Indolyl-substituted phenylacetic acid derivatives

Abstract

The indolyl-substituted phenylacetic acid derivatives are prepared by reacting the corresponding phenylacetic acids with the required amines. The indolyl-substituted phenylacetic acid derivatives are suitable as active compounds in medicaments, in particular in antiarteriosclerotic medicaments.

Description

[0001] The present invention relates to indolyl-substituted phenylacetic acid derivatives, processes for their preparation and their use as medicaments, in particular as antiatherosclerotic medicaments.

[0002] Phenylacetic acid derivatives with heterocyclic substituents and substituted imidazo[4,5-b]pyridines and benzimidazoles are known from the publications DOS 42 00 954 and DOS 43 02 956. Benzimidazole derivatives having a PAF-antagonistic action are furthermore described in the publication U.S. Pat. No. 5,314,880.

[0003] The compounds according to the invention are partly covered by the scope of meaning of these publications.

[0004] It is known that increased blood levels of triglycerides (hypertriglyceridaemia) and cholesterol (hypercholesterolaemia) are associated with the origin of atherosclerotic changes to the vascular walls and coronary heart diseases.

[0005] A significantly increased risk of the development of coronary heart diseases furthermore exists if these two risk factors occur in combination, which in turn is accompanied by excessive production of apolipoprotein B-100. There is therefore still a great need to provide active medicaments for combating atherosclerosis and coronary heart diseases.

[0006] The present invention relates to indolyl-substituted phenylacetic acid derivatives of the general formula (I) 1

[0007] in which

[0008] D represents a radical of the formula 2

[0009] wherein

[0010] T denotes a nitrogen atom or the —CH group,

[0011] R6, R7, R10 and R11 are identical or different and denote hydrogen, trifluoromethyl, halogen or straight-chain or branched alkyl or alkoxy having in each case up to 6 carbon atoms,

[0012] R5, R8 and R9 are identical or different and denote hydrogen, cycloalkyl having 3 to 6 carbon atoms, phenyl, straight-chain or branched alkoxycarbonyl having up to 6 carbon atoms or straight-chain or branched alkyl having up to 6 carbon atoms, which is optionally substituted by halogen,

[0013] or, in the case where T represents a nitrogen atom, R5 can also denote benzyl,

[0014] E and L are identical or different and represent hydrogen, halogen, trifluoromethyl, hydroxyl or carboxyl, or represent straight-chain or branched alkyl, alkoxy or alkoxycarbonyl having in each case up to 6 carbon atoms,

[0015] R1 represents cycloalkyl having 3 to 10 carbon atoms, or represents straight-chain or branched alkyl having 1 to 10 carbon atoms, or represents phenyl, which is optionally substituted up to twice in an identical or different manner by halogen, cyano, hydroxyl or straight-chain or branched alkyl or alkoxy having in each case up to 4 carbon atoms,

[0016] R2 represents hydrogen or straight-chain or branched alkyl having up to 3 carbon atoms,

[0017] R3 represents hydrogen or straight-chain or branched alkyl having up to 5 carbon atoms, or represents cycloalkyl having 3 to 7 carbon atoms, or represents phenyl, or represents a 5- to 7-membered aromatic heterocyclic radical having up to 3 heteroatoms from the series consisting of S, N and/or O, each of which is optionally substituted up to 3 times in an identical or different manner by halogen, nitro, phenyl, hydroxyl or by straight-chain or branched alkyl or alkoxy having up to 6 carbon atoms,

[0018] R4 represents hydrogen, or represents a group of the formula —CH2—OH or CH2O—CO—R12,

[0019] wherein

[0020] R12 denotes hydrogen, straight-chain or branched alkyl having up to 8 carbon atoms or phenyl, which is optionally substituted up to 3 times in an identical or different manner by halogen, hydroxyl, cyano or straight-chain or branched alkyl or alkoxy having in each case up to 4 carbon atoms,

[0021] and salts thereof.

[0022] The phenylacetic acid derivatives with heterocyclic substituents and substituted imidazo[4,5-b]pyridines and benzimidazoles according to the invention can also be in the form of their salts. Salts with organic or inorganic bases or acids may be mentioned in general here.

[0023] Physiologically acceptable salts are preferred in the context of the present invention. Physiologically acceptable salts of the compounds according to the invention can be salts of the substances according to the invention with mineral acids, carboxylic acids or sulphonic acids. Particularly preferred salts are, for example, those with hydrochloric acid, hydrobromic acid, sulphuric acid, phosphoric acid, methanesulphonic acid, ethanesulphonic acid, toluenesulphonic acid, benzenesulphonic acid, naphthalenedisulphonic acid, acetic acid, propionic acid, lactic acid, tartaric acid, citric acid, fumaric acid, maleic acid or benzoic acid.

[0024] Physiologically acceptable salts can likewise be metal or ammonium salts of the compounds according to the invention which have a free carboxyl group. Particularly preferred salts are, for example, sodium, potassium, magnesium or calcium salts, as well as ammonium salts which are derived from ammonia or organic amines, such as, for example, ethylamine, di- or triethylamine, di- or triethanolamine, dicyclohexylamine, dimethylaminoethanol, arginine, lysine, ethylenediamine or 2-phenylethylamine.

[0025] The compounds according to the invention can exist in stereoisomeric forms which either behave as mirror images (enantiomers) or do not behave as mirror images (diastereomers). The invention relates both to the enantiomers or diastereomers and to the particular mixtures thereof. These mixtures of the enantiomers and diastereomers can be separated into the stereoisomerically uniform constituents in a known manner.

[0026] In the context of the invention, a heterocyclic radical, which is optionally benzo-fused, in general represents a saturated or unsaturated 5- to 7-membered, preferably 5- or 6-membered, heterocyclic radical which can contain up to 3 heteroatoms from the series consisting of S, N and/or O and which, in the case of a nitrogen atom, can also be bonded via this. Examples which may be mentioned are: indolyl, quinolyl, benzo[b]thienyl, benzo[b]furyl, pyridyl, thienyl, furyl, pyrrolyl, thiazolyl, oxazolyl, imidazolyl, morpholinyl or piperidyl. Quinolyl, furyl, pyridyl and thienyl are preferred.

[0027] Preferred compounds of the general formula (I) are those in which

[0028] D represents a radical of the formula 3

[0029] wherein

[0030] T denotes a nitrogen atom or the —CH group,

[0031] R6, R7, R10 and R11 are identical or different and denote hydrogen, trifluoromethyl, fluorine, chlorine, bromine or straight-chain or branched alkyl or alkoxy having in each case up to 5 carbon atoms,

[0032] R5, R8 and R9 are identical or different and denote hydrogen, cyclopropyl, cyclopentyl, cyclohexyl, phenyl, straight-chain or branched alkoxycarbonyl having up to 5 carbon atoms or straight-chain or branched alkyl having up to 5 carbon atoms, which is optionally substituted by fluorine, chlorine or bromine,

[0033] or, in the case where T represents a nitrogen atom, R5 can also denote benzyl,

[0034] E and L are identical or different and represent hydrogen, fluorine, chlorine, bromine, trifluoromethyl, hydroxyl or straight-chain or branched alkyl or alkoxy having in each case up to 4 carbon atoms,

[0035] R1 represents cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl, or represents straight-chain or branched alkyl having up to 7 carbon atoms, or represents phenyl, which is optionally substituted by fluorine, chlorine, bromine, cyano, hydroxyl or straight-chain or branched alkyl or alkoxy having in each case up to 3 carbon atoms,

[0036] R2 denotes hydrogen or methyl,

[0037] R3 represents hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms, cyclopropyl, cyclopentyl or cyclohexyl, or represents phenyl, pyridyl, thienyl or furyl, each of which is optionally substituted up to twice in an identical or different manner by fluorine, chlorine, bromine, phenyl, nitro, hydroxyl or by straight-chain or branched alkyl or alkoxy having up to 4 carbon atoms,

[0038] R4 represents hydrogen, or represents a group of the formula —CH2—OH or —CH2O—CO—R12,

[0039] wherein

[0040] R12 denotes hydrogen, straight-chain or branched alkyl having up to 6 carbon atoms or phenyl, which is optionally substituted up to twice in an identical or different manner by fluorine, chlorine, bromine, cyano, hydroxyl or straight-chain or branched alkyl or alkoxy having in each case up to 3 carbon atoms,

[0041] and salts thereof.

