Indolizine derivatives and process for preparing the same

This invention relates to heterocyclic compounds and is concerned with novel indolizine derivatives and with a method of preparing the said novel derivatives.
The indolizine derivatives with which the present invention is concerned are the compounds represented by the general formula: ##STR3## and the pharmaceutically acceptable acid addition salts thereof, for example the oxalate or hydrochloride, wherein:
R represents a branched- or straight-chain alkyl radical having from 1 to 8 carbon atoms, or a phenyl group non-substituted or bearing one or two substituents, which may be the same or different, selected from halogen atoms, for example fluorine, chlorine and bromine and from lower alkyl and alkoxy groups for example methyl and methoxy,
X.sub.1 represents hydrogen, chlorine, bromine, iodine, methyl or methoxy,
A represents a group selected from: ##STR4## in which X.sub.2 represents hydrogen, chlorine, bromine, iodine, methyl or methoxy and X.sub.3 represents hydrogen, chlorine, bromine, iodine or methyl,
R.sub.1 represents a methyl, ethyl, n-propyl or n-butyl radical,
n represents an integer in the range of 2 to 6 inclusive, with the proviso that when both X.sub.2 and X.sub.3 represent hydrogen or methyl, X.sub.1 is other than hydrogen.
In the aforesaid general formula I, R represents, preferably, a branched- or straight-chain alkyl radical having from 1 to 8 carbon atoms, a phenyl radical, a mono-fluoro-, mono-chloro-, mono-bromo-, mono-methyl- or mono-methoxy-phenyl radical, a di-fluoro-, di-chloro-, di-bromo-phenyl radical or a methyl-phenyl radical substituted in the aromatic moiety by an atom of fluorine, chlorine or bromine. The indolizine derivatives of the invention have been found to possess useful pharmacological properties capable of rendering them of considerable value in the treatment of certain pathological syndromes of the heart, more particularly in the treatment of angina pectoris and auricular and ventricular cardiac arrhythmias of various origins.
The present invention is also concerned with pharmaceutical and veterinary compositions containing, as active principle, at least one indolizine derivative of formula I or a pharmaceutically acceptable acid addition salt thereof, in association with a pharmaceutical carrier or excipient therefor.
Another object of the present invention is to provide a process for preparing pharmaceutical or veterinary compositions whereby at least one indolizine derivative of formula I or a pharmaceutically acceptable acid addition salt thereof, is associated with a pharmaceutical carrier or excipient therefor.
Yet another object of the present invention is to provide a method of treating pathological syndromes of the heart and particularly angina pectoris and cardiac arrhythmias in a subject needing such treatment which method comprises administering to said subject an effective dose of at least one indolizine derivative of formula I or a pharmaceutically acceptable acid addition salt thereof.
Daily dosages will be preferably from 100 to 300 mg of active principle by oral route and preferably from 1 to 3 mg of active principle by parenteral route to a subject weighing 60 kgs.
The compounds of formula I can be prepared, in accordance with the invention, by condensing, in an inert solvent such as, for example benzene or toluene, a bromoalkoxy-benzoyl-indolizine of the general formula: ##STR5## wherein X.sub.1, R, A and n have the same meaning as in formula I, with a secondary amine of the general formula: ##STR6## in which R.sub.1 has the same meaning as in Formula I, to form the required indolizine derivative of formula I which, if desired, is reacted with an appropriate organic or inorganic acid to provide a pharmaceutically acceptable acid addition salt thereof.
The compounds of formula I may alternatively be prepared by condensing, advantageously in an aprotic solvent such as, for example, acetone, methyl ethyl ketone or toluene, an alkali metal salt, preferably the potassium or sodium salt of an appropriately substituted indolizine derivative represented by the general formula: ##STR7## in which R, A and X.sub.1 have the same meaning as in formula I, with an alkylamino derivative of the general formula: ##STR8## or an acid addition salt thereof, in which Z represents a halogen atom such as chlorine or bromine or a p-toluenesulphonyloxy group and n and R.sub.1 have the same meaning as in formula I to give the required indolizine derivative which, if desired, is reacted with an appropriate organic or inorganic acid to provide a pharmaceutically acceptable acid addition salt thereof.
In accordance with a further aspect of the invention, the indolizine derivatives of formula I in which X.sub.1 represents chlorine, bromine or iodine and A represents the group R.sub.3 or a group R.sub.2 in which X.sub.2 represents chlorine, bromine, iodine, methyl or methoxy and X.sub.3 represents chlorine, bromine, iodine or methyl can also be prepared by reacting an indolizine derivative of general formula: ##STR9## in which R, R.sub.1 and n have the same meaning as in formula I and A represents the group R.sub.3 or a group R.sub.2 in which X.sub.2 represents chlorine, bromine, iodine, methyl or methoxy and X.sub.3 represents chlorine, bromine, iodine or methyl:
(a) either with N-chlorosuccinimide, the reaction taking place in a suitable medium such as dichlorethane and between 0.degree. C. and room-temperature, to obtain the required compound of formula I in which X.sub.1 represents chlorine, in free base form,
(b) or with bromine or iodine, the reaction taking place at room-temperature in a suitable solvent such as dioxan and in the presence of an alkali metal acetate, for instance sodium acetate, to obtain the required compound of formula I in which X.sub.1 represents bromine or iodine, in free base form,
the free base so obtained being then reacted, if desired, with an organic or inorganic acid, to provide a pharmaceutically acceptable acid addition salt thereof.
The compounds of formula II can be obtained by condensing, advantageously in an inert medium such as for example acetone or methyl ethyl ketone, an alkali metal salt, preferably the potassium or sodium salt, of a compound of formula IV hereabove, with a dibromoalkane of the general formula:
Br--(CH.sub.2).sub.n --Br VII
in which n has the same meaning as in formula I to obtain the required compound of formula II.
The compounds of formula IV in which A represents the group R.sub.2 are either compounds described in copending British patent application No. 2,051,812 A published Jan. 21, 1981 or compounds which can be prepared in accordance with methods described in the aforesaid British patent application.
The other compounds of formula IV, i.e., those in which A represents the group R.sub.3 can be obtained by reacting the appropriate 2-substituted-indolizine with 2,3-dichloro-4-acetyloxy or 4-tosyloxy-benzoyl chloride. This benzoyl chloride derivative is itself prepared by acetylating 1,2-dichloro-anisole in accordance with the conditions of the FRIEDEL and CRAFTS reaction oxydating the acetyl derivative so obtained with sodium hypochlorite to form the corresponding benzoic acid derivative, demethylating with hydriodic acid in acetic acid to obtain 2,3-dichloro-4-hydroxybenzoic acid. This last-cited compound is then reacted with acetyl chloride or tosyl chloride to form the required 2,3-dichloro-4-acetyloxy or 4-tosyloxy-benzoic acid and the corresponding acyl chloride is subsequently formed in accordance with known procedures, for instance by reaction with thionyl chloride.
