, a sodium channel blocker, an H1 blocker, a serotonin reuptake inhibitor, a norepinephrine reuptake inhibitor, a dopamine reuptake inhibitor, a GABA agonist, a TRPV1 modulator, an endothelin antagonist, a 5-HT1A antagonist, an α1 agonist, an opioid agonist, a P2X antagonist, a COX inhibitor, a σ agonist, a muscarinic agonist, and the like. It is preferably an anticholinergic agent, an α1 antagonist, a β agonist, a 5α-reductase inhibitor, a PDE inhibitor, a progesterone-based hormone, an anti-diuretic, a direct smooth muscle agonist, or a tricyclic antidepressant.
Furthermore, concrete examples of the agent that is used in combination are illustrated as below, but the context of the present invention is not limited thereto. Further, examples of the concrete compound include a free form thereof, and other pharmaceutically acceptable salts.
Examples of the “anticholinergic agent” include oxybutynin, propiverine, solifenacin, tolterodine, imidafenacin, temiberin, darifenacin, fesoterodine, trospium, propantheline, and the like.
Examples of the “α1 antagonist” include urapidil, naphthopidil, tamsulosin, silodosin, prazosin, terazosin, alfuzosin, doxazosin, CR-2991, fiduxosin, and the like.
Examples of the “β agonist” include YM-178, KUC-7483, KRP-204, SM-350300, TRK-380, amibegron, clenbuterol, SAR-150640, solabegron, and the like.
Examples of the “5α-reductase inhibitor” include dutasteride, TF-505, finasteride, izonsteride, and the like.
Examples of the “PDE inhibitor” include tadalafil, vardenafil, sildenafil, avanafil, UK-369003, T-0156, AKP-002, etazolate, and the like.
Examples of the “acetylcholine esterase inhibitor” include distigmine, donepezil, Z-338, rivastigmine, ganstigmine, BGC-20-1259, galantamine, itopride, NP-61, SPH-1286, tolserine, ZT-1, and the like.
Examples of the “anti-androgen” include gestonorone, oxendolone, bicalutamide, BMS-641988, CB-03-01, CH-4892789, flutamide, MDV-3100, nilutamide, TAK-700, YM-580, and the like.
Examples of the “progesterone-based hormone” include chlormadinone, allylestrenol, and the like.
Examples of the “LH-RH analog” include AEZS-108, buserelin, deslorelin, goserelin, histrelin, leuprorelin, lutropin, nafarelin, triptorelin, AEZS-019, cetrorelix, degarelix, elagolix, ganirelix, ozarelix, PTD-634, TAK-385, teverelix, TAK-448, TAK-683, and the like.
Examples of the “neurokinin inhibitor” include KRP-103, aprepitant, AV-608, casopitant, CP-122721, DNK-333, fosaprepitant, LY-686017, netupitant, orvepitant, rolapitant, TA-5538, T-2328, vestipitant, AZD-2624, Z-501, 1144814, MEN-15596, MEN-11420, SAR-102779, SAR-102279, saredutant, SSR-241586, and the like.
Examples of the “anti-diuretic” include desmopressin, VA-106483, and the like.
Examples of the “calcium channel blocker” include amlodipine, cilnidipine, propiverine, temiverine, PD-299685, aranidipine, azelnidipine, barnidipine, benidipine, bevantolol, clevidipine, CYC-381, diltiazem, efonidipine, fasudil, felodipine, gabapentin, gallopamil, isradipine, lacidipine, lercanidipine, lomerizine, manidipine, MEM-1003, nicardipine, nifedipine, nilvadipine, nimodipine, nisoldipine, SB-751689, verapamil, YM-58483, ziconotide, and the like.
Examples of the “direct smooth muscle agonist” include flavoxate and the like.
Examples of the “tricyclic antidepressant” include imipramine, clomipramine, amitriptyline, and the like.
Examples of the “K channel modulator” include nicorandil, NIP-141, NS-4591, NS-1643, andolrast, diazoxide, ICA-105665, minoxidil, pinacidil, tilisolol, VRX-698, and the like.
Examples of the “sodium channel blocker” include bepridil, dronedarone, propafenone, safinamide, SUN-N8075, SMP-986, 1014802, 552-02, A-803467, brivaracetam, cibenzoline, eslicarbazepine, F-15845, flecamide, fosphenyloin, lacosamide, lamotrigine, levobupivacaine, M-58373, mexiletine, moracizine, nerispirdine, NW-3509, oxcarbazepine, pilsicamide, pirmenol, propafenone, NW-1029, ropivacaine, vernakalant, and the like.
Examples of the “H1 blocker” include acrivastine, alcaftadine, bepotastine, bilastine, cetirizine, desloratadine, ebastine, efletirizine, epinastine, fexofenadine, GSK-835726, levocabastine, levocetirizine, loratadine, mequitazine, mizolastine, NBI-75043, ReN-1869, terfenadine, UCB-35440, vapitazine, YM-344484, diphenhydramine, chlorpheniramine, and the like.
Examples of the “serotonin reuptake inhibitor” include UCB-46331, 424887, AD-337, BGC-20-1259, BMS-505130, citalopram, dapoxetine, desvenlafaxine, DOV-102677, DOV-216303, DOV-21947, duloxetine, escitalopram, F-2695, F-98214-TA, fluoxetine, fluvoxamine, IDN-5491, milnacipran, minaprine, NS-2359, NSD-644, paroxetine, PF-184298, SD-726, SEP-225289, SEP-227162, SEP-228425, SEP-228432, sertraline, sibutramine, tesofensine, tramadol, trazodone, UCB-46331, venlafaxine, vilazodone, WAY-426, WF-516, and the like.
Examples of the “norepinephrine reuptake inhibitor” include AD-337, desvenlafaxine, DOV-102677, DOV-216303, DOV-21947, duloxetine, F-2695, F-98214-TA, milnacipran, NS-2359, NSD-644, PF-184298, SD-726, SEP-225289, SEP-227162, SEP-228425, SEP-228432, sibutramine, tesofensine, tramadol, venlafaxine, bupropion, radafaxine, atomoxetine, DDP-225, LY-2216684, neboglamine, NRI-193, reboxetine, tapentadol, WAY-256805, WAY-260022, and the like.
Examples of the “dopamine reuptake inhibitor” include DOV-102677, DOV-216303, DOV-21947, IDN-5491, NS-2359, NSD-644, SEP-225289, SEP-228425, SEP-228432, sibutramine, tesofensine, tramadol, brasofensine, bupropion, NS-27100, radafaxine, safinamide, and the like.
Examples of the “GABA agonist” include retigabine, eszopiclone, indiplon, pagoclone, SEP-225441, acamprosate, baclofen, AZD-7325, BL-1020, brotizolam, DP-VPA, progabide, propofol, topiramate, zopiclone, EVT-201, AZD-3043, ganaxolone, NS-11394, arbaclofen, AZD-3355, GS-39783, ADX-71441, ADX-71943, and the like.
Examples of the “TRPV1 modulator” include capsaicin, resiniferatoxin, DE-096, GRC-6211, AMG-8562, JTS-653, SB-705498, A-425619, A-784168, ABT-102, AMG-628, AZD-1386, JNJ-17203212, NGD-8243, PF-3864086, SAR-115740, SB-782443, and the like.
Examples of the “endothelin antagonist” include SB-234551, ACT-064992, ambrisentan, atrasentan, bosentan, clazosentan, darusentan, fandosentan, S-0139, TA-0201, TBC-3711, zibotentan, BMS-509701, PS-433540, and the like.
Examples of the “5-HT1A antagonist” include espindolol, lecozotan, lurasidone, E-2110, REC-0206, SB-649915, WAY-426, WF-516, and the like.
Examples of the “α1 agonist” include CM-2236, armodafinil, midodrine, moclafinil, and the like.
Examples of the “opioid agonist” include morphine, TRK-130, DPI-125, DPI-3290, fentanyl, LIF-301, loperamide, loperamide oxide, remifentanil, tapentadol, WY-16225, oxycodone, PTI-202, PTI-721, ADL-5747, ADL-5859, DPI-221, DPI-353, IPP-102199, SN-11, ADL-10-0101, ADL-10-0116, asimadoline, buprenorphine, CR-665, CR-845, eptazocine, nalbuphine, nalfurafine, pentazocine, XEN-0548, W-212393, ZP-120, nalmefene, and the like.
Examples of the “P2X antagonist” include A-740003, AZ-11657312, AZD-9056, GSK-1482160, GSK-31481A, and the like.
Examples of the “COX inhibitor” include aceclofenac, ST-679, aspirin, bromfenac, dexketoprofen, flurbiprofen, FYO-750, ibuprofen, ketoprofen, ketorolac, licofelone, lornoxicam, loxoprofen, LT-NS001, diclofenac, mofezolac, nabumetone, naproxen, oxaprozin, piroxicam, pranoprofen, suprofen, tenoxicam, tiaprofenic acid, tolfenamic acid, zaltoprofen, 644784, ABT-963, ajulemic acid, apricoxib, celecoxib, cimicoxib, etoricoxib, iguratimod, lumiracoxib, meloxicam, nimesulide, parecoxib, RO-26-2198, valdecoxib, and the like.
Examples of the “σ agonist” include ANAVEX-27-1041, PRS-013, SA-4503, ANAVEX-2-73, siramesine, ANAVEX-7-1037, ANAVEX-1-41, and the like.
Examples of the “muscarinic agonist” include AC-260584, cevimeline, MCD-386, NGX-267, NGX-292, sabcomeline, pilocarpine, bethanechol, and the like.
When the compound (I) of the present invention or a pharmaceutically acceptable salt thereof is used in combination with one or more of the above-described agents, the present invention includes at least one administration method selected from 1) to 5) below:
1) simultaneous administration by a combination preparation,
2) simultaneous administration by the same administration pathway as a separate formulation,
3) simultaneous administration by a different administration pathway as a separate formulation,
4) administration at different times by the same administration pathway as a separate formulation, or
5) administration at different times by a different administration pathway as a separate formulation.
Further, in the case of administration at different times as a separate formulation as in 4) or 5), the order of administration of the compound (I) of the present invention and the above-described agents is not particularly limited.
Furthermore, the compound (I) of the present invention or a pharmaceutically acceptable salt thereof can be used appropriately in combination of one or more of the above-described agents to attain an advantageous effect that is equal to or more than an additive effect in prevention or treatment of the above-described diseases. Alternatively, as compared with a case of being used alone, the amount used can be reduced, the side effects of the agent used together can be reduced, or the side effects of the agent used together can be avoided or mitigated.
Usage/Dose of Compound (I) of the Present Invention
The pharmaceutical of the present invention can be administered systematically or locally, orally or parenterally (nasal, pulmonary, intravenous, rectal, subcutaneous, intramuscular, transdermal routes, and the like).
When the pharmaceutical composition of the present invention is used for practical treatments, the dose of the compound (I) of the present invention or a pharmaceutically acceptable salt thereof that is the active ingredient is appropriately determined by taking the patient's age, gender, weight, medical condition, degree of the treatment, and the like into consideration. For example, in case of oral administration, administration can be conducted appropriately at a daily dose in the range from about 0.01 to 1000 mg for an adult (as a body weight of 60 kg), and in case of parenteral administration, administration can be conducted appropriately at a daily dose in the range from about 0.001 to 300 mg for an adult in one portion or in several divided portions. In addition, the dose of the compound (I) of the present invention or a pharmaceutically acceptable salt thereof can be reduced according to the amount of the agent other than an EP1 receptor antagonist.
Hereinbelow, the present invention is illustrated in detail with reference to Examples, Reference Examples, and Test Examples, but the scope of the present invention is not limited thereto.
In the symbols used in each of Reference Examples, Examples, and Tables, Ref. No. means Reference Example No., Ex. No. means Example No., Strc means a chemical structural formula, Physical data means physical property values, 1H-NMR means a proton nuclear magnetic resonance spectrum, CDCl3 means chloroform-d, and DMSO-d6 means dimethylsulfoxide-d6. Further, MS means mass spectroscopy, and ESI means measurement by an electrospray ionization method.
To a mixture of 2-bromo-4-trifluoromethoxyaniline (0.500 g), cupper iodide (I) (18.6 mg), bis(triphenylphosphine)palladium (II) dichloride (68.5 mg), triethylamine (0.817 mL) and tetrahydrofuran (7.8 mL) was added phenylacetylene (0.279 mL) at room temperature under stirring, and the mixture was stirred at 80° C. for 16 hours. The reaction mixture was cooled to room temperature, diluted with diethyl ether (30 mL) and then filtered through celite (registered trademark). The filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (206 mg).
1H-NMR (CDCl3) δ ppm:
4.32 (2H, br s), 6.70 (1H, d, J=8.8 Hz), 6.95-7.10 (1H, m), 7.20-7.30 (1H, m), 7.30-7.45 (3H, m), 7.45-7.60 (2H, m).
To a solution of potassium tert-butoxide (173 mg) in 1-methyl-2-pyrrolidone (3.7 mL) was added dropwise a solution of 2-phenylethynyl-4-trifluoromethoxyaniline (204 mg) in 1-methyl-2-pyrrolidone (3.7 mL) at room temperature under stirring, and the mixture was stirred for 6 hours. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic layer was washed with water, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (171 mg).
1H-NMR (CDCl3) δ ppm:
6.80-6.90 (1H, m), 7.00-7.15 (1H, m), 7.30-7.55 (5H, m), 7.60-7.75 (2H, m), 8.42 (1H, br s).
To a solution of tert-butyl (4-chloro-2-methylphenyl)carbamate (338 mg) in tetrahydrofuran (6 mL) was added dropwise sec-butyllithium (1.04 mol/L hexane-cyclohexane solution, 2.69 mL) at −70° C. under an argon atmosphere. The mixture was warmed to −40° C. and then stirred for 10 minutes. Then a solution of N-methoxy-N,2-dimethylbutanamide (203 mg) in tetrahydrofuran (0.5 mL) was added dropwise, and the mixture was stirred at −40° C. for 40 minutes. The mixture was stirred at room temperature for additional 2 hours. 1 mol/L Hydrochloric acid (2.8 mL) was added to the reaction mixture under cooling with ice, and this resulting mixture was extracted with diethyl ether. The organic layer was washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue (446 mg) was dissolved in dichloromethane (4 mL). Trifluoroacetic acid (0.8 mL) was added and this resulting mixture was stirred at room temperature for 14 hours. The reaction mixture was diluted with dichloromethane, washed successively with water and a saturated aqueous sodium hydrogen carbonate solution, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (173 mg).
1H-NMR (CDCl3) δ ppm:
0.91 (3H, t, J=7.4 Hz), 1.33 (3H, d, J=7.0 Hz), 1.55-1.80 (2H, m), 2.75-2.90 (1H, m), 6.15-6.25 (1H, m), 7.06 (1H, dd, J=2.0, 8.5 Hz), 7.21 (1H, d, J=8.5 Hz), 7.45-7.55 (1H, m), 7.90 (1H, br s).
To a solution of 2-fluoro-5-methyl-4-nitroanisole (100 mg) in tetrahydrofuran-ethanol (1/1, 3 mL) was added 10% palladium on carbon powder (56.5 wt % aqueous, 30 mg) under cooling with ice, and the mixture was stirred at room temperature for 5 hours under a hydrogen atmosphere. The reaction mixture was filtered through celite (registered trademark). The filtrate was concentrated under reduced pressure to obtain the title compound (84.8 mg).
1H-NMR (CDCl3) δ ppm:
2.12 (3H, s), 3.42 (2H, br s), 3.81 (3H, s), 6.46 (1H, d, J=12.5 Hz), 6.70 (1H, d, J=9.3 Hz).
To a solution of 5-fluoro-4-methoxy-2-methylaniline (900 mg) in tetrahydrofuran (12 mL) was added di-tert-butyl dicarbonate (1.27 g) at room temperature, and the mixture was stirred overnight at 60° C. The reaction mixture was concentrated under reduced pressure. The residue was purified by aminopropylated silica gel column chromatography (eluting solvent: ethyl acetate-hexane). The fractions of the target were combined and the solvents were evaporated under reduced pressure. Hexane was added to the residue. The precipitate was collected by filtration, washed with hexane and dried under reduced pressure to obtain the title compound (1.24 g).
1H-NMR (CDCl3) δ ppm:
1.51 (9H, s), 2.20 (3H, s), 3.85 (3H, s), 6.09 (1H, br s), 6.74 (1H, d, J=9.0 Hz), 7.30-7.80 (1H, m).
To a solution of tert-butyl (4-chloro-2-methyphenyl)carbamate (483 mg) in tetrahydrofuran (7 mL) was added dropwise sec-butyllithium (1.04 mol/L hexane-cyclohexane solution, 4.3 mL) at −40° C. under an argon atmosphere, and the mixture was stirred for 15 minutes. Then a solution of N-methoxy-N,3-methylbutylamide (319 mg) in tetrahydrofuran (1 mL) was added dropwise, and the mixture was stirred at −40° C. for 15 minutes and at room temperature for 2 hours. Water and 1 mol/L hydrochloric acid were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The aqueous layer was extracted once again with ethyl acetate. The combined organic layers were washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (282 mg).
In addition, structural formula and spectral data of the title compound were shown in Table 1.
The compounds shown in Tables 1 to 2 were synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm: 0.91 (6H, d, J = 6.5 Hz), 1.51 (9H, s), 2.05-2.25 (1H, m), 2.45 (2H, d, J = 6.8 Hz), 3.64 (2H, s), 7.12 (1H, d, J = 2.5 Hz), 7.23 (1H, dd, J = 2.5, 8.8 Hz), 7.35-7.90 (2H, m).
1H-NMR (CDCl3) δ ppm: 0.86 (3H, t, J = 7.5 Hz), 1.11 (3H, d, J = 6.8 Hz), 1.35-1.55 (10H, m), 1.65-1.80 (1H, m), 2.55-2.70 (1H, m), 3.71 (2H, s), 3.77 (3H, s), 6.67 (1H, d, J = 3.0 Hz), 6.80 (1H, dd, J = 3.0, 8.8 Hz), 7.11 (1H, br s), 7.40-7.65 (1H, m).
1H-NMR (CDCl3) δ ppm: 0.83 (6H, t, J = 7.5 Hz), 1.40-1.80 (13H, m), 2.45-2.60 (1H, m), 3.70 (2H, s), 3.77 (3H, s), 6.66 (1H, d, J = 2.9 Hz), 6.81 (1H, dd, J = 2.9, 8.8 Hz), 7.11 (1H, br s), 7.40-7.65 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.49 (9H, s), 3.76 (3H, s), 4.15 (2H, s), 6.74 (1H, d, J = 2.9 Hz), 6.81 (1H, dd, J = 2.9, 8.8 Hz), 7.20-7.70 (4H, m), 8.15-8.25 (1H, m).
1H-NMR (CDCl3) δ ppm: 0.82 (6H, t, J = 7.4 Hz), 1.40-1.75 (13H, m), 2.45-2.60 (1H, m), 3.68 (2H, s), 7.10 (1H, d, J = 2.5 Hz), 7.23 (1H, dd, J = 2.5, 8.8 Hz), 7.40-7.85 (2H, m).
1H-NMR (CDCl3) δ ppm: 1.50 (9H, s), 3.76 (3H, s), 4.00 (2H, s), 6.71 (1H, d, J = 2.8 Hz), 6.75-6.85 (2H, m), 7.15-7.70 (3H, m), 8.16 (1H, s).
1H-NMR (CDCl3) δ ppm: 0.85-1.00 (2H, m), 1.05-1.15 (2H, m), 1.50 (9H, s), 1.95-2.10 (1H, m), 3.78 (3H, s), 3.80 (2H, s), 6.74 (1H, d, J = 2.9 Hz), 6.81 (1H, dd, J = 2.9, 8.8 Hz), 7.00 (1H, br s), 7.40-7.70 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.49 (9H, s), 3.83 (3H, s), 4.24 (2H, s), 6.76 (1H, d, J = 9.0 Hz), 7.40-7.70 (5H, m), 8.00-8.15 (2H, m).
To a solution of tert-butyl[4-chloro-2-(4-methyl-2-oxopentyl)phenyl]carbamate (280 mg) in dichloromethane (4 mL) was added trifluoroacetic acid (0.7 mL) at room temperature, and the mixture was stirred for 7 hours. The reaction mixture was diluted with ethyl acetate and stopped the reaction by addition of a saturated aqueous sodium hydrogen carbonate solution. The organic layer was washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (166 mg).
In addition, structural formula and spectral data of the title compound were shown in Table 3.