[0042] Particularly preferred compounds of the general formula (I) are those in which

[0043] D represents a radical of the formula 4

[0044] wherein

[0045] T denotes a nitrogen atom or the —CH group,

[0046] R6, R7, R10 and R11 are identical or different and denote hydrogen, trifluoromethyl, chlorine or straight-chain or branched alkyl or alkoxy having in each case up to 4 carbon atoms,

[0047] R5, R8 and R9 are identical or different and denote hydrogen, cyclopropyl, cyclopentyl, cyclohexyl, phenyl straight-chain or branched alkoxycarbonyl having up to 4 carbon atoms or straight-chain or branched alkyl having up to 4 carbon atoms, which is optionally substituted by chlorine,

[0048] or, in the case where T represents a nitrogen atom, R5 can also denote benzyl,

[0049] E and L are identical or different and represent hydrogen, fluorine, chlorine, bromine or trifluoromethyl,

[0050] R1 represents cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl, or represents straight-chain or branched alkyl having up to 6 carbon atoms, or represents phenyl, which is optionally substituted by fluorine, chlorine, cyano, hydroxyl, methyl or methoxy,

[0051] R2 represents hydrogen or methyl,

[0052] R3 represents hydrogen or straight-chain or branched alkyl having up to 3 carbon atoms, cyclopropyl, cyclopentyl or cyclohexyl, or represents pyridyl, thienyl or phenyl, each of which is optionally substituted up to twice in an identical or different manner by fluorine, chlorine, bromine, nitro, phenyl, hydroxyl or by straight-chain or branched alkyl or alkoxy having up to 3 carbon atoms,

[0053] R4 represents hydrogen, or represents a group of the formula —CH2—OH or —CH2O—CO—R12,

[0054] wherein

[0055] R2 denotes hydrogen, straight-chain or branched alkyl having up to 6 carbon atoms or phenyl, which is optionally substituted up to twice in an identical or different manner by fluorine, chlorine, bromine, cyano, hydroxyl, methyl or methoxy,

[0056] and salts thereof.

[0057] A process has furthermore been found for the preparation of the compounds of the general formula (I) according to the invention, characterized in that compounds of the general formula (II) 5

[0058] in which

[0059] D, E, L and R1 have the abovementioned meaning, are reacted with compounds of the general formula (III) 6

[0060]  in which

[0061] R2, R3 and R4 have the abovementioned meaning, in inert solvents, if appropriate in the presence of a base and/or auxiliary.

[0062] The process according to the invention can be illustrated by the following equation by way of example: 7

[0063] Suitable solvents for the process are customary organic solvents which do not change under the reaction conditions. These include, preferably, ethers, such as diethyl ether, dioxane, tetrahydrofuran or glycol dimethyl ether, or hydrocarbons, such as benzene, toluene, xylene, hexane, cyclohexane or petroleum fractions, or halogenated hydrocarbons, such as methylene chloride, chloroform, carbon tetrachloride, dichloroethylene, trichloroethylene or chlorobenzene, or ethyl acetate, triethylamine, pyridine, dimethyl sulphoxide, dimethylformamide, hexamethylphosphoric acid triamide, acetonitrile, acetone or nitromethane. It is also possible to use mixtures of the solvents mentioned. Methylene chloride; tetrahydrofuran and dimethylformamide are preferred.

[0064] Bases which can be employed for the process according to the invention are in general inorganic or organic bases. These include, preferably, alkali metal hydroxides, such as, for example, sodium hydroxide or potassium hydroxide, alkaline earth metal hydroxides, such as, for example, barium hydroxide, alkali metal carbonates, such as sodium carbonate, potassium carbonate or caesium carbonate, alkaline earth metal carbonates, such as calcium carbonate, or alkali metal or alkaline earth metal alcoholates, such as sodium or potassium methanolate, sodium or potassium ethanolate or potassium tert-butylate, or organic amines (trialkyl(C1-C6)amines), such as triethylamine, or heterocyclic compounds, such as 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), pyridine, diaminopyridine, methylpiperidine or morpholine. It is also possible to employ alkali metals, such as sodium, or hydrides thereof, such as sodium hydride, as bases. Sodium hydride, potassium carbonate, triethylamine, trimethylamine, pyridine, potassium tert-butylate, DBU or DABCO are preferred.

[0065] The base is in general employed in an amount of 0.05 mol to 10 mol, preferably 1 mol to 2 mol, per mole of compound of the formula (II).

[0066] If appropriate, the amidation can proceed by the activated stage of the acid halides or mixed anhydrides, which can be prepared from the corresponding acids by reaction with thionyl chloride, phosphorus trichloride, phosphorus pentachloride, phosphorus tribromide or oxalyl chloride or methanesulphonyl chloride.

[0067] Suitable dehydrating reagents are carbodiimides, such as, for example, diisopropylcarbodiimide, dicyclohexylcarbodiimide or N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, or carbonyl compounds, such as carbonyldiimidazole, or 1,2-oxazolium compounds, such as 2-ethyl-5-phenyl-1,2-oxazolium 3-sulphonate, or propanephosphoric anhydride or isobutyl chloroformate or benzotriazolyloxy-tris-(dimethylamino)phosphonium hexafluorophosphate or phosphonic acid diphenyl ester-amide or methanesulphonyl chloride, if appropriate in the presence of bases, such as triethylamine or N-ethylmorpholine or N-methylpiperidine or dicyclohexylcarbodiimide and N-hydroxysuccinimide.

[0068] The process according to the invention is in general carried out in a temperature range from −50° C. to +100° C., preferably from −30° C. to +60° C.

[0069] The process according to the invention is in general carried out under normal pressure. However, it is also possible to carry out the process under increased pressure or under reduced pressure (for example in a range from 0.5 to 5 bar).

[0070] The compounds of the general formula (II) are known in some cases or are novel, and can then be prepared, for example, by reaction of compounds of the general formula (IV)

D-H  (IV)

[0071] in which

[0072] D has the abovementioned meaning, with compounds of the general formula (V) 8

[0073] in which

[0074] E, L and R1 have the abovementioned meaning,

[0075] X stands for halogen and

[0076] Y represents straight-chain or branched alkyl having up to 4 carbon atoms, in one of the abovementioned solvents and bases, and finally conversion of the esters into the corresponding acids by customary methods.

[0077] Dimethylformamide is preferably employed as the solvent.

[0078] Sodium hydride is preferably employed as the base.

[0079] The base is in general employed in an amount of 0.05 mol to 10 mol, preferably 1 mol to 2 mol, per mole of the compound of the formula (IV).

[0080] The process according to the invention is in general carried out in a temperature range from −50° C. to +100° C., preferably from −30° C. to +60° C.

[0081] The process according to the invention is in general carried out under normal pressure. However, it is also possible to carry out the process under increased pressure or under reduced pressure (for example in a range from 0.5 to 5 bar).

[0082] The compounds of the general formula (III), (IV) and (V) are known per se or can be prepared by customary methods.

[0083] The compounds of the general formula (I) according to the invention have a pharmacological action spectrum which cannot be foreseen.

[0084] They can be used as active compounds in medicaments for reducing changes to vascular walls and for treatment of coronary heart diseases, cardiac insufficiency, disturbances in cerebral performance, ischaemic cerebral diseases, apoplexy, circulatory disturbances, disturbances in microcirculation and thromboses.

[0085] The proliferation of smooth muscle cells continues to play a decisive role in the occlusion of vessels. The compounds according to the invention are suitable for inhibiting this proliferation and therefore for preventing atherosclerotic processes.

[0086] The compounds according to the invention are distinguished by a reduction in ApoB-100-associated lipoproteins (VLDL and its breakdown products, such as, for example, LDL), ApoB-100, triglycerides and cholesterol. They therefore have valuable pharmacological properties which are superior to the prior art.