With respect to the compounds of formula VI, these are compounds falling within the scope of formula I above.
Indolizine derivatives are already known which have pharmacological effects capable of rendering them useful in the treatment of angina pectoris and cardiac arrhythmias.
In this connection, U.S. Pat. No. 4,103,012 can be cited which more particularly describes 2-ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine known under the non-proprietary name of butoprozine. In the British patent in question, antianginal properties are attributed to compounds described therein since they produce the following cardiovascular effects:
bradycardia
decrease in arterial pressure
.alpha.-antiadrenergic effect
.beta.-antiadrenergic effect
coronarodilating effect.
The increase of the blood-flow of the myocardium provoked by these compound is, in fact, only slight because their action does not last long: it is exerted for only a few minutes after an intravenous injection.
Furthermore, the compounds described in the aforesaid British patent only possess a weak .beta.-adrenergic antagonist action. This action is only slight at the minimum dose at which the other four cardiovascular effects cited above manifest themselves to a significant degree.
It has now been found quite surprisingly that by substituting in an appropriate manner with one or more methyl or methoxy groups or halogen atoms for example chlorine, bromine or iodine, dialkylaminoalkyloxybenzoyl indolizine derivatives, compounds are obtained which present a much broader spectrum of cardiovascular properties then the derivatives of U.S. Pat. No. 4,103,012 while showing less toxicity.
Thus, it has been possible to demonstrate that the indolizine derivatives of the invention can be regarded as powerful coronarodilators since they are capable of increasing the blood-flow to the myocardium to a marked degree and for a longer period of time than butoprozine. Compounds of the invention were also found to reduce cardiac frequency and arterial pressure in the animal.
Furthermore, the indolizine derivatives present a .beta.-antiadrenergic effect which is much more powerful than that of the derivatives of the aforesaid British patent. At the minimum dose required to produce bradycardia, a reduction in arterial pressure, .alpha.-antiadrenergic and coronarodilatory effects to a significant degree, the .beta.-antiadrenergic effect is, in fact, only slight in the case of the derivatives of the British patent in question whereas it is much stronger with the indolizine derivatives of the invention.
Moreover, the compounds of the invention reduce the consumption of oxygen by the myocardium calculated by multiplying the difference between the arterial and venous blood in oxygen by the coronary blood-flow. Thus, the compounds of the invention cause a considerable reduction in the arterio-venous difference which induces a decrease in the consumption of oxygen in spite of the increase of the coronary blood-flow.
Furthermore, unlike butoprozine, these beneficial effects on the consumption of oxygen are obtained with the compounds of the invention without any decrease in the contractility of the myocardium.
It has been further demonstrated that the derivatives of the invention are less toxic than the compounds of U.S. Pat. No. 4,103,012. Acute toxicity tests carried out by intravenous and oral routes in the animal have shown that the lethal doses are higher in the case of compounds of the invention than in the case of derivatives of the British patent in question.
Furthermore, higher blood levels can be obtained with compounds of the invention than with butoprozine.
Thus, it has been shown that the same oral dose of 1-bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine and of butoprozine administered to the dog induces a blood level which is three times higher with the compound of the invention than with butoprozine. Likewise, the compounds of the invention, when administered in long-term treatment per os in the dog have shown no cardiac toxicity as represented by ventricular arrhythmia which is not the case with butoprozine.
Finally, the indolizine derivatives of the invention exert a milder depressant action on the contractility of the myocardium than does butoprozine.
In man, an attack of angina pectoris is the painful consequence of a deficiency between the supply and the requirement in oxygen of the myocardium.
A compound can thus be active in the treatment of angina pectoris either by increasing the supply of oxygen or by decreasing the need of oxygen. Amongst the products commonly used in humans for treating angina pectoris, can be cited dipyridamole amongst the coronarodilators and amiodarone amongst the compounds which decrease the consumption of oxygen by the myocardium.
Comparative tests have, however, shown that the compounds of the invention are very superior to dipyridamole and amiodarone from several points of view.
In particular, it has been shown that dipyridamole does not decrease the consumption of oxygen by the myocardium and that amiodarone cannot be regarded as a coronarodilator.
The derivatives of the present invention exert their effect through both of these factors. Thus, they decrease the consumption of oxygen by the myocardium through their metabolic effects and also increase the coronary blood-flow in a long-lasting manner. Moreover, they are capable of preventing or curing not only arrhythmias induced by ischemia of the myocardium but also the auricular and ventricular arrhythmias of widely varying origins.
Thus, it appears that the halogenating, methoxylating or methylating of indolizine derivatives of U.S. Pat. No. 4,103,012 gives rise to compounds possessing a novel spectrum of pharmacological properties valuable in the treatment of cardiac deficiencies.
For instance:
the sites of the myocardium which are insufficiently irrigated can be nourished by means of the coronarodilating effect which can induce the development of an additional collateral circulation. This effect is valuable for the treatment of both anginal pain and the disturbances of rhythm consequent upon ischemia of the myocardium.
the antiadrenergic effect is also valuable for the treatment of both angina pectoris and cardiac arrhythmia in view of the important role played by hyperactivity of the sympathetic system in the etiology of these two cardiac diseases.
Since the physiological mediator of the sympathetic system is epinephrine, a compound which inhibits all the effects of epinephrine will probably be more active than a compound which only inhibits a part of these effects.
For this reason, the compounds of the present invention which inhibit both .alpha. and .beta. effects of epinephrine present an advantage over the derivatives of the U.S. Pat. No. 4,103,012 which practically only inhibit the .alpha. effects at the minimum dose at which the other pharmacological cardiovascular effects occur.
Amongst the compounds of the invention which have shown the most outstanding anti-anginal potentialities, the following may be cited:
1-Bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]indolizine
2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine
2-Ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine
2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine
2-Isopropyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizine
1-Bromo-2-phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine
1-Chloro-2-ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine
1-Chloro-2-n-butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine
1-Bromo-2-(4-chloro-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chlorobenzoyl]-indolizine
these compounds being in the form of the free base or of a pharmaceutically acceptable acid addition salt such as, for example, the hydrochloride or acid oxalate.