The compounds shown in Tables 3 to 4 were synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm: 0.97 (6H, d, J = 6.8 Hz), 1.90-2.05 (1H, m), 2.61 (2H, d, J = 7.3 Hz), 6.15-6.25 (1H, m), 7.06 (1H, dd, J = 2.0, 8.5 Hz), 7.20 (1H, d, J = 8.5 Hz), 7.45-7.55 (1H, m), 7.87 (1H, br s).
1H-NMR (CDCl3) δ ppm: 0.91 (3H, t, J = 7.4 Hz), 1.32 (3H, d, J = 7.0 Hz), 1.55-1.80 (2H, m), 2.75-2.90 (1H, m), 3.84 (3H, s), 6.15-6.20 (1H, m), 6.77 (1H, dd, J = 2.4, 8.8 Hz), 7.02 (1H, d, J = 2.4 Hz), 7.19 (1H, d, J = 8.8 Hz), 7.77 (1H, br s).
1H-NMR (CDCl3) δ ppm: 0.86 (6H, t, J = 7.4 Hz), 1.50-1.85 (4H, m), 2.45-2.65 (1H, m), 3.84 (3H, s), 6.15-6.25 (1H, m), 6.77 (1H, dd, J = 2.3, 8.7 Hz), 7.02 (1H, d, J = 2.3 Hz), 7.20 (1H, d, J = 8.7 Hz), 7.74 (1H, br s).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 6.60-6.70 (1H, m), 6.85 (1H, dd, J = 2.4, 8.8 Hz), 7.07 (1H, d, J = 2.4 Hz), 7.00-7.10 (1H, m), 7.20-7.35 (1H, m), 7.35-7.45 (3H, m), 8.11 (1H, br s).
1H-NMR (CDCl3) δ ppm: 0.86 (6H, t, J =7.4 Hz), 1.50-1.85 (4H, m), 2.45-2.65 (1H, m), 6.15-6.25 (1H, m), 7.06 (1H, dd, J = 2.0, 8.6 Hz), 7.21 (1H, d, J = 8.6 Hz), 7.49 (1H, d, J = 2.0 Hz), 7.86 (1H, br s).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 6.50-6.60 (1H, m), 6.65-6.75 (1H, m), 6.83 (1H, dd, J = 2.4, 8.7 Hz), 7.05 (1H, d, J = 2.4 Hz), 7.20-7.30 (1H, m), 7.45-7.55 (1H, m), 7.70-7.80 (1H, m), 7.98 (1H, br s).
1H-NMR (CDCl3) δ ppm: 0.70-0.80 (2H, m), 0.90-1.00 (2H, m), 1.90-2.00 (1H, m), 3.83 (3H, s), 6.05-6.15 (1H, m), 6.76 (1H, dd, J = 2.4, 8.7 Hz), 6.98 (1H, d, J = 2.4 Hz), 7.16 (1H, d, J = 8.7 Hz), 7.81 (1H, br s).
1H-NMR (CDCl3) δ ppm: 3.94 (3H, s), 6.70-6.80 (1H, m), 7.10-7.20 (2H, m), 7.25-7.50 (3H, m), 7.55-7.70 (2H, m), 8.23 (1H, br s).
To a suspension of 5-hydroxy-2-nitrobenzaldehyde (500 mg) and cesium carbonate (1.46 g) in N,N-dimethylformamide (10 mL) was added ethyl iodide (0.265 mL) at room temperature, and the mixture was stirred for 2 days. Water was added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with water and saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (519 mg).
1H-NMR (CDCl3) δ ppm:
1.48 (3H, t, J=7.0 Hz), 4.18 (2H, q, J=7.0 Hz), 7.13 (1H, dd, J=2.9, 9.1 Hz), 7.31 (1H, d, J=2.9 Hz), 8.16 (1H, d, J=9.1 Hz), 10.49 (1H, s).
To a solution of 5-ethoxy-2-nitrobenzaldehyde (519 mg) and triphenylphosphine (2.09 g) in dichloromethane (13 mL) was added a solution of carbon tetrabromide (1.32 g) in dichloromethane in three minutes under cooling with ice, and the mixture was stirred overnight at room temperature. Hexane (20 mL) was added to the reaction mixture, and this resulting mixture was stirred for 10 minutes and then filtered through silica gel. The filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (712 mg).
1H-NMR (CDCl3) δ ppm:
1.47 (3H, t, J=6.9 Hz), 4.15 (2H, q, J=6.9 Hz), 6.90-7.05 (2H, m), 7.80 (1H, s), 8.17 (1H, d, J=9.1 Hz).
To a suspension of 2-(2,2-dibromovinyl)-4-ethoxynitrobenzene (200 mg) in methanol (3 mL) was added 1% platinum on activated carbon (vanadium doped, 50% hydrous, 27.5 mg) at room temperature, and the mixture was stirred for six and a half hours under a hydrogen atmosphere. 1% Platinum on activated carbon (vanadium doped, 50% hydrous, 27.5 mg) was added, and the mixture was stirred for additional one hour under a hydrogen atmosphere. The reaction mixture was filtered through celite (registered trademark) and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (155 mg).
1H-NMR (CDCl3) δ ppm:
1.38 (3H, t, J=7.0 Hz), 3.44 (2H, br s), 3.97 (2H, q, J=7.0 Hz), 6.55-7.00 (3H, m), 7.34 (1H, s).
A suspension of 2-(2,2-dibromovinyl)-4-ethoxyaniline (240 mg), methyl 3-(bromomethyl)benzoate (180 mg) and potassium carbonate (124 mg) in N,N-dimethylformamide (2 mL) was stirred overnight at room temperature. Water was added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (258 mg).
1H-NMR (CDCl3) δ ppm:
1.37 (3H, t, J=6.9 Hz), 3.70-3.85 (1H, m), 3.85-4.05 (5H, m), 4.38 (2H, d, J=5.5 Hz), 6.52 (1H, d, J=8.8 Hz), 6.70-6.85 (1H, m), 6.90-7.00 (1H, m), 7.34 (1H, s), 7.35-7.50 (1H, m), 7.50-7.65 (1H, m), 7.90-8.15 (2H, m).
To a solution of 5-methoxy-2-phenylindole (245 mg) in N,N-dimethylformamide (4.5 mL) was added sodium hydride (dispersed in liquid paraffin, minimum 55%, 72 mg) under cooling with ice, and the mixture was stirred at room temperature for 75 minutes. Then a solution of 3-(benzyloxy)benzyl bromide (365 mg) in N,N-dimethylformamide (1 mL) was added dropwise, and the mixture was stirred at 80° C. for 15 hours. The reaction mixture was cooled to room temperature. A saturated aqueous ammonium chloride solution-water (2/1) were added and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by aminopropylated silica gel column chromatography to obtain the title compound (202 mg).
1H-NMR (CDCl3) δ ppm:
3.87 (3H, s), 4.94 (2H, s), 5.29 (2H, s), 6.55-6.60 (1H, m), 6.60-6.70 (2H, m), 6.75-6.90 (2H, m), 7.06 (1H, d, J=8.8 Hz), 7.13 (1H, d, J=2.3 Hz), 7.19 (111, t, J=8.0 Hz), 7.25-7.50 (10H, m).
1-(3-Benzyloxybenzyl)-5-methoxy-2-phenylindole (200 mg) was dissolved in trifluoroacetic acid-water-dimethyl sulfide (95/5/10, 4.8 mL), and the solution was stirred at room temperature for 68 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in ethyl acetate. The solution was washed successively with a saturated aqueous sodium hydrogen carbonate solution and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (110 mg).
1H-NMR (CDCl3) δ ppm:
3.87 (3H, s), 4.71 (1H, s), 5.28 (2H, s), 6.40-6.50 (1H, m), 6.55-6.60 (1H, m), 6.60-6.75 (2H, m), 6.81 (1H, dd, J=2.5, 8.9 Hz), 7.07 (1H, d, J=8.9 Hz), 7.10-7.20 (2H, m), 7.30-7.50 (5H, m).
To a solution of tert-butyl (4-methoxy-2-methylphenyl)carbamate (475 mg) in tetrahydrofuran (7 mL) was added dropwise sec-butyllithium (1.04 mol/L hexane-cyclohexane solution, 4.3 mL) at −40° C. under an argon atmosphere, and the mixture was stirred for 15 minutes. Then a solution of trimethylacetaldehyde (0.287 mL) in tetrahydrofuran (1 mL) was added dropwise, and the mixture was stirred at −40° C. for 15 minutes and at room temperature for additional one hour. Water and μmol/L hydrochloric acid were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with water and saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (234 mg).
1H-NMR (CDCl3) δ ppm:
1.00 (9H, s), 1.50 (9H, s), 1.90-2.20 (1H, br), 2.55-2.75 (2H, m), 3.35-3.50 (1H, m), 3.78 (3H, s), 6.65-6.80 (2H, m), 7.20-7.60 (2H, m).
To a solution of tert-butyl[4-methoxy-2-(2-hydroxy-3,3-dimethylbutyl)phenyl]carbamate (234 mg) in dichloromethane (2 mL) was added trifluoroacetic acid (1 mL) at room temperature, and the mixture was stirred for one hour. The reaction mixture was poured into 5% aqueous sodium hydrogen carbonate solution (40 mL) and extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure to obtain the title compound (157 mg).
1H-NMR (CDCl3) δ ppm:
1.01 (9H, s), 2.40-3.70 (5H, m), 3.75 (3H, s), 6.60-6.70 (3H, m).
To a solution of 1-(2-amino-5-methoxyphenyl)-3,3-dimethylbutan-2-ol (155 mg) and methyl 3-formylbenzoate (137 mg) in acetic acid (2 mL) was added sodium triacetoxyborohydride (294 mg) at room temperature, and the mixture was stirred for one hour. The reaction mixture was diluted with ethyl acetate and washed with 5% aqueous sodium hydrogen carbonate solution. The organic layer was washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (205 mg).
1H-NMR (CDCl3) δ ppm:
1.00 (9H, s), 2.60-2.75 (2H, m), 3.40-3.55 (1H, m), 3.74 (3H, s), 3.91 (3H, s), 4.25-4.40 (2H, m), 6.56 (1H, d, J=8.5 Hz), 6.60-6.75 (2H, m), 7.40 (1H, t, J=7.7 Hz), 7.55-7.65 (1H, m), 7.90-8.00 (1H, m), 8.05-8.15 (1H, m).
To a solution of tert-butyl (4-methoxy-2-methylphenyl)carbamate (475 mg) in tetrahydrofuran (7 mL) was added dropwise sec-butyllithium (1.04 mol/L hexane-cyclohexane solution, 4.3 mL) at −40° C. under an argon atmosphere, and the mixture was stirred for 15 minutes. Then a solution of cyclopentanecarboxyaldehyde (0.256 mL) in tetrahydrofuran (1 mL) was added dropwise, and the mixture was stirred at −40° C. for 15 minutes and at room temperature for additional one hour. Water and 1 mol/L hydrochloric acid were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with water and saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (535 mg).
1H-NMR (CDCl3) δ ppm:
1.20-2.10 (19H, m), 2.70 (1H, dd, J=8.3, 14.0 Hz), 2.78 (1H, dd, J=2.9, 14.0 Hz), 3.55-3.70 (1H, m), 3.78 (3H, s), 6.69 (1H, d, J=3.0 Hz), 6.77 (1H, dd, J=3.0, 8.8 Hz), 7.40-7.70 (2H, m).
To a solution of tert-butyl[2-(2-cyclopentyl-2-hydroxyethyl)-4-methoxyphenyl]carbamate (530 mg) in dichloromethane (3 mL) was added trifluoroacetic acid (1 mL) at room temperature, and the mixture was stirred for one hour. The reaction mixture was poured into 5% aqueous sodium hydrogen carbonate solution (40 mL) and extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. To a solution of the residue and methyl 3-formylbenzoate (318 mg) in acetic acid (3 mL) was added sodium triacetoxyborohydride (684 mg) at room temperature, and the mixture was stirred for one hour. The reaction mixture was diluted with ethyl acetate and washed with 5% aqueous sodium hydrogen carbonate solution. The organic layer was washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (362 mg).
1H-NMR (CDCl3) δ ppm:
1.20-1.70 (6H, m), 1.75-1.90 (2H, m), 1.90-2.10 (1H, m), 2.71 (1H, dd, J=8.3, 14.3 Hz), 2.79 (1H, dd, J=3.0, 14.3 Hz), 3.60-3.80 (4H, m), 3.91 (3H, s), 4.25-4.40 (2H, m), 6.55 (1H, d, J=8.6 Hz), 6.66 (1H, dd, J=2.8, 8.6 Hz), 6.70 (1H, d, J=2.8 Hz), 7.40 (1H, t, J=7.7 Hz), 7.55-7.65 m), 7.90-8.00 (1H, m), 8.00-8.10 (1H, m).
To a solution of 5-hydroxy-2-nitrobenzaldehyde (500 mg) in N,N-dimethylformamide (4.3 mL) were added sodium chlorodifluoroacetate (456 mg), sodium hydroxide (120 mg) and water (0.060 mL) at room temperature, and the mixture was stirred at 125° C. for one hour. The reaction mixture was cooled to room temperature, diluted with water and then extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (240 mg).
1H-NMR (CDCl3) δ ppm:
6.68 (1H, t, J=71.5 Hz), 7.46 (1H, dd, J=2.7, 8.9 Hz), 7.64 (1H, d, J=2.7 Hz), 8.21 (1H, d, J=8.9 Hz), 10.46 (1H, s).
To a solution of 5-difluoromethoxy-2-nitrobenzaldehyde (238 mg) and carbon tetrabromide (545 mg) in dichloromethane (5.4 mL) was added dropwise a solution of triphenylphosphine (863 mg) in dichloromethane (3.6 mL) under cooling with ice. The mixture was gradually warmed to room temperature and stirred for 6 hours. The reaction mixture was concentrated under reduced pressure. The residue was suspended in diethyl ether and this resulting mixture was stirred at room temperature for 16 hours. The precipitate was removed by filtration through celite (registered trademark) and washed with diethyl ether. The filtrate was concentrated under reduced pressure to obtain the title compound (536 mg).
1H-NMR (CDCl3) δ ppm:
6.64 (1H, t, J=71.9 Hz), 7.20-7.30 (1H, m), 7.30-7.35 (1H, m), 7.77 (1H, s), 8.19 (1H, d, J=9.0 Hz).
To a suspension of 2-(2,2-dibromovinyl)-4-difluoromethoxynitrobenzene (534 mg) in ethanol (3.7 mL) was added tin (II) chloride dihydrate (742 mg) at room temperature, and the mixture was heated under reflux for 3 hours. The reaction mixture was cooled to room temperature and concentrated under reduced pressure. 1 mol/L Aqueous sodium hydroxide solution was added to the residue and this resulting mixture was extracted with dichloromethane. The aqueous layer was extracted with dichloromethane. The combined organic layers were washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (168 mg).
1H-NMR (CDCl3) δ ppm:
3.68 (2H, br s), 6.39 (1H, t, J=74.4 Hz), 6.67 (1H, d, J=8.8 Hz), 6.96 (1H, dd, J=2.8, 8.8 Hz), 7.13 (1H, d, J=2.8 Hz), 7.29 (1H, s).
To a mixture of 2-(2,2-dibromovinyl)-4-difluoromethoxyaniline (166 mg), phenylboronic acid (88.5 mg) and potassium phosphate monohydrate (557 mg) was added a mixture of 2-dicyclohexylphosphino-2′,6′-dimethoxybiphenyl (11.9 mg), palladium (II) acetate (3.3 mg) and toluene (2.4 mL) at room temperature, and the mixture was stirred at 100° C. for 3 hours. The reaction mixture was cooled to room temperature, diluted with ethyl acetate and then filtered through celite (registered trademark). The filtrate was washed with saturated saline-water (2/1), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (91.2 mg).
1H-NMR (CDCl3) δ ppm:
6.51 (1H, t, J=75.1 Hz), 6.75-6.85 (1H, m), 7.00 (1H, dd, J=2.4, 8.7 Hz), 7.30-7.40 (3H, m), 7.40-7.50 (2H, m), 7.60-7.70 (2H, m), 8.37 (1H, br s).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.47 (9H, s), 3.76 (3H, s), 4.24 (2H, s), 6.65-7.15 (3H, m), 7.25-7.40 (1H, m), 7.40-7.60 (2H, m), 7.65-7.80 (1H, m), 7.80-7.90 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
3.87 (3H, s), 6.75-6.80 (1H, m), 6.88 (1H, dd, J=2.4, 8.8 Hz), 6.95-7.05 (1H, m), 7.09 (1H, d, J=2.4 Hz), 7.25-7.50 (4H, m), 7.90-8.50 (1H, br).
To a solution of 1-benzenesulfonyl-5-methylsulfanyl-2-phenylindole (264 mg) in tetrahydrofuran-methanol (2/1, 6.9 mL) was added cesium carbonate (680 mg) at room temperature, and the mixture was stirred at 50° C. for 26 hours. The reaction mixture was allowed to cool to ambient temperature and concentrated under reduced pressure. 1 mol/L Hydrochloric acid was added to the residue and this resulting mixture was extracted with ethyl acetate. The organic layer was washed successively with a saturated aqueous sodium hydrogen carbonate solution and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by aminopropylated silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (149 mg).
1H-NMR (CDCl3) δ ppm:
2.53 (3H, s), 6.75-6.80 (1H, m), 7.22 (1H, dd, J=1.9, 8.4 Hz), 7.30-7.50 (4H, m), 7.60-7.70 (3H, m), 8.35 (1H, br s).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.52 (9H, s), 3.76 (3H, s), 4.17 (2H, s), 6.75-6.90 (2H, m), 7.35-8.00 (3H, m), 8.28 (1H, d, J=1.0 Hz).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
3.86 (3H, s), 6.65-6.75 (1H, m), 6.86 (1H, dd, J=2.5, 8.8 Hz), 7.06 (1H, d, J=2.5 Hz), 7.29 (1H, d, J=8.8 Hz), 7.90-8.05 (2H, m), 8.50-9.05 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.47 (9H, s), 3.76 (3H, s), 4.20-4.35 (2H, m), 6.60-7.10 (3H, m), 7.10-7.30 (2H, m), 7.30-7.70 (2H, m), 7.80-7.90 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.87 (3H, s), 6.80-6.95 (2H, m), 7.05-7.35 (5H, m), 7.70-7.85 (1H, m), 8.40-9.15 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.48 (9H, s), 3.75 (3H, s), 4.23 (2H, s), 6.70-7.70 (6H, m), 8.00-8.20 (2H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.86 (3H, s), 6.65-6.75 (1H, m), 6.86 (1H, dd, J=2.5, 8.8 Hz), 7.00-7.20 (3H, m), 7.28 (1H, d, J=8.8 Hz), 7.55-7.65 (2H, m), 7.90-8.40 (1H, br).
To a solution of methyl 5-formylthiophene-3-carboxylate (500 mg) in ethanol (5.9 mL) was added sodium borohydride (55.6 mg) at room temperature in small portions, and the mixture was stirred for one hour. The reaction mixture was concentrated under reduced pressure. A saturated aqueous sodium hydrogen carbonate solution was added to the residue and this resulting mixture was extracted with ethyl acetate. The aqueous layer was extracted once again with ethyl acetate. The combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (403 mg).
1H-NMR (CDCl3) δ ppm:
1.86 (1H, t, J=6.0 Hz), 3.86 (3H, s), 4.75-4.90 (2H, m), 7.35-7.45 (1H, m), 8.04 (1H, d, J=1.3 Hz).
To a solution of methyl 5-hydroxymethylthiophene-3-carboxylate (401 mg) in ethyl acetate (4.7 mL) were added triethylamine (0.390 mL) and methanesulfonyl chloride (0.198 mL) under cooling with ice. The mixture was stirred under cooling with ice for 40 minutes. The reaction mixture was diluted with ethyl acetate (4.7 mL) and filtered through celite (registered trademark). To the filtrate was added lithium bromide monohydrate (733 mg) at room temperature, and the mixture was stirred at 50° C. for 6 hours. The reaction mixture was allowed to cool to ambient temperature, diluted with water and extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (495 mg).
1H-NMR (CDCl3) δ ppm:
3.86 (3H, s), 4.68 (2H, s), 7.45-7.55 (1H, m), 8.07 (1H, d, J=1.3 Hz).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.47 (9H, s), 3.76 (3H, s), 4.24 (2H, s), 6.60-7.20 (3H, m), 7.35-7.65 (3H, m), 7.85-8.10 (2H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.87 (3H, s), 6.75-6.80 (1H, m), 6.88 (1H, dd, J=2.5, 8.8 Hz), 7.05-7.15 (1H, m), 7.20-7.35 (2H, m), 7.36 (1H, t, J=7.8 Hz), 7.45-7.55 (1H, m), 7.60-7.65 (1H, m), 8.00-8.40 (1H, br).