[0087] Surprisingly, the action of the compounds according to the invention initially comprises a reduction in or complete inhibition of the formation and/or release of ApoB-100-associated lipoproteins from hepatic cells, which results in a lowering of the plasma VLDL level. This lowering in VLDL must be accompanied by a lowering of the plasma level of ApoB-100, LDL, triglycerides and cholesterol; several of the abovementioned risk factors which participate in changes to the vascular wall are thus reduced simultaneously.

[0088] The compounds according to the invention can therefore be employed for prevention and treatment of atherosclerosis, obesity, pancreatitis and constipation.

[0089] 1. Inhibition of the release of ApoB-100-associated lipoproteins

[0090] The test for detection of the inhibition of the release of ApoB-100-associated lipoproteins from hepatic cells was carried out in vitro with cultured hepatic cells, preferably with cells of the human line HepG2. These cells are cultured under standard conditions in medium for culture of eukaryotic cells, preferably in RPM 1640 with 10% foetal calf serum. HepG2 cells synthesize and secrete into the culture supernatant ApoB-100-associated lipoprotein particles, which are in principle built up similarly to the VLDL and LDL particles which are to be found in plasma.

[0091] These particles can be detected with an immunoassay for human LDL. This immunoassay is carried out with antibodies which have been induced against human LDL in rabbits under standard conditions. The anti-LDL antibodies (rab-anti-LDL-Ab) were purified by affinity chromatography on an immunosorbent with human LDL. These purified rab-anti-LDL-Ab are adsorbed onto the surface of plastic. This adsorption is expediently carried out on the plastic surface of microtiter plates with 96 wells, preferably on MaxiSorp plates. If ApoB-100-associated particles are present in the supernatant of Hep-G2 cells, these can then bond to the insolubilized rab-anti-LDL-Ab, and an immune complex bonded to the plastic surface is formed. Non-bonded proteins are removed by washing. The immune complex on the plastic surface is detected with monoclonal antibodies, which have been induced against human LDL and purified under standard conditions. These antibodies were conjugated with the enzyme peroxidase. Peroxidase converts the colourless substrate TMB into a coloured product in the presence of H2O2. After acidification of the reaction mixture with H2SO4, the specific adsorption of light is determined at 450 nm, this being a measure of the amount of ApoB-100-associated particles which had been secreted by the HepG2 cells into the culture supernatant.

[0092] Surprisingly, the compounds according to the invention inhibit the release of ApoB-100-associated particles. The IC50 value indicates the concentration of substance at which the adsorption of light is inhibited by 50% compared with the control (solvent control without the substance).

Example No. IC50 [10−9 mol/l]
2           29.5
3           62.5
4           40.5
5           83.2
6           37.4
7           228.9
8           50.5
11          489.2
12          10.8
13          24.6
14          473.5
15          47.3
16          35.9
17          38.1
20          8.1
21          4.5
22          23.5
23          36.1
26          26.8
27          6.5
28          62.6
29          40.5
30          128.5
31          428.3
32          48.5
33          41.1
34          12.3
35          308.0
36          72.5
37          31.1
38          269.7
39          29.4
40          93.6
41          40.4
42          9.3
43          5.0
44          130.6
45          302.4
46          58.9
47          78.6
48          491.2
49          78.6
50          420.6
51          78.6
52          58.9
53          491.2
54          56.7
55          18.9
56          170.1
57          10.8
58          1.5
59          26.9
60          9.5
62          151.9
63          1010.1
64          150.9
66          8.1
67          30.2
69          9.6

[0093] 2. Determination of VLDL secretion in vivo on the hamster

[0094] The effect of the test substances on VLDL secretion in vivo is investigated on the hamster. For this, after premedication with atropine (83 mg/kg s.c.), golden hamsters are anaesthetized with Ketavet (83 mg/kg s.c.) and nembutal (50 mg/kg i.p.). When the animals had become reflex-free, the v.jugularis is exposed and cannulated. 0.25 ml/kg of a 20% strength solution of Triton WR-1339 in physiological saline solution is then administered. This detergent inhibits lipoprotein lipase and thus leads to an increase in the triglyceride level because of an absence of catabolism of secreted VLDL particles. This increase in triglycerides can be used as a measure for the rate of VLDL secretion. Blood was taken from the animals by puncture of the retroorbital venous plexus before and one and two hours after administration of the detergent. The blood is incubated at room temperature for two hours and then at 4° C. overnight in order to conclude coagulation completely. Thereafter, it is centrifuged at 10,000 g for 5 minutes. The triglyceride concentration in the serum thus obtained is determined with the aid of a modified commercially obtainable enzyme test (Merckotest® Triglyceride No. 14354). 100 μl of test reagent are added to 100 μl of serum in 96-well plates and the plates are incubated at room temperature for 10 minutes. The optical density is then determined at a wavelength of 492 nm in an automatic plate reader (SLT-Spectra). Serum samples having a triglyceride concentration which is too high are diluted with physiological saline solution. The triglyceride concentration contained in the samples is determined with the aid of a standard curve measured in parallel. In this model, test substances are administered either intravenously immediately before administration of the detergent or orally or subcutaneously before initiation of the anaesthesia.

[0095] 3. Inhibition of intestinal triglyceride absorption in vivo (rats)

[0096] The substances which are to be investigated in vivo for their inhibiting action on triglyceride absorption are administered orally to male Wistar rats having a body weight of between 170 and 230 g. For this purpose, the animals are divided into groups of 6 animals 18 hours before administration of the substance, and their food is then withdrawn. Drinking water is available to the animals ad libitum. The animals of the control groups are given an aqueous tragacanth suspension or a tragacanth suspension which comprises olive oil. The tragacanth-olive oil suspension is prepared with an Ultra-Turrax. The substances to be investigated are suspended in a corresponding tragacanth-olive oil suspension, likewise with an Ultra-Turrax, directly before administration of the substance.

[0097] Before administration by a stomach tube, blood is taken from each rat by puncture of the retroorbital venous plexus for determination of the basal serum triglyceride content. The tragacanth suspension, the tragacanth-olive oil suspensions without a substance (control animals) or the substances, suspended in a corresponding tragacanth-olive oil suspension, are then administered to the fasting animals using a stomach tube. Further blood for determination of the postprandial serum triglyceride increase is as a rule taken 1, 2 and 3 hours after the administration by a stomach tube.

[0098] The blood samples are centrifuged and, after isolation of the serum, the triglycerides are determined photometrically with an EPOS-Analyzer 5060 (Eppendorf Gerätebau, Netheler & Hinz GmbH, Hamburg). The triglycerides are determined completely enzymatically with a commercially available UV test.

[0099] The postprandial increase in serum triglycerides is determined by subtraction of the triglyceride prevalue of each animal from its corresponding postprandial triglyceride concentrations (1, 2 and 3 hours after administration).

[0100] The average is taken of the differences (in mmol/l) at each point in time (1, 2 and 3 hours) in the groups, and the means of the increase in serum triglycerides (ΔTG) of the animals treated with the substance are compared with the animals which received only the tragacanth-oil suspension.

[0101] The course of the serum triglycerides in the control animals, which were given only tragacanth, is also calculated. The effect of the substance at each point in time (1, 2 or 3 hours) is determined as follows and stated in Δ% of the oil-loaded control. 1$\Delta \%\mathrm{increase}\mathrm{in}\mathrm{triglyceride}=\frac{\Delta {\mathrm{TG}}_{\mathrm{substance}}-\Delta {\mathrm{TG}}_{\mathrm{tragacanth}\mathrm{control}}}{\Delta {\mathrm{TG}}_{\mathrm{oil}\mathrm{loading}}-\Delta {\mathrm{TG}}_{\mathrm{tragacanth}\mathrm{control}}}\times 100$

[0102] Effect of 10 mg of test substance/kg of body weight p.o. on the increase in triglycerides (Δ%) 2 hours after a triglyceride loading in the serum of fasting rats. The increase in serum triglycerides of fat-loaded control animals, based on the serum triglycerides level of tragacanth control animals, corresponds to 100%. n=6 animals per group.

[0103] The statistical analysis is carried out with the Student t-test after first checking the variances for homogeneity.

[0104] Substances which statistically significantly (p<0.05) reduce the postprandial increase in serum triglycerides by at least 30%, compared with the untreated control group, at a point in time are regarded as pharmacologically active.