The results of pharmacological tests carried out in order to determine the cardiovascular properties of the compounds of the invention are given hereunder.
I. Antianginal properties
1. Effect on blood-flow to the myocardium.
This test was carried out in accordance with the technique described by R. CHARLIER & J. BAUTHIER in Arzneimittel--Forschung "Drug Research" 23, No. 19, 1305-1311 (1973).
It was undertaken on anaesthetized dogs which received the substance under study by intravenous route. The intensity of the maximum effect on blood-flow to the myocardium was expressed in percentage of the corresponding value before injection. The time required for the maximum effect to decrease by 50% represented the duration of the effect. This time was measured in minutes.
The following results were registered:
______________________________________ ##STR10## Time re- Max. quired Dose in- to de- (mg/ crease creaseX.sub.1 X.sub.2 X.sub.3 R R.sub.1 n kg) (%) to 50%______________________________________Br Br H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 10 60 90Br Br H CH.sub.3 n-C.sub.4 H.sub.9 3 10 125 40Br Br H n-C.sub.4 H.sub.9 n-C.sub. 3 H.sub.7 3 10 100 20Br Br H n-C.sub.3 H.sub.7 n-C.sub.3 H.sub.7 3 10 90 20Br Br H ##STR11## n-C.sub.3 H.sub.7 3 10 100 20Br Br H ##STR12## n-C.sub.4 H.sub.9 3 10 150 100H Br H ##STR13## n-C.sub.4 H.sub.9 3 10 90 25Br Br H CH.sub.3 n-C.sub.4 H.sub.9 3 10 77 45H Br H n-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 2 120 25H Br H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 5 70 60Br Cl H iso-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 5 133 20Cl H H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 10 60 30H Cl Cl iso-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 10 30 75H Cl Cl ##STR14## n-C.sub.4 H.sub.9 3 10 45 15H Cl Cl iso-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 10 30 75H Cl H ##STR15## n-C.sub.4 H.sub.9 3 10 30 45H Cl H ##STR16## n-C.sub.3 H.sub.7 3 8 45 25H Cl H iso-C.sub.3 H.sub.7 n-C.sub.3 H.sub.7 3 5 80 90Br Cl H ##STR17## n-C.sub.4 H.sub.9 3 10 90 90Br Cl H n-C.sub.4 H.sub.9 n-C.sub.3 H.sub.7 3 10 40 90Br Cl H ##STR18## n-C.sub.3 H.sub.7 3 10 50 50H Cl H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 5 75 30H Cl H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 6 70 45Br Cl H ##STR19## n-C.sub.3 H.sub.7 3 10 63 45H Cl H ##STR20## n-C.sub.4 H.sub.9 3 10 60 25Br Cl H n-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 2.5 50 60Cl Cl H ##STR21## n-C.sub.3 H.sub.7 3 8 70 90Cl Cl H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 1 40 15Cl Cl H C.sub.2 H.sub.5 n-C.sub.3 H.sub.7 3 3.3 60 25Cl H H ##STR22## n-C.sub.3 H.sub.7 3 5 50 40Butoprozine 10 120 4Amiodarone 10 36 7______________________________________
These results clearly show that the compounds of the invention are more valuable than butoprozine and amiodarone as regards the effects on bloodflow to the myocardium.
2. Antiadrenergic effects
The purpose of this test was to determine the capacity of the compounds under study to reduce epinephrine-increased blood-pressure (anti-.alpha. effect) and epinephrine-accelerated heart rate (anti-.beta. effect) in the dog previously anaesthetized with pentobarbital and atropinized.
Anti-.alpha. effect
For each dog, the dose of epinephrine was first determined which provoked a reproductible increase by about 100 mm. Hg in the arterial pressure (between 5 and 10 .mu.g/kg).
After that, the dose of epinephrine so determined was administered followed by a dose by intravenous route of the compound to be studied. The percentage of reduction of the hypertension provoked by the compound under study in comparison with the hypertension previously obtained (about 100 mm.Hg) was then registered.
Anti-.beta. effect
During the same test as that described above, the epinephrine provoked a reproductible increase in the heart-rate of about 70 beats/min. The percentage of reduction of the epinephrine-induced acceleration of heart-rate produced by the compound under study in comparison with the tachycardia previously measured (about 70 beats) was then registered. In both cases, the results were expressed as follows:
+ for a <50%-reduction of the increase in pressure or cardiac frequency
++ for a .gtoreq.50%-reduction of the increase in pressure or cardiac frequency
+++ for a subtotal reduction of the increase in pressure or cardiac frequency
The following results were registered:
__________________________________________________________________________ Dose Anti- Anti- (mg/ .alpha. ef- .beta. ef-X.sub.1 X.sub.2 X.sub.3 R R.sub.1 n kg) fect fect__________________________________________________________________________Br Br H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 10 +++ +++Br H H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 6 +++ +++Br Br H ##STR23## n-C.sub.4 H.sub.9 3 10 +++ ++Br Br Br n-C.sub.3 H.sub.7 n-C.sub.3 H.sub.7 3 10 +++ +++H Br H ##STR24## n-C.sub.4 H.sub.9 3 10 +++ ++H Br H C.sub. 2 H.sub.5 n-C.sub.4 H.sub.9 3 7.5 +++ ++H Br H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 5 +++ ++H Br H n-C.sub.4 H.sub.9 n-C.sub.3 H.sub.7 3 5 +++ ++Br Br H CH.sub.3 n-C.sub.4 H.sub.9 3 5 +++ ++ 10 +++ +++H Br H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 5 +++ ++ 10 +++ +++H Br H iso-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 6 +++ ++Cl H H n-C.sub.4 H.sub.9 N-C.sub.4 H.sub.9 3 10 +++ ++Cl H H n-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 7.5 +++ +++H Cl H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 10 +++ ++H Cl H C.sub.2 H.sub.5 n-C.sub.3 H.sub.7 3 10 ++ ++H Cl H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 6 +++ ++H Cl Cl iso-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 10 ++ ++H Cl H ##STR25## n-C.sub.4 H.sub.9 3 10 ++ ++H Cl H iso-C.sub.3 