To a solution of 2-bromo-5-chloro-4-methoxyaniline (8.97 g) in pyridine (25.3 mL) was added dropwise trifluoroacetic anhydride (2.81 mL) under cooling with ice. The mixture was stirred at room temperature for 30 hours. Methanol (1.5 mL) was added to the reaction mixture and the stirring was continued for additional 40 minutes. The reaction mixture was concentrated under reduced pressure. 1 mol/L Hydrochloric acid was added to the residue and this resulting mixture was extracted with ethyl acetate. The organic layer was washed successively with 1 mol/L hydrochloric acid, a saturated aqueous sodium hydrogen carbonate solution and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure to obtain the title compound (3.45 g).
1H-NMR (DMSO-d6) δ ppm:
3.92 (3H, s), 7.51 (1H, s), 7.61 (1H, s), 11.21 (1H, s).
To a mixture of N-(2-bromo-5-chloro-4-methoxyphenyl)-2,2,2-trifluoroacetamide (512 mg), phenylacetylene (0.254 mL), copper (I) iodide (17.5 mg), triethylamine (549 mL) and acetonitrile (12.3 mL) was added bis(triphenylphosphine)palladium (II) dichloride (32.5 mg). The mixture was stirred at 120° C. for 2 hours under irradiation of microwave. The reaction mixture was allowed to cool to ambient temperature. Potassium carbonate (532 mg) was added to the reaction mixture. The mixture was stirred at 120° C. for additional 2 hours under irradiation of microwave. The reaction mixture was allowed to cool to ambient temperature and filtered through celite (registered trademark). The filtrate was concentrated under reduced pressure and the residue was purified by aminopropylated silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (255 mg).
1H-NMR (CDCl3) δ ppm:
3.95 (3H, s), 6.70-6.80 (1H, m), 7.13 (1H, s), 7.30-7.50 (4H, m), 7.60-7.70 (2H, m), 8.00-8.40 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 36 by using the corresponding starting material.
1H-NMR (DMSO-d6) δ ppm:
2.11 (3H, s), 3.83 (3H, s), 7.15-7.30 (2H, m), 11.08 (1H, s).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
2.34 (3H, s), 3.88 (3H, s), 6.70-6.80 (1H, m), 7.02 (1H, s), 7.10-7.35 (2H, m), 7.35-7.50 (2H, m), 7.55-7.70 (2H, m), 8.13 (1H, br s).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.95 (3H, s), 6.80-6.95 (1H, m), 7.10-7.35 (4H, m), 7.40-7.50 (1H, m), 7.70-7.85 (1H, m), 8.76 (1H, br s).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.95 (3H, s), 6.70-6.80 (1H, m), 6.95-7.10 (1H, m), 7.12 (1H, s), 7.25-7.50 (4H, m), 8.19 (1H, br s).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.94 (3H, s), 6.60-6.75 (1H, m), 7.10-7.20 (3H, m), 7.35-7.45 (1H, m), 7.55-7.70 (2H, m), 8.13 (1H, br s).
To a solution of methyl 6-ethylpyridine-2-carboxylate (587 mg) in carbon tetrachloride (28.4 mL) were added N-bromosuccinimide (696 mg) and benzoyl peroxide (75%, 11.5 mg). The mixture was heated under reflux for 4 hours. The reaction mixture was allowed to cool to ambient temperature. The insoluble material was removed by filtration and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (702 mg).
1H-NMR (CDCl3) δ ppm:
2.08 (3H, d, J=7.0 Hz), 4.01 (3H, s), 5.34 (1H, q, J=7.0 Hz), 7.70-7.95 (2H, m), 8.00-8.10 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 36 by using the corresponding starting material.
1H-NMR (DMSO-d6) δ ppm:
3.90 (3H, s), 7.47 (1H, d, J=11.8 Hz), 7.54 (1H, d, J=8.8 Hz), 11.21 (1H, s).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.94 (3H, s), 6.80-6.90 (1H, m), 7.05-7.40 (5H, m), 7.65-7.85 (1H, m), 8.55-9.00 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
3.94 (3H, s), 6.70-6.80 (1H, m), 6.95-7.10 (1H, m), 7.10-7.20 (2H, m), 7.25-7.45 (3H, m), 8.00-8.40 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
3.94 (3H, s), 6.60-6.75 (1H, m), 7.05-7.20 (4H, m), 7.50-7.65 (2H, m), 8.00-8.30 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.94 (3H, s), 6.75-6.85 (1H, m), 7.10-7.25 (2H, m), 7.30-7.45 (1H, m), 7.85-8.00 (1H, m), 8.40-8.75 (2H, m), 8.85-9.00 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 5 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
1.51 (9H, s), 2.23 (3H, s), 3.86 (3H, s), 5.65-6.40 (1H, br), 6.72 (1H, s), 7.40-8.10 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.49 (9H, s), 3.85 (3H, s), 4.16 (2H, s), 6.73 (1H, s), 6.90-8.00 (4H, m), 8.15-8.30 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
3.94 (3H, s), 6.55-6.70 (1H, m), 7.10 (1H, s), 7.35-7.50 (4H, m), 7.90-8.35 (1H, br).
To a suspension of 4-benzyloxy-3-chloroaniline (674 mg) and potassium carbonate (1.14 g) in dichloromethane (32 mL) was added dropwise a solution of bromine (1.25 g) in dichloromethane (16 mL) at −15° C. in one hour, and the mixture was stirred at −15° C. for 75 minutes. Water was added to the reaction mixture and this resulting mixture was stirred vigorously for additional 10 minutes. The organic layer was separated, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (1.13 g).
1H-NMR (CDCl3) δ ppm:
3.85 (2H, br s), 5.03 (2H, s), 6.84 (1H, s), 7.07 (1H, s), 7.25-7.55 (5H, m).
The title compound was synthesized in a manner similar to that of Reference Example 36 by using the corresponding starting material.
1H-NMR (DMSO-d6) δ ppm:
5.29 (2H, s), 7.30-7.55 (5H, m), 7.55-7.70 (2H, m), 11.22 (1H, s).
The title compound was synthesized in a manner similar to that of Reference Example 37 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
5.19 (2H, s), 6.65-6.80 (1H, m), 7.18 (1H, s), 7.25-7.75 (11H, m), 8.23 (1H, br s).
To a solution of 5-benzyloxy-6-chloro-2-phenylindole (513 mg) in N,N-dimethylformamide (7.7 mL) was added sodium hydride (in oil, 50 to 72%, 92 mg) under cooling with ice under an argon atmosphere, and the mixture was stirred at room temperature for one hour. Then methyl 6-(chloromethyl)pyridine-2-carboxylate (342 mg) was added, and the mixture was stirred at 80° C. for 18 hours. The reaction mixture was allowed to cool to ambient temperature. A saturated aqueous ammonium chloride solution and water were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (454 mg).
1H-NMR (CDCl3) δ ppm:
4.03 (3H, s), 5.20 (2H, s), 5.54 (2H, s), 6.55-6.65 (1H, m), 6.65-6.75 (1H, m), 7.15-7.60 (12H, m), 7.65-7.75 (1H, m), 7.95-8.10 (1H, m).
A solution of methyl 6-(5-benzyloxy-6-chloro-2-phenylindol-1-ylmethyl)pyridine-2-carboxylate (372 mg) in trifluoroacetic acid/water/dimethyl sulfide (95/5/10, 7.7 mL) was stirred at room temperature for 80 hours. The reaction mixture was concentrated under reduced pressure. A saturated aqueous sodium hydrogen carbonate solution (20 mL) was added to the residue and this resulting mixture was extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was triturated in dichloromethane/hexane (2/1). The precipitate was collected by filtration, washed with dichloromethane/hexane (2/1), and air-dried to obtain the title compound (213 mg).
1H-NMR (CDCl3) δ ppm:
4.03 (3H, s), 5.33 (1H, s), 5.53 (2H, s), 6.57 (1H, s), 6.69 (1H, d, J=7.8 Hz), 7.12 (1H, s), 7.28 (1H, s), 7.30-7.50 (5H, m), 7.65-7.80 (1H, m), 8.01 (1H, d, J=7.5 Hz).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.50 (9H, s), 3.85 (3H, s), 4.01 (2H, s), 6.70 (1H, s), 6.75-6.85 (1H, m), 7.00-8.00 (3H, m), 8.15-8.25 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
3.94 (3H, s), 6.45-6.60 (1H, m), 6.60-6.75 (1H, m), 7.09 (1H, s), 7.35-7.40 (1H, m), 7.45-7.55 (1H, m), 7.70-7.80 (1H, m), 7.80-8.20 (1H, br).
A mixture of 4-amino-2,5-dimethylphenol (2.00 g), di-tert-butyl dicarbonate (3.50 g) and tetrahydrofuran (29 mL) was heated under reflux overnight. The reaction mixture was allowed to cool to ambient temperature and concentrated under reduced pressure to obtain the title compound (3.80 g).
1H-NMR (CDCl3) δ ppm:
1.51 (9H, s), 2.16 (3H, s), 2.18 (3H, s), 4.60-5.00 (1H, br), 5.80-6.25 (1H, br), 6.55 (1H, s), 7.20-7.45 (1H, br).
To a solution of tert-butyl (4-hydroxy-2,5-dimethylphenyl)carbamate (1.25 g) and methyl iodide (1.12 g) in N,N-dimethylformamide (10.5 mL) was added potassium carbonate (1.46 g), and the mixture was stirred at room temperature for 3 hours. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (1.32 g).
1H-NMR (CDCl3) δ ppm:
1.51 (9H, s), 2.17 (3H, s), 2.22 (3H, s), 3.79 (3H, s), 5.75-6.30 (1H, br), 6.62 (1H, s), 7.20-7.55 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.49 (9H, s), 2.18 (3H, s), 3.77 (3H, s), 4.15 (2H, s), 6.61 (1H, s), 6.80-7.70 (4H, m), 8.15-8.30 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
2.30-2.40 (3H, m), 3.88 (3H, s), 6.55-6.65 (1H, m), 7.00 (1H, s), 7.13 (1H, s), 7.30-7.45 (3H, m), 8.02 (1H, br s).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.47 (9H, s), 3.85 (3H, s), 4.29 (2H, s), 6.50-7.20 (2H, m), 7.40-7.90 (2H, m), 8.25-8.35 (1H, m), 8.75-8.90 (1H, m), 9.25-9.35 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
3.95 (3H, s), 6.75-6.85 (1H, m), 7.14 (1H, s), 7.38 (111, dd, J=4.8, 8.0 Hz), 7.45 (1H, s), 7.85-8.00 (1H, m), 8.36 (1H, br s), 8.50-8.65 (1H, m), 8.90-9.00 (1H, m).
To a suspension of sodium hydride (in oil, minimum 55%, 801 mg) in N,N-dimethylformamide (30 mL) was added dropwise a solution of 5-bromo-2-phenylindole (3.33 g) in N,N-dimethylformamide (30 mL) under cooling with ice and under an argon atmosphere, and the mixture was stirred at room temperature for one hour and a half. Then benzenesulfonyl chloride (1.88 mL) was added dropwise and the stirring was continued for additional 17 hours. A saturated aqueous ammonium chloride solution and water were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (1.87 g).
1H-NMR (CDCl3) δ ppm:
6.48 (1H, s), 7.15-7.65 (12H, m), 8.19 (1H, d, J=8.8 Hz).
To a solution of 1-benzenesulfonyl-5-bromo-2-phenylindole (958 mg) in tetrahydrofuran (11.6 mL) was added dropwise n-butyllithium (2.76 mol/L hexane solution, 0.84 mL) at −78° C. under an argon atmosphere, and the mixture was stirred for 30 minutes. Then N,N-dimethylformamide (0.535 mL) was added dropwise, and the mixture was stirred at −78° C. for 30 minutes and at room temperature for 2 hours. A saturated aqueous ammonium chloride solution and water were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (225 mg).
1H-NMR (CDCl3) δ ppm:
6.60-6.70 (1H, m), 7.20-7.55 (10H, m), 7.91 (1H, dd, J=1.6, 8.7 Hz), 7.95-8.05 (1H, m), 8.47 (1H, d, J=8.7 Hz), 10.07 (1H, s).
To a suspension of 1-benzenesulfonyl-5-formyl-2-phenylindole (224 mg) in ethanol (2.5 mL) was added sodium borohydride (11.7 mg), and the mixture was stirred at room temperature for one hour. A saturated aqueous ammonium chloride solution was added to the reaction mixture and the resulting mixture was extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (173 mg).
1H-NMR (CDCl3) δ ppm:
1.68 (1H, br s), 4.77 (2H, s), 6.50-6.60 (1H, m), 7.20-7.55 (12H, m), 8.29 (1H, d, J=8.5 Hz).
To a solution of 1-benzenesulfonyl-5-hydroxymethyl-2-phenylindole (171 mg) in N,N-dimethylformamide (2.4 mL) were added imidazole (128 mg) and chlorotriisopropylsilane (0.150 mL) under cooling with ice, and the mixture was stirred at room temperature for 20 hours. Water was added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with water, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (221 mg).
1H-NMR (CDCl3) δ ppm:
0.95-1.30 (21H, m), 4.90 (2H, s), 6.50-6.60 (1H, m), 7.20-7.55 (12H, m), 8.25 (1H, d, J=8.8 Hz).
The title compound was synthesized in a manner similar to that of Reference Example 25 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
1.00-1.35 (21H, m), 4.93 (2H, s), 6.75-6.85 (1H, m), 7.15-7.25 (1H, m), 7.25-7.40 (2H, m), 7.40-7.50 (2H, m), 7.55-7.75 (3H, m), 8.15-8.45 (1H, br).
The title compound was synthesized in a manner similar to that of Reference Example 55 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
1.05-1.25 (21H, m), 4.03 (3H, s), 4.93 (2H, s), 5.60 (2H, s), 6.65-6.75 (2H, m), 7.08 (1H, d, J=8.3 Hz), 7.10-7.20 (1H, m), 7.30-7.45 (5H, m), 7.60-7.70 (2H, m), 7.95-8.05 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 14 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
0.50-2.30 (19H, m), 2.45-2.95 (2H, m), 3.55-4.05 (4H, m), 6.60-6.80 (1H, m), 7.20-8.00 (2H, m).
The title compound was synthesized in a manner similar to that of Reference Example 15 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
0.75-1.75 (9H, m), 2.45-2.85 (2H, m), 3.30-4.10 (6H, m), 6.60-6.80 (2H, m).
ESI-MS (m/z): 258, 260 (M+H)+.
A mixture of 1-(2-amino-4-chloro-5-methoxyphenyl)-3-methylpentan-2-ol (602 mg), tetrakis(triphenylphosphine)palladium (0) (135 mg), potassium carbonate (646 mg), 2-bromomesitylene (0.420 mL) and N,N-dimethylformamide (11.7 mL) was stirred at 160° C. for one hour under microwave irradiation. The reaction mixture was allowed to cool to ambient temperature. Water was added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (514 mg).
1H-NMR (CDCl3) δ ppm:
0.91 (3H, t, J=7.4 Hz), 1.32 (3H, d, J=6.8 Hz), 1.55-1.80 (2H, m), 2.70-2.90 (1H, m), 3.91 (3H, s), 6.10-6.20 (1H, m), 7.05 (1H, s), 7.25-7.35 (1H, m), 7.50-8.00 (1H, br).
A mixture of tert-butyl (4-iodo-2-methylphenyl)carbamate (1.04 g), cyclopropylboronic acid monohydrate (422 mg), palladium acetate (35.1 mg), tricyclohexylphosphine (87.5 mg), tripotassium phosphate monohydrate (2.52 g), toluene (8.7 mL) and water (0.87 mL) was stirred at 100° C. for 15 hours. The reaction mixture was allowed to cool to ambient temperature, diluted with ethyl acetate and filtered through celite (registered trademark). The filtrate was washed with water/saturated saline (1/1, 20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (668 mg).
1H-NMR (CDCl3) δ ppm:
0.55-0.70 (2H, m), 0.80-1.00 (2H, m), 1.51 (9H, s), 1.75-1.90 (1H, m), 2.21 (3H, s), 5.85-6.45 (1H, br), 6.75-7.00 (2H, m), 7.45-7.75 (1H, m).
The title compound was synthesized in a manner similar to that of Reference Example 6-1 by using the corresponding starting material and reactants.
1H-NMR (CDCl3) δ ppm:
0.55-0.70 (2H, m), 0.80-1.00 (2H, m), 1.49 (9H, s), 1.75-1.90 (1H, m), 4.25 (2H, s), 6.85-7.00 (2H, m), 7.20-7.75 (5H, m), 8.00-8.15 (2H, m).
The title compound was synthesized in a manner similar to that of Reference Example 7-1 by using the corresponding starting material and reactant.
1H-NMR (CDCl3) δ ppm:
0.65-0.75 (2H, m), 0.85-1.00 (2H, m), 1.95-2.10 (1H, m), 6.70-6.80 (1H, m), 6.90-7.00 (1H, m), 7.25-7.50 (5H, m), 7.60-7.70 (2H, m), 8.24 (1H, br s).
To a solution of 5-chloro-2-phenylindole (154 mg) in N,N-dimethylformamide (1.5 mL) was added sodium hydride (dispersed in liquid paraffin, minimum 55%, 31 mg) at room temperature, and the mixture was stirred for 15 minutes. Methyl 6-(chloromethyl)pyridine-2-carboxylate (126 mg) was added and this resulting mixture was stirred at 50 to 60° C. for additional 18 hours. Water, ethyl acetate and a saturated aqueous ammonium chloride solution were added to the reaction mixture. The organic layer was separated. The aqueous layer was extracted with ethyl acetate. The combined organic layers were washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by aminopropylated silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (112 mg).
In addition, structural formula and spectrum data of the title compound are shown in Table 5.
The compounds shown in Tables 5 to 6 were synthesized in a manner similar to that of Example 1-1 by using the corresponding starting materials.
1H-NMR (CDCl3) δ ppm: 4.03 (3H, s), 5.59 (2H, s), 6.60-6.70 (2H, m), 7.00-7.15 (2H, m), 7.35-7.45 (5H, m), 7.60-7.75 (2H, m), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 4.02 (3H, s), 5.59 (2H, s), 6.60-6.75 (2H, m), 6.85-6.95 (1H, m), 7.04 (1H, dd, J = 4.3, 8.8 Hz), 7.25-7.50 (6H, m), 7.60-7.75 (1H, m), 8.00 (1H, d, J = 7.5 Hz).
1H-NMR (CDCl3) δ ppm: 3.87 (3H, s), 4.02 (3H, s), 5.58 (2H, s), 6.60-6.75 (2H, m), 6.81 (1H, dd, J = 2.5, 8.8 Hz), 7.02 (1H, d, J = 8.8 Hz), 7.15 (1H, d, J = 2.5 Hz), 7.30-7.45 (5H, m), 7.60-7.70 (1H, m), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.29 (3H, t, J = 7.5 Hz), 2.75 (2H, q, J = 7.5 Hz), 4.03 (3H, s), 5.59 (2H, s), 6.55-6.80 (2H, m), 6.95-7.10 (2H, m), 7.25-7.60 (6H, m), 7.60-7.75 (1H, m), 7.90-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 4.05 (3H, s), 5.63 (2H, s), 6.60-6.70 (1H, m), 6.70-6.80 (1H, m), 6.81 (1H, dd, J = 2.5, 8.8 Hz), 7.03 (1H, d, J = 8.8 Hz), 7.13 (1H, d, J = 2.5 Hz), 7.16 (1H, dd, J = 1.3, 5.0 Hz), 7.23 (1H, dd, J = 1.3, 3.0 Hz), 7.36 (1H, dd, J = 3.0, 5.0 Hz), 7.68 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.27 (6H, d, J = 6.8 Hz), 2.85-3.00 (1H, m), 3.84 (3H, s), 4.05 (3H, s), 5.55 (2H, s), 6.34 (1H, s), 6.40-6.50 (1H, m), 6.74 (1H, dd, J = 2.5, 8.8 Hz), 6.98 (1H, d, J = 8.8 Hz), 7.08 (1H, d, J = 2.5 Hz), 7.55-7.65 (1H, m), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 4.03 (3H, s), 5.60 (2H, s), 6.30-6.80 (3H, m), 6.95 (1H, dd, J = 2.3, 8.8 Hz), 7.09 (1H, d, J = 8.8 Hz), 7.35-7.50 (6H, m), 7.65-7.75 (1H, m), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 0.93 (6H, d, J = 6.8 Hz), 1.80-1.95 (1H, m), 2.52 (2H, d, J = 7.0 Hz), 3.84 (3H, s), 4.05 (3H, s), 5.51 (2H, s), 6.31 (1H, s), 6.44 (1H, d, J = 7.8 Hz), 6.74 (1H, dd, J = 2.4, 8.9 Hz), 6.99 (1H, d, J = 8.9 Hz), 7.07 (1H, d, J = 2.4 Hz), 7.61 (1H, t, J = 7.8 Hz), 7.99 (1H, d, J = 7.8 Hz).