		TG absorption ED50
		or % inhibition
	Example No.	mg/kg/p.o.
	2		<3	mg/kg
	4		<10	mg/kg
	12		<<3	mg/kg
	13		<3	mg/kg
	16		>2	mg/kg
	20		>>10	mg/kg
	21		>>6	mg/kg
	23		2	mg/kg
	27		>2	mg/kg
	29		3	mg/kg
	34		2	mg/kg
	37		>2	mg/kg
	41		10	mg/kg
	42		>2	mg/kg
	43		10	mg/kg
	46		30	mg/kg
	51		40	mg/kg
	54		50	mg/kg
	57		>3	mg/kg
	60		10	mg/kg
	67		>3	mg/kg

[0105] 4. Inhibition of VLDL secretion in vivo (rat)

[0106] The action of the test substances on VLDL secretion is also investigated on the rat. For this, Triton WR-1339, dissolved in physiological saline solution, is administered intravenously into the tail vein of rats of 500 mg/kg body weight (2.5 mg/kg). Triton WR-1339 inhibits lipoprotein lipase and thus leads to an increase in the triglyceride and cholesterol level due to inhibition of VLDL catabolism. These increases can be used as a measure of the rate of VLDL secretion.

[0107] Blood is taken from the animals by puncture of the retroorbital venous plexus before and one and two hours after administration of the detergent. The blood is incubated at room temperature for 1 hour, for coagulation, and the serum is isolated by centrifugation at 10,000 g for 20 seconds. The triglycerides are then determined photometrically at a wavelength of 540 nm by means of a commercially available coupled enzyme test (Sigma Diagnostics®, No. 339). Measurement is carried out with the aid of a similarly coupled enzyme test (Boehringer Mannheim®, No. 1442350) at a wavelength of 546 nm. Samples with triglyceride or cholesterol concentrations which exceed the measurement range of the methods are diluted with physiological saline solution. The particular serum concentrations are determined with the aid of standard series measured in parallel. Test substances are administered orally, intravenously or subcutaneously immediately after the Triton injection.

[0108] The invention furthermore relates to the combination of phenylacetic acid derivatives with heterocyclic substituents and substituted imidazo[4.5-b]pyridines and berzimidazoles of the general formula (I) with a glucosidase and/or amylase inhibitor for treatment of familial hyperlipidaemias, obesity (adiposity) and diabetes mellitus. Glucosidase and/or amylase inhibitors in the context of the invention are, for example, acarbose, adiposine, voglibose (AO-128), miglitol, emiglitate, MDL-25637, camiglibase (MDL-73945), temdamistate, AI-3688, trestatin, pradimicin-Q and salbostatin. The combination of acarbose, miglitol, emiglitate or vaglibose with one of the abovementioned compounds of the general formula (I) according to the invention is preferred.

[0109] The new active compounds can be converted in a known manner into the customary formulations, such as tablets, coated tablets, pills, granules, aerosols, syrups, emulsions, suspensions and solutions, using inert, non-toxic, pharmaceutically suitable excipients or solvents. The therapeutically active compound should be present here in each case in a concentration of about 0.5 to 90% by weight of the total mixture, i.e. in amounts which are sufficient to achieve the stated dosage range.

[0110] The formulations are prepared, for example, by extending the active compounds with solvents and/or excipients, if appropriate using emulsifying agents and/or dispersing agents, it being possible, for example in the case where water is used as the diluent, to use organic solvents as auxiliary solvents if appropriate.

[0111] The formulations are administered in the customary manner, preferably orally or parenterally, in particular perlingually or intravenously.

[0112] In the case of parenteral use, solutions of the active compound can be employed, using suitable liquid carrier materials.

[0113] In general, it has proved advantageous in the case of intravenous administration to administer amounts of about 0.001 to 1 mg/kg, preferably about 0.01 to 0.5 mg/kg of body weight to achieve effective results, and in the case of oral administration the dosage is about 0.01 to 20 mg/kg, preferably 0.1 to 10 mg/kg of body weight.

[0114] Nevertheless, it may be necessary, if appropriate, to deviate from the amounts mentioned, in particular as a function of the body weight or the nature of the administration route, of the behaviour of the individual towards the medicament, the nature of the formulation thereof and the time or interval at which administration takes place. Thus, in some cases it may be sufficient to manage with less than the abovementioned minimum amount, while in other cases the upper limit mentioned must be exceeded. In the case where relatively large amounts are administered, it may be advisable to divide these into several individual doses over the course of the day.

[0115] Abbreviations used:

[0116] bs=Broad singlet

[0117] CI=Chemical Ionization

[0118] cHept=Cycloheptyl

[0119] cHex=Cyclohexyl

[0120] cPent=Cyclopentyl

[0121] DCCI=N′-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide

[0122] d=Doublet

[0123] dia=Diastereomer

[0124] dd=Doublet doublets

[0125] DMF=N,N-Dimethylformamide

[0126] DMSO=Dimethyl sulphoxide

[0127] EI=Electron impact ionization

[0128] FAB=Fast Atom Bombardment

[0129] HOBT=1-Hydroxy-1H-benzotriazole

[0130] Hz=Hertz

[0131] iBu=Isobutyl

[0132] iPr=Isopropyl

[0133] m=Multiplet

[0134] Me=Methyl

[0135] nPr=Normal propyl

[0136] Ph=Phenyl

[0137] RT=Room temperature

[0138] s=Singlet

[0139] t=Triplet

[0140] TFA=Trifluoroacetic acid

[0141] THF=Tetrahydrofuran

[0142] TMS=Tetramethylsilane

[0143] Solvent mixtures used

[0144] Petroleum ether:acetone=1:1 (A)

[0145] Petroleum ether:ethyl acetate=20:1 (B)

[0146] Petroleum ether:ethyl acetate=10:1 (C)

[0147] Petroleum ether:ethyl acetate=5:1 (D)

[0148] Petroleum ether:ethyl acetate=3:1 (E)

[0149] Petroleum ether:ethyl acetate=4:1 (F)

[0150] Petroleum ether:ethyl acetate=2:1 (G)

[0151] Petroleum ether:ethyl acetate=1:1 (H)

[0152] Petroleum ether:ethyl acetate=1:2 (I)

[0153] Methylene chloride:methanol=50:1 (J)

[0154] Methylene chloride:methanol=20:1 (K)

[0155] Methylene chloride:methanol=10:1 (L)

[0156] Methylene chloride:ethyl acetate=1:1 (M)

[0157] Methylene chloride:ethanol=50:1 (N)

[0158] Methylene chloride (100%)=(O)

[0159] Ethyl acetate:methanol=10:1 (P)

[0160] Toluene (100%)=(Q)

[0161] Toluene:ethyl acetate=1:1 (R)

[0162] Toluene:ethyl acetate=8:1 (S)

[0163] Toluene:ethyl acetate=9:1 (T)

[0164] Cyclohexanol:ethyl acetate=1:1 (U)

[0165] Cyclohexanol:ethyl acetate=7:3 (V)

[0166] Additional information:

[0167] If FAB has not been used, the following identifications apply in all the Tables which follow:

[0168] *=EI

[0169] #=CI (NH3)

EXAMPLE I

[0170] Methyl 2-(R&S)-phenyl-2-(4-methyl)phenylacetate 9

[0171] 21.0 g (100 mmol, apin) of 2-phenyl-1-(4-methyl)phenyl-1-oxoethane and 38.8 g (120 mmol) of iodobenzene diacetate were dissolved in 300 ml of trimethyl orthoformate. 19.6 g of concentrated sulphuric acid were added to this solution and the solution was heated at 60° C. for 6 hours. It was cooled to room temperature, diluted with water (100 ml) and extracted with diethyl ether. The combined organic phases were dried over sodium sulphate and concentrated on a rotary evaporator. The residue was purified by column chromatography.

[0172] Yield 13.1 g (55%); Rf=0.33 (petroleum ether:ethyl acetate=20:1); Mass (calculated) for C16H16O2=240.30; mass spectrum (FAB, relative intensity) 241 (25%), 181 (100%); 1H NMR (200 MHz, CDCl3) δ 7.3-7.10 (m, 9 H), 4.99 (s, 1 H), 3.73 (s, 3 H), 2.31 (s, 3 H).