H.sub.7 n-C.sub.3 H.sub.7 3 10 ++ ++Br Cl H ##STR26## n-C.sub.4 H.sub.9 3 10 ++ +++Br Cl H C.sub.2 H.sub.5 n-C.sub.3 H.sub.7 3 7.8 ++ ++Br Cl H ##STR27## n-C.sub.3 H.sub.7 3 10 ++ ++Br Cl H n-C.sub.4 H.sub.9 n-C.sub.3 H.sub.7 3 10 ++ +Br Cl H ##STR28## n-C.sub.4 H.sub.9 3 10 ++ ++Br Cl H ##STR29## n-C.sub.3 H.sub.7 3 10 ++ ++Br Cl H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 10 +++ +++Br Cl H ##STR30## n-C.sub.3 H.sub.7 3 10 +++ ++H Cl H ##STR31## n-C.sub.4 H.sub.9 3 10 ++ +Br Cl H ##STR32## n-C.sub.3 H.sub.7 3 10 +++ +++Br Cl H n-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 10 +++ +++Cl Cl H ##STR33## n-C.sub.3 H.sub.7 3 8 +++ ++Cl Cl H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 0.5 +++ +Cl Cl H C.sub.2 H.sub.5 n-C.sub.3 H.sub.7 3 3.3 +++ +++Cl H H ##STR34## n-C.sub.4 H.sub.9 3 10 +++ ++Cl H H ##STR35## n-C.sub.3 H.sub.7 3 5 ++ +Cl H H ##STR36## n-C.sub.4 H.sub.9 3 5 +++ +++H Br H iso-C.sub.3 H.sub.7 n-C.sub.3 H.sub.7 3 7.5 +++ ++Br OCH.sub.3 H CH.sub.3 n-C.sub.3 H.sub.7 3 7.5 ++ ++Cl Cl Cl C.sub.2 H.sub.5 n-C.sub.3 H.sub.7 3 10 ++ ++Cl Cl Cl ##STR37## n-C.sub.4 H.sub.9 3 10 +++ ++Cl Br H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 10 +++ +++Cl Br Br ##STR38## n-C.sub.3 H.sub.7 3 10 + +Br Cl Cl ##STR39## n-C.sub.3 H.sub.7 3 10 ++ ++Br Cl Cl ##STR40## n-C.sub.4 H.sub.9 3 10 ++ ++Br CH.sub.3 CH.sub.3 CH.sub.3 n-C.sub.4 H.sub.9 3 10 +++ ++H Br Br ##STR41## n-C.sub.3 H.sub.7 3 10 +++ +++H Br Br ##STR42## n-C.sub.4 H.sub.9 3 10 ++ ++OCH.sub.3 Cl H ##STR43## n-C.sub.3 H.sub.7 3 10 +++ +++OCH.sub.3 OCH.sub.3 H ##STR44## n-C.sub.3 H.sub.7 3 10 ++ ++H OCH.sub.3 H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 10 +++ ++Br OCH.sub.3 H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 5 ++ ++Br OCH.sub.3 H CH.sub.3 n-C.sub.4 H.sub.9 3 7 ++ ++Br OCH.sub.3 H CH.sub.3 n-C.sub.3 H.sub.7 3 7.5 ++ ++Butoprozine 5 +++ +Amiodarone 10 ++ ++__________________________________________________________________________
These results again show that the compounds of the invention are more valuable than those of the prior art.
II. Anti-arrhythmic properties
These properties were demonstrated after administration of the compound under study by intragastric route to mice using the LAWSON test (J. Pharmac. Exp. Therap. 1968, 160 (1) p. 22-31).
The arrhythmia was provoked by making the animals inhale chloroform to total asphyxia and by later observing the ventricular rhythm. The dose of compound protecting 50% of the animals against ventricular fibrillation i.e. the AD.sub.50 was then recorded. The following results were registered:
______________________________________ AD.sub.50X.sub.1 X.sub.2 X.sub.3 R R.sub.1 n (mg/kg)______________________________________Br Br H CH.sub.3 n-C.sub.4 H.sub.9 3 180H Br H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 170Butoprozine 270______________________________________
III Toxicity
Acute toxicity
Acute toxicity tests were carried out on rats and mice. The following results were registered in comparison with butoprozine. The compounds of the invention were used in oxalate acid form except those marked (x) which were tested in hydrochloride form.
______________________________________X.sub.1 X.sub.2 X.sub.3 R R.sub.1 n LD.sub.50 (mg/kg)______________________________________(a) By intravenous route to rats(*) Br Br H CH.sub.3 n-C.sub.4 H.sub.9 3 70H Br H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 60(*) H Cl H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 65H Cl Cl iso-C.sub.3 H.sub.7 n-C.sub.4 H.sub.9 3 >100(*) Br Cl H ##STR45## n-C.sub.4 H.sub.9 3 >100(*) H Cl H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 >50 (LD.sub.0 >50 mg/kg)Cl Cl H C.sub.2 H.sub.5 n-C.sub.4 H.sub.9 3 50Cl H H n-C.sub.4 H.sub.9 n-C.sub.4 H.sub.9 3 50Br Cl H ##STR46## n-C.sub.4 H.sub.9 3 >100 (LD.sub.0 >100 mg/kg)Butoprozine 22(b) By intravenous route to mice(*) Br Br H CH.sub.3 n-C.sub.4 H.sub.9 3 50Butoprozine 25(c) By intragastric route to mice(*) Br Br H CH.sub.3 n-C.sub.4 H.sub.9 3 >5000 (LD.sub.0 >5000 mg/kg)Butoprozine 1600______________________________________
These results show that the compounds of the invention are far less toxic than butoprozine.
Cardiac tolerance and general toxicity in long-term toxicity tests
1-Bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine hydrochloride, 2-n-butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine hydrochloride and 2-n-butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzyl]-indolizine hydrochloride provoked neither ventricular arrhythmia nor mortality at the dose of 200 mg/kg/day by oral route in the dog.
As against this, butoprozine was found to provok ventricular arrhythmia with a dose as low as 50 mg/kg/day by oral route in the dog, the lethal dose of this compound being between 50 and 100 mg/kg/day.
It will be appreciated that for therapeutic use the compounds of the invention will normally be administered in the form of a pharmaceutical or veterinary composition, which may be in a dosage unit form appropriate to the desired mode of administration.
Thus the pharmaceutical or veterinary composition may be in a dosage unit form suitable for oral administration, for example a coated or uncoated tablet, a hard- or soft-gelatin capsule, a packaged powder, or a discrete amount of a suspension, or a syrup. The composition may alternatively take the form of a suppository for rectal administration, or of a solution or suspension for parenteral administration.
When in dosage unit form, the composition may contain for example from 15% to 50% by weight of the active ingredient per dosage unit for oral administration, from 3% to 15% of the active ingredient per dosage unit for rectal administration and from 3% to 5% of the active ingredient per dosage unit for parenteral administration.