To a solution of 5-chloro-2-isopropylindole (136 mg) in N,N-dimethylformamide (2 mL) was added sodium hydride (dispersed in liquid paraffin, minimum 55%, 32 mg) under cooling with ice, and the mixture was stirred for 30 minutes. Then ethyl 5-(chloromethyl)furan-2-carboxylate (0.107 mL) was added, and the mixture was stirred at 60° C. for 20 hours. A saturated aqueous ammonium chloride solution was added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (133 mg).
In addition, structural formula and spectrum data of the title compound are shown in Table 7.
The compounds shown in Tables 7 to 8 were synthesized in a manner similar to that of Example 2-1 by using the corresponding starting materials.
1H-NMR (CDCl3) δ ppm: 1.30-1.40 (9H, m), 2.95-3.15 (1H, m), 4.35 (2H, q, J = 7.0 Hz), 5.32 (2H, s), 5.80-5.85 (1H, m), 6.29 (1H, s), 6.95-7.15 (3H, m), 7.45-7.55 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 4.34 (2H, q, J = 7.1 Hz), 5.30 (2H, s), 5.97 (1H, d, J = 3.4 Hz), 6.55 (1H, s), 7.04 (1H, d, J = 3.4 Hz), 7.16 (1H, dd, J = 2.0, 8.7 Hz), 7.22 (1H, d, J = 8.7 Hz), 7.35-7.55 (5H, m), 7.55-7.65 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 4.35 (2H, q, J = 7.1 Hz), 5.30 (2H, s), 5.95-6.05 (1H, m), 6.55-6.60 (1H, m), 6.95 (1H, dt, J = 2.4, 9.0 Hz), 7.05 (1H, d, J = 3.5 Hz), 7.22 (1H, dd, J =4.4, 9.0 Hz), 7.29 (1H, dd, J = 2.4, 9.4Hz), 7.35-7.55 (5H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.2 Hz), 3.87 (3H, s), 4.35 (2H, q, J = 7.2 Hz), 5.29 (2H, s), 5.95-6.05 (1H, m), 6.50-6.60 (1H, m), 6.87 (1H, dd, J = 2.4, 9.0 Hz), 7.05 (1H, d, J = 3.5 Hz), 7.12 (1H, d, J = 2.4 Hz), 7.19 (1H, d, J = 9.0 Hz), 7.35-7.55 (5H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J =7.2 Hz), 3.86 (3H, s), 4.35 (2H, q, J = 7.2 Hz), 5.34 (2H, s), 6.00-6.05 (1H, m), 6.55-6.60 (1H, m), 6.86 (1H, dd, J = 2.5, 8.8 Hz), 7.06 (1H, d, J = 3.3 Hz), 7.09 (1H, d, J = 2 .5 Hz), 7.18 (1H, d, J = 8.8 Hz), 7.24 (1H, dd, J = 1.3, 5.0 Hz), 7.37 (1H, dd, J = 1.3, 2.9 Hz), 7.41 (1H, dd, J = 2.9, 5.0 Hz).
1H-NMR (CDCl3) δ ppm: 1.32 (6H, d, J = 7.0 Hz), 1.36 (3H, t, J = 7.1 Hz), 2.95-3.10 (1H, m), 3.83 (3H, s), 4.35 (2H, q, J = 7.1 Hz), 5.30 (2H, s), 5.80- 5.90 (1H, m), 6.27 (1H, s), 6.78 (1H, dd, J = 2.5, 8.8 Hz), 7.00 (1H, d, J = 3.3 Hz), 7.04 (1H, d, J = 2.5 Hz), 7.10 (1H, d, J = 8.8 Hz).
1H-NMR (CDCl3) δ ppm: 0.98 (6H, d, J = 6.5 Hz), 1.37 (3H, t, J = 7.1 Hz), 1.85-2.05 (1H, m), 2.59 (2H, d, J = 7.3 Hz), 3.84 (3H, s), 4.35 (2H, q, J = 7.1 Hz), 5.27 (2H, s), 5.82 (1H, d, J = 3.5 Hz), 6.24 (1H, s), 6.78 (1H, dd, J = 2.3, 9.0 Hz), 7.00 (1H, d, J = 3.5 Hz), 7.03 (1H, d, J = 2.3 Hz), 7.10 (1H, d, J = 9.0 Hz).
To a solution of 5-methoxy-2-phenylindole (73.0 mg) in N,N-dimethylformamide (1.6 mL) was added sodium hydride (dispersed in liquid paraffin, minimum 55%, 22 mg) under cooling with ice, and the mixture was stirred at room temperature for 70 minutes. Then methyl 3-(bromomethyl)benzoate (89.9 mg) was added, and the mixture was stirred at 100° C. for 6 hours. The reaction mixture was cooled to room temperature. Saturated ammonium chloride-water (2/1) were added and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by aminopropylated silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (50.6 mg).
In addition, structural formula and spectrum data of the title compound are shown in Table 9.
The compounds shown in Tables 9 to 12 were synthesized in a manner similar to that of Example 3-1 by using the corresponding starting materials.
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 3.89 (3H, s), 5.36 (2H, s), 6.55-6.65 (1H, m), 6.80 (1H, dd, J = 2.5, 8.8 Hz), 7.03 (1H, d, J = 8.8 Hz), 7.05-7.20 (2H, m), 7.25- 7.50 (6H, m), 7.75-7.85 (1H, m), 7.85-7.95 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.89 (3H, s), 5.38 (2H, s), 6.55-6.65 (1H, m), 7.00- 7.15 (3H, m), 7.30-7.45 (6H, m), 7.60-7.65 (1H, m), 7.75-7.80 (1H, m), 7.85-7.95 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.29 (6H, d, J = 7.0 Hz), 2.85-3.05 (1H, m), 3.90 (3H, s), 5.37 (2H, s), 6.33 (1H, s), 6.80-7.10 (3H, m), 7.25-7.35 (1H, m), 7.50-7.60 (1H, m), 7.75- 7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.89 (3H, s), 5.39 (2H, s), 6.60-6.70 (1H, m), 6.95- 7.15 (3H, m), 7.30-7.45 (6H, m), 7.50-7.55 (1H, m), 7.75-7.85 (1H, m), 7.90-8.00 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.89 (3H, s), 5.43 (2H, s), 6.74 (1H, s), 7.05-7.15 (1H, m), 7.22 (1H, d, J = 8.8 Hz), 7.30-7.50 (7H, m), 7.75-7.85 (1H, m), 7.90-8.00 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.89 (3H, s), 5.38 (2H, s), 6.62 (1H, s), 6.89 (1H, dt, J = 2.5, 9.0 Hz), 7.00-7.15 (2H, m), 7.25-7.50 (7H, m), 7.75-7.85 (1H, m), 7.85-7.95 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.98 (3H, s), 5.42 (2H, s), 6.70-6.75 (1H, m), 7.00-7.10 (1H, m), 7.20 (1H, d, J = 8.5 Hz), 7.30-7.50 (7H, m), 7.70- 7.80 (1H, m), 7.90-8.05 (2H, m).
1H-NMR (CDCl3) δ ppm: 0.86 (3H, t, J = 7.4 Hz), 1.25 (3H, d, J = 7.0 Hz), 1.45-1.80 (2H, m), 2.65-2.80 (1H, m), 3.89 (3H, s), 5.36 (2H, s), 6.30 (1H, s), 6.85-7.10 (3H, m), 7.20-7.35 (1H, m), 7.50-7.60 (1H, m), 7.75- 7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 0.95 (6H, d, J = 6.5 Hz), 1.80- 2.00 (1H, m), 2.54 (2H, d, J = 7.3 Hz), 3.89 (3H, s), 5.32 (2H, s), 6.30 (1H, s), 6.85- 7.10 (3H, m), 7.20-7.40 (1H, m), 7.45-7.60 (1H, m), 7.75- 7.85 (1H, m), 7.85-8.00 (1H, m).
1H-NMR (CDCl3) δ ppm: 2.45 (3H, s), 3.88 (3H, s), 5.37 (2H, s), 6.55-6.60 (1H, m), 6.90-7.15 (3H, m), 7.25- 7.50 (7H, m), 7.80-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 1.28 (6H, d, J = 6.8 Hz), 2.85-3.05 (1H, m), 3.83 (3H, s), 3.90 (3H, s), 5.35 (2H, s), 6.25-6.35 (1H, m), 6.73 (1H, dd, J = 2.3, 8.9 Hz), 6.85-6.95 (1H, m), 6.99 (1H, d, J = 8.9 Hz), 7.07 (1H, d, J = 2.3 Hz), 7.20-7.35 (1H, m), 7.80-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 0.86 (3H, t, J = 7.4 Hz), 1.24 (3H, d, J = 6.8 Hz), 1.45-1.85 (2H, m), 2.65-2.80 (1H, m), 3.84 (3H, s), 3.89 (3H, s), 5.34 (2H, s), 6.29 (1H, s), 6.74 (1H, dd, J = 2.5, 8.8 Hz), 6.85-7.00 (1H, m), 7.02 (1H, d, J = 8.8 Hz), 7.07 (1H, d, J = 2.5 Hz), 7.29 (1H, t, J = 7.8 Hz), 7.75-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 0.95 (6H, d, J = 6.5 Hz), 1.80- 2.00 (1H, m), 2.54 (2H, d, J = 7.3 Hz), 3.84 (3H, s), 3.89 (3H, s), 5.31 (2H, s), 6.28 (1H, s), 6.73 (1H, dd, J = 2.5, 8.8 Hz), 6.90-7.10 (3H, m), 7.29 (1H, t, J = 7.8 Hz), 7.80-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 0.79 (6H, t, J = 7.4 Hz), 1.55- 1.70 (4H, m), 2.50-2.65 (1H, m), 3.85 (3H, s), 3.89 (3H, s), 5.34 (2H, s), 6.27 (1H, s), 6.74 (1H, dd, J = 2.5, 8.8 Hz), 6.90-7.10 (3H, m), 7.20-7.35 (1H, m), 7.80-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 3.89 (3H, s), 5.42 (2H, s), 6.60-6.65 (1H, m), 6.81 (1H, dd, J = 2.5, 9.0 Hz), 7.04 (1H, d, J = 9.0 Hz), 7.05-7.25 (4H, m), 7.30- 7.40 (2H, m), 7.80-8.00 (2H, m).
1H-NMR (CDCl3) δ ppm: 0.79 (6H, t, J = 7.4 Hz), 1.55- 1.75 (4H, m), 2.50-2.65 (1H, m), 3.89 (3H, s), 5.36 (2H, s), 6.29 (1H, s), 6.90-7.10 (3H, m), 7.30 (1H, t, J = 7.7 Hz), 7.50-7.60 (1H, m), 7.75-7.85 (1H, m), 7.85-7.95 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.29 (3H, t, J = 7.6 Hz), 2.75 (2H, q, J =7.6 Hz), 3.89 (3H, s), 5.37 (2H, s), 6.55-6.65 (1H, m), 6.95- 7.20 (3H, m), 7.25-7.55 (7H, m), 7.80-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 3.89 (3H, s), 5.55 (2H, s), 6.35-6.50 (2H, m), 6.78 (1H, s), 6.83 (1H, dd, J = 2.5, 8.8 Hz), 7.05-7.15 (3H, m), 7.25- 7.35 (1H, m), 7.40-7.50 (1H, m), 7.85-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 3.89 (3H, s), 5.42 (2H, s), 6.45-6.55 (1H, m), 6.55-6.65 (1H, m), 6.81 (1H, dd, J = 2.4, 8.9 Hz), 7.00-7.15 (3H, m), 7.33 (1H, t, J = 7.8 Hz), 7.40-7.50 (2H, m), 7.80-8.00 (2H, m).
1H-NMR (CDCl3) δ ppm: 0.65-0.75 (2H, m), 0.80- 0.95 (2H, m), 1.65-1.80 (1H, m), 3.83 (3H, s), 3.90 (3H, s), 5.47 (2H, s), 6.13 (1H, s), 6.75 (1H, dd, J = 2.4, 8.9 Hz), 6.95-7.10 (3H, m), 7.25-7.35 (1H, m), 7.85- 7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.89 (3H, s), 3.94 (3H, s), 5.32 (2H, s), 6.57 (1H, s), 6.86 (1H, d, J = 11.6 Hz), 7.05-7.15 (1H, m), 7.19 (1H, d, J = 8.3 Hz), 7.30- 7.50 (6H, m), 7.75-7.80 (1H, m), 7.85-8.00 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.89 (3H, s), 5.38 (2H, s), 6.50 (1H, t, J = 74.9 Hz), 6.64 (1H, s), 6.95 (1H, dd, J = 2.3, 8.8 Hz), 7.05- 7.15 (2H, m), 7.30-7.50 (7H, m), 7.75-8.00 (2H, m).
A suspension of methyl 3-{[2-(2,2-dibromovinyl)-4-ethoxyphenylamino]methyl}benzoate (258 mg), phenylboronic acid (134 mg), tris(dibenzylideneacetone)dipalladium (0) (25.1 mg), tris(2-methylphenyl)phosphine and potassium carbonate (381 mg) in toluene (5 mL) was stirred at 85° C. for 3.5 hours under an argon atmosphere. The reaction mixture was cooled to room temperature, diluted with ethyl acetate and filtered through celite (registered trademark). The filtrate was concentrated under reduced pressure. The residue was purified by aminopropylated silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (138 mg).
1H-NMR (CDCl3) δ ppm:
1.44 (3H, t, J=6.9 Hz), 3.88 (3H, s), 4.08 (2H, q, J=6.9 Hz), 5.36 (2H, s), 6.55-6.60 (1H, m), 6.80 (1H, dd, J=2.5, 8.8 Hz), 7.02 (1H, d, J=8.8 Hz), 7.05-7.45 (8H, m), 7.75-7.85 (1H, m), 7.85-7.95 (1H, m).
To a solution of 5-methoxy-2-phenylindole (90.9 mg) in N,N-dimethylformamide (1.8 mL) was added sodium hydride (dispersed in liquid paraffin, minimum 55%, 18 mg) under cooling with ice and an argon atmosphere, and the mixture was stirred under cooling with ice for 5 minutes and then at room temperature for 15 minutes. Methyl 5-(bromomethyl)-2-fluorobenzoate (101 mg) was added at room temperature, and the mixture was stirred at 80° C. for 13 hours. The reaction mixture was poured into a saturated aqueous ammonium chloride solution and extracted with ethyl acetate. The organic layer was washed with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography to obtain the title compound (53.2 mg).
In addition, structural formula and spectrum data of the title compound are shown in Table 13.
The compounds shown in Table 13 were synthesized in a manner similar to that of Example 5-1 by using the corresponding reactants.
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 3.90 (3H, s), 5.31 (2H, s), 6.55-6.60 (1H, m), 6.75-6.90 (1H, m), 6.90- 7.45 (9H, m), 7.65-7.70 (1H, m). ESI-MS (m/z): 390 (M + H)+
1H-NMR (CDCl3) δ ppm: 3.87 (3H, s), 3.89 (3H, s), 5.34 (2H, s), 6.55-6.65 (1H, m), 6.70-6.90 (2H, m), 7.02 (1H, d, J = 8.8 Hz), 7.14 (1H, d, J = 2.3 Hz), 7.30- 7.50 (5H, m), 7.50-7.65 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.87 (3H, s), 3.96 (3H, s), 5.41 (2H, s), 6.60-6.65 (1H, m), 6.65-6.75 (1H, m), 6.82 (1H, dd, J = 2.5, 8.8 Hz), 6.96 (1H, d, J = 8.8 Hz), 7.05-7.45 (7H, m), 7.60-7.70 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 3.89 (3H, s), 5.38 (2H, s), 6.60 (1H, s), 6.83 (1H, dd, J = 2.5, 8.8 Hz), 7.05 (1H, d, J = 8.8 Hz), 7.13 (1H, d, J = 2.5 Hz), 7.30-7.50 (5H, m), 7.80-7.95 (1H, m), 8.30- 8.45 (1H, m), 9.00-9.10 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.32 (3H, t, J = 7.1 Hz), 3.87 (3H, s), 4.29 (2H, q, J = 7.1 Hz), 5.42 (2H, s), 6.50-6.60 (1H, m), 6.65-6.75 (1H, m), 6.86 (1H, dd, J = 2.4, 8.9 Hz), 7.12 (1H, d, J = 2.4 Hz), 7.18 (1H, d, J = 8.9 Hz), 7.35-7.50 (5H, m), 7.58 (1H, d, J = 3.8 Hz).
1H-NMR (CDCl3) δ ppm: 3.87 (3H, s), 3.94 (3H, s), 5.41 (2H, s), 6.60 (1H, s), 6.70-6.90 (2H, m), 6.95- 7.10 (2H, m), 7.14 (1H, d, J = 2.5 Hz), 7.30-7.45 (5H, m), 7.75-7.85 (1H, m).
To a solution of 1-(3-hydroxybenzyl)-5-methoxy-2-phenylindole (108 mg) in N,N-dimethylformamide (1.3 mL) was added potassium carbonate (68.2 mg) at room temperature. Then ethyl bromoacetate (0.047 mL) was added, and the mixture was stirred at room temperature for 3 hours. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (129 mg).
1H-NMR (CDCl3) δ ppm:
1.25 (3H, t, J=7.2 Hz), 3.86 (3H, s), 4.20 (2H, q, J=7.2 Hz), 4.51 (2H, s), 5.29 (2H, s), 6.50-6.85 (5H, m), 7.05 (1H, d, J=8.8 Hz), 7.12 (1H, d, J=2.3 Hz), 7.15-7.25 (1H, m), 7.30-7.50 (5H, m).
To a suspension of methyl 3-{[2-(2-hydroxy-3,3-dimethylbutyl)-4-methoxyphenylamino]methyl}benzoate (200 mg), 2-bromomesitylene (0.097 mL) and potassium carbonate (148 mg) in N,N-dimethylformamide (5 mL) was added tetrakis(triphenylphosphine)palladium (0) (31.1 mg) at room temperature, and this resulting mixture was stirred at 150° C. for 2 hours under an argon atmosphere. The reaction mixture was cooled to room temperature, diluted with water and extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (127 mg).
1H-NMR (CDCl3) δ ppm:
1.40 (9H, s), 3.82 (3H, s), 3.90 (3H, s), 5.59 (2H, s), 6.30-6.40 (1H, m), 6.68 (1H, dd, J=2.4, 8.9 Hz), 6.75-6.90 (2H, m), 7.05 (1H, d, J=2.4 Hz), 7.20-7.35 (1H, m), 7.75-7.95 (2H, m).
The title compound was synthesized in a manner similar to that of Example 7 by using the corresponding starting material.
1H-NMR (CDCl3) δ ppm:
1.55-1.90 (6H, m), 1.90-2.10 (2H, m), 2.95-3.10 (1H, m), 3.83 (3H, s), 3.90 (3H, s), 5.37 (2H, s), 6.31 (1H, s), 6.72 (1H, dd, J=2.5, 8.8 Hz), 6.90-7.10 (3H, m), 7.20-7.35 (1H, m), 7.80-7.95 (2H, m).
To a solution of methyl 3-(5-methoxy-2-phenylindol-1-ylmethyl)benzoate (48.6 mg) in tetrahydrofuran-methanol (7/3, 1.1 mL) was added 2 mol/L aqueous sodium hydroxide solution (0.196 mL) at room temperature, and the mixture was stirred at 60° C. for 2 hours. 1 mol/L Hydrochloric acid (10 mL) was added to the reaction mixture at room temperature and this resulting mixture was extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure to obtain the title compound (44.7 mg).
In addition, structural formula and spectrum data of the title compound are shown in Table 14.
The compounds shown in Tables 14 to 22 were synthesized in a manner similar to that of Example 9-1 by using the corresponding starting materials.
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.50 (2H, s), 6.60 (1H, s), 6.76 (1H, dd, J = 2.5, 8.8 Hz), 7.05-7.15 (2H, m), 7.26 (1H, d, J = 8.8 Hz), 7.30- 7.55 (7H, m), 7.70-7.80 (1H, m), 12.95 (1H, s).