EXAMPLE II

[0173] tert-Butyl 2-cyclopentyl-2-(4-methylphenyl)-acetate 10

[0174] 33.5 g (0.3 mol) of potassium tert-butylate are initially introduced into 100 ml of anhydrous DMF at 0° C., and 51.6 g (0.25 mol) of tert-butyl 4-methylphenyl-acetate in 250 ml of anhydrous DMF are added dropwise. The mixture is stirred at 0° C. for 30 minutes, 32.2 ml (0.3 mol) of cyclopentyl bromide in 150 ml of anhydrous DMF are added dropwise at 5-15° C. and the mixture is stirred at 25° C. for 20 hours. After concentration, the residue is partitioned between water and diethyl ether and the ether phase is dried over sodium sulphate and concentrated. The product crystallizes out.

[0175] Yield. 67 g (97.5% of theory); Melting point: 51-53° C.

[0176] The compounds of Table I are prepared analogously to the instructions of Example II:

[0177] Table I: 11

	Example No.	R1	R18	Rf*
	III	(R&S) iPr	Me	0.86 (T)
	IV	(R&S) iBu	tBu	0.84 (S)
	V	(R&S) cPent	Mc	0.59 (C)
	VI	(R&S) cHex	Me	0.38 (B)
	VII	(R&S) cHex	tBu	0.71 (Q)
	VIII	(R&S) cHept	Me	0.57 (Q)
	IX	(R&S) cHept	tBu	0.32 (Q)

EXAMPLE X

[0178] tert-Butyl 2-(4-bromomethyl-phenyl)-2-cyclopentyl-acetate 12

[0179] 27.4 g (0.1 mol) of the compound from Example II are dissolved in 200 ml of carbon tetrachloride and the solution is heated to the boiling point After addition of 0.82 g of azobisisobutyronitrile, 18.7 g (0.105 mol) of N-bromosuccinimide are added in portions, the mixture is then refluxed for 1 hour and cooled to 0° C. and the succinimide is filtered off. After concentration of the filtrate, the product precipitates out. It is washed with petroleum ether (40/60) and dried.

[0180] Yield: 20 g (57% of theory); Melting point: 73-76° C.

[0181] The compounds of Table II are prepared analogously to the instructions of Example X:

TABLE II
13
	Example No.	R1	R13	Rf*
	XI	(R & S) iPr	Me	0.78 (O)
	XII	(R & S) iBu	tBu	0.86 (O)
	XIII	(R & S) cPent	Me	0.63 (O)
	XIV	(R & S) cHex	Me	0.74 (O)
	XV	(R & S) cHex	tBu	0.58 (O)
	XVI	(R & S) cHept	tBu	0.84 (O)
	XVII	(R & S) Ph	Me	0.74 (O)

EXAMPLE XVIII

[0182] 4-Methyl-2-propyl-1H-benzimidazole 14

[0183] 5.00 g (41 mmol) of 2,3-diamino-toluene and 3.7 ml (41 mmol) of butyric acid are stirred in 41 ml of polyphosphoric acid at 120° C. for 3 hours, and the mixture is poured into 410 ml of water and brought to pH=9 with solid sodium hydride. Thereafter, sodium carbonate is added until the evolution of carbon dioxide has ended, the mixture is extracted five times with 100 ml of ethyl acetate each time, the combined organic phases are dried with anhydrous sodium sulphate and the solution is evaporated - finally under a high vacuum; crude weight: 5.89 g. The product is purified by chromatography over silica gel 60 (Merck/40 - 63 μm/methylene chloride:methanol=100:1);

[0184] yield: 2.45 (34%). Rf=0.16 (methylene chloride:methanol=20:1)

[0185] The compounds listed in Table III are prepared analogously to the instructions of Example XVIII:

		Yield		Melting	MS (EI)
Example No.	D	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
XIX	15	97	0.85 (P)	>240	200 (30%), 145 (100%)
XX	16	81	0.66 (P)	>220	199 (100%)
XXI	17	27	0.39 (K)	184	238 (100%)
XXII	18	23	0.39 (K)	240	#224 (100%)

EXAMPLE XXIII

[0186] tert-Butyl 2-cycloheptyl-2-[4-(2,3-dimethylindol-1-ylmethyl)phenyl]acetate 19

[0187] 1.0 g (6.2 mmol) of 2,3-dimethylindole is dissolved in 10 ml of DMF. The solution is cooled to 0° C. under argon. 0.32 g (8.0 mmol) of sodium hydride (60% pure in paraffin) is added in portions. The mixture is subsequently stirred at 0° C. for half an hour. A solution of 3.5 g (6.2 mmol) of XVI in 40 ml of DMF is slowly added dropwise. The mixture is subsequently stirred at room temperature overnight. The DMF is distilled off using a high vacuum and the residue is partitioned in water and ethyl acetate. The organic phase is dried over sodium sulphate and concentrated on a rotary evaporator. The residue is purified by column chromatography (silica gel Merck 60 (0.040-0.063)):

[0188] Yield: 1.3 g (42.3%); Melting point=121-123° C.; Rf=0.42 (petroleum ether:ethyl acetate=20:1); Mass (calculated) for C30H39NO2=445.65: mass spectrum (DCI (NH3), relative intensity) 445 (100%), 389 (30%); 1H NMR (250 MHz, CDCl3) δ 7.52 (m, 1 H), 7.22-7.18 (m, 3 H), 7.15-7.05 (m, 2 H), 6.89 (d, J=8.22 Hz, 2 H), 5.25 (s, 2 H.), 3.12 (d, J=10.83 Hz, 1 H), 2.28 (s, 3 H), 2.27 (s, 3 D, 2.12 (m, 1 H), 1.84-1.20 (m, 11 H), 1.36 (s, 9 H), 0.90 (m, 1 H).

[0189] The compounds listed in Table IV are prepared analogously to the instructions of Example XXIII:

TABLE IV
20
				Yield		Melting	MS (*)
Example No.	D	R14	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
XXIV	21	tBu	(R & S) cHept	48	0.43 (B)	123-124	#418 (100%)
XXV	22	tBu	(R & S) cHept	49	0.43 (B)	124-26	*431 (100%), 57 (70%)
XXVI	23	tBu	(R & S) cPent	24	0.25 (B)	115	417 (100%), 360 (20%)
XXVI	24	Me	(R & S) cHex	55	0.35 (K)	98-101	389 (100%)

[0190]

				Yield		Melting	MS (*)
Example No.	D	R14	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
XXVIII	25	tBu	(R & S) cHept	39	0.39 (B)		*493 (20%),   57 (100%)
XXIX	26	tBu	(R & S) cPent	48	0.46 (C)	86	461 (85%), 406 (100%)
XXX	27	tBu	(R & S) cHept	43	0.32 (B)		*489 (60%),  (100%)
XXXI	28	tBu	(R & S) cPent	42	0.58 (O)	106	491 (100%), 436 (40%), 57 (60%)
XXXII	29	tBu	(R & S) cPent	64	0.25 (L)	100-02	495 (70%), 440 (100%),  57 (100%)
XXXIII	30	tBu	(R & S) cHept	76	0.41 (B)	100-01	#431 (20%),  375 (100%)
XXXIV	31	tBu	(R & S) cPent	65	0.42 (B)		403 (20%), 346 (40%)

[0191]

				Yield		Melting	MS (*)
Example No.	D	R14	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
XXXV	32	tBu	(R & S) cPent	67	0.33 (B)		#404 (100%)
XXXVI	33	tBu	(R & S) cHept	54	0.35 (B)		*451 (30%),   57 (100%)
XXXVII	34	tBu	(R & S) cPent	75	0.34 (B)		423 (100%)
XXXVIII	35	tBu	(R & S) cPent	79	0.25 (B)		#420 (100%)
XXXIX	36	tBu	(R & S) cHept	53	0.24 (B)		*447 (100%), 57 (60%)

[0192]