Irrespective of the form which the composition takes, the pharmaceutical or veterinary composition of the invention will normally be prepared by associating at least one of the compounds of formula I or a pharmaceutically acceptable acid addition salt thereof with an appropriate pharmaceutical carrier or excipient therefor, for example one or more of the following substances: milk sugar, starches, talc, magnesium stearate, polyvinylpyrrolidone, alginic acid, colloidal silica, distilled water, benzyl alcohol or flavouring agents.

The following Examples illustrate the invention:
EXAMPLE 1
1-Bromo-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine and its acid oxalate
(a) 1-Bromo-2-ethyl-3-[4-(3-bromopropyl)-oxy-3-bromo benzoly]-indolizine
A mixture of 7.7 g (0.018 mol) of 1-bromo-2-ethyl-3-(3-bromo-4-hydroxybenzoyl) indolizine, 5 g (0.036 mol) of anhydrous potassium carbonate and 50 ml of methyl ethyl ketone was stirred in a flask for 30 minutes. To this reaction medium 14.4 g (0.072 mol) of 1,3-dibromo-propane were then added and the mixture was refluxed for 20 hours. After cooling, the mineral salts were filtered out and washed with acetone. The solvents were evaporated off together with the 1,3-dibromo-propane in excess. In this manner, 13.2 g of a product were obtained which were purified by elution chromatography on silica using benzene as elution agent. A first fraction of an unknown product was obtained and then a second fraction of 8 g of the desired product.
In this manner, 1-bromo-2-ethyl-3-[4-(3-bromopropyl)-oxy-3-bromo-benzoyl]-indolizine was obtained in a yield of 83.6%.
M.P.: 105.degree.-106.degree. C.
(b) 1-Bromo-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]indolizine
A mixture of 2.2 g (0.004 mol) of 1-bromo-2-ethyl-3-[4-(3-bromopropyl)-oxy-3-bromo-benzoyl]-indolizine, 1.2 g (0.012 mol) of di-n-propylamine and 25 ml of toluene were refluxed in a flask for 20 hours. After cooling, the reaction medium was washed twice with 10 ml of water and the solvent was evaporated off under vacuum. Thus, 2.5 g of a residue were obtained which were purified by elution chromatography on silica using ethyl acetate as eluent. By this method, 2.4 g of 1-bromo-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine in free base form were obtained.
(c) 1-Bromo-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate.
The base previously obtained was dissolved in 30 ml of ethyl ether and then reacted with 0.55 g of oxalic acid in 70 ml of ethyl ether to give 2.4 g of the desired salt in crude form. From this quantity, 2.0 g of pure 1-bromo-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate were obtained by recristallization from 75 ml of isopropanol.
Yield: 75%
M.P.: 139.degree.-140.degree. C.
EXAMPLE 2
1-Bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]indolizine and salts thereof.
Into a 1-l flask, a mixture of 180 ml of water, 180 ml of toluene, 73 g (0.178 mol) of 1-bromo-2-methyl-3-(3-bromo-4-hydroxy-benzoyl)-indolizine, 42.7 g (0.21 mol) of 1-di-n-butylamino-3-chloro-propane and 34.5 g of potassium carbonate was introduced while stirring. The reaction medium was heated under reflux for 20 hours. After cooling to room-temperature, the aqueous phase was decanted and the toluene layer was washed three times, each time with 200 ml of water. The toluene solution was transferred to a flask and, under atmospheric pressure, toluene was distilled off to dryness and the residue so obtained was cooled. In this manner, crude 1-bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine was obtained in free base form. The following salts of this compound were then prepared:
(a) hydrochloride
To the free base previously obtained, a solution of 8 g of hydrochloric acid in 61 ml of ethyl acetate was added. The precipitate so formed, i.e. 104 g, was suction-filtered, washed with ethyl acetate and recrystallized from 500 ml of isopropanol. In this manner, 93 g of 1-bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine hydrochloride were obtained.
Yield: 84.7% M.P.: 172.degree. C.
(b) acid oxalate
To an ethereal solution of the base previously obtained, an equimolecular solution of oxalic acid in ethyl ether was added. The salt so obtained was recrystallized from isopropanol.
In this manner, 1-bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate was obtained.
M.P.: 89.degree.-90.degree. C.
EXAMPLE 3
2-Methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate
Into a 250 ml-flask, a mixture of 4 g (0.01 mol) of 2-methyl-3-(3,5-dibromo-4-hydroxy-benzoyl)-indolizine and 150 ml of acetone was introduced. After the indolizine dissolved, there were added 4 g of anhydrous potassium carbonate and 2.2 g of di-n-butylaminopropyl chloride.
While stirring, the reaction mixture was refluxed for 16 hours. After cooling to room-temperature, the mineral salts were filtered out and washed with acetone on the filter. The acetone was then distilled off under reduced pressure using a rotatory evaporator and the oily residue was dissolved in about 100 ml of ethyl acetate. The medium was filtered on a filter and 1.5 g of anhydrous oxalic acid was added to the filtrate. The reaction medium was allowed to stand and the oxalate which crystallized was filtered out, washed on the filter with ethyl acetate and dried under vacuum.
In this manner, 6.2 g of 2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate were obtained.
Yield: 92.7%
M.P.: 96.degree. C.
EXAMPLE 4
1-Bromo-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizine acid oxalate
Into a 250 ml flask were introduced 2.8 g (0.005 mol) of 2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizine acid oxalate and 80 ml of dioxan. The reaction mixture was stirred and after the indolizine had dissolved, 0.8 g of anhydrous sodium acetate was added. Through a dropping-funnel was added, dropwise and under vigorous stirring, a solution of 0.8 g of bromine in 20 ml of dioxan. The temperature was maintained at about 20.degree. C. during the introduction of the bromine.
After the medium had been stirred for 2 hours at room-temperature, the dioxan was distilled off under vacuum with a rotatory evaporator. The solid residue was dissolved in water, made alkaline with a sodium hydroxide solution and extracted with chloroform. The chloroformic solution was washed three times with water and the chloroform was distilled off under reduced pressure. The oily residue so obtained was taken up in dry ethyl ether and after filtration on a filter the acid oxalate was formed.
In this manner, 1.8 g of 1-bromo-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizine acid oxalate was obtained after recrystallization from ethyl acetate.
Yield: 55.8%
M.P.: 135.degree. C.
Using the appropriate starting-products, the following compounds were prepared by utilizing the various processes described in the foregoing Examples.