1H-NMR (DMSO-d6) δ ppm: 5.47 (2H, s), 6.20 (1H, d, J = 3.5 Hz), 6.62 (1H, s), 7.05 (1H, d, J = 3.5 Hz), 7.18 (1H, dd, J = 2.1, 8.7 Hz), 7.40-7.70 (7H, m), 12.60- 13.50 (1H, br).
1H-NMR (CDCl3) δ ppm: 5.52 (2H, s), 6.63 (1H, s), 6.85-7.25 (3H, m), 7.30-7.50 (5H, m), 7.55-7.90 (2H, m), 8.00-8.15 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.32 (6H, d, J = 6.8 Hz), 2.95-3.10 (1H, m), 5.34 (2H, s), 5.80-5.90 (1H, m), 6.30 (1H, s), 7.05-7.20 (3H, m), 7.45-7.55 (1H, m).
1H-NMR (DMSO-d6) δ ppm: 1.21 (6H, d, J = 6.8 Hz), 2.95-3.15 (1H, m), 5.55 (2H, s), 6.36 (1H, s), 7.02 (1H, dd, J = 2.0, 8.6 Hz), 7.10-7.20 (1H, m), 7.34 (1H, d, J = 8.6 Hz), 7.42 (1H, t, J = 7.7 Hz), 7.50-7.60 (2H, m), 7.75-7.85 (1H, m), 12.98 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.55 (2H, s), 6.68 (1H, s), 7.00-7.20 (2H, m), 7.30-7.55 (8H, m), 7.65-7.70 (1H, m), 7.70-7.80 (1H, m), 12.96 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.57 (2H, s), 6.76 (1H, s), 7.05-7.20 (2H, m), 7.30-7.65 (9H, m), 7.70-7.85 (1H, m), 12.98 (1H, s).
1H-NMR (DMSO-d6) δ ppm: 5.62 (2H, s), 6.87 (1H, s), 7.05-7.15 (1H, m), 7.37 (1H, t, J = 7.8 Hz), 7.40-7.55 (7H, m), 7.62 (1H, d, J = 8.8 Hz), 7.70-7.85 (1H, m), 8.04 (1H, s), 12.98 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.54 (2H, s), 6.65- 6.70 (1H, m), 6.90-7.05 (1H, m), 7.05-7.15 (1H, m), 7.30-7.60 (9H, m), 7.70-7.80 (1H, m), 12.97 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.62 (2H, s), 6.80- 6.90 (1H, m), 7.00-7.15 (1H, m), 7.37 (1H, t, J = 7.8 Hz), 7.40-7.55 (7H, m), 7.63 (1H, d, J = 8.5 Hz), 7.70-7.80 (1H, m), 8.15-8.20 (1H, m), 12.98 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.77 (3H, t, J = 7.3 Hz), 1.17 (3H, d, J = 6.8 Hz), 1.40-1.75 (2H, m), 2.75-2.90 (1H, m), 5.54 (2H, s), 6.34 (1H, s), 7.02 (1H, dd, J = 2.0, 8.5 Hz), 7.10-7.20 (1H, m), 7.30- 7.60 (4H, m), 7.75-7.85 (1H, m), 12.97 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.89 (6H, d, J = 6.5 Hz), 1.75-1.95 (1H, m), 2.57 (2H, d, J = 7.0 Hz), 5.51 (2H, s), 6.34 (1H, s), 7.02 (1H, dd, J = 2.0, 8.7 Hz), 7.10-7.20 (1H, m), 7.30-7.60 (4H, m), 7.75- 7.85 (1H, m), 12.98 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 2.38 (3H, s), 5.50 (2H, s), 6.55-6.60 (1H, m), 6.90-7.00 (1H, m), 7.05-7.15 (1H, m), 7.24 (1H, d, J = 8.3 Hz), 7.30-7.55 (8H, m), 7.70- 7.80 (1H, m), 12.91 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.45 (2H, s), 6.21 (1H, d, J = 3.5 Hz), 6.62 (1H, s), 6.95-7.10 (2H, m), 7.36 (1H, dd, J = 2.5, 9.8 Hz), 7.40-7.65 (6H, m), 13.04 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 1.20 (6H, d, J = 6.8 Hz), 2.90-3.10 (1H, m), 3.73 (3H, s), 5.47 (2H, s), 6.26 (1H, s), 6.65 (1H, dd, J = 2.4, 8.8 Hz), 7.01 (1H, d, J = 2.4 Hz), 7.10-7.20 (2H, m), 7.40 (1H, t, J = 7.7 Hz), 7.50- 7.60 (1H, m), 7.75-7.85 (1H, m), 12.93 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.56 (2H, s), 6.69 (1H, s), 6.75-6.85 (1H, m), 6.90-7.05 (1H, m), 7.30-7.65 (7H, m), 7.80- 7.95 (2H, m), 12.50-13.80 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 1.34 (3H, t, J = 7.0 Hz), 4.02 (2H, q, J = 7.0 Hz), 5.49 (2H, s), 6.58 (1H, s), 6.75 (1H, dd, J = 2.4, 9.0 Hz), 7.05- 7.15 (2H, m), 7.24 (1H, d, J = 9.0 Hz), 7.30-7.55 (7H, m), 7.70-7.80 (1H, m), 12.94 (1H, s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.41 (2H, s), 6.18 (1H, d, J = 3.5 Hz), 6.54 (1H, s), 6.81 (1H, dd, J = 2.5, 9.0 Hz), 7.07 (1H, d, J = 3.5 Hz), 7.09 (1H, d, J = 2.5 Hz), 7.40-7.65 (6H, m), 13.04 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.78 (3H, t, J = 7.3 Hz), 1.17 (3H, d, J = 6.8 Hz), 1.40-1.75 (2H, m), 2.70-2.85 (1H, m), 3.74 (3H, s), 5.47 (2H, s), 6.24 (1H, s), 6.65 (1H, dd, J = 2.5, 8.8 Hz), 7.01 (1H, d, J = 2.5 Hz), 7.10-7.25 (2H, m), 7.40 (1H, t, J = 7.7 Hz), 7.50-7.55 (1H, m), 7.75-7.85 (1H, m), 12.97 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.89 (6H, d, J = 6.5 Hz), 1.70-1.95 (1H, m), 2.54 (2H, d, J = 7.0 Hz), 3.74 (3H, s), 5.44 (2H, s), 6.24 (1H, s), 6.64 (1H, dd, J = 2.4, 8.9 Hz), 7.01 (1H, d, J = 2.4 Hz), 7.10-7.25 (2H, m), 7.41 (1H, t, J = 7.7 Hz), 7.45-7.55 (1H, m), 7.75-7.85 (1H, m), 12.96 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.53 (2H, s), 6.62 (1H, s), 6.65-6.80 (2H, m), 7.13 (1H, d, J = 2.3 Hz), 7.24 (1H, d, J = 9.0 Hz), 7.30-7.50 (3H, m), 7.50-7.65 (2H, m), 7.75-7.95 (2H, m), 12.85-13.65 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 0.71 (6H, t, J = 7.4 Hz), 1.45-1.70 (4H, m), 2.55-2.70 (1H, m), 3.74 (3H, s), 5.47 (2H, s), 6.22 (1H, s), 6.66 (1H, dd, J = 2.3, 8.9 Hz), 7.01 (1H, d, J = 2.3 Hz), 7.15-7.30 (2H, m), 7.40 (1H, t, J = 7.7 Hz), 7.45-7.60 (1H, m), 7.70- 7.85 (1H, m), 12.93 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.45 (2H, s), 6.59 (1H, s), 6.77 (1H, dd, J = 2.4, 8.9 Hz), 7.00-7.60 (10H, m), 13.23 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.51 (2H, s), 6.62 (1H, s), 6.78 (1H, dd, J = 2.5, 8.8 Hz), 6.90-7.00 (1H, m), 7.13 (1H, d, J = 2.5 Hz), 7.25- 7.35 (2H, m), 7.35-7.55 (6H, m), 13.29 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 5.58 (2H, s), 6.66 (1H, s), 6.75 (1H, dd, J = 2.5, 9.0 Hz), 7.09 (1H, d, J = 2.5 Hz), 7.10-7.20 (1H, m), 7.20- 7.35 (2H, m), 7.39 (1H, t, J = 7.8 Hz), 7.50-7.65 (2H, m), 7.66 (1H, dd, J = 3.0, 5.0 Hz), 7.70-7.80 (1H, m), 12.96 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.70 (6H, t, J = 7.3 Hz), 1.45- 1.70 (4H, m), 2.60-2.75 (1H, m), 5.54 (2H, s), 6.32 (1H, s), 7.03 (1H, dd, J = 2.1, 8.7 Hz), 7.15-7.25 (1H, m), 7.35-7.60 (4H, m), 7.75-7.85 (1H, m), 12.95 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.78 (3H, s), 5.48 (2H, s), 6.25-6.40 (1H, m), 6.60-6.70 (1H, m), 6.77 (1H, dd, J = 2.5, 8.8 Hz), 7.10-7.30 (3H, m), 7.35-7.50 (5H, m), 7.50-7.60 (1H, m), 13.50 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.57 (2H, s), 6.61 (1H, s), 6.79 (1H, dd, J = 2.4, 8.9 Hz), 7.12 (1H, d, J = 2.4 Hz), 7.35-7.55 (6H, m), 7.60- 7.70 (1H, m), 8.25-7.35 (1H, m), 8.80-8.90 (1H, m), 13.42 (1H, s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.61 (2H, s), 6.57 (1H, s), 6.75-6.85 (2H, m), 7.10 (1H, d, J = 2.5 Hz), 7.35- 7.60 (7H, m), 12.80-13.20 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 1.22 (3H, t, J = 7.6 Hz), 2.67 (2H, q, J = 7.6 Hz), 5.50 (2H, s), 6.61 (1H, s), 6.95-7.05 (1H, m), 7.05-7.15 (1H, m), 7.26 (1H, d, J = 8.3 Hz), 7.30-7.55 (8H, m), 7.70- 7.80 (1H, m), 12.95 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.69 (2H, s), 6.55-6.70 (2H, m), 6.75-6.85 (2H, m), 7.11 (1H, d, J = 2.5 Hz), 7.15-7.25 (1H, m), 7.30-7.50 (2H, m), 7.50-7.60 (1H, m), 7.70-7.85 (2H, m), 12.96 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 5.60 (2H, s), 6.67 (1H, s), 6.70-6.85 (2H, m), 7.08 (1H, d, J = 2.5 Hz), 7.10-7.20 (1H, m), 7.32 (1H, d, J = 9.0 Hz), 7.39 (1H, t, J = 7.7 Hz), 7.50-7.55 (1H, m), 7.70-7.80 (2H, m), 7.85- 7.95 (1H, m), 12.96 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 4.54 (2H, s), 5.39 (2H, s), 6.40-6.55 (2H, m), 6.57 (1H, s), 6.55-6.80 (2H, m), 7.05-7.20 (2H, m), 7.24 (1H, d, J = 9.0 Hz), 7.35-7.55 (5H, m), 12.99 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 1.22 (3H, t, J = 7.6 Hz), 2.67 (2H, q, J = 7.6 Hz), 5.52 (2H, s), 6.55-6.70 (2H, m), 6.90-7.05 (1H, m), 7.21 (1H, d, J = 8.3 Hz), 7.35- 7.50 (4H, m), 7.50-7.60 (2H, m), 7.70-7.90 (2H, m), ESI-MS (m/z): 355 (M − H)−
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 5.59 (2H, s), 6.67 (1H, s), 6.70-6.85 (2H, m), 7.10 (1H, d, J = 2.5 Hz), 7.28 (1H, d, J = 8.8 Hz), 7.37 (1H, dd, J = 1.3, 5.0 Hz), 7.65 (1H, dd, J = 2.9, 5.0 Hz), 7.77 (1H, dd, J = 1.3, 2.9 Hz), 7.80-7.95 (2H, m), 13.24 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 5.48 (2H, s), 6.25 (1H, d, J = 3.3 Hz), 6.55-6.60 (1H, m), 6.79 (1H, dd, J = 2.5, 8.8 Hz), 7.00-7.15 (2H, m), 7.35- 7.50 (2H, m), 7.72 (1H, dd, J = 2.9, 4.9 Hz), 7.76 (1H, dd, J = 1.4, 2.9 Hz), 13.03 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 1.35 (9H, s), 3.72 (3H, s), 5.68 (2H, s), 6.28 (1H, s), 6.60 (1H, dd, J = 2.5, 8.8 Hz), 6.92 (1H, d, J = 8.8 Hz), 6.95-7.05 (2H, m), 7.37 (1H, t, J = 7.8 Hz), 7.45-7.55 (1H, m), 7.70-7.80 (1H, m), 12.90 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 1.50-1.80 (6H, m), 1.85- 2.00 (2H, m), 3.05-3.15 (1H, m), 3.73 (3H, s), 5.48 (2H, s), 6.27 (1H, s), 6.64 (1H, dd, J = 2.4, 8.7 Hz), 7.00 (1H, d, J = 2.4 Hz), 7.10-7.20 (2H, m), 7.40 (1H, t, J = 7.7 Hz), 7.50-7.60 (1H, m), 7.75-7.85 (1H, m), 12.95 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.55-0.70 (2H, m), 0.80-0.95 (2H, m), 1.80-1.95 (1H, m), 3.72 (3H, s), 5.56 (2H, s), 6.08 (1H, s), 6.66 (1H, dd, J = 2.4, 8.8 Hz), 6.96 (1H, d, J = 2.4 Hz), 7.15-7.30 (2H, m), 7.42 (1H, t, J = 7.7 Hz), 7.55-7.65 (1H, m), 7.75-7.85 (1H, m), 12.94 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 1.27 (6H, d, J = 6.8 Hz), 3.10-3.30 (1H, m), 3.72 (3H, s), 5.43 (2H, s), 6.20 (1H, s), 6.33 (1H, d, J = 3.4 Hz), 6.69 (1H, dd, J = 2.4, 8.9 Hz), 6.97 (1H, d, J = 2.4 Hz), 7.09 (1H, d, J = 3.4 Hz), 7.36 (1H, d, J = 8.9 Hz), 13.00 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 1.22 (6H, d, J = 6.8 Hz), 3.00-3.20 (1H, m), 3.73 (3H, s), 5.52 (2H, s), 6.27 (1H, s), 6.55-6.75 (2H, m), 7.01 (1H, d, J = 2.5 Hz), 7.20 (1H, d, J = 8.8 Hz), 7.75-7.95 (2H, m), 13.23 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.57 (2H, s), 6.72 (1H, s), 6.75-6.85 (1H, m), 6.90-7.35 (2H, m), 7.35-7.55 (5H, m), 7.55- 7.65 (2H, m), 7.75-7.95 (2H, m), 12.80-13.65 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.85 (3H, s), 5.49 (2H, s), 6.62 (1H, s), 7.00-7.10 (1H, m), 7.25- 7.55 (9H, m), 7.70-7.80 (1H, m), 12.90 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.50 (2H, s), 6.50-6.60 (1H, m), 6.60 (1H, s), 6.78 (1H, dd, J = 2.3, 8.9 Hz), 7.07 (1H, t, J = 7.8 Hz), 7.13 (1H, d, J = 2.3 Hz), 7.30 (1H, d, J = 8.9 Hz), 7.35-7.50 (5H, m), 7.60- 7.75 (1H, m), 13.27 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.90 (6H, d, J = 6.5 Hz), 1.75-1.95 (1H, m), 2.59 (2H, d, J = 7.0 Hz), 3.74 (3H, s), 5.50 (2H, s), 6.25 (1H, s), 6.55-6.75 (2H, m), 7.02 (1H, d, J = 2.3 Hz), 7.21 (1H, d, J = 8.8 Hz), 7.75-7.95 (2H, m), 13.24 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.55 (2H, s), 6.71 (1H, s), 6.90-7.60 (12H, m), 7.70-7.85 (1H, m), 12.94 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.95 (6H, d, J = 6.5 Hz), 1.85-2.05 (1H, m), 2.66 (2H, d, J = 7.3 Hz), 3.73 (3H, s), 5.40 (2H, s), 6.18 (1H, s), 6.32 (1H, d, J = 3.3 Hz), 6.69 (1H, dd, J = 2.4, 8.9 Hz), 6.97 (1H, d, J = 2.4 Hz), 7.09 (1H, d, J = 3.3 Hz), 7.36 (1H, d, J = 8.9 Hz), 13.00 (1H, br s).
The compounds shown in Tables 23 to 25 were synthesized in a manner similar to that of Example 1-1 by using the corresponding starting materials.
1H-NMR (CDCl3) δ ppm: 2.45 (3H, s), 4.02 (3H, s), 5.59 (2H, s), 6.61 (1H, s), 6.70 (1H, d, J = 8.0 Hz), 6.90-7.10 (2H, m), 7.30-7.55 (6H, m), 7.80-7.70 (1H, m), 7.98 (1H, d, J = 7.5 Hz).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 4.05 (3H, s), 5.62 (2H, s), 6.45-6.55 (1H, m), 6.62 (1H, s), 6.65-6.75 (1H, m), 6.82 (1H, dd, J = 2.3, 8.9 Hz), 7.06 (1H, d, J = 8.9 Hz), 7.12 (1H, d, J = 2.3 Hz), 7.40-7.55 (2H, m), 7.60-7.70 (1H, m), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 0.85 (t, 3H, J = 7.4 Hz), 1.23 (3H, d, J = 6.8 Hz), 1.45-1.80 (2H, m), 2.60-2.80 (1H, m), 3.84 (3H, s), 4.05 (3H, s), 5.54 (2H, s), 6.32 (1H, s), 6.40-6.50 (1H, m), 6.75 (1H, dd, J = 2.3, 8.8 Hz), 7.00 (1H, d, J = 8.8 Hz), 7.08 (1H, d, J = 2.3 Hz), 7.61 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.76 (3H, s), 3.86 (3H, s), 5.54 (2H, s), 6.67 (1H, s), 6.77 (1H, dd, J = 2.4, 8.8 Hz), 6.85-6.95 (1H, m), 7.13 (1H, d, J = 2.4 Hz), 7.15-7.35 (2H, m), 7.35-7.60 (3H, m), 7.80-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 4.03 (3H, s), 5.64 (2H, s), 6.60-6.80 (2H, m), 7.21 (1H, d, J = 8.3 Hz), 7.30-7.50 (6H, m), 7.69 (1H, t, J = 7.8 Hz), 7.95-8.05 (2H, m).
1H-NMR (CDCl3) δ ppm: 4.02 (3H, s), 5.59 (2H, s), 6.60-6.70 (2H, m), 7.00 (1H, d, J = 8.7 Hz), 7.22 (1H, dd, J = 1.8, 8.7 Hz), 7.30-7.50 (5H, m), 7.60-7.75 (1H, m), 7.81 (1H, d, J = 1.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 2.52 (3H, s), 4.02 (3H, s), 5.59 (2H, s), 6.60-6.75 (2H, m), 7.06 (1H, d, J = 8.5 Hz), 7.16 (1H, dd, J = 1.8, 8.5 Hz), 7.30-7.45 (5H, m), 7.60-7.70 (2H, m), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.94 (3H, s), 4.03 (3H, s), 5.53 (2H, s), 6.55-6.75 (2H, m), 6.80-6.95 (1H, m), 7.20 (1H, d, J = 8.3 Hz), 7.30-7.45 (5H, m), 7.68 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 4.05 (3H, s), 5.83 (2H, s), 6.75-6.90 (3H, m), 7.05-7.20 (2H, m), 7.60-7.70 (1H, m), 7.80-7.95 (2H. m), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm 3.86 (3H, s), 4.01 (3H, s), 5.47 (2H, s), 6.60-6.70 (2H, m), 6.82 (1H, dd, J = 2.4, 8.9 Hz), 7.01 (1H, d, J = 8.9 Hz), 7.05-7.20 (3H, m), 7.30-7.45 (2H, m), 7.55-7.70 (1H, m), 7.90-8.00 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 4.03 (3H, s), 5.54 (2H, s), 6.50-6.75 (2H, m), 6.82 (1H, dd, J = 2.5, 8.8 Hz), 6.95-7.20 (4H, m), 7.30-7.45 (2H, m), 7.60-7.75 (1H, m), 7.90-8.10 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.44 (3H, t, J = 7.0 Hz), 4.02 (3H, s), 4.09 (2H, q, J = 7.0 Hz), 5.58 (2H, s), 6.55-6.65 (1H, m), 6.65-6.75 (1H, m), 6.80 (1H, dd, J = 2.4, 8.9 Hz), 7.01 (1H, d, J = 8.9 Hz), 7.10-7.20 (1H, m), 7.30-7.45 (5H, m), 7.66 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
The compounds shown in Tables 26 to 27 were synthesized in a manner similar to that of Example 2-1 by using the corresponding starting materials.