				Yield		Melting	MS (*)
Example No.	D	R14	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
XL	37	tBu	(R & S) cHept	67	0.14 (J)		*418 (10%),   57 (100%)
XLI	38	tBu	(R & S) cHept	96	0.42 (K)	109	*432 (100%)
XLII	39	tBu	(R & S) cPent	53	0.20 (E)		475 (80%), 473 (100%)
XLIII	40	tBu	(R & S) cPent	70	0.48 (J)	111
XLIV	41	tBu	(R & S) cPent	94	0.81 (K)
XLV	42	tBu	(R & S) cPent	55	0.64 (J)	134	447 (100%)
XLVI	43	tBu	(R & S) cPent	66	0.47 (K)	56	473 (100%), 57 (40%)

[0193]

				Yield		Melting	MS (*)
Example No.	D	R14	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
XLVII	44	tBu	(R & S) cPent	80	0.51 (G)
XLVIII	45	tBu	(R & S) cPent	61	0.27 (H)
XLIX	46	tBu	(R & S) cHept	20	0.28 (V)	0:1
L	47	tBu	(R & S) cPent	27	0.64 (K)		432 (40%), 57 (100%)
LI	48	tBu	(R & S) cPent	97	0.47 (L)	0:1
LII	49	tBu	(R & S) cPent	32	0.29 (J)	92	434 (100%), 162 (65%)
LIII	50	Me	(R & S) cHex	29	0.59 (K)	0:1	406 (100%)
LIV	51	tBu	(R & S) cPent	43	0.14 (J)	118	460 (100%), 188 (20%)

[0194]

				Yield		Melting	MS (*)
Example No.	D	R14	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
LV	52	tBu	(R & S) cPent	60	0.36 (J)	110	510 (100%), 57 (25%)
LVI	53	tBu	(R & S) cPent	56	0.55 (J) 0.83 (K)	#496 (100%)

EXAMPLES LVII AND LVIII

[0195] 2.30 g (5.0 mmol) of the compound from Example LIV are dissolved in 30 ml of dioxane, and 2.3 ml of concentrated hydrochloric acid are added to the solution. The mixture is boiled to reflux overnight and cooled, and 30 ml of cold water are added. The precipitate formed is filtered off with suction and dried:

[0196] Yield: 1.68 g

[0197] Since, according to NMR and mass, two compounds have been formed, these-were separated by HPLC.

[0198] Cyclopentyl-[4-(2-cyclopropyl-5,7-dimethylimidazo[4,5-b]pyridin-3-yl)-phenyl]-acetic acid 54

[0199] Melting point=180° C.; Rf=0.24 (CH2Cl2:methanol=100:5); Mass (calculated) for C25H29N3O2=403.53: mass spectrum (FAB, relative intensity) 404 (100%).

[0200] {4-[2-(3-Chloropropyl)-5,7-dimethylimidazo[4,5-b]pyridin-3-yl]-phenyl}-cyclopentylacetic acid 55

[0201] Melting point=214° C.; Rf=0.24 (CH2Cl2:methanol=100:5); Mass (calculated) for C25H30ClN3O2=439.99: mass spectrum (FAB, relative intensity) 440 (75%).

EXAMPLE LIX

[0202] 2-Cycloheptyl-2-[4-(2,3-dimethylindol-1-ylmethyl)phenyl]acetic acid 56

[0203] 1.3 g (2.92 mmol) of the compound from Example XXIII are dissolved in 15 ml of dioxane. 1.6 ml of concentrated hydrochloric acid are added and the mixture is boiled to reflux for 6 hours. Water and CH2Cl2 are added and the mixture is extracted. The organic phase is dried over sodium sulphate and concentrated on a rotary evaporator. The residue is purified by column chromatography (silica gel Merck 60 (0.040-0.063)):

[0204] Yield: 1.0 g (88.0%); Rf=0.25 (petroleum ether:ethyl acetate=3:1); Mass (calculated) for C26H31NO2=389.54; mass spectrum (EI, relative intensity) 389 (100%), 149 (45%); 1H NMR (200 MHz, CDCl3) δ 7.51 (m, 1 H), 7.22-7.05 (m, 5 H), 6.89 (d, J=8.11 Hz, 2 H), 5.25 (s, 2 H), 3.20 (d, J=10.88 Hz, 1 H), 2.27 (s, 6 H), 2.18 (m, 1 H), 1.80 (m, 1 H), 1.70-1.17 (m, 10 H), 0.95 (m, 1 H).

TABLE V
57
			Yield		Melting	MS (*)
Example No.	D	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
LX	58	(R & S) cHept	72	0.52 (R)		#362 (100%)
LXI	59	(R & S) cHept	77	0.53 (R)		#376 (100%)

[0205]

			Yield		Melting	MS (*)
Example No.	D	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
LXII	60	(R & S) cPent	80	0.26 (E)	173	361 (100%)
LXIII	61	(R & S) cHex	87	0.51 (K)	209	375 (100%)
LXIV	62	(R & S) cHept	49	0.28 (K)		* 437 (100%), 55 (100%)
LXV	63	(R & S) cPent	72	0.47 (K)	186	406 (80%), 405 (100%)
LXVI	64	(R & S) cHept	68	0.29 (K)		* 433 (100%)

[0206]

			Yield		Melting	MS (*)
Example No.	D	R1	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
LXVII	65	(R & S) cPent	79	0.48 (K)	142	435 (100%)
LXVIlI	66	(R & S) cPent	94	0.48 (K)	67 (Foam)	439 (100%), 217 (80%)
LXIX	67	(R & S) cHept	78	0.27 (E)		*395 (100%)
LXX	68	(R & S) cPent	64	0.44 (K)	oil	*347 (80%),   88 (100%)

[0207]

			Yield		Melting	MS (*) (rela-
Example No.	D	R1	(% of theory)	Rf (solvent)	point (° C.)	tive intensity)
LXXI	69	(R & S) cPent	68	0.62 (K)		*347 (60%),  149 (100%)
LXXII	70	(R & S) cHept	70	0.33 (K)		*395 (80%),  149 (100%)
LXXIII	71	(R & S) cPent	88	0.51 (K)	oil	*367 (100%)

[0208]

			Yield		Melting	MS (*) (rela-
Example No.	D	R1	(% of theory)	Rf (solvent)	point (° C.)	tive intensity)
LXXIV	72	(R & S) cPent	92	0.44 (K)		*363 (100%)
LXXV	73	(R & S) cHept	63	0.37 (K)		*391 (100%)
LXXVI	74	(R & S) cHept	68	0.18 (K) 0.50 (L)	123 (Foam)	#363 (100%), 362 (90%)
LXXVII	75	(R & S) cHept	100	0.16 (K)	108	*376 (100%)

[0209]

			Yield		Melting	MS (*) (rela-
Example No.	D	R1	(% of theory)	Rf (solvent)	point (° C.)	tive intensity)
LXXVIII	76	(R & S) cPent	100	0.07 (K)	>220	419 (60%), 417 (100%)
LXXIX	77	(R & S) cPent	80	0.32 (K)	234	317 (100%)
LXXX	78	(R & S) cPent	92	0.44 (K)
LXXXI	79	(R & S) cPent	88	0.27 (K)	126 (Foam)	391 (100%)
LXXXII	80	(R & S) cPent	99	0.44 (L)	92 (Foam)	417 (100%)

[0210]

			Yield		Melting	MS (*) (rela-
Example No.	D	R1	(% of theory)	Rf (solvent)	point (° C.)	tive intensity)
LXXXVIII	81	(R & S) cPent	96	0.24 (K)	>230	378 (100%), 154 (60%)
LXXXIX	82	(R & S) cHex	71	0.27 (K)	120 (Foam)	392 (100%)
XC	83	(R & S) cPent	87	0.16 (K)	205	#454 (100%)
XCI	84	(R & S) cPent	37	0.37 (K)	160	440 (100%)

[0211] The examples listed in Table VI are prepared analogously to the instructions of Examples XXIII and LIX.