__________________________________________________________________________Compounds M.P. .degree.C.__________________________________________________________________________1-Bromo-2-ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine acid oxalate 107-108 (isopropanol)1-Bromo-2-(4-bromo-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine 92-94acid oxalate (isopropanol)1-Chloro-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine acid oxalate 92-93 (isopropanol)1-Chloro-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-benzoyl]-indolizine acid oxalate 162 (isopropanol)1-Chloro-2-n-propyl-3-[4-(3-di-n-butylamino-propyl)-oxy-benzoyl]-indolizine acid oxalate 111-112 (isopropanol)1-Chloro-2-n-butyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-benzoyl]-indolizine acid oxalate 106-108 (isopropanol)1-Chloro-2-phenyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-benzoyl]-indolizine acid oxalate 161-162 (isopropanol)1-Chloro-2-(4-chloro-phenyl)-3-[4-(3-di-n-butyla-minopropyl)-oxy-benzoyl]-indolizine acid oxalate 158-159 (methanol)1-Chloro-2-phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine acid oxalate 160-161 (methanol)1-Chloro-2-n-butyl-3-[4-(6-di-n-butylaminohexyl)-oxy-benzoyl]-indolizine acid oxalate 80-82 (benzene)2-Methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 141-143 (10/1 ethyl acetate/ isopropanol)2-Ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 163 (isopropanol)2-Ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 98-99 (isopropanol)2-n-Propyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 145 (isopropanol)2-n-Propyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 113-115 (isopropanol)2-Isopropyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 105-107 (benzene)2-n-Butyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 136-137 (isopropanol)2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 86-87 (isopropanol)2-Phenyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 148-149 (isopropanol)2-Phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 129-130 (isopropanol)2-(4-Fluoro-phenyl)-3-[4-(3-di-n-butylamino-propyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 110 (isopropanol)2-(4-Chloro-phenyl)-3-[4-(3-di-n-propylamino-propyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 163-164 (methanol)2-(4-Chloro-phenyl)-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 130-140 (isopropanol)2-(3-Bromo-phenyl)-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 142-143 (isopropanol)2-(4-Bromo-phenyl)-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 147-148.5 (methanol)2-(4-Methoxy-phenyl)-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 169 (isopropanol)2-Isopropyl-3-[4-(5-di-n-butylaminopentyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 80-82 (benzene)2-Isopropyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 179 (isopropanol)2-Methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 141-143 (isopropanol)2-Ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 161-162 (methanol)2-Ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 116-117 (isopropanol)2-Isopropyl-3-[ 4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 115-117 (isopropanol)2-Isopropyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 168-169 (methanol)2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 84-85 and 107-109 (isopropanol)2-n-Butyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 130-131 (isopropanol)2-Phenyl-3-[2-Phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 121-122 (isopropanol)2-Phenyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 157-159 (methanol)2-(4-Methyl-phenyl)-3[4-(3-di-n-propylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 134-135 (isopropanol)2-(4-Bromo-phenyl)-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 154-155 (methanol)2-(4-Bromo-phenyl)-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 134-135 (isopropanol)2-(3-Bromo-phenyl)-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 92-93 (isopropanol)2-(3-Bromo-phenyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 148-150 (isopropanol)2-(4-Chloro-phenyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 116-118 (isopropanol)2-(4-Chloro-phenyl)-3-[4-(3-di-n-propylamino-propyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate159-160 (methanol)1-Bromo-2-methyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 89-90 (isopropanol)1-Bromo-2-methyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 164-165 (isopropanol/ methanol)1-Bromo-2-ethyl-3-[4-(2-dimethylaminoethyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 164-165 (dichlorethane)1-Bromo-2-ethyl-3-[4-(3-dimethylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 150-151 (dichlorethane)1-Bromo-2-ethyl-3-[4-(2-diethylaminoethyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 168-169 (dichlorethane)1-Bromo-2-ethyl-3-[4-(3-diethylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 140-141.5 (isopropanol)1-Bromo-2-ethyl-3-[4-(2-di-n-propylaminoethyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 163-164 (isopropanol)1-Bromo-2-ethyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 139-140 (isopropanol)1-Bromo-2-ethyl-3-[4-(2-di-n-butylaminoethyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 164-165 (isopropanol)1-Bromo-2-ethyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 101-101.5 (isopropanol)1-Bromo-2-n-propyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 92 (benzene)1-Bromo-2-n-propyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 132-136 (isopropanol)1-Bromo-2-n-butyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 151-152 (2/1 isopropanol/ methanol)1-Bromo-2-n-butyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 101-103 (isopropanol)1-Bromo-2-phenyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 169-170 (1/1 methanol/iso- propanol)1-Bromo-2-phenyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 167-169 (isopropanol)1-Bromo-2-(4-methoxy-phenyl)-3-[4-(3-di-n-buty-laminopropyl)-oxy-3-bromo-benzoyl]-indolizineacid oxalate 178-179 (methanol)1-Bromo-2-(4-methyl-phenyl)-3-[4-(3-di-n-buty-laminopropyl)-oxy-3-bromo-benzoyl]-indolizineacid oxalate 169-170.5 (1/1 isopropanol/ (methanol)1-Bromo-2-(4-fluoro-phenyl)-3-[4-(3-di-n-buty-laminopropyl)-oxy-3-bromo-benzoyl]-indolizineacid oxalate 170-171 (methanol)1-Bromo-2-(3-bromo-phenyl)-3-[4-(3-di-n-buty-laminopropyl)-oxy-3-bromo-benzoyl]-indolizineacid oxalate 172-173 (methanol)1-Bromo-2-n-butyl-3-[4-(4-di-n-butylamino-butyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 118-120 (isopropanol)1-Chloro-2-ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 115-117 (isopropanol)1-Chloro-2-ethyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 137-138 (isopropanol)1-Chloro-2-(3-bromo-phenyl)-3-[4-(3-di-n-buty-laminopropyl)-oxy-3-chloro-benzoyl]-indolizineacid oxalate 171-172 (methanol)1-Chloro-2-(3-bromo-phenyl)-3-[4-(3-di-n-propyl-aminopropyl)-oxy-3-chloro-benzoyl]-indolizineacid