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 2.46 (3H, s), 4.34 (2H, q, J = 7.1 Hz), 5.30 (2H, s), 5.97 (1H, d, J = 3.5 Hz), 6.54 (1H, s), 7.00-7.10 (2H, m), 7.18 (1H, d, J = 8.3 Hz), 7.35-7.55 (6H, m).
1H-NMR (CDCl3) δ ppm: 0.91 (3H, t, J = 7.4 Hz), 1.28 (3H, d, J = 6.8 Hz), 1.36 (3H, t, J = 7.1 Hz), 1.50-1.85 (2H, m), 2.75-2.90 (1H, m), 3.83 (3H, s), 4.35 (2H, q, J = 7.1 Hz), 5.30 (2H, s), 5.84 (1H, d, J = 3.5 Hz), 6.25 (1H, s), 6.78 (1H, dd, J = 2.4, 8.8 Hz), 7.00 (1H, d, J = 3.5 Hz), 7.04 (1H, d, J = 2.4 Hz), 7.11 (1H, d, J = 8.8 Hz).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 4.35 (2H, q, J = 7.1 Hz), 5.35 (2H, s), 5.99 (1H, d, J = 3.5 Hz), 6.69 (1H, s), 7.05 (1H, d, J = 3.5 Hz), 7.35-7.55 (7H, m), 7.94 (1H, s).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 3.86 (3H, s), 4.36 (2H, q, J = 7.1 Hz), 5.34 (2H, s), 5.95-6.05 (1H, m), 6.50-6.65 (2H, m), 6.86 (1H, dd, J = 2.5, 8.9 Hz), 7.05 (1H, d, J = 3.5 Hz), 7.08 (1H, d, J = 2.5 Hz), 7.18 (1H, d, J = 8.9 Hz), 7.45-7.55 (1H, m), 7.55-7.65 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 4.34 (2H, q, J = 7.1 Hz), 5.30 (2H, s), 5.97 (1H, d, J = 3.5 Hz), 6.50-6.60 (1H, m), 7.04 (1H, d, J = 3.5 Hz), 7.18 (1H, d, J = 8.7 Hz), 7.29 (1H, dd, J = 1.9, 8.7 Hz), 7.35-7.55 (5H, m), 7.75-7.80 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 3.94 (3H, s), 4.35 (2H, q, J = 7.1 Hz), 5.24 (2H, s), 5.95-6.05 (1H, m), 6.50-6.55 (1H, m), 7.00-7.10 (2H, m), 7.17 (1H, d, J = 8.3 Hz), 7.35-7.55 (5H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.2 Hz), 3.86 (3H, s), 4.34 (2H, q, J = 7.2 Hz), 5.25 (2H, s), 5.95-6.05 (1H, m), 6.45-6.55 (1H, m), 6.87 (1H, dd, J = 2.5, 9.0 Hz), 7.00-7.25 (5H, m), 7.40-7.50 (2H, m).
The compounds shown in Table 28 were synthesized in a manner similar to that of Example 3-1 by using the corresponding starting materials.
1H-NMR (CDCl3) δ ppm: 3.88 (3H, s), 5.37 (2H, s), 6.59 (1H, s), 6.95-7.15 (2H, m), 7.22 (1H, dd, J = 1.9, 8.7 Hz), 7.25-7.50 (6H, m), 7.70-7.85 (2H, m), 7.85-8.00 (1H, m).
1H-NMR (CDCl3) δ ppm: 2.52 (3H, s), 3.89 (3H, s), 5.37 (2H, s), 6.55-6.65 (1H, m), 7.00-7.15 (2H, m), 7.16 (1H, dd, J = 1.8, 8.5 Hz), 7.25-7.50 (6H, m), 7.60-7.70 (1H, m), 7.75-7.85 (1H, m), 7.85-7.95 (1H, m).
To a solution of 2-furan-3-yl-5-methoxyindole (200 mg) in N,N-dimethylformamide (4.7 mL) was added sodium hydride (dispersed in liquid paraffin, minimum 55%, 62 mg) under cooling with ice, and the mixture was stirred at room temperature for one hour. Then a solution of methyl 3-bromomethyl-2-fluorobenzoate (278 mg) in N,N-dimethylformamide (0.2 mL) was added, and the mixture was stirred at 80° C. for 15 hours. The reaction mixture was allowed to cool to ambient temperature. A saturated aqueous ammonium chloride solution-water (2/1) were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by aminopropylated silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (72 mg).
1H-NMR (CDCl3) δ ppm:
3.86 (3H, s), 3.96 (3H, s), 5.45 (2H, s), 6.40-6.50 (1H, m), 6.55-6.75 (2H, m), 6.83 (1H, dd, J=2.4, 8.9 Hz), 6.95-7.15 (3H, m), 7.40-7.50 (2H, m), 7.75-7.85 (1H, m).
The title compound was synthesized in a manner similar to that of Example 5-1 by using the corresponding reactant.
1H-NMR (CDCl3) δ ppm:
3.81 (3H, s), 3.86 (3H, s), 5.35-5.45 (2H, m), 6.50-6.60 (1H, m), 6.86 (1H, dd, J=2.5, 8.8 Hz), 7.05-7.15 (1H, m), 7.15-7.25 (2H, m), 7.35-7.55 (5H, m), 7.91 (1H, d, J=1.5 Hz).
To a solution of 6-fluoro-5-methoxy-2-phenylindole (200 mg) in N,N-dimethylformamide (4.1 mL) was added sodium hydride (dispersed in liquid paraffin, minimum 55%, 54 mg) under cooling with ice, and the mixture was stirred at room temperature for 70 minutes. Then a solution of ethyl 2-bromomethylthiazole-4-carboxylate (249 mg) in N,N-dimethylformamide (0.2 mL) was added, and the mixture was stirred at 80° C. for 25 hours. The reaction mixture was allowed to cool to ambient temperature. A saturated aqueous ammonium chloride solution-water (2/1) were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (125 mg).
In addition, structural formula and spectrum data of the title compound are shown in Table 29.
The compounds shown in Table 29 were synthesized in a manner similar to that of Example 15-1 by using the corresponding starting materials.
1H-NMR (CDCl3) δ ppm: 1.41 (3H, t, J = 7.1 Hz), 3.94 (3H, s), 4.43 (2H, q, J = 7.1 Hz), 5.58 (2H, s), 6.60 (1H, s), 7.00 (1H, d, J = 11.3 Hz), 7.18 (1H, d, J = 8.3 Hz), 7.35-7.50 (5H, m), 8.03 (1H, s).
1H-NMR (CDCl3) δ ppm: 1.40 (3H, t, J = 7.1 Hz), 3.86 (3H, s), 4.42 (2H, q, J = 7.1 Hz), 5.62 (2H, s), 6.55-6.65 (1H, m), 6.85 (1H, dd, J = 2.5, 8.8 Hz), 7.10-7.20 (2H, m), 7.35-7.55 (5H, m), 8.02 (1H, s).
1H-NMR (CDCl3) δ ppm: 0.89 (3H, t, J = 7.4 Hz), 1.27 (3H, d, J = 6.8 Hz), 1.42 (3H, t, J = 7.1 Hz), 1.50-1.85 (2H, m), 2.70-2.85 (1H, m), 3.84 (3H, s), 4.45 (2H, q, J = 7.1 Hz), 5.60 (2H, s), 6.31 (1H, s), 6.79 (1H, dd, J = 2.4, 8.8 Hz), 7.06 (1H, d, J = 2.4 Hz), 7.10 (1H, d, J = 8.8 Hz), 8.01 (1H, s).
1H-NMR (CDCl3) δ ppm: 1.42 (3H, t, J = 7.1 Hz), 3.86 (3H, s), 4.45 (2H, q, J = 7.1 Hz), 5.66 (2H, s), 6.50-6.65 (2H, m), 6.86 (1H, dd, J = 2.4, 9.0 Hz), 7.05-7.15 (1H, m), 7.16 (1H, d, J = 9.0 Hz), 7.45-7.60 (2H, m), 8.04 (1H, s).
The compounds shown in Tables 30 to 34 were synthesized in a manner similar to that of Example 9-1 by using the corresponding starting materials.
1H-NMR (DMSO-d6) δ ppm: 2.38 (3H, s), 5.41 (2H, s), 6.16 (1H, d, J = 3.5 Hz), 6.53 (1H, s), 6.95-7.05 (1H, m), 7.06 (1H, d, J = 3.5 Hz), 7.30-7.70 (7H, m), 13.01 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 2.38 (3H, s), 5.53 (2H, s), 6.61 (1H, s), 6.65-6.75 (1H, m), 6.90-7.00 (1H, m), 7.21 (1H, d, J = 8.5 Hz), 7.30-7.65 (6H, m), 7.75-7.95 (2H, m), 12.50-13.90 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 0.85 (3H, t, J = 7.4 Hz), 1.23 (3H, d, J = 6.8 Hz), 1.45-1.80 (2H, m), 2.95-3.10 (1H, m), 3.73 (3H, s), 5.43 (2H, s), 6.18 (1H, s), 6.34 (1H, d, J = 3.5 Hz), 6.69 (1H, dd, J = 2.3, 8.8 Hz), 6.97 (1H, d, J = 2.3 Hz), 7.09 (1H, d, J = 3.5 Hz), 7.37 (1H, d, J = 8.8 Hz), 12.99 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 5.60 (2H, s), 6.66 (1H, s), 6.70-6.90 (3H, m), 7.09 (1H, d, J = 2.5 Hz), 7.35 (1H, d, J = 9.0 Hz), 7.70-8.10 (4H, m), 12.50-14.00 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 0.79 (3H, t, J = 7.4 Hz), 1.18 (3H, d, J = 6.8 Hz), 1.40-1.75 (2H, m), 2.80-2.95 (1H, m), 3.73 (3H, s), 5.45-5.60 (2H, m), 6.25 (1H, s), 6.55-6.75 (2H, m), 7.01 (1H, d, J = 2.5 Hz), 7.23 (1H, d, J = 9.0 Hz), 7.75-7.95 (2H, m), 12.50-14.00 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 5.54 (2H, s), 6.22 (1H, d, J = 3.5 Hz), 6.80 (1H, s), 7.06 (1H, d, J = 3.5 Hz), 7.40-7.70 (6H, m), 7.79 (1H, d, J = 8.5 Hz), 8.01 (1H, br s), 13.05 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.55 (2H, s), 6.68 (1H, s), 7.00-7.15 (1H, m), 7.24 (1H, dd, J = 2.0, 8.8 Hz), 7.30-7.60 (8H, m), 7.70-7.90 (2H, m), 12.94 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.53 (2H, s), 6.65-6.70 (1H, m), 6.77 (1H, dd, J = 2.4, 9.0 Hz), 6.80-6.90 (1H, m), 7.10-7.15 (1H, m), 7.20-7.30 (2H, m), 7.40-7.55 (3H, m), 7.80-7.90 (2H, m). ESI-MS (m/z): 377 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 5.63 (2H, s), 6.80-6.95 (2H, m), 7.35-7.70 (7H, m), 7.80-7.95 (2H, m), 8.00-8.10 (1H, m), 13.21 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 5.49 (2H, s), 6.32 (1H, d, J = 3.4 Hz), 6.58 (1H, s), 6.79 (1H, dd, J = 2.4, 9.0 Hz), 6.85-6.95 (1H, m), 7.05 (1H, d, J = 2.4 Hz), 7.09 (1H, d, J = 3.4 Hz), 7.47 (1H, d, J = 9.0 Hz), 7.80-7.90 (1H, m), 8.00-8.10 (1H, m), 13.04 (1H, s).
1H-NMR (DMSO-d6) δ ppm: 5.57 (2H, s), 6.69 (1H, s), 6.75-6.85 (1H, m), 7.23 (H, dd, J = 1.9, 8.7 Hz), 7.30-7.65 (6H, m), 7.80-7.95 (3H, m), 13.21 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 5.47 (2H, s), 6.20 (1H, d, J = 3.4 Hz), 6.62 (1H, s), 7.06 (1H, d, J = 3.4 Hz), 7.30 (1H, dd, J = 1.9, 8.8 Hz), 7.40-7.70 (6H, m), 7.79 (1H, d, J = 1.9 Hz), 12.60-13.45 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 2.48 (3H, s), 5.55 (2H, s), 6.60-6.70 (1H, m), 6.75-6.85 (1H, m), 7.09 (1H, dd, J = 1.8, 8.5 Hz), 7.31 (1H, d, J = 8.5 Hz), 7.35-7.65 (6H, m), 7.80-7.95 (2H, m). ESI-MS (m/z): 375 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 2.48 (3H, s), 5.52 (2H, s), 6.60-6.70 (1H, m), 7.05-7.15 (2H, m), 7.25-7.65 (9H, m), 7.70-7.80 (1H, m), 12.93 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.85 (3H, s), 5.52 (2H, s), 6.60-6.80 (2H, m), 7.25-7.65 (7H, m), 7.75-7.95 (2H, m), 11.90-14.50 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.85 (3H, s), 5.41 (2H, s), 6.18 (1H, d, J = 3.5 Hz), 6.50-6.60 (1H, m), 7.06 (1H, d, J = 3.5 Hz), 7.28 (1H, d, J = 8.5 Hz), 7.40-7.65 (6H, m), 13.02 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.78 (3H, s), 5.72 (2H, s), 6.60-6.70 (1H, m), 6.82 (1H, dd, J = 2.5, 9.0 Hz), 7.10-7.20 (1H, m), 7.35-7.65 (6H, m), 8.25 (1H, s), 12.00-14.00 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.82 (2H, s), 6.65-6.75 (1H, m), 6.78 (1H, dd, J = 2.5, 8.8 Hz), 6.85 (1H, s), 7.13 (1H, d, J = 2.5 Hz), 7.35 (1H, d, J = 8.8 Hz), 7.75-7.95 (2H, m), 8.45-8.65 (2H, m), 12.00-14.40 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.86 (3H, s), 5.73 (2H, s), 6.60-6.70 (1H, m), 7.32 (1H, d, J = 8.5 Hz), 7.35-7.60 (6H, m), 8.25 (1H, s), 13.01 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.42 (2H, s), 6.55-6.70 (2H, m), 6.79 (1H, dd, J = 2.5, 8.8 Hz), 7.15 (1H, d, J = 2.5 Hz), 7.20-7.40 (3H, m), 7.40-7.60 (2H, m), 7.75-7.90 (2H, m), 12.60-13.80 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.59 (2H, s), 6.40-6.55 (1H, m), 6.60-6.80 (3H, m), 7.00-7.15 (2H, m), 7.32 (1H, d, J = 9.0 Hz), 7.65-7.80 (2H, m), 7.85-7.95 (1H, m), 13.32 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.50 (2H, s), 6.60 (1H, s), 6.70-6.85 (2H, m), 7.12 (1H, d, J = 2.3 Hz), 7.20-7.35 (3H, m), 7.55-7.70 (2H, m), 7.75-795 (2H, m), 13.23 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.81 (3H, t, J = 7.3 Hz), 1.20 (3H, d, J = 6.8 Hz), 1.45-1.75 (2H, m), 2.85-3.00 (1H, m), 3.74 (3H, s), 5.72 (2H, s), 6.25 (1H, s), 6.71 (1H, dd, J = 2.4, 8.9 Hz), 7.01 (1H, d, J = 2.4 Hz), 7.33 (1H, d, J = 8.9 Hz), 8.28 (1H, s), 13.04 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.58 (2H, s), 6.56 (1H, s), 6.81 (1H, dd, J = 2.4, 8.8 Hz), 6.95-7.05 (1H, m), 7.09 (1H, d, J = 2.4 Hz), 7.40-7.60 (6H, m), 7.98 (1H, d, J = 1.5 Hz), 12.66 (2H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 5.39 (2H, s), 6.20 (1H, d, J = 3.4 Hz), 6.53 (1H, s), 6.81 (1H, dd, J = 2.4, 9.0 Hz), 7.06 (1H, d, J = 3.4 Hz), 7.09 (1H, d, J = 2.4 Hz), 7.25-7.40 (2H, m), 7.45 (1H, d, J = 9.0 Hz), 7.55-7.70 (2H, m), 13.03 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.82 (2H, s), 6.68 (1H, s), 6.75-6.90 (2H, m), 7.08 (1H, d, J = 2.3 Hz), 7.48 (1H, d, J = 8.8 Hz), 7.75-7.85 (1H, m), 8.00-8.10 (1H, m), 8.27 (1H, s), 13.07 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 1.34 (3H, t, J = 6.9 Hz), 4.02 (2H, q, J = 6.9 Hz), 5.52 (2H, s), 6.60 (1H, s), 6.65-6.80 (2H, m), 7.12 (1H, d, J = 2.5 Hz), 7.22 (1H, d, J = 8.8 Hz), 7.35-7.50 (3H, m), 7.50-7.65 (2H, m), 7.75-7.95 (2H, m), 12.00-14.40 (1H, br).
The compounds shown in Tables 35 to 38 were synthesized in a manner similar to that of Example 1-1 by using the corresponding starting materials.
The compounds shown in Tables 39 to 40 were synthesized in a manner similar to that of Example 2-1 by using the corresponding starting materials.
The compounds shown in Tables 41 to 42 were synthesized in a manner similar to that of Example 3-1 by using the corresponding starting materials.
The title compound was synthesized in a manner similar to that of Example 5-1 by using the corresponding reactant.
1H-NMR (CDCl3) δ ppm:
1.44 (3H, t, J=7.1 Hz), 3.87 (3H, s), 4.49 (2H, q, J=7.1 Hz), 5.56 (2H, s), 6.60 (1H, s), 6.78 (1H, s), 6.84 (1H, d, J=2.4, 8.9 Hz), 7.08 (1H, d, J=8.9 Hz), 7.14 (1H, d, J=2.4 Hz), 7.30-7.50 (5H, m).
To a solution of 5-methoxy-2-phenylindole (205 mg) in N,N-dimethylformamide (4.6 mL) was added sodium hydride (in oil, 50 to 72%, 55 mg) under cooling with ice and an argon atmosphere, and the mixture was stirred at room temperature for 2 hours. Then methyl 2-(chloromethyl)-1,3-oxazole-4-carboxylate (193 mg) was added, and the mixture was stirred at 80° C. for 22 hours. The reaction mixture was allowed to cool to ambient temperature. A saturated aqueous ammonium chloride solution and water were added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (229 mg).
In addition, structural formula and spectrum data of the title compound are shown in Table 42.
1H-NMR (CDCl3) δ ppm:
3.85 (3H, s), 3.92 (3H, s), 5.39 (2H, s), 6.55 (1H, s), 6.87 (1H, dd, J=2.5, 8.8 Hz), 7.10 (1H, d, J=2.5 Hz), 7.30 (1H, d, J=8.8 Hz), 7.35-7.65 (5H, m), 8.12 (1H, s).
The compounds shown in Table 42 were synthesized in a manner similar to that of Example 21-1 by using the corresponding starting materials.
The title compound was synthesized in a manner similar to that of Example 5-1 by using the corresponding reactant.
1H-NMR (CDCl3) δ ppm:
2.06 (3H, d, J=7.1 Hz), 3.84 (3H, s), 4.01 (3H, s), 5.83 (1H, q, J=7.1 Hz), 6.54 (1H, s), 6.68 (1H, dd, J=2.5, 8.9 Hz), 6.86 (1H, d, J=8.9 Hz), 6.95-7.05 (1H, m), 7.05-7.20 (1H, m), 7.30-7.55 (5H, m), 7.65 (1H, t, J=7.8 Hz), 7.90-8.05 (1H, m).
To a solution of methyl 6-(6-chloro-5-hydroxy-2-phenylindol-1-ylmethyl)pyridine-2-carboxylate (100 mg) in N,N-dimethylformamide (1 mL) were added, potassium carbonate (70.4 mg) and ethyl iodide (0.031 mL) and the mixture was stirred at room temperature for 22 hours. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic layer was washed successively with water and saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (83.9 mg).
1H-NMR (CDCl3) δ ppm:
1.50 (3H, t, J=7.0 Hz), 4.03 (3H, s), 4.15 (2H, q, J=7.0 Hz), 5.53 (2H, s), 6.55-6.75 (2H, m), 7.15-7.25 (2H, m), 7.30-7.50 (5H, m), 7.65-7.75 (1H, m), 7.95-8.05 (1H, m).