TABLE VI
85
				Melting	Yield	Rf	MS (relative
Example No.	D	R1	R15	point (° C.)	(% of theory)	(solvent)	intensity)
XCII	86	(R & S) cPent	tBu	—	30	0.37 (E)	541 (100%)  57 (60%)
XCIII	87	(R & S) cPent	H	>220	84	0.09 (K)	485 (100%)

PREPARATION EXAMPLES

Example 1

[0212] 2-(R)- and 2-(S)-2-[4-(2-phenyl-1H-benzimidazol-1-ylmethyl)-phenyl]-2-cyclopentylacetic acid (2-(R)-2-phenyl-glycinol)-amide 88

[0213] 500 mg (1.22 mmol) of the compound from Example LXXXIII and 0.68 ml of triethylamine are reacted with 0.10 ml of mesyl chloride in 15 ml of anhydrous N,N-dimethylformamide at −30° C. for 30 minutes. Thereafter, a solution of 201 mg (1.46 mmol) of 2-(R)-2-phenyl-glycinol and 149 mg (1.22 mmol) of 4-(N,N-dimethylamino)-pyridine in 15 ml of anhydrous N,N-dimethylformamide is added at −30° C. and the mixture is subsequently stirred at the stated temperature for one hour. After a total stirring time of 20 hours, with gradual warming to about 20° C., the mixture is poured onto ethyl acetate and aqueous sodium bicarbonate solution, the phases are separated and the aqueous solution is rinsed twice with ethyl acetate. The combined organic phases are then extracted in succession with water (2×), 1 M sodium hydroxide solution (3×), water (1×) and buffer of pH=2 (2×), dried with anhydrous sodium sulphate and evaporated - finally under a high vacuum.

[0214] Yield: 640 mg (1.2 mmol/99%); Rf=0.46 (methylene chloride:methanol=10:1).

Example 2

[0215] 2-Cycloheptyl-N-(2-hydroxy-1-(R)-phenylethyl)-2-[4-(2,3-dimethylindol-1-yl-methyl)phenyl]acetamide 89

[0216] 538 mg (1.38 mmol) of the compound from Example LIX are dissolved in 10 ml of CH2Cl2. 265 mg (1.38 mmol) of R-phenylglycinol are added. The solution is cooled to 0° C. and 204 mg (1.51 mmol) of 1-hydroxy-1-benzotriazole and 304 mg (1.58 mmol) of N′-(3-dimethylaminopropyl)-N-ethylcarbodiimide×HCl are subsequently added. 279 mg (2.76 mmol) of triethylamine are added dropwise. The mixture is subsequently stirred overnight at room temperature. Water and CH2Cl2 are added and the mixture is subjected to extraction. The organic phase is dried over sodium sulphate and concentrated on a rotary evaporator. The residue is purified by column chromatography (silica gel Merck 60 (0.004 - 0.063)).

[0217] Yield: 600 mg (85.4%); Melting point: 163° C.; Rf=0.77 (CH2Cl2.:methanol=10:1); Mass calculated for C34N40N2O2=508.71; mass spectrum (DCI (NH3), relative intensity) 509 (50%), 508 (100%).

[0218] The compounds listed in Table 1 are prepared analogously to the instructions of Examples 1 and 2:

TABLE 1
90
						Melt-	MS (*)
Ex-				Yield	Rf	ing	(rela-
ample				(% of	(sol-	point	ative
No.	D	R1	R15	theory)	vent)	(° C.)	intensity)
3	91	(R & S) cHept	92	75	0.69 (L)	170	481 (100%)
4	93	(R & S) cHept	94	76	0.71 (L)	163	495 (100%)
5	95	(R & S) cPent	96	57			481 (80%) 480 (100%)
6	97	(dia A) cPent	98		0.37 (J)	185

[0219]

						Melt-	MS (*)
Ex-				Yield	Rf	ing	(rela-
ample				(% of	(sol-	point	ative
No.	D	R1	R15	theory)	vent)	(° C.)	intensity)
7	99	(dia B) cPent	100		0.23 (J)	182
8	101	(R & S) cHex	102	72	0.58 (J)	180-82	494 (100%)
9	103	(dia A) cHex	104		0.54 (K)	198
10	105	(dia B) cHex	106		0.39 (K)	204
11	107	(R & S) cHex	108	46	0.37 (J)	98 (Foam)	510 (100%)
12	109	(dia A) cHex	110			195-96
13	111	(dia B) cHex	112			195-96

[0220]

						Melt-	MS (*)
Ex-				Yield	Rf	ing	(rela-
ample				(% of	(sol-	point	ative
No.	D	R1	R15	theory)	vent)	(° C.)	intensity)
14	113	(dia A) cPent	114	11	0.60 (K)	183-85	529 (100%), 528 (80%)
15	115	(dia B) cPent	116	5	0.45 (K)		529 (100%), 528 (80%)
16	117	(R & S) cHept	118	70	0.54 (K)	182-86	557 (100%)
17	119	(R & S) cPent	120	82		118	525 (100%), 171 (100%)
18	121	(dia A) cPent	122		0.54 (K)
19	123	(dia B) cPent	124		0.44 (K)
20	125	(R & S) cHept	126	81	0.52 (K)	178-81	553 (100%), 199 (100%)

[0221]

						Melt-	MS (*)
Ex-				Yield	Rf	ing	(rela-
ample				(% of	(sol-	point	ative
No.	D	R1	R15	theory)	vent)	(° C.)	intensity)
21	127	(dia A) cHept	128			204
22	129	(dia B) cHept	130			203
23	131	(R & S) cPent	132	61		116	555 (100%), 554 (90%)
24	133	(dia A) cPent	134		0.47 (K)
25	135	(dia B) cPent	136		0.38 (K)
26	137	(R & S) cPent	138	83		158	559 (60%), 171 (100%)
27	139	(dia A) cPent	140		0.48 (K)	162-65

[0222]

						Melt-	MS (*)
Ex-				Yield	Rf	ing	(rela-
ample				(% of	(sol-	point	ative
No.	D	R1	R15	theory)	vent)	(° C.)	intensity)
28	141	(dia B) cPent	142		0.38 (K)	191-93
29	143	(R & S) cHept	144	85	0.73 (L)	171	#494 (100%)
30	145	(R & S) cPent	146	50	0.53 (K)	155-61	467 (100%), 466 (80%)
31	147	(R & S) cPent	148	78	0.46 (K)	186-90	467 (100%), 466 (80%)
32	149	(R & S) cHept	150	66	0.47 (K)	153-55	515 (100%), 164 (80%)
33	151	(R & S) cPent	152	38	0.59 (K)	170-75	487 (100%)

[0223]

						Melt-	MS (*)
Ex-				Yield	Rf	ing	(rela-
ample				(% of	(sol-	point	ative
No.	D	R1	R15	theory)	vent)	(° C.)	intensity)
34	153	(dia A) cPent	154			160-62
35	155	(dia B) cPent	156			204-05
36	157	(R & S) cPent	158	71	0.52 (K)	173-76	482 (100%)
37	159	(dia A) cPent	160			183-85
38	161	(dia B) cPent	162			200-03

[0224]

				Yield		Melting	MS(*)
Example No.	D	R1	R15	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
39	163	(R&S) cHept	164	78	0.55 (K)	163-68	511 (80%), 510 (100%)
40	165	(R&S) cHept	166	86	0.25 (K)	108 (Foam)	#482 (100%)
41	167	(R&S) cHept	168	69	0.03 (K)	110 (Foam)	#496 (100%)
42	169	(R&S) cHept	170	54	0.28 (K)	230-33	538 (70%), 536 (100%)
43	171	(dia A) cPent	172
44	173	(dia B) cPent	174			>220

[0225]

				Yield		Melting	MS(*)
Example No.	D	R1	R15	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
45	175	R&S) cPent	176	83	0.32 (K)	93	496 (100%)
46	177	(R&S) cPent	178	88	0.52 (L)
47	179	(dia A) cPent	180		0.52 (L)
48	181	(dia B) cPent	182		0.52 (L)
49	183	(R&S) cPent	184	76	0.37 (K)	104 (Foam)	510 (100%), 154 (60%)
50	185	(R&S) cPent	186	90	0.52 (K)	100 (Foam)	536 (100%), 105 (55%)
51	187	(R&S) cPent	188	54	0.47 (L)		510 (100%)

[0226]