oxalate 194-195 (methanol)1-Chloro-2-ethyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3,5-dichloro-benzoyl] -indolizineacid oxalate 141 (ethyl acetate)1-Chloro-2-ethyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3,5-dichloro-benzoyl] -indolizineacid oxalate 129 (ethyl acetate)1-Chloro-2-phenyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3,5-dichloro-benzoyl] -indolizineacid oxalate 156 (isopropanol)1-Chloro-2-phenyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3,5-dichloro-benzoyl]dichloro-benzoyl]-indolizineacid oxalate 136 (isopropanol/heptane)1-Bromo-2-methyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 110-112 (isopropanol)1-Bromo-2-methyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 108-110 (isopropanol)1-Bromo-2-ethyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 136.5-138 (isopropanol)1-Bromo-2-ethyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 103-105 (isopropanol)1-Bromo-2-n-propyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 95-96 (isopropanol)1-Bromo-2-isopropyl-3-[4-(3-di-n-butylamino-propyl)-oxy-3-chloro-benzoyl]-indolizineacid oxalate 116 (isopropanol)1-Bromo-2-n-butyl-3-[4-(3-di-n-propylamino-propyl)-oxy-3-chloro-benzoyl]-indolizineacid oxalate 159-160 (methanol)1-Bromo-2-n-butyl-3-[4-(3-di-n-propylamino-propyl)-oxy-3-chloro-benzoyl]-indolizineacid oxalate 101-103 (isopropanol)1-Bromo-2-phenyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 167.5-169 (methanol)1-Bromo-2-phenyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3-chloro-benzoyl]-indolizine acidoxalate 169-170 (methanol)1-Bromo-2-(4-chloro-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 160-161 (isopropanol)1-Bromo-2-(4-chloro-phenyl)-3-[4-(3-di-n-propylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 184-185 (methanol)1-Bromo-2-(4-bromo-phenyl)-3-[4-(3-di-n-propylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 176-177 (methanol)1-Bromo-2-(4-bromo-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 168-169 (isopropanol)1-Bromo-2-(3-bromo-phenyl)-3-[4-(3-di-n-propylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 200-201 (methanol)1-Bromo-2-(3-bromo-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 170.5-172 (methanol)1-Chloro-2-ethyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3-bromo-benzoyl]-indolizine acidoxalate 104-105 (isopropanol)1-Chloro-2-ethyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 157 (isopropanol)1-Chloro-2-ethyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 140 (isopropanol)1-Chloro-2-phenyl-3-[4-(3-di-n-propylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indo-lizine acid oxalate 172 (isopropanol)1-Chloro-2-phenyl-3-[4-(3-di-n-butylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 146 (isopropanol)2-Methyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine sesqui-oxalate 136 (ethyl acetate)2-Ethyl-3-[4-(3-dimethylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate 148 (ethyl acetate)2-Ethyl-3-[4-(2-diethylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate 171 (ethanol)2-Ethyl-3-[4-(3-diethylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine hydrochloride 191 (50/50 ethyl acetate/ acetone)2-Ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizinehydrochloride 166 (ethyl acetate)2-Ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizinehydrochloride 157 (ethyl acetate)2-n-Propyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizinehydrochloride 145 (ethyl acetate)2-n-Propyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acidoxalate 107 (ethyl acetate)2-Isopropyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acidoxalate 126 (ethyl acetate/ ethanol)2-Isopropyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acidoxalate 86 (ethyl acetate/ethyl ether)2-n-Butyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acidoxalate 146 (ethyl acetate)2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 110 (ethyl acetate)2-Phenyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acidoxalate 142 (ethyl acetate/ ethanol)2-Phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acidoxalate 86 (ethyl acetate)2-(4-Fluoro-phenyl)-3-[4-(3-di-n-propylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 166 (ethyl acetate/ ethanol)2-(4-Fluoro-phenyl)-3-[4-(3-di-n-butylami-nopropyl)-oxy-3,5-dibromo-benzoyl]-indoli-zine acid oxalate 152 (isopropanol)2-(4-Chloro-phenyl)-3-[4-(3-di-n-propyla-minopropyl)-oxy-3,5-dibromo-benzoyl]-indo-lizine acid oxalate 190 (ethyl acetate)2-(4-Chloro-phenyl)-3-[4-(3-di-n-butylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 88 (ethyl acetate)2-(3,4-Dichloro-phenyl)-3-[4-(3-di-n-propyla-minopropyl)-oxy-3,5-dibromo-benzoyl]-indolizinesesquioxalate 114 (ethyl acetate/iso- propanol)2-(3,4-Dichloro-phenyl)-3-[4-(3-di-n-butyl-aminopropyl)-oxy-3,5-dibromo-benzoyl]-indo-lizine acid oxalate 95 (ethyl acetate/iso- propanol)2-(3-Bromo-phenyl)-3-[4-(3-di-n-propylami-nopropyl)-oxy-3,5-dibromo-benzoyl]-indoli-zine acid oxalate 136 (ethanol)2-(3-Bromo-phenyl)-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3,5-dibromo-benzoyl]-indolizine acidfumurate 122 (ethyl acetate)2-(4-Methyl-phenyl)-3-[4-(3-di-n-propylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 126 (ethyl acetate/ isopropanol)2-(4-Methyl-phenyl)-3-[4-(3-di-n-butylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 90 (ethyl acetate)2-(4-Methoxy-phenyl)-3-[4-(3-di-n-propylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indolizineacid oxalate 167 (acetone)2-(4-Methoxy-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate pasty at about 70.degree. C. (ethanol/ethyl ether)2-Methyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 145 (ethyl acetate/ethanol)2-Methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 95 (ethyl acetate/ethyl ether)2-Ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 100 (pasty) (ethyl acetate)2-Ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 95 (ethyl acetate)2-n-Propyl-3-[4-(3-di-n-propylaminopro-pyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 142 (isopropanol)2-n-Propyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 114 (isopropanol)2-Isopropyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 86 (ethyl acetate)2-Isopropyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizine acidoxalate 90 (ethyl acetate)2-Phenyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 146 (ethanol)2-Phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate pasty at about 90.degree. C. (ethyl acetate)2-(4-Bromo-phenyl)-3-[4-(3-di-n-propyl-aminopropyl)-oxy-3,5-dichloro-benzoyl]-indolizine acid oxalate 174 (ethyl acetate)2-(4-Bromo-phenyl)-3-[4-(3-di-n-butyla-minopropyl)-oxy-3,5-dichloro-benzoyl]-indolizine acid oxalate 133 (ethyl acetate)1-Bromo-2-methyl-3-[4-(3-di-n-butyla-minopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate 141-143 (isopropanol)1-Bromo-2-n-propyl-3-[4-(3-di-n-propyla-minopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate 138-139 (isopropanol)1-Bromo-2-ethyl-3-[4-(3-di-n-butylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indoli-zine acid oxalate 131-132.5 (isopropanol)1-Bromo-2-phenyl-3-[4-(3-di-n-butylamino-propyl)-oxy-3,5-dibromo-benzoyl]-indoli-zine acid oxalate 160-161 (isopropanol)1-Bromo-2-(4-methyl-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate 105-106 (isopropanol)1-Bromo-2-(4-bromo-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate 148-149 (isopropanol)1-Bromo-2-(3,4-dichloro-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-ben-zoyl]-indolizine acid oxalate 196-197 (isopropanol)1-Bromo-2-(3-chloro-4-methyl-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine acid oxalate 168-169 (isopropanol)1-Bromo-2-ethyl-3-[4-(3-di-n-butylaminopro-pyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 134 (isopropanol)1-Bromo-2-phenyl-3-[4-(3-di-n-propylamino-propyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 145 (ethyl acetate)1-Bromo-2-phenyl-3-[4-(3-di-n-butylamino-propyl)-oxy-3,5-dichloro-benzoyl]-indolizineacid oxalate 106 (ethyl acetate)2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine hydrochloride 113-113.5 (ethyl acetate)2-n-Propyl-3-[4-(3-diethyl-aminopropyl)-oxy-3,5-dibromo-benzoyl]-indolizine hydrochloride 154 (80/20 ethyl acetate/ acetone)2-Ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine hydrochloride 132-133 (ethyl acetate/isopropa- nol)2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine hydrochloride 104 (ethyl acetate)1-Bromo-2-phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine hydrochloride 156-1571-Chloro-2-(4-chloro-phenyl)-3-[ 4-(3-di-n-propyl-aminopropyl)-oxy-benzoyl]-indolizine acid oxalate 168-169 (methanol)1-Methoxy-2-phenyl-3-[4-(3-di-n-propylaminopropyl)-oxy-benzoyl]-indolizine acid oxalate 117 (ethyl acetate)1-Methoxy-2-phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine acid oxalate 80 (ethyl acetate)1-Methoxy-2-phenyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 172 (ethyl acetate)1-Methoxy-2-phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-chloro-benzoyl]-indolizine acid oxalate 120 (ethyl acetate)1-Methoxy-2-phenyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 160 (ethyl acetate)1-Methoxy-2-phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 78 (ethyl acetate)1-Methoxy-2-phenyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 148 (ethyl acetate)1-Methoxy-2-phenyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 78 (ethyl acetate)1-Methoxy-2-(4-fluoro-phenyl)-3-[4-(3-di-n-propylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 79 (ethyl acetate)2-Methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 159 (isopropanol)2-Methyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 171 (methanol)2-Ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 88 (ethyl acetate)2-Ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 121-122 (ethyl acetate)1-Bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 87-90 (benzene)1-Bromo-2-methyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 139-141 (isopropanol)1-Bromo-2-ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 111 (benzene)1-Bromo-2-ethyl-3-[4-(3-di-n-propylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 149 (isopropanol)2-(4-Fluoro-phenyl)-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methoxy-benzoyl]-indolizine acid oxalate 85 (ethyl acetate)2-Methyl-3-[4-(3-di-n-butylaminopropyl)-oxy3-methyl-benzoyl]-indolizine acid oxalate 149 (isopropanol)2-Ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methyl-benzoyl]-indolizine acid oxalate 133 (ethyl acetate)1-Methyl-2-n-propyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 127 (ethyl acetate)2-Isopropyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methyl-benzoyl]-indolizine acid oxalate 91 (isopropanol)2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-methyl-benzoyl]-indolizine acid oxalate 89 (isopropanol)1-Methyl-2-n-butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 99 (ethyl acetate)1-Methyl-2-ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo-benzoyl]-indolizine acid oxalate 117 (ethyl acetate)1-Bromo-2-methyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dimethyl-benzoyl]-indolizine acid oxalate 120-122 (isopropanol)1-Iodo-2-ethyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3,5-dimethyl-benzoyl]-indolizine acid oxalate 115 (ethyl acetate)2-Ethyl-3-[2,3-dichloro-4-(3-di-n-propylamino-propyl)-oxy-benzoyl]-indolizine 117 (heptane)2-Ethyl-3-[2,3-dichloro-4-(3-di-n-butylamino-propyl)-oxy-benzoyl]-indolizine 95 (heptane)2-Phenyl-3-[2,3-dichloro-4-(3-di-n-propylamino-propyl)-oxy-benzoyl]-indolizine 99 (heptane)2-Phenyl-3-[2,3-dichloro-4-(3-di-n-butylamino-propyl)-oxy-benzoyl]-indolizine 87 (heptane)2-(4-Fluoro-phenyl)-3-[2,3-dichloro-4-(3-di-n-propylaminopropyl)-oxy-benzoyl] 9 -indolizine 119 (heptane)2-(4-Fluoro-phenyl)-3-[2,3-dichloro-4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine 95-96 (heptane)1-Bromo-2-ethyl-3-[2,3-dichloro-4-(3-di-n-butylaminopropyl)-oxy-benzoyl]-indolizine acidoxalate 164 (isopropanol)1-Bromo-2-phenyl-3-[2,3-dichloro-4-(3-di-n-propylaminopropyl)-oxy-benzoyl]-indolizine acidoxalate 171 (isopropanol)1-Chloro-2-phenyl-3-[2,3-dichloro-4-(3-di-n-propylaminopropyl)-oxy-benzoyl]-indolizine 136 (heptane)2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-iodo-benzoyl]-indolizine acid oxalate 90-92 (ethyl acetate)__________________________________________________________________________
EXAMPLE 5
In accordance with known pharmaceutical techniques soft-gelatin capsules containing the following ingredients, were prepared:
______________________________________Ingredient mg______________________________________2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo/or 3-chloro-benzoyl]-indolizinehydrochloride 100Starches 99.5Colloidal silica 0.5 200.0______________________________________
EXAMPLE 6
In accordance with known pharmaceutical techniques, injectable solutions containing the following ingredients, were prepared:
______________________________________Ingredient mg______________________________________2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo/or 3-chloro-benzoyl]-indolizinehydrochloride 150Polysorbate 80 150Benzyl alcohol 75Water to 3 ml______________________________________
EXAMPLE 7
In accordance with known pharmaceutical techniques, suppositories containing the following ingredients, were prepared:
______________________________________Ingredient mg______________________________________2-n-Butyl-3-[4-(3-di-n-butylaminopropyl)-oxy-3-bromo/or 3-chloro-benzoyl]-indolizinehydrochloride 100Mixture of mono- and di-glycerides of saturatedacids (C.sub.12 to C.sub.18) 1400 1500______________________________________

Indolizine derivatives and process for preparing the same

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