To a solution of methyl 6-(2-phenyl-5-triisopropylsilanyloxymethylindol-1-ylmethyl)pyridine-2-carboxylate (77.0 mg) in tetrahydrofuran (0.728 mL) was added tetrabutylammonium fluoride (1.0 mol/L tetrahydrofuran solution, 0.190 mL) under cooling with ice. The mixture was stirred under cooling with ice for one hour and then at room temperature for additional 2 hours. A saturated aqueous ammonium chloride solution was added to the reaction mixture and this resulting mixture was extracted with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting solvent: ethyl acetate-hexane) to obtain the title compound (44.4 mg).
1H-NMR (CDCl3) δ ppm:
1.58 (1H, t, J=5.9 Hz), 4.03 (3H, s), 4.78 (2H, d, J=5.9 Hz), 5.62 (2H, s), 6.65-6.75 (2H, m), 7.12 (1H, d, J=8.4 Hz), 7.18 (1H, dd, J=1.6, 8.4 Hz), 7.30-7.45 (5H, m), 7.60-7.75 (2H, m), 7.95-8.05 (1H, m).
The compounds shown in Tables 43 to 48 were synthesized in a manner similar to that of Example 9-1 by using the corresponding starting materials.
1H-NMR (CDCl3) δ ppm: 3.87 (3H, s), 4.03 (3H, s), 5.57 (2H, s), 6.60-6.70 (2H, m), 6.83 (1H, dd, J = 2.5, 8.9 Hz), 7.04 (1H, d, J = 8.9 Hz), 7.10-7.20 (1H, m), 7.20-7.40 (3H, m), 7.40-7.50 (1H, m), 7.67 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 2.33 (3H, s), 3.86 (3H, s), 4.02 (3H, s), 5.59 (2H, s), 6.60 (1H, s), 6.66 (1H, d, J = 7.8 Hz), 6.80 (1H, d, J = 2.5, 8.8 Hz), 7.01 (1H, d, J = 8.8 Hz), 7.10-7.30 (5H, m), 7.60-7.70 (1H, m), 7.98 (1H, d, J = 7.5 Hz).
1H-NMR (CDCl3) δ ppm: 3.87 (3H, s), 4.00 (3H, s), 5.42 (2H, s), 6.50-6.65 (2H, m), 6.83 (1H, dd, J = 2.5, 9.0 Hz), 7.05 (1H, d, J = 9.0 Hz), 7.17 (1H, d, J = 2.5 Hz), 7.20-7.40 (3H, m), 7.40-7.50 (1H, m), 7.60 (1H, t, J = 7.8 Hz), 7.85-8.00 (1H, m).
1H-NMR (CDCl3) δ ppm: 2.21 (3H, s), 3.87 (3H, s), 4.00 (3H, s), 5.35 (2H, s), 6.45-6.60 (2H, m), 6.80 (1H, dd, J = 2.5, 8.8 Hz), 7.03 (1H, d, J = 8.8 Hz), 7.10-7.35 (5H, m), 7.61 (1H, t, J = 7.8 Hz), 7.90-8.00 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.87 (3H, s), 4.03 (3H, s), 5.55 (2H, s), 6.65-6.75 (2H, m), 6.85 (1H, dd, J = 2.4, 8.9 Hz), 7.06 (1H, d, J = 8.9 Hz), 7.10-7.20 (1H, m), 7.25-7.40 (1H, m), 7.60-7.80 (2H, m), 7.95-8.05 (1H, m), 8.59 (1H, dd, J = 1.6, 4.9 Hz), 8.65-8.75 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.96 (3H, s), 4.03 (3H, s), 5.54 (2H, s), 6.55-6.75 (2H, m), 7.15-7.25 (2H, m), 7.30-7.45 (5H, m), 7.69 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.87 (3H, s), 4.04 (3H, s), 5.61 (2H, s), 6.65-6.85 (2H, m), 6.87 (1H, dd, J = 2.5, 9.0 Hz), 7.06 (1H, d, J = 9.0 Hz), 7.10-7.20 (1H, m), 7.25-7.40 (2H, m), 7.70 (1H, t, J = 7.8 Hz), 7.95-8.10 (1H, m), 8.55-8.65 (2H, m).
1H-NMR (CDCl3) δ ppm: 2.25 (3H, s), 3.89 (3H, s), 4.03 (3H, s), 5.56 (2H, s), 6.55-6.65 (1H, m), 6.65-6.75 (1H, m), 6.89 (1H, s), 7.08 (1H, s), 7.25-7.45 (5H, m), 7.66 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.95 (3H, s), 4.02 (3H, s), 5.43 (2H, s), 6.55-6.70 (2H, m), 7.05-7.25 (4H, m), 7.30-7.45 (2H, m), 7.65 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.95 (3H, s), 4.03 (3H, s), 5.53 (2H, s), 6.55-6.75 (2H, m), 7.00-7.25 (5H, m), 7.25-7.45 (1H, m), 7.70 (1H, t, J = 7.8 Hz), 7.95-8.10 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.95 (3H, s), 4.03 (3H, s), 5.49 (2H, s), 6.50-6.75 (2H, m), 7.00-7.15 (2H, m), 7.15-7.25 (2H, m), 7.30-7.45 (2H, m), 7.69 (1H, t, J = 7.8 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 2.29 (3H, s), 2.35 (3H, s), 4.03 (3H, s), 5.57 (2H, s), 6.50-6.75 (2H, m), 6.90 (1H, s), 7.25-7.50 (6H, m), 7.65 (1H, t, J = 7.8 Hz), 7.90-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.94 (3H, s), 4.02 (3H, s), 5.42 (2H, s), 6.55-6.70 (2H, m), 6.87 (1H, d, J = 11.3 Hz), 7.05-7.25 (3H, m), 7.30-7.45 (2H, m), 7.65 (1H, t, J = 7.8 Hz), 7.90-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.94 (3H, s), 4.03 (3H, s), 5.53 (2H, s), 6.55-6.75 (2H, m), 6.89 (1H, d, J = 11.3 Hz), 7.00-7.25 (4H, m), 7.25-7.45 (1H, m), 7.70 (1H, t, J = 7.8 Hz), 7.95-8.10 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.94 (3H, s), 4.03 (3H, s), 5.49 (2H, s), 6.50-6.75 (2H, m), 6.88 (1H, d, J = 11.3 Hz), 7.00-7.15 (2H, m), 7.19 (1H, d, J = 8.3 Hz), 7.30-7.45 (2H, m), 7.69 (1H, t, J = 7.8 Hz), 7.95-8.10 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.95 (3H, s), 4.03 (3H, s), 5.50 (2H, s), 6.60-6.80 (2H, s), 6.92 (1H, d, J = 11.3 Hz), 7.22 (1H, d, J = 8.0 Hz), 7.25-7.40 (1H, m), 7.65-7.80 (2H, m), 7.95-8.10 (1H, m), 8.55-8.65 (1H, m), 8.65-8.75 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.95 (3H, s), 4.05 (3H, s), 5.59 (2H, s), 6.60-6.80 (2H, m), 7.10-7.35 (4H, m), 7.37 (1H, dd, J = 3.0, 5.0 Hz), 7.71 (1H, t, J = 7.8 Hz), 8.00-8.10 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.95 (3H, s), 4.06 (3H, s), 5.58 (2H, s), 6.45-6.55 (1H, m), 6.55-6.75 (2H, m), 7.16 (1H, s), 7.20-7.25 (1H, m), 7.40-7.55 (2H, m), 7.69 (1H, t, J = 7.8 Hz), 8.00-8.10 (1H, m).
1H-NMR (CDCl3) δ ppm: 2.25 (3H, s), 3.89 (3H, s), 4.05 (3H, s), 5.61 (2H, s), 6.60-6.80 (2H, m), 6.90 (1H, br s), 7.06 (1H, s), 7.10-7.25 (2H, m), 7.30-7.40 (1H, m), 7.67 (1H, t, J = 7.8 Hz), 7.95-8.10 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 4.02 (3H, s), 5.47 (2H, s), 6.55-6.70 (2H, m), 6.80-6.90 (1H, m), 6.95-7.20 (5H, m), 7.64 (1H, t, J = 7.8 Hz), 7.97 (1H, d, J = 7.8 Hz).
1H-NMR (CDCl3) δ ppm: 3.96 (3H, s), 4.03 (3H, s), 5.50 (2H, s), 6.60-6.80 (2H, m), 7.20 (1H, s), 7.23 (1H, s), 7.25-7.40 (1H, m), 7.65-7.80 (2H, m), 8.02 (1H, d, J = 7.8 Hz), 8.55-8.65 (1H, m), 8.65-8.75 (1H, m).
1H-NMR (CDCl3) δ ppm: 0.84 (3H, t, J = 7.4 Hz), 1.23 (3H, d, J = 6.9 Hz), 1.45-1.80 (2H, m), 2.60-2.75 (1H, m), 3.93 (3H, s), 4.06 (3H, s), 5.50 (2H, s), 6.31 (1H, s), 6.40-6.50 (1H, m), 7.12 (1H, s), 7.15 (1H, s), 7.64 (1H, t, J = 7.9 Hz), 7.95-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 0.65-0.75 (2H, m), 0.85-1.00 (2H, m), 1.95-2.10 (1H, m), 4.02 (3H, s), 5.58 (2H, s), 6.55-6.65 (1H, m), 6.65-6.75 (1H, m), 6.91 (1H, dd, J = 1.9, 8.5 Hz), 7.01 (1H, d, J = 8.5 Hz), 7.30-7.45 (6H, m), 7.65 (1H, t, J = 7.9 Hz), 7.90-8.05 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.35 (3H, t, J = 7.1 Hz), 3.86 (3H, s), 4.32 (2H, q, J = 7.1 Hz), 5.23 (2H, s), 5.87 (1H, d, J = 3.5 Hz), 6.57 (1H, s), 6.88 (1H, dd, J = 2.4, 8.9 Hz), 6.98 (1H, d, J = 3.5 Hz), 7.10-7.30 (4H, m), 7.35-7.50 (2H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 3.86 (3H, s), 4.35 (2H, q, J = 7.1 Hz), 5.29 (2H, s), 5.95-6.05 (1H, m), 6.50-6.60 (1H, m), 6.89 (1H, dd, J = 2.5, 8.8 Hz), 7.00-7.15 (3H, m), 7.15-7.30 (3H, m), 7.35-7.45 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 3.87 (3H, s), 4.34 (2H, q, J = 7.1 Hz), 5.27 (2H, s), 6.00-6.05 (1H, m), 6.55-6.65 (1H, m), 6.91 (1H, dd, J = 2.4, 8.9 Hz), 7.04 (1H, d, J = 3.5 Hz), 7.10-7.15 (1H, m), 7.22 (1H, d, J = 8.9 Hz), 7.35-7.45 (1H, m), 7.75-7.85 (1H, m), 8.60-8.70 (1H, m), 8.70-8.80 (1H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 3.95 (3H, s), 4.35 (2H, q, J = 7.1 Hz), 5.25 (2H, s), 5.95-6.05 (1H, m), 6.50-6.60 (1H, m), 7.06 (1H, d, J = 3.5 Hz), 7.15 (1H, s), 7.30-7.55 (6H, m).
1H-NMR (CDCl3) δ ppm: 1.36 (3H, t, J = 7.2 Hz), 3.94 (3H, s), 4.33 (2H, q, J = 7.2 Hz), 5.17 (2H, s), 5.90 (1H, d, J = 3.5 Hz), 6.56 (1H, s), 6.95-7.35 (5H, m), 7.35-7.50 (2H, m).
1H-NMR (CDCl3) δ ppm: 1.37 (3H, t, J = 7.1 Hz), 2.32 (3H, s), 3.88 (3H, s), 4.35 (2H, q, J = 7.1 Hz), 5.27 (2H, s), 5.90-6.05 (1H, m), 6.50-6.55 (1H, m), 7.00-7.10 (3H, m), 7.30-7.55 (5H, m).
1H-NMR (CDCl3) δ ppm: 1.38 (3H, t, J = 7.2 Hz), 3.94 (3H, s), 4.36 (2H, q, J = 7.2 Hz), 5.30 (2H, s), 6.00-6.10 (1H, m), 6.50-6.65 (2H, m), 7.07 (1H, d, J = 3.3 Hz), 7.12 (1H, s), 7.30-7.35 (1H, m), 7.45-7.65 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.86 (3H, s), 3.89 (3H, s), 5.35 (2H, s), 6.55-6.65 (1H, m), 6.83 (1H, dd, J = 2.5, 8.8 Hz), 7.00-7.15 (3H, m), 7.20-7.40 (4H, m), 7.40-7.45 (1H, m), 7.75-7.85 (1H, m), 7.85-7.95 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.80-3.90 (6H, m), 5.21 (2H, s), 6.50-6.60 (1H, m), 6.83 (1H, dd, J = 2.4, 8.9 Hz), 6.95-7.05 (1H, m), 7.05-7.40 (6H, m), 7.45-7.55 (1H, m), 7.60-7.70 (1H, m), 7.80-7.90 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.69 (3H, s), 3.86 (3H, s), 3.89 (3H, s), 5.38 (2H, s), 6.55-6.65 (1H, m), 6.81 (1H, dd, J = 2.4, 8.9 Hz), 6.85-7.20 (6H, m), 7.20-7.40 (2H, m), 7.75-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.80-3.95 (6H, m), 5.36 (2H, s), 6.60-6.70 (1H, m), 6.85 (1H, dd, J = 2.5, 8.8 Hz), 7.00-7.20 (3H, m), 7.25-7.40 (2H, m), 7.60-7.75 (1H, m), 7.75-7.85 (1H, m), 7.85-7.95 (1H, m), 8.55-8.65 (1H, m), 8.65-8.75 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.80-3.90 (6H, m), 5.27 (2H, s), 6.60 (1H, s), 6.81 (1H, dd, J = 2.5, 8.8 Hz), 6.95-7.10 (2H, m), 7.10-7.30 (4H, m), 7.30-7.45 (2H, m), 7.70-7.90 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.80-3.95 (6H, m), 5.37 (2H, s), 6.55-6.65 (1H, m), 6.82 (1H, dd, J = 2.5, 8.8 Hz), 7.00-7.25 (6H, m), 7.25-7.40 (2H, m), 7.75-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 3.80-3.95 (6H, m), 5.32 (2H, s), 6.55 (1H, s), 6.81 (1H, dd, J = 2.5, 8.8 Hz), 7.00-7.15 (5H, m), 7.25-7.40 (3H, m), 7.75-7.95 (2H, m).
1H-NMR (CDCl3) δ ppm: 2.16 (3H, s), 3.86 (6H, s), 5.13 (2H, s), 6.40-6.50 (1H, m), 6.81 (1H, dd, J = 2.5, 8.8 Hz), 6.90-7.05 (1H, m), 7.05-7.40 (7H, m), 7.60-7.70 (1H, m), 7.75-7.90 (1H, m).
1H-NMR (CDCl3) δ ppm: 3.85 (3H, s), 3.92 (3H, s), 5.39 (2H, s), 6.55 (1H, s), 6.87 (1H, dd, J = 2.5, 8.8 Hz), 7.10 (1H, d, J = 2.5 Hz), 7.30 (1H, d, J = 8.8 Hz), 7.35-7.65 (5H, m), 8.12 (1H, s).
1H-NMR (CDCl3) δ ppm: 3.85-4.00 (6H, m), 5.34 (2H, s), 6.50-6.60 (1H, m), 7.10-7.20 (2H, m), 7.35-7.65 (5H, m), 8.14 (1H, s).
1H-NMR (CDCl3) δ ppm: 2.31 (3H, s), 3.87 (3H, s), 3.92 (3H, s), 5.37 (2H, s), 6.53 (1H, s), 7.03 (1H, s), 7.15 (1H, s), 7.35-7.65 (5H, m), 8.11 (1H, s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.51 (2H, s), 6.67 (1H, s), 6.78 (1H, dd, J = 2.5, 8.8 Hz), 6.80-6.90 (1H, m), 7.13 (1H, d, J = 2.5 Hz), 7.28 (1H, d, J = 8.8 Hz), 7.40-7.60 (3H, m), 7.65-7.75 (1H, m), 7.80-7.95 (2H, m), 13.00-13.50 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.51 (2H, s), 6.68 (1H, s), 6.79 (1H, dd, J = 2.4, 8.9 Hz), 7.05-7.15 (2H, m), 7.25-7.55 (7H, m), 7.70-7.80 (1H, m), 12.95 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 2.31 (3H, s), 3.77 (3H, s), 5.51 (2H, s), 6.50-6.80 (3H, m), 7.05-7.45 (6H, m), 7.75-7.95 (2H, m), 12.85-13.65 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.78 (3H, s), 5.34 (2H, s), 6.56 (1H, s), 6.60-6.70 (1H, m), 6.79 (1H, dd, J = 2.3, 8.9 Hz), 7.15 (1H, d, J = 2.3 Hz), 7.25-7.65 (5H, m), 7.70-7.90 (2H, m), 12.85-13.50 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.29 (2H, s), 6.50-6.55 (1H, m), 6.79 (1H, dd, J = 2.4, 8.9 Hz), 6.95-7.05 (1H, m), 7.10-7.15 (1H, m), 7.25-7.55 (6H, m), 7.55-7.65 (1H, m), 7.65-7.75 (1H, m), 12.60-13.20 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.32 (2H, s), 6.06 (1H, d, J = 3.5 Hz), 6.55 (1H, s), 6.84 (1H, dd, J = 2.5, 8.8 Hz), 7.01 (1H, d, J = 3.5 Hz), 7.05-7.15 (1H, m), 7.25-7.60 (5H, m), 12.99 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 2.17 (3H, s), 3.77 (3H, s), 5.28 (2H, s), 6.47 (1H, s), 6.55-6.65 (1H, m), 6.75 (1H, dd, J = 2.5, 8.8 Hz), 7.13 (1H, d, J = 2.5 Hz), 7.15-7.40 (5H, m), 7.70-7.90 (2H, m), 13.00-13.35 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.53 (2H, s), 6.72 (1H, s), 6.79 (1H, dd, J = 2.5, 8.9 Hz), 6.85-6.95 (1H, m), 7.15 (1H, d, J = 2.5 Hz), 7.32 (1H, d, J = 8.9 Hz), 7.40-7.50 (1H, m), 7.80-7.95 (2H, m), 8.00-8.15 (1H, m), 8.58 (1H, dd, J = 1.5, 4.8 Hz), 8.75-8.85 (1H, m), 13.24 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.87 (3H, s), 5.55 (2H, s), 6.60-6.80 (2H, m), 7.32 (1H, s), 7.35-7.50 (3H, m), 7.50-7.60 (3H, m), 7.75-7.95 (2H, m), 12.80-13.70 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.69 (3H, s), 3.77 (3H, s), 5.50 (2H, s), 6.55-6.65 (1H, m), 6.76 (1H, dd, J = 2.5, 8.8), 6.90-7.20 (5H, m), 7.26 (1H, d, J = 8.8 Hz), 7.30-7.45 (2H, m), 7.45-7.55 (1H, m), 7.70-7.80 (1H, m), 12.96 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.44 (2H, s), 6.23 (1H, d, J = 3.5 Hz), 6.61 (1H, s), 6.83 (1H, dd, J = 2.5, 8.8 Hz), 7.00-7.15 (2H, m), 7.20-7.35 (1H, m), 7.35-7.60 (4H, m), 13.04 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.76 (3H, s), 5.50 (2H, s), 6.57 (1H, s), 6.77 (1H, dd, J = 2.5, 8.8 Hz), 7.11 (1H, d, J = 2.5 Hz), 7.33 (1H, d, J = 8.8 Hz), 7.35-7.55 (4H, m), 7.55-7.70 (2H, m), 13.60-14.50 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.78 (3H, s), 5.57 (2H, s), 6.75-6.90 (2H, m), 7.00-7.20 (2H, m), 7.30-7.50 (3H, m), 7.70-7.85 (2H, m), 8.15-8.30 (1H, m), 8.65-8.90 (2H, m).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.61 (2H, s), 6.75-6.90 (3H, m), 7.16 (1H, d, J = 2.5 Hz), 7.33 (1H, d, J = 9.0 Hz), 7.60-7.70 (2H, m), 7.80-7.95 (2H, m), 8.55-8.65 (2H, m), 13.25 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.44 (2H, s), 6.24 (1H, d, J = 3.4 Hz), 6.65 (1H, s), 6.84 (1H, dd, J = 2.3, 8.9 Hz), 7.06 (1H, d, J = 3.4 Hz), 7.11 (1H, d, J = 2.3 Hz), 7.45-7.60 (2H, m), 7.95-8.05 (1H, m), 8.60-8.70 (1H, m), 8.75-8.85 (1H, m), 13.03 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 2.18 (3H, s), 3.81 (3H, s), 5.51 (2H, s), 6.55-6.70 (2H, m), 7.10 (1H, s), 7.15 (1H, s), 7.30-7.60 (5H, m), 7.75-7.90 (2H, m), 12.95-13.50 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.36 (2H, s), 6.59 (1H, s), 6.78 (1H, dd, J = 2.3, 8.9 Hz), 7.00-7.10 (1H, m), 7.13 (1H, d, J = 2.3 Hz), 7.20-7.55 (7H, m), 7.65-7.80 (1H, m), 12.91 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.52 (2H, s), 6.67 (1H, s), 6.79 (1H, dd, J = 2.4, 8.9 Hz), 7.05-7.15 (2H, m), 7.15-7.55 (7H, m), 7.70-7.80 (1H, m), 12.94 (1H, s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.48 (2H, s), 6.55-6.65 (1H, m), 6.77 (1H, dd, J = 2.5, 8.8 Hz), 7.00-7.15 (2H, m), 7.20-7.60 (7H, m), 7.70-7.80 (1H, m), 12.94 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 2.11 (3H, s), 3.77 (3H, s), 5.24 (2H, s), 6.40-6.50 (1H, m), 6.76 (1H, dd, J = 2.5, 8.8 Hz), 6.95-7.15 (2H, m), 7.20-7.40 (7H, m), 7.65-7.80 (1H, m), 12.90 (1H, s).