				Yield		Melting	MS(*)
Example No.	D	R1	R15	(% of theory)	Rf (solvent)	point (° C.)	(relative intensity)
52	189	(dia A) cPent	190		0.47 (L)
53	191	(dia B) cPent	192		0.43 (L)
54	193	(R&S) cPent	194	99	0.54 (L)		530 (100%)
55	195	(dia A) cPent	196		0.54 (L)
56	197	(dia B) cPent	198		0.50 (L)
57	199	(R&S) cHept	200	41	0.15 (U)	amorphous	558 (100%)
58	201	(dia A) cHept	202			191-2

[0227]

				Yield		Melting	MS(*) (re-
Example No.	D	R1	R15	(% of theory)	Rf (solvent)	point (° C.)	lative intensity)
59	203	(dia A) cHept	204			211-2
60	205	(R&S) cHept	206	56	0.31 (U)	amorphous	426 (100%)
61	207	(R&S) cPent	208	65	0.26 (K)		495 (100%)
62	209	(dia A) cPent	210			172
63	211	(dia B) cPent	212			213
64	213	(R&S) cPent	214	86	0.23 (K)		531 (100%)
65	215	(R&S) cPent	216	75			497 (100%)

[0228]

Exam-				Yield		Melting	MS(*) (rela-
ple No.	D	R1	R15	(% of theory)	Rf (solvent)	point (° C.)	tive intensity)
66	217	(dia A) cPent	218		0.20 (K)	106-108 (Foam)
67	219	(dia B) cPent	220		0.16 (K)	234
68	221	(R&S) cHex	222				511 (100%)
69	223	(R&S) cPent	224	47	0.47 (K)	186	523 (60%), 55 (100%)
70	225	(R&S) cPent	226	76	0.60 (K)	100 (Foam)	#573 (100%)
71	227	(dia A) cPent	228		0.41 (K)	100
72	229	(dia B) cPent	230		0.35 (K)	150

[0229]

Exam-				Yield		Melting	MS(*) (rela-
ple No.	D	R1	R15	(% of theory)	Rf (solvent)	point (° C.)	tive intensity)
73	231	(R&S) cPent	232	72	0.44 (K)	210	559 (100%)
74	233	(dia A) cPent	234
75	235	(dia B) cPent	236
76	237	(R&S) cPent	238	82	210-12	604 (60%), 105 (100%)
77	239	(dia A) cPent	240		229
78	241	(dia B) cPent	242		212

Claims

1. Indolyl-substituted phenylacetic acid derivatives of the general formula (I)

2. Indolyl-substituted phenylacetic acid derivatives of the formula according to claim 1, in which D represents a radical of the formula 245 wherein T denotes a nitrogen atom or the —CH group, R6, R7, R10 and R11 are identical or different and denote hydrogen, trifluoromethyl, fluorine, chlorine, bromine or straight-chain or branched alkyl or alkoxy having in each case up to 5 carbon atoms, R5, R8 and R9 are identical or different and denote hydrogen, cyclopropyl, cyclopentyl, cyclohexyl, phenyl, straight-chain or branched alkoxycarbonyl having up to 5 carbon atoms or straight-chain or branched alkyl having up to 5 carbon atoms, which is optionally substituted by fluorine, chlorine or bromine, or, in the case where T represents a nitrogen atom, R5 can also denote benzyl, E and L are identical or different and represent hydrogen, fluorine, chlorine, bromine, trifluoromethyl, hydroxyl or straight-chain or branched alkyl or alkoxy having in each case up to 4 carbon atoms, R1 represents cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl, or represents straight-chain or branched alkyl having up to 7 carbon atoms, or represents phenyl, which is optionally substituted by fluorine, chlorine, bromine, cyano, hydroxyl or straight-chain or branched alkyl or alkoxy having in each case up to 3 carbon atoms, R2 denotes hydrogen or methyl, R3 represents hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms, cyclopropyl, cyclopentyl or cyclohexyl, or represents phenyl, pyridyl, thienyl or furyl, each of which is optionally substituted up to twice in an identical or different manner by fluorine, chlorine, bromine, phenyl, nitro, hydroxyl or by straight-chain or branched alkyl or alkoxy having up to 4 carbon atoms, R4 represents hydrogen, or represents a group of the formula —CH2—OH or —CH2O—CO—R12, wherein R12 denotes hydrogen, straight-chain or branched alkyl having up to 6 carbon atoms or phenyl, which is optionally substituted up to twice in an identical or different manner by fluorine, chlorine, bromine, cyano, hydroxyl or straight-chain or branched alkyl or alkoxy having in each case up to 3 carbon atoms, and salts thereof

3. Indolyl-substituted phenylacetic acid derivatives of the formula according to claim 1, in which D represents a radical of the formula 246wherein T denotes a nitrogen atom or the —CH group, R6, R7, R10 and R11 are identical or different and denote hydrogen, trifluoromethyl, chlorine or straight-chain or branched alkyl or alkoxy having in each case up to 4 carbon atoms, R5, R8 and R9 are identical or different and denote hydrogen, cyclopropyl, cyclopentyl, cyclohexyl phenyl, straight-chain or branched alkoxycarbonyl having up to 4 carbon atoms or straight-chain or branched alkyl having up to 4 carbon atoms, which is optionally substituted by chlorine, or, in the case where T represents a nitrogen atom, R5 can also denote benzyl, E and L are identical or different and represent hydrogen, fluorine, chlorine, bromine or trifluoromethyl, R1 represents cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl, or represents straight-chain or branched alkyl having up to 6 carbon atoms, or represents phenyl, which is optionally substituted by fluorine, chlorine, cyano, hydroxyl, methyl or methoxy, R2 represents hydrogen or methyl, R3 represents hydrogen or straight-chain or branched alkyl having up to 3 carbon atoms, cyclopropyl, cyclopentyl or cyclohexyl, or represents pyridyl, thienyl or phenyl, each of which is optionally substituted up to twice in an identical or different manner by fluorine, chlorine, bromine, nitro, phenyl, hydroxyl or by straight-chain or branched alkyl or alkoxy having up to 3 carbon atoms, R4 represents hydrogen, or represents a group of the formula —CH2—OH or —CH2O—CO—R12, wherein R12 denotes hydrogen, straight-chain or branched alkyl having up to 6 carbon atoms or phenyl, which is optionally substituted up to twice in an identical or different manner by fluorine, chlorine, bromine, cyano, hydroxyl, methyl or methoxy, and salts thereof.

4. Indolyl-substituted phenylacetic acid derivatives according to claim 1 wherein such compound is 2-(R)- and 2-(S)-2-[4-(2-phenyl-1H-benzimidazol-1-ylmethyl)-phenyl]-2-cyclopentyl-aceticacid (2-(R)-2-phenyl-glycinol)-amide of the formula

5. Indolyl-substituted phenylacetic acid derivatives according to claim 1 wherein such compound is 2-Cycloheptyl-N-(2-hydroxy-1-(R)-phenylethyl)-2-[4-(2,3-dimethylindol-1-yl-methyl)phenyl]acetamide of the fromula

6. Indolyl-substituted phenylacetic acid derivatives according to claim 1 wherein such compound is 2-(R) and 2(S)-cycloheptyl-N-(2-hydroxy-1-(R)-phenylethyl)-2-[4-(2-carboethoxyindol-1-ylmethyl)-phenyl]acetamide of the formula

7. Indolyl-substituted phenylacetic acid derivatives according to claim 1 wherein such compound is 2-(R) and 2(S)-cycloheptyl-N-(2-hydroxy-1-(R)-phenylethyl)-2-[4-(2-phenylindol-1-ylmethyl)-phenyl]acetamide of the formula

8. Indolyl-substituted phenylacetic acid derivatives according to claim 1 wherein such compound is 2-(R) and 2(S)-cyclopentyl-N-(2-hydroxy-1-(R)-phenylethyl)-2-[4-(2,5,7-trimethyl-imidazo[4,5-b]pyridin-3-ylmethyl)-phenyl]of the formula

9. A composition for the treatment of arteriosclerosis comprising an amount effective therefore of a compound or salt thereof according to claim 1 and a pharmacologically acceptable diluent.

10. The method for treating arteriosclerosis in a patient in need thereof which comprises administering to such patient an amount effective therefor or a salt thereof according to claim 1.