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.52 (2H, s), 6.50-6.60 (1H, m), 6.83 (1H, dd, J = 2.5, 8.8 Hz), 7.05-7.15 (1H, m), 7.30-7.65 (6H, m), 8.66 (1H, s), 12.50-13.55 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.87 (3H, s), 5.44 (2H, s), 6.60-6.75 (2H, m), 7.20-7.70 (6H, m), 7.75-7.90 (2H, m), 12.50-14.00 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.86 (3H, s), 5.55 (2H, s), 6.65-6.75 (1H, m), 6.80-6.95 (1H, m), 7.15-7.30 (1H, m), 7.32 (1H, s), 7.35-7.65 (4H, m), 7.80-7.95 (2H, m), 12.50-14.00 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.86 (3H, s), 5.52 (2H, s), 6.60-6.70 (1H, m), 6.75-6.90 (1H, m), 7.20-7.40 (3H, m), 7.50-7.70 (3H, m), 7.80-7.95 (2H, m), 13.20 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 1.93 (3H, d, J = 7.0 Hz), 3.73 (3H, s), 5.72 (1H, q, J = 7.0 Hz), 6.45-6.70 (2H, m), 6.90 (1H, d, J = 9.0 Hz), 7.05-7.20 (2H, m), 7.35-7.70 (5H, m), 7.80-8.00 (2H, m). ESI-MS (m/z): 373 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 3.86 (3H, s), 5.44 (2H, s), 6.16 (1H, d, J = 3.3 Hz), 6.55-6.65 (1H, m), 7.05 (1H, d, J = 3.3 Hz), 7.28 (1H, s), 7.40-7.65 (5H, m), 7.72 (1H, s), 12.50-13.50 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 2.25 (3H, s), 2.28 (3H, s), 5.51 (2H, s), 6.55-6.70 (2H, m), 7.13 (1H, s), 7.30-7.60 (6H, m), 7.75-7.95 (2H, m), 12.50-14.00 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.86 (3H, s), 5.41 (2H, s), 6.50-6.70 (2H, m), 7.15-7.60 (6H, m), 7.70-7.90 (2H, m). ESI-MS (m/z): 395 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 3.85 (3H, s), 5.53 (2H, s), 6.65-6.75 (1H, m), 6.75-6.90 (1H, m), 7.15-7.60 (6H, m), 7.75-7.95 (2H, m). ESI-MS (m/z): 395 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 3.85 (3H, s), 5.32 (2H, s), 6.07 (1H, d, J = 3.5 Hz), 6.58 (1H, s), 7.01 (1H, d, J = 3.5 Hz), 7.25-7.45 (3H, m), 7.45-7.65 (3H, m), 13.02 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 3.85 (3H, s), 5.49 (2H, s), 6.55-6.80 (2H, m), 7.20-7.45 (4H, m), 7.55-7.70 (2H, m), 7.70-7.95 (2H, m). ESI-MS (m/z): 395 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 3.85 (3H, s), 5.52 (2H, s), 6.58 (1H, s), 7.30 (1H, d, J = 8.5 Hz), 7.35-7.60 (6H, m), 8.62 (1H, s). ESI-MS (m/z): 367 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 3.86 (3H, s), 5.55 (2H, s), 6.82 (1H, s), 7.05-7.20 (1H, m), 7.35 (1H, d, J = 8.5 Hz), 7.49 (1H, d, J = 12.0 Hz), 7.65-8.00 (3H, m), 8.40-8.55 (1H, m), 8.65-8.80 (1H, m), 9.00-9.15 (1H, m). ESI-MS (m/z): 378 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 3.86 (3H, s), 5.60 (2H, s), 6.42 (1H, d, J = 7.8 Hz), 6.70-6.80 (1H, m), 7.25-7.50 (3H, m), 7.60-7.90 (4H, m). ESI-MS (m/z): 399, 401 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 1.38 (3H, t, J = 6.9 Hz), 4.11 (2H, q, J = 6.9 Hz), 5.52 (2H, s), 6.45-6.60 (1H, m), 6.60-6.70 (1H, m), 7.32 (1H, s), 7.35-7.60 (6H, m), 7.65-7.85 (2H, m).
1H-NMR (DMSO-d6) δ ppm: 2.24 (3H, s), 3.80 (3H, s), 5.38 (2H, s), 6.13 (1H, d, J = 3.5 Hz), 6.45-6.55 (1H, m), 7.00-7.15 (2H, m), 7.33 (1H, s), 7.35-7.65 (5H, m), 12.50-13.50 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 3.86 (3H, s), 5.63 (2H, s), 6.65-6.90 (3H, m), 7.27 (1H, s), 7.60-8.10 (5H, m). ESI-MS (m/z): 383, 385 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 3.84 (3H, s), 5.53 (2H, s), 6.32 (1H, d, J = 3.5 Hz), 6.60-6.70 (1H, m), 6.85-6.95 (1H, m), 7.09 (1H, d, J = 3.5 Hz), 7.24 (1H, s), 7.70-7.90 (2H, m), 8.05-8.15 (1H, m), 13.07 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 2.19 (3H, s), 3.81 (3H, s), 5.56 (2H, s), 6.55-6.75 (2H, m), 7.07 (1H, s), 7.15 (1H, s), 7.25-7.40 (1H, m), 7.55-7.90 (4H, m). ESI-MS (m/z): 379 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 2.24 (3H, s), 3.80 (3H, s), 5.45 (2H, s), 6.52 (1H, s), 7.07 (1H, s), 7.25 (1H, s), 7.35-7.70 (5H, m), 8.42 (1H, s). ESI-MS (m/z): 363 (M + H)+
1H-NMR (DMSO-d6) δ ppm: 3.77 (3H, s), 5.43 (2H, s), 6.66 (1H, s), 6.75-6.90 (2H, m), 7.14 (1H, d, J = 2.5 Hz), 7.25-7.70 (4H, m), 7.75-7.95 (2H, m). ESI-MS (m/z): 395 (M + M)+
1H-NMR (DMSO-d6) δ ppm: 3.87 (3H, s), 5.55 (2H, s), 6.75 (1H, s), 6.85-7.00 (1H, m), 7.33 (1H, s), 7.35-7.55 (1H, m), 7.63 (1H, s), 7.80-7.95 (2H, m), 8.00-8.15 (1H, m), 8.50-8.65 (1H, m), 8.70-8.85 (1H, m), 12.80-13.60 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 4.55 (2H, d, J = 4.7 Hz), 4.95-5.15 (1H, m), 5.56 (2H, s), 6.65-6.75 (2H, m), 7.05-7.15 (1H, m), 7.28 (1H, d, J = 8.2 Hz), 7.35-7.50 (3H, m), 7.50-7.65 (3H, m), 7.75-7.95 (2H, m), 13.23 (1H, br s).
1H-NMR (DMSO-d6) δ ppm: 0.77 (3H, t, J = 7.4 Hz), 1.17 (3H, d, J = 6.9 Hz), 1.40-1.70 (2H, m), 2.80-2.95 (1H, m), 3.83 (3H, s), 5.45-5.65 (2H, m), 6.30 (1H, s), 6.70 (1H, d, J = 7.3 Hz), 7.20 (1H, s), 7.55 (1H, s), 7.80-7.95 (2H, m), 13.00-13.50 (1H, br).
1H-NMR (DMSO-d6) δ ppm: 0.55-0.70 (2H, m), 0.85-0.95 (2H, m), 1.90-2.05 (1H, m), 5.52 (2H, s), 6.60 (1H, s), 6.72 (1H, d, J = 7.6 Hz), 6.86 (1H, dd, J = 1.6, 8.5 Hz), 7.20 (1H, d, J = 8.5 Hz), 7.25-7.65 (6H, m), 7.75-7.90 (2H, m), 12.00-14.50 (1H, br).
Using Rat Kidney BD Marathon-Ready cDNA (Nippon Becton Dickinson Company, Ltd.) as a template, a forward primer shown in SEQ ID NO. 1, and a reverse primer shown in SEQ ID NO. 2, a first run of PCR was carried out using KOD-Plus-Ver 2.0 (Toyobo Co., Ltd.). Further, using this amplification product as a template, a forward primer shown in SEQ ID NO. 3, and a reverse primer shown in SEQ ID NO. 4, a second run of PCR was carried out in the same manner. The amplification product obtained by the second run of PCR was incorporated into a vector (pcDNA3.1 D/V5-His-TOPO (registered trademark), Invitrogen Japan K. K.). By a conventional method, the vector containing this amplification product was introduced to E. coli (One Shot TOP10 Competent Cells, Invitrogen Corporation) to transform. This transformed E. coli was cultured in an LB agar medium for one day. After the culture, colonies were selected and cultured in an LB liquid medium containing 50 μg/mL of ampicillin. After the culture, the vector was purified using a QIAprep Spin Miniprep Kit (Qiagen K. K.). The base sequence of the insertion site of this vector (SEQ ID NO. 5) was compared with the rat EP1 base sequence (Ptger1) registered as an accession number NM—013100 in well-known database (NCBI), and as a result, they all matched except for a single base. Further, the amino acid sequence translated by the base sequence completely matched the amino acid sequence of the rat EP1 receptor registered as an NCBI accession number NP—037232. Therefore, it was confirmed that the cloned gene sequence was a base sequence of the rat EP1 receptor and the obtained amino acid sequence was that of the rat EP1 receptor. The pcDNA3.1 D/V5-His-TOPO (registered trademark) to which the nucleic acid shown in SEQ ID NO. 5 had been inserted was taken as a rat EP1-expressing vector.
COS-1 cells (Dainippon Sumitomo Pharma Co., Ltd.) were cultured until it reached confluence in an incubator at 37° C. under a 5% CO2 gas condition, using a D-MEM liquid medium (high glucose and L-glutamine contained, Invitrogen Corporation) to which a penicillin-streptomycin solution (Invitrogen Corporation, final concentration: 100 U/mL as benzylpenicillin; 100 μg/mL as streptomycin) as an antibiotic, MEM nonessential amino acids (Invitrogen Corporation, final Concentration: 0.1 mM), and fetal calf serum (Sanko Junyaku Co., Ltd., final concentration: 10%) were added.
The cells that had reached confluence were stripped with 0.05% trypsin/0.53 mM EDTA 4Na (Invitrogen Japan K. K.) and resuspended in the liquid medium. The resuspended cells were diluted and cultured in the liquid medium at a spread ratio from 1:4 to 1:8.
The cells that had reached confluence were stripped with 0.05% trypsin/0.53 mM EDTA•4Na, and resuspended in a D-MEM liquid medium (high glucose and L-glutamine contained, Invitrogen Corporation) to which an MEM nonessential amino acid (final concentration: 0.1 mM) and fetal calf serum (final concentration: 10%) were added. In each well of a Poly D-lysine-coated 96-well microplate (BD BioCoat (registered trademark), Nippon Becton Dickinson Company, Ltd.), this resuspended cell suspension culture was prepared to be 5×104 cells/well in 100 μL of the liquid medium, and seeded thereon. After seeding, the cells were cultured in an incubator at 37° C. under a 5% CO2 gas condition. At a point when the cells for introduction of a rat EP1-expressing vector were adhered (about 2 hours after seeding), introduction of the rat EP1-expressing vector was carried out in the following order.
For introduction of the rat EP1-expressing vector, Lipofectamine 2000 (Invitrogen Japan K. K.) was used. The rat EP1-expressing vector was diluted with OPTI-MEM (registered trademark) I Reduced-Serum Medium (Invitrogen Japan K. K.) to 200 ng/25 μL/well, and at the same time, Lipofectamine 2000 (Invitrogen Japan K. K.n) was also diluted with OPTI-MEM (registered trademark) I Reduced-Serum Medium (Invitrogen Japan K. K.) to 0.5 μL/25 μL/well, followed by incubation at room temperature for 5 minutes. After the incubation for 5 minutes, in order to form a complex of the rat EP1-expressing vector/Lipofectamine 2000, the diluted rat EP1-expressing vector and the diluted Lipofectamine 2000 were mixed and incubated at room temperature for 30 minutes. After the incubation for 30 minutes, the complex of the rat EP1-expressing vector/Lipofectamine 2000 was distributed to the cells for introduction of the rat EP1-expressing vector at 50 μL/well. The cells to which the complex of the rat EP1-expressing vector/Lipofectamine 2000 had been distributed were cultured in an incubator at 37° C. for 20 hours under a 5% CO2 gas condition. After the culture for 20 hours, the cells were used for measurement of an intracellular calcium concentration as rat EP1 receptor-expressing cells.
Using the rat EP1 receptor-expressing cells, the inhibitory effect of each test compound on the increased intracellular calcium concentration induced by prostaglandin E2 was studied in Method A or Method B as shown below.
A 10 mM solution of each test compound in dimethyl sulfoxide was diluted with an assay buffer (20 mM HEPES/Hank's Balanced Salt Solution (HBSS), pH 7.2).
The rat EP1 receptor-expressing cells were washed with the assay buffer. 100 μL of a fluorescent calcium indicator (Fluo-4 NW Calcium Assay Kit (Molecular Probes): prepared by the protocol of the same product, Invitrogen Corporation, 2.5 mM probenecid contained) was added to each well, followed by incubation in an incubator at 37° C. for 60 minutes. Then, all the cell supernatants were aspirated and washed with the assay buffer. After the washing, 100 μL of an assay buffer containing 2.5 mM probenecid was added to each well, and the intracellular calcium concentration was measured immediately.
The intracellular calcium concentration was measured as a fluorescent signal using FlexStation (registered trademark) (manufactured by Molecular Devices). 50 μL of each test compound that had been diluted with the assay buffer (final concentrations: 1 nM to 10 μM) was added to each well after 20 seconds from initiating the reading of the fluorescent signal, and the fluorescence signal was measured for 60 seconds. Then, 50 μL of a prostaglandin E2 buffer solution was added to each well (final concentration 10 nM) and the fluorescence signal was measured for 60 seconds.
A 10 mM solution of each test compound in dimethyl sulfoxide was diluted in an assay buffer (20 mM HEPES/Hank's Balanced Salt Solution (HBSS), pH 7.2).
The rat EP1 receptor-expressing cells were washed with the assay buffer. 100 μL of a fluorescent calcium indicator (Calcium kit II, Fluo 4 (Dojindo Laboratories): prepared by the protocol of the same product, Invitrogen Japan K. K., 2.5 mM probenecid contained) was added to each well, followed by incubation in an incubator at 37° C. for 60 minutes. Then, the intracellular calcium concentration was measured immediately.
The intracellular calcium concentration was measured as a fluorescent signal using FDSS (registered trademark) 7000 (manufactured by Hamamatsu Photonics K. K.). 50 μL of each test compound (final concentrations: 1 nM to 10 μM) was added to each well after 20 seconds from initiating the reading of the fluorescent signal, and the fluorescence signal was measured for 60 seconds. Then, 50 μL of a prostaglandin E2 buffer solution was added to each well (final concentration 10 nM) and the fluorescence signal was measured for 60 seconds.
In Method A or Method B, as a fluorescent signal obtained by the addition of the prostaglandin E2 with the addition of the assay buffer instead of the test compound was taken as 100% and a signal obtained without the addition of any of the test compound and the prostaglandin E2 was taken as 0%, the concentration of the test compound showing 50% inhibition from the concentration-response curve was taken as an IC50 value. As the values of the EP1 receptor antagonism, the obtained IC50 values of each test compound were shown in Table 49 below. As Comparative Example 1,3-(5-methyl-2-phenylindol-1-yl)carboxylic acid (Compound 27) described in Patent literature 1 was tested in the similar way. Further, as Comparative Example 2, sodium 6-(6-chloro-3-isobutylindol-1-yl)pyridine-2-carboxylate (Compound 12g) described in Non-Patent literature 5 was tested in the similar way. The results of Comparative Example 1 and Comparative Example 2 were shown in Table 49.
As shown in Table 49, whereas Comparative Example 1 do not exhibit EP1 receptor antagonism, it is apparent that the compounds of the present invention exhibit potent EP1 receptor antagonism. Further, it is apparent that the compounds of the present invention exhibit potent EP1 receptor antagonism, as also compared with Comparative Example 2.
Female SD rats were used. Under urethane anesthesia (1.25 g/kg, administered subcutaneously), a tracheal cannula (Size 8, HIBIKI) and a femoral vein cannula for administration (23 G needle-equipped PE50) were inserted thereinto. The bladder cannula (PE50) was inserted from the bladder apex. The bladder cannula was connected to a three-way stopcock, and then, one was connected to a pressure transducer and the other was connected to a syringe filled with saline. Saline was injected to the bladder at an injection rate of 3.6 mL/hour and the bladder contraction pressure was recorded at the time of injection with a recorder (RECTI-HORITZ-8K, NEC Corporation). After 10 minutes from stabilization of the bladder contraction pressure during urination, sulprostone was administered subcutaneously (0.3 mg/kg). Then, at the time point when the bladder contraction pressure became constant, a test agent was administered intravenously (1.0 mg/kg). An average bladder contraction pressure during the 10 minutes period before administration of sulprostone was taken as a baseline (0%). Further, an average bladder contraction pressure during the 10 minutes period before administration of the test agent was taken as a maximum bladder contraction pressure (100%). The average bladder contraction pressures were measured during 5 minutes before and after at 15 minutes and 60 minutes from administration of the test agent. The ratio of this measured value to the maximum bladder contraction pressure was calculated by the following equation and taken as an average bladder contraction rate after administration of the test agent: (Average Bladder Contraction Rate after Administration of Test Agent (%))=(Average Bladder Contraction Pressure after Administration of Test Agent)/(Maximum Bladder Contraction Pressure). In addition, the difference between the maximum bladder contraction rate (100%) and the average bladder contraction rate (%) after administration of the test agent was calculated by the following equation and taken as a bladder contraction inhibition rate of the test agent: (Bladder Contraction Inhibition Rate)=100%−(Average Bladder Contraction Rate after Administration of Test Agent (%)). The results were shown in Table 50.
From the results above, it was found that the compounds of the present invention had potent and sustained inhibition of the bladder contraction even when administered in vivo.
SEQ ID NO. 1 is a sequence of a forward primer (5′ primer) used for amplification of DNA of SEQ ID NO. 5.
SEQ ID NO. 2 is a sequence of a reverse primer (3′ primer) used for amplification of DNA of SEQ ID NO. 5.
SEQ ID NO. 3 is a sequence of a forward primer (5′ primer) used for amplification of DNA of SEQ ID NO. 5.
SEQ ID NO. 4 is a sequence of a reverse primer (3′ primer) used for amplification of DNA of SEQ ID NO. 5.
SEQ ID NO. 5 is a DNA sequence for expressing a rat EP1 receptor which is amplified using the primers of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, and SEQ ID NO. 4.
Number | Date | Country | Kind |
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2009-173862 | Jul 2009 | JP | national |
2009-252862 | Nov 2009 | JP | national |
Filing Document | Filing Date | Country | Kind | 371c Date |
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PCT/JP2010/062533 | 7/26/2010 | WO | 00 | 1/26/2012 |